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A pilot study on the isolation and identification of histamine-producing bacteria from narrow barred Spanish mackerel and Japanese thread fin bream from Ukkadam fish market, Coimbatore, India 关于从印度哥印拜陀乌卡丹鱼类市场的窄条鲭鱼和日本线鳍鳊鱼中分离和鉴定产组胺细菌的试点研究
Pub Date : 2023-12-31 DOI: 10.55126/ijzab.2023.v08.i06.015
Vinisha Kartheesan, Mohammed Rafi L, Anju Alagiri, Velayutham Meiyalagan
Consumption of marine fishes with high histamine levels due to improper storing leads to cause scombroid or histamine fish poisoning. In spoiled fishes, bacteria begin to break down the histidine into histamine by decarboxylation activity. Previous studies reported that black muscle fishes usually produce large amounts of histamine-producing bacteria (HPB). In this study, two white muscle fishes such as Scomberomorus commerson (Narrow-barred Spanish mackerel) and Nemipterus japonicus (Japanese threadfin bream) were chosen to detect the presence of histamine-producing bacteria (HPB). The fish samples were purchased from Ukkadam fish market in Coimbatore and examined for the occurrence of HPB on Niven agar. The isolated HPB on media were analyzed for identification based on their morphological characteristics, primary test, and biochemical test. Finally, 8 colonies were confirmed as the cause for histamine production of both fishes and among them, 5 belong to the family Enterobacteriaceae and others belong to the family Aeromonadaceae, Morganellaceae, and Enterococcaceae. Our study showed that the histamine-producing bacteria highly reported in dark-muscle fishes were also isolated from the chosen white-muscle fishes. Hence the presence of HPB indicates the improper maintenance of fish in retail fish markets. This considerable incidence of histamine-producing bacteria which on proliferation under suitable conditions may contribute to toxic histamine accumulation in the flesh of fishes.
由于储存不当,食用组胺含量高的海鱼会导致鲭鱼或组胺鱼中毒。在变质的鱼类体内,细菌开始通过脱羧活动将组氨酸分解成组胺。以往的研究表明,黑肌鱼通常会产生大量的组胺产生菌(HPB)。本研究选择了两种白色肌肉鱼类,如窄带鲭(Scomberomorus commerson)和日本线鲷鱼(Nemipterus japonicus),来检测组胺产生细菌(HPB)的存在。鱼类样本购自哥印拜陀的 Ukkadam 鱼类市场,并在尼文琼脂上检测是否存在 HPB。根据培养基上分离出的 HPB 的形态特征、初级测试和生化测试,对其进行分析鉴定。最后,8 个菌落被确认为导致两种鱼类产生组胺的原因,其中 5 个菌落属于肠杆菌科,其他菌落属于气单胞菌科、摩根菌科和肠球菌科。我们的研究表明,在所选的白肌肉鱼类中也分离到了在黑肌肉鱼类中报道较多的产组胺细菌。因此,高致病性组胺细菌的存在表明零售鱼类市场对鱼类的维护不当。产生组胺的细菌在适当的条件下大量繁殖,可能会导致鱼肉中有毒组胺的积累。
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引用次数: 0
In vivo assessment of wound closure and biocompatibility of nanochitosan-polyvinylalcohol-glycerol films in experimental animal model 纳米壳聚糖-聚乙烯醇-甘油薄膜在实验动物模型中的伤口闭合和生物相容性的体内评估
Pub Date : 2023-12-31 DOI: 10.55126/ijzab.2023.v08.i06.011
Rosemol Jacob M., Amruth P, A. P, Stephy Rose K.V, A. R, Suseela Mathew
The need for innovative wound dressings has arisen due to injuries from second intention healing and their management is associated with high costs. Modern dressings not only protect wounds but also promote proliferation of cells and tissue regeneration. Patient compliance is crucial, ensuring that the dressing removal does not harm the newly regenerated tissue. In this study, nano-chitosan polyvinyl alcohol- glycerol (NCH-PVA-GLY) transparent wound dressing was developed that expedites tissue repair and can be removed through an external stimulus without disrupting the regenerated tissue. This dressing efficiently absorbs excess wound exudates while maintaining proper hydration. Evaluation in male Wistar rats with wounds revealed that the dressing, when applied for 16 days, resulted in minimal inflammation and denser connective tissue compared to wounds devoid of dressings. The dressing exhibited enhanced re-epithelization and angiogenesis, facilitating effective wound closure. The exposure of the resultant dressing, to the incised wound facilitated complete wound closure in 16 days, suggesting the effectiveness of the dressings to cure superficial wound.
由于第二意图愈合造成的伤害和高昂的管理费用,对创新型伤口敷料的需求应运而生。现代敷料不仅能保护伤口,还能促进细胞增殖和组织再生。患者的依从性至关重要,要确保敷料去除时不会伤害到新再生的组织。本研究开发了纳米壳聚糖聚乙烯醇-甘油(NCH-PVA-GLY)透明伤口敷料,可加快组织修复,并可通过外部刺激去除,而不会破坏再生组织。这种敷料能有效吸收多余的伤口渗出物,同时保持适当的水合作用。对雄性 Wistar 大鼠伤口的评估表明,与未使用敷料的伤口相比,使用这种敷料 16 天后,炎症极少,结缔组织更加致密。敷料还能增强伤口的再上皮和血管生成,从而促进伤口的有效闭合。将制成的敷料暴露在切开的伤口上,可促进伤口在 16 天内完全闭合,这表明敷料可有效治疗浅表伤口。
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引用次数: 0
Two new records of free-living marine nematodes (Nematoda: Enoplida: Ironidae and Monhysterida: Xyalidae) from India 印度自由生活的海洋线虫(线虫纲:铁线虫科和单细胞线虫科)的两项新记录
Pub Date : 2023-12-31 DOI: 10.55126/ijzab.2023.v08.i06.017
Ritika Datta, A. N. Rizvi, Raghunathan C
Two species of free-living marine nematodes were collected from the intertidal areas of East Coast of India namely, Conilia sinensis Chen and Guo, 2015 and Corononema parvum Nicholas and Stewart, 1995, both species are new records for the Indian fauna. Previously, the genus Conilia Gerlach, 1956 was known from Santos Beach, South Japan, East China Sea and Korea. The genus Corononema Nicholas and Stewart, 1995 was known only from Australia and Thailand and very recently reported from off the coast of Vietnam. Both the genera are being reported for the first time from India. The species are described and photomicrographed.
从印度东海岸潮间带采集到两种自由生活的海洋线虫,即Conilia sinensis Chen和Guo,2015年;Corononema parvum Nicholas和Stewart,1995年。此前,Conilia 属 Gerlach,1956 年已知产于桑托斯海滩、南日本、中国东海和韩国。尼古拉斯和斯图尔特,1995 年发现的 Corononema 属仅产于澳大利亚和泰国,最近在越南沿海也有报道。这两个属都是首次在印度发现。对其物种进行了描述和显微摄影。
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引用次数: 0
Trial for the propagation method in Flemingia spp., a shrub plant and a lac insect host in Thrissur, Kerala 喀拉拉邦 Thrissur 的灌木植物和漆树昆虫寄主 Flemingia 属的繁殖方法试验
Pub Date : 2023-12-31 DOI: 10.55126/ijzab.2023.v08.i06.012
S. Muthukumar, T.V. Sajeev, K. Thamilarasi, A. Mohanasundaram, V. Lohot
Due to the challenges posed by the poor availability and germination of Flemingia spp. for lac insect cultivation, the Kerala Forest Research Institute in Thrissur experimented with the air layering method and stem cutting (Vegetative Propagation) to enhance the number of seedlings. The layering method was successful for all three treatments, although it is observed variations in the length of the roots. The plant treated with coco peat exhibited longer roots and a greater number of roots, followed by the plants treated with only soil and vermiculite. The stem cutting method (vegetative propagation) was also successful for both plants, suggesting that both techniques can be used as alternatives for cultivating lac insect host plants instead of relying on seed germination.
由于用于漆虫栽培的佛焰苞属植物的供应和发芽率都很低,因此位于 Thrissur 的喀拉拉邦森林研究所尝试采用空气分层法和茎切割法(无性繁殖)来增加幼苗数量。虽然根的长度存在差异,但分层法在所有三种处理中都取得了成功。用椰子泥炭处理的植株根系较长,根系数量也较多,其次是仅用土壤和蛭石处理的植株。茎切割法(无性繁殖)对这两种植物也都取得了成功,这表明这两种技术都可用作培育漆虫寄主植物的替代方法,而不是依赖种子发芽。
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引用次数: 0
Anthelmintic efficacity of balanites Aegyptiaca (l.) Delile (Zygophyllaceae) almonds on three developmental stages of haemonchus contortus 杏仁(Zygophyllaceae)Aegyptiaca (l.) Delile 对线虫三个发育阶段的驱虫效果
Pub Date : 2023-12-31 DOI: 10.55126/ijzab.2023.v08.i06.009
Zabre Généviève, Traoré Aristide, Tianhoun Denté Fidèle, Segda Rimmibtiri, B. Balé, K. Adama, Belem Adrien Marie Gasto, Tamboura H. Hamidou
Gastrointestinal nematodes are a major constraint on small ruminant farming in Burkina Faso. To overcome this, rural farmers use the Balanites aegyptiaca plant to combat gastrointestinal parasites on their family farms. The aim of this study was therefore to investigate the in vitro anthelmintic efficacy of the aqueous extract of B. aegyptiaca almonds powder against the parasitic nematode Haemonchus contortus. To this end, in vitro tests were carried out to evaluate the biological activity of the aqueous extract using five increasing concentrations over the three life stages of H. contortus by performing the tests (i) inhibition of egg hatch, (ii) inhibition of larva development and (iii) mortality of adult female worms. The results showed 21.22-80.4 %, 47.80-88.57 % and 22.20-100 % of ovicide, larvicide and vermicide rates, respectively. The effects were dose and exposition time dependent. The median lethal inhibition concentrations (IC50) were estimated to be 2.20 and 0.84 mg/ mL for the inhibitionof egg and the inhibition of larva, respectively. It can be concluded that B. aegyptiaca almonds powder has anthelmintic activity against H. contortus. However, this activity needs to be verified in vivo to reassure farmers about its use in small ruminant raising.
肠道线虫是布基纳法索小型反刍动物养殖业的主要制约因素。为了克服这一问题,农村农民在家庭农场中使用埃及巴拉那植物(Balanites aegyptiaca)来防治胃肠道寄生虫。因此,本研究旨在调查埃及杏仁粉水提取物对寄生线虫Haemonchus contortus的体外驱虫效果。为此,我们进行了体外试验,通过(i) 抑制虫卵孵化、(ii) 抑制幼虫发育和(iii) 杀死雌性成虫等试验,评估了水提取物的生物活性。结果显示,杀卵率、杀幼虫率和杀害虫率分别为 21.22%-80.4%、47.80%-88.57% 和 22.20%-100%。杀虫效果与剂量和暴露时间有关。估计对卵和幼虫的中位致死抑制浓度(IC50)分别为 2.20 和 0.84 毫克/毫升。由此可以得出结论,埃及杏仁粉具有抗蠕虫活性。不过,这种活性还需要在体内进行验证,以便让农民放心地将其用于饲养小反刍动物。
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引用次数: 0
Preliminary investigations into the predator prey dynamics of Indian dinosaurs by agent-based modelling simulations 通过基于代理的建模模拟,对印度恐龙的捕食者和猎物动态进行初步研究
Pub Date : 2023-12-31 DOI: 10.55126/ijzab.2023.v08.i06.014
B. Sai Shankar
Advancements in computer simulations have facilitated the understanding of paleobiology and paleoecology of extinct animals even in cases of limited paleontological evidence. Two dinosaur species, Rajasaurus narmadensis(Wilson), and Isisaurus colberti (Jain & Bandyopadhyay) dominated the late Maastrichtian stage of upper Cretaceous Lameta formation in India, whose paleobiology is poorly understood. A preliminary approach to understand the predator prey dynamics of these two dinosaur species is presented in this study using Agent Based Modelling Simulations (ABMs) program Netlogo by modelling the agents under three scenarios of varying predator prey ratio (PPR) and analyzing the results. The results revealed that the scenario with the high PPR provided maximal predation success, faster and higher kill numbers and overall predation efficiency, similar to extant predators/ facultative scavengers. Further research including additional key factors in the simulation models are necessary for a better understanding of the paleoecology of these dinosaurs.
计算机模拟技术的进步促进了对已灭绝动物的古生物学和古生态学的了解,即使是在古生物学证据有限的情况下。Rajasaurus narmadensis(Wilson)和Isisaurus colberti(Jain & Bandyopadhyay)这两种恐龙在印度上白垩统Lameta地层的马斯特里赫特晚期占主导地位,而人们对它们的古生物学知之甚少。本研究利用基于代理的建模模拟(ABMs)程序 Netlogo,在三种不同的捕食猎物比例(PPR)情况下对代理进行建模,并对结果进行分析,从而初步了解了这两种恐龙的捕食猎物动态。结果表明,高捕食猎物比率的方案能提供最大的捕食成功率、更快和更高的捕杀数量以及总体捕食效率,与现存捕食者/兼性食腐动物类似。为了更好地了解这些恐龙的古生态,有必要在模拟模型中加入更多的关键因素,以开展进一步的研究。
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引用次数: 0
Evaluation of the proximate composition, minerals, and heavy metals of brown seaweeds: Sargassum wightii and Sargassum thunbergii collected from the Mandapam coastal regions, Tamil Nadu 评估棕色海藻的近似成分、矿物质和重金属:从泰米尔纳德邦曼达帕姆沿海地区采集的 wightii 马尾藻和 thunbergii 马尾藻
Pub Date : 2023-12-31 DOI: 10.55126/ijzab.2023.v08.i06.016
Preethy Treesa Paul, Jean Mary Joy, Fazil T.S, A. P, N. Chatterjee, Suseela Mathew
A vital renewable resource for the marine environment, seaweed naturally supplies nutrients and has several health benefits. The nutritional properties of seaweed compounds have yet to be well investigated; much research has been done on their chemical and biological makeup. Seaweeds provide an alternate supply of these essential nutrients, including protein, fibre, and necessary and non-essential trace elements. In the present study, two brown seaweeds-Sargassum wightii and Sargassum thunbergii,collected from the Mandapam coastal region of Tamil Nadu, were assessed for their proximate composition, minerals, and heavy metals.The findings on dry weight basis showed that carbohydrates (65.1–67.9%) predominated in the proximal analysis, followed by ash content (12.5–16.3%). On a dry weight basis, the critical trace elements found in seaweeds, such as copper (Cu), zinc (Zn), and iron (Fe), were between 8.14–12.41 ppm, 3.95–4.86 ppm, and 648.92-898.45 ppm, respectively. Both seaweeds have a very high calcium (Ca) concentration, ranging from (14,805.08-16,235.62) ppm. The seaweed species did not contain any measurable levels of dangerous heavy metals such as cadmium (Cd), beryllium (Be), lead (Pb), or mercury (Hg). The results demonstrated how seaweed or marine macroalgae may be helpful for essential nutrient supply.
海藻是海洋环境中一种重要的可再生资源,可天然提供营养物质,并对健康有多种益处。海藻化合物的营养特性还有待深入研究,但对其化学和生物构成的研究已经很多。海藻提供了这些必需营养素的替代供应,包括蛋白质、纤维、必需和非必需微量元素。本研究评估了从泰米尔纳德邦曼达帕姆沿海地区采集的两种褐色海藻--Sargassum wightii 和 Sargassum thunbergii 的近似成分、矿物质和重金属。按干重计算,在海藻中发现的关键微量元素,如铜(Cu)、锌(Zn)和铁(Fe),分别为 8.14-12.41 ppm、3.95-4.86 ppm 和 648.92-898.45 ppm。两种海藻的钙(Ca)浓度都很高,介于 (14,805.08-16,235.62) ppm 之间。这些海藻品种不含任何可测量到的危险重金属,如镉(Cd)、铍(Be)、铅(Pb)或汞(Hg)。研究结果表明,海藻或海洋大型藻类有助于提供必要的营养物质。
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引用次数: 0
Morphometric characters and meristic counts of two freshwater fishes of order Cypriniformes from song river Dehradun, Uttarakhand 北阿坎德邦宋河德拉敦两种鲤形目淡水鱼的形态特征和分体计数
Pub Date : 2023-11-30 DOI: 10.55126/ijzab.2023.v08.i06.001
Sanjay Paunikar, Fatima Kaneez
The study on morphometric and meristic characters of two species of fresh water fishes under order Cypriniformes were carried out in Ichthyology laboratory of Zoological Survey of India, Northern Regional Centre, Dehradun. During the present investigation, 2 families under Cyprinidae and Cobitidae fish species are molacarplet, Amblypharyngodonmola Hamilton (1822) and Lepidocephalichthys guntea (Hamilton, 1822) were identified. The 22 morphometric and 5 meristic counts were studied from Song River, Dehradun. The result showed that all the morphometric characters of two fishes are dependent on the total length. The analyzed data of morphometric dimensions of fish species would be beneficial for the policy makers or planners to develop the scientific strategies for their protection and conservation. The findings will also set the scientific principles for the management and sustainable development of the commercially essential freshwater food fishes.
对鲤形目淡水鱼类中两种鱼类的形态特征和分体特征的研究是在德拉敦印度动物学调查局北部区域中心鱼类学实验室进行的。在本次调查中,确定了鲤形目和鲭形目下的 2 个科的鱼类物种:鲻科鱼类 Amblypharyngodonmola Hamilton (1822) 和枪鱼科鱼类 Lepidocephalichthys guntea (Hamilton, 1822)。对德拉敦松江上的 22 种形态和 5 种特征进行了研究。结果表明,两种鱼类的所有形态特征都与总长度有关。鱼类物种形态测量的分析数据将有助于政策制定者或规划者制定保护和保存鱼类物种的科学战略。研究结果还将为商业上必需的淡水食用鱼的管理和可持续发展制定科学原则。
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引用次数: 0
Acaricidal effect of crude aqueous extract of Nicotiana tabacum along with turmeric and Annona leaf extract on cattle ticks 烟草粗水提取物以及姜黄和葵花叶提取物对牛蜱的杀螨效果
Pub Date : 2023-11-18 DOI: 10.55126/ijzab.2023.v08.i06.007
Kalaiselvi G, Jayanthi R, Ramya M, Anada Chitra M, Balakrishnan G, Jaisree S, Soundararajan C
The ectoparasites produces severe economic and production loss in farm animal’s through tick bites, sucking of blood, tick worry, tick toxicosis, tick paralysis and transmission of haemoprotozoan diseases. Ticks developed resistance against chemical acaricides and also the residues of chemical acaricide producing environmental pollution. The herbal based acaricide used as biocontrol agent for protecting environment particularly hilly area. As per indigenous technical knowledge records, the Nicotiana tabacum was a traditional control remedy for control of ectoparasites in India. In vitro acaricidal efficacy of crude aqueous leaf extract of Nicotiana tabacum along with neem and turmeric followed by Annona leaves on immature and adult stages of Rhipicephalus sp. was carried out at different concentrations of with different time interval. These extract combo had a significant effect on the mortality of nymphal and adult stages of ticks. The 70%, 80% and 90% mortality of Nymphal and adult stages of ticks at 24-36 hours post treatment with 40%, 50% 70% concentration of herbal extract in invitro assay. The mortality rates of ticks increased with the increasing the extract concentrations both in vivo and in vitro. Nicotiana tabacum causes paralysis of ticks and leads to detachment from the body of animal and concluded that Nicotiana tabacum along with turmeric and annona extract is effective biocontrol agent for controlling ectoparasites without any environmental pollution and harmful side effect in animal health.
体外寄生虫通过蜱虫叮咬、吸血、蜱虫忧虑、蜱虫中毒、蜱虫麻痹和传播血吸虫疾病,给农场动物造成严重的经济和生产损失。蜱虫对化学杀螨剂产生了抗药性,化学杀螨剂的残留物也造成了环境污染。以草药为基础的杀螨剂可用作生物控制剂来保护环境,尤其是丘陵地区。根据本土技术知识的记录,烟草是印度控制体外寄生虫的传统药方。研究人员用不同浓度、不同时间间隔的烟碱叶粗水提取物、印楝和姜黄以及艳阳叶对未成熟和成虫阶段的 Rhipicephalus sp.进行了体外杀螨试验。这些提取物组合对蜱的若虫期和成虫期的死亡率有明显的影响。用 40%、50% 和 70% 浓度的草药提取物进行体外试验,在处理后 24-36 小时内,蜱若虫和成虫的死亡率分别为 70%、80% 和 90%。蜱虫的死亡率随着体内和体外提取物浓度的增加而增加。结论是,烟草、姜黄和胭脂虫提取物是控制体外寄生虫的有效生物控制剂,不会对环境造成污染,也不会对动物健康产生有害的副作用。
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引用次数: 0
The leaf-aqueous extract of Pseudopanax arboreus(Pa)(Araliaceae) (L.F.Phillipson)repairs some reproductive indices impaired by Amitriptyline in male rats 阿米替林对雄性大鼠某些生殖指数的损伤修复作用
Pub Date : 2023-11-18 DOI: 10.55126/ijzab.2023.v08.i06.003
Egbe B. Besong, Nchegan Benjamin, Ateufack Gilbert, A. Kamanyi
Antidepressants have been associated with toxicity of the male reproductive system, altering testicular architecture, affecting hormonal profile and sperm characteristics, especially when administered for a long duration. Earlier, we reported the sex-enhancing potentials of Pseudpanax arboreus in normal male rats and its potentials to correct sexual deficiencies induced by amitriptyline in male rats. We undertook the present investigation to determine the curative properties of the leaf-aqueous extract of P. arboreus (Pa) on some indices of amitriptyline-induced male sexual impairments in rats. Sexually mature Albino male rats pre-exposed to10 mg/kg body weight of amitriptyline hydrochloride suspension for 56 days then allowed to self-recover for 11.5 days, were divided into 5 groups of 8 rats each and treated as follow: Group 1, pre-treated with amitriptyline, was allowed time for self-healing; Group 2 received 10ml/kg distilled water and served as negative control; Group 3 received 6mg/kg sildenafil citrateTM (Viagra) (positive control); while Groups 4 and 5 were administered 75 and 150mg/kg of the aqueous extract of P. arboreus, respectively. All extract-treated animals showed significant increase in relative weight of some sex and accessory organs, increased sperm motility and concentration as well as increased plasma concentrations of follicle stimulating hormone (FSH), luteinizing hormone (LH) and testosterone compared to the negative control animals. Also, the extract induced a significant improvement in the histo-architecture of the testes in sexually impaired animals, compared to the control group. In conclusion, the leaf-aqueous extract of Pseudopanax arboreus possesses curative properties on some male reproductive indices induced by amitriptyline.
抗抑郁药对雄性生殖系统具有毒性,会改变睾丸结构,影响激素水平和精子特征,尤其是在长期用药的情况下。早些时候,我们曾报道过假山在正常雄性大鼠中的性增强潜能,以及其纠正阿米替林诱导的雄性大鼠性缺陷的潜能。我们进行了本研究,以确定假山叶水提取物(Pa)对阿米替林诱导的雄性大鼠性缺陷的一些指标的治疗特性。将性成熟的白化雄性大鼠分为 5 组,每组 8 只,按以下方法处理:第 1 组,用盐酸阿米替林悬浮剂预处理 56 天,每公斤体重 10 毫克;第 2 组,用盐酸阿米替林悬浮剂预处理 56 天,每公斤体重 10 毫克;第 3 组,用盐酸阿米替林悬浮剂预处理 56 天,每公斤体重 10 毫克:第 1 组预先用阿米替林处理,让其自行恢复;第 2 组接受 10 毫升/千克蒸馏水,作为阴性对照;第 3 组接受 6 毫克/千克枸橼酸西地那非 TM(伟哥)(阳性对照);而第 4 组和第 5 组则分别服用 75 毫克/千克和 150 毫克/千克的熊果苷水提取物。与阴性对照组相比,所有经该提取物处理的动物的某些性器官和附属器官的相对重量均有明显增加,精子活力和浓度均有所提高,卵泡刺激素(FSH)、黄体生成素(LH)和睾酮的血浆浓度也有所提高。此外,与对照组相比,该提取物还能显著改善性功能受损动物睾丸的组织结构。总之,假山叶水提取物对阿米替林诱导的一些雄性生殖指数具有治疗作用。
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引用次数: 0
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International Journal of Zoology and Applied Biosciences
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