Foods最新文献

The aim of this study was to compare the effects of glycation and ultra-high pressure (UHP) treatment on the structure and IgG immunobinding activity of Salangidae icefish PV. The Circular Dichroism (CD) and Fluorescence Spectroscopy (FS) findings indicated that the glycation significantly affected both the secondary and tertiary structures of PV. However, the impact of UHP processing on the structure of PV was found to be less significant compared to the glycation. Western Blot analysis also revealed that the glycation markedly reduced the antigen specificity of PV. Conversely, UHP treatments at 300 MPa and 400 MPa slightly decreased the antigen specificity, whereas lower or excessively high pressures did not have a substantial impact. This research contributes valuable insights into strategies for reducing the allergenic potential of Salangid icefish.

Food safety continues to be a global concern threatening human life, especially in low-income countries where frequent electricity outages pose higher risks to food safety, increasing the risks of foodborne illnesses due to temperature fluctuations. This study aimed to assess the consumer's knowledge of food safety, beliefs, and household practices during electricity cut-off. A cross-sectional study among consumers in Lebanon was conducted using an online survey (n = 571). Results revealed that food handlers in Lebanon had unsatisfactory food safety knowledge levels along with poor food safety beliefs and practices. The findings also showed that good knowledge scores were significantly associated with age, governorate, educational level, a self-reported food safety knowledge score, and the frequency of checking the temperature of fridges/freezers (p < 0.001). This study exposed inadequate food safety knowledge and deficient food safety-related beliefs and practices among participants in Lebanon, particularly during periods of electricity outages. These gaps highlight the need for educational interventions and structured efforts to enhance participants' understanding of safe food handling and storage practices under challenging conditions to reduce the risk of foodborne illnesses and improve the public health outcomes in Lebanon.

Peanut protein is a byproduct of peanut oil extraction with limited applications within the food sector due to its low solubility and emulsifying properties. This study investigated the influences and mechanisms of high-intensity ultrasound (HIU, 200~600 W) and pH-shifting (pH 12), either individually or jointly, on the structure, solubility, and emulsifying properties of PP. Results indicated that the solubility of PP significantly increased after the combined treatment, particularly when the HIU power was 300 W (p < 0.05). Accordingly, emulsions prepared from it exhibited highest storage stability. Structural analysis indicated that the increased PP solubility (9.95% to 54.37%, p < 0.05) is mainly attributed to the structural changes that occur during protein unfolding, resulting in the uncovering of hydrophobic groups (7181.43 to 14,083.00, p < 0.05) and the reduction of α-helices (24.43% to 18.17%, p < 0.05). Moreover, confocal laser scanning microscopy of the emulsions revealed that the combination-treated PP resulted in smaller protein particle sizes (50.09 μm to 15.68 μm, p < 0.05), tighter adsorption on the oil-water interface, and a denser and more stable interfacial film compared to the native and the individual treatment, thereby enhancing the stability of the system. A rheological analysis confirmed that the combined treatment improved the interfacial properties of the protein, which was advantageous for emulsion stability. In conclusion, HIU combined with pH₁₂-shifting can appreciably improve the solubility and emulsifying properties of PP to broaden its application prospects.

The binding interactions between okadaic acid (OA) aptamers and OA molecules are crucial for developing effective detection methods. This study aims to identify the recognition site and establish a reliable detection protocol through computational simulations and experimental validations. After determining the target sequence (OA-2), molecular docking simulations using Sybyl-X and H-dock were conducted to predict the binding affinity and interaction sites of OA aptamers with their targets. These predictions were subsequently validated through experiments based on the Förster resonance energy transfer (FRET) principle. The combined approach not only confirmed the computational predictions, identifying the "major region" as the recognition basis of OA-2, but also provided deeper insights into the binding mechanisms. Subsequently, a classical AuNPs-aptamer colorimetric detection method was established based on the OA-2 sequence and applied to the detection of real shellfish samples, achieving a limit of quantification (LOQ) of 5.0 μg kg^-1. The recoveries of OA in spiked samples ranged from 79.0% to 122.9%, with a relative standard deviation (RSD) of less than 14.7%. The results of this study contribute to the development of robust detection methods for OA aptamer-target interactions, enhancing the potential for practical applications in toxin detection and monitoring.

This study focuses on the extraction of betalain compounds from Opuntia stricta as a natural alternative to synthetic colorants and sustainable environmentally friendly technology solutions. Non-conventional extraction technologies including microwave (MW) and ultrasound (US) were used alone or in combination. The extraction process was conducted for both undried Opuntia stricta (OS) and dried Opuntia stricta (DOS) plant material at two distinct drying temperatures, 40 °C and 60 °C, to assess the stability of betalain molecules. The colorant's potential was evaluated by determining the betalain content, total phenolic content, and antioxidant activity. The MW (2 min) and MW (2 min) + US (10 min) extraction processes yielded the greatest betalain content in OS fresh weight (FW), with 48.54 ± 0.29 mg/100 g FW and 51.01 ± 0.16 mg/100 g FW, respectively. Furthermore, the results showed a considerable drop in betalain content when the plant material was dried at 40 °C and 60 °C, with reduction rates of 53.75% and 24.82%, respectively, compared to the betalain content before the drying process. The LC-DAD-ESI-MS analysis supported this result, revealing the presence of 17-decarboxy betanin, 17-decarboxy neobetanin, and Cyclo-dopa5-O-βglucoside in DOS at 40 °C. This study highlights the potential future in the sustainable green extraction of betalain compounds with less heat degradation to offer a stable natural colorant.

This study aimed to evaluate the feasibility of producing and characterizing Cistus creticus L. powders obtained through spray drying and freeze drying using maltodextrin and inulin as carriers. Quantitative and qualitative analysis of polyphenols by high-performance liquid chromatography with diode-array detection (HPLC-DAD) and high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) identified key bioactive compounds, including punicalagin isomers and their galloyl esters, as well as flavonoids (myricetin-3-galactoside, myricetin-3-rhamnoside, quercetin-3-galactoside, and tiliroside). Phenolics in powders produced by both drying techniques ranged from 73.2 mg to 78.5 mg per g of dry matter. Inulin proved to be as effective as maltodextrin in spray drying, offering a promising alternative for plant-based powder formulation. Antioxidant capacity measured by Trolox equivalent antioxidant capacity assay with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (TEAC ABTS) and ferric reducing antioxidant power (FRAP) assay indicated that spray-dried powders with inulin exhibited antioxidant properties comparable to those with maltodextrin. The results demonstrated that Cistus creticus L. powders obtained with inulin can serve as valuable sources of bioactive compounds with potential health benefits similar to those obtained with maltodextrin. Moreover, from a technological perspective, inulin proved to be an equally efficient carrier in terms of production-process parameters such as moisture content and water activity, making it a viable alternative to maltodextrin in plant-based powder formulations.

Due to their markedly distinct protein compositions and structures, goat milk and cow milk display substantially different characteristics. In this study, the quality and composition of goat milk and cow milk were studied after being refrigerated at 4 °C for 7 days, with a particular focus on protein oxidation and aggregation states. The results revealed that alongside increases in acidity, microbial colony count, and hydrolysis, there was a significant change in the protein aggregation state beginning on the second day. This change was characterized by increased turbidity, an elevated centrifugal sedimentation rate, and a right-shifted particle size distribution. After seven days of refrigeration, the centrifugal sedimentation rate of goat milk increased from 0.53% to 0.97%, whereas that of cow milk rose from 0.41% to 0.58%. The degree of aggregation was significantly greater in goat milk compared to cow milk. Additionally, both protein and lipids exhibited substantial oxidation, with the degree of oxidation more pronounced in goat milk than in cow milk. The malondialdehyde (MDA) content increased from 0.047 μg/mL to 0.241 μg/mL in goat milk and from 0.058 μg/mL to 0.178 μg/mL in cow milk. The results suggest that goat milk was more prone to oxidation, which further reduced its stability. Therefore, in the storage and transportation of dairy products before processing, it is essential not only to monitor sanitary conditions but also to effectively control protein oxidation to enhance the quality of milk processing.

Lentils represent a promising alternative for beer production, potentially offering unique benefits and challenges. This study investigates the physicochemical properties of brewer's wort derived from both barley and lentil grains. Specifically, it compares worts produced from raw and malted lentils, with and without the addition of amylase and protease enzymes. Key parameters such as filtration and saccharification times, pH, extract content, color, turbidity, polyphenol content, free amino nitrogen (FAN), nitrogen content, and metal ion and sugar composition were meticulously measured. Results indicate that both raw and malted lentils can be utilized to produce brewer's wort, with the malting process enhancing extract levels. Notably, the addition of amylolytic enzymes resulted in the highest extract levels for both lentil types. Lentil-based worts exhibited significantly higher FAN levels and lower turbidity compared to barley malt worts. Despite barley malt's established advantages in saccharification efficiency, filtration, and extract yield, lentils offer distinct benefits such as elevated FAN levels and unique color profiles. Enzyme treatments play a crucial role in optimizing lentil-based wort production, highlighting the potential for lentils in brewing applications.

Bitter melon seed oil (BMSO), as a by-product of bitter gourd fruit processing, is rich in active ingredients and has unique medicinal potential. However, its solubility and dispersibility in water are poor when used directly. Therefore, this study aims to develop an eco-friendly submicron emulsion containing BMSO for intravenous injection and evaluate its safety. The BMSO submicron emulsion (BMSOSE) was prepared by high-pressure homogenization. The size, polydispersity index (PDI), ζ-potential, Turbiscan stability index (TSI), apparent viscosity, and morphology were characterized; in addition, an in vitro hemolysis test and acute toxicity test in mice were investigated in detail to evaluate the emulsion. The results demonstrated that the formulation and technological parameters of the BMSOSE were as follows: BMSO, 8% (w/w); egg yolk lecithin, 1.2% (w/w); F-68, 0.2% (w/w); pH, 5.0; homogenization pressure, 600 Pa; and number of homogenization cycle, 9. The obtained BMSOSE droplets exhibited a spherical shape with uniform size distribution with an average diameter of 221.3 nm, a PDI of 0.2, and a ζ-potential of -36 mV. There was no significant change in the fatty acid composition of BMSO and the BMSOSE. The safety tests demonstrated that the BMSOSE had no signs of hemolysis and had no toxicity to mice with LD50 > 64 mL/kg. This study provides a foundation for further development of BMSO and its preparations.

By 2050, the world's population will rise to 9 billion, which implies that it is necessary to double protein production. We should consider more sustainable, alternative forms of protein. A solution to this is the use of insects, which offer high levels of protein and require less water than poultry, pork, and beef production. The objective of this study was to evaluate 13 countries' consumer perceptions regarding the willingness to eat specific types of insects as powdered ingredients in five food types. An online survey was conducted using Check All That Apply (CATA) to assess consumer perceptions across 13 countries. Approximately 630 consumers in each country were surveyed (total n > 8100). The CATA data were analyzed using Cochran's Q test, which showed highly significant differences among countries. The willingness to eat insects varied by country, food type, and insect species. The results of this study can be used to understand consumers' perceptions of insects and offer an indicator that can be used when developing insect-containing foods in the future.