首页 > 最新文献

Folia microbiologica最新文献

英文 中文
Development of point-of-need colourimetric, isothermal diagnostic assays for specific detection of Bacillus subtilis using shikimate dehydrogenase gene. 利用莽草酸脱氢酶基因开发特异性检测枯草芽孢杆菌的需求点比色等温诊断测定法。
IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-09-27 DOI: 10.1007/s12223-024-01201-z
Nanditha S, Manjunatha C, Shivakumara K T, Ramya R S, Kandan A, Prasannakumar M K, Pramesh D, Sushil S N

The largest obstacle in the promotion of biopesticides is the existence of counterfeit products available in the market. Identification and quantification of antagonistic organisms in biopesticide products are the key to the reduction of spurious microbial pesticides. In this study, we have developed a simple, sensitive, isothermal-based colourimetric assay for specific detection of Bacillus subtilis from the biopesticide formulations and soil samples. A region specific to B. subtilis which codes for shikimate dehydrogenase was identified through in silico analysis. We employed conventional PCR, loop-mediated isothermal amplification (LAMP), recombinase polymerase amplification (RPA), and qPCR for specific detection of B. subtilis in soil samples and biopesticide formulations. Specificity tests showed that the PCR primers amplified an amplicon of 521 bp in four strains of B. subtilis only, and no amplification was found in negative control samples. Similarly, the LAMP assay showed sky blue colour in all four strains of B. subtilis and violet colour in negative control samples. Whereas in the RPA assay, upon the addition of SYBR Green dye, a bright green colour was seen in B. subtilis strains, while a brick-red colour was observed in negative control samples by visualizing under a UV transilluminator. The qPCR assay showed specific amplifications with a Ct value of 12 for B. subtilis strains and no amplification in negative control samples. In the sensitivity test, PCR could amplify DNA of B. subtilis up to 500 pg/µL. DNA concentration as low as 10 pg/µL was enough to show the colour change in the LAMP as well as the RPA assays, whereas the qPCR assay showed sensitivity till 100 pg/µL. All four diagnostic assays developed in the study have been validated in soil samples and B. subtilis-based biopesticides. Compared to conventional PCR, the qPCR assay has the advantage of quantification and visualizing the result in real-time, whereas LAMP and RPA assays have the benefits of being colourimetric and less time-consuming. The other advantages are that the results can be visualized with the naked eye, and these assays do not require a costly thermal cycler and gel documentation system. Hence, LAMP and RPA assays are highly suitable for developing point-of-need diagnostic kits and, in turn, help regulators assess the quality of biopesticides in the market.

推广生物农药的最大障碍是市场上存在假冒产品。生物农药产品中拮抗生物的鉴定和定量是减少假冒微生物农药的关键。在这项研究中,我们开发了一种简单、灵敏、基于等温比色法的检测方法,用于特异性检测生物农药制剂和土壤样品中的枯草芽孢杆菌。我们通过硅学分析确定了枯草芽孢杆菌的一个特异性区域,该区域编码莽草酸脱氢酶。我们采用了常规 PCR、环介导等温扩增(LAMP)、重组酶聚合酶扩增(RPA)和 qPCR 等方法来特异性检测土壤样品和生物农药制剂中的枯草杆菌。特异性测试表明,PCR 引物仅在四种枯草杆菌菌株中扩增出 521 bp 的扩增片段,而在阴性对照样品中未发现扩增。同样,LAMP 检测在所有四株枯草杆菌中都显示出天蓝色,在阴性对照样品中显示出紫色。而在 RPA 检测中,加入 SYBR Green 染料后,枯草杆菌菌株呈亮绿色,而在紫外透射光下观察,阴性对照样品呈砖红色。qPCR 检测显示,枯草杆菌菌株有特异性扩增,Ct 值为 12,而阴性对照样本没有扩增。在灵敏度测试中,PCR 对枯草杆菌 DNA 的扩增可达 500 pg/µL。DNA 浓度低至 10 pg/µL 就足以在 LAMP 和 RPA 检测中显示颜色变化,而 qPCR 检测的灵敏度则高达 100 pg/µL。研究中开发的所有四种诊断测定方法都已在土壤样本和基于枯草杆菌的生物农药中得到验证。与传统的 PCR 相比,qPCR 检测法的优点是可实时定量并显示结果,而 LAMP 和 RPA 检测法的优点是可比色且耗时较少。其他优点还包括:肉眼就能看到检测结果,而且这些检测不需要昂贵的热循环仪和凝胶记录系统。因此,LAMP 和 RPA 检测方法非常适合开发需求点诊断试剂盒,进而帮助监管机构评估市场上生物农药的质量。
{"title":"Development of point-of-need colourimetric, isothermal diagnostic assays for specific detection of Bacillus subtilis using shikimate dehydrogenase gene.","authors":"Nanditha S, Manjunatha C, Shivakumara K T, Ramya R S, Kandan A, Prasannakumar M K, Pramesh D, Sushil S N","doi":"10.1007/s12223-024-01201-z","DOIUrl":"https://doi.org/10.1007/s12223-024-01201-z","url":null,"abstract":"<p><p>The largest obstacle in the promotion of biopesticides is the existence of counterfeit products available in the market. Identification and quantification of antagonistic organisms in biopesticide products are the key to the reduction of spurious microbial pesticides. In this study, we have developed a simple, sensitive, isothermal-based colourimetric assay for specific detection of Bacillus subtilis from the biopesticide formulations and soil samples. A region specific to B. subtilis which codes for shikimate dehydrogenase was identified through in silico analysis. We employed conventional PCR, loop-mediated isothermal amplification (LAMP), recombinase polymerase amplification (RPA), and qPCR for specific detection of B. subtilis in soil samples and biopesticide formulations. Specificity tests showed that the PCR primers amplified an amplicon of 521 bp in four strains of B. subtilis only, and no amplification was found in negative control samples. Similarly, the LAMP assay showed sky blue colour in all four strains of B. subtilis and violet colour in negative control samples. Whereas in the RPA assay, upon the addition of SYBR Green dye, a bright green colour was seen in B. subtilis strains, while a brick-red colour was observed in negative control samples by visualizing under a UV transilluminator. The qPCR assay showed specific amplifications with a Ct value of 12 for B. subtilis strains and no amplification in negative control samples. In the sensitivity test, PCR could amplify DNA of B. subtilis up to 500 pg/µL. DNA concentration as low as 10 pg/µL was enough to show the colour change in the LAMP as well as the RPA assays, whereas the qPCR assay showed sensitivity till 100 pg/µL. All four diagnostic assays developed in the study have been validated in soil samples and B. subtilis-based biopesticides. Compared to conventional PCR, the qPCR assay has the advantage of quantification and visualizing the result in real-time, whereas LAMP and RPA assays have the benefits of being colourimetric and less time-consuming. The other advantages are that the results can be visualized with the naked eye, and these assays do not require a costly thermal cycler and gel documentation system. Hence, LAMP and RPA assays are highly suitable for developing point-of-need diagnostic kits and, in turn, help regulators assess the quality of biopesticides in the market.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142344481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optimization of fermentation conditions for the production of recombinant feruloyl esterase BpFaeT132C−D143C 优化生产重组阿魏酰酯酶 BpFaeT132C-D143C 的发酵条件
IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-09-16 DOI: 10.1007/s12223-024-01197-6
Jinghao Ma, Rana Abdul Basit, Sihan Yuan, Xuan Zhao, Xiaoyan Liu, Guangsen Fan

Feruloyl esterases (FAEs) are a crucial component of the hemicellulose-degrading enzyme family that facilitates the degradation of lignocellulose while releasing hydroxycinnamic acids such as ferulic acid with high added value. Currently, the low enzyme yield of FAEs is one of the primary factors limiting its application. Therefore, in this paper, we optimized the fermentation conditions for the expression of FAE BpFaeT132C−D143C with excellent thermal stability in Escherichia coli by experimental design. Firstly, we explored the effects of 11 factors such as medium type, isopropyl-β-d-thiogalactopyranoside (IPTG) concentration, and inoculum size on BpFaeT132C−D143C activity separately by the single factor design. Then, the significance of the effects of seven factors, such as post-induction temperature, shaker rotational speed, and inoculum size on BpFaeT132C−D143C activity, was analyzed by Plackett–Burman design. We identified the main factors affecting the fermentation conditions of E. coli expressing BpFaeT132C−D143C as post-induction temperature, pre-induction period, and post-induction period. Finally, we used the steepest ascent path design and response surface method to optimize the levels of these three factors further. Under the optimal conditions, the activity of BpFaeT132C−D143C was 3.58 U/ml, which was a significant 6.6-fold increase compared to the pre-optimization (0.47 U/ml), demonstrating the effectiveness of this optimization process. Moreover, BpFaeT132C−D143C activity was 1.52 U/ml in a 3-l fermenter under the abovementioned optimal conditions. It was determined that the expression of BpFaeT132C−D143C in E. coli was predominantly intracellular in the cytoplasm. This study lays the foundation for further research on BpFaeT132C−D143C in degrading agricultural waste transformation applications.

阿魏酰酯酶(FAEs)是半纤维素降解酶家族中的重要组成部分,可促进木质纤维素的降解,同时释放出具有高附加值的羟基肉桂酸(如阿魏酸)。目前,FAEs 产酶量低是限制其应用的主要因素之一。因此,本文通过实验设计优化了在大肠杆菌中表达具有优异热稳定性的 FAE BpFaeT132C-D143C 的发酵条件。首先,通过单因素设计分别探讨了培养基类型、异丙基-β-d-硫代半乳糖苷(IPTG)浓度、接种量等 11 个因素对 BpFaeT132C-D143C 活性的影响。然后,通过普拉克特-伯曼设计分析了诱导后温度、摇床转速和接种物大小等七个因素对 BpFaeT132C-D143C 活性的显著性影响。我们确定了影响表达 BpFaeT132C-D143C 的大肠杆菌发酵条件的主要因素为诱导后温度、诱导前时间和诱导后时间。最后,我们采用最陡上升路径设计和响应面法进一步优化了这三个因素的水平。在最优条件下,BpFaeT132C-D143C 的活性为 3.58 U/ml ,与优化前(0.47 U/ml )相比,显著提高了 6.6 倍,证明了该优化过程的有效性。此外,在上述优化条件下,3 升发酵罐中的 BpFaeT132C-D143C 活性为 1.52 U/ml 。经测定,BpFaeT132C-D143C 在大肠杆菌中的表达主要在细胞质内。这项研究为进一步研究 BpFaeT132C-D143C 在降解农业废弃物转化方面的应用奠定了基础。
{"title":"Optimization of fermentation conditions for the production of recombinant feruloyl esterase BpFaeT132C−D143C","authors":"Jinghao Ma, Rana Abdul Basit, Sihan Yuan, Xuan Zhao, Xiaoyan Liu, Guangsen Fan","doi":"10.1007/s12223-024-01197-6","DOIUrl":"https://doi.org/10.1007/s12223-024-01197-6","url":null,"abstract":"<p>Feruloyl esterases (FAEs) are a crucial component of the hemicellulose-degrading enzyme family that facilitates the degradation of lignocellulose while releasing hydroxycinnamic acids such as ferulic acid with high added value. Currently, the low enzyme yield of FAEs is one of the primary factors limiting its application. Therefore, in this paper, we optimized the fermentation conditions for the expression of FAE BpFae<sup>T132C−D143C</sup> with excellent thermal stability in <i>Escherichia coli</i> by experimental design. Firstly, we explored the effects of 11 factors such as medium type, isopropyl-β-<span>d</span>-thiogalactopyranoside (IPTG) concentration, and inoculum size on BpFae<sup>T132C−D143C</sup> activity separately by the single factor design. Then, the significance of the effects of seven factors, such as post-induction temperature, shaker rotational speed, and inoculum size on BpFae<sup>T132C−D143C</sup> activity, was analyzed by Plackett–Burman design. We identified the main factors affecting the fermentation conditions of <i>E. coli</i> expressing BpFae<sup>T132C−D143C</sup> as post-induction temperature, pre-induction period, and post-induction period. Finally, we used the steepest ascent path design and response surface method to optimize the levels of these three factors further. Under the optimal conditions, the activity of BpFae<sup>T132C−D143C</sup> was 3.58 U/ml, which was a significant 6.6-fold increase compared to the pre-optimization (0.47 U/ml), demonstrating the effectiveness of this optimization process. Moreover, BpFae<sup>T132C−D143C</sup> activity was 1.52 U/ml in a 3-l fermenter under the abovementioned optimal conditions. It was determined that the expression of BpFae<sup>T132C−D143C</sup> in <i>E. coli</i> was predominantly intracellular in the cytoplasm. This study lays the foundation for further research on BpFae<sup>T132C−D143C</sup> in degrading agricultural waste transformation applications.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":"119 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142264819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biopriming with multifarious sulphur-oxidizing bacteria improve in vitro Vigna radiata L. (mung bean) and Brassica juncea L. (mustard) seed germination. 用多种硫氧化细菌进行生物处理可提高绿豆和芥菜种子的体外萌发。
IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-09-05 DOI: 10.1007/s12223-024-01195-8
Nandni, Savita Rani, Indu Dhiman, Leela Wati

Biopriming seeds with beneficial bacteria has potential to enhance seed germination. Therefore, in this investigation, five sulphur-oxidizing bacterial cultures, viz., Pantoea dispersa SOB2, Bacillus velezensis SN06, Bacillus cereus SN20, Bacillus tropicus SN16, and Bacillus megaterium SN11, were evaluated for different plant growth-promoting traits and their impact on Vigna radiata L. (mung bean) and Brassica juncea L. (mustard) seed germination. Among these, three bacterial cultures Pantoea dispersa SOB2, Bacillus velezensis SN06, and Bacillus megaterium SN11 evinced potential for mineral solubilization on solid medium where Pantoea dispersa SOB2 had the maximum solubilization indices-3.06, 5.14, and 2.48 for phosphate, zinc, and potassium respectively. The culture also displayed higher indole acetic acid (113.12 µg/mL), gibberellic acid (162.66 µg/mL), ammonia (5.23 µg/mL), and siderophore (69.53%) production than other bacterial cultures whereas Bacillus cereus SN20 showed maximum exopolysaccharide production (9.26 g/L). Bacterial culture Pantoea dispersa SOB2 significantly ameliorated the germination rate (3.73 no./day) and relative seed germination (208%) of Brassica juncea L., while Bacillus velezensis SN06 and Bacillus cereus SN20 followed with germination rate and relative seed germination of 2.86 no./day and 207%, respectively. Pantoea dispersa SOB2 displayed lowest mean germination time 2.91 days followed by Bacillus megaterium SN11 with 3.19 days. Biopriming with sulphur-oxidizing bacterial cultures, germination parameters of Vigna radiata L. were also markedly improved. Pantoea dispersa SOB2 demonstrated the highest germination rate (6.72 no./day), relative seed germination (115.56%), and minimum mean generation time (1.73 days). Bacillus velezensis SN06 inoculation had a beneficial effect on the seedling growth of Vigna radiata L., whereas Pantoea dispersa SOB2 greatly aided the seedling growth of Brassica juncea L. Results corroborated a prominent positive correlation between seed germination and plant growth-promoting traits. This is the first study on Pantoea dispersa as sulphur oxidizer, displaying plant growth promoting traits and seed germination potential. The potent sulphur-oxidizing bacterial cultures possessing plant growth promoting activities enhanced seed germination under in vitro conditions that could be further explored in field as biofertilizers to enhance the growth and yield of Brassica juncea L. and Vigna radiata L. crop.

用有益菌对种子进行生物处理有可能提高种子的萌发率。因此,在这项研究中,对五种硫氧化细菌培养物(即 Pantoea dispersa SOB2、Bacillus velezensis SN06、Bacillus cereus SN20、Bacillus tropicus SN16 和 Bacillus megaterium SN11)的不同植物生长促进特性及其对绿豆和芥菜种子萌发的影响进行了评估。其中,Pantoea dispersa SOB2、Bacillus velezensis SN06 和 Bacillus megaterium SN11 三种细菌培养物在固体培养基上具有矿物质增溶潜力,其中 Pantoea dispersa SOB2 对磷酸盐、锌和钾的增溶指数最大,分别为 3.06、5.14 和 2.48。该培养物的吲哚乙酸(113.12 微克/毫升)、赤霉素(162.66 微克/毫升)、氨(5.23 微克/毫升)和苷元(69.53%)产量也高于其他细菌培养物,而蜡样芽孢杆菌 SN20 的外多糖产量(9.26 克/升)最高。细菌培养物 Pantoea dispersa SOB2 显著提高了芥蓝的发芽率(3.73 个/天)和相对种子发芽率(208%),而枯草芽孢杆菌 SN06 和蜡样芽孢杆菌 SN20 的发芽率和相对种子发芽率分别为 2.86 个/天和 207%。Pantoea dispersa SOB2 的平均发芽时间最短,为 2.91 天,其次是芽孢杆菌 SN11,为 3.19 天。用硫氧化细菌培养物进行生物riming,也明显改善了 Vigna radiata L. 的发芽参数。Pantoea dispersa SOB2 的发芽率最高(6.72 粒/天),相对种子发芽率最高(115.56%),平均世代时间最短(1.73 天)。接种枯草芽孢杆菌(Bacillus velezensis)SN06 对黑木耳(Vigna radiata L.)的幼苗生长有利,而散盘菌(Pantoea dispersa)SOB2 对甘蓝(Brassica juncea L.)的幼苗生长有很大帮助。这是首次研究散囊菌作为硫氧化剂,具有促进植物生长的特性和种子萌发潜力。具有促进植物生长活性的强效硫氧化细菌培养物在体外条件下提高了种子萌发率,可进一步在田间作为生物肥料进行开发,以提高芸苔属植物和黑木耳的生长和产量。
{"title":"Biopriming with multifarious sulphur-oxidizing bacteria improve in vitro Vigna radiata L. (mung bean) and Brassica juncea L. (mustard) seed germination.","authors":"Nandni, Savita Rani, Indu Dhiman, Leela Wati","doi":"10.1007/s12223-024-01195-8","DOIUrl":"https://doi.org/10.1007/s12223-024-01195-8","url":null,"abstract":"<p><p>Biopriming seeds with beneficial bacteria has potential to enhance seed germination. Therefore, in this investigation, five sulphur-oxidizing bacterial cultures, viz., Pantoea dispersa SOB2, Bacillus velezensis SN06, Bacillus cereus SN20, Bacillus tropicus SN16, and Bacillus megaterium SN11, were evaluated for different plant growth-promoting traits and their impact on Vigna radiata L. (mung bean) and Brassica juncea L. (mustard) seed germination. Among these, three bacterial cultures Pantoea dispersa SOB2, Bacillus velezensis SN06, and Bacillus megaterium SN11 evinced potential for mineral solubilization on solid medium where Pantoea dispersa SOB2 had the maximum solubilization indices-3.06, 5.14, and 2.48 for phosphate, zinc, and potassium respectively. The culture also displayed higher indole acetic acid (113.12 µg/mL), gibberellic acid (162.66 µg/mL), ammonia (5.23 µg/mL), and siderophore (69.53%) production than other bacterial cultures whereas Bacillus cereus SN20 showed maximum exopolysaccharide production (9.26 g/L). Bacterial culture Pantoea dispersa SOB2 significantly ameliorated the germination rate (3.73 no./day) and relative seed germination (208%) of Brassica juncea L., while Bacillus velezensis SN06 and Bacillus cereus SN20 followed with germination rate and relative seed germination of 2.86 no./day and 207%, respectively. Pantoea dispersa SOB2 displayed lowest mean germination time 2.91 days followed by Bacillus megaterium SN11 with 3.19 days. Biopriming with sulphur-oxidizing bacterial cultures, germination parameters of Vigna radiata L. were also markedly improved. Pantoea dispersa SOB2 demonstrated the highest germination rate (6.72 no./day), relative seed germination (115.56%), and minimum mean generation time (1.73 days). Bacillus velezensis SN06 inoculation had a beneficial effect on the seedling growth of Vigna radiata L., whereas Pantoea dispersa SOB2 greatly aided the seedling growth of Brassica juncea L. Results corroborated a prominent positive correlation between seed germination and plant growth-promoting traits. This is the first study on Pantoea dispersa as sulphur oxidizer, displaying plant growth promoting traits and seed germination potential. The potent sulphur-oxidizing bacterial cultures possessing plant growth promoting activities enhanced seed germination under in vitro conditions that could be further explored in field as biofertilizers to enhance the growth and yield of Brassica juncea L. and Vigna radiata L. crop.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142132284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Harnessing nature's defenders: unveiling the potential of microbial consortia for plant defense induction against Alternaria blight in cumin. 利用大自然的卫士:揭示微生物联合体在诱导植物防御小茴香交替疫病方面的潜力。
IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-30 DOI: 10.1007/s12223-024-01191-y
Devendra Singh, Kuldeep Singh Jadon, Aman Verma, Rajesh Kumar Kakani

Present study was aimed to develop an efficient microbial consortium for combating Alternaria blight disease in cumin. The research involved isolating biocontrol agents against Alternaria burnsii, characterizing their biocontrol and growth promotion traits, and assessing compatibility. A pot experiment was conducted during rabi season of 2022-2023 to evaluate the bioefficacy of four biocontrol agents (1F, 16B, 31B, and 223B) individually and in consortium, focusing on disease severity, plant growth promotion, and defense responses in cumin challenged with A. burnsii. Microbial isolates 1F, 16B, 31B, and 223B significantly inhibited A. burnsii growth in dual plate assays (~ 86%), displaying promising biocontrol and plant growth promotion activities. They were identified as Trichoderma afroharzianum 1F, Aneurinibacillus aneurinilyticus 16B, Pseudomonas lalkuanensis 31B, and Bacillus licheniformis 223B, respectively. The excellent compatibility was observed among all selected biocontrol agents. Cumin plants treated with consortia of 1F + 16B + 31B + 223B showed least percent disease index (32.47%) and highest percent disease control (64.87%). Consortia of biocontrol agents significantly enhanced production of secondary metabolites (total phenol, flavonoids, antioxidant, and tannin) and activation of antioxidant-defense enzymes (POX, PPOX, CAT, SOD, PAL, and TAL) compared to individual biocontrol treatment and infected control. Moreover, consortium treatments effectively reduced electrolyte leakage over the individual biocontrol agent and infected control treatment. The four-microbe consortium significantly enhanced chlorophyll (154%), carotenoid content (88%), plant height (78.77%), dry weight (72.81%), and seed yield (104%) compared to infected control. Based on these findings, this environmentally friendly four-microbe consortium may be recommended for managing Alternaria blight in cumin.

本研究旨在开发一种高效的微生物联合体,用于防治小茴香的交替疫病。研究内容包括分离生物控制剂,鉴定其生物控制和生长促进特性,以及评估兼容性。在 2022-2023 年的蕾期进行了一项盆栽实验,以评估四种生物控制剂(1F、16B、31B 和 223B)单独和组合的生物功效,重点关注小茴香受燃烧疫霉菌侵染后的病害严重程度、植物生长促进作用和防御反应。在双平板试验中,微生物分离物 1F、16B、31B 和 223B 显著抑制了 A. burnsii 的生长(约 86%),显示出良好的生物防治和植物生长促进活性。经鉴定,它们分别是非洲毛霉 1F、动脉炎杆菌 16B、拉宽假单胞菌 31B 和地衣芽孢杆菌 223B。所有选定的生物控制剂之间都具有极好的兼容性。用 1F + 16B + 31B + 223B 联合体处理的小茴香植株病害指数(32.47%)最小,病害控制率(64.87%)最高。与单个生物防治处理和受感染的对照组相比,生物防治剂联合体显著提高了次生代谢物(总酚、类黄酮、抗氧化剂和单宁)的产量和抗氧化防御酶(POX、PPOX、CAT、SOD、PAL 和 TAL)的活化。此外,与单个生物控制剂和受感染的对照组相比,联合处理能有效减少电解质渗漏。与受感染的对照组相比,四微生物联合菌群可显著提高叶绿素(154%)、类胡萝卜素含量(88%)、株高(78.77%)、干重(72.81%)和种子产量(104%)。基于这些研究结果,可以推荐使用这种环境友好型四微生物联合体来管理小茴香的交替丝核菌枯萎病。
{"title":"Harnessing nature's defenders: unveiling the potential of microbial consortia for plant defense induction against Alternaria blight in cumin.","authors":"Devendra Singh, Kuldeep Singh Jadon, Aman Verma, Rajesh Kumar Kakani","doi":"10.1007/s12223-024-01191-y","DOIUrl":"https://doi.org/10.1007/s12223-024-01191-y","url":null,"abstract":"<p><p>Present study was aimed to develop an efficient microbial consortium for combating Alternaria blight disease in cumin. The research involved isolating biocontrol agents against Alternaria burnsii, characterizing their biocontrol and growth promotion traits, and assessing compatibility. A pot experiment was conducted during rabi season of 2022-2023 to evaluate the bioefficacy of four biocontrol agents (1F, 16B, 31B, and 223B) individually and in consortium, focusing on disease severity, plant growth promotion, and defense responses in cumin challenged with A. burnsii. Microbial isolates 1F, 16B, 31B, and 223B significantly inhibited A. burnsii growth in dual plate assays (~ 86%), displaying promising biocontrol and plant growth promotion activities. They were identified as Trichoderma afroharzianum 1F, Aneurinibacillus aneurinilyticus 16B, Pseudomonas lalkuanensis 31B, and Bacillus licheniformis 223B, respectively. The excellent compatibility was observed among all selected biocontrol agents. Cumin plants treated with consortia of 1F + 16B + 31B + 223B showed least percent disease index (32.47%) and highest percent disease control (64.87%). Consortia of biocontrol agents significantly enhanced production of secondary metabolites (total phenol, flavonoids, antioxidant, and tannin) and activation of antioxidant-defense enzymes (POX, PPOX, CAT, SOD, PAL, and TAL) compared to individual biocontrol treatment and infected control. Moreover, consortium treatments effectively reduced electrolyte leakage over the individual biocontrol agent and infected control treatment. The four-microbe consortium significantly enhanced chlorophyll (154%), carotenoid content (88%), plant height (78.77%), dry weight (72.81%), and seed yield (104%) compared to infected control. Based on these findings, this environmentally friendly four-microbe consortium may be recommended for managing Alternaria blight in cumin.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142105983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular identification of lactic acid bacteria from traditional fermented foods and screening exopolysaccharide production by using food wastes. 传统发酵食品中乳酸菌的分子鉴定及利用食品废弃物筛选外多糖的生产。
IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-27 DOI: 10.1007/s12223-024-01187-8
Kevser Karaman, Sibel Turan Sirke, Şeyda Nur Türkay Rifaioglu

In this study, lactic acid bacteria (LAB) isolation from fermented foods and molecular identification using magnetic bead technology were performed. And then exopolysaccharide (EPS) production possibility was tested in agar medium, and the positive ones were selected for the next step. The bacteria that could produce higher carbohydrate level were grown in MRS medium fortified with whey and pumpkin waste. In our study, 19 different LAB species were identified from fermented products collected from different places in Hatay (Türkiye) province. In molecular identification, universal primer pairs, p806R/p8FPL, and PEU7/DG74 were used for PCR amplification. After that, PCR products purified using paramagnetic bead technology were sequenced by the Sanger sequencing method. The dominant species, 23.8% of the isolates, were identified as Lactiplantibacillus plantarum. As a technological property of LAB, exopolysaccharide production capability of forty-two LAB isolate was tested in agar medium, and after eleven isolates were selected as positive. Two LAB (Latilactobacillus curvatus SHA2-3B and Loigolactobacillus coryniformis SHA6-3B) had higher EPS production capability when they were grown in MRS broth fortified with pumpkin waste and whey. The highest EPS content (1750 mg/L glucose equivalent) was determined in Loigolactobacillus coryniformis SHA6-3B grown in MRS broth fortified with 10% pumpkin waste. Besides the produced EPS samples were validated with FTIR and SEM methods.

本研究从发酵食品中分离出乳酸菌(LAB),并利用磁珠技术进行分子鉴定。然后在琼脂培养基中检测外多糖(EPS)产生的可能性,选出阳性菌进行下一步研究。在添加了乳清和南瓜废料的 MRS 培养基中培养能产生较高碳水化合物水平的细菌。在我们的研究中,从哈塔伊(图尔基耶)省不同地方收集的发酵产品中鉴定出了 19 种不同的 LAB 菌。在分子鉴定中,使用通用引物对 p806R/p8FPL 和 PEU7/DG74 进行 PCR 扩增。之后,利用顺磁珠技术纯化的 PCR 产物被桑格测序法测序。经鉴定,植物乳杆菌(Lactiplantibacillus plantarum)是主要的分离菌种,占 23.8%。作为 LAB 的一种技术特性,在琼脂培养基中对 42 株 LAB 分离物的外多糖生产能力进行了检测,结果有 11 株分离物被选中为阳性。在添加了南瓜废料和乳清的 MRS 肉汤中培养的两株 LAB(卷曲拉特兰乳杆菌 SHA2-3B 和褐藻乳杆菌 SHA6-3B)具有较高的 EPS 生产能力。在添加了 10%南瓜废料的 MRS 肉汤中生长的库里形塬乳杆菌 SHA6-3B 的 EPS 含量最高(1750 毫克/升葡萄糖当量)。此外,还用傅立叶变换红外光谱和扫描电镜方法对所生产的 EPS 样品进行了验证。
{"title":"Molecular identification of lactic acid bacteria from traditional fermented foods and screening exopolysaccharide production by using food wastes.","authors":"Kevser Karaman, Sibel Turan Sirke, Şeyda Nur Türkay Rifaioglu","doi":"10.1007/s12223-024-01187-8","DOIUrl":"https://doi.org/10.1007/s12223-024-01187-8","url":null,"abstract":"<p><p>In this study, lactic acid bacteria (LAB) isolation from fermented foods and molecular identification using magnetic bead technology were performed. And then exopolysaccharide (EPS) production possibility was tested in agar medium, and the positive ones were selected for the next step. The bacteria that could produce higher carbohydrate level were grown in MRS medium fortified with whey and pumpkin waste. In our study, 19 different LAB species were identified from fermented products collected from different places in Hatay (Türkiye) province. In molecular identification, universal primer pairs, p806R/p8FPL, and PEU7/DG74 were used for PCR amplification. After that, PCR products purified using paramagnetic bead technology were sequenced by the Sanger sequencing method. The dominant species, 23.8% of the isolates, were identified as Lactiplantibacillus plantarum. As a technological property of LAB, exopolysaccharide production capability of forty-two LAB isolate was tested in agar medium, and after eleven isolates were selected as positive. Two LAB (Latilactobacillus curvatus SHA2-3B and Loigolactobacillus coryniformis SHA6-3B) had higher EPS production capability when they were grown in MRS broth fortified with pumpkin waste and whey. The highest EPS content (1750 mg/L glucose equivalent) was determined in Loigolactobacillus coryniformis SHA6-3B grown in MRS broth fortified with 10% pumpkin waste. Besides the produced EPS samples were validated with FTIR and SEM methods.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142079884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Investigation of potential ascorbate peroxidase inhibitors for anti-leishmaniasis therapy. 研究潜在的抗坏血酸过氧化物酶抑制剂用于抗利什曼病治疗。
IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-17 DOI: 10.1007/s12223-024-01190-z
Ali A Rabaan, Mohammed Abdulrahman Alshahrani, Basim Othman, Mubarak Alfaresi, Yahya A Almutawif, Hamza M A Eid, Ahmed Saif, Ahmad A Alshehri

L eishmaniasis is a prevalent disease that impacts 98 countries and territories, mainly in Africa, Asia, and South America. It can cause substantial illness and death, particularly in its visceral manifestation that can be specifically targeted in the development of medications to combat leishmaniasis. This study has found natural compounds with possible inhibitory activity against APX using a reliable and accurate QSAR model. Despite the severe side effects of current treatments and the absence of an effective vaccination, these compounds show promise as a potential treatment for the disease. Nine hit compounds were found, and subsequent molecular docking was performed. Estradiol cypionate showed the lowest binding energy (- 10.5 kcal/mol), thus showing the strongest binding, and also had the strongest binding affinity, with a ΔGTotal of - 26.31 ± 3.01 kcal/mol, second only to the control molecule. Additionally, three hits viz. cloxacillin-sodium (- 16.57 ± 2.89 kcal/mol), cinchonidine (- 16.04 ± 3.27 kcal/mol), and quinine hydrochloride dihydrate (13.38 ± 1.06 kcal/mol) also showed significant binding affinity. Multiple interactions between drugs and active site residues demonstrated a substantial binding affinity with the target protein. The identified compounds exhibited drug-like effects and were orally bioavailable based on their ADME-toxicology features. Overall, estradiol cypionate, cloxacillin sodium, cinchonidine, and quinine hydrochloride dihydrate all exhibited inhibitory effects on the APX enzyme of Leishmania donovani. These results suggest that further investigation is needed to explore the potential of developing novel anti-leishmaniasis drugs using these compounds.

利什曼病是一种流行病,影响 98 个国家和地区,主要分布在非洲、亚洲和南美洲。利什曼病可导致严重的疾病和死亡,尤其是其内脏表现,在开发抗击利什曼病的药物时可专门针对这种疾病。这项研究利用可靠、准确的 QSAR 模型发现了可能对 APX 具有抑制活性的天然化合物。尽管目前的治疗方法有严重的副作用,也没有有效的疫苗,但这些化合物有望成为治疗该疾病的潜在药物。研究发现了九种命中化合物,并进行了后续的分子对接。环戊丙酸雌二醇的结合能最低(- 10.5 kcal/mol),因此结合力最强,结合亲和力也最强,ΔGTotal 为 - 26.31 ± 3.01 kcal/mol,仅次于对照分子。此外,氯唑西林钠(- 16.57 ± 2.89 kcal/mol)、辛可尼丁(- 16.04 ± 3.27 kcal/mol)和盐酸奎宁二水合物(13.38 ± 1.06 kcal/mol)这三种药物也显示出显著的结合亲和力。药物与活性位点残基之间的多重相互作用表明,药物与目标蛋白质具有很强的结合亲和力。根据其 ADME 毒理学特征,鉴定出的化合物表现出类似药物的效果,并具有口服生物利用度。总的来说,环戊丙酸雌二醇、氯唑西林钠、辛可尼丁和奎宁盐酸盐二水合物都对唐氏利什曼原虫的 APX 酶有抑制作用。这些结果表明,利用这些化合物开发新型抗利什曼病药物的潜力有待进一步研究。
{"title":"Investigation of potential ascorbate peroxidase inhibitors for anti-leishmaniasis therapy.","authors":"Ali A Rabaan, Mohammed Abdulrahman Alshahrani, Basim Othman, Mubarak Alfaresi, Yahya A Almutawif, Hamza M A Eid, Ahmed Saif, Ahmad A Alshehri","doi":"10.1007/s12223-024-01190-z","DOIUrl":"https://doi.org/10.1007/s12223-024-01190-z","url":null,"abstract":"<p><p>L eishmaniasis is a prevalent disease that impacts 98 countries and territories, mainly in Africa, Asia, and South America. It can cause substantial illness and death, particularly in its visceral manifestation that can be specifically targeted in the development of medications to combat leishmaniasis. This study has found natural compounds with possible inhibitory activity against APX using a reliable and accurate QSAR model. Despite the severe side effects of current treatments and the absence of an effective vaccination, these compounds show promise as a potential treatment for the disease. Nine hit compounds were found, and subsequent molecular docking was performed. Estradiol cypionate showed the lowest binding energy (- 10.5 kcal/mol), thus showing the strongest binding, and also had the strongest binding affinity, with a ΔG<sub>Total</sub> of - 26.31 ± 3.01 kcal/mol, second only to the control molecule. Additionally, three hits viz. cloxacillin-sodium (- 16.57 ± 2.89 kcal/mol), cinchonidine (- 16.04 ± 3.27 kcal/mol), and quinine hydrochloride dihydrate (13.38 ± 1.06 kcal/mol) also showed significant binding affinity. Multiple interactions between drugs and active site residues demonstrated a substantial binding affinity with the target protein. The identified compounds exhibited drug-like effects and were orally bioavailable based on their ADME-toxicology features. Overall, estradiol cypionate, cloxacillin sodium, cinchonidine, and quinine hydrochloride dihydrate all exhibited inhibitory effects on the APX enzyme of Leishmania donovani. These results suggest that further investigation is needed to explore the potential of developing novel anti-leishmaniasis drugs using these compounds.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141995573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Combinatorial application of cuminaldehyde and gentamicin shows enhanced antimicrobial and antibiofilm action on Pseudomonas aeruginosa. 积雪苷和庆大霉素的联合应用增强了对铜绿假单胞菌的抗菌和抗生物膜作用。
IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-01 Epub Date: 2023-12-22 DOI: 10.1007/s12223-023-01121-4
Sudipta Chatterjee, Payel Paul, Poulomi Chakraborty, Sharmistha Das, Anirban Das Gupta, Ritwik Roy, Moumita Malik, Sarita Sarkar, Ranojit Kumar Sarker, Prosun Tribedi

The emergence of biofilm-induced drug tolerance poses a critical challenge to public healthcare management. Pseudomonas aeruginosa, a gram-negative opportunistic bacterium, is involved in various biofilm-associated infections in human hosts. Towards this direction, in the present study, a combinatorial approach has been explored as it is a demonstrably effective strategy for managing microbial infections. Thus, P. aeruginosa has been treated with cuminaldehyde (a naturally occurring phytochemical) and gentamicin (an aminoglycoside antibiotic) in connection to the effective management of the biofilm challenges. It was also observed that the test molecules could show increased antimicrobial activity against P. aeruginosa. A fractional inhibitory concentration index (FICI) of 0.65 suggested an additive interaction between cuminaldehyde and gentamicin. Besides, a series of experiments such as crystal violet assay, estimation of extracellular polymeric substance (EPS), and microscopic images indicated that an enhanced antibiofilm activity was obtained when the selected compounds were applied together on P. aeruginosa. Furthermore, the combination of the selected compounds was found to reduce the secretion of virulence factors from P. aeruginosa. Taken together, this study suggested that the combinatorial application of cuminaldehyde and gentamicin could be considered an effective approach towards the control of biofilm-linked infections caused by P. aeruginosa.

生物膜诱导耐药性的出现给公共医疗保健管理带来了严峻的挑战。铜绿假单胞菌是一种革兰氏阴性机会性细菌,它参与了人类宿主的各种生物膜相关感染。为此,本研究探索了一种组合方法,因为它是管理微生物感染的一种明显有效的策略。因此,用积雪苷(一种天然植物化学物质)和庆大霉素(一种氨基糖苷类抗生素)处理铜绿假单胞菌,以有效控制生物膜挑战。研究还发现,测试分子对铜绿假单胞菌具有更强的抗菌活性。部分抑制浓度指数(FICI)为 0.65,表明积雪苷和庆大霉素之间存在相加作用。此外,一系列实验,如水晶紫测定法、细胞外聚合物质(EPS)估算法和显微图像显示,当所选化合物同时应用于铜绿假单胞菌时,其抗生物膜活性得到了增强。此外,研究还发现所选化合物的组合能减少铜绿假单胞菌分泌毒力因子。综上所述,这项研究表明,积雪苷和庆大霉素的组合应用可被视为控制铜绿假单胞菌引起的生物膜相关感染的一种有效方法。
{"title":"Combinatorial application of cuminaldehyde and gentamicin shows enhanced antimicrobial and antibiofilm action on Pseudomonas aeruginosa.","authors":"Sudipta Chatterjee, Payel Paul, Poulomi Chakraborty, Sharmistha Das, Anirban Das Gupta, Ritwik Roy, Moumita Malik, Sarita Sarkar, Ranojit Kumar Sarker, Prosun Tribedi","doi":"10.1007/s12223-023-01121-4","DOIUrl":"10.1007/s12223-023-01121-4","url":null,"abstract":"<p><p>The emergence of biofilm-induced drug tolerance poses a critical challenge to public healthcare management. Pseudomonas aeruginosa, a gram-negative opportunistic bacterium, is involved in various biofilm-associated infections in human hosts. Towards this direction, in the present study, a combinatorial approach has been explored as it is a demonstrably effective strategy for managing microbial infections. Thus, P. aeruginosa has been treated with cuminaldehyde (a naturally occurring phytochemical) and gentamicin (an aminoglycoside antibiotic) in connection to the effective management of the biofilm challenges. It was also observed that the test molecules could show increased antimicrobial activity against P. aeruginosa. A fractional inhibitory concentration index (FICI) of 0.65 suggested an additive interaction between cuminaldehyde and gentamicin. Besides, a series of experiments such as crystal violet assay, estimation of extracellular polymeric substance (EPS), and microscopic images indicated that an enhanced antibiofilm activity was obtained when the selected compounds were applied together on P. aeruginosa. Furthermore, the combination of the selected compounds was found to reduce the secretion of virulence factors from P. aeruginosa. Taken together, this study suggested that the combinatorial application of cuminaldehyde and gentamicin could be considered an effective approach towards the control of biofilm-linked infections caused by P. aeruginosa.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":"823-834"},"PeriodicalIF":2.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138829140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Newly isolated Brevundimonas naejangsanensis as a biocontrol agent against Fusarium redolens the causal of Fusarium yellows of chickpea. 新分离的 Brevundimonas naejangsanensis 作为一种生物控制剂,可防治鹰嘴豆黄化镰刀菌的病原 Fusarium redolens。
IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-01 Epub Date: 2024-01-04 DOI: 10.1007/s12223-023-01126-z
Ahmed Amine Bekkar, Souad Zaim

Three endophytic bacteria, namely BvV, BvP and BvL, were newly isolated from the root nodules of bean, pea and lentil plants respectively cultivated in Mascara the northwest of Algeria, and identified by 16S ribosomal RNA gene sequencing as Brevundimonas naejangsanensis. These strains were able to produce hydrolytic enzymes and hydrogen cyanide. All strains produced a growth-promoting hormone, indole acetic acid, varying in concentration from 83.2 to 171.7 µg/mL. The phosphate solubilizing activity of BvV, BvP and BvL varied from 25.5 to 42.02 µg/mL for tricalcium phosphate. The three antagonistic Brevundimonas spp. showed in vitro the most inhibitory effect on mycelial growth of Fusarium redolens FRC (from 78.33 to 85.55%). Strain BvV, BvP and BvL produced also volatile metabolites which inhibited mycelial FRC growth up to 39.2%. All strains showed significant disease reduction in pot experiments. Chickpea Fusarium yellows severity caused by FRC was reduced significantly from 89.3 to 96.6% in the susceptible cultivar ILC 482 treated with antagonistic B. naejangsanensis. The maximum stimulatory effect on chickpea plants growth was observed by inoculation of strain BvV. This treatment resulted in a 7.40-26.21% increase in shoot height as compared to the control plants. It is concluded that the endophytic bacterial strains of B. naejangsanensis having different plant growth promoting (PGP) activities can be considered as beneficial microbes for sustainable agriculture. To our knowledge, this is the first report to use B. naejangsanensis strains as a new biocontrol agent against F. redolens, a new pathogen of chickpea plants causing Fusarium yellows disease in Algeria.

通过 16S 核糖体 RNA 基因测序,从阿尔及利亚西北部 Mascara 地区栽培的蚕豆、豌豆和扁豆植物的根瘤中新分离出三种内生细菌,即 BvV、BvP 和 BvL,并确定它们为 Brevundimonas naejangsanensis。这些菌株能够产生水解酶和氰化氢。所有菌株都能产生一种促进生长的激素--吲哚乙酸,浓度从 83.2 微克/毫升到 171.7 微克/毫升不等。BvV、BvP 和 BvL 对磷酸三钙的磷酸盐溶解活性从 25.5 微克/毫升到 42.02 微克/毫升不等。三种拮抗剂 Brevundimonas 菌属在体外对 Fusarium redolens FRC 菌丝生长的抑制作用最大(从 78.33% 到 85.55%)。菌株 BvV、BvP 和 BvL 还产生挥发性代谢物,对 FRC 菌丝生长的抑制率高达 39.2%。在盆栽实验中,所有菌株都能明显减轻病害。用拮抗剂 B. naejangsanensis 处理易感栽培品种 ILC 482,由 FRC 引起的鹰嘴豆镰刀菌黄化病严重程度从 89.3% 明显降低到 96.6%。接种菌株 BvV 对鹰嘴豆植株生长的刺激作用最大。与对照植物相比,这种处理方法使芽高增加了 7.40-26.21%。结论是,具有不同植物生长促进(PGP)活性的 B. naejangsanensis 内生细菌菌株可被视为可持续农业的有益微生物。据我们所知,这是第一份利用 B. naejangsanensis 菌株作为新的生物控制剂来防治 F. redolens 的报告,F. redolens 是导致阿尔及利亚鹰嘴豆黄化病的一种新病原体。
{"title":"Newly isolated Brevundimonas naejangsanensis as a biocontrol agent against Fusarium redolens the causal of Fusarium yellows of chickpea.","authors":"Ahmed Amine Bekkar, Souad Zaim","doi":"10.1007/s12223-023-01126-z","DOIUrl":"10.1007/s12223-023-01126-z","url":null,"abstract":"<p><p>Three endophytic bacteria, namely BvV, BvP and BvL, were newly isolated from the root nodules of bean, pea and lentil plants respectively cultivated in Mascara the northwest of Algeria, and identified by 16S ribosomal RNA gene sequencing as Brevundimonas naejangsanensis. These strains were able to produce hydrolytic enzymes and hydrogen cyanide. All strains produced a growth-promoting hormone, indole acetic acid, varying in concentration from 83.2 to 171.7 µg/mL. The phosphate solubilizing activity of BvV, BvP and BvL varied from 25.5 to 42.02 µg/mL for tricalcium phosphate. The three antagonistic Brevundimonas spp. showed in vitro the most inhibitory effect on mycelial growth of Fusarium redolens FRC (from 78.33 to 85.55%). Strain BvV, BvP and BvL produced also volatile metabolites which inhibited mycelial FRC growth up to 39.2%. All strains showed significant disease reduction in pot experiments. Chickpea Fusarium yellows severity caused by FRC was reduced significantly from 89.3 to 96.6% in the susceptible cultivar ILC 482 treated with antagonistic B. naejangsanensis. The maximum stimulatory effect on chickpea plants growth was observed by inoculation of strain BvV. This treatment resulted in a 7.40-26.21% increase in shoot height as compared to the control plants. It is concluded that the endophytic bacterial strains of B. naejangsanensis having different plant growth promoting (PGP) activities can be considered as beneficial microbes for sustainable agriculture. To our knowledge, this is the first report to use B. naejangsanensis strains as a new biocontrol agent against F. redolens, a new pathogen of chickpea plants causing Fusarium yellows disease in Algeria.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":"835-846"},"PeriodicalIF":2.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139086554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antioxidant properties and L-asparaginase activities of endophytic fungi from Cymbidium orchids. 兰花内生真菌抗氧化性能及l -天冬酰胺酶活性研究。
IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-01 Epub Date: 2023-11-23 DOI: 10.1007/s12223-023-01112-5
Ru Wei Chua, Keang Peng Song, Adeline Su Yien Ting

This study profiled the various endophytic fungi isolated from the orchid Cymbidium sp. and their L-asparaginase production and antioxidant potential. The L-asparaginase production was first screened through qualitative plate screening then quantified by the Nesslerization method. The antioxidant potential was quantified via the 2,2-diphenyl-1-picrylhydrazyl assay. A total of 30 endophytic fungi were isolated and all fungal isolates exhibited various degrees of radical scavenging activities (45.28% to 76.4%). Isolate Lasiodiplodia theobromae (C11) had the highest antioxidant capacity, represented by the lowest IC50 value (5.75 mg/mL) and highest ascorbic acid equivalent antioxidant capacity value (12.17 mg/g). Additionally, 16 isolates produced L-asparaginase (53.33%), which includes primarily species of Fusarium proliferatum, Fusarium fujikuroi, Fusarium incarnatum, and Fusarium oxysporum. A new isolate has also been discovered from Cymbidium orchid, Buergenerula spartinae (C28), which showed the highest L-asparaginase activity (1.736 unit/mL). These findings supported the postulation that medicinal species of Orchidaceae such as Cymbidium sp. harbor endophytes that are producers of L-asparaginase and antioxidants with various potential applications.

本文研究了从兰花中分离的各种内生真菌及其l -天冬酰胺酶的产生和抗氧化能力。首先通过定性平板筛选筛选l -天冬酰胺酶的产量,然后用neslerization法定量。通过2,2-二苯基-1-苦味酰肼测定法测定其抗氧化能力。共分离到30株内生真菌,均表现出不同程度的自由基清除能力(45.28% ~ 76.4%)。分离物可可叶(C11)抗氧化能力最强,IC50值最低(5.75 mg/mL),抗坏血酸当量抗氧化能力最高(12.17 mg/g)。另外,产生l -天冬酰胺酶的菌株有16株(53.33%),主要包括增肉镰刀菌、藤黑镰刀菌、incarnatum镰刀菌和oxysporum镰刀菌。从蕙兰(Cymbidium orchid)中也发现了一株新分离物Buergenerula spartinae (C28),其l -天冬酰胺酶活性最高,为1.736单位/mL。这些发现支持了兰科药用植物如蕙兰(Cymbidium sp.)中含有内生菌的假设,这些内生菌是l -天冬酰胺酶和抗氧化剂的制造者,具有多种潜在的应用前景。
{"title":"Antioxidant properties and L-asparaginase activities of endophytic fungi from Cymbidium orchids.","authors":"Ru Wei Chua, Keang Peng Song, Adeline Su Yien Ting","doi":"10.1007/s12223-023-01112-5","DOIUrl":"10.1007/s12223-023-01112-5","url":null,"abstract":"<p><p>This study profiled the various endophytic fungi isolated from the orchid Cymbidium sp. and their L-asparaginase production and antioxidant potential. The L-asparaginase production was first screened through qualitative plate screening then quantified by the Nesslerization method. The antioxidant potential was quantified via the 2,2-diphenyl-1-picrylhydrazyl assay. A total of 30 endophytic fungi were isolated and all fungal isolates exhibited various degrees of radical scavenging activities (45.28% to 76.4%). Isolate Lasiodiplodia theobromae (C11) had the highest antioxidant capacity, represented by the lowest IC<sub>50</sub> value (5.75 mg/mL) and highest ascorbic acid equivalent antioxidant capacity value (12.17 mg/g). Additionally, 16 isolates produced L-asparaginase (53.33%), which includes primarily species of Fusarium proliferatum, Fusarium fujikuroi, Fusarium incarnatum, and Fusarium oxysporum. A new isolate has also been discovered from Cymbidium orchid, Buergenerula spartinae (C28), which showed the highest L-asparaginase activity (1.736 unit/mL). These findings supported the postulation that medicinal species of Orchidaceae such as Cymbidium sp. harbor endophytes that are producers of L-asparaginase and antioxidants with various potential applications.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":"713-722"},"PeriodicalIF":2.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138295041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A recombinase polymerase amplification-SYBR Green I assay for the rapid and visual detection of Brucella. 一种重组酶聚合酶扩增- sybr Green I检测布鲁氏菌的方法。
IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-01 Epub Date: 2023-12-02 DOI: 10.1007/s12223-023-01115-2
Jiang Chang, Nan Wang, Jun-Peng Zhan, Shi-Jun Zhang, De-Ying Zou, Feng Li, Ying Zhang, Yan-Song Li, Pan Hu, Shi-Ying Lu, Zeng-Shan Liu, Hong-Lin Ren

Brucellosis is a zoonosis caused by Brucella, which poses a great threat to human health and animal husbandry. Pathogen surveillance is an important measure to prevent brucellosis, but the traditional method is time-consuming and not suitable for field applications. In this study, a recombinase polymerase amplification-SYBR Green I (RPAS) assay was developed for the rapid and visualized detection of Brucella in the field by targeting BCSP31 gene, a conserved marker. The method was highly specific without any cross-reactivity with other common bacteria and its detection limit was 2.14 × 104 CFU/mL or g of Brucella at 40 °C for 20 min. It obviates the need for costly instrumentation and exhibits robustness towards background interference in serum, meat, and milk samples. In summary, the RPAS assay is a rapid, visually intuitive, and user-friendly detection that is highly suitable for use in resource-limited settings. Its simplicity and ease of use enable swift on-site detection of Brucella, thereby facilitating timely implementation of preventive measures.

布鲁氏菌病是由布鲁氏菌引起的人畜共患病,对人类健康和畜牧业造成极大威胁。病原菌监测是预防布鲁氏菌病的重要措施,但传统方法耗时长,不适合现场应用。本研究以布鲁氏菌的保守标记BCSP31基因为靶点,建立了一种重组酶聚合酶扩增- sybr Green I (RPAS)方法,用于布鲁氏菌的野外快速、可视化检测。该方法具有高度特异性,与其他常见细菌无交叉反应性,在40°C、20 min条件下,布鲁氏菌的检出限为2.14 × 104 CFU/mL或g。该方法不需要昂贵的仪器,对血清、肉类和牛奶样品的背景干扰具有鲁棒性。总之,RPAS分析法是一种快速、直观、用户友好的检测方法,非常适合在资源有限的环境中使用。它简单易用,能够在现场迅速检测布鲁氏菌,从而促进及时实施预防措施。
{"title":"A recombinase polymerase amplification-SYBR Green I assay for the rapid and visual detection of Brucella.","authors":"Jiang Chang, Nan Wang, Jun-Peng Zhan, Shi-Jun Zhang, De-Ying Zou, Feng Li, Ying Zhang, Yan-Song Li, Pan Hu, Shi-Ying Lu, Zeng-Shan Liu, Hong-Lin Ren","doi":"10.1007/s12223-023-01115-2","DOIUrl":"10.1007/s12223-023-01115-2","url":null,"abstract":"<p><p>Brucellosis is a zoonosis caused by Brucella, which poses a great threat to human health and animal husbandry. Pathogen surveillance is an important measure to prevent brucellosis, but the traditional method is time-consuming and not suitable for field applications. In this study, a recombinase polymerase amplification-SYBR Green I (RPAS) assay was developed for the rapid and visualized detection of Brucella in the field by targeting BCSP31 gene, a conserved marker. The method was highly specific without any cross-reactivity with other common bacteria and its detection limit was 2.14 × 10<sup>4</sup> CFU/mL or g of Brucella at 40 °C for 20 min. It obviates the need for costly instrumentation and exhibits robustness towards background interference in serum, meat, and milk samples. In summary, the RPAS assay is a rapid, visually intuitive, and user-friendly detection that is highly suitable for use in resource-limited settings. Its simplicity and ease of use enable swift on-site detection of Brucella, thereby facilitating timely implementation of preventive measures.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":"767-774"},"PeriodicalIF":2.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138470177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Folia microbiologica
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1