For targeted gene disruption in wild-type Neurospora crassa, 1000-bp of homologous sequences on either side of the cassette used for disruption is sufficient to give more than 10 % homologous recombination. We report here that varying the length of homology on each side seems to have different effects on the homologous recombination frequency. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol52/iss1/1
{"title":"An effect of homology length on gene disruption in Neurospora crassa","authors":"Y. Nakanishi, C. Ishii, H. Inoue","doi":"10.4148/1941-4765.1120","DOIUrl":"https://doi.org/10.4148/1941-4765.1120","url":null,"abstract":"For targeted gene disruption in wild-type Neurospora crassa, 1000-bp of homologous sequences on either side of the cassette used for disruption is sufficient to give more than 10 % homologous recombination. We report here that varying the length of homology on each side seems to have different effects on the homologous recombination frequency. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol52/iss1/1","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"132 1","pages":"5-6"},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75862516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The conventional approach for generating gene replacement constructs involves several sequence-specific cloning steps and is time-consuming. A ligation-PCR approach was developed to efficiently generate gene replacement constructs. Two vectors useful for this ligation-PCR approach and another vector suitable for improving the efficiency of knockout mutant screens were constructed. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol51/iss1/7
{"title":"A Ligation-PCR Approach for Generating Gene Replacement Constructs in Magnaporthe grisea","authors":"Xinhua Zhao, C. Xue, Yangseon Kim, Jin-Rong Xu","doi":"10.4148/1941-4765.1137","DOIUrl":"https://doi.org/10.4148/1941-4765.1137","url":null,"abstract":"The conventional approach for generating gene replacement constructs involves several sequence-specific cloning steps and is time-consuming. A ligation-PCR approach was developed to efficiently generate gene replacement constructs. Two vectors useful for this ligation-PCR approach and another vector suitable for improving the efficiency of knockout mutant screens were constructed. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol51/iss1/7","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"2016 1","pages":"17-18"},"PeriodicalIF":0.0,"publicationDate":"2004-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86552946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Strains obtained from the germination of 30-40 year old ascospores from seven stocks of the heterothallicAscobolus stercorarius furfuraceous complex were mated in all combinations. All st X st and fu X fu pairings were fertile (ascospores) whereas all st X fu pairings were sterile (no ascospores). Based on this reproductive isolation between st and fu strains I conclude that the complex consists of two species. The block that segregated the two species is probably some stage in the establishment of the dikaryotic phase in ascogenous hyphae. One hypothesis is that this block is at the stage of nuclear recognition. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol51/iss1/10
从7个异菌群的30-40岁子囊孢子萌发中获得的菌株在所有组合中都进行了交配。所有st X st和fu X fu配对都是可育的(子囊孢子)，而所有st X fu配对都是不育的(无子囊孢子)。根据st和fu菌株之间的生殖隔离，我得出结论，该复合体由两个物种组成。这两个物种分离的障碍可能是在球囊菌丝的双核阶段建立的某个阶段。一种假设是，该区块处于核识别阶段。本作品采用知识共享署名-相同方式共享4.0许可协议。这篇常规论文可在真菌遗传学报告:http://newprairiepress.org/fgr/vol51/iss1/10
{"title":"Reproductive Isolation: Evidence that Ascobolus stercorarius and Ascobolus furfuraceus are two species, not one","authors":"G. Bistis","doi":"10.4148/1941-4765.1140","DOIUrl":"https://doi.org/10.4148/1941-4765.1140","url":null,"abstract":"Strains obtained from the germination of 30-40 year old ascospores from seven stocks of the heterothallicAscobolus stercorarius furfuraceous complex were mated in all combinations. All st X st and fu X fu pairings were fertile (ascospores) whereas all st X fu pairings were sterile (no ascospores). Based on this reproductive isolation between st and fu strains I conclude that the complex consists of two species. The block that segregated the two species is probably some stage in the establishment of the dikaryotic phase in ascogenous hyphae. One hypothesis is that this block is at the stage of nuclear recognition. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol51/iss1/10","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"25 1","pages":"23-25"},"PeriodicalIF":0.0,"publicationDate":"2004-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88779141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Neurospora crassa cell-free extracts prepared from strains containing one or both functional Dicer genes, but not from a strain lacking functional Dicer genes, converts radiolabeled double-strand RNA (dsRNA) in an energy-dependent manner into short RNAs with an estimated size of ~25-nt (Catalanotto et al. 2004). A smaller nucleolytic digestion product was also produced in an energy-dependent manner from either dsRNA or single-stranded RNA. Here we obtained more precise sizes for these products by electrophoresis of samples on a long (40-cm) denaturing DNA sequencing gel (20% polyacrylamide/7M urea). Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol51/iss1/9
从含有一个或两个功能Dicer基因的菌株制备的粗神经孢子虫无细胞提取物，而不是从缺乏功能Dicer基因的菌株制备的提取物，以能量依赖的方式将放射性标记的双链RNA (dsRNA)转化为估计大小为~25 nt的短RNA (Catalanotto et al. 2004)。从dsRNA或单链RNA中也以能量依赖的方式产生较小的溶核消化产物。在这里，我们通过在长(40厘米)变性DNA测序凝胶(20%聚丙烯酰胺/7M尿素)上对样品进行电泳，获得了这些产品更精确的尺寸。本作品采用知识共享署名-相同方式共享4.0许可协议。这篇常规论文可在真菌遗传学报告:http://newprairiepress.org/fgr/vol51/iss1/9
{"title":"A precise size-estimate for the small RNA products arising from Neurospora crassa Dicer activity","authors":"P. Refalo, M. Sachs","doi":"10.4148/1941-4765.1139","DOIUrl":"https://doi.org/10.4148/1941-4765.1139","url":null,"abstract":"Neurospora crassa cell-free extracts prepared from strains containing one or both functional Dicer genes, but not from a strain lacking functional Dicer genes, converts radiolabeled double-strand RNA (dsRNA) in an energy-dependent manner into short RNAs with an estimated size of ~25-nt (Catalanotto et al. 2004). A smaller nucleolytic digestion product was also produced in an energy-dependent manner from either dsRNA or single-stranded RNA. Here we obtained more precise sizes for these products by electrophoresis of samples on a long (40-cm) denaturing DNA sequencing gel (20% polyacrylamide/7M urea). Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol51/iss1/9","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"10 1","pages":"21-22"},"PeriodicalIF":0.0,"publicationDate":"2004-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88514183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Although the Neurospora crassa circadian clock has been studied for forty years, population studies of natural accessions have been limited by technical difficulties associated with the conventional race tube assay (CRTA) that is used to measure asexual development (conidiation). Due to the buildup of CO2 in the CRTA that represses banding, a mutant strain band (bd) has been utilized for increased visualization of the banding phenotype. In order to study the circadian clock in natural accessions of Neurospora multiple techniques have been explored. One such technique, the rubidium chloride-supplemented race tube assay (RRTA) has been used successfully. Here we present a new technique, the Inverted Race Tube Assay (IRTA) that is a simple modification of the CRTA. We analyzed 5 natural accessions of Neurospora using CRTA, IRTA and RRTA and discuss the advantages of the IRTA in natural variation studies in Neurospora. This regular paper is available in Fungal Genetics Reports: https://newprairiepress.org/fgr/vol51/iss1/5 12 Fungal Genetics Newsletter Inverted Race Tube Assay for Circadian Clock Studies of the Neurospora Accessions. Sohyun Park, Kwangwon Lee. Department of Plant Pathology, Cornell University, Ithaca NY 14850 Fungal Genet. Newsl. 51:12-14 Although the Neurospora crassa circadian clock has been studied for forty years, population studies of natural accessions have been limited by technical difficulties associated with the conventional race tube assay (CRTA) that is used to measure asexual development (conidiation). Due to the buildup of CO2 in the CRTA that represses banding, a mutant strain band (bd) has been utilized for increased visualization of the banding phenotype. In order to study the circadian clock in natural accessions of Neurospora multiple techniques have been explored. One such technique, the rubidium chloride-supplemented race tube assay (RRTA) has been used successfully. Here we present a new technique, the Inverted Race Tube Assay (IRTA) that is a simple modification of the CRTA. We analyzed 5 natural accessions of Neurospora using CRTA, IRTA and RRTA and discuss the advantages of the IRTA in natural variation studies in Neurospora. N. crassa has proved to be a successful model for studying the molecular bases of circadian clocks. Significant insights into the mechanism of the clock have been obtained in N. crassa including a) transcriptional/translational feedback loops in the clock that opened up the conceptual foundation for our understanding of the biological clock (Aronson et al.1994); b) the resetting mechanisms by light and temperature (Liu et al. 1998; Crosthwaite et al. 1995); c) circadian gating of cellular activities (Heintzen et al. 2001); and d) the involvement of anti-sense RNA in the circadian regulatory circuit (Kramer et al. 2003). A critical step in studying the circadian clock is the ability to easily observe and measure circadian regulated behavior. The rhythmic behavior that has been most extensiv
除了将新鲜空气吹入管中外，还建议将天然材料与bd突变体杂交，或采用氯化铷补充管试验(RRTA) (M organ and Feldman 1998)作为监测神经孢子菌材料条带的方法。然而，这些解决方案都产生了额外的问题。首先，与bd菌株杂交是不可取的，因为它不能保持材料之间基因组结构的异质性。其次，RRTA引入了另一个变量;氯化铷的作用方式没有被描述，增加了一个未知的环境因素，使定量研究进一步复杂化(Morgan and Feldman 1998)。考虑到所有这些技术问题，我们开发了一种改进的race tube assay，即倒置race tube assay (IRTA)(图1)。我们推断，如果真的只有被困在race tube底部的CO2阻止了有节奏的无性发育[8]，那么只需将race tube倒置即可去除重CO2。将25 ml培养基(1X Vogel, l -精氨酸0.17%，d -葡萄糖0.1%，琼脂1.5%)倒入试管中，高压灭菌(121 /C) 20分钟。随着介质冷却，介质凝固;由于比赛管内表面的冷凝水，当比赛管倒转时，固体介质会沉淀在比赛管的底部(以前是顶部)。然后将比赛管放在一个平坦的表面过夜，直到比赛管内多余的水被干燥。1 Park and Lee:神经生物钟研究的倒置种族管试验，新草原出版社，2017年出版
{"title":"Inverted Race Tube Assay for Circadian Clock Studies of the Neurospora Accessions","authors":"Sohyun Park, Kwangwon Lee","doi":"10.4148/1941-4765.1135","DOIUrl":"https://doi.org/10.4148/1941-4765.1135","url":null,"abstract":"Although the Neurospora crassa circadian clock has been studied for forty years, population studies of natural accessions have been limited by technical difficulties associated with the conventional race tube assay (CRTA) that is used to measure asexual development (conidiation). Due to the buildup of CO2 in the CRTA that represses banding, a mutant strain band (bd) has been utilized for increased visualization of the banding phenotype. In order to study the circadian clock in natural accessions of Neurospora multiple techniques have been explored. One such technique, the rubidium chloride-supplemented race tube assay (RRTA) has been used successfully. Here we present a new technique, the Inverted Race Tube Assay (IRTA) that is a simple modification of the CRTA. We analyzed 5 natural accessions of Neurospora using CRTA, IRTA and RRTA and discuss the advantages of the IRTA in natural variation studies in Neurospora. This regular paper is available in Fungal Genetics Reports: https://newprairiepress.org/fgr/vol51/iss1/5 12 Fungal Genetics Newsletter Inverted Race Tube Assay for Circadian Clock Studies of the Neurospora Accessions. Sohyun Park, Kwangwon Lee. Department of Plant Pathology, Cornell University, Ithaca NY 14850 Fungal Genet. Newsl. 51:12-14 Although the Neurospora crassa circadian clock has been studied for forty years, population studies of natural accessions have been limited by technical difficulties associated with the conventional race tube assay (CRTA) that is used to measure asexual development (conidiation). Due to the buildup of CO2 in the CRTA that represses banding, a mutant strain band (bd) has been utilized for increased visualization of the banding phenotype. In order to study the circadian clock in natural accessions of Neurospora multiple techniques have been explored. One such technique, the rubidium chloride-supplemented race tube assay (RRTA) has been used successfully. Here we present a new technique, the Inverted Race Tube Assay (IRTA) that is a simple modification of the CRTA. We analyzed 5 natural accessions of Neurospora using CRTA, IRTA and RRTA and discuss the advantages of the IRTA in natural variation studies in Neurospora. N. crassa has proved to be a successful model for studying the molecular bases of circadian clocks. Significant insights into the mechanism of the clock have been obtained in N. crassa including a) transcriptional/translational feedback loops in the clock that opened up the conceptual foundation for our understanding of the biological clock (Aronson et al.1994); b) the resetting mechanisms by light and temperature (Liu et al. 1998; Crosthwaite et al. 1995); c) circadian gating of cellular activities (Heintzen et al. 2001); and d) the involvement of anti-sense RNA in the circadian regulatory circuit (Kramer et al. 2003). A critical step in studying the circadian clock is the ability to easily observe and measure circadian regulated behavior. The rhythmic behavior that has been most extensiv","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"12 1","pages":"12-14"},"PeriodicalIF":0.0,"publicationDate":"2004-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85666415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This medium was designed to circumvent some problems that arise in the use of Medium N (Vogel 1964 Am. Naturalist 98:435-446). These are, among others, the presence of high levels of citrate, a chelator which leaves the concentration of calcium and trace elements uncertain; the use of ammonium nitrate, which leaves the actual source of nitrogen ambiguous; the use of MgSO4, which does not allow the experimenter to vary the concentration of magnesium and sulfur independently; the high activity coefficient for the pKa values of citrate, which makes the pH unnecessarily sensitive to ionic strength; the use of sucrose, which leaves uncertain the nature and relative amounts of the hexose(s) being used at any particular moment; the need to use chloroform as a preservative, which results in the gradual depletion of the aqueous phase of complexes of trace elements. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol51/iss1/8
{"title":"Bird Medium: an alternative to Vogel Medium","authors":"R. L. Metzenberg","doi":"10.4148/1941-4765.1138","DOIUrl":"https://doi.org/10.4148/1941-4765.1138","url":null,"abstract":"This medium was designed to circumvent some problems that arise in the use of Medium N (Vogel 1964 Am. Naturalist 98:435-446). These are, among others, the presence of high levels of citrate, a chelator which leaves the concentration of calcium and trace elements uncertain; the use of ammonium nitrate, which leaves the actual source of nitrogen ambiguous; the use of MgSO4, which does not allow the experimenter to vary the concentration of magnesium and sulfur independently; the high activity coefficient for the pKa values of citrate, which makes the pH unnecessarily sensitive to ionic strength; the use of sucrose, which leaves uncertain the nature and relative amounts of the hexose(s) being used at any particular moment; the need to use chloroform as a preservative, which results in the gradual depletion of the aqueous phase of complexes of trace elements. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol51/iss1/8","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"4 1","pages":"19-20"},"PeriodicalIF":0.0,"publicationDate":"2004-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75424604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The process of mating, fertilization, fruiting, meiosis and spore formation is regulated by two kinds of genetic factors residing at the A and B mating-type loci, earlier called incompatibility factors A and B. Over the eight decades since Kniep's discovery, revelations about the genetic, biochemical and molecular underpinnings of this bizarre system have made an exciting story (see list of selected references, below). While other interesting aspects of Schizophyllum have been explored, notably the hydrophobins of Wessels and associates (reviewed in W essels, 2000), a principal focus over the years has been on mating compatibility and sexual development. Although Schizophyllum commune 's main role in nature is to recycle carbon by breaking down celluose and xylans in fallen wood (Clarke and Yaguchi, 1986; Bray and Clarke, 1995), it has been documented occasionally as a pathogen in fruit orchards (Latham, 1970; Oprea, et al, 1995) and also in immunologically compromised humans (Buzina et al, 2001).
交配、受精、结果、减数分裂和孢子形成的过程是由位于A和B交配型位点的两种遗传因子调控的，这两种遗传因子以前被称为不亲和因子A和B。在Kniep发现后的80年里，关于这一奇异系统的遗传、生化和分子基础的揭示创造了一个令人兴奋的故事(见下面的精选参考文献列表)。虽然裂叶植物的其他有趣的方面已经被探索过，特别是Wessels及其同伴的疏水蛋白(在Wessels, 2000中进行了综述)，但多年来的主要焦点一直是交配相容性和性发育。尽管裂叶菌群落在自然界中的主要作用是通过分解落木中的纤维素和木质素来回收碳(Clarke and Yaguchi, 1986;Bray和Clarke, 1995)，它偶尔被记录为果园中的病原体(Latham, 1970;Oprea等人，1995年)以及免疫功能受损的人(Buzina等人，2001年)。
{"title":"Why Study Schizophyllum","authors":"C. Raper, T. Fowler","doi":"10.4148/1941-4765.1142","DOIUrl":"https://doi.org/10.4148/1941-4765.1142","url":null,"abstract":"The process of mating, fertilization, fruiting, meiosis and spore formation is regulated by two kinds of genetic factors residing at the A and B mating-type loci, earlier called incompatibility factors A and B. Over the eight decades since Kniep's discovery, revelations about the genetic, biochemical and molecular underpinnings of this bizarre system have made an exciting story (see list of selected references, below). While other interesting aspects of Schizophyllum have been explored, notably the hydrophobins of Wessels and associates (reviewed in W essels, 2000), a principal focus over the years has been on mating compatibility and sexual development. Although Schizophyllum commune 's main role in nature is to recycle carbon by breaking down celluose and xylans in fallen wood (Clarke and Yaguchi, 1986; Bray and Clarke, 1995), it has been documented occasionally as a pathogen in fruit orchards (Latham, 1970; Oprea, et al, 1995) and also in immunologically compromised humans (Buzina et al, 2001).","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"63 1.2 1","pages":"30-36"},"PeriodicalIF":0.0,"publicationDate":"2004-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91165168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A multiple-site molecular dimorphism for the gene for phospholipase C has no known evolutionary basis, yet reveals that Oak Ridge wild types cannot have originated as described in the current best pedigree. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol51/iss1/4
{"title":"Another inconsistency in the pedigree of the Oak Ridge wild types of Neurospora crassa","authors":"O. Gavric, A. Griffiths","doi":"10.4148/1941-4765.1134","DOIUrl":"https://doi.org/10.4148/1941-4765.1134","url":null,"abstract":"A multiple-site molecular dimorphism for the gene for phospholipase C has no known evolutionary basis, yet reveals that Oak Ridge wild types cannot have originated as described in the current best pedigree. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol51/iss1/4","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"62 1","pages":"9-11"},"PeriodicalIF":0.0,"publicationDate":"2004-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83693587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The highly inbred Neurospora crassa strains 74-OR23-1VA (FGSC 2489) and 74-ORS-6a (FGSC 4200) are recommended for use as standard wild types. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol51/iss1/3
{"title":"Wild type Neurospora crassa strains preferred for use as standards","authors":"D. D. Perkins","doi":"10.4148/1941-4765.1133","DOIUrl":"https://doi.org/10.4148/1941-4765.1133","url":null,"abstract":"The highly inbred Neurospora crassa strains 74-OR23-1VA (FGSC 2489) and 74-ORS-6a (FGSC 4200) are recommended for use as standard wild types. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol51/iss1/3","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"25 1","pages":"7-8"},"PeriodicalIF":0.0,"publicationDate":"2004-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78444090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eighteen PCR-based markers are described for use in mapping mutations in Oak Ridge background strains of Neurospora crassa. These markers are located on each of the seven linkage groups and in the mitochondrial genome to enable course-scale linkage mapping. Following mapping to a linkage group, additional markers can be developed in the co-segregating region for fine-scale mapping of mutations. As with the N. crassa RFLP map (Metzenberg and Grotelueschen, 1993; Nelson and Perkins, 2000), the addition of PCR-based markers by members of the Neurospora community will enhance this marker set for mapping purposes. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol51/iss1/11 26 Fungal Genetics Newsletter PCR-based markers for genetic mapping in Neurospora crassa . Moshi Kotierk and Myron L. Smith, Carleton University, Ottawa-Carleton Institute of Biology, 1125 Colonel By Drive, Ottawa, Ontario, K1S 5B6, Canada Fungal Genetics Newsletter 51:26-26 Eighteen PCR-based markers are described for use in mapping mutations in Oak Ridge background strains of Neurospora crassa . These markers are located on each of the seven linkage groups and in the mitochondrial genome to enable course-scale linkage mapping. Following mapping to a linkage group, additional markers can be developed in the co-segregating region for fine-scale mapping of mutations. As with the N. crassa RFLP map (Metzenberg and Grotelueschen, 1993; Nelson and Perkins, 2000), the addition of PCR-based markers by members of the Neurospora community will enhance this marker set for mapping purposes. Map-based or positional cloning is necessary to locate, identify and characterize genes that are associated with spontaneous or induced mutations. Methods available for locating mutations in Neurospora crassa involve co-segregation analysis using phenotypic (e.g. auxotrophic) markers. For example, multiply marked centromere tester strains of N. crassa are available for this purpose through the Fungal Genetics Stock Center (e.g. Perkins, 1972; Metzenberg et al., 1984). Often, however, co-segregation analysis with a set of phenotypic markers will not provide adequate resolution for the fine-scale linkage mapping necessary to clone a trait of interest and may require subsequent crosses to achieve further resolution. In this note, PCR-based markers are described for locating mutations generated in Oak Ridge background strains. The method takes advantage of abundant sequence differences between Oak Ridge and M auriceville genetic backgrounds, and the recently completed N. crassa genome sequence of the Oak Ridge standard strain 74-OR23-1VA (Galagan et al., 2003). The PCR-based markers are distributed throughout the seven N. crassa linkage groups and the mitochondrial DNA (Figure 1). Mapping of a mutation involves four steps. 1) Crossing an Oak Ridge-background
这18个标记和其他由neurosporac社区添加的标记将为感兴趣的性状的定位克隆提供有价值的资源。致谢本研究的资金由加拿大自然科学与工程研究委员会的发现基金提供给MLS，由新信用第一民族的密西沙加斯提供给M . K. John Vierula博士提供了nnt -1标记的引物。新草原出版社2017年出版
{"title":"PCR-based markers for genetic mapping in Neurospora crassa.","authors":"Moshi Kotierk, Myron L Smith","doi":"10.4148/1941-4765.1141","DOIUrl":"https://doi.org/10.4148/1941-4765.1141","url":null,"abstract":"Eighteen PCR-based markers are described for use in mapping mutations in Oak Ridge background strains of Neurospora crassa. These markers are located on each of the seven linkage groups and in the mitochondrial genome to enable course-scale linkage mapping. Following mapping to a linkage group, additional markers can be developed in the co-segregating region for fine-scale mapping of mutations. As with the N. crassa RFLP map (Metzenberg and Grotelueschen, 1993; Nelson and Perkins, 2000), the addition of PCR-based markers by members of the Neurospora community will enhance this marker set for mapping purposes. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol51/iss1/11 26 Fungal Genetics Newsletter PCR-based markers for genetic mapping in Neurospora crassa . Moshi Kotierk and Myron L. Smith, Carleton University, Ottawa-Carleton Institute of Biology, 1125 Colonel By Drive, Ottawa, Ontario, K1S 5B6, Canada Fungal Genetics Newsletter 51:26-26 Eighteen PCR-based markers are described for use in mapping mutations in Oak Ridge background strains of Neurospora crassa . These markers are located on each of the seven linkage groups and in the mitochondrial genome to enable course-scale linkage mapping. Following mapping to a linkage group, additional markers can be developed in the co-segregating region for fine-scale mapping of mutations. As with the N. crassa RFLP map (Metzenberg and Grotelueschen, 1993; Nelson and Perkins, 2000), the addition of PCR-based markers by members of the Neurospora community will enhance this marker set for mapping purposes. Map-based or positional cloning is necessary to locate, identify and characterize genes that are associated with spontaneous or induced mutations. Methods available for locating mutations in Neurospora crassa involve co-segregation analysis using phenotypic (e.g. auxotrophic) markers. For example, multiply marked centromere tester strains of N. crassa are available for this purpose through the Fungal Genetics Stock Center (e.g. Perkins, 1972; Metzenberg et al., 1984). Often, however, co-segregation analysis with a set of phenotypic markers will not provide adequate resolution for the fine-scale linkage mapping necessary to clone a trait of interest and may require subsequent crosses to achieve further resolution. In this note, PCR-based markers are described for locating mutations generated in Oak Ridge background strains. The method takes advantage of abundant sequence differences between Oak Ridge and M auriceville genetic backgrounds, and the recently completed N. crassa genome sequence of the Oak Ridge standard strain 74-OR23-1VA (Galagan et al., 2003). The PCR-based markers are distributed throughout the seven N. crassa linkage groups and the mitochondrial DNA (Figure 1). Mapping of a mutation involves four steps. 1) Crossing an Oak Ridge-background ","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"386 1","pages":"26-29"},"PeriodicalIF":0.0,"publicationDate":"2004-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77123904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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