Food microbiology最新文献_第10页

Receptor-specific phage resistance induced phenotypic and gene expression shifts in Salmonella Typhimurium impacting biofilm formation on food contact surfaces 受体特异性噬菌体抗性诱导鼠伤寒沙门菌表型和基因表达改变，影响食物接触表面生物膜的形成

IF 4.6 1区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food microbiology

Pub Date : 2025-09-25 DOI: 10.1016/j.fm.2025.104937

Soo-Jin Jung , Md Ashrafudoulla , Jaewoo Bai , Sang-Do Ha

Bacteriophages are gaining increasing attention as targeted biological control agents against multidrug-resistant (MDR) foodborne pathogens. However, repeated exposure to bacteriophages can induce the emergence of bacteriophage-resistant mutant (BIM) strains, which may exhibit altered physiological characteristics that impair therapeutic efficacy. This study investigated the phenotypic and molecular adaptive mechanisms of multidrug-resistant Salmonella Typhimurium BIMs that acquired resistance to receptor-specific bacteriophages (STP-1 and STP-2) targeting O-antigen and flagella. Two representative BIM strains—MO-4 (O-antigen mutant) and MF-6 (flagella mutant)—were analyzed using phenotypic microarray, motility and biofilm experiments, confocal microscopy, and qRT-PCR. MO-4 exhibited extensive metabolic changes, including 10 alterations in carbon source utilization and increased resistance to 11 antibiotics, while MF-6 showed changes in sensitivity to osmotic and pH stress and increased susceptibility to 5 antibiotics. Both BIM strains exhibited reduced motility but maintained similar or enhanced biofilm formation ability on food contact surfaces. Confocal microscopy analysis revealed structurally distinct biofilms with reduced thickness and increased density. qRT-PCR analysis showed receptor-specific transcriptional changes: MO-4 lost rfaL expression, supporting O-antigen deficiency, while MF-6 showed increased fljB expression and decreased fliK expression, indicating changes in flagellar regulation. Both BIMs showed differences in the expression of tolC, rpoS, and luxS, suggesting changes in efflux, stress response, and quorum sensing. These results highlight receptor-dependent adaptation of phage-resistant Salmonella variants and emphasise the need to evaluate both genetic and phenotypic outcomes when designing effective phage-based control strategies for food safety.

噬菌体作为抗多药耐药（MDR）食源性致病菌的靶向生物控制剂正日益受到关注。然而，反复暴露于噬菌体可诱导出现噬菌体抗性突变（BIM）菌株，其可能表现出改变的生理特性，从而损害治疗效果。本研究探讨了多药耐药鼠伤寒沙门菌（Salmonella Typhimurium BIMs）对靶向o抗原和鞭毛的受体特异性噬菌体（STP-1和STP-2）产生耐药性的表型和分子适应机制。两种具有代表性的BIM菌株mo -4 （o抗原突变体）和MF-6（鞭毛突变体）通过表型微阵列、运动和生物膜实验、共聚焦显微镜和qRT-PCR进行分析。MO-4表现出广泛的代谢变化，包括10种碳源利用的变化和对11种抗生素的耐药性增加，而MF-6表现出对渗透和pH胁迫的敏感性变化，并对5种抗生素的敏感性增加。两种BIM菌株都表现出运动性降低，但在食物接触面上保持相似或增强的生物膜形成能力。共聚焦显微镜分析显示，结构上不同的生物膜厚度减少，密度增加。qRT-PCR分析显示受体特异性转录变化：MO-4缺失ral表达，支持o抗原缺失，而MF-6 fljB表达增加，fliK表达减少，提示鞭毛调控发生变化。两种BIMs在tolC、rpoS和luxS的表达上都存在差异，表明外排、应激反应和群体感应发生了变化。这些结果强调了噬菌体抗性沙门氏菌变体的受体依赖性适应，并强调在设计有效的基于噬菌体的食品安全控制策略时需要评估遗传和表型结果。

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引用次数: 0

Synergistic antibacterial effects and mechanisms of different phenolic acids against Shewanella putrefaciens from fish, with emphasis on the combination of gallic acid and caffeic acid 不同酚酸对鱼源腐坏希瓦氏菌的协同抑菌作用及其机理，重点研究没食子酸和咖啡酸的协同抑菌作用

IF 4.6 1区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food microbiology

Pub Date : 2025-09-24 DOI: 10.1016/j.fm.2025.104940

Qiuying Li , Chenyang Jia , Jiaxing Sun, Tong Sun, Jianrong Li, Xuepeng Li

The strong spoilage capability and cold adaptability of Shewanella putrefaciens present a serious threat to the quality and safety of fish. This study aimed to investigate the synergic antibacterial effects of six phenolic acids (Gallic acid, GA; Caffeic acid, CA; Protocatechuic acid; Ferulic acid; Chlorogenic acid; p-Coumaric acid) in pairs against S. putrefaciens, and to illustrate the antibacterial mechanisms. The fractional inhibitory concentration index (FICI) of 15 combinations of phenolic acids against S. putrefaciens ranged from 0.5 to 0.75, suggesting synergistic or partial synergistic antibacterial effects of these phenolic acids. Time-kill curves and morphologic observation demonstrated that GA and CA (GC) exhibited the strongest synergistic effect against S. putrefaciens. GC synergistically damaged the structure integrity of S. putrefaciens, leading to the leakage of intracellular substances and inhibiting the activity of Na⁺K⁺-ATPase and respiratory chain dehydrogenase. Both GA and CA could bind to DNA and cause changes in DNA content. Proteomics analysis revealed that 399 proteins were affected in the GC groups, a number significantly higher than that in the single GA and CA groups. GC inhibited dissimilatory nitrate and sulfate reduction, and enhanced fatty acid degradation and assimilatory sulfate reduction, which might cause the imbalance of energy supply and ROS accumulation. Meanwhile, the antioxidant-related enzymes, efflux systems and some two-components systems were upregulated, while chemotaxis were inhibited. GC synergistically inhibited S. putrefaciens in turbot, and retarded the spoilage process. The results revealed multi-targeted synergistic antibacterial mechanisms of GC against S. putrefaciens, suggesting GC might be a good strategy for controlling S. putrefaciens and fish spoilage.

腐坏希瓦氏菌具有极强的腐败能力和冷适应性，严重威胁着鱼类的质量安全。本研究旨在研究6种酚酸（没食子酸，GA，咖啡酸，CA，原儿茶酸，阿魏酸，绿原酸，对香豆酸）对腐臭葡萄球菌的协同抑菌作用，并阐明其抑菌机制。15种酚酸组合对腐臭葡萄球菌的分数抑制浓度指数（FICI）在0.5 ~ 0.75之间，表明这些酚酸组合具有协同或部分协同抑菌作用。时间杀伤曲线和形态观察表明，GA和CA （GC）对腐坏葡萄球菌的协同作用最强。GC协同破坏S. putrefaciens的结构完整性，导致胞内物质渗漏，抑制Na+K+- atp酶和呼吸链脱氢酶的活性。GA和CA都能与DNA结合，引起DNA含量的变化。蛋白质组学分析显示，GC组中有399个蛋白受到影响，显著高于单一GA组和CA组。GC抑制了异化性硝酸盐和硫酸盐还原，增强了脂肪酸降解和同化性硫酸盐还原，这可能导致能量供应失衡和ROS积累。同时，抗氧化相关酶、外排系统和部分双组分系统上调，趋化性受到抑制。GC具有协同抑制大比目鱼腐坏链球菌的作用，延缓了大比目鱼的腐坏过程。结果揭示了GC对腐臭葡萄球菌的多靶点协同抑菌机制，提示GC可能是控制腐臭葡萄球菌和鱼类腐败的良好策略。

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引用次数: 0

Resident or transient? Whole-genome approach to tracking colistin-resistant Escherichia coli in the broiler chicken processing chain 常住的还是暂住的？追踪肉鸡加工链中耐粘菌素大肠杆菌的全基因组方法

IF 4.6 1区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food microbiology

Pub Date : 2025-09-24 DOI: 10.1016/j.fm.2025.104939

Ihab Habib , Mohamed-Yousif Ibrahim Mohamed , Glindya Bhagya Lakshmi , Akela Ghazawi , Mushtaq Khan

This study presents a genome-informed surveillance model to investigate the persistence and spread of colistin-resistant Escherichia coli in broiler chicken processing. The study targeted a high-throughput poultry facility—previously linked to retail meat contamination by colistin-resistant E. coli and Salmonella—where 200 carcasses were sampled across ten production batches to assess the prevalence and genomic characteristics of antimicrobial-resistant strains within the processing line. We analyzed one E. coli isolate per carcass to characterize antimicrobial resistance (AMR), and utilized whole-genome sequencing (WGS) to delineate phylogeny, virulence, AMR determinants, and plasmid content. Colistin-resistant E. coli isolates were detected in all production batches and were confirmed in 10.5 % (21/200) of the carcasses, with all isolates carrying the mcr-1.1 gene. Notably, 57.1 % of these isolates also harbored a PmrB Y358N putative colistin resistance mutation. Phylogenetic analysis revealed substantial diversity, with 31 sequence types detected; however, six isolates belonging to ST162 were identified as a resident strains cluster, persisting over four months and from multiple farms and flocks. All colistin-resistant E. coli isolates were phenotypically multidrug-resistant (MDR), carrying 10–25 AMR resistance genes per genome, including ESBL genes such as bla_CTX-M-55 (57.1 %). Virulence profiling showed a high prevalence of iron acquisition, serum resistance, and efflux-related genes, with an average of 22.5 putative virulence factors per isolate. Plasmidome analysis (n = 20 plasmids) revealed the dominance of IncI2 (60 %) and IncHI2-type replicons, with 90 % of plasmids predicted to be conjugative. Mobile genetic elements involved in horizontal gene transfer, such as MOBP relaxases and MPF-T systems, were prevalent (70 %), indicating a high potential for plasmid-mediated dissemination of AMR genes within the sampled isolates. This work offers a scalable model for processing facility-level AMR tracking and reinforces the value of WGS for industry-led food safety risk management, particularly for high-priority AMR determinants such as colistin resistance.

本研究提出了一种基因组监测模型，以调查肉鸡加工过程中耐粘菌素大肠杆菌的持久性和传播。这项研究的目标是一个高吞吐量的家禽设施——以前与耐粘菌素的大肠杆菌和沙门氏菌的零售肉类污染有关——在那里，对10个生产批次的200具尸体进行了取样，以评估加工生产线中耐抗生素菌株的流行程度和基因组特征。我们分析了每具大肠杆菌胴体的一个分离株来表征抗菌素耐药性（AMR），并利用全基因组测序（WGS）来描述系统发育、毒力、AMR决定因素和质粒含量。在所有生产批次中均检测到耐粘菌素大肠杆菌分离株，在10.5%（21/200）的胴体中检测到，所有分离株均携带mcr-1.1基因。值得注意的是，57.1%的分离株还携带PmrB Y358N假定的粘菌素耐药突变。系统发育分析显示了丰富的多样性，共检测到31种序列类型；然而，6株属于ST162的分离株被确定为常驻菌株群，持续时间超过4个月，来自多个农场和鸡群。所有耐粘菌素大肠杆菌分离株均表现为表型多重耐药（MDR），每个基因组携带10-25个AMR耐药基因，包括ESBL基因，如blaCTX-M-55（57.1%）。毒力分析显示铁获取、血清抗性和外排相关基因的高流行率，每个分离物平均有22.5个推定毒力因子。质粒分析（n = 20个质粒）显示inc2型和inc2型复制子占主导地位（60%），预计90%的质粒为共轭质粒。参与水平基因转移的移动遗传元件，如MOBP松弛和MPF-T系统，普遍存在（70%），表明AMR基因在样本分离物中质粒介导的传播潜力很大。这项工作为加工设施级别的抗菌素耐药性跟踪提供了一个可扩展的模型，并加强了WGS在行业主导的食品安全风险管理中的价值，特别是对于诸如粘菌素耐药性等高优先级抗菌素耐药性决定因素。

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引用次数: 0

Evaluation of Cecure as a postharvest sanitizer for controlling Listeria monocytogenes on fresh apples Cecure采后杀菌剂对鲜苹果单核细胞增生李斯特菌的防治效果评价

IF 4.6 1区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food microbiology

Pub Date : 2025-09-22 DOI: 10.1016/j.fm.2025.104936

Yuan Su, Xiaoye Shen, Jian Cong, Mei-Jun Zhu

Recent listeriosis outbreaks linked to fresh apples highlight the need to control Listeria monocytogenes during postharvest handling. Commercially used sanitizers have shown limited efficacy against L. monocytogenes on apple surfaces, indicating a need for alternative interventions. This study evaluated the efficacy of Cecure, a generally recognized as safe (GRAS) antimicrobial solution, in controlling Listeria monocytogenes in solutions, on inoculated apples, and preventing cross-contamination. Cecure demonstrated minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of 8 and 16 ppm, respectively, against three L. monocytogenes strains. In solution, Cecure demonstrated strong anti-Listeria efficacy, resulting in reductions of 3.7–6.3 log CFU/ml in 30 s at 16–128 ppm. On inoculated apples, a 0.5-min wash with 0.0125–1.6 % Cecure resulted in 0.4–2.0 log CFU/apple reduction of L. monocytogenes. Increasing the contact time to 2-min significantly improved the anti-Listeria efficacy of 0.8–1.6 % Cecure, achieving reductions of 2.1–2.5 log CFU/apple. In addition, Cecure effectively prevented apple-to-apple cross-contamination at 0.8–1.6 % Cecure during 0.5 min wash. Additionally, Cecure reduced apple resident bacteria by 1.0–1.4 log CFU/apple and yeasts and molds by 1.3–1.8 log CFU/apple. These results suggest that Cecure may serve as a potential alternative sanitizer for reducing L. monocytogenes contamination risks during postharvest apple processing.

最近与新鲜苹果有关的李斯特菌病暴发突出了在采收后处理过程中控制单核细胞增生李斯特菌的必要性。商业上使用的杀菌剂对苹果表面单核细胞增生乳杆菌的效果有限，表明需要其他干预措施。本研究评估了公认的安全（GRAS）抗菌溶液Cecure在控制溶液中单核细胞增生李斯特菌、接种苹果和防止交叉污染方面的效果。Cecure对3株单核增生乳杆菌的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）分别为8 ppm和16 ppm。在溶液中，Cecure表现出很强的抗李斯特菌效果，在16-128 ppm条件下，30秒内降低3.7-6.3 log CFU/ml。在接种后的苹果上，用0.0125 - 1.6%的Cecure洗涤0.5 min，单核细胞增生乳杆菌数量减少0.4-2.0 log CFU/苹果。将接触时间延长至2 min，可显著提高甜菜抗李斯特菌效果0.8 ~ 1.6%，降低2.1 ~ 2.5 log CFU/苹果。此外，Cecure在0.5 min的洗涤时间内有效地防止了苹果与苹果之间的交叉污染，Cecure含量为0.8 - 1.6%。此外，Cecure使苹果的常驻细菌减少1.0-1.4 log CFU/苹果，酵母和霉菌减少1.3-1.8 log CFU/苹果。这些结果表明，在苹果采后加工过程中，Cecure可能作为一种潜在的替代消毒剂来降低单核细胞增生乳杆菌的污染风险。

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引用次数: 0

Trichoderma asperellum 2-X-3-3 as a sustainable biocontrol alternative to fungicides for postharvest pear diseases 曲霉2-X-3-3作为杀菌剂对梨采后病害的可持续生物防治

IF 4.6 1区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food microbiology

Pub Date : 2025-09-20 DOI: 10.1016/j.fm.2025.104934

Xi Xu , Chunbo Yang , Ziyun Xu , Chenyang Jiao , Xilang Yang , Yiwen Wang , Siqi Ma , Wensheng Xiang , Xiangjing Wang , Junwei Zhao

Postharvest black spot disease caused by Alternaria alternata severely impacted pear storage and transportation, leading to substantial economic losses. Biological control, particularly with Trichoderma species, offers a sustainable and effective alternative to chemical fungicides. In this study, Trichoderma asperellum 2-X-3-3, isolated from greenhouse soil used for morel cultivation, exhibited strong antifungal activity against A. alternata, achieving a 74.4 % inhibition rate through producing volatile organic compounds (VOCs). These VOCs inhibited A. alternata by reducing conidial germination and sporulation, disrupting mycelial structures, and damaging cell membrane integrity. Moreover, they also improved pear quality, boosted antioxidant enzyme activity, and upregulated gene expression, significantly extending storage time. HS-GC-IMS analysis identified 15 VOCs, all of which exhibited antifungal activity against A. alternata. Among them, seven VOCs demonstrated significant inhibitory activity (>50 %), with three (3-octanone, acetic acid, and 1-hexanol) achieving nearly 100 % inhibition at 0.5 mL/L, highlighting their critical role in pathogen suppression. These findings demonstrate T. asperellum 2-X-3-3 as a potential, sustainable alternative to chemical fungicides for managing postharvest pear black spot disease, improving fruit quality, and extending shelf life.

梨采后黑斑病严重影响梨的储运，造成巨大的经济损失。生物防治，特别是木霉防治，是化学杀菌剂的一种可持续和有效的替代方法。本研究从羊粪栽培温室土壤中分离得到的曲霉2-X-3-3，通过产生挥发性有机物（VOCs），对草霉表现出较强的抑菌活性，抑菌率达74.4%。这些挥发性有机化合物通过减少分生孢子萌发和产孢，破坏菌丝结构和破坏细胞膜完整性来抑制交替孢。此外，它们还改善了梨的品质，提高了抗氧化酶活性，上调了基因表达，显著延长了贮藏时间。HS-GC-IMS分析鉴定出15种挥发性有机化合物，均表现出抗真菌活性。其中，7种挥发性有机化合物表现出显著的抑制活性（50%），其中3-辛酮、乙酸和1-己醇在0.5 mL/L浓度下的抑制活性接近100%，突出了它们在抑制病原体中的重要作用。这些研究结果表明，曲霉2-X-3-3是一种潜在的、可持续的化学杀菌剂替代品，可用于控制采后梨黑斑病，改善果实品质，延长保质期。

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引用次数: 0

Antifungal activity and action mechanism of Bacillus velezensis against Phyllosticta capitalensis isolated from guava fruits 番石榴芽孢杆菌对毛缕虫的抑菌活性及作用机制研究

IF 4.6 1区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food microbiology

Pub Date : 2025-09-19 DOI: 10.1016/j.fm.2025.104935

Wei Deng , Shan Wang , Zehao Yang , Haoliang Liu , Yuhang Chen , Danxian Shen , Ting Fang , Tuanwei Chen

The antifungal mechanism of Bacillus velezensis (B. velezensis) against Phyllosticta capitalensis (P. capitalensis), a pathogen isolated from guava fruits and its efficacy in controlling guava black spot disease were systematically investigated. The minimum inhibitory concentration (MIC) of B. velezensis against P. capitalensis was identified to be 10⁴ CFU/mL. Microscopic observations revealed significant morphological alterations in P. capitalensis mycelium following B. velezensis treatment, suggesting compromised cell membrane integrity and a consequent increase in membrane permeability. Moreover, B. velezensis treatment induced elevated levels of malondialdehyde (MDA), hydrogen peroxide (H₂O₂) and superoxide anions (O₂•^-), accompanied by reduced catalase (CAT) and peroxidase (POD) activities in P. capitalensis, thereby effectively inhibiting its growth. Transcriptome analysis provided further evidence that B. velezensis might exert its antifungal effects through destabilizing the cell membrane and attenuating the antioxidant defense capacity of P. capitalensis. B. velezensis treatment significantly slowed the disease development caused by P. capitalensis in guavas, with diameters measuring only 0.097 cm at 4 days and 0.423 cm at 8 days, which substantially lower than those of the CK group (P < 0.01). These findings indicate that B. velezensis holds considerable potential as an effective biocontrol agent for managing guava black spot disease.

系统研究了velezensis芽孢杆菌对番石榴果实分离病原菌capitalphyllosticta （P. capitalensis）的抑菌机制及其对番石榴黑斑病的防治效果。结果表明，该菌株对大写P. capitalensis的最低抑菌浓度为104 CFU/mL。显微镜观察显示，白僵菌处理后，白僵菌菌丝的形态发生了显著变化，表明细胞膜完整性受损，膜通透性随之增加。此外，B. velezensis处理导致草木丙二醛（MDA）、过氧化氢（H2O2）和超氧阴离子（O2•-）水平升高，过氧化氢酶（CAT）和过氧化物酶（POD）活性降低，从而有效抑制草木生长。转录组分析进一步证明，白僵菌可能是通过破坏白僵菌细胞膜的稳定和削弱白僵菌的抗氧化防御能力来发挥抗真菌作用的。B. velezensis处理显著减缓了番石榴病的发展，4 d时直径仅为0.097 cm， 8 d时直径仅为0.423 cm，显著低于CK组（P < 0.01）。这些结果表明，velezensis作为番石榴黑斑病的有效生物防治剂具有相当大的潜力。

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引用次数: 0

Application of pascalisation in the control of Listeria sp. in beverages – Effectiveness, limitations, and future prospects pascalisation技术在饮料中李斯特菌控制中的应用——有效性、局限性和未来展望

IF 4.6 1区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food microbiology

Pub Date : 2025-09-16 DOI: 10.1016/j.fm.2025.104932

Patryk Wiśniewski, Anna Zadernowska

Pascalisation is one of the non-thermal methods for reducing contamination of beverages with Listeria monocytogenes and Listeria innocua. This article examines the effectiveness of pascalisation in eliminating these bacteria in beverages, analyzing the impact of process parameters (pressure value, dwell time, temperature), its limitations, and the potential for synergy with other methods based on studies from the Scopus and Web of Science databases. Although industrial applications typically use the pressure of 600 MPa, many studies have reported bacterial inactivation at lower pressures. Reducing the pressure in pascalisation may seem advantageous for preserving the product's sensory qualities, but it does not necessarily ensure appropriate microbiological safety. This is due to the barotolerance of some Listeria sp. strains, the risk of forming viable but non-culturable cells (VBNC) undetectable by standard methods, and the possibility of microbial regrowth after processing. Additionally, high pressure, acting as a stressor, induces DNA damage and the SOS response and increases cell membrane permeability, affecting changes and transfer of antibiotic resistance genes (ARG). Growing market demand for plant-based, minimally processed beverages drives the search for economically viable solutions that combine high-pressure processing with other preservation methods. Current research explores combining pascalisation with different hurdles to reduce pressure requirements without compromising microbiological safety. Nevertheless, acidic matrices remain more suitable than neutral or antioxidant-rich formulations, which show decreased bacterial inactivation. Future research should refine pascalisation parameters for various beverage types and explore synergistic preservation strategies to ensure appropriate microbiological quality while preserving sensory and nutritional properties.

pascalization是一种减少饮料中单核细胞增生李斯特菌和无害李斯特菌污染的非热方法。本文基于Scopus和Web of Science数据库的研究，研究了pascalisation在消除饮料中这些细菌方面的有效性，分析了工艺参数（压力值，停留时间，温度）的影响，其局限性以及与其他方法协同作用的潜力。虽然工业应用通常使用600兆帕的压力，但许多研究报道了在较低压力下细菌灭活的情况。降低paspasization的压力似乎有利于保持产品的感官质量，但它不一定确保适当的微生物安全性。这是由于一些李斯特菌菌株的耐压性，形成标准方法无法检测到的活的但不可培养的细胞（VBNC）的风险，以及处理后微生物再生的可能性。此外，高压作为应激源，诱导DNA损伤和SOS反应，增加细胞膜通透性，影响抗生素耐药基因（ARG）的变化和转移。市场对植物性、最低限度加工饮料的需求不断增长，促使人们寻求经济可行的解决方案，将高压加工与其他保存方法相结合。目前的研究探索将pascalization与不同的障碍结合起来，在不影响微生物安全性的情况下降低压力要求。然而，酸性基质仍然比中性或富含抗氧化剂的配方更合适，它们显示出细菌失活的减少。未来的研究应完善各种饮料的pascalized参数，并探索协同保存策略，以确保适当的微生物质量，同时保持感官和营养特性。

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引用次数: 0

Functional properties of autochthonous lactic acid bacteria starter cultures for plant-based food fermentation 植物性食品发酵中原生乳酸菌发酵剂的功能特性

IF 4.6 1区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food microbiology

Pub Date : 2025-09-12 DOI: 10.1016/j.fm.2025.104926

Charlotte Bauer Munch-Andersen, Tove Gulbrandsen Devold, Davide Porcellato, Hilde Marit Østlie

Lactic acid bacteria (LAB) that are autochthonous to the raw material are commonly recognised as the most suitable bacteria for starter cultures for fermentation. In this study, 172 LAB isolated from cereals and pulses were genetically characterised, and 37 of these were whole genome sequenced. Twenty-four LAB were selected for technological characterization. Screening assays were set up to determine the ability of these isolates to produce exopolysaccharides and hydrolyse proteins, reduce phytic acid, and ferment various carbohydrate substrates. The ability to grow and acidify was tested in flour-like media and flour slurries. Chromatographic methods were applied to quantify carbohydrates and organic acids before and after fermentation. The 24 isolates belonging to 8 different species of LAB exhibited a diverse combination of important functional and technological properties.

Isolates with the ability to rapidly reduce pH, proliferate at a high rate, produce large amounts of organic acids and metabolise citrate were found among the 24 candidates. The isolates displayed different combinations of carbohydrate fermentation ability, proteolytic activity, phytase activity, and formation of mucoid and threading colonies. Several isolates exhibited one or more desirable traits, with one expressing all the desired properties tested here; Lpb. paraplantarum JP51. These results provide insights into the potential of these isolates for developing autochthonous starter cultures.

乳酸菌（LAB）是原料的原生细菌，通常被认为是最适合发酵发酵剂的细菌。本研究对从谷物和豆类中分离的172株乳酸菌进行了遗传鉴定，并对其中37株进行了全基因组测序。选取24个LAB进行工艺表征。筛选试验确定了这些分离株产生外多糖和水解蛋白质、还原植酸和发酵各种碳水化合物底物的能力。在类面粉培养基和面粉浆料中测试了其生长和酸化能力。采用色谱法定量发酵前后的碳水化合物和有机酸。来自8个不同种的24株乳酸菌表现出重要的功能和工艺特性的多样化组合。在24个候选菌株中，分离菌株具有快速降低pH值、快速增殖、产生大量有机酸和代谢柠檬酸的能力。分离菌株在碳水化合物发酵能力、蛋白水解活性、植酸酶活性以及粘液和穿线菌落形成方面表现出不同的组合。一些分离株表现出一种或多种理想性状，其中一种表现出本文所测试的所有理想性状；有限目的银行模式。paraplantarum JP51。这些结果为这些分离株发展本土发酵剂的潜力提供了见解。

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引用次数: 0

Effect of surfactants on the antimicrobial activity of hydrogen peroxide and chlorine dioxide foams against Listeria monocytogenes on stainless steel 表面活性剂对双氧水和二氧化氯泡沫对不锈钢表面李斯特菌抑菌活性的影响

IF 4.6 1区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food microbiology

Pub Date : 2025-09-11 DOI: 10.1016/j.fm.2025.104929

Seoyoung Yoon , Nahyun Kim , Larry R. Beuchat , Hoikyung Kim , Jee-Hoon Ryu

This study evaluated the antilisterial activity of hydrogen peroxide (H₂O₂) and chlorine dioxide (ClO₂) in foam formulations containing various surfactants against Listeria monocytogenes on stainless steel coupons (SSCs). First, the minimum inhibitory and lethal concentrations of six surfactants (sodium dodecyl sulfate [SDS], cetylpyridinium chloride [CPC], cetyltrimethylammonium bromide [CTAB], coco betaine, Triton X-100, and Tween 80) were determined using planktonic cells, with CPC and CTAB exhibiting the highest activity. Second, surfactant solutions (100 μg/mL) were applied to L. monocytogenes attached to SSCs to assess surface-associated antimicrobial effects. CPC and CTAB again showed the greatest reductions, by 2.4 and 2.7 log CFU/coupon, respectively. Third, H₂O₂ (3 %) and ClO₂ (200 μg/mL) were tested alone or in combination with surfactant foams against the attached cells on SSCs. H₂O₂+CPC and H₂O₂+CTAB foams yielded the highest reductions (2.4 and 2.1 log CFU/coupon, respectively), demonstrating enhanced reductions by combination treatments compared with individual treatments. ClO₂+CPC and ClO₂+SDS foams resulted in 2.6 and 2.2 log reductions, respectively, with greater effects observed than for the individual treatments. In contrast, combinations with coco betaine, Triton X-100, or Tween 80 were less effective than H₂O₂ or ClO₂ alone. Finally, the selected foams were evaluated against L. monocytogenes biofilms formed on SSC. These findings demonstrate that the antimicrobial efficacy of H₂O₂ and ClO₂ foams can be significantly enhanced through appropriate surfactant selection, particularly against planktonic and surface-attached L. monocytogenes, while also highlighting the need for improved strategies to control biofilm-associated cells.

本研究评价了含不同表面活性剂的泡沫制剂对不锈钢板（ssc）上单核增生李斯特菌的抑菌活性。首先，利用浮游细胞测定了六种表面活性剂（十二烷基硫酸钠[SDS]、十六烷基氯化吡啶[CPC]、十六烷基三甲基溴化铵[CTAB]、可可甜菜碱、Triton X-100和Tween 80）的最小抑制浓度和致死浓度，其中CPC和CTAB表现出最高的活性。其次，将表面活性剂溶液（100 μg/mL）应用于附着在SSCs上的单核增生乳杆菌，以评估其表面相关的抗菌作用。CPC和CTAB再次显示出最大的减少，分别减少2.4和2.7 log CFU/coupon。第三，分别用H2O2（3%）和ClO2 （200 μg/mL）单独或联合表面活性剂泡沫对ssc上附着细胞的作用。H2O2+CPC和H2O2+CTAB泡沫的降低率最高（分别为2.4和2.1 log CFU/coupon），表明与单独处理相比，联合处理的降低率更高。ClO2+CPC和ClO2+SDS泡沫分别减少了2.6和2.2对数，比单独处理的效果更大。相比之下，与可可甜菜碱、Triton X-100或Tween 80联合使用的效果不如单独使用H2O2或ClO2。最后，将所选泡沫与SSC上形成的单核增生乳杆菌生物膜进行对比。这些发现表明，通过适当的表面活性剂选择，H2O2和ClO2泡沫的抗菌效果可以显著增强，特别是对浮游和表面附着的单核细胞增生乳杆菌，同时也强调了改进控制生物膜相关细胞的策略的必要性。

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引用次数: 0

Evaluation of BioFire® FilmArray® Gastrointestinal panel for the detection of norovirus in complex food matrices BioFire®FilmArray®胃肠道面板用于复杂食品基质中诺如病毒检测的评价

IF 4.6 1区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food microbiology

Pub Date : 2025-09-10 DOI: 10.1016/j.fm.2025.104931

Stéphanie Watier-Grillot , Julie Loutreul , Nicolas Boudaud , Christian Sauvageot , Evelyne Thiébaut , Vincent Pommier de Santi , Aurélie Chesnay

Noroviruses (NoVs) are a leading cause of foodborne diseases (FBDs). Detecting infectious NoV in food is not possible because cell culture approaches are not sensitive enough and compatible with such complex matrices. For this reason, the available standard methods that can be used in routine screening only enable the detection of NoV genome in food, without distinguishing between infectious and non-infectious viruses. Moreover, NoV FBDs commonly involve other microorganisms (viruses, bacteria), which complicates diagnosis and investigation, hence the advantage of using sensitive multiplex PCR-based methods. The BioFire® FilmArray® Gastrointestinal Panel (BF-GIP) was evaluated as an alternative method for detecting NoV genome in food, compared to the ISO 15216-2 standard method and in compliance with ISO standard 16140–2. A total of 60 vegetable-based food samples were tested. BF-GIP sensitivity (62.7 %) was close to that of the standard method (64.4 %). BF-GIP generated a false positive rate of 10 %. For the overall level of detection, no significant difference was shown between BF-GIP and the standard method. Food sample processing methods that generate high virus recovery rates are essential to improving NoV detection with BF-GIP.

诺如病毒（NoVs）是食源性疾病（fbd）的主要原因。由于细胞培养方法不够敏感，而且与如此复杂的基质不兼容，因此无法检测食品中的传染性NoV。因此，现有的用于常规筛查的标准方法只能检测食品中的NoV基因组，无法区分传染性和非传染性病毒。此外，NoV fbd通常涉及其他微生物（病毒、细菌），这使诊断和调查复杂化，因此使用敏感的基于多重pcr的方法具有优势。与ISO 15216-2标准方法相比，BioFire®FilmArray®胃肠道检测面板（BF-GIP）作为检测食品中NoV基因组的替代方法进行了评估，并符合ISO 16140-2标准。共测试了60个蔬菜类食品样本。BF-GIP的灵敏度（62.7%）与标准方法（64.4%）接近。BF-GIP产生的假阳性率为10%。在整体检测水平上，BF-GIP与标准方法无显著差异。产生高病毒回收率的食品样品处理方法对于提高用BF-GIP检测NoV至关重要。

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引用次数: 0