Fungal Genetics Reports最新文献

{"title":"Schizophyllum commune Bibliography, May 2004","authors":"C. Raper, T. Fowler","doi":"10.4148/1941-4765.1143","DOIUrl":"https://doi.org/10.4148/1941-4765.1143","url":null,"abstract":"Schizophyllum commune Bibliography, May 2004 Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This bibliography is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol51/iss1/13 Schizophyllum commune Bibliography, May 2004 Carlene A. Raper and Thomas J. Fowler Department of Microbiology and Molecular Genetics Stafford Hall, University of Vermont Burlington VT, 05405 Ahmad, S. S. (1969).Studies on Hyphal Fusions in the Wood-Rotting Fungus, Schizophyllum commune: State University of New York at Buffalo. Ahmad, S. S. & Miles, P. G. (1970). Hyphal Fusions in the Wood-Rotting Fungus Schizophyllum commune. I. The Effects of Incompatibility Factors. Genet Res 15, 19-28. Aitken, W. B. (1970).Schizophyllum commune Basidiospore Germination: Biochemical and Physiological Alterations: Indiana University. Aitken, W. B. & Niederpruem, D. J. (1972). Isotopic Studies of Carbohydrate Metabolism During Basidiospore Germination in Schizophyllum commune. I. Uptake of Radioactive Glucose and Sugar Alcohols. Arch Mikrobiol. Aitken, W. B. & Niederpruem, D. J. (1973). Isotopic Studies of Carbohydrate Metabolism During Basidiospore Germination in Schizophyllum commune. Ii. Changes in Specifically Labeled Glucose and Sugar Alcohol Utilization. Arch Mikrobiol. Alic, M., Clark, E. K., Kornegay, J. R. & Gold, M. H. (1990). Transformation of Phanerochaete chrysosporium and Neurospora crassa with adenine biosynthetic genes from Schizophyllum commune. Current genetics 17, 305-311. Amitani, R., Nishimura, K., Mimi, A., Kobayashi, H., Nawada, R., Murayama, T., Taguchi, H. & Kuze, F. (1996). Bronchial Mucoid Impaction Due to the Monokaryotic Mycelium of Schizophyllum commune. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 22, 3. Anderson, M. R. & Deppe, C. S. (1976). Control of Fungal Development. I. The Effects of Two Regulatory Genes on Growth in Schizophyllum commune. Dev Biol 53, 21-29. Anderson, M. R. & Deppe, C. S. (1977). Selection for Conditional Lethals: A General Negative Selective System for [the Filamentous Fungus] Schizophyllum commune. Genet Res 29, 93-96. Asada, Y., Yue, C. L., Wu, J., Shen, G. P., Novotny, C. P. & Ullrich, R. C. (1997). Schizophyllum commune A alpha mating-type proteins, Y and Z, form complexes in all combinations in vitro. Genetics 147, 117-123. Asbirk, S. (1976). Udbredelsen Af Svampen Klovblad, Schizophyllum commune, I Danmark. Flora Fauna 82, 83-84. Asgeirsdottir, S. A., Schuren, F. H. J. & Wessels, J. G. H. (1994). Assignment of genes to pulsefield separated chromosomes of Schizophyllum commune. Mycological research 98, 689-693. Published by New Prairie Press, 2017 Asgeirsdottir, S. A., Wetter, M. A. v. & Wessels, J. G. H. (1995). Differential expression of genes under control of the mating-type genes in the secondary mycelium of Schizophyllum commune. Microbiology 141, 8. Bartholomew, K., Dos Santos, G., Dumonc","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"3 1","pages":"37-66"},"PeriodicalIF":0.0,"publicationDate":"2004-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79018235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Why are Neurospora crassa crosses that are homozygous for a large duplication barren","authors":"A. Bhat, D. P. Kasbekar","doi":"10.4148/1941-4765.1136","DOIUrl":"https://doi.org/10.4148/1941-4765.1136","url":null,"abstract":"Crosses homozygous for the duplication Dp(AR17) are barren regardless of RIP. Sad-1, a semi-dominant suppressor of meiotic silencing, suppresses the barrenness of duplication-heterozygous but not of duplicationhomozygous crosses. Could it be that in the context of the homozygous cross the sad-1+ allele is not detected as being unpaired, and consequently, Sad-1 fails to suppress meiotic silencing? Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol51/iss1/6","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"5 1","pages":"15-16"},"PeriodicalIF":0.0,"publicationDate":"2004-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74186944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"pZHK2, a bi-functional transformation vector, suitable for two step gene targeting","authors":"U. Kück, S. Pöggeler","doi":"10.4148/1941-4765.1132","DOIUrl":"https://doi.org/10.4148/1941-4765.1132","url":null,"abstract":"Homologous recombination is a prerequisite for the generation of knock out strains by means of DNA-mediated transformation. In filamentous fungi however, the frequency of ectopic integration events is rather high and the actual efficiency of homologous recombination depends upon the length of homologous DNA flanking the transformation marker. Recently, d'Enfert and coworkers (Chaveroche et al., 2000) presented a two-step technology for the integration of a bi-functional zeocin-pyrG cassette into a target sequence of interest using an Escherichia coli strain expressing the phage lambda Red functions. In the resulting recombinant cosmids, the selection marker is flanked by fungal DNA sequences longer than 1 kb, which can be used to transform appropriate fungal recipient strains. For selection of fungal transformants, those workers used the A. nidulans pyrG gene encoding orotidine-5'monophosphate decarboxylase, which confers prototrophy in appropriate uridine/uracil auxotrophic recipient strains. Here, we describe the novel bi-functional transformation vector pZHK2, which carries in addition to the zeocin resistance gene the hygromycin B phosphotransferase gene often used as a dominant selectable marker gene in fungal recipient strains. The applicability of the vector is demonstrated by generating a ura3 knock out strain from Sordaria macrospora showing auxotrophy.","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"363 Pt 3 1","pages":"4-6"},"PeriodicalIF":0.0,"publicationDate":"2004-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79651234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Podospora anserina bibliography n° 10 - Additions","authors":"R. Debuchy","doi":"10.4148/1941-4765.1161","DOIUrl":"https://doi.org/10.4148/1941-4765.1161","url":null,"abstract":"Podospora anserina is a coprophilous fungus growing on herbivore dung. It is a pseudohomothallic species in which ascus development results, as in Neurospora tetrasperma but through a different process, in the formation of four large ascospores containing nuclei of both mating types. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This special paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol50/iss1/15","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"1 1","pages":"27-36"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80031911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Foreword to FGN 50, Online Edition","authors":"M. Sachs","doi":"10.4148/1941-4765.1147","DOIUrl":"https://doi.org/10.4148/1941-4765.1147","url":null,"abstract":"","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"80 1","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72667973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Different cell types in Neurospora crassa","authors":"G. Bistis, D. D. Perkins, N. Read","doi":"10.4148/1941-4765.1154","DOIUrl":"https://doi.org/10.4148/1941-4765.1154","url":null,"abstract":"Neurospora possesses more cell types than are commonly recognized. We have been able to identify 28 morphologically distinct types. Having the cell types clearly defined will be important for genome annotation, describing new mutant phenotypes, and determining sites of gene expression. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol50/iss1/8 Number 50, 2003 17 Different cell types in Neurospora crassa George N. Bistis, David D. Perkins, and Nick D. Read Department of B iology, Drew University, M adison, NJ 07940, Department of B iological Sciences, Stanford University, Stanford, CA 94305-5020, Department of Cell and Molecular Biology, University of Edinburgh, Rutherford Building, Edinburgh EH8 9QU, U.K. Fungal Genet. Newsl. 50:17-19 Neurospora possesses more cell types than are commonly recognized. We have been able to identify 28 morphologically distinct types. Having the cell types clearly defined will be important for genome annotation, describing new mutant phenotypes, and determining sites of gene expression. ____________________________________________________________________________________ Neurospora is a morphologically complex multicellular organism with many more cell types than the unicellular yeast Saccharomyces. Most workers are familiar with mycelia, macroconidia, perithecia, asci, and ascospores, but the diversity of cell types produced by Neurospora may not be fully appreciated. Now that the products of specific genes can be localized using GFP and o ther fluorescent proteins, attention will be focused increasingly on particular cell types that differ in morphology, physiology, or developmental origin. D istinguishing different cell types is also important for genome annotation. For convenience, we need to use the terms ‘cell’ and ‘cell type’ rather loosely to cover both cellular elements such as hyphae and discrete cells such as spores (see discussion by Read, 1994). The basic undifferentiated, totipotent cellular element is the compartmentalized vegetative hypha at the colony periphery (the leader hypha). Certain other cell types are comprised of differentiated hyphae (e.g., fusion hyphae, ascogonia, trichogynes, ascogenous hyphae, asci, paraphyses, and periphyses). At the other extreme are highly differentiated nonhyphal cells such as ascospores, microconidia, and the different wall cells of protoperithecia and perithecia. Twenty-eight morphologically distinct cell types are listed and described below. Designation of protoperithecia and microconidia as vegetative or sexual is arbitrary. Additional types or subtypes will no doubt be revealed.","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"27 1","pages":"17-19"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78834707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient high-volume cleaning of Aspergillus nidulans cleistothecia using bare fingers.","authors":"T. Hill, D. Loprete, Jared A. Castagna, Samuel O. Weems","doi":"10.4148/1941-4765.1148","DOIUrl":"https://doi.org/10.4148/1941-4765.1148","url":null,"abstract":"Even seasoned workers find it tedious and sometimes frustrating to remove Hülle cells and stray conidia from Aspergillus cleistothecia by rolling them with forceps across an agar surface, particularly when large numbers must be cleaned. It can be even more challenging to teach the skill to others, especially to a whole class of easily discouraged undergraduates, who may be seeing their first high-mag image of forceps tips at the same time as their first view of a cleistothecium. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol50/iss1/2 4 Fungal Genetics Newsletter Efficient high-volume cleaning of Aspergillus nidulans cleistothecia using bare fingers. Terry W. Hill, Darlene M. Loprete, Jared A, Castagna, and Samuel O. Weems. Department of Biology and Department of Chemistry, Rhodes College, Memphis, TN 38112 USA. Even seasoned workers find it tedious and sometimes frustrating to remove Hülle cells and stray conidia from Aspergillus cleistothecia by rolling them with forceps across an agar surface, particularly when large numbers must be cleaned. It can be even more challenging to teach the skill to others, especially to a whole class of easily discouraged undergraduates, who may be seeing their first high-mag image of forceps tips at the same time as their first view of a cleistothecium. The tips of forceps are sharp and rough, and the hands that wield them often shaky. A peridium is no match for an ill-aimed poke or slash. We find, however, that cleistothecia can be cleaned very rapidly, with reduced breakage, and with minimal contamination by doing away with forceps altogether and using instead the tools that nature gave us “at our fingertips”. Indeed they are our fingertips themselves. The method is simply to roll cleistothecia briefly and firmly around the surface of a 4% agar plate (with or without diatomaceous earth per Kaminskyj and Hamer, 1996, Fungal Genetics Newsletter 43:71) beneath a well-cleaned fingertip. A few seconds’ rubbing in a circle about an inch in diameter is all that is required. The pressure of the finger is spread evenly, and the cleistothecia only rarely break. For increased efficiency, several cleistothecia can be rubbed at once beneath a single fingertip. Soap-andwater washing, followed by two or three 10-second immersions of the finger in 95% alcohol, with Kimwipe-drying after each immersion, is sufficient in our experience to reduce levels of bacterial contamination to no more than those observed when using flamed forceps – i.e., essentially none. Published by New Prairie Press, 2017","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"31 1","pages":"4-5"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76077601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vogel's Medium N salts: avoiding the need for ammonium nitrate","authors":"R. L. Metzenberg","doi":"10.4148/1941-4765.1152","DOIUrl":"https://doi.org/10.4148/1941-4765.1152","url":null,"abstract":"Vogel's Medium N salts (Vogel, 1956 Microbiol. Genet. Bull. 13:42-43), supplemented with a carbon source, is widely used for the cultivation of Neurospora and many other fungi. The standard recipe includes ammonium nitrate. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol50/iss1/6 14 Fungal Genetics Newsletter 14 Vogel's Medium N salts: avoiding the need for ammonium nitrate Robert L. Metzenberg. Visiting Prof., Dept. of Chemistry and Biochemistry, University of California, Los Angeles CA 90095. Fungal Genet. News. 50:14 Vogel's Medium N salts (Vogel, 1956 Microbiol. Genet. Bull. 13:42-43), supplemented with a carbon source, is widely used for the cultivation of Neurospora and many other fungi. The standard recipe includes ammonium nitrate. Because the ammonium ion is a reductant and nitrate ion is an oxidant, solid ammonium nitrate is potentially an explosive, as is well known from the disastrous 1947 Texas City accident and from more recent acts of terrorism such as the bombing of the Federal Building in Oklahoma City. Ammonium nitrate can explode without being deliberately detonated. Shipping it, therefore, presents some problems, and even declaring it in a lab inventory may do so in the future. The tendency of the dry salt to become caked in its container invites foolish actions to free up a sample, for example, banging the jar on the edge of a benchtop . The following recipe yields an identical final composition to Vogel's Medium N, but employs potassium nitrate and ammonium phosphate. Since potassium ion is not a reductant and phosphate ion is not an oxidant, the dry ingredients can be regarded as harmless, as, of course, are the dissolved salts. The following recipe is for 1 liter of 50X salts. water 750 ml. Na3 citrate .2H2O 130 g. KNO3 126 g. (NH4)H2PO4 144 g. KH2 PO4 80 g. MgSO4 7 H2O 10 g. CaCl2 .2H2O 5 g. in 20 ml. water; add dropwise. trace elements solution* 5 ml. biotin solution, 0.1 mg/ml 2.5 ml. chloroform to preserve: a few ml. No adjustment of pH is necessary or desirable. *Trace elements: several variants of the following have been published. The differences in these formulations are unlikely to be important. water 95 ml citric acid .H2O 5 g. ZnSO4 .7H2O 5 g. Fe(NH4)2(SO4)2 .6H2O 1 g. CuSO4 .5H2O 250 mg. MnSO4 .H2O 50 mg. H3BO3 50 mg. Na2MoO 4 .2H2O 50 mg. Published by New Prairie Press, 2017","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"16 1","pages":"14-14"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83181762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Split-Marker Recombination for Efficient Targeted Deletion of Fungal Genes","authors":"Natalie L. Catlett, Bee-Na Lee, O. Yoder, B. Turgeon","doi":"10.4148/1941-4765.1150","DOIUrl":"https://doi.org/10.4148/1941-4765.1150","url":null,"abstract":"A commonly used method for fungal gene deletion is introduction of linear DNA consisting of a selectable marker gene flanked on both sides by short stretches of DNA that target a gene of interest (W irsel et al 1996 Curr. Genet 29:241-249). Gene deletion in Cochliobolus heterostrophus and Gibberella zeae occurs efficiently with this approach. To facilitate deletion construct synthesis, we have applied the \"split-marker” deletion strategy previously developed for Saccharomyces cerevisiae (Fairhead et al. 1996 Y east 12:1439-57; Fairhead et al. 1998 Gene 223:33-46). Here, we describe both fusion PCR-based and plasmid-based deletion methods using this strategy with PEG-mediated protoplast transformation (Turgeon et al, 1985 M ol. Gen. Genet. 201:450-453). These methods are predicted to work well with any transformable fungus that undergoes homologous recombination between chromosomal and introduced DNA sequences.","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"12 1","pages":"9-11"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79469101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation of genomic DNA from acetone-dried Aspergillus mycelia","authors":"N. Punekar, Santhosh K. P. Kumar, T. Jayashri, R. Anuradha","doi":"10.4148/1941-4765.1153","DOIUrl":"https://doi.org/10.4148/1941-4765.1153","url":null,"abstract":"A simple acetone-drying protocol was adopted to replace the lyophilization step while isolating genomic DNA from Aspergillus mycelia. This DNA is suitable for PCR, restriction enzyme digestion and Southern blot analysis with digoxigenin-labeled DNA probes. Acetone drying/ preservation can be a useful method in the molecular analysis of fungal DNA samples. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol50/iss1/7","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"83 1","pages":"15-16"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73241823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}