O. O. Lun’ko, D. S. Isaiev, O. P. Maxymiuk, O. O. Kryshtal', O. V. Isaieva
We investigated the effect of proteases, widely used for neuron isolation in electrophysiological studies, on the amplitude and kinetic characteristics of persistent sodium current (I(NaP)) in hippocampal CA1 pyramidal neurons. Properties of I(NaP) were studied on neurons isolated by mechanical treatment (control group) and by mechanical and enzymatic treatment using pronase E (from Streptomyces griseus) or protease type XXIII (from Aspergillus oryzae). We show that in neurons isolated with pronase E kinetic of activation and density of I(NaP) was unaltered. Enzymatic treatment with protease type XXIII did not alter I(NaP) activation but result in significant decrease in I(NaP) density. Our data indicates that enzymatic treatment using pronase E for neuron isolation is preferable for investigation of I(NaP).
{"title":"[The effect of enzymatic treatment using proteases on properties of persistent sodium current in CA1 pyramidal neurons of rat hippocampus].","authors":"O. O. Lun’ko, D. S. Isaiev, O. P. Maxymiuk, O. O. Kryshtal', O. V. Isaieva","doi":"10.15407/fz60.03.075","DOIUrl":"https://doi.org/10.15407/fz60.03.075","url":null,"abstract":"We investigated the effect of proteases, widely used for neuron isolation in electrophysiological studies, on the amplitude and kinetic characteristics of persistent sodium current (I(NaP)) in hippocampal CA1 pyramidal neurons. Properties of I(NaP) were studied on neurons isolated by mechanical treatment (control group) and by mechanical and enzymatic treatment using pronase E (from Streptomyces griseus) or protease type XXIII (from Aspergillus oryzae). We show that in neurons isolated with pronase E kinetic of activation and density of I(NaP) was unaltered. Enzymatic treatment with protease type XXIII did not alter I(NaP) activation but result in significant decrease in I(NaP) density. Our data indicates that enzymatic treatment using pronase E for neuron isolation is preferable for investigation of I(NaP).","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"8 1","pages":"75-9"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85145963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-01-01DOI: 10.1615/INTJPHYSPATHOPHYS.V6.I3.40
K. Drachuk, A. Kotsiuruba, O. V. Baziliuk, L. H. Stepanenko, V. F. Sahach
In the study we investigated the effect of blockade cystathionine-gamma -lyase (CSE), an enzyme of hydrogen sulfide (H2S) (de novo) synthesis on the endothelium-dependent relaxation of aortic smooth muscle (SM) in old rats. It has been shown that an inhibition of CSE by propargylglycine (PAG) results in restoration of a decreased ACh-induced relaxation of aorta in old rats. This effect of PAG was removed by blocking nitric oxide (NO) synthesis in the endothelial cells. Age-related changes in the levels of H2S, NO2- and enzyme activity of the constitutive synthesis of NO (cNOS) in the heart, were determined. It has been shown that PAG introduction elevates a decreased levels of HzS, NO2- and stimulates the suppressed activity of cNOS in old rats. These results suggest that PAG activates alternative ways of H2S synthesis and stimulates the constitutive synthesis of NO. These actions of PAG restore endothelial function in old rats.
{"title":"[Propargylglycine restores endothelium-dependent relaxation of aortic smooth muscles in old rats].","authors":"K. Drachuk, A. Kotsiuruba, O. V. Baziliuk, L. H. Stepanenko, V. F. Sahach","doi":"10.1615/INTJPHYSPATHOPHYS.V6.I3.40","DOIUrl":"https://doi.org/10.1615/INTJPHYSPATHOPHYS.V6.I3.40","url":null,"abstract":"In the study we investigated the effect of blockade cystathionine-gamma -lyase (CSE), an enzyme of hydrogen sulfide (H2S) (de novo) synthesis on the endothelium-dependent relaxation of aortic smooth muscle (SM) in old rats. It has been shown that an inhibition of CSE by propargylglycine (PAG) results in restoration of a decreased ACh-induced relaxation of aorta in old rats. This effect of PAG was removed by blocking nitric oxide (NO) synthesis in the endothelial cells. Age-related changes in the levels of H2S, NO2- and enzyme activity of the constitutive synthesis of NO (cNOS) in the heart, were determined. It has been shown that PAG introduction elevates a decreased levels of HzS, NO2- and stimulates the suppressed activity of cNOS in old rats. These results suggest that PAG activates alternative ways of H2S synthesis and stimulates the constitutive synthesis of NO. These actions of PAG restore endothelial function in old rats.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"23 1","pages":"3-10"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85246465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. P. Vasheka, S. P. Vesel'skyĭ, Z. A. Horenko, O. A. Hrinchenko, L. S. Karbovs'ka, M. Makarchuk
In acute experiments on the rats with cannulated common biliary duct, the influence ofamylin on the level of bile secretion and bile acids spectrum was investigated. It was shown that subcutaneous administration of amylin at the dose 1 mg/kg body weight doesn't affect the volume of secreted bile. Under these conditions, the concentration of taurocholic acid was increased and the concentration of tauroconjugates of chenodeoxycholic and deoxycholic acids was decreased in the bile. At the same time, the concentration of glycocholates remained constant and of unconjugated bile acids was decreased. This redistribution of bile acids spectrum leads to an increase in the coefficient of conjugation. Amylin changes the ratio of trygydroxy- and dygydroxycholates in secreted bile leading to an increase in the coefficient of hydroxylation. These results suggest that amylin enhances the processes of conjugation and hydroxylation of bile acids in hepatocytes that results in improvement of detergent properties of the bile, particularly, the ability of the bile to maintain the cholesterol in dissolved state. At the lowest effective dose, amylin does not alter the concentration of glucose in the blood.
{"title":"[The influence of amylin on the bile acid spectrum in rats].","authors":"I. P. Vasheka, S. P. Vesel'skyĭ, Z. A. Horenko, O. A. Hrinchenko, L. S. Karbovs'ka, M. Makarchuk","doi":"10.15407/FZ60.03.046","DOIUrl":"https://doi.org/10.15407/FZ60.03.046","url":null,"abstract":"In acute experiments on the rats with cannulated common biliary duct, the influence ofamylin on the level of bile secretion and bile acids spectrum was investigated. It was shown that subcutaneous administration of amylin at the dose 1 mg/kg body weight doesn't affect the volume of secreted bile. Under these conditions, the concentration of taurocholic acid was increased and the concentration of tauroconjugates of chenodeoxycholic and deoxycholic acids was decreased in the bile. At the same time, the concentration of glycocholates remained constant and of unconjugated bile acids was decreased. This redistribution of bile acids spectrum leads to an increase in the coefficient of conjugation. Amylin changes the ratio of trygydroxy- and dygydroxycholates in secreted bile leading to an increase in the coefficient of hydroxylation. These results suggest that amylin enhances the processes of conjugation and hydroxylation of bile acids in hepatocytes that results in improvement of detergent properties of the bile, particularly, the ability of the bile to maintain the cholesterol in dissolved state. At the lowest effective dose, amylin does not alter the concentration of glucose in the blood.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"195 1","pages":"46-53"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76950650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-01-01DOI: 10.1615/INTJPHYSPATHOPHYS.V6.I2.90
I. Litovka, Y. O. Mazepa-Kryzhanivs'ka, V. Berezovskyi
In a review of the current literature for the 2000-2013 period, the data of the effect of melatonin on organic and inorganic matrix of bone tissue have been presented. These results suggest that melatonin is an important mediator in the formation of bone tissue. It can prevent the premature destruction of bone and promote its recovery through mechanisms of melatonin-related receptors and receptors of independent action.
{"title":"[The effect of melatonin on bone tissue metabolism].","authors":"I. Litovka, Y. O. Mazepa-Kryzhanivs'ka, V. Berezovskyi","doi":"10.1615/INTJPHYSPATHOPHYS.V6.I2.90","DOIUrl":"https://doi.org/10.1615/INTJPHYSPATHOPHYS.V6.I2.90","url":null,"abstract":"In a review of the current literature for the 2000-2013 period, the data of the effect of melatonin on organic and inorganic matrix of bone tissue have been presented. These results suggest that melatonin is an important mediator in the formation of bone tissue. It can prevent the premature destruction of bone and promote its recovery through mechanisms of melatonin-related receptors and receptors of independent action.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"33 1","pages":"102-9"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84288279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Maslak, O. Kostiuk, D. Minchenko, O. Brazaluk, A. Shevtsova, O. Minchenko
We studied the levels of lymphocyte surface-associated sialylglycans and the expression of neuraminidase NEU1 and sialyltransferase ST6GAL1 genes in lymphocytes in erythremia patients and healthy donors as well as the levels of sialic acids in plasma and sialylation of alpha-acid glycoprotein and fibronectin. Moreover, we also investigated the type of sialic acids binding with its glycans using sialospecific lectins MAA and SNA. fibronectin protein in lymphocytes and its cell surface in erythremia disease as compared to healthy donors. It was shown that the levels of free sialic acids and neuraminidase activity in plasma are increased in erythremia disease as compared to healthy donors; however, MAA-II-binding activity of tested glycoproteins is decreased, fibronectin-1 mRNA expression in lymphocytes is increased in patients with erythremia. The decreasing of plasma fibronectin concentration and its heparin-binding activity as well as increasing of lymphocyte content with surface-associated and intracellular fibronectin were revealed in erythremia disease in comparison with healthy donors. Positive correlation between plasma fibronectin level and its heparin-binding activity and negative correlation between plasma fibronectin level and quantity of lymphocytes which express fibronectin inside the cell and on cell surface was detected. Enhanced levels of α2,3- and α2,6-linked residues of glycocojugates were detected on lymphocyte cell surface in erythremia disease using sialospecific lectins and flow cytometer as well as fluorescent confocal microscope. The level of NEU1 and ST6GAL1 mRNA expressions is significantly increased in lymphocytes in erythremia disease. Results of this study are clarified the mechanisms of disturbed in erythremia disease glycobiological processes and may therefore present new approaches for therapeutic opportunities.
{"title":"[Glycoprotein sialylation and NEU1 and ST6GAL1 expressions in erythremia].","authors":"H. Maslak, O. Kostiuk, D. Minchenko, O. Brazaluk, A. Shevtsova, O. Minchenko","doi":"10.15407/FZ60.05.014","DOIUrl":"https://doi.org/10.15407/FZ60.05.014","url":null,"abstract":"We studied the levels of lymphocyte surface-associated sialylglycans and the expression of neuraminidase NEU1 and sialyltransferase ST6GAL1 genes in lymphocytes in erythremia patients and healthy donors as well as the levels of sialic acids in plasma and sialylation of alpha-acid glycoprotein and fibronectin. Moreover, we also investigated the type of sialic acids binding with its glycans using sialospecific lectins MAA and SNA. fibronectin protein in lymphocytes and its cell surface in erythremia disease as compared to healthy donors. It was shown that the levels of free sialic acids and neuraminidase activity in plasma are increased in erythremia disease as compared to healthy donors; however, MAA-II-binding activity of tested glycoproteins is decreased, fibronectin-1 mRNA expression in lymphocytes is increased in patients with erythremia. The decreasing of plasma fibronectin concentration and its heparin-binding activity as well as increasing of lymphocyte content with surface-associated and intracellular fibronectin were revealed in erythremia disease in comparison with healthy donors. Positive correlation between plasma fibronectin level and its heparin-binding activity and negative correlation between plasma fibronectin level and quantity of lymphocytes which express fibronectin inside the cell and on cell surface was detected. Enhanced levels of α2,3- and α2,6-linked residues of glycocojugates were detected on lymphocyte cell surface in erythremia disease using sialospecific lectins and flow cytometer as well as fluorescent confocal microscope. The level of NEU1 and ST6GAL1 mRNA expressions is significantly increased in lymphocytes in erythremia disease. Results of this study are clarified the mechanisms of disturbed in erythremia disease glycobiological processes and may therefore present new approaches for therapeutic opportunities.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"42 1","pages":"14-22"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84583933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Fomenko, T. Bondarchuk, L. P. Bilets'ka, N. B. Panasiuk, O. Skliarov
In experiments on rats with modeled water-restrained stress, the influence of nonsteroidal anti-inflammatory drugs of different genesis on morphological status of gastric mucosa and changes of NO-synthase system parameters have been studied Administration of nonselective cyclooxygenese inhibitor naproxen in the water-restrained stress model in rats potentiated the increase of severity of damage of gastric mucosa. At the same time, the activity of both inducible and constitutive isoforms ofNO-sythase decreased. The parameters of lipoperoxidation remained at the level observed during water-restrained stress. It was shown the advantages of the use of H2S-releasinfg nonsteroidal anti-inflammatory drug ATB-346, which are associated with its cytoprotective effect of the drug manifested by a decreased total area of gastric damage. However, parameters of lipoperoxidation and NO-syntase system did not differ substantially from those in the group treated with napoxen, indicating the prevalence of parent molecule (naproxen) in regulation of function of NO-system Administration of dual COX/LOX inhibitor, the compound 2A5DHT, caused a decrease of gastric damage as compared to the effect ofnaproxen. The activity of iNOS remained much higher than under condition of the naproxen action.
{"title":"[Parameters of NO synthase system of gastric mucosa in rats under stress conditions and inhibition of cyclooxygenase].","authors":"I. Fomenko, T. Bondarchuk, L. P. Bilets'ka, N. B. Panasiuk, O. Skliarov","doi":"10.15407/FZ60.02.051","DOIUrl":"https://doi.org/10.15407/FZ60.02.051","url":null,"abstract":"In experiments on rats with modeled water-restrained stress, the influence of nonsteroidal anti-inflammatory drugs of different genesis on morphological status of gastric mucosa and changes of NO-synthase system parameters have been studied Administration of nonselective cyclooxygenese inhibitor naproxen in the water-restrained stress model in rats potentiated the increase of severity of damage of gastric mucosa. At the same time, the activity of both inducible and constitutive isoforms ofNO-sythase decreased. The parameters of lipoperoxidation remained at the level observed during water-restrained stress. It was shown the advantages of the use of H2S-releasinfg nonsteroidal anti-inflammatory drug ATB-346, which are associated with its cytoprotective effect of the drug manifested by a decreased total area of gastric damage. However, parameters of lipoperoxidation and NO-syntase system did not differ substantially from those in the group treated with napoxen, indicating the prevalence of parent molecule (naproxen) in regulation of function of NO-system Administration of dual COX/LOX inhibitor, the compound 2A5DHT, caused a decrease of gastric damage as compared to the effect ofnaproxen. The activity of iNOS remained much higher than under condition of the naproxen action.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"11 1","pages":"51-6"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89586881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-01-01DOI: 10.1615/INTJPHYSPATHOPHYS.V6.I4.30
O. Kolomiiets, Iu V Danylovych, H. V. Danylovych
The properties of ΔpH-induced Ca2+-transport from isolated rat myometrium mitochondria was investigated. Ca2+-accu- mulation was carried out in the presence of Mg-ATP2- and succinate. Transport of Ca2+ recorded using Ca2+-sensitive fluorescent probe Fluo-4 AM. It is shown that acidification of extramitochondrial medium is accompanied by stimulation of Ca2+ release from mitochondria. This process is insensitive to the tetraphenylphosphonium which is relatively specific Na+-Ca2+-exchanger inhibitor of mitochondrial inner membrane, but inhibited in the presence of monoclonal antibodies directed against the protein LETM1 (Anti-LETM1). LETM1 protein in some tissues is the molecular basis of the H+-Ca2+-exchanger functioning on mitochondria. It was found that the H+-Ca2+-exchanger is stimulated by 100 μM amiloride (diuretic) and inhibited by Mg ions in milimolar concentrations. The transport system was completely resistant to the action of nitric oxide (sodium nitroprusside and sodium nitrite), but was stimulated by macrocyclic compounds of Calixarenes (C-97 and C-99) in submicromolar concentrations. Thus, the mitochondria of rat myometrium probably not have a system of Na+-Ca2+-exchanger, and provide the maintenance of the matrix Ca2+-homeostasis with H+-Ca2+-exchanger. Since the transport system high affinity activated by Calixarenes, further investigation of the influence of these compounds on the transport process makes promising.
{"title":"[H+-Ca2+-exchanger in the myometrium mitochondria: modulation of exogenous and endogenous compounds].","authors":"O. Kolomiiets, Iu V Danylovych, H. V. Danylovych","doi":"10.1615/INTJPHYSPATHOPHYS.V6.I4.30","DOIUrl":"https://doi.org/10.1615/INTJPHYSPATHOPHYS.V6.I4.30","url":null,"abstract":"The properties of ΔpH-induced Ca2+-transport from isolated rat myometrium mitochondria was investigated. Ca2+-accu- mulation was carried out in the presence of Mg-ATP2- and succinate. Transport of Ca2+ recorded using Ca2+-sensitive fluorescent probe Fluo-4 AM. It is shown that acidification of extramitochondrial medium is accompanied by stimulation of Ca2+ release from mitochondria. This process is insensitive to the tetraphenylphosphonium which is relatively specific Na+-Ca2+-exchanger inhibitor of mitochondrial inner membrane, but inhibited in the presence of monoclonal antibodies directed against the protein LETM1 (Anti-LETM1). LETM1 protein in some tissues is the molecular basis of the H+-Ca2+-exchanger functioning on mitochondria. It was found that the H+-Ca2+-exchanger is stimulated by 100 μM amiloride (diuretic) and inhibited by Mg ions in milimolar concentrations. The transport system was completely resistant to the action of nitric oxide (sodium nitroprusside and sodium nitrite), but was stimulated by macrocyclic compounds of Calixarenes (C-97 and C-99) in submicromolar concentrations. Thus, the mitochondria of rat myometrium probably not have a system of Na+-Ca2+-exchanger, and provide the maintenance of the matrix Ca2+-homeostasis with H+-Ca2+-exchanger. Since the transport system high affinity activated by Calixarenes, further investigation of the influence of these compounds on the transport process makes promising.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"85 1","pages":"33-42"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84087137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-01-01DOI: 10.1615/INTJPHYSPATHOPHYS.V6.I3.10
O. Shandra
The aim of the work was investigation of both humoral and cell-mediated immunity together with leukocytes functional stability indexes in rats with the experimental contact dermatitis (ECD) in conditions of complex pharmacological correction using deltaran and melatonin. Experimental trials were performed under conditions of chronic experiment on model chrome-induced ECD. Both deltaran and melatonin either alone or in combination were used for complex pharmacological correction of humoral and cell-mediated immunity and also for stability of leukocytes. The data obtained showed the expressed disturbances of humoral and cell-mediated immunity and neutrophils' functional stability damage under conditions of chrome-induced ECD in rats. The revealed alterations in functional activity of the immune system were successfully corrected using the combined administration of deltaran and melatonin. The activity of medical complex had exponential character.
{"title":"[Effect of deltaran and melatonin on immune system in rats with experimental contact dermatitis].","authors":"O. Shandra","doi":"10.1615/INTJPHYSPATHOPHYS.V6.I3.10","DOIUrl":"https://doi.org/10.1615/INTJPHYSPATHOPHYS.V6.I3.10","url":null,"abstract":"The aim of the work was investigation of both humoral and cell-mediated immunity together with leukocytes functional stability indexes in rats with the experimental contact dermatitis (ECD) in conditions of complex pharmacological correction using deltaran and melatonin. Experimental trials were performed under conditions of chronic experiment on model chrome-induced ECD. Both deltaran and melatonin either alone or in combination were used for complex pharmacological correction of humoral and cell-mediated immunity and also for stability of leukocytes. The data obtained showed the expressed disturbances of humoral and cell-mediated immunity and neutrophils' functional stability damage under conditions of chrome-induced ECD in rats. The revealed alterations in functional activity of the immune system were successfully corrected using the combined administration of deltaran and melatonin. The activity of medical complex had exponential character.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"182 1","pages":"78-83"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80330242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Leshchenko, V. Shevchuk, O. Savcheniuk, T. Falalieieva, S. Sukhodolia, T. Berehova
The influence of neonatal administration of hyperosmolar sodium chloride and sodium glutamate on the exocrine function of the pancreas in rats has been investigated. It was observed the development of acute pancreatitis under experimental obesity. The cross-section area of acini reduced by 12%, the cross-section area of acinocytes nuclei increased by 10%, the length between the lobes of the gland has grown by 48%. The level of amylase was increased by 43%, the levels of pancreatic amylase and lipase were increased by 68% and 24%, respectively.
{"title":"[Exocrine function of the pancreas in rats with experimental obesity].","authors":"I. Leshchenko, V. Shevchuk, O. Savcheniuk, T. Falalieieva, S. Sukhodolia, T. Berehova","doi":"10.15407/FZ60.01.041","DOIUrl":"https://doi.org/10.15407/FZ60.01.041","url":null,"abstract":"The influence of neonatal administration of hyperosmolar sodium chloride and sodium glutamate on the exocrine function of the pancreas in rats has been investigated. It was observed the development of acute pancreatitis under experimental obesity. The cross-section area of acini reduced by 12%, the cross-section area of acinocytes nuclei increased by 10%, the length between the lobes of the gland has grown by 48%. The level of amylase was increased by 43%, the levels of pancreatic amylase and lipase were increased by 68% and 24%, respectively.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"35 1","pages":"41-8"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77627788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Iu V Danylovych, O. Kolomiiets, H. V. Danylovych, S. Kosterin
The influence of the donor and the precursor of NO, namely 100 mM sodium nitroprusside and sodium nitrite on the energo-dependent Ca(2+)-transport in isolated mitochondria from rat myometrium was investigated. Changes in the mitochondrial matrix Ca(2+)-concentration was evaluated by spectrofluorimetry using Ca2+ sensitive probe Fluo-4 AM. Mg(2+)-ATP-dependent Ca(2+)-accumulation on mitochondria in the presence of succinate significantly stimulated by nitric oxide, in particular, 100 microM sodium nitroprusside amplified the transport by 1.6 times relative to its control values. NO effect becomes significant only when the incubation of mitochondria with the compounds was performed. Ca(2+)-accumulation in the presence of sodium nitroprusside effectively suppressed by protonophore (CCCP) and ruthenium red (10 microM). It was concluded that inner mitochondrial membrane Ca(2+)-uniporter stimulated by nitrogen oxide. Ca(2+)-accumulation in mitochondria in the presence of sodium nitroprusside was not sensitive to the action of a specific permeability transition pore inhibitor cyclosporine (5 microM). This data indicates that the role of permeability transition pore is less significant than Ca(2+)-uniporter in the processes of Ca(2+)-transport in mitochondria under the nitric oxide action. Thus, nitric oxide stimulates the energo-dependent Ca(2+)-accumulation by myometrium mitochondria mediated their inner membrane Ca(2+)-uniporter functioning.
{"title":"[Nitric oxide as a possible regulator of energy-dependent Ca2+ transport in mitochondria of uterine smooth muscle].","authors":"Iu V Danylovych, O. Kolomiiets, H. V. Danylovych, S. Kosterin","doi":"10.15407/FZ60.02.012","DOIUrl":"https://doi.org/10.15407/FZ60.02.012","url":null,"abstract":"The influence of the donor and the precursor of NO, namely 100 mM sodium nitroprusside and sodium nitrite on the energo-dependent Ca(2+)-transport in isolated mitochondria from rat myometrium was investigated. Changes in the mitochondrial matrix Ca(2+)-concentration was evaluated by spectrofluorimetry using Ca2+ sensitive probe Fluo-4 AM. Mg(2+)-ATP-dependent Ca(2+)-accumulation on mitochondria in the presence of succinate significantly stimulated by nitric oxide, in particular, 100 microM sodium nitroprusside amplified the transport by 1.6 times relative to its control values. NO effect becomes significant only when the incubation of mitochondria with the compounds was performed. Ca(2+)-accumulation in the presence of sodium nitroprusside effectively suppressed by protonophore (CCCP) and ruthenium red (10 microM). It was concluded that inner mitochondrial membrane Ca(2+)-uniporter stimulated by nitrogen oxide. Ca(2+)-accumulation in mitochondria in the presence of sodium nitroprusside was not sensitive to the action of a specific permeability transition pore inhibitor cyclosporine (5 microM). This data indicates that the role of permeability transition pore is less significant than Ca(2+)-uniporter in the processes of Ca(2+)-transport in mitochondria under the nitric oxide action. Thus, nitric oxide stimulates the energo-dependent Ca(2+)-accumulation by myometrium mitochondria mediated their inner membrane Ca(2+)-uniporter functioning.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"19 1","pages":"12-7"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87197121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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