The q-rung linear Diophantine fuzzy set is a recently developed tool to handle with uncertain and awkward information in real-life issues and is applicable where reference parameter-based opinions. Similarity measures are distance with dimensions representing features of the objects. Keeping the advantages of the above analysis, this paper proposes similarity measures based on exponential function for q-rung linear Diophantine fuzzy sets and thus presents the first formulas for calculating the similarity coefficient between two q-rung linear Diophantine fuzzy sets. These proposed similarity measures are applied to the clustering problem and the results are analyzed. In addition, the comparison outputs of the new similarity measures are discussed to ensure their good performance.
{"title":"Exponential Function-Based Similarity Measures for q-Rung Linear Diophantine Fuzzy Sets and Their Application to Clustering Problem","authors":"S. Petchimuthu, Hüseyin Kamacı","doi":"10.35378/gujs.1013358","DOIUrl":"https://doi.org/10.35378/gujs.1013358","url":null,"abstract":"The q-rung linear Diophantine fuzzy set is a recently developed tool to handle with uncertain and awkward information in real-life issues and is applicable where reference parameter-based opinions. Similarity measures are distance with dimensions representing features of the objects. Keeping the advantages of the above analysis, this paper proposes similarity measures based on exponential function for q-rung linear Diophantine fuzzy sets and thus presents the first formulas for calculating the similarity coefficient between two q-rung linear Diophantine fuzzy sets. These proposed similarity measures are applied to the clustering problem and the results are analyzed. In addition, the comparison outputs of the new similarity measures are discussed to ensure their good performance.","PeriodicalId":12615,"journal":{"name":"gazi university journal of science","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47196815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Obtaining and storing large amounts of data have become easier with the rapidly developing information technologies (IT). However, the data generated and collected, which are irrelevant in and of themselves, become useful only when they are analyzed for a specific reason. Data mining may transform raw data into useful information. In the present study, classification and analysis of denim fabric quality characteristics according to denim fabric production parameters were carried out. The present study proposes a new classification rule inference algorithm. The suggested approach is mostly based on Artificial Bee Colony Optimization (ABC), a swarm intelligence meta-heuristic. In each step of the algorithm, there are two phases called the employed bee phase and the onlooker bee phase. This algorithm has been compared with the classification algorithms in the related literature. This proposed algorithm is a new data mining tool that intelligently combines various metaheuristic and neural networks and can generate classification rules. The results indicate that the proposed data mining algorithms may be highly useful in determining weight and width in denim fabric manufacture.
{"title":"Developed ABCLASS-Miner Classification Algorithm Based Rule Extraction for Denim Fabrics","authors":"Gözde Katircioğlu, Emel Kizilkaya Aydoğan, Esra Akgul, Yılmaz Delice","doi":"10.35378/gujs.1185130","DOIUrl":"https://doi.org/10.35378/gujs.1185130","url":null,"abstract":"Obtaining and storing large amounts of data have become easier with the rapidly developing information technologies (IT). However, the data generated and collected, which are irrelevant in and of themselves, become useful only when they are analyzed for a specific reason. Data mining may transform raw data into useful information. In the present study, classification and analysis of denim fabric quality characteristics according to denim fabric production parameters were carried out. The present study proposes a new classification rule inference algorithm. The suggested approach is mostly based on Artificial Bee Colony Optimization (ABC), a swarm intelligence meta-heuristic. In each step of the algorithm, there are two phases called the employed bee phase and the onlooker bee phase. This algorithm has been compared with the classification algorithms in the related literature. This proposed algorithm is a new data mining tool that intelligently combines various metaheuristic and neural networks and can generate classification rules. The results indicate that the proposed data mining algorithms may be highly useful in determining weight and width in denim fabric manufacture.","PeriodicalId":12615,"journal":{"name":"gazi university journal of science","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48598620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Merve Güneş, B. Yalçın, Ayşen Yağmur Kurşun, Ghada Tagorti, Emre Yavuz, E. Akarsu, N. Kaya, Bülent Kaya
Biomaterials are engineering products that are widely used in many medical fields such as orthopaedic applications, facial and maxillofacial surgery, artificial heart parts, metal parts and implantable devices. These materials are widely used in medicine because they are compatible with the organism, are not allergic, and are resistant to physical and chemical factors. Hydroxyapatites are bioactive calcium phosphate ceramics that are compatible with tissues. To increase their bioactivity, nano-sized hydroxyapatite has been produced. Although there are advantages to the use of nanoparticles in the medical field, the potentially toxic effects of these materials on the environment and human health are of concern. It is also one of the topics to be investigated whether the different forms of the same nanoparticle will cause differences in genotoxicity. In this study, the potential genotoxic effects of rod and round forms of nano-sized hydroxyapatites (nHAs) were evaluated in Allium cepa Single Cell Gel Electrophoresis (COMET) method. According to the results, the round form of nHA in the A. cepa meristem root tip cells caused statistically significant genotoxicity at 25 ppm concentration in terms of tail density and tail moment. This study showed small-sized-nanohydroxyapatite-induced genotoxicity and cell death in A. cepa. This study has shown that the physical properties of nanoparticles affect potential toxicity mechanisms.
{"title":"The Effects of Rod and Round-Like Nanohydroxyapatites on Allium cepa Root Meristem Cells","authors":"Merve Güneş, B. Yalçın, Ayşen Yağmur Kurşun, Ghada Tagorti, Emre Yavuz, E. Akarsu, N. Kaya, Bülent Kaya","doi":"10.35378/gujs.1218829","DOIUrl":"https://doi.org/10.35378/gujs.1218829","url":null,"abstract":"Biomaterials are engineering products that are widely used in many medical fields such as orthopaedic applications, facial and maxillofacial surgery, artificial heart parts, metal parts and implantable devices. These materials are widely used in medicine because they are compatible with the organism, are not allergic, and are resistant to physical and chemical factors. Hydroxyapatites are bioactive calcium phosphate ceramics that are compatible with tissues. To increase their bioactivity, nano-sized hydroxyapatite has been produced. Although there are advantages to the use of nanoparticles in the medical field, the potentially toxic effects of these materials on the environment and human health are of concern. It is also one of the topics to be investigated whether the different forms of the same nanoparticle will cause differences in genotoxicity. In this study, the potential genotoxic effects of rod and round forms of nano-sized hydroxyapatites (nHAs) were evaluated in Allium cepa Single Cell Gel Electrophoresis (COMET) method. According to the results, the round form of nHA in the A. cepa meristem root tip cells caused statistically significant genotoxicity at 25 ppm concentration in terms of tail density and tail moment. This study showed small-sized-nanohydroxyapatite-induced genotoxicity and cell death in A. cepa. This study has shown that the physical properties of nanoparticles affect potential toxicity mechanisms.","PeriodicalId":12615,"journal":{"name":"gazi university journal of science","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46673680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This paper describes an analysis of lengthwise fracture in a viscoelastic inhomogeneous cantilever beam configuration under strain that is a sinusoidal function of time. The mechanical behaviour of the beam is treated by a viscoelastic model having two linear springs and a linear dashpot. The beam is continuously inhomogeneous along its thickness. Therefore, the modulii of elasticity of the springs and the coefficient of viscosity of the dashpot vary smoothly along the thickness of the beam. The compliance method is applied to derive a solution of the strain energy release rate for the lengthwise crack in the beam structure. The J-integral is used for verification. The stress-strain-time dependence of the viscoelastic model is applied for describing the behavior of the beam when obtaining solutions of the strain energy release rate and the J-integral. Solutions are derived for both positive and negative angle of rotation of the lower crack arm free end (when the angle is positive, the upper crack arm is free of stresses, while at negative angle both crack arms are loaded). The effects of various factors including the sign of the angle of rotation on the strain energy release rate are analyzed.
{"title":"Lengthwise Fracture Analysis of Inhomogeneous Viscoelastic Cantilever Beam Subjected to Sinusoidal Strains","authors":"Victor RİZOV","doi":"10.35378/gujs.1062749","DOIUrl":"https://doi.org/10.35378/gujs.1062749","url":null,"abstract":"This paper describes an analysis of lengthwise fracture in a viscoelastic inhomogeneous cantilever beam configuration under strain that is a sinusoidal function of time. The mechanical behaviour of the beam is treated by a viscoelastic model having two linear springs and a linear dashpot. The beam is continuously inhomogeneous along its thickness. Therefore, the modulii of elasticity of the springs and the coefficient of viscosity of the dashpot vary smoothly along the thickness of the beam. The compliance method is applied to derive a solution of the strain energy release rate for the lengthwise crack in the beam structure. The J-integral is used for verification. The stress-strain-time dependence of the viscoelastic model is applied for describing the behavior of the beam when obtaining solutions of the strain energy release rate and the J-integral. Solutions are derived for both positive and negative angle of rotation of the lower crack arm free end (when the angle is positive, the upper crack arm is free of stresses, while at negative angle both crack arms are loaded). The effects of various factors including the sign of the angle of rotation on the strain energy release rate are analyzed.","PeriodicalId":12615,"journal":{"name":"gazi university journal of science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135223036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Turbines are mechanical devices that convert fluid motion into rotational motion. In this study, water wave is used as fluid. Ansys Fluent's Volume Of Fluid (VOF) model is used and regular waves are obtained to analyze the movement of the turbine in the wave tank. The force, pressure, moment and speed of the turbine are important to obtain electrical energy from wave energy. For this reason, in this study, the change of these parameters with the effect of the wave is examined. It is observed that these parameter values increased with increasing wave height. It is observed that the dynamic pressure and moment of the turbine rise with the increase of the wavelength. However, the speed of rotation of the turbine decreased with the increase of the wavelength. The force coming to the blades did not change with the increase of the wavelength, it only caused a delay in time. However, it was observed that a very high force was applied to the turbine blades with a wave height of 2 m and this value reached 8000 N. In addition, the maximum turbine speed was obtained at a wavelength of 2 m and a wavelength of 20 m, reaching 87 mm / s.
{"title":"Turbine Type Rotary Wave Energy Converter Performance","authors":"Perihan Karaköse, A. Koca","doi":"10.35378/gujs.1160148","DOIUrl":"https://doi.org/10.35378/gujs.1160148","url":null,"abstract":"Turbines are mechanical devices that convert fluid motion into rotational motion. In this study, water wave is used as fluid. Ansys Fluent's Volume Of Fluid (VOF) model is used and regular waves are obtained to analyze the movement of the turbine in the wave tank. The force, pressure, moment and speed of the turbine are important to obtain electrical energy from wave energy. For this reason, in this study, the change of these parameters with the effect of the wave is examined. It is observed that these parameter values increased with increasing wave height. It is observed that the dynamic pressure and moment of the turbine rise with the increase of the wavelength. However, the speed of rotation of the turbine decreased with the increase of the wavelength. The force coming to the blades did not change with the increase of the wavelength, it only caused a delay in time. However, it was observed that a very high force was applied to the turbine blades with a wave height of 2 m and this value reached 8000 N. In addition, the maximum turbine speed was obtained at a wavelength of 2 m and a wavelength of 20 m, reaching 87 mm / s.","PeriodicalId":12615,"journal":{"name":"gazi university journal of science","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42975699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cell growth inhibition is generally handled as a measure of toxicity. Shortly, more toxicity implies more growth inhibition. Then, the question arises; How to calculate & evaluate cell growth inhibition in a universal manner? Actually, the method for calculating growth inhibition is not considered to be a central issue, in general. There are various approaches for calculating cell growth. Among these approaches, two of them are highly easy and popular, subtraction-based and division-based calculations. However, these two methods for the calculation of cell growth do not strongly reflect the nature of cell growth. Alternatively, the use of a doubling number-based formulation can provide a better approach and performance in the evaluation of cell growth and cell growth inhibition unless the culture attains the confluent status. Here, we discussed different methods of growth calculation which we applied to the study of “growth inhibition of BY-2 cells under Cd exposure”.
{"title":"Use of Doubling Number as an Arithmetic Measure of Plant Cell Growth and Metal-Induced Cell Growth Inhibition","authors":"M. Metin, T. Kawano","doi":"10.35378/gujs.1244209","DOIUrl":"https://doi.org/10.35378/gujs.1244209","url":null,"abstract":"Cell growth inhibition is generally handled as a measure of toxicity. Shortly, more toxicity implies more growth inhibition. Then, the question arises; How to calculate & evaluate cell growth inhibition in a universal manner? Actually, the method for calculating growth inhibition is not considered to be a central issue, in general. There are various approaches for calculating cell growth. Among these approaches, two of them are highly easy and popular, subtraction-based and division-based calculations. However, these two methods for the calculation of cell growth do not strongly reflect the nature of cell growth. Alternatively, the use of a doubling number-based formulation can provide a better approach and performance in the evaluation of cell growth and cell growth inhibition unless the culture attains the confluent status. Here, we discussed different methods of growth calculation which we applied to the study of “growth inhibition of BY-2 cells under Cd exposure”.","PeriodicalId":12615,"journal":{"name":"gazi university journal of science","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44047569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The main goal of health institutions is to provide the health services needed by society at the desired quality with the lowest possible cost. Considering the total number of employees in health institutions, nurse assignment and scheduling have an essential role in increasing efficiency and improving service quality due to the one-to-one interaction of nurses with patients. This study proposes a nurse scheduling model based on nurses’ skill levels incorporated into a decision support system. The skill level of nurses is assessed using Analytic Hierarchy Process and Technique for Order Preference by Similarity to Ideal Solution method based on eight criteria. The nurse scheduling problem is then modeled with 0-1 Goal Programming, considering the skill assessment as a constraint. The applicability of the proposed model is examined for the assignment and scheduling conditions of nurses at the 3rd level of surgical intensive care in a general hospital, and the valuable aspects of the proposed approach are discussed. When the proposed solution is compared with the current situation, it is realized that one nurse is saved without worsening the constraints.
{"title":"A Decision Support System For Skill-Based Nurse Scheduling In An Intensive Care Unit","authors":"Orhan Parildar, Ç. Akyürek, D. Akay","doi":"10.35378/gujs.1218158","DOIUrl":"https://doi.org/10.35378/gujs.1218158","url":null,"abstract":"The main goal of health institutions is to provide the health services needed by society at the desired quality with the lowest possible cost. Considering the total number of employees in health institutions, nurse assignment and scheduling have an essential role in increasing efficiency and improving service quality due to the one-to-one interaction of nurses with patients. This study proposes a nurse scheduling model based on nurses’ skill levels incorporated into a decision support system. The skill level of nurses is assessed using Analytic Hierarchy Process and Technique for Order Preference by Similarity to Ideal Solution method based on eight criteria. The nurse scheduling problem is then modeled with 0-1 Goal Programming, considering the skill assessment as a constraint. The applicability of the proposed model is examined for the assignment and scheduling conditions of nurses at the 3rd level of surgical intensive care in a general hospital, and the valuable aspects of the proposed approach are discussed. When the proposed solution is compared with the current situation, it is realized that one nurse is saved without worsening the constraints.","PeriodicalId":12615,"journal":{"name":"gazi university journal of science","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47871289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Halogenated compounds, especially fluorine and chlorine, play a key role in drug development. They account for a large proportion of all approved drug molecules. The importance of these two halogens stems from their remarkable effects on biological activity and pharmacokinetic properties. The study presented here aims to give the results obtained by the DFT methods and in silico medicinal evaluations of a newly synthesized small molecule. The small molecule belongs to the Schiff base class of organic compounds and is substituted with halogen atoms. The tetrahalogenated compound (THSB) Schiff base, 2-(((2,4-dichlorophenyl)imino)methyl)-3,4-difluorophenol, was first synthesized via the classical condensation method and then characterized by spectroscopic techniques. The THSB optimized by the B3LYP method was evaluated in terms of geometrical parameters, surface area (MEP and Hirshfeld analysis) and secondary interaction analysis, NLO properties, and electronic properties (HOMO-LUMO and UV-Vis). Second, THSB was evaluated regarding medicinal chemistry, physicochemical and pharmacokinetic properties, and toxicity (ADMET). Then, we comprehensively investigated the potential biological targets of THSB. Using the results of the SwissSimilarity analysis, we investigated the antagonistic effects of THSB against serotonin 5-HT2A and dopamine D2 receptors. Docking results were compared with the known antipsychotics, clozapine and risperidone. THSB showed a higher antagonistic effect than clozapine for the D2 receptor. However, risperidone proved to be the most effective antagonist for both targets. The binding energies of THSB, risperidone, and clozapine were -8.30, -11.84, and -8.07 kcal/mol, respectively, for D2; those of THSB, risperidone, and clozapine were -6.94, -11.47, and -10.10 kcal/mol, respectively, for 5-HT2A.
{"title":"2-(((2,4-Dichlorophenyl)imino)methyl)-3,4-difluorophenol: X-ray, DFT, MEP, HOMO-LUMO, NLO, Hirshfeld surfaces, ADMET profiling, target identification, antipsychotic activity against dopamine D2 and serotonin 5-HT2A receptors","authors":"Songül Şahin","doi":"10.35378/gujs.1241638","DOIUrl":"https://doi.org/10.35378/gujs.1241638","url":null,"abstract":"Halogenated compounds, especially fluorine and chlorine, play a key role in drug development. They account for a large proportion of all approved drug molecules. The importance of these two halogens stems from their remarkable effects on biological activity and pharmacokinetic properties. The study presented here aims to give the results obtained by the DFT methods and in silico medicinal evaluations of a newly synthesized small molecule. The small molecule belongs to the Schiff base class of organic compounds and is substituted with halogen atoms. The tetrahalogenated compound (THSB) Schiff base, 2-(((2,4-dichlorophenyl)imino)methyl)-3,4-difluorophenol, was first synthesized via the classical condensation method and then characterized by spectroscopic techniques. The THSB optimized by the B3LYP method was evaluated in terms of geometrical parameters, surface area (MEP and Hirshfeld analysis) and secondary interaction analysis, NLO properties, and electronic properties (HOMO-LUMO and UV-Vis). Second, THSB was evaluated regarding medicinal chemistry, physicochemical and pharmacokinetic properties, and toxicity (ADMET). Then, we comprehensively investigated the potential biological targets of THSB. Using the results of the SwissSimilarity analysis, we investigated the antagonistic effects of THSB against serotonin 5-HT2A and dopamine D2 receptors. Docking results were compared with the known antipsychotics, clozapine and risperidone. THSB showed a higher antagonistic effect than clozapine for the D2 receptor. However, risperidone proved to be the most effective antagonist for both targets. The binding energies of THSB, risperidone, and clozapine were -8.30, -11.84, and -8.07 kcal/mol, respectively, for D2; those of THSB, risperidone, and clozapine were -6.94, -11.47, and -10.10 kcal/mol, respectively, for 5-HT2A.","PeriodicalId":12615,"journal":{"name":"gazi university journal of science","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42338907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The harmony of the Weibull Distribution Function (WDF) and real data from three different places was investigated in this research. The coefficients of the WDF were calculated using the Maximum Likelihood Method (MLM) in the Adana, Osmaniye, and Hatay regions. One of the main goals of this research is to see how the MLM's performance varies in different places with different mean wind speeds. For the purpose of justifying the MLM's performance, the statistical fits of the computed and measured hourly wind speed data were analyzed. The performance of the indicated approach in all three selected locations was evaluated using the Root Mean Square Error (RMSE), Coefficient of Determination (R2), and Mean Percentage Error (MPE) error analysis tests, according to the results. Also estimated for all three regions were wind power densities, which are one of the most essential metrics for estimating a region's wind energy potential. WDF power densities were estimated and compared to real wind power densities generated from measured wind data in three different places. The performance of the strategy described in this paper was investigated in depth in various places with varying geographic characteristics. In addition, in the same years, the performance of the chosen approach was evaluated in detail in three distinct places, and it was seen how geographical factors affected the method's performance.
{"title":"Calculating Weibull coefficients using the Maximum Likelihood Method and comparing performance across sites","authors":"Alper Kaplan","doi":"10.35378/gujs.1092617","DOIUrl":"https://doi.org/10.35378/gujs.1092617","url":null,"abstract":"The harmony of the Weibull Distribution Function (WDF) and real data from three different places was investigated in this research. The coefficients of the WDF were calculated using the Maximum Likelihood Method (MLM) in the Adana, Osmaniye, and Hatay regions. One of the main goals of this research is to see how the MLM's performance varies in different places with different mean wind speeds. For the purpose of justifying the MLM's performance, the statistical fits of the computed and measured hourly wind speed data were analyzed. The performance of the indicated approach in all three selected locations was evaluated using the Root Mean Square Error (RMSE), Coefficient of Determination (R2), and Mean Percentage Error (MPE) error analysis tests, according to the results. Also estimated for all three regions were wind power densities, which are one of the most essential metrics for estimating a region's wind energy potential. WDF power densities were estimated and compared to real wind power densities generated from measured wind data in three different places. The performance of the strategy described in this paper was investigated in depth in various places with varying geographic characteristics. In addition, in the same years, the performance of the chosen approach was evaluated in detail in three distinct places, and it was seen how geographical factors affected the method's performance.","PeriodicalId":12615,"journal":{"name":"gazi university journal of science","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47664736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, to enhance protease production, the wild type of Bacillus subtilis E6-5 was mutagenized by random mutagenesis using ultraviolet radiation and ethidium bromide. After combined treatment, several mutants were obtained. Among these mutants, the mutant strain with the largest proteolytic zone diameter (25 mm) was selected and named Bacillus subtilis ATA38. The enzyme production capacity of the obtained mutant was tested and the mutant strain (404 IU/mL at 24 hours) produced 6.7 times more enzyme than the parental strain (60 IU/mL at 32 hours). The effects of some important parameters in the growth medium on enzyme production were examined. The best carbon, organic nitrogen and metal ion were obtained with wheat starch (525 IU/mL), meat extract (850 IU/mL) and KCl+CaCl2 (548 IU/mL), respectively. pH 6.0, 37°C, 200 rpm, inoculum age 18 hours and inoculation amount 1% were obtained as the best physical factors.To further increase the yield, the best nutritional and physical parameters were combined to create a new modified medium. It was determined that the enzyme yield with mutant strain increased 2.7 times in the modified medium (1096 IU/mL) compared to the control (404 U/mL). The mutant strain (1096 IU/mL) showed an 18.2-fold increase in production compared to the wild type (60 IU/mL) in the modified medium. Protease enzyme obtained from ATA38 mutant strain may have great potential in industry for different purposes.
{"title":"Obtaining Efficient Mutant from the Wild Type Bacillus subtilis E6-5 by Physical and Chemical Mutagenesis for High Efficiency Protease Production, Optimizing the Mutant's Culture Medium","authors":"Büşra Özalpar, E. Demirkan, Tuba Sevgi̇","doi":"10.35378/gujs.1191006","DOIUrl":"https://doi.org/10.35378/gujs.1191006","url":null,"abstract":"In this study, to enhance protease production, the wild type of Bacillus subtilis E6-5 was mutagenized by random mutagenesis using ultraviolet radiation and ethidium bromide. After combined treatment, several mutants were obtained. Among these mutants, the mutant strain with the largest proteolytic zone diameter (25 mm) was selected and named Bacillus subtilis ATA38. The enzyme production capacity of the obtained mutant was tested and the mutant strain (404 IU/mL at 24 hours) produced 6.7 times more enzyme than the parental strain (60 IU/mL at 32 hours). The effects of some important parameters in the growth medium on enzyme production were examined. The best carbon, organic nitrogen and metal ion were obtained with wheat starch (525 IU/mL), meat extract (850 IU/mL) and KCl+CaCl2 (548 IU/mL), respectively. pH 6.0, 37°C, 200 rpm, inoculum age 18 hours and inoculation amount 1% were obtained as the best physical factors.To further increase the yield, the best nutritional and physical parameters were combined to create a new modified medium. It was determined that the enzyme yield with mutant strain increased 2.7 times in the modified medium (1096 IU/mL) compared to the control (404 U/mL). The mutant strain (1096 IU/mL) showed an 18.2-fold increase in production compared to the wild type (60 IU/mL) in the modified medium. Protease enzyme obtained from ATA38 mutant strain may have great potential in industry for different purposes.","PeriodicalId":12615,"journal":{"name":"gazi university journal of science","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43754606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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