A. C. Monteleone-Cassiano, R. D. Pinto, P. Ferreirinha, U. G. Cipriano, A. F. Gembre, V. L. D. Bonato, G. A. Passos, N. L. Alves, E. A. Donadi
The autoimmune regulator (AIRE) gene deficiency in humans causes the autoimmune polyglandular syndrome type 1 (APS-1). AIRE contributes to self-tolerance and prevents autoimmunity via the regulation of tissue-restricted antigen (TRA) expression in medullary thymic epithelial cells (mTECs). Using a murine Aire-deficient mTEC line (Aire−/−), we previously reported that Aire regulates cell adhesion genes, affecting homotypic mTEC/mTEC and heterotypic mTEC/thymocyte interaction in vitro. Transcriptional analysis comparing AireWT with Aire−/− mTECs indicates that Aire modulates antigen processing and presentation genes. Aire−/− mTECs, but not AireWT, exhibited delayed expression of MHC II and CD74 molecules, suggesting that Aire influences the MHC II presentation pathway. However, AireWT and Aire−/− mTECs did not directly present the OVA323–339 peptide to OT-II TCR transgenic single-positive CD4+ T cells. When cocultured with splenocytes and dendritic cells, both mTEC types induce T cell activation and present the OVA323–339 peptide to OT-II TCR transgenic T cells. To further understand Aire's role in MHC II antigen processing/presentation, we evaluated the expression of the MHC II transactivator (Ciita), IFN-γ receptors and IFN-γ-induced GTPases, as well as lysosome and cathepsin L activities. After IFN-γ treatment, Aire−/− mTECs, but not AireWT, delayed the expression of Ciita. Our results suggest that Aire controls some steps of the MHC II antigen processing/presentation-related proteins.
{"title":"The Role of the Autoimmune Regulator Gene in the Control of MHC II Antigen-Processing and Presentation by Medullary Thymic Epithelial Cells","authors":"A. C. Monteleone-Cassiano, R. D. Pinto, P. Ferreirinha, U. G. Cipriano, A. F. Gembre, V. L. D. Bonato, G. A. Passos, N. L. Alves, E. A. Donadi","doi":"10.1111/tan.70516","DOIUrl":"10.1111/tan.70516","url":null,"abstract":"<p>The autoimmune regulator (<i>AIRE</i>) gene deficiency in humans causes the autoimmune polyglandular syndrome type 1 (APS-1). <i>AIRE</i> contributes to self-tolerance and prevents autoimmunity via the regulation of tissue-restricted antigen (TRA) expression in medullary thymic epithelial cells (mTECs). Using a murine <i>Aire</i>-deficient mTEC line (<i>Aire</i><sup>−/−</sup>), we previously reported that <i>Aire</i> regulates cell adhesion genes, affecting homotypic mTEC/mTEC and heterotypic mTEC/thymocyte interaction in vitro. Transcriptional analysis comparing <i>Aire</i><sup><i>WT</i></sup> with <i>Aire</i><sup><i>−/−</i></sup> mTECs indicates that <i>Aire</i> modulates antigen processing and presentation genes. <i>Aire</i><sup>−/−</sup> mTECs, but not <i>Aire</i><sup><i>WT</i></sup>, exhibited delayed expression of MHC II and CD74 molecules, suggesting that <i>Aire</i> influences the MHC II presentation pathway. However, <i>Aire</i><sup><i>WT</i></sup> and <i>Aire</i><sup>−/−</sup> mTECs did not directly present the OVA<sup>323–339</sup> peptide to OT-II TCR transgenic single-positive CD4+ T cells. When cocultured with splenocytes and dendritic cells, both mTEC types induce T cell activation and present the OVA<sup>323–339</sup> peptide to OT-II TCR transgenic T cells. To further understand <i>Aire'</i>s role in MHC II antigen processing/presentation, we evaluated the expression of the MHC II transactivator (<i>Ciita</i>), IFN-γ receptors and IFN-γ-induced GTPases, as well as lysosome and cathepsin L activities. After IFN-γ treatment, <i>Aire</i><sup>−/−</sup> mTECs, but not <i>Aire</i><sup><i>WT</i></sup>, delayed the expression of <i>Ciita</i>. Our results suggest <i>that Aire</i> controls some steps of the MHC II antigen processing/presentation-related proteins.</p>","PeriodicalId":13172,"journal":{"name":"HLA","volume":"106 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12722109/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145810076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Novel HLA-A*02:1259 Allele, Identified by Sanger Dideoxy Nucleotide Sequencing in a Chinese Individual","authors":"Yao-gong Wu, Ming-ming Wang, Yi-bing Yang, Pei-pei Ding, Heng Mei","doi":"10.1111/tan.70498","DOIUrl":"10.1111/tan.70498","url":null,"abstract":"<div>\u0000 \u0000 <p><i>HLA-A*02:1259</i> differs from <i>HLA-A*02:07:01:01</i> by two non-synonymous nucleotide changes in exon 3.</p>\u0000 </div>","PeriodicalId":13172,"journal":{"name":"HLA","volume":"106 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145810037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A New Allele HLA-DQB1*06:02:01:42 Identified by HLA-Targeted Third-Generation Sequencing in a Chinese Individual","authors":"Jindou Wang, Guiliang Shen, Xinyu Wang, Peng Gao, Yuanli Zhao","doi":"10.1111/tan.70517","DOIUrl":"10.1111/tan.70517","url":null,"abstract":"<div>\u0000 \u0000 <p><i>HLA-DQB1*06:02:01:42</i> differs from <i>HLA-DQB1*06:02:01:01</i> by one nucleotide change in intron 1.</p>\u0000 </div>","PeriodicalId":13172,"journal":{"name":"HLA","volume":"106 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145774585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HLA-DPA1*02:164 differs from HLA-DPA1*02:02:02:01 by a non-synonymous nucleotide substitution in exon 3 and an intronic nucleotide substitution in intron 1.
{"title":"Characterisation of the Novel HLA-DPA1*02:164 Allele Identified by Next Generation Sequencing","authors":"John Jeongseok Yang, Sohyeon Eom, Eun-Suk Kang","doi":"10.1111/tan.70514","DOIUrl":"10.1111/tan.70514","url":null,"abstract":"<div>\u0000 \u0000 <p><i>HLA-DPA1*02:164</i> differs from <i>HLA-DPA1*02:02:02:01</i> by a non-synonymous nucleotide substitution in exon 3 and an intronic nucleotide substitution in intron 1.</p>\u0000 </div>","PeriodicalId":13172,"journal":{"name":"HLA","volume":"106 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145774562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Novel HLA-B*46:106 Allele, Identified by Sanger Dideoxy Nucleotide Sequencing in a Chinese Individual","authors":"Jie Wang, Dong Mei Li, Yu Jie Wen, Yan Jun Jia, Tie Cheng Sun","doi":"10.1111/tan.70521","DOIUrl":"10.1111/tan.70521","url":null,"abstract":"<div>\u0000 \u0000 <p>The <i>HLA-B*46:106</i> allele differs from <i>HLA-B*46:01:01:01</i> by one nucleotide substitution in codon 66 in exon 2.</p>\u0000 </div>","PeriodicalId":13172,"journal":{"name":"HLA","volume":"106 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145774568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Characterisation of the Novel HLA-DQB1 Allele, HLA-DQB1*03:533 by Next Generation Sequencing","authors":"Sooho Yu, John Jeongseok Yang, Sohyeon Eom, Eun-Suk Kang","doi":"10.1111/tan.70510","DOIUrl":"10.1111/tan.70510","url":null,"abstract":"<div>\u0000 \u0000 <p><i>HLA-DQB1*03:533</i> differs from <i>HLA-DQB1*03:02:01:01</i> by a non-synonymous nucleotide substitution in exon 3.</p>\u0000 </div>","PeriodicalId":13172,"journal":{"name":"HLA","volume":"106 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145767906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Novel HLA-C*07:640:02 Allele, Identified by Sanger Dideoxy Nucleotide Sequencing in a Chinese Individual","authors":"Chuan-ming Lin, Meng-er Wang, Yan-ping Diao, Pei-pei Ding, Shui-ling Xie","doi":"10.1111/tan.70511","DOIUrl":"10.1111/tan.70511","url":null,"abstract":"<div>\u0000 \u0000 <p><i>HLA-C*07:640:02</i> differs from <i>HLA-C*07:640:01</i> by a single non-synonymous nucleotide change in exon 3.</p>\u0000 </div>","PeriodicalId":13172,"journal":{"name":"HLA","volume":"106 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145762601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Characterisation of a Novel HLA-A*02:558 Allele by Sequencing-Based Typing in a Chinese Individual","authors":"Jian-Ping Li, Xu Zhang, Feng-Qiu Lin, Xiao-Feng Li","doi":"10.1111/tan.70513","DOIUrl":"10.1111/tan.70513","url":null,"abstract":"<div>\u0000 \u0000 <p><i>HLA-A*02:558</i> differs from <i>HLA-A*02:06:01:01</i> by one nucleotide substitution in codon 96 in exon 3.</p>\u0000 </div>","PeriodicalId":13172,"journal":{"name":"HLA","volume":"106 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145762622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Identification of the Novel HLA-A*02:559 Allele by Sanger Dideoxy Nucleotide Sequencing","authors":"Xiao-Feng Li, Qiang Wang, Xu Zhang, Feng-Qiu Lin, Jian-Ping Li","doi":"10.1111/tan.70512","DOIUrl":"10.1111/tan.70512","url":null,"abstract":"<div>\u0000 \u0000 <p><i>HLA-A*02:559</i> differs from <i>HLA-A*02:01:01:01</i> by one nucleotide substitution in codon 188 in exon 4.</p>\u0000 </div>","PeriodicalId":13172,"journal":{"name":"HLA","volume":"106 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145762564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号