The chemical composition of honey, and therefore its quality and properties, is influenced by many factors, including its botanical origin and the harvesting conditions-the location of the apiary, access to melliferous plants, the proximity of industrial infrastructure and communication routes, etc. This quality may be reduced by undesirable, toxic compounds that penetrate honey from a contaminated environment, such as heavy metals and residues from other environmental pollutants. Therefore, the aim of our research was to assess the quality of honeys from urbanized areas-in particular, to assess contamination with heavy metals and persistent organic pollutants (PAHs). In total, 35 samples from six different apiaries located in urbanized areas were examined. The content of heavy metals (Cd, Pb, Zn) was determined by atomic absorption spectrophotometry (AAS), and the content of total PAHs as the sum of the concentrations of the compounds benzo(a)anthracene, chrysene, benzo(b)fluoranthene and benzo(a)pyrene was determined by high-performance liquid chromatography with fluorescence detection (HPLC-FLD). The average zinc content ranged from about 2 to 4.5 mg/kg, the average lead content ranged from 3.5 µg/kg to 388 µg/kg and the average cadmium content ranged from 0.5 to 14 µg/kg. It was found that all honeys contained certain amounts of harmful metals, and only lead exceeded the permissible limits. None of the samples tested contained sum content of PAHs exceeding 10 µg/kg of honey. Contrary to our expectations, the results obtained indicate that honeys from urbanized areas do not contain these harmful substances. In general, the presence of harmful metals does not, however, reduce honey's quality or its health value.
{"title":"Content of Selected Harmful Metals (Zn, Pb, Cd) and Polycyclic Aromatic Hydrocarbons (PAHs) in Honeys from Apiaries Located in Urbanized Areas.","authors":"Aleksandra Wilczyńska, Natalia Żak, Ewa Stasiuk","doi":"10.3390/foods13213451","DOIUrl":"10.3390/foods13213451","url":null,"abstract":"<p><p>The chemical composition of honey, and therefore its quality and properties, is influenced by many factors, including its botanical origin and the harvesting conditions-the location of the apiary, access to melliferous plants, the proximity of industrial infrastructure and communication routes, etc. This quality may be reduced by undesirable, toxic compounds that penetrate honey from a contaminated environment, such as heavy metals and residues from other environmental pollutants. Therefore, the aim of our research was to assess the quality of honeys from urbanized areas-in particular, to assess contamination with heavy metals and persistent organic pollutants (PAHs). In total, 35 samples from six different apiaries located in urbanized areas were examined. The content of heavy metals (Cd, Pb, Zn) was determined by atomic absorption spectrophotometry (AAS), and the content of total PAHs as the sum of the concentrations of the compounds benzo(a)anthracene, chrysene, benzo(b)fluoranthene and benzo(a)pyrene was determined by high-performance liquid chromatography with fluorescence detection (HPLC-FLD). The average zinc content ranged from about 2 to 4.5 mg/kg, the average lead content ranged from 3.5 µg/kg to 388 µg/kg and the average cadmium content ranged from 0.5 to 14 µg/kg. It was found that all honeys contained certain amounts of harmful metals, and only lead exceeded the permissible limits. None of the samples tested contained sum content of PAHs exceeding 10 µg/kg of honey. Contrary to our expectations, the results obtained indicate that honeys from urbanized areas do not contain these harmful substances. In general, the presence of harmful metals does not, however, reduce honey's quality or its health value.</p>","PeriodicalId":12386,"journal":{"name":"Foods","volume":"13 21","pages":""},"PeriodicalIF":4.7,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11545775/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142616679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yamin Wu, Xinghua Wang, Lijiao Chen, Qiang Li, Junjie He, Xiujuan Deng, Jiayi Xu, Raoqiong Che, Jianyun Zhou, Wenxia Yuan, Tianyu Wu, Juan Tian, Yaping Chen, Baijuan Wang
To explore the effects of different withering methods on the quality of Congou black tea, this study focused on five different withering methods: natural withering, warm-air withering, sun-natural combined withering, sun withering, and shaking withering. Gas chromatography‒mass spectrometry (GC‒MS), high-performance liquid chromatography (HPLC), and ion-exchange chromatography techniques were used to analyze the nonvolatile and volatile components and composition of the tea. The results revealed significant differences (p < 0.05) in the contents of nonvolatile constituents including caffeine, polyphenols, soluble sugars, free amino acids and their components, theaflavins, thearubigins, and catechins among the five different withering methods, with varying degrees of correlation between these components. A total of 227 aroma compounds were detected, and significant differences in the contents of alcohols, aldehydes, and ketones were observed. A relative odor activity value (ROVA) analysis of the aroma compounds revealed that 19 compounds had an ROVA > 1. Among them, benzylaldehyde, trans-2-decenal, decanal, benzaldehyde, nonanal, hexanal, trans-linalool, and geraniol from the shaking withering method had significantly higher ROVA values than those from the other withering methods, which may be the reason for the prominent floral and fruity aroma of shaking withering. This study revealed the impact of different withering methods on the quality of Congou black tea, providing a scientific basis for the development of Congou black tea with different flavors and the improvement of Congou black tea processing techniques.
{"title":"Effects of Five Different Withering Methods on the Composition and Quality of Congou Black Tea.","authors":"Yamin Wu, Xinghua Wang, Lijiao Chen, Qiang Li, Junjie He, Xiujuan Deng, Jiayi Xu, Raoqiong Che, Jianyun Zhou, Wenxia Yuan, Tianyu Wu, Juan Tian, Yaping Chen, Baijuan Wang","doi":"10.3390/foods13213456","DOIUrl":"10.3390/foods13213456","url":null,"abstract":"<p><p>To explore the effects of different withering methods on the quality of Congou black tea, this study focused on five different withering methods: natural withering, warm-air withering, sun-natural combined withering, sun withering, and shaking withering. Gas chromatography‒mass spectrometry (GC‒MS), high-performance liquid chromatography (HPLC), and ion-exchange chromatography techniques were used to analyze the nonvolatile and volatile components and composition of the tea. The results revealed significant differences (<i>p</i> < 0.05) in the contents of nonvolatile constituents including caffeine, polyphenols, soluble sugars, free amino acids and their components, theaflavins, thearubigins, and catechins among the five different withering methods, with varying degrees of correlation between these components. A total of 227 aroma compounds were detected, and significant differences in the contents of alcohols, aldehydes, and ketones were observed. A relative odor activity value (ROVA) analysis of the aroma compounds revealed that 19 compounds had an ROVA > 1. Among them, benzylaldehyde, trans-2-decenal, decanal, benzaldehyde, nonanal, hexanal, trans-linalool, and geraniol from the shaking withering method had significantly higher ROVA values than those from the other withering methods, which may be the reason for the prominent floral and fruity aroma of shaking withering. This study revealed the impact of different withering methods on the quality of Congou black tea, providing a scientific basis for the development of Congou black tea with different flavors and the improvement of Congou black tea processing techniques.</p>","PeriodicalId":12386,"journal":{"name":"Foods","volume":"13 21","pages":""},"PeriodicalIF":4.7,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11545746/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dajiang Wang, Guangyi Wang, Xiang Lu, Zhao Liu, Simiao Sun, Hanxin Guo, Wen Tian, Zichen Li, Lin Wang, Lianwen Li, Yuan Gao, Kun Wang
Polyphenols are important functional components that have anti-cancer and anti-inflammatory effects. Apple fruit is rich in polyphenols and is one of the dietary sources of polyphenols. The polyphenol components and contents of the peel and pulp of 74 Malus sieversii (Led.) Roem. and 26 Chinese Malus germplasm resources were determined using ultra-high-phase chromatography (UPLC) and liquid chromatography-mass spectrometry (LC-MS). The results showed that 34 components were detected in the peel and 30 in the flesh, and that the polyphenol components and contents of the different germplasm resources were significantly different; the polyphenol content of Malus sieversii (Led.) Roem. was significantly higher than that of the other local varieties, and the polyphenol content in the peel was also higher than that in the flesh. Rutin, quercetin 3-O-arabopyranoside, kaempferol 3-O-rutinoside, and peonidin 3-O-galactoside were detected only in the peel. The total polyphenol content in the peel ranged from 949.76 to 5840.06 mg/kg, and the polyphenol content in the pulp ranged from 367.31 to 5123.10 mg/kg. The cluster analysis of polyphenol components and contents in peel and pulp showed that 100 Malus germplasm resources could be grouped into four categories. Principal component analysis of 34 kinds and 30 kinds of polyphenols in peel and pulp of 100 resources was performed. If the eigenvalue is greater than 1, eight and seven principal components are extracted, respectively. Five Malus resources with high polyphenol content in the peel and pulp were selected: 'XY-77' (peel: 5840.06 mg/kg, pulp: 5123.10 mg/kg; 'LF-09' (peel: 4692.63 mg/kg, pulp: 3729.79 mg/kg); '2012-5' (peel: 4377.61 mg/kg, pulp: 3847.54 mg/kg); '29028' (peel: 5088.05 mg/kg, pulp: 3994.61 mg/kg); and '11-01' (peel: 5154.45 mg/kg, pulp: 3616.15 mg/kg). These results provide us with information regarding the polyphenol composition and content of the wild apple resources and local cultivars. The high polyphenol content resources obtained by screening can be used as raw materials for the extraction of polyphenol components and functional fruit juice processing and can also be used as parents for functional fruit creation and variety breeding.
{"title":"Identification and Mining of Functional Components of Polyphenols in Fruits of <i>Malus</i> Germplasm Resources Based on Multivariate Analysis.","authors":"Dajiang Wang, Guangyi Wang, Xiang Lu, Zhao Liu, Simiao Sun, Hanxin Guo, Wen Tian, Zichen Li, Lin Wang, Lianwen Li, Yuan Gao, Kun Wang","doi":"10.3390/foods13213465","DOIUrl":"10.3390/foods13213465","url":null,"abstract":"<p><p>Polyphenols are important functional components that have anti-cancer and anti-inflammatory effects. Apple fruit is rich in polyphenols and is one of the dietary sources of polyphenols. The polyphenol components and contents of the peel and pulp of 74 <i>Malus sieversii</i> (Led.) Roem. and 26 Chinese <i>Malus</i> germplasm resources were determined using ultra-high-phase chromatography (UPLC) and liquid chromatography-mass spectrometry (LC-MS). The results showed that 34 components were detected in the peel and 30 in the flesh, and that the polyphenol components and contents of the different germplasm resources were significantly different; the polyphenol content of <i>Malus sieversii</i> (Led.) Roem. was significantly higher than that of the other local varieties, and the polyphenol content in the peel was also higher than that in the flesh. Rutin, quercetin 3-O-arabopyranoside, kaempferol 3-O-rutinoside, and peonidin 3-O-galactoside were detected only in the peel. The total polyphenol content in the peel ranged from 949.76 to 5840.06 mg/kg, and the polyphenol content in the pulp ranged from 367.31 to 5123.10 mg/kg. The cluster analysis of polyphenol components and contents in peel and pulp showed that 100 <i>Malus</i> germplasm resources could be grouped into four categories. Principal component analysis of 34 kinds and 30 kinds of polyphenols in peel and pulp of 100 resources was performed. If the eigenvalue is greater than 1, eight and seven principal components are extracted, respectively. Five <i>Malus</i> resources with high polyphenol content in the peel and pulp were selected: 'XY-77' (peel: 5840.06 mg/kg, pulp: 5123.10 mg/kg; 'LF-09' (peel: 4692.63 mg/kg, pulp: 3729.79 mg/kg); '2012-5' (peel: 4377.61 mg/kg, pulp: 3847.54 mg/kg); '29028' (peel: 5088.05 mg/kg, pulp: 3994.61 mg/kg); and '11-01' (peel: 5154.45 mg/kg, pulp: 3616.15 mg/kg). These results provide us with information regarding the polyphenol composition and content of the wild apple resources and local cultivars. The high polyphenol content resources obtained by screening can be used as raw materials for the extraction of polyphenol components and functional fruit juice processing and can also be used as parents for functional fruit creation and variety breeding.</p>","PeriodicalId":12386,"journal":{"name":"Foods","volume":"13 21","pages":""},"PeriodicalIF":4.7,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11545546/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Raman K Bhaskaracharya, Fatima Saeed Rashed Alnuaimi, Shaikha Rashed Juma Aldarmaki, Abeena Abdulazeez, Mutamed Ayyash
Labneh is a popular fermented dairy product, which contemporarily has diversified into a varied range of styles, formulated with the inclusion of multiple additives, and is sourced across the globe. This has driven labneh's emergence as a complex product with varying textural and rheological characteristics. The lack of scientific literature about labneh products available in the United Arab Emirates (UAE) market and their characterization has prompted this study. A detailed UAE market analysis of labneh for label, formulation, nutrition, and price variability was conducted. Surveyed labneh products were categorized as unpackaged, multinational company (MNC), small and medium enterprise (SME), and specialty products. They differed in manufacturing, such as acid ± enzyme coagulation with/without post-fermentation heat treatment, and contained various stabilizers, emulsifiers, preservatives, and processing aids. Interestingly, almost equal proportions, 64.7% and 67%, of MNC and SME labneh contained additives, respectively. All MNC labneh were post-heat-treated, in contrast to only 7% of SME labneh. Organic labneh and non-bovine milk-based labneh are not yet widely available. The second part of the study involved the physicochemical characterization of a select number of packaged labneh that were categorized in accordance with fat content as high-fat (17-18%), full-fat (7.1-8%), and lite-fat (3.5-4.5%). High-fat labneh showed a significantly higher complex viscosity, complex modulus, hardness, adhesiveness, stringiness, and fracturability, followed by lite-fat labneh compared to full-fat labneh, especially when it contained pectin. Full-fat labneh with added gums (and starch) and high-fat labneh with gums showed a significantly lower complex modulus compared to their respective control labneh. This study highlights the variety of commercial labneh products available and differences in their formulation, manufacturing, and composition, and provides specific dependencies of materials with their physicochemical characteristics.
{"title":"Labneh: A Retail Market Analysis and Selected Product Characterization.","authors":"Raman K Bhaskaracharya, Fatima Saeed Rashed Alnuaimi, Shaikha Rashed Juma Aldarmaki, Abeena Abdulazeez, Mutamed Ayyash","doi":"10.3390/foods13213461","DOIUrl":"10.3390/foods13213461","url":null,"abstract":"<p><p>Labneh is a popular fermented dairy product, which contemporarily has diversified into a varied range of styles, formulated with the inclusion of multiple additives, and is sourced across the globe. This has driven labneh's emergence as a complex product with varying textural and rheological characteristics. The lack of scientific literature about labneh products available in the United Arab Emirates (UAE) market and their characterization has prompted this study. A detailed UAE market analysis of labneh for label, formulation, nutrition, and price variability was conducted. Surveyed labneh products were categorized as unpackaged, multinational company (MNC), small and medium enterprise (SME), and specialty products. They differed in manufacturing, such as acid ± enzyme coagulation with/without post-fermentation heat treatment, and contained various stabilizers, emulsifiers, preservatives, and processing aids. Interestingly, almost equal proportions, 64.7% and 67%, of MNC and SME labneh contained additives, respectively. All MNC labneh were post-heat-treated, in contrast to only 7% of SME labneh. Organic labneh and non-bovine milk-based labneh are not yet widely available. The second part of the study involved the physicochemical characterization of a select number of packaged labneh that were categorized in accordance with fat content as high-fat (17-18%), full-fat (7.1-8%), and lite-fat (3.5-4.5%). High-fat labneh showed a significantly higher complex viscosity, complex modulus, hardness, adhesiveness, stringiness, and fracturability, followed by lite-fat labneh compared to full-fat labneh, especially when it contained pectin. Full-fat labneh with added gums (and starch) and high-fat labneh with gums showed a significantly lower complex modulus compared to their respective control labneh. This study highlights the variety of commercial labneh products available and differences in their formulation, manufacturing, and composition, and provides specific dependencies of materials with their physicochemical characteristics.</p>","PeriodicalId":12386,"journal":{"name":"Foods","volume":"13 21","pages":""},"PeriodicalIF":4.7,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11544886/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Carla S S Teixeira, Bruno Carriço-Sá, Caterina Villa, Joana Costa, Isabel Mafra, Isabel M P L V O Ferreira, Miguel A Faria, Tânia G Tavares
Entomophagy is being proposed as a sustainable and nutritious alternative protein source. Additionally, insect consumption is also associated with some health benefits mediated by bioactive compounds produced during gastrointestinal (GI) digestion. The antihypertensive property resulting from the inhibition of the somatic angiotensin-converting enzyme (sACE) by small peptides is one of the most common bioactivities related to insect consumption. This study aimed to investigate the potential sACE-inhibitory capacity of six peptides (AVQPCF, CAIAW, IIIGW, QIVW, PIVCF, and DVW), previously identified by the in silico GI digestion of Acheta domesticus proteins, validate their formation after in vitro GI digestion of A. domesticus by LC-MS/MS, and assess the bioactivity of the bioaccessible digesta. The results showed that the IC50 values of AVQPCF, PIVCF, and CAIAW on sACE were 3.69 ± 0.25, 4.63 ± 0.16, and 6.55 ± 0.52 μM, respectively. The obtained digesta demonstrated a sACE-inhibitory capacity of 77.1 ± 11.8 µg protein/mL extract (IC50). This is the first report of the sACE-inhibitory capacity attributed to whole A. domesticus subjected to GI digestion without any pre-treatment or protein concentration. This evidence highlights the potential antihypertensive effect of both the digesta and the identified peptides.
{"title":"Uncovering the Potential Somatic Angiotensin-Converting Enzyme (sACE) Inhibitory Capacity of Peptides from <i>Acheta domesticus</i>: Insights from In Vitro Gastrointestinal Digestion.","authors":"Carla S S Teixeira, Bruno Carriço-Sá, Caterina Villa, Joana Costa, Isabel Mafra, Isabel M P L V O Ferreira, Miguel A Faria, Tânia G Tavares","doi":"10.3390/foods13213462","DOIUrl":"10.3390/foods13213462","url":null,"abstract":"<p><p>Entomophagy is being proposed as a sustainable and nutritious alternative protein source. Additionally, insect consumption is also associated with some health benefits mediated by bioactive compounds produced during gastrointestinal (GI) digestion. The antihypertensive property resulting from the inhibition of the somatic angiotensin-converting enzyme (sACE) by small peptides is one of the most common bioactivities related to insect consumption. This study aimed to investigate the potential sACE-inhibitory capacity of six peptides (AVQPCF, CAIAW, IIIGW, QIVW, PIVCF, and DVW), previously identified by the in silico GI digestion of <i>Acheta domesticus</i> proteins, validate their formation after in vitro GI digestion of <i>A. domesticus</i> by LC-MS/MS, and assess the bioactivity of the bioaccessible digesta. The results showed that the IC<sub>50</sub> values of AVQPCF, PIVCF, and CAIAW on sACE were 3.69 ± 0.25, 4.63 ± 0.16, and 6.55 ± 0.52 μM, respectively. The obtained digesta demonstrated a sACE-inhibitory capacity of 77.1 ± 11.8 µg protein/mL extract (IC<sub>50</sub>). This is the first report of the sACE-inhibitory capacity attributed to whole <i>A. domesticus</i> subjected to GI digestion without any pre-treatment or protein concentration. This evidence highlights the potential antihypertensive effect of both the digesta and the identified peptides.</p>","PeriodicalId":12386,"journal":{"name":"Foods","volume":"13 21","pages":""},"PeriodicalIF":4.7,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11544891/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study investigated the toxigenic genes and antimicrobial resistance profiles of Staphylococcus aureus strains isolated from 260 raw milk samples collected from dairy farms in Türkiye. The results indicated that 60.7% of staphylococcal enterotoxin genes (sea, seb, sed, seg, sei, sej, sek, seq, sem, seo, and seu) and 21.4% of the tst and eta genes were positive, with most enterotoxin-positive samples carrying more than one gene. The sec, see, seh, sel, sen, sep, and etb genes were not identified in any samples. The prevalence of antibiotic resistance genes (mecA, blaR, blaI, blaZ, vanA, ermT, tetK, aac/aph, ant, dfrA, tcaR, IS256, and IS257) was high at 89.2%, with bla being the most frequently detected gene (75%). The mecA gene was present in 14.2% of samples, while tcaR was detected in 78.5%. Nevertheless, the mecC was not identified. Disinfectant resistance genes (qacA/B, qacC, qacJ, smr) were detected in 21.4% of the samples. The results of the disk diffusion test showed that 64.2% of strains were resistant to penicillin G and ampicillin, with additional resistance found for cefoxitin, teicoplanin, levofloxacin, norfloxacin, and other antibiotics. These findings highlight a significant public health and food safety risk associated with raw milk due to the presence of S. aureus strains with toxigenic genes and high antimicrobial resistance.
本研究调查了从土耳其奶牛场收集的 260 份生乳样本中分离出的金黄色葡萄球菌菌株的致毒基因和抗菌药耐药性概况。结果表明,60.7%的葡萄球菌肠毒素基因(sea、seb、sed、seg、sei、sej、sek、seq、sem、seo 和 seu)和 21.4% 的 tst 和 eta 基因呈阳性,大多数肠毒素阳性样本携带一种以上的基因。没有在任何样本中发现 sec、see、seh、sel、sen、sep 和 etb 基因。抗生素耐药基因(mecA、blaR、blaI、blaZ、vanA、ermT、tetK、aac/aph、ant、dfrA、tcaR、IS256 和 IS257)的流行率高达 89.2%,其中 bla 是最常检测到的基因(75%)。14.2% 的样本中存在 mecA 基因,78.5% 的样本中检测到 tcaR。不过,没有发现 mecC 基因。21.4%的样本中检测到耐消毒剂基因(qacA/B、qacC、qacJ、smr)。盘扩散试验结果显示,64.2%的菌株对青霉素 G 和氨苄西林具有耐药性,此外还发现了对头孢西丁、替考拉宁、左氧氟沙星、诺氟沙星和其他抗生素的耐药性。这些发现突出表明,由于金黄色葡萄球菌菌株具有致毒基因和较高的抗菌药耐药性,生牛奶存在着重大的公共卫生和食品安全风险。
{"title":"Investigation of Various Toxigenic Genes and Antibiotic and Disinfectant Resistance Profiles of <i>Staphylococcus aureus</i> Originating from Raw Milk.","authors":"Gulay Merve Bayrakal, Ali Aydin","doi":"10.3390/foods13213448","DOIUrl":"10.3390/foods13213448","url":null,"abstract":"<p><p>This study investigated the toxigenic genes and antimicrobial resistance profiles of <i>Staphylococcus aureus</i> strains isolated from 260 raw milk samples collected from dairy farms in Türkiye. The results indicated that 60.7% of staphylococcal enterotoxin genes (<i>sea</i>, <i>seb</i>, <i>sed</i>, <i>seg</i>, <i>sei</i>, <i>sej</i>, <i>sek</i>, <i>seq</i>, <i>sem</i>, <i>seo</i>, and <i>seu</i>) and 21.4% of the <i>tst</i> and <i>eta</i> genes were positive, with most enterotoxin-positive samples carrying more than one gene. The <i>sec</i>, <i>see</i>, <i>seh</i>, <i>sel</i>, <i>sen</i>, <i>sep</i>, and <i>etb</i> genes were not identified in any samples. The prevalence of antibiotic resistance genes (<i>mecA</i>, <i>blaR</i>, <i>blaI</i>, <i>blaZ</i>, <i>vanA</i>, <i>ermT</i>, <i>tetK</i>, <i>aac</i>/<i>aph</i>, <i>ant</i>, <i>dfrA</i>, <i>tcaR</i>, <i>IS256</i>, and <i>IS257</i>) was high at 89.2%, with <i>bla</i> being the most frequently detected gene (75%). The <i>mecA</i> gene was present in 14.2% of samples, while <i>tcaR</i> was detected in 78.5%. Nevertheless, the <i>mecC</i> was not identified. Disinfectant resistance genes (<i>qac</i>A/B, <i>qacC</i>, <i>qacJ</i>, <i>smr</i>) were detected in 21.4% of the samples. The results of the disk diffusion test showed that 64.2% of strains were resistant to penicillin G and ampicillin, with additional resistance found for cefoxitin, teicoplanin, levofloxacin, norfloxacin, and other antibiotics. These findings highlight a significant public health and food safety risk associated with raw milk due to the presence of <i>S. aureus</i> strains with toxigenic genes and high antimicrobial resistance.</p>","PeriodicalId":12386,"journal":{"name":"Foods","volume":"13 21","pages":""},"PeriodicalIF":4.7,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11544844/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Danial Fatchurrahman, Federico Marini, Mojtaba Nosrati, Andrea Peruzzi, Sergio Castellano, Maria Luisa Amodio, Giancarlo Colelli
Goji berry is acknowledged for its notable medicinal attributes and elevated free radical scavenger properties. Nevertheless, its susceptibility to mechanical injuries and biological disorders reduces the commercial diffusion of the fruit. A hyperspectral imaging system (HSI) was employed to identify common defects in the Vis-NIR range (400-1000 nm). The sensorial evaluation of visual appearance was used to obtain the reference measurement of defects. A supervised classification model employing PLS-DA was developed using raw and pre-processed spectra, followed by applying a covariance selection algorithm (CovSel). The classification model demonstrated superior performance in two classifications distinguishing between sound and defective fruit, achieving an accuracy and sensitivity of 94.9% and 96.9%, respectively. However, when extended to a more complex task of classifying fruit into four categories, the model exhibited reliable results with an accuracy and sensitivity of 74.5% and 77.9%, respectively. These results indicate that a method based on hyperspectral visible-NIR can be implemented for rapid and reliable methods of online quality inspection securing high-quality goji berries.
{"title":"The Potential Application of Visible-Near Infrared (Vis-NIR) Hyperspectral Imaging for Classifying Typical Defective Goji Berry (<i>Lycium barbarum</i> L.).","authors":"Danial Fatchurrahman, Federico Marini, Mojtaba Nosrati, Andrea Peruzzi, Sergio Castellano, Maria Luisa Amodio, Giancarlo Colelli","doi":"10.3390/foods13213469","DOIUrl":"10.3390/foods13213469","url":null,"abstract":"<p><p>Goji berry is acknowledged for its notable medicinal attributes and elevated free radical scavenger properties. Nevertheless, its susceptibility to mechanical injuries and biological disorders reduces the commercial diffusion of the fruit. A hyperspectral imaging system (HSI) was employed to identify common defects in the Vis-NIR range (400-1000 nm). The sensorial evaluation of visual appearance was used to obtain the reference measurement of defects. A supervised classification model employing PLS-DA was developed using raw and pre-processed spectra, followed by applying a covariance selection algorithm (CovSel). The classification model demonstrated superior performance in two classifications distinguishing between sound and defective fruit, achieving an accuracy and sensitivity of 94.9% and 96.9%, respectively. However, when extended to a more complex task of classifying fruit into four categories, the model exhibited reliable results with an accuracy and sensitivity of 74.5% and 77.9%, respectively. These results indicate that a method based on hyperspectral visible-NIR can be implemented for rapid and reliable methods of online quality inspection securing high-quality goji berries.</p>","PeriodicalId":12386,"journal":{"name":"Foods","volume":"13 21","pages":""},"PeriodicalIF":4.7,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11545047/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Domestic microwave ovens offer rapid cooking but face challenges such as non-uniform temperature distribution and hot spots. A novel solid-state heating system, which precisely controls microwave frequency and power, provides a promising alternative to traditional microwave ovens utilizing magnetron systems. This study compared the effects of solid-state microwave cooking on the quality of broccoli, red peppers, and carrots with those of traditional microwave and conventional cooking. The traditional microwave cooking used in this study operated at 2450 MHz, while the solid-state system functioned between 902 and 928 MHz. Weight loss was highest for conventional cooking, reaching a maximum of 34%, whereas microwave cooking resulted in a maximum of 11.65% and solid-state microwave cooking in 17.04%. The total phenolic content obtained through conventional cooking ranged between 61.58 and 116.51 mg GAE (gallic acid equivalents)/100 g dry basis, while microwave cooking resulted in a range of 88.04-110.92 mg, and solid-state microwave cooking achieved values between 76.14 and 122.91 mg. Furthermore, reductions in chlorophyll content were observed to be 68.2%, 25.6%, and 35.7% for conventional, microwave, and solid-state microwave cooking, respectively. Lycopene content after conventional cooking decreased to 224.73 mg/100 g dry basis, compared to 289.55 mg after microwave cooking and 242.94 mg after solid-state microwave cooking. β-carotene content showed a decrease of 14.5% in conventional cooking, while both microwave methods showed an increase of 14.7%. These results suggest that solid-state microwave cooking may have promising positive effects on food quality.
{"title":"Effects of 910 MHz Solid-State Microwave Cooking on the Quality Properties of Broccoli (<i>Brassica olearacea</i> L. var. <i>Italica</i> Plenck), Carrots (<i>Daucus carota</i> subsp. <i>Sativus</i>), and Red Peppers (<i>Capsicum annuum</i> L. cv. Kapya).","authors":"Gönül Çavuşoğlu Kaplan, Ebru Fıratlıgil","doi":"10.3390/foods13213459","DOIUrl":"10.3390/foods13213459","url":null,"abstract":"<p><p>Domestic microwave ovens offer rapid cooking but face challenges such as non-uniform temperature distribution and hot spots. A novel solid-state heating system, which precisely controls microwave frequency and power, provides a promising alternative to traditional microwave ovens utilizing magnetron systems. This study compared the effects of solid-state microwave cooking on the quality of broccoli, red peppers, and carrots with those of traditional microwave and conventional cooking. The traditional microwave cooking used in this study operated at 2450 MHz, while the solid-state system functioned between 902 and 928 MHz. Weight loss was highest for conventional cooking, reaching a maximum of 34%, whereas microwave cooking resulted in a maximum of 11.65% and solid-state microwave cooking in 17.04%. The total phenolic content obtained through conventional cooking ranged between 61.58 and 116.51 mg GAE (gallic acid equivalents)/100 g dry basis, while microwave cooking resulted in a range of 88.04-110.92 mg, and solid-state microwave cooking achieved values between 76.14 and 122.91 mg. Furthermore, reductions in chlorophyll content were observed to be 68.2%, 25.6%, and 35.7% for conventional, microwave, and solid-state microwave cooking, respectively. Lycopene content after conventional cooking decreased to 224.73 mg/100 g dry basis, compared to 289.55 mg after microwave cooking and 242.94 mg after solid-state microwave cooking. β-carotene content showed a decrease of 14.5% in conventional cooking, while both microwave methods showed an increase of 14.7%. These results suggest that solid-state microwave cooking may have promising positive effects on food quality.</p>","PeriodicalId":12386,"journal":{"name":"Foods","volume":"13 21","pages":""},"PeriodicalIF":4.7,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11545260/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fucose, fucose-containing oligosaccharides, and fucose-containing polysaccharides have been widely applied in the fields of food and medicine, including applications in Helicobacter pylori eradication and renal function protection. Fucose-containing carbohydrates (FCCs) derived from marine organisms such as seaweed, invertebrates, microalgae, fungi, and bacteria have garnered growing attention due to their diverse bioactivities and potential therapeutic applications. Marine-derived FCCs characterized by high fucose residue content and extensive sulfate substitution, including fucoidan, fucosylated chondroitin sulfate, and fucose-rich microbial exopolysaccharides, have demonstrated significant potential in promoting gastrointestinal health. This review describes the unique structural features of FCCs and summarizes their health benefits, including regulation of gut microbiota, modulation of microbial metabolism, anti-adhesion activities against H. pylori and gut pathogens, protection against inflammatory injuries, and anti-tumor activities. Additionally, this review discusses the structural characteristics that influence the functional properties and the limitations related to the activity research and preparation processes of FCCs, providing a balanced perspective on the application potential and challenges of FCCs with specific structures for the regulation of gastrointestinal health and diseases.
{"title":"Marine-Derived Fucose-Containing Carbohydrates: Review of Sources, Structure, and Beneficial Effects on Gastrointestinal Health.","authors":"Xinmiao Ren, Shenyuan Cai, Yiling Zhong, Luying Tang, Mengshi Xiao, Shuang Li, Changliang Zhu, Dongyu Li, Haijin Mou, Xiaodan Fu","doi":"10.3390/foods13213460","DOIUrl":"10.3390/foods13213460","url":null,"abstract":"<p><p>Fucose, fucose-containing oligosaccharides, and fucose-containing polysaccharides have been widely applied in the fields of food and medicine, including applications in <i>Helicobacter pylori</i> eradication and renal function protection. Fucose-containing carbohydrates (FCCs) derived from marine organisms such as seaweed, invertebrates, microalgae, fungi, and bacteria have garnered growing attention due to their diverse bioactivities and potential therapeutic applications. Marine-derived FCCs characterized by high fucose residue content and extensive sulfate substitution, including fucoidan, fucosylated chondroitin sulfate, and fucose-rich microbial exopolysaccharides, have demonstrated significant potential in promoting gastrointestinal health. This review describes the unique structural features of FCCs and summarizes their health benefits, including regulation of gut microbiota, modulation of microbial metabolism, anti-adhesion activities against <i>H. pylori</i> and gut pathogens, protection against inflammatory injuries, and anti-tumor activities. Additionally, this review discusses the structural characteristics that influence the functional properties and the limitations related to the activity research and preparation processes of FCCs, providing a balanced perspective on the application potential and challenges of FCCs with specific structures for the regulation of gastrointestinal health and diseases.</p>","PeriodicalId":12386,"journal":{"name":"Foods","volume":"13 21","pages":""},"PeriodicalIF":4.7,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11545675/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anila Antony, Aswathi Soni, Linda M Samuelsson, Mike Weeks, Meng Wai Woo, Siew-Young Quek, Mohammed Farid, Tanushree Gupta
The efficacy of ultra-high-pressure homogenisation (UHPH) in inactivating Bacillus pumilus ATCC 27142 and Bacillus subtilis ATCC 6633 spores suspended in sheep and cow milk was investigated. The UHPH treatment was conducted at 200 and 250 MPa with an inlet temperature of 85 °C, resulting in homogenising valve temperatures of 117 °C and 127 °C, respectively. To isolate the role of temperature and pressure in the inactivation of bacterial spores, the UHPH treatment was repeated at 250 MPa with a lower inlet temperature of 70 °C that resulted in a valve temperature of 117 °C. Increasing the pressure and valve temperature resulted in increased inactivation. At 250 MPa with a valve temperature of 127 °C, greater than 5 log CFU/mL reduction was achieved in B. pumilus and B. subtilis spores in both milk types. Reductions of 0.61 ± 0.03 log CFU/mL and 0.62 ± 0.09 log CFU/mL in B. pumilus spores and 1.18 ± 0.04 log CFU/mL and 1.30 ± 0.07 log CFU/mL in B. subtilis spores were obtained at 250 MPa with a valve temperature of 117 °C in sheep and cow milk, respectively. The spore inactivation was influenced by both the pressure and temperature, suggesting a synergistic effect, with the latter playing a critical role in the lethality of the treatment. No significant differences in the inactivation of either strain was observed between sheep and cow milk.
{"title":"Impact of Ultra-High-Pressure Homogenisation on the Inactivation of <i>Bacillus pumilus</i> and <i>Bacillus subtilis</i> Spores in Sheep and Cow Milk.","authors":"Anila Antony, Aswathi Soni, Linda M Samuelsson, Mike Weeks, Meng Wai Woo, Siew-Young Quek, Mohammed Farid, Tanushree Gupta","doi":"10.3390/foods13213452","DOIUrl":"10.3390/foods13213452","url":null,"abstract":"<p><p>The efficacy of ultra-high-pressure homogenisation (UHPH) in inactivating <i>Bacillus pumilus</i> ATCC 27142 and <i>Bacillus subtilis</i> ATCC 6633 spores suspended in sheep and cow milk was investigated. The UHPH treatment was conducted at 200 and 250 MPa with an inlet temperature of 85 °C, resulting in homogenising valve temperatures of 117 °C and 127 °C, respectively. To isolate the role of temperature and pressure in the inactivation of bacterial spores, the UHPH treatment was repeated at 250 MPa with a lower inlet temperature of 70 °C that resulted in a valve temperature of 117 °C. Increasing the pressure and valve temperature resulted in increased inactivation. At 250 MPa with a valve temperature of 127 °C, greater than 5 log CFU/mL reduction was achieved in <i>B. pumilus</i> and <i>B. subtilis</i> spores in both milk types. Reductions of 0.61 ± 0.03 log CFU/mL and 0.62 ± 0.09 log CFU/mL in <i>B. pumilus</i> spores and 1.18 ± 0.04 log CFU/mL and 1.30 ± 0.07 log CFU/mL in <i>B. subtilis</i> spores were obtained at 250 MPa with a valve temperature of 117 °C in sheep and cow milk, respectively. The spore inactivation was influenced by both the pressure and temperature, suggesting a synergistic effect, with the latter playing a critical role in the lethality of the treatment. No significant differences in the inactivation of either strain was observed between sheep and cow milk.</p>","PeriodicalId":12386,"journal":{"name":"Foods","volume":"13 21","pages":""},"PeriodicalIF":4.7,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11545649/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142618001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}