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Phenotypic and genetic differentiation of qualitative traits in sheep ecotype from Cameroon Western Highlands 喀麦隆西部高地生态型绵羊质量性状的表型和遗传分化
Pub Date : 2020-06-25 DOI: 10.46325/GABJ.V4I2.756
Yannick Tadakeng Djoufack, F. Meutchieye, Y. Manjeli
This study was carried out in the highlands zone, west region of Cameroon to evaluate the distribution and frequency of sheep qualitative traits. Four hundred and three (403) adult sheep were randomly sampled in the study area. The qualitative traits observed were the presence or not of toggles, beard, horns and the sheep coat color (white, black, white-black and white-brown). The main results showed that the traits observed were variable according to the locality and sex of the animal. The sheep from the Bamboutos Division have the highest toggles frequency (21.69%) compared to those of the Noun Division whose frequency was the lowest (11.67%). Toggles were present in both sexes with higher frequency of 18.18% in females compared to the males (9.47%). Beard was almost non-existent in the two localities as well as in the two sexes. Horns were present in sheep of all the investigated localities and in both sexes, with higher frequency in males (90.53%) compared to females (8.12%). The action of the gene responsible for the color of the coat was variable as far as any locality was concerned. The predominant color observed was white. The gene frequencies recorded for the presence of the toggles (Waw), beard (Brb) and horns (HoP) were 0.06%, 0.00% and 0.35%, respectively. These values were far below the expected Mendelian value of 0.75. These low frequencies observed would indicate that the sheep populations of the highlands of the west region of Cameroon were variegated without proper actions of artificial selection, opening ways for genetic improvement.
本研究在喀麦隆西部高原地区对绵羊质量性状的分布和频率进行了评价。在研究区随机抽取成年羊403只。观察到的质量性状包括是否有毛梗、胡须、羊角和被毛颜色(白色、黑色、白黑色和白棕色)。主要结果表明,所观察到的性状根据动物的位置和性别而变化。竹类羊的切换频率最高(21.69%),名词类羊的切换频率最低(11.67%)。两性均存在切换,女性切换频率为18.18%,高于男性切换频率(9.47%)。在这两个地区和两性中,胡须几乎不存在。在所调查的所有地区,雌雄羊均存在角,其中公羊(90.53%)高于母羊(8.12%)。就任何地方而言,负责被毛颜色的基因的作用是可变的。观察到的主要颜色是白色。基因频率记录显示,突变体(Waw)、胡须(Brb)和角(HoP)的基因频率分别为0.06%、0.00%和0.35%。这些值远低于预期的孟德尔值0.75。观察到的这些低频率表明,喀麦隆西部高地的绵羊种群没有经过适当的人工选择,从而为遗传改良开辟了道路。
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引用次数: 1
Comparative study on morphometric traits of local chicken (gallus domesticus L.) in wilaya of Adrar Adrar wilaya地区鸡(gallus domesticus L.)形态计量性状的比较研究
Pub Date : 2020-06-25 DOI: 10.46325/GABJ.V4I2.759
A. Ameur, Abdelhakim Barkaoui, Zahra Guermoudi,, Ikrame Mellouk, S. Gaouar
A study was conducted in the wilaya of Adrar in Algeria (a part of Sahara) to describe the phenotype and morphometric characteristics of local chickens. Six regions namely (part of the wilaya of Adrar) Tamentit, Zawyet Elkenta, Wlad Ahmed, Adrar city, Anezjmir and Founoghil were selected for data collection. A field survey was used to extract information from the farmers concerning production attributes of their local chickens. Measurements on body weight, tarsal length and width, high Length, thigh width, bill length, spout width, total length, breast width and eggs number per year were taken on 89 mature chickens (69 females and 20 males). The results obtained indicate that there exists significant variation between regions with respect to some production attributes as well as body weight and other body measurements. Fonoughil and Adrar city regions had the heaviest chickens (2.54kg-2.40kg respectively) while Zawyet Elkenta had the lightest birds (1.8kg). Overall, male chickens were higher than female birds with respects to all attributes studied. Body weight appeared to be highly positively correlated to some other body measurements considered in this study. The observed variation about the morphometric traits in the present study illustrate by the principal component analysis suggests that there are two groups, the first of which is represented by individuals from the Adar city region, in the other hand the second grouped all the rest of the 5 regions. There is need to pay particular attention to the Adrar city region chicken population for possible consideration for conservation and selection plan.
在阿尔及利亚的Adrar wilaya(撒哈拉沙漠的一部分)进行了一项研究,以描述当地鸡的表型和形态特征。6个地区(阿德拉省的一部分)Tamentit、Zawyet Elkenta、Wlad Ahmed、阿德拉市、Anezjmir和Founoghil被选中进行数据收集。采用实地调查的方法,从农民那里获取有关当地鸡生产属性的信息。对89只成年鸡(母鸡69只,公鸡20只)进行了年体重、跗骨长宽、高长、大腿宽、喙长、嘴宽、全长、胸宽和产蛋数的测定。结果表明,在某些生产属性以及体重和其他身体测量方面,地区之间存在显著差异。Fonoughil和Adrar地区的鸡最重(分别为2.54kg-2.40kg),而Zawyet Elkenta地区的鸡最轻(1.8kg)。总体而言,雄鸡的各项指标均高于雌鸡。体重似乎与本研究中考虑的其他一些身体测量高度正相关。主成分分析表明,在本研究中观察到的形态计量性状的变异可以分为两个类群,第一类类群以阿达尔市地区的个体为代表,第二类类群是5个地区的全部个体。有必要特别注意阿德拉尔市地区的鸡种群,以便可能考虑保护和选择计划。
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引用次数: 1
Yield stability and test location representativeness in foxtail millet [Setaria italica (L.) Beauv.] genotypes 谷子产量稳定性及试验地代表性测定。)基因型
Pub Date : 2020-06-25 DOI: 10.46325/GABJ.V4I2.753
M. Kandel, N. Dhami, T. R. Rijal, Jiban Shrestha
Development of a crop variety with high grain yield and stability in test locations is an important part of crop breeding program. Thirteen foxtail millet genotypes were evaluated in randomized complete block design with three replications in Jumla and Dolakha, Nepal from April to August in three consecutive years 2017, 2018, and 2019. The objective of this study was to analyze grain yield stability and adaptability of foxtail millet genotypes applying genotype main effect plus genotype by environment interaction (GGE) biplot analysis. The results showed that grain yields were significantly (P<0.05) affected by the environment (E), genotype (G), and their interaction (G×E). The genotype HUMLA-163 had regression coefficient equal to unity (b=1.01), thus this genotype had average adaptation to all environments whereas HUMLA-213 (b=1.18) and HUMLA-252 (b=1.19) had regression coefficients greater than unity, thus these genotypes were more adapted to favorable environments. GGE biplot showed that HUMLA-163 was more stable and adaptive genotype. Thus, genotype HUMLA-163 is recommended for release as a variety to improve foxtail millet production in hilly region of Nepal.
培育高产、稳定的作物品种是作物育种计划的重要组成部分。采用随机完全区组设计,于2017年、2018年和2019年连续3年4 - 8月在尼泊尔的Jumla和Dolakha对13种谷子基因型进行评价。采用环境互作(GGE)双图分析方法,对谷子基因型主效应加基因型的产量稳定性和适应性进行了研究。结果表明,环境(E)、基因型(G)及其互作(G×E)对籽粒产量有显著影响(P<0.05)。基因型HUMLA-163的回归系数等于1 (b=1.01),表明该基因型对所有环境的适应性均为平均,而基因型HUMLA-213 (b=1.18)和基因型HUMLA-252 (b=1.19)的回归系数大于1,表明其对有利环境的适应性更强。GGE双图显示,HUMLA-163基因型更稳定,适应性更强。因此,基因型HUMLA-163被推荐作为提高尼泊尔丘陵地区谷子产量的品种。
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引用次数: 3
General characteristics of goat milk cheese (Feta) in the region of Tlemcen, Algeria 阿尔及利亚特莱姆森地区羊奶奶酪(菲达)的一般特征
Pub Date : 2020-06-25 DOI: 10.46325/GABJ.V4I2.760
Ikram Djebli, A. Ameur, S. Gaouar
Goat milk, whose production is starting to develop in Algeria in recent years, has a number of advantages that even allow it to substitute cow's milk. It is a source of health benefits for humans; it contains more vitamins with a significant cheese yield than cow's milk. Our present study aims to study physicochemical, microbiological and for the first time trials of a fresh cheese (Feta) made from locally selected goat's milk in the region of Tlemcen and its 10 regions namely: Ouled Mimoun, Terny, Sebdou, Ain El houte, Remchi, Bensakrane, Maghnia, Sabra, Nedroma and Zenata. 27 goats from the local population were used for this study. The physicochemical quality (fat, density, conductivity, defatted dry extract, temperature, protein, mineral salts and lactose) of the milk sampled was measured using a LACTOSCAN Milk-Analyzer. Four flora (total germ, fecal coliform, Staphylococcus aureus and salmonella) were chosen to test the microbiological quality of the milk. The results obtained show that the good physicochemical quality of milk studied in all regions except the two regions of Nedroma and Maghnia showing a high fat content. A total absence of contaminations for the three types of fecal coliform flora, Staphylococcus aureus and Salmonella nevertheless for the total germs are found in all sampled regions. These results show that healthy goats hence the good practice of milking is applied during sampling. The manufacture of cheese type feta based on goat's milk was made in good conditions (raw material, hygiene, mechanical work ...) which led to a cheese of microbiological quality and nutritional and an interesting yield. The good breeding condition with a balanced diet gives a good quality of the milk and consequences of these results give an excellent forming thanks to the professional techniques of manufacturing a good feta cheese.
羊奶的生产近年来在阿尔及利亚开始发展,它有许多优点,甚至可以代替牛奶。它是人类健康益处的来源;它含有比牛奶更多的维生素和显著的奶酪产量。我们目前的研究旨在研究特莱姆森地区及其10个地区(即:Ouled Mimoun, Terny, Sebdou, Ain El houte, Remchi, Bensakrane, Maghnia, Sabra, Nedroma和Zenata)的当地精选羊奶制成的新鲜奶酪(Feta)的理化,微生物学和首次试验。该研究使用了当地人口中的27只山羊。使用乳质分析仪测量所取样牛奶的理化品质(脂肪、密度、电导率、脱脂干提取物、温度、蛋白质、无机盐和乳糖)。选取总菌群、粪大肠菌群、金黄色葡萄球菌和沙门氏菌4种菌群对牛奶进行微生物学质量检测。结果表明,除Nedroma和Maghnia两个地区外,所有地区的牛奶理化品质都很好,脂肪含量高。三种类型的粪便大肠菌群、金黄色葡萄球菌和沙门氏菌完全没有污染,但在所有采样地区都发现了总细菌。这些结果表明,健康的山羊,因此,在抽样期间采用了良好的挤奶做法。在良好的条件下(原料、卫生、机械工作……),以羊奶为基础生产奶酪型羊奶酪,这使得奶酪具有微生物质量和营养价值,产量也很可观。良好的饲养条件和均衡的饮食使牛奶质量良好,这些结果的结果是由于制造优质羊奶酪的专业技术而形成了优良的成型。
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引用次数: 2
Exploitation of the common guinea fowl (Numida meleagris) in the Northern region of Cameroon 喀麦隆北部地区普通珍珠鸡(Numida meleagris)的开发
Pub Date : 2020-06-25 DOI: 10.46325/GABJ.V4I2.755
Jean Massawa, Francis Djiotsa Dongmo, F. Meutchieye
Family poultry is an easily accessible source of income and animal protein for low-income populations in developing countries. This study was designed to describe the production system of the guinea fowlin the north region of Cameroon. To achieve this objective, a structured questionnaire was submitted to 72 guinea fowl keepers selected randomly in 7 localities. It emerged that guinea fowl farming is mostly a male business (77.78%). The average flock size is between 5-10 adult animals and the main production objectivesare consumption and sales (69.44%). The flocks are constituted from natural incubations carried out by local hens (88.88%); egg production is seasonal and poor, and almost all incubated eggs come from local markets (84.5%), individual collection in the surrounding groves and domestic spawning. Guinea fowls are in a scavenging system (66.66%) and merely housed. Birds often benefit from few handfuls of cereals (corn seeds and millet) and home wastes. Keets’mortality rate is higher (80.55%) among those aged between 8 to12 days. Majority of guinea fowls’keepers (76%) do not practice any health monitoring. The technical and financial constraintsare the major challenges to the development of guinea fowl production. The organization of producers, the dissemination of appropriate technologies and support with enabling policies and private sectors would improve the overallproduction performance and thus the rational exploitation of this native genetic resource.
对于发展中国家的低收入人群来说,家庭家禽是一种容易获得的收入和动物蛋白来源。本研究旨在描述喀麦隆北部地区珍珠鸡的生产系统。为了实现这一目标,向7个地区随机选择的72名珍珠鸡饲养者提交了一份结构化问卷。结果显示,珍珠鸡养殖业主要是男性(77.78%)。平均畜群规模为5 ~ 10只,主要生产目标为消费和销售(69.44%)。鸡群由本地母鸡自然孵化组成(88.88%);产蛋是季节性的,产蛋量少,几乎所有的孵化蛋都来自当地市场(84.5%)、周边小树林的个体采集和国内产卵。几内亚鸡属于食腐系统(66.66%),仅饲养。鸟类通常从少量谷物(玉米种子和小米)和家庭垃圾中受益。在8至12天龄的雏鸟中,雏鸟死亡率较高(80.55%)。大多数珍珠鸡饲养者(76%)不进行任何健康监测。技术和资金限制是发展珍珠鸡生产的主要挑战。生产者的组织、适当技术的传播以及扶持政策和私营部门的支持将改善总体生产业绩,从而合理利用这种本地遗传资源。
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引用次数: 3
Phenotypic and morphometric diversity of Indigenous Turkey (Meleagris Gallopavo) from Wilaya of Tlemcen, Northwest of Algeria 阿尔及利亚西北部特莱姆森Wilaya地区土生火鸡(Meleagris Gallopavo)表型和形态多样性
Pub Date : 2020-06-25 DOI: 10.46325/GABJ.V4I2.754
M. Mediouni, S. Sara, A. A. Ameur, S. Gaouar
In order to investigate the genetic and phenotypic variability of indigenous turkey in the North West of Algeria, a study was conducted around local poultry farms in the region of Tlemcen and performed on 78 Turkeys (27 males and 51 females) of adult age. Seventeen parameters were measured including 11 quantitative and 6 qualitative which are :body length (BL), wingspan (W), chest width (CW), chest size (CS), tarsal length (TL), thigh length (ThL), neck length (NL), spout length (SL), wing length (WL), width of the dewlap (WD), head length (HL). Taking in consideration plumage coloration, the following phenotypes were observed: Black (17%), White (65%), Red (18%). This work is considered as the first report about the phenotypic differentiation on indigenous turkey in Tlemcen. The measurements performed on these animals showed an important significant difference and a positive correlation between all the mentioned parameters thus the results of the hierarchical classification based on PCA shows three classes, where class 2 and class 3 were balanced with slight differences in (SL), (TL), (ThL), (CS), while class 1 showed remarkable differences which could be under implications of random selection and breeding programs
为了调查阿尔及利亚西北部本地火鸡的遗传和表型变异性,在特莱姆森地区的当地家禽养殖场周围进行了一项研究,对78只成年火鸡(27只公火鸡和51只母火鸡)进行了研究。测定了17个参数,其中定量参数11个,定性参数6个,分别为体长(BL)、翼展(W)、胸宽(CW)、胸围(CS)、跗骨长(TL)、大腿长(ThL)、颈长(NL)、口长(SL)、翼长(WL)、腋窝宽度(WD)、头长(HL)。考虑到羽毛的颜色,观察到以下表型:黑色(17%),白色(65%),红色(18%)。这项工作被认为是关于特莱姆森土生火鸡表型分化的首次报道。对这些动物的测量结果表明,上述参数之间存在重要的显著性差异和正相关关系,因此基于PCA的分层分类结果显示出3类,其中2类和3类平衡,(SL), (TL), (ThL), (CS)略有差异,而1类差异显著,这可能是随机选择和育种计划的影响
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引用次数: 2
Study of the phenotype and allele frequencies of the ABO and Rhesus blood group systems in Algeria. 阿尔及利亚ABO和恒河猴血型系统表型和等位基因频率的研究。
Pub Date : 2020-01-25 DOI: 10.46325/gabj.v5i1.171
Louiza Derouiche, Naima Belaribi, Hiba Akrour, Fatma Derouiche
The present work aims to study the distribution of blood groups in a simple random sampling and to present new national statistics on the phenotype and allele prevalence of the two ABO and Rhesus blood group systems. The study was carried out on a sample comprising 7549 individuals from the 48 Wilayas (Provinces) of Algeria. For this, a survey was carried out randomly, in hospitals, private laboratories, universities, university halls, Algiers Airport and social networks. The findings allowed stating that group O was found in about half of people with phenotypes (47.52%), group A was twice as high (30.14%) as group B (16.62%), and group AB exhibited the lowest frequency (5.72%). Moreover, a clear predominance of rhesus positive (Rh+) subjects (91.8%) was observed compared to rhesus negative (Rh-) subjects (8.1%) among the Algerian population. However, regarding the allele frequencies, it was found that the O allele was the most frequent, with a prevalence of 68.32%. As for the A allele, it came in the second position, with a frequency of 19.84%, and finally the B allele was the least frequent, with a frequency of 11.84%. The results obtained indicated that the Algerian population is heterogeneous; it is characterized by a high ethnic mix rate due to the migration of populations from all places (introduction of new parents) and to a very high percentage of consanguinity in certain communities due to consanguineous marriages.
目前的工作旨在研究血型的分布在一个简单的随机抽样,并提出新的国家统计表型和等位基因流行的两个ABO和恒河猴血型系统。这项研究的样本包括来自阿尔及利亚48个省的7549人。为此,在医院、私人实验室、大学、大学礼堂、阿尔及尔机场和社交网络中随机开展了一项调查。结果表明,O型的出现率约为一半(47.52%),A型的出现率为B型(16.62%)的两倍(30.14%),AB型的出现率最低(5.72%)。此外,在阿尔及利亚人群中,恒河猴阳性(Rh+)受试者(91.8%)明显高于恒河猴阴性(Rh-)受试者(8.1%)。而在等位基因频率方面,O等位基因频率最高,患病率为68.32%。A等位基因出现频率次之,为19.84%;B等位基因出现频率最低,为11.84%。结果表明,阿尔及利亚人口是异质的;它的特点是,由于各地人口的迁移(新父母的引入),种族混合率很高,由于近亲婚姻,某些社区的近亲比例非常高。
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引用次数: 0
Genetic Variations in Thomas’s Rope Squirrel (Funisciurus anerythrus) and Gambian Sun Squirrel (Heliosciurus gambianus) Ibadan, Nigeria, Using Allozyme Markers 尼日利亚伊巴丹托马斯绳松鼠(Funisciurus anerythrus)和冈比亚太阳松鼠(Heliosciurus gambianus)的等位酶标记遗传变异
Pub Date : 2020-01-25 DOI: 10.46325/gabj.v5i1.158
O. Coker, O. Osaiyuwu, O. M. Isong
Thomas’s Rope Squirrel (Funisciurus anerythrus) and Gambian Sun Squirrel (Heliosciurus gambianus) are two of the eight squirrel species found in Nigeria with overlapping habitats in Southwestern Nigeria. Squirrels are involved in pollination, seed dispersal, vectors of human and domestic animal diseases as well as crop and household pests. These species and the crucial ecosystem services they render are threatened by habitat destruction, human encroachment and the fact that they are being used as a source of protein in Nigeria. Their conservation would be enhanced by availability of accurate genetic information which are scarce for these species. This study seeks to provide baseline data on genetic variation at three allozyme loci in the two species in University of Ibadan, Ibadan, Nigeria. Twenty-nine individual squirrels including eighteen F. anerythrus species and eleven H. gambianus were used in this study. These were obtained from the wild within the University of Ibadan. Observed and expected heterozygosity (Ho and He), Hardy-Weinberg equilibrium (HWE), genetic distance between the species, and inbreeding coefficients were performed using POPGENE and Tools for Population Genetic Analyses (TFPGA). In F. anerythrus, Ho and He were 0.6092 and 0.5635, respectively, while, for H. gambianus, Ho and He were 0.6242 and 0.5745, respectively. There were no deviations from HWE in all the tested loci (p>0.05). Nei genetic distance between the species was 0.0070 and the populations showed fairly high level of outbreeding (FST = 0.0029 ± 0.0017). More robust genetic markers would be required to further ascertain the genetic status of the species.
托马斯绳松鼠(Funisciurus anerythrus)和冈比亚太阳松鼠(Heliosciurus gambianus)是在尼日利亚发现的八种松鼠中的两种,在尼日利亚西南部有重叠的栖息地。松鼠参与了授粉、种子传播、人类和家畜疾病以及作物和家庭害虫的传播。这些物种及其提供的重要生态系统服务受到栖息地破坏、人类入侵以及它们在尼日利亚被用作蛋白质来源的威胁。它们的保护将通过获得准确的遗传信息而得到加强,而这些信息对于这些物种来说是稀缺的。本研究旨在为尼日利亚伊巴丹大学的两个物种的三个同工酶位点的遗传变异提供基线数据。本研究以29只松鼠为研究对象,其中包括18种无角松鼠和11种甘比亚松鼠。这些是从伊巴丹大学的野外获得的。利用POPGENE和Tools for Population genetic Analyses (TFPGA)分析了观察和期望杂合度(Ho和He)、Hardy-Weinberg平衡(HWE)、种间遗传距离和近交系数。无毛螺旋体的Ho和He分别为0.6092和0.5635,冈边螺旋体的Ho和He分别为0.6242和0.5745。所有检测位点的HWE均无偏离(p>0.05)。种群间的内遗传距离为0.0070,种群间具有较高的远交水平(FST = 0.0029±0.0017)。需要更强大的遗传标记来进一步确定该物种的遗传状况。
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引用次数: 0
Phenotypic characterization of domestic cavies (Cavia porcellus) in the agricultural zone of Haut-Katanga, Democratic Republic of Congo 刚果民主共和国上加丹加农业区家养洞穴(Cavia porcellus)的表型特征
Pub Date : 2020-01-25 DOI: 10.46325/gabj.v5i1.160
K. D. Mwangomb, Ntata Cédric Ntemunyi, B. P. Bilolwa, Mwamba Innocent Tshimbangu, R. Ayagirwe, M. F. Kampemba, F. Meutchieye
This study was conducted with the aim of contributing to the characterization of zoo genetic resources for farm animals in the agricultural area of Haut Katanga. For this, 185 guinea pigs were sampled according to the snowball method from 117 households and whose weight parameter obtained from a balance and linear parameter obtained at the area of a measuring tape a description. The cavy was held immobile in a horizontal plane, and measurements were taken after locating landmarks on the surface of the animal's body. Qualitative parameters were collected by the visual method where the color of the coat was identified by direct observation under daylight (FAO, 2013; AU-IBAR, 2015). The main results showed that the location factor significantly influenced the majority of biometric characteristics while the sex factor had no significant effect on the measurements. The studied cavies had an angular conformation with the following average performances: weight 506.1 ± 149.9g; chest circumference 15.8 ± 3.0cm; body length 27.7 ± 3.2cm; thigh circumference 5.8 ± 0.9cm. Morphological characteristics indicate the presence of 6 main colors (white, cream, brown, grey, black and silver), from which several combinations are derived. The most frequent combinations are trichromic (White, black and brown) with 34.05%, followed by dichromic (White and brown: 18.38%). About 99.4% of the animals had black eyes. The hairs were soft (82.7%) and short (100%). PCA followed by an Ascending Hierarchical Classification (AHC) of these animals resulted in four genetic types; the first two types (type 1 and 2) have lower than average weights and resemble the guinea pigs of a (traditional) family farm, while the other two are semi-improved. Based on the coloring of the hair, these domestic cavies have a compound coat. These results show the existence of a great variability between the guinea pig populations as well as a diversity of rearing practices that requires monitoring for improvement, which constitutes an opportunity for genetic improvement of local guinea pigs.
本研究的目的是为上加丹加农业区农场动物动物园遗传资源的特征做出贡献。为此,根据滚雪球法从117户家庭中抽取185只豚鼠,其体重参数由天平获得,线性参数在卷尺面积处获得。将豚鼠固定在一个水平面上,并在动物身体表面定位地标后进行测量。定性参数通过目视法收集,其中在日光下直接观察确定被毛的颜色(FAO, 2013;AU-IBAR, 2015)。主要结果表明,地理位置因素对大部分生物特征有显著影响,而性别因素对测量结果无显著影响。所研究的豚鼠呈角状构象,平均性能为:体重506.1±149.9g;胸围15.8±3.0cm;体长27.7±3.2cm;大腿围5.8±0.9cm。形态特征表明存在6种主要颜色(白色,奶油色,棕色,灰色,黑色和银色),从中衍生出几种组合。最常见的组合是三色(白色、黑色和棕色),占34.05%,其次是二色(白色和棕色:18.38%)。大约99.4%的动物眼睛是黑色的。毛发柔软(82.7%),短(100%)。主成分分析(PCA)和层次上升分类(AHC)对这些动物进行了四种遗传类型;前两种类型(1型和2型)的体重低于平均水平,与(传统)家庭农场的豚鼠相似,而另外两种是半改良的。根据毛发的颜色,这些家养的洞穴有一层复合的皮毛。这些结果表明,天竺鼠种群之间存在很大差异,饲养方法也存在多样性,需要进行监测以进行改进,这为当地天竺鼠的遗传改进提供了机会。
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引用次数: 0
Roles of animal trophies in museums on conservation practices: case study of Palace museums of the west region of Cameroon 动物战利品在博物馆保护实践中的作用:喀麦隆西部地区宫殿博物馆的案例研究
Pub Date : 2020-01-25 DOI: 10.46325/gabj.v5i1.162
P.M.M Mfonden, F. Meutchieye
Palace museums harbour important animal remains (trophies), this is the case too with palace museums of the West region of Cameroon. Here we present a preliminary study into the animal-based collections of palace museums from a genetic conservation perspective. We surveyed 11 chiefdom palace museums in the West Region of Cameroon. We collected samples of these trophies. We extracted and purified DNA from these trophies using the DNeasy® Blood and Tissue Kit. Quantification and quality control were done using the agarose gel electrophoresis and spectrophotometric methods. From agarose gel electrophoresis, 64% of specimens have DNA that could be of good quality, 12% of specimens have contaminated DNA, while 12% of specimens have degraded DNA. Spectrophotometric determination reveals that 28% of the specimens has quantifiable DNA against 72% that was of poor DNA quantity. Specimens with good quality DNA could be used for further studies (for instance, DNA sequencing for wildlife traceability). In order to perpetuate the conservation role played by the tradition through museums, the administration has to regulate the acquisition and use of trophies in traditional and cultural events in the forest law actually under revision. Museums have to ensure compliance with the criteria of acquisition and management of wildlife trophies
故宫博物院藏有重要的动物遗骸(战利品),喀麦隆西部地区的故宫博物院也是如此。本文从遗传保护的角度对故宫博物院的动物馆藏进行了初步研究。我们调查了喀麦隆西部地区的11个酋长宫殿博物馆。我们收集了这些奖杯的样本。我们使用dnasy®血液和组织试剂盒从这些奖杯中提取和纯化DNA。采用琼脂糖凝胶电泳和分光光度法进行定量和质量控制。琼脂糖凝胶电泳显示,64%的标本具有可能质量良好的DNA, 12%的标本具有受污染的DNA, 12%的标本具有降解的DNA。分光光度法测定显示,28%的标本具有可量化的DNA,而72%的标本DNA含量较差。具有高质量DNA的标本可用于进一步研究(例如,用于野生动物可追溯性的DNA测序)。为了通过博物馆使传统所发挥的保护作用永久化,行政当局必须在实际正在修订的森林法中对传统和文化活动中战利品的获取和使用进行管制。博物馆必须确保符合获取和管理野生动物战利品的标准
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