Machi Tanaka, H. Ooyama, Mieko Yokozeki, M. Otsuki, H. Moromizato, K. Ooyama, Norifumi Takagi, Shuntaro Miyazaki, S. Fujimori
There are various lifestyle - related diseases including gout and hyperuricemia that can be improved by reducing alcohol intake. Many treatment guidelines set appropriate volumes of alcohol intake, however, specific educational methods are not mentioned. The The Use Disorders Identification Test （ AUDIT ） that consists of 10 questions to identify drinking - related problems. They proposed an educational method that aims to improve recognition of the necessity of reducing alcohol intake by utilizing AUDIT and repeated support as brief intervention to achieve this goal. absolute the and appropriate alcohol 20 g individual goals to reduce alcohol consumption. interviews were with 3 patients, revealing that all of had achieved their goals and showed decreases in the γ GTP value. The subject group of this study was very small and support for reducing alcohol intake was limited. Daily educational methods to tackle drinking problems will be revised based on the results.
{"title":"Education of drinking habit for the patients with gout and hyperuricemia","authors":"Machi Tanaka, H. Ooyama, Mieko Yokozeki, M. Otsuki, H. Moromizato, K. Ooyama, Norifumi Takagi, Shuntaro Miyazaki, S. Fujimori","doi":"10.6032/GNAM.42.15","DOIUrl":"https://doi.org/10.6032/GNAM.42.15","url":null,"abstract":"There are various lifestyle - related diseases including gout and hyperuricemia that can be improved by reducing alcohol intake. Many treatment guidelines set appropriate volumes of alcohol intake, however, specific educational methods are not mentioned. The The Use Disorders Identification Test （ AUDIT ） that consists of 10 questions to identify drinking - related problems. They proposed an educational method that aims to improve recognition of the necessity of reducing alcohol intake by utilizing AUDIT and repeated support as brief intervention to achieve this goal. absolute the and appropriate alcohol 20 g individual goals to reduce alcohol consumption. interviews were with 3 patients, revealing that all of had achieved their goals and showed decreases in the γ GTP value. The subject group of this study was very small and support for reducing alcohol intake was limited. Daily educational methods to tackle drinking problems will be revised based on the results.","PeriodicalId":12746,"journal":{"name":"GOUT AND NUCLEIC ACID METABOLISM","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84152048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Masafumi Kurajoh, S. Okuno, Jin Miyawaki, H. Okazaki, S. Shoji, T. Yamakawa, E. Ishimura, Masaaki Inaba
{"title":"血液透析患者においてプリノール服用は骨密度と関連する","authors":"Masafumi Kurajoh, S. Okuno, Jin Miyawaki, H. Okazaki, S. Shoji, T. Yamakawa, E. Ishimura, Masaaki Inaba","doi":"10.6032/gnam.42.135","DOIUrl":"https://doi.org/10.6032/gnam.42.135","url":null,"abstract":"","PeriodicalId":12746,"journal":{"name":"GOUT AND NUCLEIC ACID METABOLISM","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78186576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Required conditions for cilnidipine-induced decrease in serum uric acid in hypertensive patients with nephropathy","authors":"M. Saita","doi":"10.6032/gnam.42.41","DOIUrl":"https://doi.org/10.6032/gnam.42.41","url":null,"abstract":"","PeriodicalId":12746,"journal":{"name":"GOUT AND NUCLEIC ACID METABOLISM","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90475086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tomoyuki Yamasaki, S. Marukawa, Toru Oka, M. Mukai
{"title":"人間ドック検診例における血清尿酸値分布と低尿酸血症の解析","authors":"Tomoyuki Yamasaki, S. Marukawa, Toru Oka, M. Mukai","doi":"10.6032/gnam.42.141","DOIUrl":"https://doi.org/10.6032/gnam.42.141","url":null,"abstract":"","PeriodicalId":12746,"journal":{"name":"GOUT AND NUCLEIC ACID METABOLISM","volume":"223 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75685882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Kamatani, K. Furihata, A. Taniguchi, T. Fukuuchi, N. Yamaoka, K. Kaneko, H. Kanno
Objective: To examine whether the supplementation of inosine augments ATP in vitro in human erythrocytes incubated in saline. Methods : Peripheral blood was drawn from each of three subjects, i.e. one healthy male and two males with thalassemia and hemoglobinopathy. After washing the erythrocytes in saline, they were suspended in saline to which inosine was added to final concentrations of 0, 0.5 and 2.5 mM. The suspension was incubated at 37 °C for 1 or 3 hours, and 0.5 ml ice cold 8% perchloric acid was added to the 0.5 ml erythrocyte-containing solution. After removing precipitates and perchloric acid, the supernatant was submitted to HPLC for the measurement of ATP. Results : Since the blood samples of the two subjects with thalassemia and hemoglobinopathy were transported from the clinics to the laboratory, ATP in the blood decreased considerably during the transportation. However, the reduction of ATP with time was observed in the erythrocytes in saline obtained from each of the three subjects during the incubation from 1 hour to 3 hours. In addition, dosedependent suppression of the decrease of ATP with inosine was observed in all the three cases at both 1 hour and 3 hour incubation times. Conclusions : Incubation of erythrocytes from a healthy subject and two thalassemia /hemoglobinopathy patients in saline at 37 °C resulted in time-dependent decreases of ATP. Supplementation of inosine to the solutions resulted in the suppression of the decreases of ATP in a dose-dependent manner. Background ATP(adenosine triphosphate)is the most important compound for storing energy in living organisms and supplying it when needed. It is considered that ATP reduction is related to the pathology of various diseases. An example is the hemolytic anemia caused by glycolytic enzyme deficiencies such as hexokinase deficiency1), pyruvate kinase deficiency2), glucose phosphate isomerase deficiency3), phosphofructokinase deficiency4)and phosphoglycerate kinase deficiency5). Since mature erythrocytes lack mitochondria, they are heavily dependent on the anaerobic generation of ATP during glycolysis for nearly all of their 受付:2018年4月2日,受理:2018年4月25日 1) Tsukuba International Clinical Pharmacology Clinic, Tsukuba, Japan 2) StaGen Co. Ltd, Tokyo, Japan 3) P-One Clinic, Keikokai Medical Corporation, Hachioji, Tokyo, Japan 4) Institute of Rheumatology, Tokyo Women's Medical University, Tokyo, Japan 5) Laboratory of Biomedical and Analytical Sciences, Faculty of Pharma-Science, Teikyo University, Tokyo, Japan 6) Department of Transfusion Medicine and Cell Processing, Tokyo Women's Medical University, Tokyo, Japan
{"title":"In vitro enhancement of ATP in human erythrocytes from a healthy subject and two patients with thalassemia and hemoglobinopathy.","authors":"N. Kamatani, K. Furihata, A. Taniguchi, T. Fukuuchi, N. Yamaoka, K. Kaneko, H. Kanno","doi":"10.6032/GNAM.42.59","DOIUrl":"https://doi.org/10.6032/GNAM.42.59","url":null,"abstract":"Objective: To examine whether the supplementation of inosine augments ATP in vitro in human erythrocytes incubated in saline. Methods : Peripheral blood was drawn from each of three subjects, i.e. one healthy male and two males with thalassemia and hemoglobinopathy. After washing the erythrocytes in saline, they were suspended in saline to which inosine was added to final concentrations of 0, 0.5 and 2.5 mM. The suspension was incubated at 37 °C for 1 or 3 hours, and 0.5 ml ice cold 8% perchloric acid was added to the 0.5 ml erythrocyte-containing solution. After removing precipitates and perchloric acid, the supernatant was submitted to HPLC for the measurement of ATP. Results : Since the blood samples of the two subjects with thalassemia and hemoglobinopathy were transported from the clinics to the laboratory, ATP in the blood decreased considerably during the transportation. However, the reduction of ATP with time was observed in the erythrocytes in saline obtained from each of the three subjects during the incubation from 1 hour to 3 hours. In addition, dosedependent suppression of the decrease of ATP with inosine was observed in all the three cases at both 1 hour and 3 hour incubation times. Conclusions : Incubation of erythrocytes from a healthy subject and two thalassemia /hemoglobinopathy patients in saline at 37 °C resulted in time-dependent decreases of ATP. Supplementation of inosine to the solutions resulted in the suppression of the decreases of ATP in a dose-dependent manner. Background ATP(adenosine triphosphate)is the most important compound for storing energy in living organisms and supplying it when needed. It is considered that ATP reduction is related to the pathology of various diseases. An example is the hemolytic anemia caused by glycolytic enzyme deficiencies such as hexokinase deficiency1), pyruvate kinase deficiency2), glucose phosphate isomerase deficiency3), phosphofructokinase deficiency4)and phosphoglycerate kinase deficiency5). Since mature erythrocytes lack mitochondria, they are heavily dependent on the anaerobic generation of ATP during glycolysis for nearly all of their 受付:2018年4月2日,受理:2018年4月25日 1) Tsukuba International Clinical Pharmacology Clinic, Tsukuba, Japan 2) StaGen Co. Ltd, Tokyo, Japan 3) P-One Clinic, Keikokai Medical Corporation, Hachioji, Tokyo, Japan 4) Institute of Rheumatology, Tokyo Women's Medical University, Tokyo, Japan 5) Laboratory of Biomedical and Analytical Sciences, Faculty of Pharma-Science, Teikyo University, Tokyo, Japan 6) Department of Transfusion Medicine and Cell Processing, Tokyo Women's Medical University, Tokyo, Japan","PeriodicalId":12746,"journal":{"name":"GOUT AND NUCLEIC ACID METABOLISM","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89272240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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