Cite this article: Roy A, Ghosh S, Chakraborty R. Comparative Research of Anti-Inflammatory Effects of Different Dietary Antioxidants on Alcohol-Induced Damage in Gastric Cells. Free Radicals and Antioxidants. 2019;9(1):16-21. ABSTRACT Objective: Antioxidants prevent ill-effects of free radicals through regulation of different proand anti-inflammatory enzymes of the cell which have association with the pathophysiology of alcohol-induced gastropathy. This study was aimed to explore the mechanism in gastric adenocarcinoma (AGS) cell line and protection by dietary antioxidants e.g. catechin, resveratrol, quercetin, curcumin and 6-gingerol. Methods: AGS cells were exposed to 2.5% ethanol for varied time span in presence and absence of antioxidants. Cytotoxicity was assessed by MTT assay and annexin V-propidium iodide staining. The damages in cellular morphology were observed by DAPI and EtBr-acridine orange staining. Changes in ROS generation were examined through confocal microscopy. Western blots were performed using cell extracts to investigate the changes in expression level of COX-1, -2, catalase, superoxide dismutase (SOD), iNOS, MMP-9 and TIMP-1. Immunofluorescence study was done to cross check the expression of COX-2. Results: A concentration-dependent cytotoxicity was observed in the cell viability on ethanol exposure which was reduced by antioxidant treatment. Antioxidants reduced the inflammation by downregulating the expression of COX-2, iNOS and MMP-9 and by upregulating the expressions of catalase, SOD and TIMP-1 significantly. Catechin and quercetin demonstrated most prominent cytoprotection amongst the five, followed by resveratrol. These results were corroborated with MTT assay, DAPI and EtBr-acridine orange staining. Curcumin and 6-gingerol did not show any significant effect. Conclusion: Dietary antioxidants protect AGS cells from oxidative stress by maintaining the homeostasis between oxidant-antioxidant and protease-antiprotease ratio.
{"title":"Comparative Research on Anti-Inflammatory Effects of Different Dietary Antioxidants on Alcohol-Induced Damage in Gastric Cells","authors":"A. Roy, Satyabrata Ghosh, R. Chakraborty","doi":"10.5530/FRA.2019.1.4","DOIUrl":"https://doi.org/10.5530/FRA.2019.1.4","url":null,"abstract":"Cite this article: Roy A, Ghosh S, Chakraborty R. Comparative Research of Anti-Inflammatory Effects of Different Dietary Antioxidants on Alcohol-Induced Damage in Gastric Cells. Free Radicals and Antioxidants. 2019;9(1):16-21. ABSTRACT Objective: Antioxidants prevent ill-effects of free radicals through regulation of different proand anti-inflammatory enzymes of the cell which have association with the pathophysiology of alcohol-induced gastropathy. This study was aimed to explore the mechanism in gastric adenocarcinoma (AGS) cell line and protection by dietary antioxidants e.g. catechin, resveratrol, quercetin, curcumin and 6-gingerol. Methods: AGS cells were exposed to 2.5% ethanol for varied time span in presence and absence of antioxidants. Cytotoxicity was assessed by MTT assay and annexin V-propidium iodide staining. The damages in cellular morphology were observed by DAPI and EtBr-acridine orange staining. Changes in ROS generation were examined through confocal microscopy. Western blots were performed using cell extracts to investigate the changes in expression level of COX-1, -2, catalase, superoxide dismutase (SOD), iNOS, MMP-9 and TIMP-1. Immunofluorescence study was done to cross check the expression of COX-2. Results: A concentration-dependent cytotoxicity was observed in the cell viability on ethanol exposure which was reduced by antioxidant treatment. Antioxidants reduced the inflammation by downregulating the expression of COX-2, iNOS and MMP-9 and by upregulating the expressions of catalase, SOD and TIMP-1 significantly. Catechin and quercetin demonstrated most prominent cytoprotection amongst the five, followed by resveratrol. These results were corroborated with MTT assay, DAPI and EtBr-acridine orange staining. Curcumin and 6-gingerol did not show any significant effect. Conclusion: Dietary antioxidants protect AGS cells from oxidative stress by maintaining the homeostasis between oxidant-antioxidant and protease-antiprotease ratio.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81219894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cite this article: Wali AF, Alam A. The Effect of Different Extraction Methods on Antioxidant Capacity and Phytochemical Screening of Syzygium cumini Seeds. Free Radicals and Antioxidants. 2019;9(1):48-51. ABSTRACT Objective: Syzygium cumini has been used for centuries in traditional medicine for various athological conditions. In the present study, extract yield, phytochemical constituents and in-vitro antioxidant assay ethanolic extracts of Syzygium cumini seeds was investigated. Methods: Extraction techniques like ultrasound microwave assisted solvent, maceration and soxhlet were used for extraction. The extracts were evaluated for potential antioxidant activities by using DPPH and ABTS assay. Results: Our results revealed that extract yield, chemical composition of the extracts and antioxidant activity of the Syzygium cumini extract varied with the extraction process. The results exhibited highest extraction yield and flavonoids, alkaloids and glycoside in ultrasound microwave assisted solvent extraction followed by soxhlet extraction and least in maceration extraction method respectively. All extraction methods showed free radical scavenging potential, ultrasound microwave assisted solvent extraction exhibited significant scavenging potential having an IC50 value of 69 ± 8.11 μg/mL for DPPH and 87 ± 5.86 μg/mL for ABTS. Naringin is used as a reference standard antioxidant agent. Conclusion: These results revealed that the ultrasound microwave assisted solvent extraction of Syzygium cumini seeds can be a rich source of antioxidants containing flavonoids, alkaloids and glycoside. The antioxidants chemical compounds present in Syzygium cumini seeds have various beneficial effects in the phytopharmaceuticals industry.
{"title":"The Effect of Different Extraction Methods on Antioxidant Capacity and Phytochemical Screening of Syzygium cumini Seeds","authors":"A. Wali, A. Alam","doi":"10.5530/FRA.2019.1.9","DOIUrl":"https://doi.org/10.5530/FRA.2019.1.9","url":null,"abstract":"Cite this article: Wali AF, Alam A. The Effect of Different Extraction Methods on Antioxidant Capacity and Phytochemical Screening of Syzygium cumini Seeds. Free Radicals and Antioxidants. 2019;9(1):48-51. ABSTRACT Objective: Syzygium cumini has been used for centuries in traditional medicine for various athological conditions. In the present study, extract yield, phytochemical constituents and in-vitro antioxidant assay ethanolic extracts of Syzygium cumini seeds was investigated. Methods: Extraction techniques like ultrasound microwave assisted solvent, maceration and soxhlet were used for extraction. The extracts were evaluated for potential antioxidant activities by using DPPH and ABTS assay. Results: Our results revealed that extract yield, chemical composition of the extracts and antioxidant activity of the Syzygium cumini extract varied with the extraction process. The results exhibited highest extraction yield and flavonoids, alkaloids and glycoside in ultrasound microwave assisted solvent extraction followed by soxhlet extraction and least in maceration extraction method respectively. All extraction methods showed free radical scavenging potential, ultrasound microwave assisted solvent extraction exhibited significant scavenging potential having an IC50 value of 69 ± 8.11 μg/mL for DPPH and 87 ± 5.86 μg/mL for ABTS. Naringin is used as a reference standard antioxidant agent. Conclusion: These results revealed that the ultrasound microwave assisted solvent extraction of Syzygium cumini seeds can be a rich source of antioxidants containing flavonoids, alkaloids and glycoside. The antioxidants chemical compounds present in Syzygium cumini seeds have various beneficial effects in the phytopharmaceuticals industry.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"525 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79544432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cite this article: Shastri AA, Spallholz JE. Catalytic Generation of Superoxide by Different Alcohols. Free Radicals and Antioxidants. 2019;9(1):43-7. ABSTRACT Objective: Group VIA elements, oxygen, sulfur and selenium can be toxic and many of their compounds are toxic to cells owing to the catalytic generation of superoxide and oxidative stress from thiol oxidations. Sulfides, (RS-) and selenides, (RSe-) of organic molecules and enzymes are often redox catalysts. In the current study, alcohols (ROH) were investigated to ascertain if oxides (RO-) of some alcohols might also ionize and redox cycle generating superoxide. Methods: The Lucigenin chemiluminescence assay was used for the detection of superoxide generation by the aliphatic alcohols and Benzyl Alcohol at 25°C and 37°C in the presence or absence of reduced glutathione (GSH). Similar Benzyl compounds of sulfur, selenium and oxygen were also tested for direct comparison of their catalytic activity. Results: Many of the alcohols tested, generated superoxide in the presence of GSH at both 25°C and 37°C, but not in the absence of GSH. Overall catalytic activity was greater at 37°C than at 25°C. Comparing the catalytic activity of equal concentrations of the S, Se and O moiety of the Benzyl compounds showed that although the catalytic alcohols did generate superoxide in the presence of GSH, but the sulfur and selenium compounds showed greater catalytic activity. Conclusion: As hypothesized, some aliphatic alcohols tested did generate superoxide similar to many sulfur and selenium analog compounds in the presence of GSH. From the results we can deduce that some alcohols may be following a redox mechanism that is similar to the S and Se compounds that redox cycle in presence of GSH generating superoxide.
{"title":"Catalytic Generation of Superoxide by Different Alcohols","authors":"Anuradha Shastri, J. Spallholz","doi":"10.5530/FRA.2019.1.8","DOIUrl":"https://doi.org/10.5530/FRA.2019.1.8","url":null,"abstract":"Cite this article: Shastri AA, Spallholz JE. Catalytic Generation of Superoxide by Different Alcohols. Free Radicals and Antioxidants. 2019;9(1):43-7. ABSTRACT Objective: Group VIA elements, oxygen, sulfur and selenium can be toxic and many of their compounds are toxic to cells owing to the catalytic generation of superoxide and oxidative stress from thiol oxidations. Sulfides, (RS-) and selenides, (RSe-) of organic molecules and enzymes are often redox catalysts. In the current study, alcohols (ROH) were investigated to ascertain if oxides (RO-) of some alcohols might also ionize and redox cycle generating superoxide. Methods: The Lucigenin chemiluminescence assay was used for the detection of superoxide generation by the aliphatic alcohols and Benzyl Alcohol at 25°C and 37°C in the presence or absence of reduced glutathione (GSH). Similar Benzyl compounds of sulfur, selenium and oxygen were also tested for direct comparison of their catalytic activity. Results: Many of the alcohols tested, generated superoxide in the presence of GSH at both 25°C and 37°C, but not in the absence of GSH. Overall catalytic activity was greater at 37°C than at 25°C. Comparing the catalytic activity of equal concentrations of the S, Se and O moiety of the Benzyl compounds showed that although the catalytic alcohols did generate superoxide in the presence of GSH, but the sulfur and selenium compounds showed greater catalytic activity. Conclusion: As hypothesized, some aliphatic alcohols tested did generate superoxide similar to many sulfur and selenium analog compounds in the presence of GSH. From the results we can deduce that some alcohols may be following a redox mechanism that is similar to the S and Se compounds that redox cycle in presence of GSH generating superoxide.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79709343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cite this article: Nawaz H, Aslam M, Muntaha ST. Effect of Solvent Polarity and Extraction Method on Phytochemical Composition and Antioxidant Potential of Corn Silk. Free Radicals and Antioxidants. 2019;9(1):5-11. ABSTRACT Objectives: Corn silk is an underutilized part of corn which possesses great medicinal importance. The present study was planned to determine the phytochemical composition and antioxidant potential of corn silk extracts, obtained by different extraction methods using a series of solvents with increasing polarity. Methods: Three extraction methods 1) individual extraction in each solvent, 2) consecutive extraction in solvents of increasing polarity and 3) consecutive extraction of crude methanolic extract in solvents of increasing polarity and a series of five solvents with increasing polarity were used for extraction of phytochemicals. The extracts were analyzed for phytochemical composition and antioxidant potential. Results: Corn silk was found to contain a variety of bioactive phytochemical compounds including phenolic acids, flavonoids, ascorbic acid, tannins and cardiac glycosides. The corn silk phytochemicals were extracted more in high polarity solvents which showed comparatively good phytochemical composition and strong antioxidants potential. Regression analysis of experimental data showed a polarity dependent increase in extraction yield and phytochemical content and free radical scavenging capacity of extracts obtained by various extraction methods. Conclusion: The water extract obtained by individual extraction showed a comparatively high extraction yield and phytochemical content while that obtained by consecutive extraction of crude methanolic extract showed high ability to scavenge free radicals. The study advocates the corn silk as a good source of antioxidant phytochemicals and suggests the use of polar solvents and individual extraction method for extraction of corn silk phytochemicals.
{"title":"Effect of Solvent Polarity and Extraction Method on Phytochemical Composition and Antioxidant Potential of Corn Silk","authors":"H. Nawaz, M. Aslam, S. Muntaha","doi":"10.5530/FRA.2019.1.2","DOIUrl":"https://doi.org/10.5530/FRA.2019.1.2","url":null,"abstract":"Cite this article: Nawaz H, Aslam M, Muntaha ST. Effect of Solvent Polarity and Extraction Method on Phytochemical Composition and Antioxidant Potential of Corn Silk. Free Radicals and Antioxidants. 2019;9(1):5-11. ABSTRACT Objectives: Corn silk is an underutilized part of corn which possesses great medicinal importance. The present study was planned to determine the phytochemical composition and antioxidant potential of corn silk extracts, obtained by different extraction methods using a series of solvents with increasing polarity. Methods: Three extraction methods 1) individual extraction in each solvent, 2) consecutive extraction in solvents of increasing polarity and 3) consecutive extraction of crude methanolic extract in solvents of increasing polarity and a series of five solvents with increasing polarity were used for extraction of phytochemicals. The extracts were analyzed for phytochemical composition and antioxidant potential. Results: Corn silk was found to contain a variety of bioactive phytochemical compounds including phenolic acids, flavonoids, ascorbic acid, tannins and cardiac glycosides. The corn silk phytochemicals were extracted more in high polarity solvents which showed comparatively good phytochemical composition and strong antioxidants potential. Regression analysis of experimental data showed a polarity dependent increase in extraction yield and phytochemical content and free radical scavenging capacity of extracts obtained by various extraction methods. Conclusion: The water extract obtained by individual extraction showed a comparatively high extraction yield and phytochemical content while that obtained by consecutive extraction of crude methanolic extract showed high ability to scavenge free radicals. The study advocates the corn silk as a good source of antioxidant phytochemicals and suggests the use of polar solvents and individual extraction method for extraction of corn silk phytochemicals.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76422901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Khalida F. Al-azawi, Dunya L. AL-Duhaidahawi, A. Al-Amiery, A. Kadhum
Cite this article: Al-Azawi K, AL-Duhaidahawi DL, Al-Amiery A, Kadhum AAH. Synthesis of Schiff base Metal Complexes with Motivating Scavenging Potential Studies. Free Radicals and Antioxidants. 2019;9(1):1-4. ABSTRACT Objective: Schiff’s base complexes can be important substitutes for some antioxidant drugs because they have a carbon-nitrogen (N-C) double bond with the ability to push the electrons into the transition metal. Methods: A series of Schiff base metal complexes have been synthesized by the condensation of MCl2.nH2O (M = Co, Ni, Cu) with 1-(2hydroxybenzylideneamino) quinolin-2(1H)-one. Results: The Schiff base and it metal complexes have been characterized by various instrumental techniques like element chemical analysis, molar conductance, magnetic susceptibility measurements and spectral studies. The investigated complexes were subjected to antioxidant studies. The investigated complexes showed an excellent activities as antioxidant as compared to the parent Schiff base and standard compound. Conclusion: The data showed that transition metal complexes have significant improved antioxidant activity than Schiff base.
{"title":"Synthesis of Schiff base Metal Complexes with Motivating Scavenging Potential Studies","authors":"Khalida F. Al-azawi, Dunya L. AL-Duhaidahawi, A. Al-Amiery, A. Kadhum","doi":"10.5530/FRA.2019.1.1","DOIUrl":"https://doi.org/10.5530/FRA.2019.1.1","url":null,"abstract":"Cite this article: Al-Azawi K, AL-Duhaidahawi DL, Al-Amiery A, Kadhum AAH. Synthesis of Schiff base Metal Complexes with Motivating Scavenging Potential Studies. Free Radicals and Antioxidants. 2019;9(1):1-4. ABSTRACT Objective: Schiff’s base complexes can be important substitutes for some antioxidant drugs because they have a carbon-nitrogen (N-C) double bond with the ability to push the electrons into the transition metal. Methods: A series of Schiff base metal complexes have been synthesized by the condensation of MCl2.nH2O (M = Co, Ni, Cu) with 1-(2hydroxybenzylideneamino) quinolin-2(1H)-one. Results: The Schiff base and it metal complexes have been characterized by various instrumental techniques like element chemical analysis, molar conductance, magnetic susceptibility measurements and spectral studies. The investigated complexes were subjected to antioxidant studies. The investigated complexes showed an excellent activities as antioxidant as compared to the parent Schiff base and standard compound. Conclusion: The data showed that transition metal complexes have significant improved antioxidant activity than Schiff base.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80150361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cite this article: Kimondo J, Mutai P, Njogu P, Kimwele C. Evaluation of the Antioxidant Activity of Nine Plants Used Medicinally by the Ilkisonko Maasai Community of Kenya. Free Radicals and Antioxidants. 2019;9(1):29-34. ABSTRACT Objectives: Many plants used in ethnomedicinal interventions have anti-inflammatory, antibacterial, antiviral, anti-aging and anticancer activities attributed to their antioxidant properties. The antioxidant properties may be attributed to high polyphenol content of the plants. The Ilkisonko Maasai are a pastoralist community from Kenya known to ingest certain select plant decoctions for prevention or treatment of targeted illnesses. This study evaluated the antioxidant properties of organic and aqueous extracts of these select plants. Methods: The antioxidant potential was evaluated by 1, 1-diphenyl-2-picrylhydrazyl free radical (DPPH) scavenging method. Total phenolic and flavonoid content were determined using the Folin–Ciocalteu’s assay and aluminium chloride colorimetric test, respectively. Results: The organic extracts had significantly higher phenolic and flavonoid content than the aqueous extracts except for Pappea capensis in which the converse was observed. Among the studied plant extracts, Acacia nilotica had the highest phenolic content in the methanol and water extracts (237.26±1.83 mg and 149.66±0.60 mg tannic acid equivalent/g of extract) and the highest antioxidant activity in both the methanol and water extracts with an IC50 of 54.61μg/mL and 102.96μg/mL, respectively. The standard, ascorbic acid, had an IC50 of 50.32μg/mL. In both the methanol and water extracts, Acacia reficiens had the highest flavonoid content (130.62±1.78 and 99.80±1.73 mg catechin equivalents/g of extract, respectively). The correlation between the total phenolic content and antioxidant activity was statistically significant (Pearson’s r= -0.841). Conclusion: This study found that select plants used by the Ilkisonko Maasai as medicinal plants exhibited high phenolic content and antioxidant activity, giving credence to their ethnomedicinal use.
{"title":"Evaluation of the Antioxidant Activity of Nine Plants Used Medicinally by the Ilkisonko Maasai Community of Kenya","authors":"J. Kimondo, P. Mutai, P. Njogu, C. Kimwele","doi":"10.5530/FRA.2019.1.6","DOIUrl":"https://doi.org/10.5530/FRA.2019.1.6","url":null,"abstract":"Cite this article: Kimondo J, Mutai P, Njogu P, Kimwele C. Evaluation of the Antioxidant Activity of Nine Plants Used Medicinally by the Ilkisonko Maasai Community of Kenya. Free Radicals and Antioxidants. 2019;9(1):29-34. ABSTRACT Objectives: Many plants used in ethnomedicinal interventions have anti-inflammatory, antibacterial, antiviral, anti-aging and anticancer activities attributed to their antioxidant properties. The antioxidant properties may be attributed to high polyphenol content of the plants. The Ilkisonko Maasai are a pastoralist community from Kenya known to ingest certain select plant decoctions for prevention or treatment of targeted illnesses. This study evaluated the antioxidant properties of organic and aqueous extracts of these select plants. Methods: The antioxidant potential was evaluated by 1, 1-diphenyl-2-picrylhydrazyl free radical (DPPH) scavenging method. Total phenolic and flavonoid content were determined using the Folin–Ciocalteu’s assay and aluminium chloride colorimetric test, respectively. Results: The organic extracts had significantly higher phenolic and flavonoid content than the aqueous extracts except for Pappea capensis in which the converse was observed. Among the studied plant extracts, Acacia nilotica had the highest phenolic content in the methanol and water extracts (237.26±1.83 mg and 149.66±0.60 mg tannic acid equivalent/g of extract) and the highest antioxidant activity in both the methanol and water extracts with an IC50 of 54.61μg/mL and 102.96μg/mL, respectively. The standard, ascorbic acid, had an IC50 of 50.32μg/mL. In both the methanol and water extracts, Acacia reficiens had the highest flavonoid content (130.62±1.78 and 99.80±1.73 mg catechin equivalents/g of extract, respectively). The correlation between the total phenolic content and antioxidant activity was statistically significant (Pearson’s r= -0.841). Conclusion: This study found that select plants used by the Ilkisonko Maasai as medicinal plants exhibited high phenolic content and antioxidant activity, giving credence to their ethnomedicinal use.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"194 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79784157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hina Saeed, H. Nawaz, M. Shad, D. Shahwar, H. Andaleeb, S. Muzaffar, R. Jabeen, T. Rehman, A. Waheed
Cite this article: Saeed H, Nawaz H, Shad MA, Shahwar DE, Andaleeb H, Muzaffar S, et al. Antioxidant Potential of Cell Wall Polysaccharides Extracted from Various Parts of Aerva javanica. Free Radicals and Antioxidants. 2019;9(1):35-42. ABSTRACT Objectives: Aerva javanica, an underutilized desert plant, is a rich source of polysaccharide but not investigated properly for the antioxidant potential of its polysaccharides. The antioxidant potential of cell wall polysaccharides in leaves, branches, stem and root of A. javanica was determined. Methods: In a sequential extraction procedure, the water-soluble fraction (WSF), sodium acetate-EDTA soluble fraction (SASF), sodium carbonate soluble fraction (SCSF), 4% potassium hydroxide soluble fraction (4%KOHSF) and 14% potassium hydroxide soluble fraction (14%KOHSF) were obtained and subjected to antioxidant analysis. Results: The addition of salt or alkali in the extraction media resulted in a significant increase in total extractable polysaccharide content. The 14%KOHSF showed higher extract yield, total antioxidant activity, ferrous ion chelating activity, ferric reducing antioxidant power and linoleic acid reduction capacity. The WSF showed higher scavenging capacity against DPPH radical. WSF of branches and root and SCSF of leaf and stem showed highest scavenging capacity against hydroxyl radical. Conclusion: The lower extraction yield but high radical scavenging capacity of WSF may be attributed to the presence of some protein linked hydrophilic polysaccharides of pectin nature. The high extractable polysaccharide content with strong antioxidant potential makes A. javanica a suitable candidate for the antioxidant polysaccharide based pharmaceutical and industrial applications.
本文来源:Saeed H, Nawaz H, Shad MA, Shahwar DE, Andaleeb H, Muzaffar S，等。水芹不同部位细胞壁多糖的抗氧化活性研究。自由基与抗氧化剂[j] . 2019;9(1):35-42。摘要目的:javanica Aerva是一种未被充分利用的沙漠植物，是多糖的丰富来源，但其多糖的抗氧化潜力尚未得到适当的研究。对爪哇叶、枝、茎、根细胞壁多糖的抗氧化能力进行了测定。方法:采用顺序提取法，提取水溶部分(WSF)、乙酸钠- edta溶部分(SASF)、碳酸钠溶部分(SCSF)、4%氢氧化钾溶部分(4% kohsf)和14%氢氧化钾溶部分(14% kohsf)，并进行抗氧化分析。结果:在提取液中加入盐或碱均可显著提高总可提取多糖含量。14%KOHSF具有较高的提取率、总抗氧化活性、铁离子螯合活性、铁还原抗氧化能力和亚油酸还原能力。WSF对DPPH自由基具有较强的清除能力。对羟基自由基清除能力最强的是枝、根的WSF和叶、茎的SCSF。结论:枸杞多糖提取率低，清除自由基能力强，可能与其含有果胶性质的亲水蛋白多糖有关。爪哇多糖提取率高，具有较强的抗氧化潜力，是抗氧化多糖在制药和工业上的理想选择。
{"title":"Antioxidant Potential of Cell Wall Polysaccharides Extracted from Various Parts of Aerva javanica","authors":"Hina Saeed, H. Nawaz, M. Shad, D. Shahwar, H. Andaleeb, S. Muzaffar, R. Jabeen, T. Rehman, A. Waheed","doi":"10.5530/FRA.2019.1.7","DOIUrl":"https://doi.org/10.5530/FRA.2019.1.7","url":null,"abstract":"Cite this article: Saeed H, Nawaz H, Shad MA, Shahwar DE, Andaleeb H, Muzaffar S, et al. Antioxidant Potential of Cell Wall Polysaccharides Extracted from Various Parts of Aerva javanica. Free Radicals and Antioxidants. 2019;9(1):35-42. ABSTRACT Objectives: Aerva javanica, an underutilized desert plant, is a rich source of polysaccharide but not investigated properly for the antioxidant potential of its polysaccharides. The antioxidant potential of cell wall polysaccharides in leaves, branches, stem and root of A. javanica was determined. Methods: In a sequential extraction procedure, the water-soluble fraction (WSF), sodium acetate-EDTA soluble fraction (SASF), sodium carbonate soluble fraction (SCSF), 4% potassium hydroxide soluble fraction (4%KOHSF) and 14% potassium hydroxide soluble fraction (14%KOHSF) were obtained and subjected to antioxidant analysis. Results: The addition of salt or alkali in the extraction media resulted in a significant increase in total extractable polysaccharide content. The 14%KOHSF showed higher extract yield, total antioxidant activity, ferrous ion chelating activity, ferric reducing antioxidant power and linoleic acid reduction capacity. The WSF showed higher scavenging capacity against DPPH radical. WSF of branches and root and SCSF of leaf and stem showed highest scavenging capacity against hydroxyl radical. Conclusion: The lower extraction yield but high radical scavenging capacity of WSF may be attributed to the presence of some protein linked hydrophilic polysaccharides of pectin nature. The high extractable polysaccharide content with strong antioxidant potential makes A. javanica a suitable candidate for the antioxidant polysaccharide based pharmaceutical and industrial applications.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91300317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cite this article: Behera SK. Phytochemical Analysis and Antioxidant Activities of Gymnema sylvestre R. Br. Leaf Extracts. Free Radicals and Antioxidants. 2019;9(1):12-5. ABSTRACT Objective: The present study aims to determine the antioxidant properties and phytochemical screening of Gymnema sylvestre plant. Materials and Methods: The qualitative and quantitative analyses were carried out for the methanol extract using standard procedures. The antioxidant activities were carried out by DPPH free radical, OHradical, H2O2 radical, O2 radical and phosphomolybdenum reduction assay. Results: The leaves of G. sylvestre methanol extract showed good radical scavenging as well as reducing power activities. The total phenolic and flavonoid content were 19.87 ± 0.32 mg/g and 2.65 ± 0.12 mg/g respectively. Conclusion: The methanol extract of leaves of G. sylvestre possesses significant antioxidant activities.
{"title":"Phytochemical Analysis and Antioxidant Activities of Gymnema sylvestre R. Br. Leaf Extracts","authors":"Suraj K Behera","doi":"10.5530/FRA.2019.1.3","DOIUrl":"https://doi.org/10.5530/FRA.2019.1.3","url":null,"abstract":"Cite this article: Behera SK. Phytochemical Analysis and Antioxidant Activities of Gymnema sylvestre R. Br. Leaf Extracts. Free Radicals and Antioxidants. 2019;9(1):12-5. ABSTRACT Objective: The present study aims to determine the antioxidant properties and phytochemical screening of Gymnema sylvestre plant. Materials and Methods: The qualitative and quantitative analyses were carried out for the methanol extract using standard procedures. The antioxidant activities were carried out by DPPH free radical, OHradical, H2O2 radical, O2 radical and phosphomolybdenum reduction assay. Results: The leaves of G. sylvestre methanol extract showed good radical scavenging as well as reducing power activities. The total phenolic and flavonoid content were 19.87 ± 0.32 mg/g and 2.65 ± 0.12 mg/g respectively. Conclusion: The methanol extract of leaves of G. sylvestre possesses significant antioxidant activities.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87851691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation and Comparison of Antioxidant Activity of Leaves, Pericarps and Pulps of Three Garcinia Species in Malaysia Yik-Ling Chew, and Yau-Yan Lim","authors":"Yik-Ling Chew, Y. Lim","doi":"10.5530/FRA.2018.2.19","DOIUrl":"https://doi.org/10.5530/FRA.2018.2.19","url":null,"abstract":"","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"124 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88038779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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