Irene C Antunes, Luísa Cristina Roseiro, Helena Gonçalves, Elsa M Gonçalves, Andreia Soares, Carla Alegria, Nuno Alvarenga, João Reis, Margarida Oliveira, Igor Dias
Regenerative agriculture approaches in livestock production may help produce animal protein that aligns with increasingly demanding sustainability criteria. This study compared commercially sourced beef from regenerative farming systems (RFS; n = 10; Longissimus lumborum, n = 5; Splenius capitis, n = 5) and intensive systems (IS; n = 6; Longissimus lumborum, n = 3; Splenius capitis, n = 3), evaluating the effects of production system (PS), muscle type (MT), and their interaction (MT × PS) on nutritional traits. IS chuck had higher polyunsaturated fatty acids (PUFA) and n-6 PUFA family (n-6 PUFA) contents (p < 0.05; 10.24 and 9.15 g fatty acids (FA)/100 g total FA, respectively), driven by C18:2 cis-9, trans-11, C20:4 n-6, and C18:2 n-6 contents. Consequently, IS chuck had a higher polyunsaturated FA and saturated FA ratio (P/S), peroxidability index (PI), n-6 PUFA family and n-3 PUFA family ratio (n-6/n-3), and hypocholesterolemic/hypercholesterolemic ratio (h/H) values (p < 0.05; 0.13, 23.87, 9.33 and 0.32, respectively). By comparison, RFS chuck had the highest n-3 PUFA content (p < 0.05; 1.28 g FA/100 g total FA), primarily due to its higher C18:3 n-3 content, resulting in a lower n-6/n-3 ratio (3.95). RFS meat showed higher vitamin E and α-tocopherol (0.58 and 0.56 mg/100 g of meat, respectively), exceeding the ≥0.30 mg/100 g threshold proposed to limit lipid oxidation, unlike IS meat.
{"title":"Nutritional Composition of Commercially Sourced Meat from Two Anatomical Locations Under Regenerative and Intensive Production Systems.","authors":"Irene C Antunes, Luísa Cristina Roseiro, Helena Gonçalves, Elsa M Gonçalves, Andreia Soares, Carla Alegria, Nuno Alvarenga, João Reis, Margarida Oliveira, Igor Dias","doi":"10.3390/foods15050940","DOIUrl":"10.3390/foods15050940","url":null,"abstract":"<p><p>Regenerative agriculture approaches in livestock production may help produce animal protein that aligns with increasingly demanding sustainability criteria. This study compared commercially sourced beef from regenerative farming systems (RFS; <i>n</i> = 10; <i>Longissimus lumborum</i>, <i>n</i> = 5; <i>Splenius capitis</i>, <i>n</i> = 5) and intensive systems (IS; <i>n</i> = 6; <i>Longissimus lumborum</i>, <i>n</i> = 3; <i>Splenius capitis</i>, <i>n</i> = 3), evaluating the effects of production system (PS), muscle type (MT), and their interaction (MT × PS) on nutritional traits. IS chuck had higher polyunsaturated fatty acids (PUFA) and n-6 PUFA family (n-6 PUFA) contents (<i>p</i> < 0.05; 10.24 and 9.15 g fatty acids (FA)/100 g total FA, respectively), driven by C18:2 <i>cis</i>-9, <i>trans</i>-11, C20:4 n-6, and C18:2 n-6 contents. Consequently, IS chuck had a higher polyunsaturated FA and saturated FA ratio (P/S), peroxidability index (PI), n-6 PUFA family and n-3 PUFA family ratio (n-6/n-3), and hypocholesterolemic/hypercholesterolemic ratio (h/H) values (<i>p</i> < 0.05; 0.13, 23.87, 9.33 and 0.32, respectively). By comparison, RFS chuck had the highest n-3 PUFA content (<i>p</i> < 0.05; 1.28 g FA/100 g total FA), primarily due to its higher C18:3 n-3 content, resulting in a lower n-6/n-3 ratio (3.95). RFS meat showed higher vitamin E and α-tocopherol (0.58 and 0.56 mg/100 g of meat, respectively), exceeding the ≥0.30 mg/100 g threshold proposed to limit lipid oxidation, unlike IS meat.</p>","PeriodicalId":12386,"journal":{"name":"Foods","volume":"15 5","pages":""},"PeriodicalIF":5.1,"publicationDate":"2026-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12984661/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147456622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Crystal dumpling wrapper production is hampered by rapid surface dehydration, severe freeze-cracking propensity, and storage-induced retrogradation. Modulation of blended starch properties through functional additives was investigated. This study systematically evaluated the impact of hydroxypropyl distarch phosphate (HPDSP), trehalose (TRE), guar gum (GG), and composite phosphates (CP) on physicochemical and structural properties of wheat-potato starch composite gel. Concurrently, the effects of additives on the cracking rate of crystal dumplings and texture of wrappers were investigated. Analysis revealed that apparent viscosity was increased by all additives except CP. Different additives significantly improved the freeze-thaw stability of the composite gel during the first three cycles. GG maintained enhanced freeze-thaw stability throughout the entire freeze-thaw cycle (dehydration shrinkage rate: 2.69-40.55%). Multivariate analytical techniques (SEM, FTIR, XRD, DSC) collectively indicated that the additives effectively inhibited starch retrogradation, whilst HPDSP showed the strongest retrogradation inhibition. CP enhanced water-retention capacity and produced a softer blended gel (hardness at 21 days was 100.56 gf). Furthermore, additives significantly reduced the freezing cracking rate of crystal dumplings and improved the textural properties of dumpling wrappers.
{"title":"Functional Additives Enhance Freeze-Thaw Stability and Retard Retrogradation in Wheat-Potato Starch Gels and Crystal Dumpling Wrappers.","authors":"Yongmei Mo, Qingfei Duan, Fuhan Xie, Yujia Wei, Huabing Zhai, Shudan Tan, Fengwei Xie, Pei Chen","doi":"10.3390/foods15050943","DOIUrl":"10.3390/foods15050943","url":null,"abstract":"<p><p>Crystal dumpling wrapper production is hampered by rapid surface dehydration, severe freeze-cracking propensity, and storage-induced retrogradation. Modulation of blended starch properties through functional additives was investigated. This study systematically evaluated the impact of hydroxypropyl distarch phosphate (HPDSP), trehalose (TRE), guar gum (GG), and composite phosphates (CP) on physicochemical and structural properties of wheat-potato starch composite gel. Concurrently, the effects of additives on the cracking rate of crystal dumplings and texture of wrappers were investigated. Analysis revealed that apparent viscosity was increased by all additives except CP. Different additives significantly improved the freeze-thaw stability of the composite gel during the first three cycles. GG maintained enhanced freeze-thaw stability throughout the entire freeze-thaw cycle (dehydration shrinkage rate: 2.69-40.55%). Multivariate analytical techniques (SEM, FTIR, XRD, DSC) collectively indicated that the additives effectively inhibited starch retrogradation, whilst HPDSP showed the strongest retrogradation inhibition. CP enhanced water-retention capacity and produced a softer blended gel (hardness at 21 days was 100.56 gf). Furthermore, additives significantly reduced the freezing cracking rate of crystal dumplings and improved the textural properties of dumpling wrappers.</p>","PeriodicalId":12386,"journal":{"name":"Foods","volume":"15 5","pages":""},"PeriodicalIF":5.1,"publicationDate":"2026-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12984116/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147456687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Haige Yang, Fanjia Kong, Lan Mo, Yanyang Wu, Aihua Lou, Qingwu Shen, Wei Quan, Lei Zhou, Meichun Li, Yan Liu
The valorization of poultry bone by-products into high-value bioactive ingredients aligns with the principles of a sustainable circular bioeconomy. This study established an integrated process for the production, identification, and validation of bioactive antioxidant peptides from Xuefeng black-bone chicken bones (BCB). Alcalase was selected as the optimal protease due to its superior performance in both the degree of hydrolysis and antioxidant activity under the optimized conditions. Using response surface methodology (RSM), the optimal hydrolysis conditions were determined as 50 °C, pH 10.18, and 4.2 h, resulting in a hydrolysate with a hydrolysis degree of 25.10% and ABTS radical scavenging activity of 84.36%. Upon ultrafiltration, the <3 kDa fraction demonstrated a significantly higher antioxidant capacity than the crude hydrolysate. Further purification through gel filtration chromatography yielded the F3 sub-fraction (predominantly <1 kDa peptides), which exhibited the most potent activity across all four antioxidant assays conducted (ABTS, DPPH, hydroxyl radical scavenging, and reducing power). A liquid chromatography–tandem mass spectrometry (LC-MS/MS) analysis of F3 led to the identification of 21 peptide sequences. An in silico screening based on bioactivity and toxicity predictions pinpointed three promising candidates: DYPF, WDY, and FGYK. These peptides were chemically synthesized and validated to possess significant in vitro radical scavenging activities against both DPPH and hydroxyl radicals. Molecular docking simulations revealed that all three peptides could spontaneously bind to the Keap1 protein with a high affinity (binding energy < −7.0 kcal/mol), primarily through hydrogen bonds and hydrophobic interactions, suggesting a possible molecular mechanism that may involve the Keap1-Nrf2-ARE antioxidant pathway. This computational insight provides a testable hypothesis for their bioactivity, the verification of which is contingent upon future studies demonstrating their cellular delivery and intracellular action. This work not only provides a sustainable strategy for BCB utilization but also identifies potent antioxidant peptides with potential applications in functional foods and nutraceuticals.
{"title":"From Waste to Bioactive Ingredient: Integrated Extraction, Identification, and Validation of Novel Antioxidant Peptides from Xuefeng Black-Bone Chicken Bones","authors":"Haige Yang, Fanjia Kong, Lan Mo, Yanyang Wu, Aihua Lou, Qingwu Shen, Wei Quan, Lei Zhou, Meichun Li, Yan Liu","doi":"10.3390/foods15050942","DOIUrl":"https://doi.org/10.3390/foods15050942","url":null,"abstract":"The valorization of poultry bone by-products into high-value bioactive ingredients aligns with the principles of a sustainable circular bioeconomy. This study established an integrated process for the production, identification, and validation of bioactive antioxidant peptides from Xuefeng black-bone chicken bones (BCB). Alcalase was selected as the optimal protease due to its superior performance in both the degree of hydrolysis and antioxidant activity under the optimized conditions. Using response surface methodology (RSM), the optimal hydrolysis conditions were determined as 50 °C, pH 10.18, and 4.2 h, resulting in a hydrolysate with a hydrolysis degree of 25.10% and ABTS radical scavenging activity of 84.36%. Upon ultrafiltration, the <3 kDa fraction demonstrated a significantly higher antioxidant capacity than the crude hydrolysate. Further purification through gel filtration chromatography yielded the F3 sub-fraction (predominantly <1 kDa peptides), which exhibited the most potent activity across all four antioxidant assays conducted (ABTS, DPPH, hydroxyl radical scavenging, and reducing power). A liquid chromatography–tandem mass spectrometry (LC-MS/MS) analysis of F3 led to the identification of 21 peptide sequences. An in silico screening based on bioactivity and toxicity predictions pinpointed three promising candidates: DYPF, WDY, and FGYK. These peptides were chemically synthesized and validated to possess significant in vitro radical scavenging activities against both DPPH and hydroxyl radicals. Molecular docking simulations revealed that all three peptides could spontaneously bind to the Keap1 protein with a high affinity (binding energy < −7.0 kcal/mol), primarily through hydrogen bonds and hydrophobic interactions, suggesting a possible molecular mechanism that may involve the Keap1-Nrf2-ARE antioxidant pathway. This computational insight provides a testable hypothesis for their bioactivity, the verification of which is contingent upon future studies demonstrating their cellular delivery and intracellular action. This work not only provides a sustainable strategy for BCB utilization but also identifies potent antioxidant peptides with potential applications in functional foods and nutraceuticals.","PeriodicalId":12386,"journal":{"name":"Foods","volume":"15 5","pages":"942-942"},"PeriodicalIF":0.0,"publicationDate":"2026-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147381839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study investigated microwave pretreatment (0-900 W) of peanuts as a source modification strategy to reduce the stability of peanut oil body emulsions (POBEs) and improve aqueous enzymatic extraction. Results indicated that higher power treatment (≥540 W) significantly destabilized POBE. The optimal condition at 720 W increased POBE extraction yield and demulsification rate by 16.82% and 46.32%, respectively, compared with the control. This destabilization was attributed to marked changes in interfacial properties, including decreased apparent viscosity, lowered absolute ζ-potential (from 35.93 mV to 27.09 mV), increased particle size (from 1177.16 nm to 1976.98 nm), and the microstructure of droplet aggregation. Compositional analysis revealed that microwave treatment induced POBE reorganization, characterized by increased lipid and phospholipid contents alongside reduced moisture, solid, and protein levels. Further interfacial protein analysis revealed that exposure triggered protein conformational unfolding, hydrophobic group exposure, and subsequent aggregation, which weakened protein adsorption at the interface and reduced the mechanical strength of the interfacial film. These findings elucidate the mechanism of microwave-induced emulsion instability, providing a theoretical basis for enhancing oil extraction efficiency through raw material pretreatment.
{"title":"Microwave Pretreatment of Peanuts Modulates Oil Body Emulsion Stability: Mechanism and Application as a Source Modification Strategy for Efficient Demulsification.","authors":"Nan Hai, Fusheng Chen","doi":"10.3390/foods15050951","DOIUrl":"10.3390/foods15050951","url":null,"abstract":"<p><p>This study investigated microwave pretreatment (0-900 W) of peanuts as a source modification strategy to reduce the stability of peanut oil body emulsions (POBEs) and improve aqueous enzymatic extraction. Results indicated that higher power treatment (≥540 W) significantly destabilized POBE. The optimal condition at 720 W increased POBE extraction yield and demulsification rate by 16.82% and 46.32%, respectively, compared with the control. This destabilization was attributed to marked changes in interfacial properties, including decreased apparent viscosity, lowered absolute ζ-potential (from 35.93 mV to 27.09 mV), increased particle size (from 1177.16 nm to 1976.98 nm), and the microstructure of droplet aggregation. Compositional analysis revealed that microwave treatment induced POBE reorganization, characterized by increased lipid and phospholipid contents alongside reduced moisture, solid, and protein levels. Further interfacial protein analysis revealed that exposure triggered protein conformational unfolding, hydrophobic group exposure, and subsequent aggregation, which weakened protein adsorption at the interface and reduced the mechanical strength of the interfacial film. These findings elucidate the mechanism of microwave-induced emulsion instability, providing a theoretical basis for enhancing oil extraction efficiency through raw material pretreatment.</p>","PeriodicalId":12386,"journal":{"name":"Foods","volume":"15 5","pages":""},"PeriodicalIF":5.1,"publicationDate":"2026-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12984753/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147456488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study was conducted to investigate and identify correlations among sensory and comprehensive consumer test results with rheological, textural, and tribological properties of milk chocolate in response to varying levels of particle size and emulsifier. To simulate realistic oral conditions, artificial saliva was incorporated into instrumental analyses. Rheological analysis revealed that increasing particle size and emulsifier concentration significantly reduced plastic viscosity, while emulsifier concentration alone increased yield stress due to structural reorganization within the fat phase. Tribological measurements demonstrated that larger particles increased friction in boundary and mixed lubrication regimes, whereas emulsifiers reduced friction in these regimes by enhancing fluid film formation. Under elastohydrodynamic conditions and with artificial saliva, friction was more influenced by the interaction between particle size and emulsifier level. Textural analysis showed that both parameters significantly influenced hardness, with saliva further softening the samples, especially those with higher emulsifier levels. Sensory evaluations indicated that emulsifiers enhanced flavor release and mouthfeel attributes, while smaller particles contributed to smoother texture and more balanced flavor perception. Consumer acceptance tests confirmed that samples with smaller particles and higher emulsifier levels received the highest scores in overall liking, taste, and texture. Instrumental parameters strongly correlated with key sensory attributes, with yield stress showing the highest positive associations with creaminess, smoothness, fat/milk flavor, and liking, while higher viscosity and friction were negatively linked to flavor release and mouthfeel. Instrumental hardness negatively correlated with cacao intensity and astringency, while saliva-induced softening was positively associated with sweetness and liking, highlighting the role of dynamic oral softening.
{"title":"Correlations Between Sensory Evaluations and Instrumental Measurements in Milk Chocolate with Varying Emulsifier Levels and Particle Sizes.","authors":"Burcu Sasmaz, Gurbuz Gunes","doi":"10.3390/foods15050938","DOIUrl":"10.3390/foods15050938","url":null,"abstract":"<p><p>This study was conducted to investigate and identify correlations among sensory and comprehensive consumer test results with rheological, textural, and tribological properties of milk chocolate in response to varying levels of particle size and emulsifier. To simulate realistic oral conditions, artificial saliva was incorporated into instrumental analyses. Rheological analysis revealed that increasing particle size and emulsifier concentration significantly reduced plastic viscosity, while emulsifier concentration alone increased yield stress due to structural reorganization within the fat phase. Tribological measurements demonstrated that larger particles increased friction in boundary and mixed lubrication regimes, whereas emulsifiers reduced friction in these regimes by enhancing fluid film formation. Under elastohydrodynamic conditions and with artificial saliva, friction was more influenced by the interaction between particle size and emulsifier level. Textural analysis showed that both parameters significantly influenced hardness, with saliva further softening the samples, especially those with higher emulsifier levels. Sensory evaluations indicated that emulsifiers enhanced flavor release and mouthfeel attributes, while smaller particles contributed to smoother texture and more balanced flavor perception. Consumer acceptance tests confirmed that samples with smaller particles and higher emulsifier levels received the highest scores in overall liking, taste, and texture. Instrumental parameters strongly correlated with key sensory attributes, with yield stress showing the highest positive associations with creaminess, smoothness, fat/milk flavor, and liking, while higher viscosity and friction were negatively linked to flavor release and mouthfeel. Instrumental hardness negatively correlated with cacao intensity and astringency, while saliva-induced softening was positively associated with sweetness and liking, highlighting the role of dynamic oral softening.</p>","PeriodicalId":12386,"journal":{"name":"Foods","volume":"15 5","pages":""},"PeriodicalIF":5.1,"publicationDate":"2026-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12984600/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147456608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Athanasia P Marangeli, Vassilios K Karabagias, Glykeria E Angelaki, Dimitrios G Lazaridis, Nikolaos D Andritsos, Olga Malisova, Ioannis K Karabagias
We studied the shelf life of fresh buffalo meat in polyamide/polyethylene (PA/PE) packaging during refrigerated storage for 14 days, when treated with cinnamon-clove (C-C) and nutmeg (Nut) powders, along with lavender essential oil (LEO). Microbiological (total viable count, Pseudomonas spp., Brochothrix thermosphacta, Enterobacteriaceae, and lactic acid bacteria), antibacterial (Salmonella Typhimurium and Staphylococcus aureus), physicochemical and biochemical (pH, moisture, color, total fat, hemoglobin and heme iron, 2-thiobarbituric acid, mercaptans, antioxidant activity, and total phenolic content), and sensory (color, odor, texture, and taste) analyses were carried out. The results showed that C-C and Nut powder extracts exhibited significant (p < 0.05) antioxidant and antibacterial activity, higher than LEO; however, all treatments delayed lipid oxidation. Based primarily on sensory evaluation, the shelf life extension of buffalo meat was 2-3 days for LEO and Nut powder, and 4-6 days for C-C powder. Factor analysis indicated the critical days of refrigerated storage for the evolution of spoilage-related biochemical parameters.
{"title":"Shelf Life Extension of Fresh Buffalo Meat Using Spice Powders and Lavender Essential Oil During Storage Under Refrigeration.","authors":"Athanasia P Marangeli, Vassilios K Karabagias, Glykeria E Angelaki, Dimitrios G Lazaridis, Nikolaos D Andritsos, Olga Malisova, Ioannis K Karabagias","doi":"10.3390/foods15050947","DOIUrl":"10.3390/foods15050947","url":null,"abstract":"<p><p>We studied the shelf life of fresh buffalo meat in polyamide/polyethylene (PA/PE) packaging during refrigerated storage for 14 days, when treated with cinnamon-clove (C-C) and nutmeg (Nut) powders, along with lavender essential oil (LEO). Microbiological (total viable count, <i>Pseudomonas</i> spp., <i>Brochothrix thermosphacta</i>, <i>Enterobacteriaceae</i>, and lactic acid bacteria), antibacterial (<i>Salmonella</i> Typhimurium and <i>Staphylococcus aureus</i>), physicochemical and biochemical (pH, moisture, color, total fat, hemoglobin and heme iron, 2-thiobarbituric acid, mercaptans, antioxidant activity, and total phenolic content), and sensory (color, odor, texture, and taste) analyses were carried out. The results showed that C-C and Nut powder extracts exhibited significant (<i>p</i> < 0.05) antioxidant and antibacterial activity, higher than LEO; however, all treatments delayed lipid oxidation. Based primarily on sensory evaluation, the shelf life extension of buffalo meat was 2-3 days for LEO and Nut powder, and 4-6 days for C-C powder. Factor analysis indicated the critical days of refrigerated storage for the evolution of spoilage-related biochemical parameters.</p>","PeriodicalId":12386,"journal":{"name":"Foods","volume":"15 5","pages":""},"PeriodicalIF":5.1,"publicationDate":"2026-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12984360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147456651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Persimmon polyphenols (PP) are natural polyphenols with high reactivity and strong deodorization potential; however, their practical application in odor control is limited by their poor solubility. In this study, natural deep eutectic solvents (NADESs) were employed for the green extraction of PP, and the capabilities of extracts on the removal of ammonia (NH3) and hydrogen sulfide (H2S) were investigated. In addition, the underlying mechanisms were explored by integrating spectroscopic analysis, molecular dynamics simulations, and quantum chemical calculations. The results showed that chloride-citric acid (CC-CA) was the optimal system in both PP extraction and sustained NH3 removal, while the betaine-urea (B-U) system was more effective for H2S removal. NH3 removal was governed by acid-base neutralization, with the resulting ammonium species being further stabilized within the PP-regulated NADES hydrogen-bond network. In contrast, H2S interacted with the solvent network not only through acid-base neutralization but also via Van der Waals forces and hydrophobic contacts. Our data supported that NADESs enhanced the deodorization performance of PP through cooperative microenvironment regulation rather than irreversible chemical conversion. This work highlighted that NADESs could not only function as highly efficient extraction media for polyphenols, but also active platforms for enhancing selective gas-capture capability for polyphenols. Furthermore, it provided a new strategy for the rational design of green, persimmon-derived deodorants.
{"title":"Mechanistic Insights into the Cooperative Removal of NH3 and H2S by Persimmon Polyphenols with Natural Deep Eutectic Solvent Systems","authors":"Baixue Li, Lu Li, Qingyun Guan, Chunmei Li","doi":"10.3390/foods15050939","DOIUrl":"https://doi.org/10.3390/foods15050939","url":null,"abstract":"Persimmon polyphenols (PP) are natural polyphenols with high reactivity and strong deodorization potential; however, their practical application in odor control is limited by their poor solubility. In this study, natural deep eutectic solvents (NADESs) were employed for the green extraction of PP, and the capabilities of extracts on the removal of ammonia (NH3) and hydrogen sulfide (H2S) were investigated. In addition, the underlying mechanisms were explored by integrating spectroscopic analysis, molecular dynamics simulations, and quantum chemical calculations. The results showed that chloride-citric acid (CC-CA) was the optimal system in both PP extraction and sustained NH3 removal, while the betaine-urea (B-U) system was more effective for H2S removal. NH3 removal was governed by acid-base neutralization, with the resulting ammonium species being further stabilized within the PP-regulated NADES hydrogen-bond network. In contrast, H2S interacted with the solvent network not only through acid-base neutralization but also via Van der Waals forces and hydrophobic contacts. Our data supported that NADESs enhanced the deodorization performance of PP through cooperative microenvironment regulation rather than irreversible chemical conversion. This work highlighted that NADESs could not only function as highly efficient extraction media for polyphenols, but also active platforms for enhancing selective gas-capture capability for polyphenols. Furthermore, it provided a new strategy for the rational design of green, persimmon-derived deodorants.","PeriodicalId":12386,"journal":{"name":"Foods","volume":"15 5","pages":"939-939"},"PeriodicalIF":0.0,"publicationDate":"2026-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147381691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
(1) Background: Babassu (Attalea speciosa) mesocarp is a functional food rich in nutrients and phenolic compounds, offering antioxidant and metabolic benefits. However, its effects on lipid peroxidation and pro-oxidant enzymes remain poorly explored. (2) Methods: The antioxidant potential of a hydroalcoholic extract of babassu mesocarp (HEB) was assessed using DPPH radical scavenging and lipid peroxidation inhibition, measured by the TBARS assay. Cytotoxicity was assessed by the MTT assay. Molecular docking was conducted to investigate interactions between HEB-derived compounds and NADPH oxidase and xanthine oxidase. (3) Results: HEB showed dose-dependent antioxidant activity (IC50 = 4.734 µg/mL) and effectively inhibited lipid peroxidation (IC50 = 51.35 µg/mL), with no cytotoxic effects. In silico analyses suggested potential inhibition of pro-oxidant enzymes. (4) Conclusions: HEB exhibits a strong ability to inhibit lipid peroxidation and theoretical enzyme-inhibitory potential, supporting its use in functional foods and nutraceuticals.
{"title":"Babassu (<i>Attalea speciosa</i>) Mesocarp Flour Extract Inhibits Lipid Peroxidation and Pro-Oxidant Enzymes: In Vitro and In Silico Evidence.","authors":"Caroline Chavier Pereira Santana, Fernanda Farias Costa, Jaqueline Daniele Santos Barros, Michelli Erica Souza Ferreira, Richard Pereira Dutra, Antônio Silva Machado, Aramys Silva Reis","doi":"10.3390/foods15050945","DOIUrl":"10.3390/foods15050945","url":null,"abstract":"<p><p>(1) Background: Babassu (<i>Attalea speciosa)</i> mesocarp is a functional food rich in nutrients and phenolic compounds, offering antioxidant and metabolic benefits. However, its effects on lipid peroxidation and pro-oxidant enzymes remain poorly explored. (2) Methods: The antioxidant potential of a hydroalcoholic extract of babassu mesocarp (HEB) was assessed using DPPH radical scavenging and lipid peroxidation inhibition, measured by the TBARS assay. Cytotoxicity was assessed by the MTT assay. Molecular docking was conducted to investigate interactions between HEB-derived compounds and NADPH oxidase and xanthine oxidase. (3) Results: HEB showed dose-dependent antioxidant activity (IC<sub>50</sub> = 4.734 µg/mL) and effectively inhibited lipid peroxidation (IC<sub>50</sub> = 51.35 µg/mL), with no cytotoxic effects. In silico analyses suggested potential inhibition of pro-oxidant enzymes. (4) Conclusions: HEB exhibits a strong ability to inhibit lipid peroxidation and theoretical enzyme-inhibitory potential, supporting its use in functional foods and nutraceuticals.</p>","PeriodicalId":12386,"journal":{"name":"Foods","volume":"15 5","pages":""},"PeriodicalIF":5.1,"publicationDate":"2026-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12984551/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147456569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Conventional immunochromatographic assays (ICAs) face limitations in sensitivity and dynamic range, hindering their application in on-site, class-specific screening of sulfonylurea (SU) adulteration in functional foods. To address this, a signal amplification strategy was developed by engineering high-density platinum nanozymes on a mesoporous metal-organic framework (PCN-224). The mesoporous architecture of PCN-224 facilitated high-density and stable loading of catalytically active Pt sites. The established PCN-224@Pt-based ICA achieved detection limits of 0.52-7.94 μg/kg in tea and 0.69-7.02 μg/kg in capsules, with linear ranges of 1.69-513.01 μg/kg and 2.05-716.47 μg/kg, respectively. Compared with traditional colloidal gold immunochromatographic assays (CG-ICAs), sensitivity was improved by up to 57-fold, while the linear detection range was expanded by over 5-fold relative to the previously reported PCN-224@PDA- ICA. The method demonstrated recovery rates of 81.8-119.8% and coefficients of variation between 2.5% and 11.4%. Validation against LC-MS/MS using 20 real samples showed excellent agreement (R2 > 0.99). This work not only provides a sensitive and rapid tool for the surveillance of SU adulteration in functional foods but also establishes a generalizable nanozyme design strategy applicable to enhancing the performance of a wide range of ICA-based detection platforms.
{"title":"Class-Specific Immunochromatographic Assay Enabled by Mesoporous Nanozyme-Catalyzed Signal Amplification for On-Site Screening of Sulfonylureas.","authors":"Yanting Li, Zixian He, Pengjie He, Zixuan Tang, Esra Bağda, Efkan Bağda, Zhenlin Xu, Xiangmei Li","doi":"10.3390/foods15050944","DOIUrl":"10.3390/foods15050944","url":null,"abstract":"<p><p>Conventional immunochromatographic assays (ICAs) face limitations in sensitivity and dynamic range, hindering their application in on-site, class-specific screening of sulfonylurea (SU) adulteration in functional foods. To address this, a signal amplification strategy was developed by engineering high-density platinum nanozymes on a mesoporous metal-organic framework (PCN-224). The mesoporous architecture of PCN-224 facilitated high-density and stable loading of catalytically active Pt sites. The established PCN-224@Pt-based ICA achieved detection limits of 0.52-7.94 μg/kg in tea and 0.69-7.02 μg/kg in capsules, with linear ranges of 1.69-513.01 μg/kg and 2.05-716.47 μg/kg, respectively. Compared with traditional colloidal gold immunochromatographic assays (CG-ICAs), sensitivity was improved by up to 57-fold, while the linear detection range was expanded by over 5-fold relative to the previously reported PCN-224@PDA- ICA. The method demonstrated recovery rates of 81.8-119.8% and coefficients of variation between 2.5% and 11.4%. Validation against LC-MS/MS using 20 real samples showed excellent agreement (R<sup>2</sup> > 0.99). This work not only provides a sensitive and rapid tool for the surveillance of SU adulteration in functional foods but also establishes a generalizable nanozyme design strategy applicable to enhancing the performance of a wide range of ICA-based detection platforms.</p>","PeriodicalId":12386,"journal":{"name":"Foods","volume":"15 5","pages":""},"PeriodicalIF":5.1,"publicationDate":"2026-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12984563/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147456537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Salt reduction is an important strategy for healthy diets. Our previous study developed low-sodium chickpea nang (LCHN) using potassium chloride, wheat gluten, inulin and L-lysine. However, consumers also value taste. The impact of this reformulation on oral processing characteristics remains unclear. This study collected chewing samples from 12 volunteers at five mastication stages (0%, 25%, 50%, 75%, and 100%) of regular chickpea nang (CHN) and LCHN, measuring chewing parameters, bolus moisture content, saliva addition amount, and flow rate. Results indicated that LCHN had a significantly shorter swallowing time (24.22 ± 3.63 s vs. 27.84 ± 6.01 s, p < 0.05, Cohen's d = 0.73), while the number of chews (Nc), chewing frequency (Fc), bolus moisture content, and saliva flow rate showed no inter-group differences across all mastication stages (p > 0.05). Bolus moisture content increased significantly with mastication progression in both groups (p < 0.05), whereas saliva addition amount and flow rate decreased significantly (p < 0.05). Additionally, higher chewing frequency correlated with increased saliva addition amount and reduced flow rate (p < 0.05). In CHN, the Nc positively correlated with chewing time (r = 0.452, p < 0.01) and frequency (r = 0.458, p < 0.01), whereas in LCHN it negatively correlated with time (r = -0.329, p < 0.05) and positively with frequency (r = 0.884, p < 0.01). These findings provide theoretical basis for low-sodium baked product development.
减少食盐是健康饮食的一项重要策略。本研究利用氯化钾、小麦面筋、菊粉和l -赖氨酸制备了低钠鹰嘴豆粕。然而,消费者也重视口味。这种重新配方对口腔加工特征的影响尚不清楚。本研究采集了12名志愿者在普通鹰嘴豆(CHN)和LCHN五个咀嚼阶段(0%、25%、50%、75%和100%)的咀嚼样本,测量了咀嚼参数、丸含水量、唾液添加量和流速。结果显示,LCHN大鼠吞咽时间(24.22±3.63 s vs. 27.84±6.01 s, p < 0.05, Cohen’s d = 0.73)明显短于LCHN大鼠(24.22±3.63 s vs. 27.84±6.01 s),各组咀嚼次数(Nc)、咀嚼频率(Fc)、颗粒水分含量、唾液流率在各咀嚼阶段无组间差异(p < 0.05)。随着咀嚼的进行,两组动物的颗粒水分含量均显著增加(p < 0.05),唾液添加量和流速均显著降低(p < 0.05)。咀嚼频率越高,唾液添加量越高,唾液流率越低(p < 0.05)。CHN与咀嚼时间(r = 0.452, p < 0.01)、咀嚼频率(r = 0.458, p < 0.01)呈正相关,LCHN与咀嚼时间(r = -0.329, p < 0.05)负相关,与咀嚼频率(r = 0.884, p < 0.01)正相关。研究结果为低钠烘焙产品的开发提供了理论依据。
{"title":"Effects of Salt-Reducing Alternatives on the Oral Processing Characteristics of Chickpea Nang.","authors":"Qian Wang, Ying Li, Sailimuhan Asimi","doi":"10.3390/foods15050941","DOIUrl":"10.3390/foods15050941","url":null,"abstract":"<p><p>Salt reduction is an important strategy for healthy diets. Our previous study developed low-sodium chickpea nang (LCHN) using potassium chloride, wheat gluten, inulin and L-lysine. However, consumers also value taste. The impact of this reformulation on oral processing characteristics remains unclear. This study collected chewing samples from 12 volunteers at five mastication stages (0%, 25%, 50%, 75%, and 100%) of regular chickpea nang (CHN) and LCHN, measuring chewing parameters, bolus moisture content, saliva addition amount, and flow rate. Results indicated that LCHN had a significantly shorter swallowing time (24.22 ± 3.63 s vs. 27.84 ± 6.01 s, <i>p</i> < 0.05, Cohen's d = 0.73), while the number of chews (Nc), chewing frequency (Fc), bolus moisture content, and saliva flow rate showed no inter-group differences across all mastication stages (<i>p</i> > 0.05). Bolus moisture content increased significantly with mastication progression in both groups (<i>p</i> < 0.05), whereas saliva addition amount and flow rate decreased significantly (<i>p</i> < 0.05). Additionally, higher chewing frequency correlated with increased saliva addition amount and reduced flow rate (<i>p</i> < 0.05). In CHN, the Nc positively correlated with chewing time (r = 0.452, <i>p</i> < 0.01) and frequency (r = 0.458, <i>p</i> < 0.01), whereas in LCHN it negatively correlated with time (r = -0.329, <i>p</i> < 0.05) and positively with frequency (r = 0.884, <i>p</i> < 0.01). These findings provide theoretical basis for low-sodium baked product development.</p>","PeriodicalId":12386,"journal":{"name":"Foods","volume":"15 5","pages":""},"PeriodicalIF":5.1,"publicationDate":"2026-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12985187/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147456699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}