Yuniel Méndez-Martínez, Alan Rodrigo Vera-Veliz, Edilmar Cortés-Jacinto, Yanis Cruz-Quintana, Aroldo Botello-Leon, Pedro Daniel Mendoza-Carranza, Natalia S. Calvo
This study aimed to evaluate the growth performance, feed utilisation, digestive and metabolic enzyme activity, and liver histology in juveniles of hybrid red tilapia (Oreochromis mossambicus × Oreochromis niloticus) fed with the inclusion of chitosan in their diet. Six dietary chitosan levels (0 “control’’, 10, 20, 30, 40, and 50 g kg−1) were used to feed juvenile fish (initial weight 7.50 ± 0.20 g) that were cultured for eight weeks in 18 tanks at a density of 15 fish/tank. The growth performance presented significant differences (p ˂ 0.05) for weight gain, specific growth rate, feed conversion ratio, hepatosomatic index, and survival rate. The digestive and metabolic enzyme activities were significantly (p < 0.05) influenced by the levels of chitosan in the administered diet. Histologically, no damage was found in the liver; however, morphometrically, a significant difference (p < 0.05) was found in the hepatocyte area and sinusoid area. Vacuolisation of hepatocytes was found in treatments with 40 and 50 g kg−1 of chitosan in the diet. Treatments with doses of chitosan showed a better response (p < 0.05) compared to the control treatment in most of the different groups of variables analysed. The result of the principal component analysis suggests that a diet containing 40 g kg−1 of chitosan is optimal for tilapia growth performance.
本试验旨在研究在饲料中添加壳聚糖对杂交红罗非鱼幼鱼(Oreochromis mossambicus × Oreochromis niloticus)生长性能、饲料利用率、消化代谢酶活性和肝脏组织学的影响。试验采用6种不同水平的壳聚糖(0“对照”、10、20、30、40和50 g kg - 1)饲喂初始体重为7.50±0.20 g的幼鱼，这些幼鱼在18个水族箱中以15条/水族箱的密度养殖8周。生长性能在增重、特定生长率、饲料系数、肝体指数和成活率方面存在显著差异(p小于0.05)。消化代谢酶活性显著提高(p <0.05)，受饲粮中壳聚糖含量的影响。组织学上，肝脏未见损伤;然而，形态计量学上，显著差异(p <肝细胞区和窦区可见0.05)。在日粮中添加40和50 g kg - 1壳聚糖时，肝细胞出现空泡化。壳聚糖治疗效果更好(p <0.05)，与对照组相比，在大多数不同组的变量分析。主成分分析结果表明，壳聚糖的添加量为40 g kg−1，有利于罗非鱼的生长性能。
{"title":"Growth Performance, Feed Utilisation, Digestive and Metabolic Enzyme Activity, and Liver Morphohistology in Hybrid Tilapia (Oreochromis mossambicus × Oreochromis niloticus) Juveniles Fed with the Inclusion of Chitosan in Their Diet","authors":"Yuniel Méndez-Martínez, Alan Rodrigo Vera-Veliz, Edilmar Cortés-Jacinto, Yanis Cruz-Quintana, Aroldo Botello-Leon, Pedro Daniel Mendoza-Carranza, Natalia S. Calvo","doi":"10.3390/fishes8110546","DOIUrl":"https://doi.org/10.3390/fishes8110546","url":null,"abstract":"This study aimed to evaluate the growth performance, feed utilisation, digestive and metabolic enzyme activity, and liver histology in juveniles of hybrid red tilapia (Oreochromis mossambicus × Oreochromis niloticus) fed with the inclusion of chitosan in their diet. Six dietary chitosan levels (0 “control’’, 10, 20, 30, 40, and 50 g kg−1) were used to feed juvenile fish (initial weight 7.50 ± 0.20 g) that were cultured for eight weeks in 18 tanks at a density of 15 fish/tank. The growth performance presented significant differences (p ˂ 0.05) for weight gain, specific growth rate, feed conversion ratio, hepatosomatic index, and survival rate. The digestive and metabolic enzyme activities were significantly (p < 0.05) influenced by the levels of chitosan in the administered diet. Histologically, no damage was found in the liver; however, morphometrically, a significant difference (p < 0.05) was found in the hepatocyte area and sinusoid area. Vacuolisation of hepatocytes was found in treatments with 40 and 50 g kg−1 of chitosan in the diet. Treatments with doses of chitosan showed a better response (p < 0.05) compared to the control treatment in most of the different groups of variables analysed. The result of the principal component analysis suggests that a diet containing 40 g kg−1 of chitosan is optimal for tilapia growth performance.","PeriodicalId":12405,"journal":{"name":"Fishes","volume":" 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135242082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Estuaries are extremely productive ecosystems, providing habitats for numerous aquatic species and crucial ecological services. The Tejo estuary, one of the largest European estuaries, has been thoroughly studied, and its important functional role as a nursery for several commercially important fish species is already established. In the present work, a trait-based approach was applied to functionally describe the fish community structure of the Tejo estuary and to enlighten potential changes in the ecosystem functioning at this level, following environmental changes expected to occur. To predict the distribution of species from the two most representative ecological guilds of the Tejo estuary, estuarine residents and marine migrants, species distribution models were built using an ensemble technique (combining forecasts of single models). The predictions obtained were more accurate for the marine migrants and the species distribution was strongly related with salinity, whereas estuarine species, were also influenced by depth, habitat type and river flow. The potential distributions of these ecological guilds showed that marine migrants will tend to use upstream areas in the estuary, where salinity is lower. Nonetheless, salinity is expected to increase as extreme weather events such as droughts tend to occur more frequently, decreasing favorable habitat availability for these species, and thus threatening the crucial role this ecosystem plays for these species.
{"title":"Changes in the Functional Role of the Tejo Estuary (Portugal, Europe) According to Fish Ecological Guilds","authors":"Susana França","doi":"10.3390/fishes8110545","DOIUrl":"https://doi.org/10.3390/fishes8110545","url":null,"abstract":"Estuaries are extremely productive ecosystems, providing habitats for numerous aquatic species and crucial ecological services. The Tejo estuary, one of the largest European estuaries, has been thoroughly studied, and its important functional role as a nursery for several commercially important fish species is already established. In the present work, a trait-based approach was applied to functionally describe the fish community structure of the Tejo estuary and to enlighten potential changes in the ecosystem functioning at this level, following environmental changes expected to occur. To predict the distribution of species from the two most representative ecological guilds of the Tejo estuary, estuarine residents and marine migrants, species distribution models were built using an ensemble technique (combining forecasts of single models). The predictions obtained were more accurate for the marine migrants and the species distribution was strongly related with salinity, whereas estuarine species, were also influenced by depth, habitat type and river flow. The potential distributions of these ecological guilds showed that marine migrants will tend to use upstream areas in the estuary, where salinity is lower. Nonetheless, salinity is expected to increase as extreme weather events such as droughts tend to occur more frequently, decreasing favorable habitat availability for these species, and thus threatening the crucial role this ecosystem plays for these species.","PeriodicalId":12405,"journal":{"name":"Fishes","volume":"356 4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135392584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhe Zhang, Chengkuan Lu, Kebing Lin, Weiwei You, Zhangwu Yang
The whiteleg shrimp (Litopenaeus vannamei) is one of the most economically important aquaculture species in China. Genetic diversity is crucial for maintaining the gene pool of farmed shrimp. In this study, the effects of artificial selection on the genetic structure of four whiteleg shrimp strains were evaluated using microsatellite sequences. The results showed that the observed heterozygosity (Ho), expected heterozygosity (He), and the polymorphism information content (PIC) of the four selected strains ranged from 0.446 to 0.574, 0.450 to 0.566, and 0.435 to 0.509, respectively. All the selected strains maintained a moderate level of genetic diversity. Most inbreeding coefficients (Fis) in the four strains were positive but not significantly different from zero, indicating a relatively low degree of inbreeding within each strain. However, He and PIC in line 5 showed a decreasing trend from the 2017 to the 2019 generations, and Fis in line 10 showed a significant increasing trend across generations, indicating that measures must be taken to maintain the level of genetic diversity for lines 5 and 10. UPGMA cluster trees showed that the four breeding lines had apparent genetic differences, which could provide a genetic basis for studying crossbreeding between selective lines and the utilization of heterosis. This study will be useful for population genetic research and the breeding strategies of whiteleg shrimp.
{"title":"Genetic Diversity and Genetic Structure among Four Selected Strains of Whiteleg Shrimp (Litopenaeus vannamei) Using SSR Markers","authors":"Zhe Zhang, Chengkuan Lu, Kebing Lin, Weiwei You, Zhangwu Yang","doi":"10.3390/fishes8110544","DOIUrl":"https://doi.org/10.3390/fishes8110544","url":null,"abstract":"The whiteleg shrimp (Litopenaeus vannamei) is one of the most economically important aquaculture species in China. Genetic diversity is crucial for maintaining the gene pool of farmed shrimp. In this study, the effects of artificial selection on the genetic structure of four whiteleg shrimp strains were evaluated using microsatellite sequences. The results showed that the observed heterozygosity (Ho), expected heterozygosity (He), and the polymorphism information content (PIC) of the four selected strains ranged from 0.446 to 0.574, 0.450 to 0.566, and 0.435 to 0.509, respectively. All the selected strains maintained a moderate level of genetic diversity. Most inbreeding coefficients (Fis) in the four strains were positive but not significantly different from zero, indicating a relatively low degree of inbreeding within each strain. However, He and PIC in line 5 showed a decreasing trend from the 2017 to the 2019 generations, and Fis in line 10 showed a significant increasing trend across generations, indicating that measures must be taken to maintain the level of genetic diversity for lines 5 and 10. UPGMA cluster trees showed that the four breeding lines had apparent genetic differences, which could provide a genetic basis for studying crossbreeding between selective lines and the utilization of heterosis. This study will be useful for population genetic research and the breeding strategies of whiteleg shrimp.","PeriodicalId":12405,"journal":{"name":"Fishes","volume":"3 4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135589734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Young-Bin Yu, Jae-Ho Choi, Ju-Hyeong Lee, A-Hyun Jo, Sung Won Han, Song-Hun Han, Hee Jae Choi, Cheol Young Choi, Ju-Chan Kang, EunYoung Min, Jun-Hwan Kim
Some aspects of traditional aquaculture have negative impacts on the aquatic environment, leading to pollution and disease outbreaks in farmed organisms. Biofloc technology (BFT) is a closed aquaculture system that utilizes specific microbial communities to remove ammonia emitted from aquaculture organisms or adds carbon to the aquaculture system to improve water quality. BFT has benefits, such as increasing production and improving water quality, and reducing disease spread and pollution, without the need for water exchange. However, there are disadvantages, such as rapid changes in water quality due to accumulation of dissolved nutrients and total suspended soils (TSS) and the requirement for expensive aeration equipment to maintain dissolved oxygen. BFT can be enhanced in value and efficiency by combining it with other aquaculture technologies, such as aquaponics and vertical aquaculture to overcome the disadvantages. The integration of biofloc with technologies from the fourth industrial revolution holds potential for further development, while aquaponics and vertical farming can eliminate geographical limitations and accelerate the urbanization of aquaculture. The integration of aquaponics and vertical aquaculture with BFT has potential for development, accelerating the urbanization of aquaculture and removing geographic limitations.
{"title":"Biofloc Application Using Aquaponics and Vertical Aquaculture Technology in Aquaculture: Review","authors":"Young-Bin Yu, Jae-Ho Choi, Ju-Hyeong Lee, A-Hyun Jo, Sung Won Han, Song-Hun Han, Hee Jae Choi, Cheol Young Choi, Ju-Chan Kang, EunYoung Min, Jun-Hwan Kim","doi":"10.3390/fishes8110543","DOIUrl":"https://doi.org/10.3390/fishes8110543","url":null,"abstract":"Some aspects of traditional aquaculture have negative impacts on the aquatic environment, leading to pollution and disease outbreaks in farmed organisms. Biofloc technology (BFT) is a closed aquaculture system that utilizes specific microbial communities to remove ammonia emitted from aquaculture organisms or adds carbon to the aquaculture system to improve water quality. BFT has benefits, such as increasing production and improving water quality, and reducing disease spread and pollution, without the need for water exchange. However, there are disadvantages, such as rapid changes in water quality due to accumulation of dissolved nutrients and total suspended soils (TSS) and the requirement for expensive aeration equipment to maintain dissolved oxygen. BFT can be enhanced in value and efficiency by combining it with other aquaculture technologies, such as aquaponics and vertical aquaculture to overcome the disadvantages. The integration of biofloc with technologies from the fourth industrial revolution holds potential for further development, while aquaponics and vertical farming can eliminate geographical limitations and accelerate the urbanization of aquaculture. The integration of aquaponics and vertical aquaculture with BFT has potential for development, accelerating the urbanization of aquaculture and removing geographic limitations.","PeriodicalId":12405,"journal":{"name":"Fishes","volume":"39 12","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135773650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mediha Yıldırım-Aksoy, Rashida Eljack, Janset Aksoy, Benjamin H. Beck
Frass–the by-product of the larva meal industry–was recently shown to be a sustainable ingredient for use in diets of several fish species. Additionally, because of its possible immunomodulatory activity and anti-microbial properties, frass may have great potential as an organic method of pathogen control in aquaculture. Five diets containing 0, 5, 10, 20 and 30% frass from black soldier fly larvae, Hermetia illucens, were fed to channel catfish (5.24 ± 0.04 g) in quadruplicate aquaria to apparent satiation twice daily. At the end of the 10-week feeding trial, blood samples were collected from all groups to measure hematological and immune parameters, and to determine the effects of dietary frass on resistance to Flavobacterium covae infection. Hematological parameters (red blood cell count, hemoglobin, and hematocrit)—but not white blood cell count—were improved with the inclusion of frass. Serum glucose levels were significantly lower in fish on the diet with frass than fish on the diet without frass. Fish fed the highest dietary levels of frass (30%) had a significantly higher serum cholesterol level than fish on the control diet. Serum complement activity was significantly higher in fish on diets containing frass at levels of 10% and 20%. No significant differences were observed in other measure serum components including albumin, alkaline phosphatase, alanine aminotransferase, amylase, calcium, phosphorus, potassium, total protein, globulin, thyroxine and lysozyme activity. Even though overall mortality was low (0–17%), fish on the diets containing frass at levels 20% or more showed significantly higher survival than that of control fish or fish on lower levels of dietary frass. The use of frass in the catfish diet may prove beneficial by improving hematological parameters, and select serum immune effectors, and the overall resistance of juvenile channel catfish against F. covae infection.
{"title":"Frass from Black Soldier Fly Larvae, Hermetia illucens, as a Possible Functional Dietary Ingredient in Channel Catfish Feed","authors":"Mediha Yıldırım-Aksoy, Rashida Eljack, Janset Aksoy, Benjamin H. Beck","doi":"10.3390/fishes8110542","DOIUrl":"https://doi.org/10.3390/fishes8110542","url":null,"abstract":"Frass–the by-product of the larva meal industry–was recently shown to be a sustainable ingredient for use in diets of several fish species. Additionally, because of its possible immunomodulatory activity and anti-microbial properties, frass may have great potential as an organic method of pathogen control in aquaculture. Five diets containing 0, 5, 10, 20 and 30% frass from black soldier fly larvae, Hermetia illucens, were fed to channel catfish (5.24 ± 0.04 g) in quadruplicate aquaria to apparent satiation twice daily. At the end of the 10-week feeding trial, blood samples were collected from all groups to measure hematological and immune parameters, and to determine the effects of dietary frass on resistance to Flavobacterium covae infection. Hematological parameters (red blood cell count, hemoglobin, and hematocrit)—but not white blood cell count—were improved with the inclusion of frass. Serum glucose levels were significantly lower in fish on the diet with frass than fish on the diet without frass. Fish fed the highest dietary levels of frass (30%) had a significantly higher serum cholesterol level than fish on the control diet. Serum complement activity was significantly higher in fish on diets containing frass at levels of 10% and 20%. No significant differences were observed in other measure serum components including albumin, alkaline phosphatase, alanine aminotransferase, amylase, calcium, phosphorus, potassium, total protein, globulin, thyroxine and lysozyme activity. Even though overall mortality was low (0–17%), fish on the diets containing frass at levels 20% or more showed significantly higher survival than that of control fish or fish on lower levels of dietary frass. The use of frass in the catfish diet may prove beneficial by improving hematological parameters, and select serum immune effectors, and the overall resistance of juvenile channel catfish against F. covae infection.","PeriodicalId":12405,"journal":{"name":"Fishes","volume":"7 6","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135934868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sara Madera-Santana, Carlos Rodríguez-García, Jairo Castro-Gutiérrez, Ángel Rafael Domínguez-Bustos, Remedios Cabrera-Castro
The brown comber (Serranus hepatus) is a small benthopelagic species with no commercial value, primarily caught by bottom trawls as a by-catch. In this work, we studied the feeding habits of this species. For this purpose, samples were obtained from the trawl fleet within the different editions of the ECOFISH project carried out between 2019 and 2022. A total of 1534 individuals were analyzed. In the diet analysis, various factors were considered, such as the season, the depth, and the time of day of the capture, as well as the size range of the individuals caught. For the feeding analysis, different indexes were calculated, such as the vacuity index (%Vi) and index of relative importance (%IRI). The size range of the specimens was between 3.2–16.3 cm, and the weight was between 1.02–39.73 g. Of the stomach content analyzed, 49.7% of the stomachs were found to be empty. The resources with the greatest importance in the diet of the brown comber were from the crustacean group, especially mysidaceans and decapods. There were differences in the diet according to season, depth, and size; however, there was no variation in diet by the time of day.
{"title":"Discarded but Not Dismissed: A Comprehensive Study of the Feeding Habits of the Brown Comber (Serranus hepatus, (Linneaus 1758)) in the Gulf of Cádiz (NE Atlantic)","authors":"Sara Madera-Santana, Carlos Rodríguez-García, Jairo Castro-Gutiérrez, Ángel Rafael Domínguez-Bustos, Remedios Cabrera-Castro","doi":"10.3390/fishes8110541","DOIUrl":"https://doi.org/10.3390/fishes8110541","url":null,"abstract":"The brown comber (Serranus hepatus) is a small benthopelagic species with no commercial value, primarily caught by bottom trawls as a by-catch. In this work, we studied the feeding habits of this species. For this purpose, samples were obtained from the trawl fleet within the different editions of the ECOFISH project carried out between 2019 and 2022. A total of 1534 individuals were analyzed. In the diet analysis, various factors were considered, such as the season, the depth, and the time of day of the capture, as well as the size range of the individuals caught. For the feeding analysis, different indexes were calculated, such as the vacuity index (%Vi) and index of relative importance (%IRI). The size range of the specimens was between 3.2–16.3 cm, and the weight was between 1.02–39.73 g. Of the stomach content analyzed, 49.7% of the stomachs were found to be empty. The resources with the greatest importance in the diet of the brown comber were from the crustacean group, especially mysidaceans and decapods. There were differences in the diet according to season, depth, and size; however, there was no variation in diet by the time of day.","PeriodicalId":12405,"journal":{"name":"Fishes","volume":"9 11","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135935079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yan Sun, Yi Huang, Ying Wang, Yanqun Wang, Guiying Hao, Changwei Jiang, Zhiqiu Huang
Nitrite toxicity poses a significant threat to aquatic organisms, including largemouth bass (LMB) and Micropterus salmoides. This study aimed to elucidate the role of bZIP transcription factors in mediating the molecular responses to nitrite stress in the LMB spleen. We identified 120 bZIP genes in the LMB genome using bioinformatics analysis and divided them into 11 subgroups based on phylogenetic relationships. Under nitrite stress, the bZIP_XI subgroup was upregulated, suggesting the activation of the stress response in the LMB spleen. Cellular pathway analysis revealed enrichment of pathways related to stress response, DNA repair, apoptosis, and autophagy. Co-expression network analysis highlighted bZIP_XI members such as msabZIP_49, msabZIP_12, msabZIP_39, and msabZIP_116 as potential key regulators. These transcription factors likely modulated the expression of stress-related genes like VCAM1, POLE3, and BMP1. Conserved binding motifs in the promoters of these genes may support regulation by bZIP_XI. Furthermore, bZIP_XI members correlated with immune cell infiltration in the spleen, potentially regulating immune-related genes like BCL2L1 and SELE. Homologs of bZIP_XI in other fish species exhibited similar expression patterns under stress. Overall, this study implicates the bZIP transcription factor family, notably the bZIP_XI subgroup, in orchestrating the molecular response of the LMB spleen to nitrite toxicity by regulating stress response pathways and immune function. These findings provide insights into nitrite stress adaptation in fish.
{"title":"The bZIP Transcription Factor Family Orchestrates the Molecular Response to Nitrite Stress in the Largemouth Bass Spleen","authors":"Yan Sun, Yi Huang, Ying Wang, Yanqun Wang, Guiying Hao, Changwei Jiang, Zhiqiu Huang","doi":"10.3390/fishes8110540","DOIUrl":"https://doi.org/10.3390/fishes8110540","url":null,"abstract":"Nitrite toxicity poses a significant threat to aquatic organisms, including largemouth bass (LMB) and Micropterus salmoides. This study aimed to elucidate the role of bZIP transcription factors in mediating the molecular responses to nitrite stress in the LMB spleen. We identified 120 bZIP genes in the LMB genome using bioinformatics analysis and divided them into 11 subgroups based on phylogenetic relationships. Under nitrite stress, the bZIP_XI subgroup was upregulated, suggesting the activation of the stress response in the LMB spleen. Cellular pathway analysis revealed enrichment of pathways related to stress response, DNA repair, apoptosis, and autophagy. Co-expression network analysis highlighted bZIP_XI members such as msabZIP_49, msabZIP_12, msabZIP_39, and msabZIP_116 as potential key regulators. These transcription factors likely modulated the expression of stress-related genes like VCAM1, POLE3, and BMP1. Conserved binding motifs in the promoters of these genes may support regulation by bZIP_XI. Furthermore, bZIP_XI members correlated with immune cell infiltration in the spleen, potentially regulating immune-related genes like BCL2L1 and SELE. Homologs of bZIP_XI in other fish species exhibited similar expression patterns under stress. Overall, this study implicates the bZIP transcription factor family, notably the bZIP_XI subgroup, in orchestrating the molecular response of the LMB spleen to nitrite toxicity by regulating stress response pathways and immune function. These findings provide insights into nitrite stress adaptation in fish.","PeriodicalId":12405,"journal":{"name":"Fishes","volume":"43 5","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135221203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ana R. Osowski, Matthew B. Jargowsky, Pearce T. Cooper, Sean P. Powers, J. Marcus Drymon
A better understanding of trophic interactions between hardhead catfish (Ariopsis felis) and gafftopsail catfish (Bagre marinus) is crucial for developing multi-species management strategies for the northern Gulf of Mexico (GOM). These two species are often aggregated in food web models; however, limited data are available to substantiate this approach. Therefore, the present study aimed to describe the dietary habits of hardhead catfish and gafftopsail catfish using analysis of stomach contents aided by DNA barcoding. Hardhead (n = 693) and gafftopsail (n = 655) catfish were sampled in the northern GOM from 2015–2019 using both fisheries-dependent and -independent techniques. The average percent number (%N), average percent mass (%M), prey specific number (%PN), prey specific mass (%PM), and prey-specific index of relative importance (%PSIRI) were computed to quantify prey species. The stomach content analysis identified distinct differences in diet between hardhead and gafftopsail catfish. Crustaceans were the most important prey for hardhead catfish, while gafftopsail catfish showed a significantly broader dietary breadth and were primarily piscivorous. Multivariate analyses indicated that the location of capture explained the greatest amount of diet variability for both species. These findings address fundamental knowledge gaps regarding the dietary habits of hardhead and gafftopsail catfish in northern GOM ecosystems.
{"title":"Dietary Habits of Hardhead (Ariopsis felis) and Gafftopsail (Bagre marinus) Catfish Revealed through DNA Barcoding of Stomach Contents","authors":"Ana R. Osowski, Matthew B. Jargowsky, Pearce T. Cooper, Sean P. Powers, J. Marcus Drymon","doi":"10.3390/fishes8110539","DOIUrl":"https://doi.org/10.3390/fishes8110539","url":null,"abstract":"A better understanding of trophic interactions between hardhead catfish (Ariopsis felis) and gafftopsail catfish (Bagre marinus) is crucial for developing multi-species management strategies for the northern Gulf of Mexico (GOM). These two species are often aggregated in food web models; however, limited data are available to substantiate this approach. Therefore, the present study aimed to describe the dietary habits of hardhead catfish and gafftopsail catfish using analysis of stomach contents aided by DNA barcoding. Hardhead (n = 693) and gafftopsail (n = 655) catfish were sampled in the northern GOM from 2015–2019 using both fisheries-dependent and -independent techniques. The average percent number (%N), average percent mass (%M), prey specific number (%PN), prey specific mass (%PM), and prey-specific index of relative importance (%PSIRI) were computed to quantify prey species. The stomach content analysis identified distinct differences in diet between hardhead and gafftopsail catfish. Crustaceans were the most important prey for hardhead catfish, while gafftopsail catfish showed a significantly broader dietary breadth and were primarily piscivorous. Multivariate analyses indicated that the location of capture explained the greatest amount of diet variability for both species. These findings address fundamental knowledge gaps regarding the dietary habits of hardhead and gafftopsail catfish in northern GOM ecosystems.","PeriodicalId":12405,"journal":{"name":"Fishes","volume":"29 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135808706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mojgan Zare Shahraki, Yazdan Keivany, Eisa Ebrahimi Dorche, Karen Blocksom, Andreas Bruder, Joseph Flotemersch, Doru Bănăduc
We assessed the distribution of alien fishes in the Karun River Basin, Iran. Fish were collected from 39 sites during the November–December 2018 low-flow period. In total, 39 fish species from nine orders and 14 families were documented. Among these, 10 species were alien to the basin (986 individuals; 15.7%). Four species were the most abundant alien species and primarily in impounded, downstream reaches. Redundancy analysis (RDA) was conducted to identify the extent of changes in alien fish assemblages with environmental parameters. RDA1 and RDA2 accounted for 36.24% and 25.33% of the variation of alien species, respectively. Altitude, depth, electrical conductivity, water temperature, turbidity, dissolved oxygen, and river width were the most significant parameters affecting alien species distributions. We present a dual-pathway cause-and-effect hypothesis proposing that alien fish species presence causes declines in the ecological status of native fish communities. We then explore how human-induced aquatic ecosystem degradation creates opportunities for alien species to invade new ecosystems, further impacting native fish communities. Our study contributes insight into the cause and effect of the presence of alien fish species in the Karun River Basin and emphasizes the urgency of conservation measures to protect this critically endangered watershed.
{"title":"Distribution and Expansion of Alien Fish Species in the Karun River Basin, Iran","authors":"Mojgan Zare Shahraki, Yazdan Keivany, Eisa Ebrahimi Dorche, Karen Blocksom, Andreas Bruder, Joseph Flotemersch, Doru Bănăduc","doi":"10.3390/fishes8110538","DOIUrl":"https://doi.org/10.3390/fishes8110538","url":null,"abstract":"We assessed the distribution of alien fishes in the Karun River Basin, Iran. Fish were collected from 39 sites during the November–December 2018 low-flow period. In total, 39 fish species from nine orders and 14 families were documented. Among these, 10 species were alien to the basin (986 individuals; 15.7%). Four species were the most abundant alien species and primarily in impounded, downstream reaches. Redundancy analysis (RDA) was conducted to identify the extent of changes in alien fish assemblages with environmental parameters. RDA1 and RDA2 accounted for 36.24% and 25.33% of the variation of alien species, respectively. Altitude, depth, electrical conductivity, water temperature, turbidity, dissolved oxygen, and river width were the most significant parameters affecting alien species distributions. We present a dual-pathway cause-and-effect hypothesis proposing that alien fish species presence causes declines in the ecological status of native fish communities. We then explore how human-induced aquatic ecosystem degradation creates opportunities for alien species to invade new ecosystems, further impacting native fish communities. Our study contributes insight into the cause and effect of the presence of alien fish species in the Karun River Basin and emphasizes the urgency of conservation measures to protect this critically endangered watershed.","PeriodicalId":12405,"journal":{"name":"Fishes","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135808567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aquatic products fulfill the protein needs of people and play an important role in food safety. And aquaculture is prized for its high productivity, sustainability and environmental friendliness. Considering the importance of aquaculture, the legal risks exposed during the aquaculture process deserve attention in order to prevent them from hindering the development of the aquaculture industry. Through online research, literature analysis and practical communication, it is shown that the current legal risks with commonalities include land use violations, lack of legal documents, failure to meet tailing water criteria, unquarantined fry and misuse of prohibited agricultural pharmaceuticals through online research, literature analysis and practical communication. By analyzing the reasons for the formation of legal risks and combining the experiences in sustainable development of three major aquaculture countries, which are Korea, Norway and Chile, this paper provides targeted preventive remedies and suggestions for aquaculture operators, administrative parties, legislators and other parties on legal risks. It includes promoting the improvement of the rule of law in multiple aspects, clarifying the positioning of the aquatic breeding certificates, improving and propagating the standards for wastewater discharge, increasing the self-sufficiency rate of aquatic fry and fingerlings, as well as making use of the synergy of soft law and hard law.
{"title":"Research on Legal Risk Identification, Causes and Remedies for Prevention and Control in China’s Aquaculture Industry","authors":"Chang Xu, Yang Liu, Zhaobin Pei","doi":"10.3390/fishes8110537","DOIUrl":"https://doi.org/10.3390/fishes8110537","url":null,"abstract":"Aquatic products fulfill the protein needs of people and play an important role in food safety. And aquaculture is prized for its high productivity, sustainability and environmental friendliness. Considering the importance of aquaculture, the legal risks exposed during the aquaculture process deserve attention in order to prevent them from hindering the development of the aquaculture industry. Through online research, literature analysis and practical communication, it is shown that the current legal risks with commonalities include land use violations, lack of legal documents, failure to meet tailing water criteria, unquarantined fry and misuse of prohibited agricultural pharmaceuticals through online research, literature analysis and practical communication. By analyzing the reasons for the formation of legal risks and combining the experiences in sustainable development of three major aquaculture countries, which are Korea, Norway and Chile, this paper provides targeted preventive remedies and suggestions for aquaculture operators, administrative parties, legislators and other parties on legal risks. It includes promoting the improvement of the rule of law in multiple aspects, clarifying the positioning of the aquatic breeding certificates, improving and propagating the standards for wastewater discharge, increasing the self-sufficiency rate of aquatic fry and fingerlings, as well as making use of the synergy of soft law and hard law.","PeriodicalId":12405,"journal":{"name":"Fishes","volume":"235 3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136157496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：