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Characteristics and in vitro properties of potential probiotic strain Fructobacillus tropaeoli KKP 3032 isolated from orange juice. 从橙汁中分离出的潜在益生菌株 Fructobacillus tropaeoli KKP 3032 的特征和体外特性。
IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-02-01 Epub Date: 2024-11-14 DOI: 10.1007/s12223-024-01207-7
Anna Mikołajczuk-Szczyrba, Adrian Wojtczak, Marek Kieliszek, Barbara Sokołowska

Fructobacillus, a Gram-positive, non-spore-forming, facultative anaerobic bacterium, belongs to the fructophilic lactic acid bacteria (FLAB) group. The group's name originates from fructose, the favored carbon source for its members. Fructobacillus spp. are noteworthy for their distinctive traits, captivating the interest of scientists. However, there have been relatively few publications regarding the isolation and potential utilization of these microorganisms in the industry. In recent years, F. tropaeoli has garnered interest for its promising role in the food and pharmaceutical sectors, although the availability of isolates is rather limited. A more comprehensive understanding of Fructobacillus is imperative to evaluate their functionality in the industry, given their unique and exceptional properties. Our in vitro study on Fructobacillus tropaeoli KKP 3032 confirmed its fructophilic nature and high osmotolerance. This strain thrives in a 30% sugar concentration, shows resistance to low pH and bile salts, and exhibits robust autoaggregation. Additionally, it displays significant antimicrobial activity against foodborne pathogens. Evaluating its probiotic potential, it aligns with EFSA recommendations in antibiotic resistance, except for kanamycin, to which it is resistant. Further research is necessary, but preliminary analyses confirm the high probiotic potential of F. tropaeoli KKP 3032 and its ability to thrive in the presence of high concentrations of fructose. The results indicate that the isolate F. tropaeoli KKP 3032 could potentially be used in the future as a fructophilic probiotic, protective culture, and/or active ingredient in fructose-rich food.

果酸杆菌(Fructobacillus)是一种革兰氏阳性、无芽孢、兼性厌氧细菌,属于嗜果乳酸菌(FLAB)类。该菌群的名称源于其成员最喜欢的碳源--果糖。果酸杆菌属因其独特的性状而备受关注,吸引着科学家们的兴趣。然而,有关这些微生物的分离和在工业中的潜在利用的出版物相对较少。近年来,F. tropaeoli 因其在食品和制药领域的巨大作用而备受关注,但其分离物却相当有限。鉴于其独特而非凡的特性,要评估其在工业中的功能,就必须更全面地了解果杆菌。我们对 Fructobacillus tropaeoli KKP 3032 的体外研究证实了它的嗜果性和高渗透耐受性。该菌株能在 30% 的糖浓度下茁壮成长,对低 pH 值和胆盐具有耐受性,并表现出强大的自身聚集能力。此外,它还对食源性病原体具有显著的抗菌活性。在评估其益生菌潜力时,它符合欧洲食品安全局关于抗生素耐药性的建议,但卡那霉素除外,因为它对卡那霉素具有耐药性。虽然还需要进一步研究,但初步分析证实了 F. tropaeoli KKP 3032 的高益生菌潜力及其在高浓度果糖条件下的生长能力。结果表明,F. tropaeoli KKP 3032 分离物将来有可能用作富含果糖的食品中的嗜果益生菌、保护性培养物和/或活性成分。
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引用次数: 0
Decolourization and detoxification of Reactive Red-195 azo dye by Staphylococcus caprae isolated from textile effluent. 从纺织废水中分离出的 Caprae 葡萄球菌对 Reactive Red-195 偶氮染料的脱醇和解毒作用。
IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-02-01 Epub Date: 2024-06-19 DOI: 10.1007/s12223-024-01175-y
Monika Yadav, Asha Lata Singh

Azo dyes are used as coloring agent in textile industries at larger scale. As a result, large quantity of dye-enriched waste water is generated which subsequently poses environmental problems. Biological tool involving bacteria having azoreductase enzyme has proved to be more effective and efficient in dye effluent treatment. Current work focuses on Staphylococcus caprae (S. caprae) for degradation and decolorization of Reactive Red-195 (RR-195) azo dye. For this purpose, factors such as pH, temperature, inoculums, carbon and nitrogen sources, and dye concentrations have been optimized for maximum decolorization and degradation. S. caprae (4 mg/mL) efficiently resulted into 90% decolorization of RR-195 dye under static condition at 100 µg/mL concentration, 30 °C and pH 7.0 at a 12-h contact period. FTIR analysis has revealed the formation of new functional groups in the treated dye such as O-H stretch at 3370 cm-1, C-H band stretching at 2928 cm-1, and new band at 1608 cm-1 which specify the degradation of aromatic ring, 1382 and 1118 cm-1 represents desulfonated peaks. Biodegraded metabolites of RR-195 dye such as phenol, 3, 5-di-tert-butylphenol, and phthalic acid have been identified respectively that find industrial applications. Phytotoxicity test has shown non-toxic effects of treated dye on germination of Vigna radiata and Triticum aestivum seeds. Further, antibiotic diffusion assay has confirmed the biosafety of S. caprae.

纺织业大量使用偶氮染料作为染色剂。因此,会产生大量富含染料的废水,进而造成环境问题。事实证明,利用含有偶氮还原酶的细菌进行生物处理,能更有效地处理染料废水。目前的工作重点是用 Caprae 葡萄球菌(S. caprae)降解和脱色 Reactive Red-195 (RR-195) 偶氮染料。为此,对 pH 值、温度、接种物、碳源和氮源以及染料浓度等因素进行了优化,以实现最大程度的脱色和降解。在浓度为 100 µg/mL、温度为 30 °C、pH 值为 7.0、接触时间为 12 小时的静态条件下,S. caprae(4 mg/mL)能有效地使 RR-195 染料脱色 90%。傅立叶变换红外光谱分析显示,处理后的染料中形成了新的官能团,如 3370 cm-1 的 O-H 伸展带、2928 cm-1 的 C-H 伸展带和 1608 cm-1 的新带,其中 1608 cm-1 代表芳香环的降解,1382 和 1118 cm-1 代表脱硫峰。经鉴定,RR-195 染料的生物降解代谢物(如苯酚、3, 5-二叔丁基苯酚和邻苯二甲酸)可用于工业。植物毒性测试表明,经处理的染料对 Vigna radiata 和 Triticum aestivum 种子的萌发无毒性影响。此外,抗生素扩散试验也证实了 S. caprae 的生物安全性。
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引用次数: 0
A rare case report of tissue infection caused by Pantoea piersonii (basionym Kalamiella piersonii). 一例罕见的由 piersonii 盘尾丝菌(别名 piersonii Kalamiella)引起的组织感染病例报告。
IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-02-01 Epub Date: 2024-10-04 DOI: 10.1007/s12223-024-01203-x
Kubra Atilan, Tayfun Ozdem, Canset Nur Aydogan, Tugrul Hosbul

In 2019, Pantoea piersonii was initially isolated from the interior surfaces of the International Space Station. This microorganism is a species within the genus Pantoea in the family Erwiniaceae, belonging to the order Enterobacterales. Recent literature has documented four cases of its isolation. Despite initial predictions suggesting the non-pathogenicity of P. piersonii strains, evidence from observed cases indicates potential pathogenicity. According to documented evidence in the literature, this microorganism is capable of causing severe and life-threatening conditions, including sepsis. Traditional tests, as well as automated systems, may fail to provide complete differentiation due to these similarities. While MALDI-TOF MS is a valuable tool for identification in clinical diagnostic microbiology, sequencing may be necessary for precise identification. To determine the antibiotic susceptibility profile, various methods can be utilized, including minimum inhibitory concentration determination, disk diffusion testing (Kirby-Bauer test), genotypic resistance assays (PCR and sequencing), and automated systems. The literature reports a limited number of cases associating P. piersonii with human infection. This study contributes to this body of knowledge by reporting a novel case in which P. piersonii was isolated from a tissue sample for the first time. In this case report, the patient achieved recovery following the administration of appropriate antibiotic treatment based on the diagnosis. It underscores the need for precise identification and understanding of its pathogenicity.

2019 年,最初从国际空间站的内部表面分离出 Pantoea piersonii。这种微生物是埃文菌科泛氏菌属中的一个物种,属于肠杆菌目。最近的文献记录了四例分离到它的病例。尽管最初的预测表明 P. piersonii 菌株不具有致病性,但观察到的病例证据表明其具有潜在的致病性。根据文献记载的证据,这种微生物能够引起严重的危及生命的病症,包括败血症。由于这些相似性,传统检测方法和自动化系统可能无法完全区分。虽然 MALDI-TOF MS 是临床诊断微生物学鉴定的重要工具,但要精确鉴定可能还需要测序。要确定抗生素敏感性概况,可采用多种方法,包括最小抑菌浓度测定法、盘扩散试验(柯比-鲍尔试验)、基因型耐药性测定法(PCR 和测序)以及自动化系统。文献报道的皮尔森癣菌与人类感染相关的病例数量有限。本研究报告了一个新病例,首次从组织样本中分离出了皮尔森氏菌,为这一知识体系做出了贡献。在该病例报告中,根据诊断结果给予适当的抗生素治疗后,患者获得了康复。它强调了精确鉴定和了解其致病性的必要性。
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引用次数: 0
Asymptomatic carriage and molecular characterization of Staphylococcus aureus in pre-clinical and clinical medical students. 临床前及临床医学生金黄色葡萄球菌无症状携带及分子特征分析。
IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-02-01 Epub Date: 2025-01-13 DOI: 10.1007/s12223-024-01237-1
Kristýna Brodíková, Bohdana Rezková, Ivana Koláčková, Renáta Karpíšková

Medical students are exposed to the hospital environment and patients during their studies, increasing the risk of exposure to virulent and antibiotic-resistant isolates of Staphylococcus aureus. The aim of the study is to determine the prevalence of Staphylococcus aureus among medical students who have varying levels of exposure to the hospital environment to provide valuable insights into the risk of colonization and transmission. Nasal swabs and fingerprints were obtained and cultured on a selective medium for staphylococci. The obtained isolates were confirmed as methicillin-sensitive S. aureus (MSSA) or methicillin-resistant (MRSA) using PCR. Antibiotic resistance, the presence of virulence genes including enterotoxin encoding genes, and spa typing were performed. Among pre-clinical students, MSSA was detected on the nose in 45.2% and on the fingerprints in 10.6% of the participants. Among clinical students, MSSA was detected on the nose in 42.0% and on the fingerprints in 25.4%. Only one MRSA isolate was obtained. Genes seg and sei were the most frequently detected in both student groups, with their presence in over 40% of isolates among clinical students. The eta and etb genes were mainly detected from the nose in both student groups. In pre-clinical students, S. aureus carrying eta gene occurred in 6.4% and etb in 8.5%. In clinical students, the occurrence was 5.1% for eta and 8.5% for etb. The tst gene was identified only in the nose and fingerprints of the clinical student group. The most frequently observed resistance was to clindamycin and erythromycin. In total 58 different spa types were identified. High rates of asymptomatic MSSA carriage were observed in both groups of medical students. Detected MSSA strains showed a high degree of genetic variability, with a number of them carrying the virulence and antibiotic resistance genes. Although students do not exhibit increased risk to their patient's, increased hygiene is required in asymptomatic carriage personnel. The overall prevalence of MRSA was low, with a minimal risk of spread.

医学生在学习期间与医院环境和病人接触,增加了接触强毒性和耐抗生素金黄色葡萄球菌分离株的风险。本研究的目的是确定在不同程度暴露于医院环境的医学生中金黄色葡萄球菌的患病率,以提供对定植和传播风险的有价值的见解。获得鼻拭子和指纹,并在葡萄球菌选择性培养基上培养。采用PCR方法鉴定分离株为甲氧西林敏感型金黄色葡萄球菌(MSSA)或耐甲氧西林型金黄色葡萄球菌(MRSA)。进行了抗生素耐药性、毒力基因(包括肠毒素编码基因)的存在和spa分型。在临床预科学生中,45.2%的人在鼻子上检测到MSSA, 10.6%的人在指纹上检测到MSSA。在临床学生中,鼻检出率为42.0%,指纹检出率为25.4%。仅获得一株MRSA分离物。基因seg和sei在两组学生中最常被检测到,在临床学生中有超过40%的分离物存在它们。eta和etb基因主要在两组学生的鼻子中检测到。在临床前学生中,携带eta基因的金黄色葡萄球菌占6.4%,携带etb基因的金黄色葡萄球菌占8.5%。在临床学生中,eta和etb的发生率分别为5.1%和8.5%。该基因仅在临床学生组的鼻子和指纹中被鉴定出来。最常见的耐药是克林霉素和红霉素。总共确定了58种不同的水疗类型。在两组医学生中均观察到高的无症状MSSA携带率。检测到的MSSA菌株表现出高度的遗传变异,其中许多菌株携带毒力和抗生素抗性基因。虽然学生对患者的风险没有增加,但对无症状车厢人员的卫生要求有所提高。MRSA的总体流行率很低,传播的风险很小。
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引用次数: 0
Diagnostic and therapeutic strategies in combating implanted medical device-associated bacterial biofilm infections. 应对植入式医疗器械相关细菌生物膜感染的诊断和治疗策略。
IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-01-27 DOI: 10.1007/s12223-025-01242-y
Ayush Amod, Ananya Anurag Anand, Amaresh Kumar Sahoo, Sintu Kumar Samanta

Bacterial biofilms exhibit remarkable resistance against conventional antibiotics and are capable of evading the humoral immune response. They account for nearly 80% of chronic infections in humans. Development of bacterial biofilms on medical implants results in their malfunctioning and subsequently leads to high mortality rates worldwide. Therefore, early and precise diagnosis of bacterial biofilms on implanted medical devices is essential to prevent their failure and associated complications. Culture-based methods are time consuming, more prone to contamination and often exhibit low sensitivity. Different molecular, imaging, and physical methods can aid in more accurate and faster detection of implant-associated bacterial biofilms. Biofilm growth on implant surface can be prevented either through modification of the implant material or by application of different antibacterial coatings on implant surface. Experimental studies have shown that pre-existing biofilms from medical implants can be removed by breaking down biofilm matrix, utilizing physical methods, nanomaterials and antimicrobial peptides. The current review delves into mechanism of biofilm formation on implanted medical devices and the subsequent host immune response. Much emphasis has been laid on different ongoing diagnostic and therapeutic strategies to achieve improved patient outcomes and reduced socio-economic burden.

{"title":"Diagnostic and therapeutic strategies in combating implanted medical device-associated bacterial biofilm infections.","authors":"Ayush Amod, Ananya Anurag Anand, Amaresh Kumar Sahoo, Sintu Kumar Samanta","doi":"10.1007/s12223-025-01242-y","DOIUrl":"https://doi.org/10.1007/s12223-025-01242-y","url":null,"abstract":"<p><p>Bacterial biofilms exhibit remarkable resistance against conventional antibiotics and are capable of evading the humoral immune response. They account for nearly 80% of chronic infections in humans. Development of bacterial biofilms on medical implants results in their malfunctioning and subsequently leads to high mortality rates worldwide. Therefore, early and precise diagnosis of bacterial biofilms on implanted medical devices is essential to prevent their failure and associated complications. Culture-based methods are time consuming, more prone to contamination and often exhibit low sensitivity. Different molecular, imaging, and physical methods can aid in more accurate and faster detection of implant-associated bacterial biofilms. Biofilm growth on implant surface can be prevented either through modification of the implant material or by application of different antibacterial coatings on implant surface. Experimental studies have shown that pre-existing biofilms from medical implants can be removed by breaking down biofilm matrix, utilizing physical methods, nanomaterials and antimicrobial peptides. The current review delves into mechanism of biofilm formation on implanted medical devices and the subsequent host immune response. Much emphasis has been laid on different ongoing diagnostic and therapeutic strategies to achieve improved patient outcomes and reduced socio-economic burden.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Interpreting the role of epigallocatechin-3-gallate in Epstein-Barr virus infection-mediated neuronal diseases.
IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-01-23 DOI: 10.1007/s12223-025-01240-0
Annu Rani, Vaishali Saini, Nfor Gael Njini, Amit Kumar Dixit, Ajay Kumar Meena, Hem Chandra Jha

The increasing prevalence of neurodegenerative diseases is a formidable task due to their multifactorial causation and treatments limited to disease maintenance and progression. Epstein-Barr virus (EBV) is reported to be involved with neuropathologies; previous studies from our group suggested the effective binding of epigallocatechin-3-gallate (EGCG) with EBV nuclear antigen 1 (EBNA1) and glycoprotein H (gH). Therefore, in the current study, we evaluated the anti-EBV effect of ECGG on the neuronal cells. EBV-GFP exhibited a decline after EGCG treatment. We have observed a decrease in specific latent and lytic cycle genes. EBNA1 unravelled attenuation at day 1 (D1), whereas EBNA3B, EBNA3C, BMRF1, BZLF1, and gp350 showed major downregulation in D3 compared to EBV infection. Notably, EBNA-LP has shown mitigation in both the considered time points. Inflammatory and chemokine moieties like IL-6, CCR1, CCR3, and CCR5 declined upon EGCG treatment, while IL-10 exhibited elevation. Transcription factor STAT3 and NF-kB were decreased, especially in the pre-EGCG treated samples. Subsequently, restoration in the mitochondrial membrane potential was observed after EGCG treatment. We observed an increase in the mitochondrial fission genes like DRP1 and MiD49, and not many regulations were observed in the mitochondrial fusion genes except MFN2. Furthermore, the CytC, CytC oxidase, MAVS, ANT, and SDH exhibited elevation upon EGCG treatment, while ATPsyn and ABAD showed downregulation. Dysfunction of mitochondria is further related to apoptosis of neurons. Herein, we were keen to examine the level of amyloid-precursor protein (APP), and it has also indicated declined after EGCG treatment. Altogether, the current study demonstrated the anti-EBV effect of EGCG by subsiding the EBV-mediated inflammation and amendments in the neuropathological markers.

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引用次数: 0
Boosting the catalytic efficiency of UGT51 for efficient production of rare ginsenoside Rh2. 提高UGT51催化高效生产稀有人参皂苷Rh2的效率。
IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-01-22 DOI: 10.1007/s12223-025-01241-z
Mohamed Yassin Ali, Mohnad Abdalla, Ahmed F Roumia, Mohamed A Tammam, Mohamed Fawzy Ramadan, Mohmmed Abdelssalam Hassan Edrees, Atul Kabra, Daochen Zhu

Ginsenoside Rh2(S) is well-known for its therapeutic potential against diverse conditions, including some cancers, inflammation, and diabetes. The enzymatic activity of uridine diphosphate glycosyltransferase 51 (UGT51) from Saccharomyces cerevisiae plays a pivotal role in the glycosylation process between UDP-glucose (donor) and protopanaxadiol (acceptor), to form ginsenoside Rh2. However, the catalytic efficiency of the UGT51 has remained a challenging task. To this end, we employed site-directed mutagenesis on UGT51 to improve its catalytic efficiency for enhanced production of ginsenoside Rh2. The mutated structure, featuring four key mutations (E805A, S998A, R1031A, and L1032A), exhibited heightened stability, binding affinity, and active site accessibility for protopanaxadiol (PPD) compared to the wild type. Under in vitro conditions, three mutants (E805A, R1031A, and L1032A) demonstrated 10%, 58%, and 65% higher enzymatic activities compared to the wild strain. Notably, the double mutant R1031A/L1032A exhibited an 85% increase in activity. Employing a fed-batch technology with PPD as the substrate yielded a Rh2 production of 4.663 g/L. The molecular dynamics (MD) simulations were employed to investigate the movements and dynamic dynamics of UGT51 mutations and PPD complexes. The root mean square deviation (RMSD) analysis revealed substantial alterations in structural conformation, particularly in the R1031A/L1032A mutations, correlating with boosted catalytic efficiency. Furthermore, the root mean square fluctuation (RMSF) simulation study aligned with both the RMSD and the solvent-accessible surface area (SASA) analyses. The computationally guided site-directed mutagenesis approach holds promise for extending its application to the development of commercially significant enzymes.

人参皂苷Rh2(S)因其治疗多种疾病的潜力而闻名,包括一些癌症、炎症和糖尿病。酿酒酵母尿苷二磷酸糖基转移酶51 (UGT51)的酶活性在葡萄糖(供体)和原人参二醇(受体)形成人参皂苷Rh2的糖基化过程中起关键作用。然而,UGT51的催化效率仍然是一个具有挑战性的任务。为此,我们对UGT51进行定点诱变,以提高其催化效率,从而提高人参皂苷Rh2的产量。该突变结构具有4个关键突变(E805A、S998A、R1031A和L1032A),与野生型相比,具有更高的稳定性、结合亲和力和活性位点可及性。在体外条件下,三个突变体(E805A, R1031A和L1032A)的酶活性比野生菌株高10%,58%和65%。值得注意的是,双突变体R1031A/L1032A的活性增加了85%。采用以PPD为底物的补料批工艺,Rh2产量为4.663 g/L。采用分子动力学(MD)模拟研究了UGT51突变和PPD复合物的运动和动力学。均方根偏差(RMSD)分析显示,结构构象发生了实质性变化,特别是在R1031A/L1032A突变中,这与催化效率的提高有关。此外,均方根波动(RMSF)模拟研究与RMSD和溶剂可及表面积(SASA)分析一致。计算指导的定点诱变方法有望将其应用扩展到商业上重要的酶的开发。
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引用次数: 0
Clinical characteristics and risk factors for the acquisition of an enteric infection by Aeromonas spp. in patients with digestive or nephrological diseases. 消化道或肾脏疾病患者气单胞菌肠道感染的临床特征及危险因素
IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-01-03 DOI: 10.1007/s12223-024-01239-z
Isabel Lara-Plaza, Emilio Rodrigo-Calabia, Antonio Cuadrado-Lavín, Carlos Ruiz de Alegría-Puig

The role of aeromonads as contributors to gastrointestinal pathology remains controversial. The aim of this study was to analyse the clinical characteristics and risk factors for the acquisition of an enteric infection by Aeromonas spp. in patients with digestive or nephrological diseases. The method user for the study comprised a retrospective review of the clinical history of all patients in whom Aeromonas spp. was isolated in faeces. The study period included in samples arriving at the microbiology service of the Marqués de Valdecilla University Hospital, from 2016 to 2022. The results showed that there was an increase in the more virulent Aeromonas species in the patients studied. The most common chronic diseases were cancer, inflammatory bowel disease and alcoholic cirrhosis, as well as biliary involvement in acute cases. In conclusión, Aeromonas is a genus to consider in patients with diarrhoea and hepatonephrological involvement.

气单胞菌在胃肠道病理中的作用仍有争议。本研究的目的是分析消化道或肾脏疾病患者气单胞菌肠道感染的临床特征和危险因素。该研究的方法使用者包括对所有从粪便中分离出气单胞菌的患者的临床病史进行回顾性审查。研究期间包括从2016年到2022年到达马奎斯瓦德西拉大学医院微生物服务部的样本。结果表明,在研究的患者中,毒性更强的气单胞菌种类有所增加。最常见的慢性疾病是癌症、炎症性肠病和酒精性肝硬化,急性病例中胆道受累。在conclusión,气单胞菌属是考虑腹泻和肝肾病变患者。
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引用次数: 0
Mixed-species Pseudomonas biofilms: a novel and sustainable strategy for malachite green dye decolorization and detoxification. 混合种假单胞菌生物膜:孔雀石绿染料脱色和解毒的一种新颖和可持续的策略。
IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-01-02 DOI: 10.1007/s12223-024-01238-0
Nabanita Ghosh, Arup Ratan Biswas, Arindam Chakraborty, Arnab Ganguli

This study investigated the application of mixed biofilms formed by two Pseudomonas strains (NAA22 and NAA23) for bio-decolorization of malachite green (MG) dye. The isolated strains displayed biofilm formation and MG decolorization capabilities. Mixed biofilms exhibited significantly greater biofilm formation and MG decolorization (94.3%) compared to individual strains, suggesting synergistic interactions. This decolorization efficiency surpassed previously reported values for single strain decolorization. The mixed biofilms tolerated a broad range of temperatures (20-40 °C) and pH (5-9), with optimal decolorization at neutral or slightly acidic conditions (pH7.0). Enzyme analysis revealed laccase, NADH-DCIP reductase, and azoreductase as key contributors to MG decolorization, with significantly higher activity in mixed biofilms. Importantly, the bio-decolorization process transformed MG into non-phytotoxic compounds, demonstrated by seed germination and growth assays. These findings propose a promising and environmentally safe approach for MG bioremediation using mixed Pseudomonas biofilms.

研究了由NAA22和NAA23假单胞菌组成的混合生物膜对孔雀石绿染料的生物脱色效果。分离菌株表现出生物膜形成和MG脱色能力。与单个菌株相比,混合生物膜的生物膜形成和MG脱色率显著提高(94.3%),表明两者之间存在协同作用。这种脱色效率超过了以前报道的单菌株脱色值。混合生物膜可耐受广泛的温度(20-40℃)和pH(5-9),在中性或微酸性条件下(pH7.0)脱色效果最佳。酶分析表明,漆酶、NADH-DCIP还原酶和偶氮还原酶是MG脱色的关键酶,在混合生物膜中活性显著提高。重要的是,通过种子萌发和生长试验,生物脱色过程将MG转化为非植物毒性化合物。这些发现为使用混合假单胞菌生物膜进行MG生物修复提供了一种有前景且环境安全的方法。
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引用次数: 0
In vitro detoxification of aflatoxin B1 by Lactiplantibacillus plantarum isolated from the north of Iran: A pioneering insights into the origin of fermented beverages. 从伊朗北部分离的植物乳杆菌体外解毒黄曲霉毒素B1:对发酵饮料起源的开创性见解。
IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-12-30 DOI: 10.1007/s12223-024-01234-4
Abdoljalil Eiri, Hami Kaboosi, Farhad Niknejad, Abdollah Ardebili, Hamid Reza Joshaghani

The contamination of food and animal feeds with mycotoxions, particularly aflatoxin B1 (AFB1), poses significant risks to human health and causes economic losses. This study investigated bacteria from various fermented milk products to assess their ability to detoxify AFB1. A variety of household fermented kefir milk, kefir-like beverages, and kefir grains were collected from rural areas and subjected to microbiological analysis. Gram-positive bacterial isolates were further identified based on the 16S rRNA gene homology analysis. Seven bacterial isolates that were initially identified as lactic acid bacteria were selected for their potential to detoxify AFB1. Effects of environmental factors, including temperature, time, pH, and cell concentration, as well as bacterial components such as inoculum, fermentation supernatant, and cells, were evaluated on AFB1 detoxification. The most frequent isolates belonged to the new genus Lentilactobacillus and Lactiplantibacillus, of which three strains were identified as L. kefiri, L. diolivorans, and L. plantarum. The selected L. plantarum isolate demonstrated optimal AFB1 detoxification at pH 4, a 4-h exposure time, and a cell concentration of 1.0 × 1016 CFU/mL. Significant differences were observed in toxin removal between fermentation supernatant and cells, while temperature showed no significant effect on toxin detoxification. This study demonstrated the high ability of L. plantarum for AFB1 detoxification, suggesting potential applications for food and feed safety enhancement. Further research is warranted to optimize its effectiveness and explore broader applications.

霉菌毒素,特别是黄曲霉毒素B1 (AFB1)污染食品和动物饲料,对人类健康构成重大风险,并造成经济损失。本研究调查了各种发酵乳制品中的细菌,以评估它们解毒AFB1的能力。从农村地区收集了各种家用发酵开菲尔牛奶、类似开菲尔的饮料和开菲尔谷物,并进行了微生物分析。根据16S rRNA基因同源性分析进一步鉴定革兰氏阳性菌分离株。7种最初被鉴定为乳酸菌的细菌分离株被选中,因为它们具有解毒AFB1的潜力。评估环境因素(包括温度、时间、pH和细胞浓度)以及细菌成分(如接种量、发酵上清液和细胞)对AFB1解毒的影响。最常见的分离菌株为Lentilactobacillus和Lactiplantibacillus新属,其中鉴定出3株为L. kefiri、L. diolivorans和L. plantarum。所选植物乳杆菌分离物在pH为4、暴露时间为4 h、细胞浓度为1.0 × 1016 CFU/mL的条件下对AFB1脱毒效果最佳。发酵上清液和细胞对毒素的去除率存在显著差异,而温度对毒素的去除率无显著影响。本研究证明了植物乳杆菌对AFB1的高解毒能力,为提高食品和饲料的安全性提供了潜在的应用前景。有必要进一步研究以优化其有效性并探索更广泛的应用。
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Folia microbiologica
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