HLA-G*01:63 differs from HLA-G*01:01:01:01 by a single nonsynonymous nucleotide substitution in codon 259 of exon 4.
HLA-G*01:63 differs from HLA-G*01:01:01:01 by a single nonsynonymous nucleotide substitution in codon 259 of exon 4.
HLA-DPB1*1462:01 differs from HLA-DPB1*05:01:01:01 by one single nucleotide substitution in Exon 3.
Description of the novel HLA-DRB1*16:84 allele.
HLA-DRB1*09:64 differs from HLA-DRB1*09:01:02:05 by a single nucleotide substitution in exon 3.
HLA-DPB1*1784:01 differs from HLA-DPB1*514:01 by one nucleotide substitution in codon 57 in exon 2.
The autoimmune regulator (AIRE) gene deficiency in humans causes the autoimmune polyglandular syndrome type 1 (APS-1). AIRE contributes to self-tolerance and prevents autoimmunity via the regulation of tissue-restricted antigen (TRA) expression in medullary thymic epithelial cells (mTECs). Using a murine Aire-deficient mTEC line (Aire−/−), we previously reported that Aire regulates cell adhesion genes, affecting homotypic mTEC/mTEC and heterotypic mTEC/thymocyte interaction in vitro. Transcriptional analysis comparing AireWT with Aire−/− mTECs indicates that Aire modulates antigen processing and presentation genes. Aire−/− mTECs, but not AireWT, exhibited delayed expression of MHC II and CD74 molecules, suggesting that Aire influences the MHC II presentation pathway. However, AireWT and Aire−/− mTECs did not directly present the OVA323–339 peptide to OT-II TCR transgenic single-positive CD4+ T cells. When cocultured with splenocytes and dendritic cells, both mTEC types induce T cell activation and present the OVA323–339 peptide to OT-II TCR transgenic T cells. To further understand Aire's role in MHC II antigen processing/presentation, we evaluated the expression of the MHC II transactivator (Ciita), IFN-γ receptors and IFN-γ-induced GTPases, as well as lysosome and cathepsin L activities. After IFN-γ treatment, Aire−/− mTECs, but not AireWT, delayed the expression of Ciita. Our results suggest that Aire controls some steps of the MHC II antigen processing/presentation-related proteins.
HLA-A*02:1259 differs from HLA-A*02:07:01:01 by two non-synonymous nucleotide changes in exon 3.
HLA-DQB1*06:02:01:42 differs from HLA-DQB1*06:02:01:01 by one nucleotide change in intron 1.
HLA-DPA1*02:164 differs from HLA-DPA1*02:02:02:01 by a non-synonymous nucleotide substitution in exon 3 and an intronic nucleotide substitution in intron 1.
The HLA-B*46:106 allele differs from HLA-B*46:01:01:01 by one nucleotide substitution in codon 66 in exon 2.
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：