Hikota Osawa, Atsuo Taniguchi, C. Sekita, H. Kaneko, Naomi Ichikawa, Y. Seto, H. Yamanaka, Yasukazu Yamada
{"title":"HPRT1欠損症9例の遺伝子解析","authors":"Hikota Osawa, Atsuo Taniguchi, C. Sekita, H. Kaneko, Naomi Ichikawa, Y. Seto, H. Yamanaka, Yasukazu Yamada","doi":"10.6032/gnam.42.123","DOIUrl":"https://doi.org/10.6032/gnam.42.123","url":null,"abstract":"","PeriodicalId":12746,"journal":{"name":"GOUT AND NUCLEIC ACID METABOLISM","volume":"113 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87633673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Kamatani, K. Furihata, A. Taniguchi, T. Fukuuchi, N. Yamaoka, K. Kaneko, H. Kanno
Objective: To examine whether the supplementation of inosine augments ATP in vitro in human erythrocytes incubated in saline. Methods : Peripheral blood was drawn from each of three subjects, i.e. one healthy male and two males with thalassemia and hemoglobinopathy. After washing the erythrocytes in saline, they were suspended in saline to which inosine was added to final concentrations of 0, 0.5 and 2.5 mM. The suspension was incubated at 37 °C for 1 or 3 hours, and 0.5 ml ice cold 8% perchloric acid was added to the 0.5 ml erythrocyte-containing solution. After removing precipitates and perchloric acid, the supernatant was submitted to HPLC for the measurement of ATP. Results : Since the blood samples of the two subjects with thalassemia and hemoglobinopathy were transported from the clinics to the laboratory, ATP in the blood decreased considerably during the transportation. However, the reduction of ATP with time was observed in the erythrocytes in saline obtained from each of the three subjects during the incubation from 1 hour to 3 hours. In addition, dosedependent suppression of the decrease of ATP with inosine was observed in all the three cases at both 1 hour and 3 hour incubation times. Conclusions : Incubation of erythrocytes from a healthy subject and two thalassemia /hemoglobinopathy patients in saline at 37 °C resulted in time-dependent decreases of ATP. Supplementation of inosine to the solutions resulted in the suppression of the decreases of ATP in a dose-dependent manner. Background ATP(adenosine triphosphate)is the most important compound for storing energy in living organisms and supplying it when needed. It is considered that ATP reduction is related to the pathology of various diseases. An example is the hemolytic anemia caused by glycolytic enzyme deficiencies such as hexokinase deficiency1), pyruvate kinase deficiency2), glucose phosphate isomerase deficiency3), phosphofructokinase deficiency4)and phosphoglycerate kinase deficiency5). Since mature erythrocytes lack mitochondria, they are heavily dependent on the anaerobic generation of ATP during glycolysis for nearly all of their 受付:2018年4月2日,受理:2018年4月25日 1) Tsukuba International Clinical Pharmacology Clinic, Tsukuba, Japan 2) StaGen Co. Ltd, Tokyo, Japan 3) P-One Clinic, Keikokai Medical Corporation, Hachioji, Tokyo, Japan 4) Institute of Rheumatology, Tokyo Women's Medical University, Tokyo, Japan 5) Laboratory of Biomedical and Analytical Sciences, Faculty of Pharma-Science, Teikyo University, Tokyo, Japan 6) Department of Transfusion Medicine and Cell Processing, Tokyo Women's Medical University, Tokyo, Japan
{"title":"In vitro enhancement of ATP in human erythrocytes from a healthy subject and two patients with thalassemia and hemoglobinopathy.","authors":"N. Kamatani, K. Furihata, A. Taniguchi, T. Fukuuchi, N. Yamaoka, K. Kaneko, H. Kanno","doi":"10.6032/GNAM.42.59","DOIUrl":"https://doi.org/10.6032/GNAM.42.59","url":null,"abstract":"Objective: To examine whether the supplementation of inosine augments ATP in vitro in human erythrocytes incubated in saline. Methods : Peripheral blood was drawn from each of three subjects, i.e. one healthy male and two males with thalassemia and hemoglobinopathy. After washing the erythrocytes in saline, they were suspended in saline to which inosine was added to final concentrations of 0, 0.5 and 2.5 mM. The suspension was incubated at 37 °C for 1 or 3 hours, and 0.5 ml ice cold 8% perchloric acid was added to the 0.5 ml erythrocyte-containing solution. After removing precipitates and perchloric acid, the supernatant was submitted to HPLC for the measurement of ATP. Results : Since the blood samples of the two subjects with thalassemia and hemoglobinopathy were transported from the clinics to the laboratory, ATP in the blood decreased considerably during the transportation. However, the reduction of ATP with time was observed in the erythrocytes in saline obtained from each of the three subjects during the incubation from 1 hour to 3 hours. In addition, dosedependent suppression of the decrease of ATP with inosine was observed in all the three cases at both 1 hour and 3 hour incubation times. Conclusions : Incubation of erythrocytes from a healthy subject and two thalassemia /hemoglobinopathy patients in saline at 37 °C resulted in time-dependent decreases of ATP. Supplementation of inosine to the solutions resulted in the suppression of the decreases of ATP in a dose-dependent manner. Background ATP(adenosine triphosphate)is the most important compound for storing energy in living organisms and supplying it when needed. It is considered that ATP reduction is related to the pathology of various diseases. An example is the hemolytic anemia caused by glycolytic enzyme deficiencies such as hexokinase deficiency1), pyruvate kinase deficiency2), glucose phosphate isomerase deficiency3), phosphofructokinase deficiency4)and phosphoglycerate kinase deficiency5). Since mature erythrocytes lack mitochondria, they are heavily dependent on the anaerobic generation of ATP during glycolysis for nearly all of their 受付:2018年4月2日,受理:2018年4月25日 1) Tsukuba International Clinical Pharmacology Clinic, Tsukuba, Japan 2) StaGen Co. Ltd, Tokyo, Japan 3) P-One Clinic, Keikokai Medical Corporation, Hachioji, Tokyo, Japan 4) Institute of Rheumatology, Tokyo Women's Medical University, Tokyo, Japan 5) Laboratory of Biomedical and Analytical Sciences, Faculty of Pharma-Science, Teikyo University, Tokyo, Japan 6) Department of Transfusion Medicine and Cell Processing, Tokyo Women's Medical University, Tokyo, Japan","PeriodicalId":12746,"journal":{"name":"GOUT AND NUCLEIC ACID METABOLISM","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89272240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Nakayama, H. Matsuo, A. Ohtahara, K. Ogino, M. Hakoda, T. Hamada, M. Hosoyamada, Satoshi Yamaguchi, I. Hisatome, K. Ichida, N. Shinomiya
{"title":"Development of“ Clinical Practice Guideline for Renal Hypouricemia” (1st edition)","authors":"A. Nakayama, H. Matsuo, A. Ohtahara, K. Ogino, M. Hakoda, T. Hamada, M. Hosoyamada, Satoshi Yamaguchi, I. Hisatome, K. Ichida, N. Shinomiya","doi":"10.6032/GNAM.42.1","DOIUrl":"https://doi.org/10.6032/GNAM.42.1","url":null,"abstract":"","PeriodicalId":12746,"journal":{"name":"GOUT AND NUCLEIC ACID METABOLISM","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87495293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"小児ウイルス腸炎における高尿酸血症―尿酸排泄動態からの検討―","authors":"Atsushi Hayashi, A. Ohtahara, E. Mizuta","doi":"10.6032/gnam.42.127","DOIUrl":"https://doi.org/10.6032/gnam.42.127","url":null,"abstract":"","PeriodicalId":12746,"journal":{"name":"GOUT AND NUCLEIC ACID METABOLISM","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85419727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Masafumi Kurajoh, Y. Moriwaki, H. Koyama, H. Tsuboi, H. Matsuda, Yamada Naruomi, C. Saito, H. Kano, Y. Asami, Tetsuya Yamamoto
Objective: Ingestion of yogurt containing Lactobacillus gasseri PA-3(PA-3, Accession No : NITE BP-224) (PA-3Y)has been shown to reduce serum uric acid(SUA)levels by interfering with the intestinal absorption of food-derived purines in animal studies. To confirm this mechanism in humans, the ability of PA-3 intake to alleviate purine ingestion-induced increases in SUA levels was analyzed. Research Methods and Procedures: In this randomized, double-blind, placebo-controlled crossover study, 16 healthy adults were randomized to groups ingesting of 112 g of PA-3Y or yogurt without PA-3(control yogurt)in addition to standardized meals for 3 days. Purine-loading tests, in which subjects ingested 112 g of PA-3Y or control yogurt followed immediately by 498 mg of a mixture of purine nucleotides, were performed on the fourth day of each test period. Blood and urine samples were collected before and after the purine-loading tests. Results : The increase in the SUA concentration from the baseline was significantly lower following the ingestion of PA-3Y than of control yogurt alone, especially at 30(P=0.033)and 60(P=0.028)minutes. In addition, the area under the curve for the increase in the SUA concentration from the baseline to 150 minutes was also significantly lower (P=0.041)in the PA-3Y than in the control yogurt group. However, urinary and fractional excretions of uric acid were not different between the two groups. Conclusion : The ingestion of PA-3 before purine intake alleviates the increase in SUA levels, probably by reducing purine absorption in the intestine, and not by enhancing urinary excretion of uric acid. Introduction Hyperuricemia is defined as a serum uric acid (SUA)concentration over 7.0 mg dL1. Persistent hyperuricemia can lead to the deposition of urate crystals in the joints and kidneys, resulting in acute arthritis or kidney injury such as nephrolithiasis and nephropathy13). Hyperuricemia has also been associated Masafumi Kurajoh1, 2) Yuji Moriwaki1) Hidenori Koyama1) Hiroshi Tsuboi3) Hirotaka Matsuda3) Naruomi Yamada3) Chizuru Saito3) Hiroshi Kano3) Yukio Asami3) Tetsuya Yamamoto1) Yogurt containing Lactobacillus gasseri PA-3 alleviates increases in serum uric acid concentration induced by purine ingestion: a randomized, double-blind, placebo-controlled study 受付:2017年11月10日,受理:2018年3月2日 1) Division of Diabetes, Endocrinology and Metabolism, Department of Internal Medicine, Hyogo College of Medicine 2) Department of Metabolism, Endocrinology, and Molecular Medicine, Osaka City University Graduate School of Medicine 3) Food Science Research Laboratories, Meiji Co., LTD.
{"title":"Yogurt containing Lactobacillus gasseri PA-3 alleviates increases in serum uric acid concentration induced by purine ingestion: a randomized, double-blind, placebo-controlled study","authors":"Masafumi Kurajoh, Y. Moriwaki, H. Koyama, H. Tsuboi, H. Matsuda, Yamada Naruomi, C. Saito, H. Kano, Y. Asami, Tetsuya Yamamoto","doi":"10.6032/GNAM.42.31","DOIUrl":"https://doi.org/10.6032/GNAM.42.31","url":null,"abstract":"Objective: Ingestion of yogurt containing Lactobacillus gasseri PA-3(PA-3, Accession No : NITE BP-224) (PA-3Y)has been shown to reduce serum uric acid(SUA)levels by interfering with the intestinal absorption of food-derived purines in animal studies. To confirm this mechanism in humans, the ability of PA-3 intake to alleviate purine ingestion-induced increases in SUA levels was analyzed. Research Methods and Procedures: In this randomized, double-blind, placebo-controlled crossover study, 16 healthy adults were randomized to groups ingesting of 112 g of PA-3Y or yogurt without PA-3(control yogurt)in addition to standardized meals for 3 days. Purine-loading tests, in which subjects ingested 112 g of PA-3Y or control yogurt followed immediately by 498 mg of a mixture of purine nucleotides, were performed on the fourth day of each test period. Blood and urine samples were collected before and after the purine-loading tests. Results : The increase in the SUA concentration from the baseline was significantly lower following the ingestion of PA-3Y than of control yogurt alone, especially at 30(P=0.033)and 60(P=0.028)minutes. In addition, the area under the curve for the increase in the SUA concentration from the baseline to 150 minutes was also significantly lower (P=0.041)in the PA-3Y than in the control yogurt group. However, urinary and fractional excretions of uric acid were not different between the two groups. Conclusion : The ingestion of PA-3 before purine intake alleviates the increase in SUA levels, probably by reducing purine absorption in the intestine, and not by enhancing urinary excretion of uric acid. Introduction Hyperuricemia is defined as a serum uric acid (SUA)concentration over 7.0 mg dL1. Persistent hyperuricemia can lead to the deposition of urate crystals in the joints and kidneys, resulting in acute arthritis or kidney injury such as nephrolithiasis and nephropathy13). Hyperuricemia has also been associated Masafumi Kurajoh1, 2) Yuji Moriwaki1) Hidenori Koyama1) Hiroshi Tsuboi3) Hirotaka Matsuda3) Naruomi Yamada3) Chizuru Saito3) Hiroshi Kano3) Yukio Asami3) Tetsuya Yamamoto1) Yogurt containing Lactobacillus gasseri PA-3 alleviates increases in serum uric acid concentration induced by purine ingestion: a randomized, double-blind, placebo-controlled study 受付:2017年11月10日,受理:2018年3月2日 1) Division of Diabetes, Endocrinology and Metabolism, Department of Internal Medicine, Hyogo College of Medicine 2) Department of Metabolism, Endocrinology, and Molecular Medicine, Osaka City University Graduate School of Medicine 3) Food Science Research Laboratories, Meiji Co., LTD.","PeriodicalId":12746,"journal":{"name":"GOUT AND NUCLEIC ACID METABOLISM","volume":"77 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89534491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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