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Nutritional Composition of Commercially Sourced Meat from Two Anatomical Locations Under Regenerative and Intensive Production Systems. 在再生和集约化生产系统下,来自两个解剖位置的商业肉类的营养成分。
IF 5.1 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-03-07 DOI: 10.3390/foods15050940
Irene C Antunes, Luísa Cristina Roseiro, Helena Gonçalves, Elsa M Gonçalves, Andreia Soares, Carla Alegria, Nuno Alvarenga, João Reis, Margarida Oliveira, Igor Dias

Regenerative agriculture approaches in livestock production may help produce animal protein that aligns with increasingly demanding sustainability criteria. This study compared commercially sourced beef from regenerative farming systems (RFS; n = 10; Longissimus lumborum, n = 5; Splenius capitis, n = 5) and intensive systems (IS; n = 6; Longissimus lumborum, n = 3; Splenius capitis, n = 3), evaluating the effects of production system (PS), muscle type (MT), and their interaction (MT × PS) on nutritional traits. IS chuck had higher polyunsaturated fatty acids (PUFA) and n-6 PUFA family (n-6 PUFA) contents (p < 0.05; 10.24 and 9.15 g fatty acids (FA)/100 g total FA, respectively), driven by C18:2 cis-9, trans-11, C20:4 n-6, and C18:2 n-6 contents. Consequently, IS chuck had a higher polyunsaturated FA and saturated FA ratio (P/S), peroxidability index (PI), n-6 PUFA family and n-3 PUFA family ratio (n-6/n-3), and hypocholesterolemic/hypercholesterolemic ratio (h/H) values (p < 0.05; 0.13, 23.87, 9.33 and 0.32, respectively). By comparison, RFS chuck had the highest n-3 PUFA content (p < 0.05; 1.28 g FA/100 g total FA), primarily due to its higher C18:3 n-3 content, resulting in a lower n-6/n-3 ratio (3.95). RFS meat showed higher vitamin E and α-tocopherol (0.58 and 0.56 mg/100 g of meat, respectively), exceeding the ≥0.30 mg/100 g threshold proposed to limit lipid oxidation, unlike IS meat.

畜牧业生产中的再生农业方法可能有助于生产符合日益严格的可持续性标准的动物蛋白。本研究比较了再生养殖系统(RFS, n = 10;腰最长肌,n = 5;头脾,n = 5)和集约化养殖系统(IS, n = 6;腰最长肌,n = 3;头脾,n = 3)的商业牛肉,评估了生产系统(PS)、肌肉类型(MT)及其相互作用(MT × PS)对营养性状的影响。IS卡盘多不饱和脂肪酸(PUFA)和n-6 PUFA家族(n-6 PUFA)含量较高(p < 0.05,分别为10.24和9.15 g脂肪酸(FA)/100 g总FA),这是由C18:2顺式-9、反式-11、C20:4 n-6和C18:2 n-6含量驱动的。因此,IS卡盘具有较高的多不饱和FA和饱和FA比值(P/S)、过氧化物指数(PI)、n-6 PUFA家族和n-3 PUFA家族比值(n-6/n-3)和低胆固醇/高胆固醇比值(h/ h)值(P < 0.05,分别为0.13、23.87、9.33和0.32)。相比之下,RFS卡盘的n-3 PUFA含量最高(p < 0.05,为1.28 g FA/100 g总FA),这主要是由于RFS卡盘的C18:3 n-3含量较高,导致n-6/n-3比较低(3.95)。与IS肉不同,RFS肉的维生素E和α-生育酚含量较高(分别为0.58和0.56 mg/100 g),超过了限制脂质氧化的≥0.30 mg/100 g阈值。
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引用次数: 0
Functional Additives Enhance Freeze-Thaw Stability and Retard Retrogradation in Wheat-Potato Starch Gels and Crystal Dumpling Wrappers. 功能添加剂提高小麦-马铃薯淀粉凝胶和晶体饺子皮的冻融稳定性和延缓变质。
IF 5.1 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-03-07 DOI: 10.3390/foods15050943
Yongmei Mo, Qingfei Duan, Fuhan Xie, Yujia Wei, Huabing Zhai, Shudan Tan, Fengwei Xie, Pei Chen

Crystal dumpling wrapper production is hampered by rapid surface dehydration, severe freeze-cracking propensity, and storage-induced retrogradation. Modulation of blended starch properties through functional additives was investigated. This study systematically evaluated the impact of hydroxypropyl distarch phosphate (HPDSP), trehalose (TRE), guar gum (GG), and composite phosphates (CP) on physicochemical and structural properties of wheat-potato starch composite gel. Concurrently, the effects of additives on the cracking rate of crystal dumplings and texture of wrappers were investigated. Analysis revealed that apparent viscosity was increased by all additives except CP. Different additives significantly improved the freeze-thaw stability of the composite gel during the first three cycles. GG maintained enhanced freeze-thaw stability throughout the entire freeze-thaw cycle (dehydration shrinkage rate: 2.69-40.55%). Multivariate analytical techniques (SEM, FTIR, XRD, DSC) collectively indicated that the additives effectively inhibited starch retrogradation, whilst HPDSP showed the strongest retrogradation inhibition. CP enhanced water-retention capacity and produced a softer blended gel (hardness at 21 days was 100.56 gf). Furthermore, additives significantly reduced the freezing cracking rate of crystal dumplings and improved the textural properties of dumpling wrappers.

晶体饺子皮的生产受到表面快速脱水、严重的冻裂倾向和储存引起的退化的阻碍。研究了功能性添加剂对共混淀粉性能的调节作用。本研究系统评价了羟丙基二淀粉磷酸(HPDSP)、海藻糖(TRE)、瓜尔胶(GG)和复合磷酸盐(CP)对小麦-马铃薯淀粉复合凝胶理化和结构性能的影响。同时,考察了添加剂对水饺脆裂率和饺子皮质地的影响。分析表明,除CP外,其他添加剂均显著提高了复合凝胶的表观粘度。在前3次循环中,不同添加剂显著提高了复合凝胶的冻融稳定性。GG在整个冻融循环中保持了较好的冻融稳定性(脱水收缩率为2.69 ~ 40.55%)。多变量分析技术(SEM, FTIR, XRD, DSC)共同表明添加剂能有效抑制淀粉的退化,其中HPDSP的抑制作用最强。CP增强了保水能力,并产生了更软的混合凝胶(21天的硬度为100.56 gf)。此外,添加剂显著降低了晶体饺子的冷冻开裂率,改善了饺子皮的结构性能。
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引用次数: 0
From Waste to Bioactive Ingredient: Integrated Extraction, Identification, and Validation of Novel Antioxidant Peptides from Xuefeng Black-Bone Chicken Bones 从废物到生物活性成分:雪峰乌骨鸡骨中新型抗氧化肽的综合提取、鉴定和验证
2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-03-07 DOI: 10.3390/foods15050942
Haige Yang, Fanjia Kong, Lan Mo, Yanyang Wu, Aihua Lou, Qingwu Shen, Wei Quan, Lei Zhou, Meichun Li, Yan Liu
The valorization of poultry bone by-products into high-value bioactive ingredients aligns with the principles of a sustainable circular bioeconomy. This study established an integrated process for the production, identification, and validation of bioactive antioxidant peptides from Xuefeng black-bone chicken bones (BCB). Alcalase was selected as the optimal protease due to its superior performance in both the degree of hydrolysis and antioxidant activity under the optimized conditions. Using response surface methodology (RSM), the optimal hydrolysis conditions were determined as 50 °C, pH 10.18, and 4.2 h, resulting in a hydrolysate with a hydrolysis degree of 25.10% and ABTS radical scavenging activity of 84.36%. Upon ultrafiltration, the <3 kDa fraction demonstrated a significantly higher antioxidant capacity than the crude hydrolysate. Further purification through gel filtration chromatography yielded the F3 sub-fraction (predominantly <1 kDa peptides), which exhibited the most potent activity across all four antioxidant assays conducted (ABTS, DPPH, hydroxyl radical scavenging, and reducing power). A liquid chromatography–tandem mass spectrometry (LC-MS/MS) analysis of F3 led to the identification of 21 peptide sequences. An in silico screening based on bioactivity and toxicity predictions pinpointed three promising candidates: DYPF, WDY, and FGYK. These peptides were chemically synthesized and validated to possess significant in vitro radical scavenging activities against both DPPH and hydroxyl radicals. Molecular docking simulations revealed that all three peptides could spontaneously bind to the Keap1 protein with a high affinity (binding energy < −7.0 kcal/mol), primarily through hydrogen bonds and hydrophobic interactions, suggesting a possible molecular mechanism that may involve the Keap1-Nrf2-ARE antioxidant pathway. This computational insight provides a testable hypothesis for their bioactivity, the verification of which is contingent upon future studies demonstrating their cellular delivery and intracellular action. This work not only provides a sustainable strategy for BCB utilization but also identifies potent antioxidant peptides with potential applications in functional foods and nutraceuticals.
将家禽骨骼副产品转化为高价值的生物活性成分符合可持续循环生物经济的原则。本研究建立了雪峰乌骨鸡骨(BCB)生物活性抗氧化肽的生产、鉴定和验证的综合工艺。在优化条件下,Alcalase在水解程度和抗氧化活性方面均表现优异,被选为最佳蛋白酶。采用响应面法(RSM)确定最佳水解条件为50°C, pH 10.18, 4.2 h,水解度为25.10%,ABTS自由基清除活性为84.36%。超滤后,3 kDa馏分的抗氧化能力明显高于粗水解产物。通过凝胶过滤层析进一步纯化得到F3亚部分(主要是1 kDa肽),在所有四种抗氧化实验(ABTS, DPPH,羟基自由基清除和还原能力)中表现出最有效的活性。对F3进行液相色谱-串联质谱(LC-MS/MS)分析,鉴定出21个肽序列。一项基于生物活性和毒性预测的计算机筛选确定了三种有希望的候选药物:DYPF、WDY和FGYK。这些肽是化学合成的,并被证实具有显著的体外自由基清除活性,对DPPH和羟基自由基。分子对接模拟显示,这三种肽均能以高亲和力(结合能<;−7.0 kcal/mol)自发结合Keap1蛋白,主要通过氢键和疏水相互作用,提示可能涉及Keap1- nrf2 - are抗氧化途径的分子机制。这种计算洞察力为它们的生物活性提供了一个可测试的假设,其验证取决于未来的研究,证明它们的细胞传递和细胞内作用。这项工作不仅为BCB的可持续利用提供了策略,而且还确定了在功能食品和营养保健品中具有潜在应用价值的有效抗氧化肽。
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引用次数: 0
Microwave Pretreatment of Peanuts Modulates Oil Body Emulsion Stability: Mechanism and Application as a Source Modification Strategy for Efficient Demulsification. 花生微波预处理对油体乳剂稳定性的调节:机理及作为高效破乳源改性策略的应用。
IF 5.1 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-03-07 DOI: 10.3390/foods15050951
Nan Hai, Fusheng Chen

This study investigated microwave pretreatment (0-900 W) of peanuts as a source modification strategy to reduce the stability of peanut oil body emulsions (POBEs) and improve aqueous enzymatic extraction. Results indicated that higher power treatment (≥540 W) significantly destabilized POBE. The optimal condition at 720 W increased POBE extraction yield and demulsification rate by 16.82% and 46.32%, respectively, compared with the control. This destabilization was attributed to marked changes in interfacial properties, including decreased apparent viscosity, lowered absolute ζ-potential (from 35.93 mV to 27.09 mV), increased particle size (from 1177.16 nm to 1976.98 nm), and the microstructure of droplet aggregation. Compositional analysis revealed that microwave treatment induced POBE reorganization, characterized by increased lipid and phospholipid contents alongside reduced moisture, solid, and protein levels. Further interfacial protein analysis revealed that exposure triggered protein conformational unfolding, hydrophobic group exposure, and subsequent aggregation, which weakened protein adsorption at the interface and reduced the mechanical strength of the interfacial film. These findings elucidate the mechanism of microwave-induced emulsion instability, providing a theoretical basis for enhancing oil extraction efficiency through raw material pretreatment.

本研究研究了花生微波预处理(0-900 W)作为源改性策略,以降低花生油体乳(POBEs)的稳定性并改善水酶提取。结果表明,高功率处理(≥540 W)显著破坏了POBE的稳定性。在720 W的最佳条件下,POBE提取率和破乳率分别比对照提高了16.82%和46.32%。这种失稳是由于界面性能的显著变化,包括表观粘度的降低、绝对ζ电位的降低(从35.93 mV到27.09 mV)、颗粒尺寸的增加(从1177.16 nm到1976.98 nm)以及液滴聚集的微观结构。成分分析表明,微波处理诱导POBE重组,其特征是脂质和磷脂含量增加,水分、固体和蛋白质含量降低。进一步的界面蛋白分析表明,暴露引发了蛋白质构象展开、疏水性基团暴露和随后的聚集,从而削弱了蛋白质在界面上的吸附,降低了界面膜的机械强度。这些研究结果阐明了微波诱导乳化液不稳定的机理,为通过原料预处理提高萃取效率提供了理论依据。
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引用次数: 0
Correlations Between Sensory Evaluations and Instrumental Measurements in Milk Chocolate with Varying Emulsifier Levels and Particle Sizes. 不同乳化剂水平和粒度下牛奶巧克力感官评价与仪器测量的相关性。
IF 5.1 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-03-07 DOI: 10.3390/foods15050938
Burcu Sasmaz, Gurbuz Gunes

This study was conducted to investigate and identify correlations among sensory and comprehensive consumer test results with rheological, textural, and tribological properties of milk chocolate in response to varying levels of particle size and emulsifier. To simulate realistic oral conditions, artificial saliva was incorporated into instrumental analyses. Rheological analysis revealed that increasing particle size and emulsifier concentration significantly reduced plastic viscosity, while emulsifier concentration alone increased yield stress due to structural reorganization within the fat phase. Tribological measurements demonstrated that larger particles increased friction in boundary and mixed lubrication regimes, whereas emulsifiers reduced friction in these regimes by enhancing fluid film formation. Under elastohydrodynamic conditions and with artificial saliva, friction was more influenced by the interaction between particle size and emulsifier level. Textural analysis showed that both parameters significantly influenced hardness, with saliva further softening the samples, especially those with higher emulsifier levels. Sensory evaluations indicated that emulsifiers enhanced flavor release and mouthfeel attributes, while smaller particles contributed to smoother texture and more balanced flavor perception. Consumer acceptance tests confirmed that samples with smaller particles and higher emulsifier levels received the highest scores in overall liking, taste, and texture. Instrumental parameters strongly correlated with key sensory attributes, with yield stress showing the highest positive associations with creaminess, smoothness, fat/milk flavor, and liking, while higher viscosity and friction were negatively linked to flavor release and mouthfeel. Instrumental hardness negatively correlated with cacao intensity and astringency, while saliva-induced softening was positively associated with sweetness and liking, highlighting the role of dynamic oral softening.

本研究旨在调查和确定牛奶巧克力在不同粒度和乳化剂水平下的流变学、质地和摩擦学性能与感官和综合消费者测试结果之间的相关性。为了模拟真实的口腔状况,人工唾液被纳入仪器分析。流变学分析表明,增加颗粒尺寸和乳化剂浓度可显著降低塑料粘度,而乳化剂浓度单独增加由于脂肪相内部结构重组导致的屈服应力。摩擦学测量表明,在边界润滑和混合润滑中,较大的颗粒会增加摩擦,而乳化剂通过促进流体膜的形成来减少摩擦。在有人工唾液的弹性流体条件下,颗粒大小和乳化剂水平的相互作用对摩擦的影响更大。结构分析表明,这两个参数对样品的硬度都有显著影响,唾液进一步软化了样品,尤其是乳化剂含量较高的样品。感官评价表明,乳化剂增强了风味释放和口感属性,而更小的颗粒有助于更光滑的质地和更平衡的风味感知。消费者接受度测试证实,颗粒较小、乳化剂含量较高的样品在总体喜欢度、味道和质地方面得分最高。仪器参数与关键感官属性密切相关,其中屈服应力与奶油度、顺滑度、脂肪/牛奶风味和喜爱度呈正相关,而较高的粘度和摩擦力与风味释放和口感负相关。仪器硬度与可可强度和涩味呈负相关,而唾液诱导的软化与甜味和喜欢度呈正相关,突出了动态口腔软化的作用。
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引用次数: 0
Shelf Life Extension of Fresh Buffalo Meat Using Spice Powders and Lavender Essential Oil During Storage Under Refrigeration. 在冷藏条件下使用香料粉和薰衣草精油延长新鲜水牛肉的保质期。
IF 5.1 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-03-07 DOI: 10.3390/foods15050947
Athanasia P Marangeli, Vassilios K Karabagias, Glykeria E Angelaki, Dimitrios G Lazaridis, Nikolaos D Andritsos, Olga Malisova, Ioannis K Karabagias

We studied the shelf life of fresh buffalo meat in polyamide/polyethylene (PA/PE) packaging during refrigerated storage for 14 days, when treated with cinnamon-clove (C-C) and nutmeg (Nut) powders, along with lavender essential oil (LEO). Microbiological (total viable count, Pseudomonas spp., Brochothrix thermosphacta, Enterobacteriaceae, and lactic acid bacteria), antibacterial (Salmonella Typhimurium and Staphylococcus aureus), physicochemical and biochemical (pH, moisture, color, total fat, hemoglobin and heme iron, 2-thiobarbituric acid, mercaptans, antioxidant activity, and total phenolic content), and sensory (color, odor, texture, and taste) analyses were carried out. The results showed that C-C and Nut powder extracts exhibited significant (p < 0.05) antioxidant and antibacterial activity, higher than LEO; however, all treatments delayed lipid oxidation. Based primarily on sensory evaluation, the shelf life extension of buffalo meat was 2-3 days for LEO and Nut powder, and 4-6 days for C-C powder. Factor analysis indicated the critical days of refrigerated storage for the evolution of spoilage-related biochemical parameters.

我们研究了用肉桂丁香(C-C)和肉豆蔻(Nut)粉末以及薰衣草精油(LEO)处理的新鲜水牛肉在聚酰胺/聚乙烯(PA/PE)包装下冷藏14天的保质期。进行微生物学(总活菌数、假单胞菌、热嗜菌、肠杆菌科和乳酸菌)、抗菌(鼠伤寒沙门氏菌和金黄色葡萄球菌)、理化生化(pH、水分、颜色、总脂肪、血红蛋白和血红素铁、2-硫代巴比托酸、硫醇、抗氧化活性和总酚含量)和感官(颜色、气味、质地和味道)分析。结果表明,C-C和坚果粉提取物具有显著(p < 0.05)的抗氧化和抗菌活性,均高于LEO;然而,所有的治疗都延迟了脂质氧化。以感官评价为主,LEO和坚果粉的水牛肉保质期延长2-3天,C-C粉的水牛肉保质期延长4-6天。因子分析显示了腐坏相关生化参数变化的关键冷藏天数。
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引用次数: 0
Mechanistic Insights into the Cooperative Removal of NH3 and H2S by Persimmon Polyphenols with Natural Deep Eutectic Solvent Systems 柿子多酚与天然深共晶溶剂体系协同去除NH3和H2S的机理研究
2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-03-07 DOI: 10.3390/foods15050939
Baixue Li, Lu Li, Qingyun Guan, Chunmei Li
Persimmon polyphenols (PP) are natural polyphenols with high reactivity and strong deodorization potential; however, their practical application in odor control is limited by their poor solubility. In this study, natural deep eutectic solvents (NADESs) were employed for the green extraction of PP, and the capabilities of extracts on the removal of ammonia (NH3) and hydrogen sulfide (H2S) were investigated. In addition, the underlying mechanisms were explored by integrating spectroscopic analysis, molecular dynamics simulations, and quantum chemical calculations. The results showed that chloride-citric acid (CC-CA) was the optimal system in both PP extraction and sustained NH3 removal, while the betaine-urea (B-U) system was more effective for H2S removal. NH3 removal was governed by acid-base neutralization, with the resulting ammonium species being further stabilized within the PP-regulated NADES hydrogen-bond network. In contrast, H2S interacted with the solvent network not only through acid-base neutralization but also via Van der Waals forces and hydrophobic contacts. Our data supported that NADESs enhanced the deodorization performance of PP through cooperative microenvironment regulation rather than irreversible chemical conversion. This work highlighted that NADESs could not only function as highly efficient extraction media for polyphenols, but also active platforms for enhancing selective gas-capture capability for polyphenols. Furthermore, it provided a new strategy for the rational design of green, persimmon-derived deodorants.
柿子多酚(PP)是一种反应活性高、脱臭潜力强的天然多酚类物质;然而,它们在气味控制方面的实际应用受到其溶解度差的限制。本研究采用天然深共晶溶剂(NADESs)对PP进行绿色萃取,考察了萃取物对氨(NH3)和硫化氢(H2S)的去除能力。此外,通过光谱分析、分子动力学模拟和量子化学计算,探讨了潜在的机制。结果表明,氯化物-柠檬酸(CC-CA)体系对PP的提取和NH3的持续去除效果最佳,甜菜碱-尿素(B-U)体系对H2S的去除效果更好。NH3的去除由酸碱中和控制,产生的铵态物质在pp调节的NADES氢键网络中进一步稳定。相比之下,H2S不仅通过酸碱中和作用,还通过范德华力和疏水接触与溶剂网络相互作用。我们的数据支持NADESs通过协同微环境调节而不是不可逆的化学转化来增强PP的脱臭性能。研究结果表明,NADESs不仅可以作为多酚类物质的高效提取介质,而且可以作为提高多酚类物质选择性气体捕获能力的活性平台。为绿色柿子除臭剂的合理设计提供了新的思路。
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引用次数: 0
Babassu (Attalea speciosa) Mesocarp Flour Extract Inhibits Lipid Peroxidation and Pro-Oxidant Enzymes: In Vitro and In Silico Evidence. 巴巴苏(Attalea speciosa)中果皮面粉提取物抑制脂质过氧化和促氧化酶:体外和室内证据。
IF 5.1 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-03-07 DOI: 10.3390/foods15050945
Caroline Chavier Pereira Santana, Fernanda Farias Costa, Jaqueline Daniele Santos Barros, Michelli Erica Souza Ferreira, Richard Pereira Dutra, Antônio Silva Machado, Aramys Silva Reis

(1) Background: Babassu (Attalea speciosa) mesocarp is a functional food rich in nutrients and phenolic compounds, offering antioxidant and metabolic benefits. However, its effects on lipid peroxidation and pro-oxidant enzymes remain poorly explored. (2) Methods: The antioxidant potential of a hydroalcoholic extract of babassu mesocarp (HEB) was assessed using DPPH radical scavenging and lipid peroxidation inhibition, measured by the TBARS assay. Cytotoxicity was assessed by the MTT assay. Molecular docking was conducted to investigate interactions between HEB-derived compounds and NADPH oxidase and xanthine oxidase. (3) Results: HEB showed dose-dependent antioxidant activity (IC50 = 4.734 µg/mL) and effectively inhibited lipid peroxidation (IC50 = 51.35 µg/mL), with no cytotoxic effects. In silico analyses suggested potential inhibition of pro-oxidant enzymes. (4) Conclusions: HEB exhibits a strong ability to inhibit lipid peroxidation and theoretical enzyme-inhibitory potential, supporting its use in functional foods and nutraceuticals.

(1)背景:巴巴苏(Attalea speciosa)中果皮是一种富含营养物质和酚类化合物的功能性食品,具有抗氧化和代谢作用。然而,其对脂质过氧化和促氧化酶的影响仍然知之甚少。(2)方法:采用TBARS法测定巴巴苏中果皮水醇提取物(HEB)对DPPH自由基的清除能力和对脂质过氧化的抑制能力,评估其抗氧化能力。MTT法测定细胞毒性。通过分子对接研究了heb衍生化合物与NADPH氧化酶和黄嘌呤氧化酶的相互作用。(3)结果:HEB具有剂量依赖性抗氧化活性(IC50 = 4.734µg/mL),有效抑制脂质过氧化(IC50 = 51.35µg/mL),无细胞毒性作用。硅分析表明可能抑制促氧化酶。(4)结论:HEB具有较强的抑制脂质过氧化的能力和理论上的酶抑制潜力,支持其在功能食品和营养保健品中的应用。
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引用次数: 0
Class-Specific Immunochromatographic Assay Enabled by Mesoporous Nanozyme-Catalyzed Signal Amplification for On-Site Screening of Sulfonylureas. 基于介孔纳米酶催化信号放大的类特异性免疫层析分析用于磺胺脲类化合物的现场筛选。
IF 5.1 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-03-07 DOI: 10.3390/foods15050944
Yanting Li, Zixian He, Pengjie He, Zixuan Tang, Esra Bağda, Efkan Bağda, Zhenlin Xu, Xiangmei Li

Conventional immunochromatographic assays (ICAs) face limitations in sensitivity and dynamic range, hindering their application in on-site, class-specific screening of sulfonylurea (SU) adulteration in functional foods. To address this, a signal amplification strategy was developed by engineering high-density platinum nanozymes on a mesoporous metal-organic framework (PCN-224). The mesoporous architecture of PCN-224 facilitated high-density and stable loading of catalytically active Pt sites. The established PCN-224@Pt-based ICA achieved detection limits of 0.52-7.94 μg/kg in tea and 0.69-7.02 μg/kg in capsules, with linear ranges of 1.69-513.01 μg/kg and 2.05-716.47 μg/kg, respectively. Compared with traditional colloidal gold immunochromatographic assays (CG-ICAs), sensitivity was improved by up to 57-fold, while the linear detection range was expanded by over 5-fold relative to the previously reported PCN-224@PDA- ICA. The method demonstrated recovery rates of 81.8-119.8% and coefficients of variation between 2.5% and 11.4%. Validation against LC-MS/MS using 20 real samples showed excellent agreement (R2 > 0.99). This work not only provides a sensitive and rapid tool for the surveillance of SU adulteration in functional foods but also establishes a generalizable nanozyme design strategy applicable to enhancing the performance of a wide range of ICA-based detection platforms.

传统的免疫层析分析方法在灵敏度和动态范围上存在局限性,阻碍了其在功能食品中磺酰脲(SU)掺假的现场、类别特异性筛选中的应用。为了解决这一问题,通过在介孔金属有机骨架(PCN-224)上设计高密度铂纳米酶,开发了一种信号放大策略。PCN-224的介孔结构有利于高密度和稳定的负载催化活性Pt位点。建立的PCN-224@Pt-based ICA在茶叶和胶囊中的检出限分别为0.52 ~ 7.94 μg/kg和0.69 ~ 7.02 μg/kg,线性范围分别为1.69 ~ 513.01 μg/kg和2.05 ~ 716.47 μg/kg。与传统胶体金免疫层析法(CG-ICAs)相比,灵敏度提高了57倍,而线性检测范围比先前报道的PCN-224@PDA- ICA扩大了5倍以上。回收率为81.8 ~ 119.8%,变异系数为2.5% ~ 11.4%。使用20个真实样品对LC-MS/MS进行验证,结果一致(R2 > 0.99)。这项工作不仅为功能食品中SU掺假的监测提供了一种灵敏、快速的工具,而且还建立了一种通用的纳米酶设计策略,适用于提高各种基于ica的检测平台的性能。
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引用次数: 0
Effects of Salt-Reducing Alternatives on the Oral Processing Characteristics of Chickpea Nang. 降盐替代品对鹰嘴豆嘴加工特性的影响。
IF 5.1 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-03-07 DOI: 10.3390/foods15050941
Qian Wang, Ying Li, Sailimuhan Asimi

Salt reduction is an important strategy for healthy diets. Our previous study developed low-sodium chickpea nang (LCHN) using potassium chloride, wheat gluten, inulin and L-lysine. However, consumers also value taste. The impact of this reformulation on oral processing characteristics remains unclear. This study collected chewing samples from 12 volunteers at five mastication stages (0%, 25%, 50%, 75%, and 100%) of regular chickpea nang (CHN) and LCHN, measuring chewing parameters, bolus moisture content, saliva addition amount, and flow rate. Results indicated that LCHN had a significantly shorter swallowing time (24.22 ± 3.63 s vs. 27.84 ± 6.01 s, p < 0.05, Cohen's d = 0.73), while the number of chews (Nc), chewing frequency (Fc), bolus moisture content, and saliva flow rate showed no inter-group differences across all mastication stages (p > 0.05). Bolus moisture content increased significantly with mastication progression in both groups (p < 0.05), whereas saliva addition amount and flow rate decreased significantly (p < 0.05). Additionally, higher chewing frequency correlated with increased saliva addition amount and reduced flow rate (p < 0.05). In CHN, the Nc positively correlated with chewing time (r = 0.452, p < 0.01) and frequency (r = 0.458, p < 0.01), whereas in LCHN it negatively correlated with time (r = -0.329, p < 0.05) and positively with frequency (r = 0.884, p < 0.01). These findings provide theoretical basis for low-sodium baked product development.

减少食盐是健康饮食的一项重要策略。本研究利用氯化钾、小麦面筋、菊粉和l -赖氨酸制备了低钠鹰嘴豆粕。然而,消费者也重视口味。这种重新配方对口腔加工特征的影响尚不清楚。本研究采集了12名志愿者在普通鹰嘴豆(CHN)和LCHN五个咀嚼阶段(0%、25%、50%、75%和100%)的咀嚼样本,测量了咀嚼参数、丸含水量、唾液添加量和流速。结果显示,LCHN大鼠吞咽时间(24.22±3.63 s vs. 27.84±6.01 s, p < 0.05, Cohen’s d = 0.73)明显短于LCHN大鼠(24.22±3.63 s vs. 27.84±6.01 s),各组咀嚼次数(Nc)、咀嚼频率(Fc)、颗粒水分含量、唾液流率在各咀嚼阶段无组间差异(p < 0.05)。随着咀嚼的进行,两组动物的颗粒水分含量均显著增加(p < 0.05),唾液添加量和流速均显著降低(p < 0.05)。咀嚼频率越高,唾液添加量越高,唾液流率越低(p < 0.05)。CHN与咀嚼时间(r = 0.452, p < 0.01)、咀嚼频率(r = 0.458, p < 0.01)呈正相关,LCHN与咀嚼时间(r = -0.329, p < 0.05)负相关,与咀嚼频率(r = 0.884, p < 0.01)正相关。研究结果为低钠烘焙产品的开发提供了理论依据。
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