Two novel HLA-С alleles HLA-C*07:1153 and -C*07:1154 alleles detected during routine next generation sequencing.
Two novel HLA-С alleles HLA-C*07:1153 and -C*07:1154 alleles detected during routine next generation sequencing.
The novel allele HLA-DPB1*1327:01 has a non-synonymous mutation difference compared with HLA-DPB1*04:01:01.
Four novel HLA alleles HLA-A*02:01:222, -B*07:02:104, -C*07:02:156 and -DQB1*06:03:51 alleles detected during routine next generation sequencing.
Full genomic sequence shows HLA-B*40:186:02 differs from HLA-B*40:186:01 by a cytosine substitution in exon 2.
HLA-B*40:523 differs from HLA-B*40:92 in codon 167 in exon 3.
HLA-B*51:377N differs from HLA-B*51:01:01:01 in codon 13 in exon 2.
HLA-B*37:107 differs from HLA-B*37:01:01:01 in codon 25 in exon 2.
A subpopulation of NK cells with distinctive phenotype and function differentiates and expands specifically in response to infection by human cytomegalovirus (HCMV). A hallmark of these adaptive NK cells is their increased expression levels of the activating CD94/NKG2C receptor for HLA-E, and lack of expression of its inhibitory homologue CD94/NKG2A. Their frequency is highly variable in HCMV+ individuals, and the basis for such differences is only partially understood. Here, we explore the possible influence of sequence polymorphism of the NKG2C (or KLRC2) gene on the expansion of NKG2C+NKG2A− NK cells in healthy HCMV-seropositive donors. Our results show a significant association of greater proportions of adaptive NK cells with allele NKG2C*02. This is defined by two amino acid substitutions in comparison with the most prevalent allele, NKG2C*01, and associates with additional sequence polymorphisms in noncoding regions. Furthermore, we demonstrate consistently higher mRNA levels of NKG2C*02 in heterozygous individuals co-expressing this allele in combination with NKG2C*01 or *03. This predominance is independent of polymorphisms in the promoter and 3′ UTRs and is appreciated also in HCMV-seronegative donors. In summary, although additional factors are most likely implicated in the variable expansion of NKG2C+NKG2A− NK cells in response to HCMV, our results demonstrate that host immunogenetics, in particular NKG2C diversity, influences the magnitude of such response.
The novel HLA-B*35:20:03 allele differs from B*35:20:01 by a change of C→A in exon 4.
HLA-E*01:151 differs from HLA-E*01:03:01:01 by a single nucleotide substitution at position 890 C>T in exon 5.
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