Pub Date : 2025-07-23DOI: 10.1109/TNB.2025.3591912
Saba Jalilian;Mohammad Javad Hoseinifar;Zahra Asadi;Elham Arkan;Nahid Moarrefzadeh;Faranak Aghaz
Prostate cancer (PCa) presents a significant challenge globally due to drug resistance and the severe side effects linked to conventional treatments. In this study, we developed vincristine-loaded nanoliposome-based lipids derived from Pseudomonas putida bacteria (VCR-NLPs) utilizing a thin-layer method. The produced bacteria-lipid-based nanoliposomes represented a critical advancement in drug delivery, offering superior drug encapsulation, controlled release, and enhanced biocompatibility. VCR-NLPs were thoroughly characterized, displaying a spherical morphology with an average particle size of approximately 145 nm, a final zeta potential of −13.1 mV, and a biphasic release profile of VCR. The formulation exhibited efficient drug loading, with 50% release at pH 7.4 and 70% at pH 6, reflecting pH-responsive release behavior tailored to the acidic tumor microenvironment, thereby enhancing therapeutic efficacy. Our flow-cytometric analysis confirmed an efficient induction of late-stage apoptosis in PC3 cells after treatment with VCR-NLPs. These findings suggest that Pseudomonas putida-Lipid-based VCR-NLPs offer a promising nanocarrier system for targeted prostate cancer therapy, due to inducing controlled release of VCR and improving biocompatibility of it, for clinical treatments.
{"title":"The Effectiveness of Lipids Derived From Pseudomonas Putida Bacteria in the Formulation of Nanoliposomes Enhances the Delivery of Vincristine for the Treatment of Prostate Cancer","authors":"Saba Jalilian;Mohammad Javad Hoseinifar;Zahra Asadi;Elham Arkan;Nahid Moarrefzadeh;Faranak Aghaz","doi":"10.1109/TNB.2025.3591912","DOIUrl":"10.1109/TNB.2025.3591912","url":null,"abstract":"Prostate cancer (PCa) presents a significant challenge globally due to drug resistance and the severe side effects linked to conventional treatments. In this study, we developed vincristine-loaded nanoliposome-based lipids derived from Pseudomonas putida bacteria (VCR-NLPs) utilizing a thin-layer method. The produced bacteria-lipid-based nanoliposomes represented a critical advancement in drug delivery, offering superior drug encapsulation, controlled release, and enhanced biocompatibility. VCR-NLPs were thoroughly characterized, displaying a spherical morphology with an average particle size of approximately 145 nm, a final zeta potential of −13.1 mV, and a biphasic release profile of VCR. The formulation exhibited efficient drug loading, with 50% release at pH 7.4 and 70% at pH 6, reflecting pH-responsive release behavior tailored to the acidic tumor microenvironment, thereby enhancing therapeutic efficacy. Our flow-cytometric analysis confirmed an efficient induction of late-stage apoptosis in PC3 cells after treatment with VCR-NLPs. These findings suggest that Pseudomonas putida-Lipid-based VCR-NLPs offer a promising nanocarrier system for targeted prostate cancer therapy, due to inducing controlled release of VCR and improving biocompatibility of it, for clinical treatments.","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"25 1","pages":"10-22"},"PeriodicalIF":4.4,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144698438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-26DOI: 10.1109/TNB.2025.3579900
{"title":"IEEE Transactions on NanoBioscience Information for Authors","authors":"","doi":"10.1109/TNB.2025.3579900","DOIUrl":"https://doi.org/10.1109/TNB.2025.3579900","url":null,"abstract":"","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"24 3","pages":"C3-C3"},"PeriodicalIF":3.7,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=11052636","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144492263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-16DOI: 10.1109/TNB.2025.3580172
Nazeer Abdul Azeez;Sung-Hoon Ahn
Drug delivery to the brain across the blood-brain barrier (BBB) has been a challenge for drugs unable to passively diffuse through it. Various parameters of the drugs contribute to the potency to cross the barrier made up of tight junctions of the epithelial cell membrane. For drugs with low permeability, novel nanoscale drug carriers have been developed to enhance delivery into the brain by circumventing the BBB. The carriers are fabricated in nanoscale for better penetration of the tight junctions in BBB. Understanding the physiology of the blood-brain barrier and the mechanism of molecular transport across it is crucial for designing effective drug carriers. Physiologically based pharmacokinetics (PBPK) modeling is a powerful tool for simulating the permeability of drugs and drug carriers across the BBB. The perfusion-limited kinetics and permeability-limited kinetics are two key equations that describe the transport of the drug into the brain and aiding in the determination of whether surface modifications to the drug carrier are necessary to improve the permeability. This review discusses the mechanisms of molecule transfer across the BBB, the parameters that filter drugs from the blood, the role of nanocarriers in enhancing permeability, the significance of PBPK modeling in extrapolating ${boldsymbol {i}n}~boldsymbol {vivo}$ permeability data of the drugs, and the recommended surface modifications to optimize drug delivery to the brain.
{"title":"Understanding the Crossing of Blood–Brain Barrier Using Nanocarriers: Current Trends and the Role of Physiologically Based Pharmacokinetic Modeling","authors":"Nazeer Abdul Azeez;Sung-Hoon Ahn","doi":"10.1109/TNB.2025.3580172","DOIUrl":"10.1109/TNB.2025.3580172","url":null,"abstract":"Drug delivery to the brain across the blood-brain barrier (BBB) has been a challenge for drugs unable to passively diffuse through it. Various parameters of the drugs contribute to the potency to cross the barrier made up of tight junctions of the epithelial cell membrane. For drugs with low permeability, novel nanoscale drug carriers have been developed to enhance delivery into the brain by circumventing the BBB. The carriers are fabricated in nanoscale for better penetration of the tight junctions in BBB. Understanding the physiology of the blood-brain barrier and the mechanism of molecular transport across it is crucial for designing effective drug carriers. Physiologically based pharmacokinetics (PBPK) modeling is a powerful tool for simulating the permeability of drugs and drug carriers across the BBB. The perfusion-limited kinetics and permeability-limited kinetics are two key equations that describe the transport of the drug into the brain and aiding in the determination of whether surface modifications to the drug carrier are necessary to improve the permeability. This review discusses the mechanisms of molecule transfer across the BBB, the parameters that filter drugs from the blood, the role of nanocarriers in enhancing permeability, the significance of PBPK modeling in extrapolating <inline-formula> <tex-math>${boldsymbol {i}n}~boldsymbol {vivo}$ </tex-math></inline-formula> permeability data of the drugs, and the recommended surface modifications to optimize drug delivery to the brain.","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"24 4","pages":"473-484"},"PeriodicalIF":4.4,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=11037453","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-05DOI: 10.1109/TNB.2025.3576227
Parvindar M. Sah;Smita G. Gite;Harshala S. Naik;Ratnamala Sonawane;Julia Nadrowska;Patrycja Golińska;Rajesh W. Raut;Aniket K. Gade
Silver doping into zinc oxide nanoparticles (Ag-ZnO NPs) were prepared via the co-precipitation method. The XRD analysis revealed the hexagonal structure characteristic of ZnO nanoparticles. The diminishing intensity of the peaks in Ag-ZnO NPs’ XRD pattern indicated the successful incorporation of Ag metal within the ZnO lattice. Elemental composition validation was performed through energy-dispersive X-ray spectroscopy (EDX), while FTIR spectroscopy elucidated the functional groups present in both ZnO and Ag nanoparticles. A judicious approach of 3% silver doping was employed to overcome silver’s toxicity potential at higher concentrations. Remarkably, the Ag-ZnO NPs exhibited exceptional, reusable photocatalytic prowess over four cycles in the degradation of methylene blue. Furthermore, the Ag-ZnO NPs showcased potent antibacterial efficacy against select pathogens, including Escherichia coli ATCC 27853, Salmonella typhi CT18, Staphylococcus aureus NCTC8325, and Bacillus subtilis QST 713. Notably, these nanoparticles also exhibited significant anticancer activity against Hep-G2, a human hepatoma cell line. Silver-doped zinc oxide emerges as a promising asset against wastewater dye pollution and holds promising applications in liver cancer.
{"title":"Ag-Doped ZnO Nanoparticles: A Versatile Multifunctional Nanomaterial for Anticancer, Antibacterial, and Recyclable Photocatalyst","authors":"Parvindar M. Sah;Smita G. Gite;Harshala S. Naik;Ratnamala Sonawane;Julia Nadrowska;Patrycja Golińska;Rajesh W. Raut;Aniket K. Gade","doi":"10.1109/TNB.2025.3576227","DOIUrl":"10.1109/TNB.2025.3576227","url":null,"abstract":"Silver doping into zinc oxide nanoparticles (Ag-ZnO NPs) were prepared via the co-precipitation method. The XRD analysis revealed the hexagonal structure characteristic of ZnO nanoparticles. The diminishing intensity of the peaks in Ag-ZnO NPs’ XRD pattern indicated the successful incorporation of Ag metal within the ZnO lattice. Elemental composition validation was performed through energy-dispersive X-ray spectroscopy (EDX), while FTIR spectroscopy elucidated the functional groups present in both ZnO and Ag nanoparticles. A judicious approach of 3% silver doping was employed to overcome silver’s toxicity potential at higher concentrations. Remarkably, the Ag-ZnO NPs exhibited exceptional, reusable photocatalytic prowess over four cycles in the degradation of methylene blue. Furthermore, the Ag-ZnO NPs showcased potent antibacterial efficacy against select pathogens, including Escherichia coli ATCC 27853, Salmonella typhi CT18, Staphylococcus aureus NCTC8325, and Bacillus subtilis QST 713. Notably, these nanoparticles also exhibited significant anticancer activity against Hep-G2, a human hepatoma cell line. Silver-doped zinc oxide emerges as a promising asset against wastewater dye pollution and holds promising applications in liver cancer.","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"24 4","pages":"465-472"},"PeriodicalIF":4.4,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144233997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-03DOI: 10.1109/TNB.2025.3576231
Uche A. K. Chude-Okonkwo;Athanasios V. Vasilakos
Myocardial infarction (MI) is one of the leading cardiovascular pathologies that often result in mortality. One of the methods to improve patient outcomes and lower mortality in MI occurrence is early detection. This requires access to individuals’ real-time vital cardiac signs to detect the onset of MI. However, most known vital cardiac signs and biomarkers of MI are either not always present in MI episodes or are not unique to MI. Hence, there is a need to develop a framework that can uniquely determine the onset of MI. This work proposes a framework for early detection of the MI onset that leverages the MI biomarker sensing capability of the Graphene-field effect transistor (G-FET), the remote vital cardiac indicators transmission ability of a communication network, and the real-time adaptive potential of recursive Bayesian updating based on an individual’s changing condition. The resultant posterior probability associated with the Bayesian updating, which is dynamically modified as new data is received in real-time, indicates the MI onset. This ensures early detection of MI. Considering an MI onset detection window of 30 to 60 minutes as a critical time to ensure that MI effects are salvageable, numerical results are provided. The numerical results demonstrate that the proposed framework provides early detection of MI onset, crucial to salvaging its effects and lowering mortality. The influence of some of the design parameters on the system performance is also evaluated.
{"title":"Real-Time Detection of Myocardial Infarction Onset Using Communication Network-Enabled Recursive Bayesian Updating","authors":"Uche A. K. Chude-Okonkwo;Athanasios V. Vasilakos","doi":"10.1109/TNB.2025.3576231","DOIUrl":"10.1109/TNB.2025.3576231","url":null,"abstract":"Myocardial infarction (MI) is one of the leading cardiovascular pathologies that often result in mortality. One of the methods to improve patient outcomes and lower mortality in MI occurrence is early detection. This requires access to individuals’ real-time vital cardiac signs to detect the onset of MI. However, most known vital cardiac signs and biomarkers of MI are either not always present in MI episodes or are not unique to MI. Hence, there is a need to develop a framework that can uniquely determine the onset of MI. This work proposes a framework for early detection of the MI onset that leverages the MI biomarker sensing capability of the Graphene-field effect transistor (G-FET), the remote vital cardiac indicators transmission ability of a communication network, and the real-time adaptive potential of recursive Bayesian updating based on an individual’s changing condition. The resultant posterior probability associated with the Bayesian updating, which is dynamically modified as new data is received in real-time, indicates the MI onset. This ensures early detection of MI. Considering an MI onset detection window of 30 to 60 minutes as a critical time to ensure that MI effects are salvageable, numerical results are provided. The numerical results demonstrate that the proposed framework provides early detection of MI onset, crucial to salvaging its effects and lowering mortality. The influence of some of the design parameters on the system performance is also evaluated.","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"24 4","pages":"485-497"},"PeriodicalIF":4.4,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Silver nanoparticles (AgNPs) are widely used for their antimicrobial properties but pose risks like environmental contamination and potential harm to human health. Nanoparticles’ small size facilitates translocation within the body, often bringing them into contact with blood. Most toxicological research focuses on chemically synthesized AgNPs (CAgNPs) and their effects on microbes and animal cells. Fewer studies explore biologically synthesized AgNPs (BAgNPs) on animal cells, and their impact on blood components is uncertain with varied findings due to differences in size and stability. This study examined BAgNPs’ effects on blood components in healthy and diseased states, using algae Parachlorella kessleri for synthesis. Nanoparticle size and morphology were assessed via TEM and UV-Vis spectrophotometry. Exposure to BAgNPs resulted in an increased number of echinocytes, reduced neutrophils, and decreased leukocyte viability. Unlike CAgNPs, BAgNPs did not increase macrophage proliferation. Differences in biological properties between BAgNPs and CAgNPs stem from their colloidal stability in varying environments. CAgNPs, stabilized electrostatically, exhibited greater aggregation in environments with higher salinity and lower pH, diminishing their biological effects in human blood. Hence, electrostatically stabilized chemically produced AgNPs may not be suitable for biomedical applications.
{"title":"Differences in Immunological Impact of Chemically and Biologically Synthesized Silver Nanoparticles","authors":"Vlasta Demeckova;Veronika Demcakova;Jana Sedlakova-Kadukova","doi":"10.1109/TNB.2025.3564822","DOIUrl":"10.1109/TNB.2025.3564822","url":null,"abstract":"Silver nanoparticles (AgNPs) are widely used for their antimicrobial properties but pose risks like environmental contamination and potential harm to human health. Nanoparticles’ small size facilitates translocation within the body, often bringing them into contact with blood. Most toxicological research focuses on chemically synthesized AgNPs (CAgNPs) and their effects on microbes and animal cells. Fewer studies explore biologically synthesized AgNPs (BAgNPs) on animal cells, and their impact on blood components is uncertain with varied findings due to differences in size and stability. This study examined BAgNPs’ effects on blood components in healthy and diseased states, using algae Parachlorella kessleri for synthesis. Nanoparticle size and morphology were assessed via TEM and UV-Vis spectrophotometry. Exposure to BAgNPs resulted in an increased number of echinocytes, reduced neutrophils, and decreased leukocyte viability. Unlike CAgNPs, BAgNPs did not increase macrophage proliferation. Differences in biological properties between BAgNPs and CAgNPs stem from their colloidal stability in varying environments. CAgNPs, stabilized electrostatically, exhibited greater aggregation in environments with higher salinity and lower pH, diminishing their biological effects in human blood. Hence, electrostatically stabilized chemically produced AgNPs may not be suitable for biomedical applications.","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"24 4","pages":"434-442"},"PeriodicalIF":4.4,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=10978870","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144011633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-24DOI: 10.1109/TNB.2025.3563382
Hoang Phuong Uyen Nguyen;Hoang Van Huy Dai;Anh Hue Luong;Wei-Chih Lin
This study highlights the structural, antioxidant, antibacterial, and anti-inflammatory properties of silver nanoparticles (AgNPs) and zinc oxide nanoparticles (ZnONPs), synthesized successfully using Hsiantsao aqueous extract as an eco-friendly alternative to traditional chemical methods. The antioxidant activity of the nanoparticles was assessed through DPPH, ABTS, and FRAP assays. The XRD spectra of biosynthesized silver nanoparticles (AgNPs) and zinc oxide nanoparticles (ZnONPs) are showed size average of 7 nm and 24-44 nm, respectively. AgNPs demonstrated notable antioxidant properties, achieving 70%±0.68 DPPH scavenging and 75%±0.82 ABTS inhibition at 0.1 mg/mL. ZnONPs showed superior efficacy, with 47.43%±0.68 DPPH scavenging and 80%±0.82 ABTS inhibition, as well as robust reducing power in the FRAP assay, comparable to standard ascorbic acid. Antibacterial assays revealed that AgNPs were particularly effective against Gram-positive bacteria, while ZnONPs exhibited activity against both Gram-positive and Gram-negative strains. Additionally, ZnONPs demonstrated exceptional anti-inflammatory potential, inhibiting protein denaturation by up to 91% at 0.01 mg/mL. These structural and functional characteristics position AgNPs and ZnONPs as promising candidates for biomedical applications. These findings underscore the versatility of AgNPs and ZnONPs in advancing modern healthcare solutions.
{"title":"Biosynthesis of Silver and Zinc Oxide Nanoparticles Using Platostoma palustre Aqueous Extract for Biomedical Applications","authors":"Hoang Phuong Uyen Nguyen;Hoang Van Huy Dai;Anh Hue Luong;Wei-Chih Lin","doi":"10.1109/TNB.2025.3563382","DOIUrl":"10.1109/TNB.2025.3563382","url":null,"abstract":"This study highlights the structural, antioxidant, antibacterial, and anti-inflammatory properties of silver nanoparticles (AgNPs) and zinc oxide nanoparticles (ZnONPs), synthesized successfully using Hsiantsao aqueous extract as an eco-friendly alternative to traditional chemical methods. The antioxidant activity of the nanoparticles was assessed through DPPH, ABTS, and FRAP assays. The XRD spectra of biosynthesized silver nanoparticles (AgNPs) and zinc oxide nanoparticles (ZnONPs) are showed size average of 7 nm and 24-44 nm, respectively. AgNPs demonstrated notable antioxidant properties, achieving 70%±0.68 DPPH scavenging and 75%±0.82 ABTS inhibition at 0.1 mg/mL. ZnONPs showed superior efficacy, with 47.43%±0.68 DPPH scavenging and 80%±0.82 ABTS inhibition, as well as robust reducing power in the FRAP assay, comparable to standard ascorbic acid. Antibacterial assays revealed that AgNPs were particularly effective against Gram-positive bacteria, while ZnONPs exhibited activity against both Gram-positive and Gram-negative strains. Additionally, ZnONPs demonstrated exceptional anti-inflammatory potential, inhibiting protein denaturation by up to 91% at 0.01 mg/mL. These structural and functional characteristics position AgNPs and ZnONPs as promising candidates for biomedical applications. These findings underscore the versatility of AgNPs and ZnONPs in advancing modern healthcare solutions.","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"24 4","pages":"421-433"},"PeriodicalIF":4.4,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The experimental and theoretical realization of 10% graphene doped ZnO/Graphene thin film alcohol sensor has been reported. The alcohol sensor has been fabricated by sol-gel method and theoretically verified by DFT-based first principle calculations. The quality of the fabricated device has been studied using SEM and UV measurements. To determine its figures-of-merit, the conductivity, transfer characteristics, and response measurements have been analyzed. In addition, the device has undergone three different exposures of alcohol concentrations such as Brandy, Whiskey, and Rum with varying exposure times.
{"title":"Fabrication and Characterization of ZnO/Graphene Thin Film Alcohol Sensor","authors":"Routu Santosh;Anuriddh Bahadur Yadav;Ball Mukund Mani Tripathi;Rahul Checker;Pankaj Kumar","doi":"10.1109/TNB.2025.3563456","DOIUrl":"10.1109/TNB.2025.3563456","url":null,"abstract":"The experimental and theoretical realization of 10% graphene doped ZnO/Graphene thin film alcohol sensor has been reported. The alcohol sensor has been fabricated by sol-gel method and theoretically verified by DFT-based first principle calculations. The quality of the fabricated device has been studied using SEM and UV measurements. To determine its figures-of-merit, the conductivity, transfer characteristics, and response measurements have been analyzed. In addition, the device has undergone three different exposures of alcohol concentrations such as Brandy, Whiskey, and Rum with varying exposure times.","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"24 4","pages":"404-410"},"PeriodicalIF":4.4,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143994810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-22DOI: 10.1109/TNB.2025.3563307
Hansa Gul;Haris Ahmed Khan;Zahida Nasreen;Nasir Assad;Syed Ali Turab;Muhammad Hanif
Iron oxide nanoparticles (Fe2O3 NPs) were successfully Synthesized in a green manner using Cucurbita pepo pulp extract as a natural capping and reducing agent. UV-vis spectroscopy verified the synthesis with a distinct absorption peak at 285 nm, while FTIR analysis revealed functional groups responsible for reduction and stabilization. X-ray diffraction (XRD) analysis confirmed the crystalline nature of the nanoparticles, with an average size of 21.5 nm. SEM and EDX analyses further validated the nanoparticles’ spherical morphology and elemental composition. Biosynthesized IONPs exhibited notable antibacterial activity against multidrug-resistant bacterial strains such as Klebsiella pneumoniae and Pseudomonas aeruginosa. The inhibition zones ranged between 5–22 mm for Klebsiella pneumoniae and from 4 to 12 mm for Pseudomonas aeruginosa, depending on the concentration of the nanoparticles. Hematological evaluations demonstrated strong anticoagulant and thrombolytic properties. Iron oxide nanoparticles effectively inhibited blood coagulation at $40~mu $ g/mL and showed significant thrombolytic activity by dissolving preformed clots at $50~mu $ g/mL. The biosynthesized IONPs showed remarkable antioxidant activity that was comparable to standard. This study underscores the potential of Cucurbita pepo as a sustainable and eco-friendly resource for synthesizing multifunctional IONPs. The results suggest promising applications to address antibiotic resistance and manage blood-related disorders. Furthermore, the findings highlight the critical role of green nanotechnology in the advancement of environmentally sustainable and biocompatible nanomaterials for diverse biomedical applications.
{"title":"Exploring the Antibacterial, Anticoagulant, and Hemolytic Potential of Green-Synthesized Fe2O3 Nanoparticles by Cucurbita pepo Pulp","authors":"Hansa Gul;Haris Ahmed Khan;Zahida Nasreen;Nasir Assad;Syed Ali Turab;Muhammad Hanif","doi":"10.1109/TNB.2025.3563307","DOIUrl":"10.1109/TNB.2025.3563307","url":null,"abstract":"Iron oxide nanoparticles (Fe2O3 NPs) were successfully Synthesized in a green manner using Cucurbita pepo pulp extract as a natural capping and reducing agent. UV-vis spectroscopy verified the synthesis with a distinct absorption peak at 285 nm, while FTIR analysis revealed functional groups responsible for reduction and stabilization. X-ray diffraction (XRD) analysis confirmed the crystalline nature of the nanoparticles, with an average size of 21.5 nm. SEM and EDX analyses further validated the nanoparticles’ spherical morphology and elemental composition. Biosynthesized IONPs exhibited notable antibacterial activity against multidrug-resistant bacterial strains such as Klebsiella pneumoniae and Pseudomonas aeruginosa. The inhibition zones ranged between 5–22 mm for Klebsiella pneumoniae and from 4 to 12 mm for Pseudomonas aeruginosa, depending on the concentration of the nanoparticles. Hematological evaluations demonstrated strong anticoagulant and thrombolytic properties. Iron oxide nanoparticles effectively inhibited blood coagulation at <inline-formula> <tex-math>$40~mu $ </tex-math></inline-formula>g/mL and showed significant thrombolytic activity by dissolving preformed clots at <inline-formula> <tex-math>$50~mu $ </tex-math></inline-formula>g/mL. The biosynthesized IONPs showed remarkable antioxidant activity that was comparable to standard. This study underscores the potential of Cucurbita pepo as a sustainable and eco-friendly resource for synthesizing multifunctional IONPs. The results suggest promising applications to address antibiotic resistance and manage blood-related disorders. Furthermore, the findings highlight the critical role of green nanotechnology in the advancement of environmentally sustainable and biocompatible nanomaterials for diverse biomedical applications.","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"24 4","pages":"411-420"},"PeriodicalIF":4.4,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144011637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：