Cite this article: Begum S, Banerjee A, De B. Analysis of Antioxidant Activities, Phenolic and Other Metabolites of Some Biomass Waste (Leaves) of India. Free Radicals and Antioxidants. 2018;8(2):102-10. ABSTRACT Background: Antioxidants have important role on human health as well as in food industry. Natural antioxidants are preferred to synthetic antioxidants. Objective: In this study attempt was made to explore the leaf biomass, generally wasted and burnt, as sources of important antioxidant compounds. Methods: Extracts of leaves from five plants e.g. Artocarpus heterophyllus, Haldina cordifolia, Magnolia champaca, Mimusops elengi and Syzygium cumini were analyzed for their antioxidant properties by four different systems of assays. Identification and quantification of important components were performed using GC-MS. Results: All the extracts scavenged DPPH radical, superoxide radical, and chelated metal. S. cumini showed strongest antioxidant activities in all systems of assays. Many important phenols and other metabolites were identified. Correlation between activity and metabolite profile helped to identify important antioxidant compounds in the extracts. Important phenolic antioxidants identified were catechin, epicatechin, protocatechuic acid (from S. cumini), gallic acid, myricetin (from S. cumini, M. elengi), arbutin, 4-hydroxycinnamic acid (from M. elengi). Conclusion: Thus, the leaf biomass may be used as an alternative source of these important phytochemicals having antioxidant activity.
引用本文:Begum S, Banerjee A, De B.一些印度生物质废弃物(叶片)的抗氧化活性和酚类代谢产物的分析。自由基与抗氧化剂[j] . 2018;8(2):102-10。摘要背景:抗氧化剂对人体健康和食品工业有着重要的作用。天然抗氧化剂优于合成抗氧化剂。目的:在本研究中，试图探索叶生物量，一般浪费和燃烧，作为重要的抗氧化化合物的来源。方法:采用四种不同体系的测定方法，对五种植物(石竹、菖蒲、玉兰、菖蒲、小茴香)叶片提取物的抗氧化性能进行分析。采用气相色谱-质谱法对重要成分进行鉴定和定量。结果:所有提取物均清除DPPH自由基、超氧自由基和螯合金属。在各检测体系中，黄颡鱼的抗氧化活性最强。发现了许多重要的酚类物质和其他代谢物。活性与代谢物谱的相关性有助于确定提取物中重要的抗氧化化合物。主要的酚类抗氧化剂有儿茶素、表儿茶素、原儿茶酸(来自茴香树)、没食子酸、杨梅素(来自茴香树)、熊果苷、4-羟基肉桂酸(来自茴香树)。结论:因此，这些具有抗氧化活性的重要植物化学物质可以作为植物生物量的替代来源。
{"title":"Analysis of Antioxidant Activities, Phenolic and Other Metabolites of Some Biomass Waste (Leaves) of India","authors":"Sainiara Begum, A. Banerjee, B. De","doi":"10.5530/FRA.2018.2.16","DOIUrl":"https://doi.org/10.5530/FRA.2018.2.16","url":null,"abstract":"Cite this article: Begum S, Banerjee A, De B. Analysis of Antioxidant Activities, Phenolic and Other Metabolites of Some Biomass Waste (Leaves) of India. Free Radicals and Antioxidants. 2018;8(2):102-10. ABSTRACT Background: Antioxidants have important role on human health as well as in food industry. Natural antioxidants are preferred to synthetic antioxidants. Objective: In this study attempt was made to explore the leaf biomass, generally wasted and burnt, as sources of important antioxidant compounds. Methods: Extracts of leaves from five plants e.g. Artocarpus heterophyllus, Haldina cordifolia, Magnolia champaca, Mimusops elengi and Syzygium cumini were analyzed for their antioxidant properties by four different systems of assays. Identification and quantification of important components were performed using GC-MS. Results: All the extracts scavenged DPPH radical, superoxide radical, and chelated metal. S. cumini showed strongest antioxidant activities in all systems of assays. Many important phenols and other metabolites were identified. Correlation between activity and metabolite profile helped to identify important antioxidant compounds in the extracts. Important phenolic antioxidants identified were catechin, epicatechin, protocatechuic acid (from S. cumini), gallic acid, myricetin (from S. cumini, M. elengi), arbutin, 4-hydroxycinnamic acid (from M. elengi). Conclusion: Thus, the leaf biomass may be used as an alternative source of these important phytochemicals having antioxidant activity.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"30 4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78895696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Nawaz, M. Shad, A. Aziz, S. Muntaha, S. Muzaffar
Cite this article: Nawaz H, Shad MA, Aziz AR, Muntaha ST, Muzaffar S. Phytochemical Composition and Antioxidant Potential of Oven Heated and Microwave Treated Ginger (Zingiber officinale Roscoe). Free Radicals and Antioxidants. 2018;8(2):89-5. ABSTRACT Objective: The study was designed to investigate the effect of heating in the simple oven and microwave treatment on phytochemical composition and antioxidant potential of ginger (Zingiber officinale R.), a popular food ingredient of medicinal importance. Methods: Dried ginger was ground to fine powder and subjected to heating in the simple oven and microwave oven in an increasing order of heating time (5, 10, 15, 20 and 25 min). The heat-treated samples were extracted in methanol and analyzed for their phytochemical composition and antioxidant potential. Results: A time-dependent significant (p<0.05) negative effect of both treatment methods was observed on total phenolic acids (TPA), total tannins content (TTC), total flavonoids content (TFC), total antioxidant activity (TAOA), 2, 2-diphenyl-1-picrylhydrazyl radical scavenging capacity (DPPH RSC) and hydroxyl radical scavenging capacity (HRSC). Both treatments methods showed the significant positive effect on β-carotene bleaching capacity (BCBC), reducing power (RP) and iron chelating activity (ICA). TPA and TAOA of oven heated, TTC and RP of microwave-treated and TFC, BCBC, and HRSC of ginger varied exponentially while TTC and RP of the oven heated, TPA and TAOA of microwave-treated and ICA and DPPH RSC showed linear variation in response to treatment time. Conclusion: Both heating methods have a significant time-dependent effect on the phytochemical composition and antioxidant activity of ginger. This study provides useful information to the manufacturers and consumers regarding the potential benefits of ginger and effect of various heat treatments methods on its medicinal value.
{"title":"Phytochemical Composition and Antioxidant Potential of Oven Heated and Microwave Treated Ginger (Zingiber officinale Roscoe)","authors":"H. Nawaz, M. Shad, A. Aziz, S. Muntaha, S. Muzaffar","doi":"10.5530/FRA.2018.2.14","DOIUrl":"https://doi.org/10.5530/FRA.2018.2.14","url":null,"abstract":"Cite this article: Nawaz H, Shad MA, Aziz AR, Muntaha ST, Muzaffar S. Phytochemical Composition and Antioxidant Potential of Oven Heated and Microwave Treated Ginger (Zingiber officinale Roscoe). Free Radicals and Antioxidants. 2018;8(2):89-5. ABSTRACT Objective: The study was designed to investigate the effect of heating in the simple oven and microwave treatment on phytochemical composition and antioxidant potential of ginger (Zingiber officinale R.), a popular food ingredient of medicinal importance. Methods: Dried ginger was ground to fine powder and subjected to heating in the simple oven and microwave oven in an increasing order of heating time (5, 10, 15, 20 and 25 min). The heat-treated samples were extracted in methanol and analyzed for their phytochemical composition and antioxidant potential. Results: A time-dependent significant (p<0.05) negative effect of both treatment methods was observed on total phenolic acids (TPA), total tannins content (TTC), total flavonoids content (TFC), total antioxidant activity (TAOA), 2, 2-diphenyl-1-picrylhydrazyl radical scavenging capacity (DPPH RSC) and hydroxyl radical scavenging capacity (HRSC). Both treatments methods showed the significant positive effect on β-carotene bleaching capacity (BCBC), reducing power (RP) and iron chelating activity (ICA). TPA and TAOA of oven heated, TTC and RP of microwave-treated and TFC, BCBC, and HRSC of ginger varied exponentially while TTC and RP of the oven heated, TPA and TAOA of microwave-treated and ICA and DPPH RSC showed linear variation in response to treatment time. Conclusion: Both heating methods have a significant time-dependent effect on the phytochemical composition and antioxidant activity of ginger. This study provides useful information to the manufacturers and consumers regarding the potential benefits of ginger and effect of various heat treatments methods on its medicinal value.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"13 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91452899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rohaya Ali, Nasreena Sajjad, S. Qureshi, Sumaya Hassan, Muzaffar Ahmad, S. Ganie, R. Hamid
{"title":"Evaluation of Antioxidant and Immunomodulatory Potential of Methanolic Extract of Juniperus squamata Buch.-Ham. ex D.Don","authors":"Rohaya Ali, Nasreena Sajjad, S. Qureshi, Sumaya Hassan, Muzaffar Ahmad, S. Ganie, R. Hamid","doi":"10.5530/FRA.2018.2.17","DOIUrl":"https://doi.org/10.5530/FRA.2018.2.17","url":null,"abstract":"","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"146 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79140840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Luc Marin, Ellen Carolina Montagner Cavarsan, I. E. Díaz, M. Paciencia, S. Frana, I. Suffredini
1Graduate Program in Environmental and Experimental Pathology, Paulista University, R. Dr. Bacelar, 1212, Vila Clementino, São Paulo, SP, 04026-002, BRAZIL. 2Facultad de Ingeniería Química y Textil de la Universidad Nacional de Ingeniería, Av. Tupac Amaru 210, Rimac, Lima, PERÚ. 3Graduate Program in Dentistry and Center for Research in Biodiversity, Paulista University, Av. Paulista, 900, 1 andar, São Paulo, SP, 01310-100, BRAZIL.
{"title":"Analysis of Brazilian Plant Extracts as Potential Source of Antioxidant Natural Products Using Bench-Top Assays","authors":"Luc Marin, Ellen Carolina Montagner Cavarsan, I. E. Díaz, M. Paciencia, S. Frana, I. Suffredini","doi":"10.5530/FRA.2018.2.18","DOIUrl":"https://doi.org/10.5530/FRA.2018.2.18","url":null,"abstract":"1Graduate Program in Environmental and Experimental Pathology, Paulista University, R. Dr. Bacelar, 1212, Vila Clementino, São Paulo, SP, 04026-002, BRAZIL. 2Facultad de Ingeniería Química y Textil de la Universidad Nacional de Ingeniería, Av. Tupac Amaru 210, Rimac, Lima, PERÚ. 3Graduate Program in Dentistry and Center for Research in Biodiversity, Paulista University, Av. Paulista, 900, 1 andar, São Paulo, SP, 01310-100, BRAZIL.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77248200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"GC- MS, FTIR, UV Analysis and in vitro Antioxidant Activity of a Nigeria Poly Herbal Mixture: Pax Herbal Bitters","authors":"T. Elufioye, Olubunmi O. Mada","doi":"10.5530/FRA.2018.2.12","DOIUrl":"https://doi.org/10.5530/FRA.2018.2.12","url":null,"abstract":"","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79509418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cite this article: Lulan TYK, Fatmawati S, Santoso M, Ersam T. Antioxidant Capacity of Some Selected Medicinal Plants in East Nusa Tenggara, Indonesia: The Potential of Sterculia quadrifida R.Br.. Free Radicals and Antioxidants. 2018;8(2):96-101. ABSTRACT Background: Medicinal plants derived natural product such as alkaloids, flavonoids and terpenes. The traditional uses claim that medicinal plants of NTT are potential folk medicine but very little research has been conducted on these plants. Objective: The present study is directed towards evaluating naturally effective antioxidant of twenty-four traditional medicinal plants collected from Kupang, East Nusa Tenggara (NTT) using in vitro models. Method: The antioxidant activities were determined by using the free radical scavenging assays 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2-2”-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Analysis of total flavonoid and phenolic contents were expressed as Quercetin Equivalent (QE) and Gallic Acid Equivalent (GAE), respectively. Statistical methods are used in data analysis include mean or standard deviation, and regression analysis. Result: Methanol extract of Sterculia quadrifida R. Br. root exhibited the highest DPPH radical scavenging activity with IC50 value of 3.11μg/mL, followed by Schleichera oleosa and Euphorbia hirta L. extracts with IC50 values of 10.05 and 10.09 μg/mL, respectively. Methanol extract of S. quadrifida also showed highest ABTS radical scavenging activity with IC50 value of 7.29 μg/mL, followed by Eugenia jambolana Lam. and Lamea grandus with IC50 value of 9.15 and 12.29 μg/mL, respectively. S. quadrifida extract showed high flavonoid and phenolic content with 661.85 mg of GAE and 116.84 mg of QE per 100 g of extracts. Conclusion: The present study gives scientific evidences that twenty four samples of NTT plants have high free radical scavenging capacity. Strong radical scavenging activity of these plants especially S. quadrifida could be considered as a potential source of natural antioxidants.
{"title":"Antioxidant Capacity of Some Selected Medicinal Plants in East Nusa Tenggara, Indonesia: The Potential of Sterculia quadrifida R.Br.","authors":"T. Y. Lulan, S. Fatmawati, M. Santoso, T. Ersam","doi":"10.5530/FRA.2018.2.15","DOIUrl":"https://doi.org/10.5530/FRA.2018.2.15","url":null,"abstract":"Cite this article: Lulan TYK, Fatmawati S, Santoso M, Ersam T. Antioxidant Capacity of Some Selected Medicinal Plants in East Nusa Tenggara, Indonesia: The Potential of Sterculia quadrifida R.Br.. Free Radicals and Antioxidants. 2018;8(2):96-101. ABSTRACT Background: Medicinal plants derived natural product such as alkaloids, flavonoids and terpenes. The traditional uses claim that medicinal plants of NTT are potential folk medicine but very little research has been conducted on these plants. Objective: The present study is directed towards evaluating naturally effective antioxidant of twenty-four traditional medicinal plants collected from Kupang, East Nusa Tenggara (NTT) using in vitro models. Method: The antioxidant activities were determined by using the free radical scavenging assays 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2-2”-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Analysis of total flavonoid and phenolic contents were expressed as Quercetin Equivalent (QE) and Gallic Acid Equivalent (GAE), respectively. Statistical methods are used in data analysis include mean or standard deviation, and regression analysis. Result: Methanol extract of Sterculia quadrifida R. Br. root exhibited the highest DPPH radical scavenging activity with IC50 value of 3.11μg/mL, followed by Schleichera oleosa and Euphorbia hirta L. extracts with IC50 values of 10.05 and 10.09 μg/mL, respectively. Methanol extract of S. quadrifida also showed highest ABTS radical scavenging activity with IC50 value of 7.29 μg/mL, followed by Eugenia jambolana Lam. and Lamea grandus with IC50 value of 9.15 and 12.29 μg/mL, respectively. S. quadrifida extract showed high flavonoid and phenolic content with 661.85 mg of GAE and 116.84 mg of QE per 100 g of extracts. Conclusion: The present study gives scientific evidences that twenty four samples of NTT plants have high free radical scavenging capacity. Strong radical scavenging activity of these plants especially S. quadrifida could be considered as a potential source of natural antioxidants.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"98 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81212075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Vemuri, R. Banala, Kumar Katragunta, V. Madhuri, K. Raju, Venkata Gurava Reddy Annapareddy, P. Goli
Cite this article: Vemuri SK, Banala RR, Katragunata K, Madhuri V, Raju K, Annapareddy VGR, Goli PVS. Anti-diabetic and Anti-Inflammatory Efficiency of Indian Medicinal Plants against Streptozotocin Induced Diabetes in Male Wistar Rats. Free Radicals and Antioxidants. 2018;8(2):141-8. ABSTRACT Objective: The study was aimed to assess the anti-oxidative and anti-diabetic efficacy of few dietary supplements such as Artocarpus heterophyllus (Raw Jackfruit), Zea mays (Corn Silk), Syzygium cumini (Black plum), and Shilajit (Black asphaltum) in in vitro and in vivo models. The phytoextracts were compared with the known commercial diabetic drugs (Aminoguanidine, Glibenclamide and Insulin) in controlling Streptozotocin induced hyperglycemia, advanced glycation end-products (AGEs), hyperlipidemia and activation of inflammatory mediators (iNOS) in in vivo. Methods: Collection and preparation of phytoextracts, superoxide anion scavenging assay, phosphomolybdate assay (total anti-oxidant capacity), Hydroxyl radical scavenging assay, quantification of hemoglobin glycation, body weights, lipid profile, Pro-inflammatory gene expression, agarose gel electrophoresis and histopathology. Results: We found that natural extracts of A. heterophyllus, S. cumini, Z. mays, and Shilajit exhibited considerable antioxidant capacity and inhibited hemoglobin glycosylation in a dose-dependent manner in in vitro. In STZtreated diabetic rats, the extracts showed clear effects on blood glucose and lipid levels, body weight and pro-inflammatory gene expression (iNOS). Histopathology of treated rat pancreas further supported our in vivo findings. Overall, our study indicates that the natural extracts we tested could be a useful source of antioxidant agents, functional foods and nutraceuticals, particularly in diabetes and related complications. Conclusion: The findings from our study showed reversal of the effects induced by Streptozotocin administration on phytoextracts supplementation in experimental diabetic rats and the therapeutic capacity was similar to that of commercial diabetic drugs, hence these extracts could serve as an alternate in management of diabetes and allied complications.
{"title":"Antioxidant, Anti-Inflammatory and Anti-diabetic Efficiency of Indian Medicinal Plants against Streptozotocin Induced Diabetes in Male Wistar Rats","authors":"S. Vemuri, R. Banala, Kumar Katragunta, V. Madhuri, K. Raju, Venkata Gurava Reddy Annapareddy, P. Goli","doi":"10.5530/FRA.2018.2.21","DOIUrl":"https://doi.org/10.5530/FRA.2018.2.21","url":null,"abstract":"Cite this article: Vemuri SK, Banala RR, Katragunata K, Madhuri V, Raju K, Annapareddy VGR, Goli PVS. Anti-diabetic and Anti-Inflammatory Efficiency of Indian Medicinal Plants against Streptozotocin Induced Diabetes in Male Wistar Rats. Free Radicals and Antioxidants. 2018;8(2):141-8. ABSTRACT Objective: The study was aimed to assess the anti-oxidative and anti-diabetic efficacy of few dietary supplements such as Artocarpus heterophyllus (Raw Jackfruit), Zea mays (Corn Silk), Syzygium cumini (Black plum), and Shilajit (Black asphaltum) in in vitro and in vivo models. The phytoextracts were compared with the known commercial diabetic drugs (Aminoguanidine, Glibenclamide and Insulin) in controlling Streptozotocin induced hyperglycemia, advanced glycation end-products (AGEs), hyperlipidemia and activation of inflammatory mediators (iNOS) in in vivo. Methods: Collection and preparation of phytoextracts, superoxide anion scavenging assay, phosphomolybdate assay (total anti-oxidant capacity), Hydroxyl radical scavenging assay, quantification of hemoglobin glycation, body weights, lipid profile, Pro-inflammatory gene expression, agarose gel electrophoresis and histopathology. Results: We found that natural extracts of A. heterophyllus, S. cumini, Z. mays, and Shilajit exhibited considerable antioxidant capacity and inhibited hemoglobin glycosylation in a dose-dependent manner in in vitro. In STZtreated diabetic rats, the extracts showed clear effects on blood glucose and lipid levels, body weight and pro-inflammatory gene expression (iNOS). Histopathology of treated rat pancreas further supported our in vivo findings. Overall, our study indicates that the natural extracts we tested could be a useful source of antioxidant agents, functional foods and nutraceuticals, particularly in diabetes and related complications. Conclusion: The findings from our study showed reversal of the effects induced by Streptozotocin administration on phytoextracts supplementation in experimental diabetic rats and the therapeutic capacity was similar to that of commercial diabetic drugs, hence these extracts could serve as an alternate in management of diabetes and allied complications.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75115158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Hasaniya, S. Premaratne, Wayne W. Zhang, A. Razzuk, J. Mcnamara, R. Dashwood
1Department of Cardiovascular Surgery, Kaiser Permanente Medical Group, Fontana, California, USA. 2Hunter Holmes McGuire Veterans Administration Medical Centre, Richmond, Virginia, USA. 3Department of Surgery, University of Washington and VA Puget Sounds Healthcare System, Seattle,Washington, USA. 4Department of Surgery, John A. Burns School of Medicine, University of Hawaii and Research Laboratory at the Queen’s Medical Centre, Honolulu, Hawaii, USA. 5Director, Center for Epigenetics and Disease Prevention, Texas A and M College of Medicine, Houston, Texas, USA.
{"title":"Preparation, Characterization and Electron Paramagnetic Resonance (EPR) Spectroscopic Studies of OXANOH","authors":"N. Hasaniya, S. Premaratne, Wayne W. Zhang, A. Razzuk, J. Mcnamara, R. Dashwood","doi":"10.5530/fra.2018.2.11","DOIUrl":"https://doi.org/10.5530/fra.2018.2.11","url":null,"abstract":"1Department of Cardiovascular Surgery, Kaiser Permanente Medical Group, Fontana, California, USA. 2Hunter Holmes McGuire Veterans Administration Medical Centre, Richmond, Virginia, USA. 3Department of Surgery, University of Washington and VA Puget Sounds Healthcare System, Seattle,Washington, USA. 4Department of Surgery, John A. Burns School of Medicine, University of Hawaii and Research Laboratory at the Queen’s Medical Centre, Honolulu, Hawaii, USA. 5Director, Center for Epigenetics and Disease Prevention, Texas A and M College of Medicine, Houston, Texas, USA.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"24 5-6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91503857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Benedetti Serena, Primiterra Mariangela, Finco Annarosa, G. Davide, C. Simona, Battistelli Serafina, Cornelli Umberto
Cite this article: Benedetti S, Primiterra M, Finco A, Gorni D, Catalani S, Battistelli S, U Cornelli. Determination of Plasma Antioxidant Power in Capillary Blood through the Innovative system PAT (Plasma Antioxidant Test). Free Radicals and Antioxidants. 2018;8(2):149-52. ABSTRACT Background: Plasma antioxidants (AOs) represent the main barrier of defense against damaging aggression due to reactive species, by reactive oxygen species (ROS). Moreover, changes in AOs plasma content after supplementation may provide information on the absorption and bioavailability of nutritional compounds and efficacy of AOs therapy. Aim: The aim of this study was to make a comparison between the known BAP test (Biological Antioxidant Potential), used for the evaluation of the antioxidant capacity, and the innovative PAT (Plasma Antioxidant Test), and the evaluation of the precision, linearity and accuracy of the new patented PAT on plasma samples taken from healthy volunteers. Materials and Methods: Twenty-two apparently healthy volunteers were involved in the study for the comparison of the two methods. Results: Analysis performed on 22 plasma samples demonstrated that, following the application of the algorithm, BAP and PAT were interchangeable. PAT resulted extremely precise, linear (R=0.9991), accurate and reproducible (CV 4.17%). PAT values from plasma samples had a normal distribution, evidencing a control range for healthy subjects. Conclusion: PAT can be considered an innovative and predictable method for the measure of the antioxidant power of plasma.
本文来源:Benedetti S, Primiterra M, Finco A, Gorni D, Catalani S, Battistelli S, U Cornelli。创新系统PAT(血浆抗氧化试验)测定毛细管血血浆抗氧化能力。自由基与抗氧化剂研究进展。2018;8(2):149-52。摘要背景:血浆抗氧化剂(AOs)是机体抵御活性氧(ROS)侵袭的主要屏障。此外，补充AOs后血浆含量的变化可以提供营养化合物的吸收和生物利用度以及AOs治疗效果的信息。目的:本研究的目的是比较已知的用于评价抗氧化能力的生物抗氧化电位(BAP)试验和创新的血浆抗氧化试验(PAT)，并评价新专利的PAT对健康志愿者血浆样品的精密度、线性度和准确性。材料与方法:22名表面健康的志愿者参与了两种方法的比较研究。结果:对22份血浆样本的分析表明，应用该算法后，BAP和PAT可互换。PAT结果非常精确、线性(R=0.9991)、准确、可重复性(CV 4.17%)。血浆样本的PAT值呈正态分布，证明健康受试者的控制范围。结论:PAT是一种新颖、可预测的测定血浆抗氧化能力的方法。
{"title":"Determination of Plasma Antioxidant Power in Capillary Blood through the Innovative system PAT (Plasma Antioxidant Test)","authors":"Benedetti Serena, Primiterra Mariangela, Finco Annarosa, G. Davide, C. Simona, Battistelli Serafina, Cornelli Umberto","doi":"10.5530/FRA.2018.2.22","DOIUrl":"https://doi.org/10.5530/FRA.2018.2.22","url":null,"abstract":"Cite this article: Benedetti S, Primiterra M, Finco A, Gorni D, Catalani S, Battistelli S, U Cornelli. Determination of Plasma Antioxidant Power in Capillary Blood through the Innovative system PAT (Plasma Antioxidant Test). Free Radicals and Antioxidants. 2018;8(2):149-52. ABSTRACT Background: Plasma antioxidants (AOs) represent the main barrier of defense against damaging aggression due to reactive species, by reactive oxygen species (ROS). Moreover, changes in AOs plasma content after supplementation may provide information on the absorption and bioavailability of nutritional compounds and efficacy of AOs therapy. Aim: The aim of this study was to make a comparison between the known BAP test (Biological Antioxidant Potential), used for the evaluation of the antioxidant capacity, and the innovative PAT (Plasma Antioxidant Test), and the evaluation of the precision, linearity and accuracy of the new patented PAT on plasma samples taken from healthy volunteers. Materials and Methods: Twenty-two apparently healthy volunteers were involved in the study for the comparison of the two methods. Results: Analysis performed on 22 plasma samples demonstrated that, following the application of the algorithm, BAP and PAT were interchangeable. PAT resulted extremely precise, linear (R=0.9991), accurate and reproducible (CV 4.17%). PAT values from plasma samples had a normal distribution, evidencing a control range for healthy subjects. Conclusion: PAT can be considered an innovative and predictable method for the measure of the antioxidant power of plasma.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75796432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Sinha, B. Kumar, D. Singh, Suaib Luqman, M. Singh, Ashutosh Singh
Cite this article: Sinha S, Kumar B, Singh DK, Luqman S, Singh M, Singh A. Antioxidant and Choline Esterase Inhibitory Activity of Phenolic Rich Extracts from Bombax ceiba L. Flowers. Free Radicals and Antioxidants. 2018;8(2):135-40. ABSTRACT Background: Cognition impairment is the most recurrent form of dementia in aged people indicated by permanent neuronal loss and atypical behaviour. Disparities in cholinergic pathway have been reported as major cause of cognition impairment, where deficiency of acetylcholine occurs due to hydrolysis of acetylcholine by cholinesterases. Bombax ceiba (Bombacaceae) commonly known as silk cotton tree is an imperative plant of tropical and subtropical region which have been mentioned in the traditional systems of medicine. Objectives: We have investigated the cholinesterase and antioxidant activity of B. ceiba extracts. Besides these activity evaluations, preliminary phytochemical testing and quantification of total phenolic and flavonoids have also been performed. Method: Extracts from B. ceiba flowers in hexane and ethanol were prepared by cold maceration method. In vitro cholinesterase activity was estimated with the help of Ellman’s reagent, antioxidant potential of extracts was determined by well known method such as DPPH, FRAP, reducing power and total antioxidant power assay. Total phenolics and flavonoids were quantified by colorimetric method. Results: Finding of present study indicated that B. ceiba extracts are rich in polyphenolic contents (19.10 ± 0.74 QcE and 28.01 ± 1.28 GaE). Results of antioxidative potential evaluation suggested that these extracts have high free radical scavenging potential (65.49 ± 2.49%) and are also able to reduce iron like radicals (93.78 FSE). Beside antioxidant potential B. ceiba extracts also inhibited the cholinesterase effectively (IC50 31.22 ± 1.42 μg/ml). Conclusion: Current investigation on B. ceiba flowers indicated that phenolic rich extracts could be used in development of effective plant-based cholinesterase inhibitors.
{"title":"Antioxidant and Choline Esterase Inhibitory Activity of Phenolic Rich Extracts from Bombax ceiba L. Flowers","authors":"S. Sinha, B. Kumar, D. Singh, Suaib Luqman, M. Singh, Ashutosh Singh","doi":"10.5530/fra.2018.2.20","DOIUrl":"https://doi.org/10.5530/fra.2018.2.20","url":null,"abstract":"Cite this article: Sinha S, Kumar B, Singh DK, Luqman S, Singh M, Singh A. Antioxidant and Choline Esterase Inhibitory Activity of Phenolic Rich Extracts from Bombax ceiba L. Flowers. Free Radicals and Antioxidants. 2018;8(2):135-40. ABSTRACT Background: Cognition impairment is the most recurrent form of dementia in aged people indicated by permanent neuronal loss and atypical behaviour. Disparities in cholinergic pathway have been reported as major cause of cognition impairment, where deficiency of acetylcholine occurs due to hydrolysis of acetylcholine by cholinesterases. Bombax ceiba (Bombacaceae) commonly known as silk cotton tree is an imperative plant of tropical and subtropical region which have been mentioned in the traditional systems of medicine. Objectives: We have investigated the cholinesterase and antioxidant activity of B. ceiba extracts. Besides these activity evaluations, preliminary phytochemical testing and quantification of total phenolic and flavonoids have also been performed. Method: Extracts from B. ceiba flowers in hexane and ethanol were prepared by cold maceration method. In vitro cholinesterase activity was estimated with the help of Ellman’s reagent, antioxidant potential of extracts was determined by well known method such as DPPH, FRAP, reducing power and total antioxidant power assay. Total phenolics and flavonoids were quantified by colorimetric method. Results: Finding of present study indicated that B. ceiba extracts are rich in polyphenolic contents (19.10 ± 0.74 QcE and 28.01 ± 1.28 GaE). Results of antioxidative potential evaluation suggested that these extracts have high free radical scavenging potential (65.49 ± 2.49%) and are also able to reduce iron like radicals (93.78 FSE). Beside antioxidant potential B. ceiba extracts also inhibited the cholinesterase effectively (IC50 31.22 ± 1.42 μg/ml). Conclusion: Current investigation on B. ceiba flowers indicated that phenolic rich extracts could be used in development of effective plant-based cholinesterase inhibitors.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73274748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：