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Obesity: An Impact with Cardiovascular and Cerebrovascular Diseases 肥胖:对心脑血管疾病的影响
Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-10-13 DOI: 10.1007/s12291-023-01157-w
Savi Dutta, A. K. Singhal, Varsha Suryan, Nimai Chand Chandra
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引用次数: 0
Role of Grape (Vitis vinifera) Extracts of Different Cultivars Against γ-Radiation Induced DNA Damage and Gene Expression in Human Lymphocytes 不同品种葡萄提取物对γ-辐射诱导的人淋巴细胞DNA损伤及基因表达的作用
Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-10-03 DOI: 10.1007/s12291-023-01154-z
Indrani Singha, Dipak Kumar Poria, Partho Sarothi Ray, Subir Kumar Das
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引用次数: 0
Expression of miR-145 and miR-18b in Peripheral Blood Samples of Head and Neck Cancer Patients. 头颈部癌症患者外周血样本中miR-145和miR-18b的表达。
IF 1.5 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-10-01 Epub Date: 2023-02-07 DOI: 10.1007/s12291-023-01119-2
Arun Kumar Yadav, Nishant Singh, Sanjeev Kumar Yadav, M L B Bhatt, Amit Pandey, Dev Kumar Yadav, Sanjay Yadav

Head and neck squamous cell carcinomas (HNSCC) is one of the most prevalent type of cancer known in Indian population. Studies are needed to identify the early biomarkers for HNSCC. MicroRNAs (miRNAs) are non-coding RNA molecules, expression of which can be used as biomarker for early diagnosis of HNSCC. For miRNA profiling total RNA, which also contained small RNAs were isolated from ten HNSCC tissue samples and adjacent control. Purity and concentration of eluted RNA was assessed using the NanoDrop1000® spectrophotometer, Reverse Transcription reaction was carried out with megaplex RT primers of pool A and pool B and the expression of selected miRNAs (miR-143/145 and miR-18a/b) was measured using TaqMan primers specific for mature miRNAs. Our study showed dramatic downregulation in expression of two miRNAs, miR-18b and miR-145 in blood samples of HNSCC patients, which are inhibitor of tumorigenesis and can be targeted as biomarker of HNSCC pathogenesis therefore developing avenues for miRNA role in prognosis and therapeutics.

Supplementary information: The online version contains supplementary material available at 10.1007/s12291-023-01119-2.

头颈部鳞状细胞癌(HNSCC)是已知在印度人群中最常见的癌症类型之一。需要进行研究以确定HNSCC的早期生物标志物。微小RNA(miRNA)是非编码RNA分子,其表达可作为HNSCC早期诊断的生物标志物。对于miRNA图谱,从10个HNSCC组织样本和相邻对照中分离出总RNA,其中也含有小RNA。使用NanoDrop1000®分光光度计评估洗脱RNA的纯度和浓度,使用池A和池B的megaplex RT引物进行逆转录反应,并使用成熟miRNA特异性的TaqMan引物测量选定miRNA(miR-143/145和miR-18a/B)的表达。我们的研究显示,HNSCC患者血液样本中两种miRNA,miR-18b和miR-145的表达显著下调,这两种miRNAs是肿瘤发生的抑制剂,可以作为HNSCC发病机制的生物标志物,因此为miRNA在预后和治疗中的作用开辟了途径。补充信息:在线版本包含补充材料,请访问10.1007/s12291-023-0119-2。
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引用次数: 0
Association of the Human Leptin Receptor Gene (rs1137101; Gln223Arg) Polymorphism and Circulating Leptin in Patients with Metabolic Syndrome in the Indian Population. 人类瘦素受体基因(rs1137101;Gln223Arg)多态性与印度人群代谢综合征患者循环瘦素的相关性。
IF 1.5 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-10-01 Epub Date: 2022-08-09 DOI: 10.1007/s12291-022-01065-5
Deepak Parchwani, Sagar Dholariya, Digishaben D Patel, Ashishkumar Agravatt, Jayant Uperia, Tanishk Parchwani, Ragini Singh, Madhuri Radadiya, Yash Desai

Phenotypic expression of metabolic syndrome is precipitated by environmental variables along with the individual genetic susceptibility to the obesogenic environment and growing body of evidence suggest a paramount role of adipocytokines. Therefore, identifying the genetic influence on circulation leptin levels and clarifying genotype-phenotype correlation of rs1137101 {Leptin receptor gene (LEPR) Gln223Arg (Q223R; A668G)} in metabolic syndrome were the primary objective of this study. A total of 447 adult participants, including 214 metabolic syndrome patients and 233 healthy controls, were genotyped using polymerase chain reaction-restriction fragment length polymorphism method to unravel the effects of genetic risk loci {Leptin receptor gene; Gln223Arg (Q223R; A668G); rs1137101} on the occurrence of metabolic syndrome in consort with circulation leptin levels. Suitable descriptive statistics was used for different variables. The genotype frequencies were found to be in Hardy-Weinberg equilibrium for both cases (p > 0.2722) as well as in controls (p > 0.2331). However, genotype (x2: 11.26, 2 d.f. p = 0.0036) and allele distribution (x2: 10.51, 2 d.f. p: 0.0012) of the LEPR Gln223Arg (Q223R; A668G) differed significantly between cases and controls. Gln/Arg genotype (OR = 1.6099; 95% CI = 1.0847-2.3893; p value = 0.0181), Arg/Arg genotype (OR = 2.8121; 95% CI = 1.4103-5.6074; p value = 0.0033) and R allele (OR = 1.5875; 95% CI = 1.1996-2.1008; p value = 0.0012) were significantly associated with increased risk of metabolic syndrome in univariate analysis. Further a multivariate logistic regression adjusted for potential confounders showed that Arg/Arg genotype (OR = 1.9; 95% CI = 1.271-2.639; p-value < 0.05) and Gln/Arg (OR: 1.3; 95% CI = 0.873-2.034; p value < 0.05) have a significant risk for the occurrence of the metabolic syndrome. A progressive increase in the serum leptin levels from major homozygous alleles to minor homozygous alleles were observed indicating that rs1137101 modify the serum leptin concentrations in patients with metabolic syndrome. These findings provide enough evidence of a significant association of LEPR Gln223Arg (Q223R; A668G) polymorphism in the LepR gene in Indian patients with increased risk of metabolic syndrome for R allele and Arg/Arg homozygote. Thus, rs1137101 might be a pleiotropic locus for metabolic syndrome and its components in studied population.

代谢综合征的表型表达是由环境变量以及个体对肥胖环境的遗传易感性促成的,越来越多的证据表明脂肪细胞因子发挥着重要作用。因此,本研究的主要目的是确定遗传对循环瘦素水平的影响,并阐明rs1137101{瘦素受体基因(LEPR)Gln223Arg(Q223R;A668G)}在代谢综合征中的基因型-表型相关性。共有447名成年参与者,包括214名代谢综合征患者和233名健康对照,使用聚合酶链式反应限制性片段长度多态性方法进行基因分型,以揭示遗传风险基因座{瘦素受体基因;Gln223Arg(Q223R;A668G);rs1137101}对循环瘦素水平的配偶代谢综合征发生的影响。对不同的变量使用了适当的描述性统计。两种情况的基因型频率均处于Hardy-Weinberg平衡(p > 0.2722)以及对照组(p > 0.2331)。然而,基因型(x2:11.26,2 d.f.p = 0.0036)和LEPR Gln223Arg(Q223R;A668G)的等位基因分布(x2:10.51,2 d.f.p:0.0012)在病例和对照组之间存在显著差异。Gln/Arg基因型(OR = 1.6099;95%CI = 1.0847-2.3893;p值 = 0.0181)、Arg/Arg基因型(OR = 2.8121;95%CI = 1.4103-5.6074;p值 = 0.0033)和R等位基因(OR = 1.5875;95%CI = 1.1996-21008;p值 = 0.0012)与代谢综合征风险增加显著相关。此外,对潜在混杂因素进行调整的多变量逻辑回归显示,Arg/Arg基因型(OR = 1.9;95%CI = 1.271-2.639;p值
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引用次数: 0
Mid-Regional Proadrenomedullin Levels in Primary Immunodeficiencies Complicated with Pulmonary Manifestations. 原发性免疫缺陷合并肺部表现的肾上腺髓质素中期水平。
IF 1.5 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-10-01 Epub Date: 2022-09-09 DOI: 10.1007/s12291-022-01061-9
Elif Azarsiz, Neslihan Karaca, Necil Kutukculer

The development of lower respiratory complications in children with primary immunodeficiencies characterized by recurrent infections significantly contributes to morbidity and mortality. This is clinically more important and specific in the evaluation of prognosis. The inflammatory response that develops throughout the clinical process can cause the release of several biomarkers. This study aimed to evaluate the inflammatory biomarker "mid-regional pro-adrenomedullin (MR-proADM)" levels by distribution of lower respiratory tract complications. Plasma MR-proADM levels were measured in children with (n = 52) and without (n = 103) lower respiratory tract complications. The complicated group was also evaluated as "infective and non-infective" groups. The median MR-proADM levels were higher in the complicated cases (p = 0.175). It was 205.5 (73.4- 562.6) ng/L in the infective group while it was 96.1 (26.1-43.3) ng/L in the non-infective group and the difference between the two groups was statistically significant (p = 0.003). The predictive value of MR-proADM (AUC = 0.749, p = 0.003) was statistically significant compared to CRP (AUC = 0.330, p = 0.040) and SAA (AUC = 0.261, p = 0.004) in the infective group. This study evidences that the MR-proADM levels are higher in PID cases with infective pulmonary complications. Among other markers, MR-proADM appears to be a particularly good predictive inflammation marker for these children.

Supplementary information: The online version contains supplementary material available at 10.1007/s12291-022-01061-9.

以反复感染为特征的原发性免疫缺陷儿童的下呼吸道并发症的发展显著增加了发病率和死亡率。这在临床上对预后的评估更为重要和具体。在整个临床过程中产生的炎症反应可以导致几种生物标志物的释放。本研究旨在通过下呼吸道并发症的分布来评估炎症生物标志物“中部肾上腺髓质素原(MR-proADM)”水平。在患有(n = 52)和不带(n = 103)下呼吸道并发症。复杂组也被评估为“感染和非感染”组。复杂病例的MR-proADM水平中位数较高(p = 感染组为205.5(73.4-562.6)ng/L,非感染组为96.1(26.1-43.3)ng/L = 0.003)。MR-proADM的预测值(AUC = 0.749,p = 0.003)与CRP(AUC)相比具有统计学意义 = 0.330,p = 0.040)和SAA(AUC = 0.261,p = 0.004)。本研究表明,在伴有感染性肺部并发症的PID患者中,MR-proADM水平较高。在其他标志物中,MR-proADM似乎是这些儿童特别好的炎症预测标志物。补充信息:在线版本包含补充材料,可访问10.1007/s12291-022-01061-9。
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引用次数: 0
Association Between Blood Lead Levels and Thyroid Function: An Updated Systematic Review and Meta-Analysis. 血铅水平与甲状腺功能的关系:最新系统综述和荟萃分析。
IF 1.5 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-10-01 Epub Date: 2023-01-26 DOI: 10.1007/s12291-023-01113-8
Rakesh Balachandar, Ankit Viramgami, Bhavani Shankara Bagepally, Kuldip Upadhyay

Chronic Lead (Pb) exposure is associated with disrupting certain endocrine levels and is referred to as an endocrine disruptor. Thyroid hormones, involved in regulating numerous physiological functions, are reported with conflicting associations with chronic Pb exposure. This study broadly evaluated the association between long-term lead exposure and thyroid function parameters. In this systematic review, the observational studies documenting the changes in thyroid function parameters between the chronically Pb-exposed and controls were systematically identified from PubMed-Medline, Scopus, and Embase digital databases from the beginning to March 31, 2022. The protocol was previously registered with PROSPERO (ID: CRD42022315520) and executed following PRISMA 2020 guidelines. The random-effects model was used to analyze the mean differences in thyroid function parameters between groups. The classical I2 statistic was applied to assess heterogeneity, while the Newcastle Ottawa Scale was used to assess the risk of various biases. Where data allowed, sub-group, sensitivity, and meta-regression analyses were carried out. The current systematic review included fifteen observational studies. The Pb-exposed have a higher mean blood Pb level [i.e. 28.07 (95% CI 21.43-34.72) µg/dl], significantly higher free T3 [(i.e. 0.48 pg/dl (95% CI 0.05-0.95)] and trend of higher T3. T4, FT4, and TSH levels than controls with high heterogeneity (I2 > 85%). Considering the important role of thyroid hormone in multiple biological functions, the present findings emphasize the requisite for high-quality studies to investigate the association between levels of thyroid function parameters among individuals known for cumulative exposure to Pb.

Supplementary information: The online version contains supplementary material available at 10.1007/s12291-023-01113-8.

长期接触铅与干扰某些内分泌水平有关,被称为内分泌干扰物。据报道,参与调节多种生理功能的甲状腺激素与慢性铅暴露有着相互矛盾的关系。这项研究广泛评估了长期铅暴露与甲状腺功能参数之间的关系。在这项系统综述中,从2022年初到3月31日,从PubMed Medline、Scopus和Embase数字数据库中系统地确定了记录慢性铅暴露者和对照者甲状腺功能参数变化的观察性研究。该方案之前已在PROSPERO(ID:CRD42022315520)注册,并按照PRISMA 2020指南执行。随机效应模型用于分析各组甲状腺功能参数的平均差异。经典的I2统计用于评估异质性,而纽卡斯尔-渥太华量表用于评估各种偏差的风险。在数据允许的情况下,进行分组、敏感性和元回归分析。目前的系统综述包括15项观察性研究。铅暴露者的平均血液铅水平较高[即28.07(95%CI 21.43-34.72)µg/dl],游离T3显著较高[即0.48 pg/dl(95%CI 0.05-0.95)],T3呈升高趋势。T4、FT4和TSH水平高于具有高度异质性的对照组(I2 > 85%)。考虑到甲状腺激素在多种生物学功能中的重要作用,目前的研究结果强调了高质量研究的必要性,以调查已知累积接触铅的个体甲状腺功能参数水平之间的关系。补充信息:在线版本包含补充材料,可访问10.1007/s12291-023-0113-8。
{"title":"Association Between Blood Lead Levels and Thyroid Function: An Updated Systematic Review and Meta-Analysis.","authors":"Rakesh Balachandar, Ankit Viramgami, Bhavani Shankara Bagepally, Kuldip Upadhyay","doi":"10.1007/s12291-023-01113-8","DOIUrl":"10.1007/s12291-023-01113-8","url":null,"abstract":"<p><p>Chronic Lead (Pb) exposure is associated with disrupting certain endocrine levels and is referred to as an endocrine disruptor. Thyroid hormones, involved in regulating numerous physiological functions, are reported with conflicting associations with chronic Pb exposure. This study broadly evaluated the association between long-term lead exposure and thyroid function parameters. In this systematic review, the observational studies documenting the changes in thyroid function parameters between the chronically Pb-exposed and controls were systematically identified from PubMed-Medline, Scopus, and Embase digital databases from the beginning to March 31, 2022. The protocol was previously registered with PROSPERO (ID: CRD42022315520) and executed following PRISMA 2020 guidelines. The random-effects model was used to analyze the mean differences in thyroid function parameters between groups. The classical <i>I</i><sup>2</sup> statistic was applied to assess heterogeneity, while the Newcastle Ottawa Scale was used to assess the risk of various biases. Where data allowed, sub-group, sensitivity, and meta-regression analyses were carried out. The current systematic review included fifteen observational studies. The Pb-exposed have a higher mean blood Pb level [i.e. 28.07 (95% CI 21.43-34.72) µg/dl], significantly higher free T<sub>3</sub> [(i.e. 0.48 pg/dl (95% CI 0.05-0.95)] and trend of higher T<sub>3</sub>. T<sub>4</sub>, FT<sub>4</sub>, and TSH levels than controls with high heterogeneity (<i>I</i><sup>2</sup> > 85%). Considering the important role of thyroid hormone in multiple biological functions, the present findings emphasize the requisite for high-quality studies to investigate the association between levels of thyroid function parameters among individuals known for cumulative exposure to Pb.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12291-023-01113-8.</p>","PeriodicalId":13280,"journal":{"name":"Indian Journal of Clinical Biochemistry","volume":"38 4","pages":"426-436"},"PeriodicalIF":1.5,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10516803/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41116706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of Expression Level of miR-3135b-5p in Blood Samples of Breast Cancer Patients Experiencing Chemotherapy-Induced Cardiotoxicity. miR-3135b-5p在经历化疗诱导的心脏毒性的癌症患者血液样本中表达水平的评估。
IF 1.5 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-10-01 Epub Date: 2022-09-06 DOI: 10.1007/s12291-022-01075-3
Nasrin Zare, Nasim Dana, Azam Mosayebi, Golnaz Vaseghi, Shaghayegh Haghjooy Javanmard

The efficacy of chemotherapeutics in the treatment of breast cancer is limited by cardiotoxicity, which could lead to irreversible heart failure. The evaluation of miRNA levels as a vital biomarker could predict cardiotoxicity induced by chemotherapy. According to our previous meta-analysis study on patients with heart failure, we found that miR-3135b had a significant increase in patients with heart failure. Therefore, the present study aimed to evaluate the expression level of miR-3135b in the blood sample of patients experiencing chemotherapy-induced cardiotoxicity. Blood samples were collected from breast cancer patients or breast cancer patients who had received chemotherapy and had not experienced any chemotherapy-induced cardiotoxicity (N = 37, control group) and breast cancer patients experiencing chemotherapy-induced cardiotoxicity after chemotherapy (N = 33). The expression level of miR-3135b was evaluated using real-time polymerase chain reaction (RT-PCR). The 2-ΔCt values of miR-3135b were compared between two groups. We observed a significant increase in the expression level of miR-3135b between patients experiencing chemotherapy-induced cardiotoxicity and the control group (P = 0.0001). Besides, the ejection fraction parameter was correlated with the expression level of miR-3135b (r = 0.5 and P = 0.0001). To sum up, miR-3135b might be useful as a promising circulating biomarker in predicting cardiotoxicity induced by chemotherapy. However, more studies are needed to validate miR-3135b as a biomarker for the diagnosis of chemotherapy-induced cardiotoxicity.

Supplementary information: The online version contains supplementary material available at 10.1007/s12291-022-01075-3.

化疗药物治疗癌症的疗效受到心脏毒性的限制,心脏毒性可能导致不可逆转的心力衰竭。miRNA水平作为一种重要的生物标志物的评估可以预测化疗诱导的心脏毒性。根据我们之前对心力衰竭患者的荟萃分析研究,我们发现miR-3135b在心力衰竭患者中显著增加。因此,本研究旨在评估化疗诱导的心脏毒性患者血液样本中miR-3135b的表达水平。从接受过化疗且未经历任何化疗引起的心脏毒性的乳腺癌症患者或癌症患者采集血液样本(N = 37,对照组)和化疗后出现化疗诱导的心脏毒性的癌症患者(N = 33)。使用实时聚合酶链式反应(RT-PCR)评估miR-3135b的表达水平。比较两组之间miR-3135b的2-ΔCt值。我们观察到,在化疗诱导的心脏毒性患者和对照组之间,miR-3135b的表达水平显著增加(P = 0.0001)。此外,射血分数参数与miR-3135b的表达水平相关(r = 0.5和P = 0.0001)。总之,miR-3135b可能是预测化疗诱导的心脏毒性的一种有前途的循环生物标志物。然而,还需要更多的研究来验证miR-3135b作为诊断化疗诱导的心脏毒性的生物标志物。补充信息:在线版本包含补充材料,请访问10.1007/s12291-022-1075-3。
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引用次数: 0
Reference Range of Plasma Fatty Acids in North Indian Pregnant Population. 北印度孕妇血浆脂肪酸的参考范围。
IF 1.5 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-10-01 Epub Date: 2022-08-17 DOI: 10.1007/s12291-022-01071-7
Shubham Thakur, Amrit Pal Kaur, Kanwardeep Singh, Rajpinder Kaur, Manpreet Kaur, Subheet Kumar Jain

Reference values for Fatty Acids (FAs) are not well defined in the Indian population. Therefore, it is critical to establish FAs reference range for the healthy non-pregnant and pregnant Indian population. The present multi-centric, and cross-sectional study determines the 95% reference interval for FAs in an apparently pregnant Indian population and compare it to the healthy non-pregnant women. Physicians identified 164 reference individuals as healthy (56 non-pregnant and 108 pregnant) at various government and private hospitals of northern India. The 95th and 97.5th percentile reference limits were used to estimate the 95 percentile of the reference distribution. The reference ranges observed for Alpha-linolenic acid (0.29-0.42%; 0.36-0.58%), Docosahexaenoic-acid (3.38-4.23%; 3.8-4.55%), Eicosapentaenoic-acid (1.24-1.76%; 1.09-1.62%), Docosapentaenoic-acid-3 (0.61-0.69%; 0.65-0.76%), Linoleic-acid (18.44-20.75%; 19.51-21.88%), gamma-linolenic-acid (0.24-0.35%; 0.32-0.42%), Eicosatrienoic-acid (0.26-0.32%; 0.34-0.39%), Arachidonic-acid (9.29-11.02%; 10.02-11.56%), Docosatetraenoic-acid (0.62-0.89%; 0.79-1.09%), Docosapentaenoic-acid-6 (0.23-0.31%; 0.33-0.41%), Eicosatrienoic-acid (1.17-1.41%; 1.43-1.74%), Eicosenoic-acid (0.28-0.38%; 0.37-0.49%), Nervonic-acid (1.39-1.69%; 1.41-1.74%), Palmitoleic-acid (1.17-1.58%; 2-2.66%), Oleic-acid (19.8-22.26%; 19.68-22.94%), Myristic-acid (1.16-1.68%; 0.82-1.3%), Palmitic-acid (20.05-21.8%; 20.7-22.43%), Stearic-acid (11.34-12.56%; 10.29-11.02%), Arachidic-acid (0.17-0.2%; 0.18-0.23%), Lignoceric-acid (0.81-1.08%; 0.77-1.08%), trans-palmitoleic-acid (0.22-0.29%; 0.26-0.37%), trans-oleic-acid (0.55-0.72%; 0.68-0.84%), trans-linoleic-acid (0.38-0.54%; 0.42-0.59%) respectively for non-pregnant and pregnant women. Furthermore, total FAs were significantly (p ≤ 0:05) higher in women aged 31-45 years than in women aged 16-30 years. whereas, there was no significant change in total FAs profile based on omega-supplementation, diet category, preterm-birth history, and gestation period. Thus, the current study provides information about an individual who is deficient in FAs and the dose required to increase FA concentrations in the body.

脂肪酸(FA)的参考值在印度人群中没有很好的定义。因此,为健康的非孕妇和孕妇印度人群建立FAs参考范围至关重要。目前的多中心、横断面研究确定了明显怀孕的印度人群中FA的95%参考区间,并将其与健康的非孕妇进行了比较。医生在印度北部的多家政府和私立医院确定了164名健康参考者(56名未怀孕,108名怀孕)。第95和97.5百分位参考限值用于估计参考分布的95百分位。观察到的α-亚麻酸(0.29-0.42%;0.36-0.58%)、二十二碳六烯酸(3.38-4.23%;3.8-4.55%)、二十碳五烯酸(1.24-1.76%;1.09-1.62%)、二十一碳五烯酸-3(0.61-0.69%;0.65-0.76%)、亚油酸(18.44-20.75%;19.51-21.88%)、γ-亚麻酸,花生四烯酸(9.29-11.02%;10.02-11.56%)、二十二碳四烯酸(0.62-0.89%;0.79-1.09%)、二十二C五烯酸-6(0.23-0.31%;0.33-0.41%)、二十碳三烯酸(1.17-1.41%;1.43-1.74%)、二十烯酸(0.28-0.38%;0.37-0.49%)、神经酸(1.39-1.69%;1.41-1.74%),棕榈油酸(1.17-1.58%;2-2.66%)、油酸(19.8-22.26%;19.68-22.94%)、肉豆蔻酸(1.16-1.68%;0.82-1.3%),非孕妇和孕妇分别为棕榈酸(20.05-21.8%;20.7-22.43%)、硬脂酸(11.34-12.56%;10.29-11.02%)、花生四烯酸(0.17-0.2%;0.18-0.23%)、木质纤维素酸(0.81-1.08%;0.77-1.08%)、反式棕榈油酸(0.22-0.29%;0.26-0.37%)、反性油酸(0.55-0.72%;0.68-0.84%)和反式亚油酸(0.38-0.54%;0.42-0.59%)。此外,总FA显著(p ≤ 0:05)高于16-30岁女性。然而,基于ω补充剂、饮食类别、早产史和妊娠期的总脂肪酸状况没有显著变化。因此,目前的研究提供了有关FA缺乏者的信息,以及增加体内FA浓度所需的剂量。
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引用次数: 0
Efficacy of serum anti-mullerian hormone (AMH) levels for prediction of polycystic ovary syndrome (PCOS) and its association with clinical, biochemical and hormonal parameters. 血清抗苗勒管激素(AMH)水平预测多囊卵巢综合征(PCOS)的疗效及其与临床、生化和激素参数的关系。
IF 1.5 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-10-01 Epub Date: 2022-09-06 DOI: 10.1007/s12291-022-01058-4
Sajad Sumji, Abid Bhat, Aafia Rashid, Rohina Bashir, Imtiyaz A Wani, Vishnu Vasudevan, Tajali Sehar, Mohd Ashraf Ganie

Anti-mullerian hormone (AMH) has been proposed to add significance to diagnosis of PCOS in case of ambiguity. However, variable cutoffs of AHM among PCOS women have been reported. Using case-control design, this study investigated the diagnostic threshold of serum AMH levels among age matched 113 PCOS and 75 normo-ovulatory women and its correlation with clinical, hormonal and ultrasonographic parameters.PCOS was defined as per Rotterdam criteria 2003. Results depicted the mean serum AMH level to be significantly higher in PCOS group (7.84 ± 3.67vs. 3.23 ± 1.56 ng/mL) than controls. The AMH levels were positively(p = 0.001) associated with ovarian volume (r = 0.521) as well as number of ovarian follicles(r = 0.461). Further, serum AMH levels showed a positive correlation with luteinizing hormone/follicle stimulating hormone (LH/FSH) ratio (r = 0.206, p = 0.02), but no correlation significant with age, BMI,FG score and testosterone levels. As per receiver operating characteristic (ROC) curve, cut-off was worked out to be 3.76 ng/ml with 86.7% sensitivity and 62.7% specificity. The mean level of AMH were highest among PCOS women with phenotype A (12.67 ± 3.46 ng/ml) with least among PCOS women displaying phenotype B(7.28 ± 1.60 ng/ml) where there is absence of PCOM. In conclusion, serum AMH levels are highly predictive of PCOM and high LH/FSH ratio among PCOS women and may be a potent diagnostic marker of ovarian dysfunction either alone or in conjunction with other tools.

Supplementary information: The online version contains supplementary material available at 10.1007/s12291-022-01058-4.

抗苗勒管激素(AMH)已被提出在不明确的情况下对PCOS的诊断增加意义。然而,据报道,多囊卵巢综合征妇女AHM的临界值各不相同。本研究采用病例对照设计,研究了113例PCOS和75例正常排卵妇女血清AMH水平的诊断阈值及其与临床、激素和超声参数的相关性。PCOS是根据2003年鹿特丹标准定义的。结果显示PCOS组的平均血清AMH水平显著升高(7.84 ± 3.67与3.23 ± 1.56ng/mL)。AMH水平呈阳性(p = 0.001)与卵巢体积(r = 0.521)以及卵泡数(r = 血清AMH水平与黄体生成素/卵泡刺激素(LH/FSH)比值呈正相关(r = 0.206,p = 0.02),但与年龄、BMI、FG评分和睾酮水平无显著相关性。根据受试者工作特性(ROC)曲线,截止值为3.76ng/ml,灵敏度为86.7%,特异性为62.7%。A型多囊卵巢综合征妇女的AMH平均水平最高(12.67 ± 3.46ng/ml),而在显示表型B的PCOS妇女中最少(7.28 ± 1.60ng/ml),其中不存在PCOM。总之,血清AMH水平对PCOS妇女的PCOM和高LH/FSH比率具有高度预测性,并且可能是卵巢功能障碍的有效诊断标志,无论是单独使用还是与其他工具联合使用。补充信息:在线版本包含补充材料,请访问10.1007/s12291-022-1058-4。
{"title":"Efficacy of serum anti-mullerian hormone (AMH) levels for prediction of polycystic ovary syndrome (PCOS) and its association with clinical, biochemical and hormonal parameters.","authors":"Sajad Sumji, Abid Bhat, Aafia Rashid, Rohina Bashir, Imtiyaz A Wani, Vishnu Vasudevan, Tajali Sehar, Mohd Ashraf Ganie","doi":"10.1007/s12291-022-01058-4","DOIUrl":"10.1007/s12291-022-01058-4","url":null,"abstract":"<p><p>Anti-mullerian hormone (AMH) has been proposed to add significance to diagnosis of PCOS in case of ambiguity. However, variable cutoffs of AHM among PCOS women have been reported. Using case-control design, this study investigated the diagnostic threshold of serum AMH levels among age matched 113 PCOS and 75 normo-ovulatory women and its correlation with clinical, hormonal and ultrasonographic parameters.PCOS was defined as per Rotterdam criteria 2003. Results depicted the mean serum AMH level to be significantly higher in PCOS group (7.84 ± 3.67vs. 3.23 ± 1.56 ng/mL) than controls. The AMH levels were positively(p = 0.001) associated with ovarian volume (r = 0.521) as well as number of ovarian follicles(r = 0.461). Further, serum AMH levels showed a positive correlation with luteinizing hormone/follicle stimulating hormone (LH/FSH) ratio (r = 0.206, p = 0.02), but no correlation significant with age, BMI,FG score and testosterone levels. As per receiver operating characteristic (ROC) curve, cut-off was worked out to be 3.76 ng/ml with 86.7% sensitivity and 62.7% specificity. The mean level of AMH were highest among PCOS women with phenotype A (12.67 ± 3.46 ng/ml) with least among PCOS women displaying phenotype B(7.28 ± 1.60 ng/ml) where there is absence of PCOM. In conclusion, serum AMH levels are highly predictive of PCOM and high LH/FSH ratio among PCOS women and may be a potent diagnostic marker of ovarian dysfunction either alone or in conjunction with other tools.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12291-022-01058-4.</p>","PeriodicalId":13280,"journal":{"name":"Indian Journal of Clinical Biochemistry","volume":"38 4","pages":"457-465"},"PeriodicalIF":1.5,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10516813/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41116801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Acton-Prolongatum Stimulated Blood Steroid Profile in Apparently Healthy Asian Indian Women of Reproductive-Age Group. 长效阿克顿刺激明显健康的亚洲-印度育龄妇女的血液类固醇图谱。
IF 1.5 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-10-01 Epub Date: 2022-04-30 DOI: 10.1007/s12291-022-01039-7
Vijaya Sarathi, Anudeep Reddy, Sunanda Tirupati, Kauser Jumkhawala

Blood steroid profile is a recently introduced test in India that is commercially available through a few diagnostic laboratories. In adult women, ACTH-stimulated steroid panel helps to differentiate polycystic ovary syndrome (PCOS) from nonclassical forms of CAH. However, the interpretation of ACTH-stimulated steroid panels is often impeded by the limited availability of reference ranges. Here, we report the adrenal steroid levels after stimulation with Acton Prolongatum in Asian Indian women of reproductive age. This prospective study was conducted at a tertiary health care center in the Southern part of India. Apparently healthy women in the reproductive age group with regular menstrual cycles (21-35 days) at least over the last 6 months were included. All participants received intramuscular Acton Prolongatum® (Pfizer) in the morning hours during the follicular phase and the steroid profile was analyzed by liquid chromatography-tandem mass spectrometry in a blood sample collected 60-min later. The study included 32 apparently healthy women. The mean age of the study population was 22.19 ± 4.36 years. None of the participants experienced any adverse events during the procedure. The median (range) serum cortisol, 17α-hydroxyprogesterone, 11-deoxycortisol, and corticosterone were 22.65 (14.3-37.21) μg/dl, 99.72 (47.21-344.71) ng/dl, 287.2 (74.41-530.61) ng/dl and 728.04 (118.74-1708.2) respectively. In conclusion, this is the first report of the response of adrenal steroids measured by LC-MS/MS at 60 min after Acton Prolongatum in Asian Indian women of the reproductive age group. However, further larger studies are warranted to establish more robust ACTH-stimulated reference ranges for steroid profile in Indian women.

血液类固醇图谱是最近在印度引入的一种检测方法,可通过一些诊断实验室进行商业化。在成年女性中,促肾上腺皮质激素刺激的类固醇有助于区分多囊卵巢综合征(PCOS)和非经典形式的CAH。然而,ACTH刺激的类固醇组的解释往往受到参考范围有限的阻碍。在这里,我们报告了亚洲-印度育龄妇女使用长效阿克顿刺激后的肾上腺类固醇水平。这项前瞻性研究是在印度南部的一家三级医疗保健中心进行的。生殖年龄组中至少在过去6个月内月经周期正常(21-35天)的明显健康女性也包括在内。所有参与者在卵泡期的早晨接受肌肉注射Acton Extentiatum®(辉瑞),并通过液相色谱-串联质谱法分析60分钟后采集的血样中的类固醇成分。这项研究包括32名明显健康的女性。研究人群的平均年龄为22.19岁 ± 4.36年。没有一名参与者在手术过程中出现任何不良事件。血清皮质醇、17α-羟基孕甾酮、11脱氧皮质醇和皮质酮的中位数(范围)分别为22.65(14.3-37.21)μg/dl、99.72(47.21-344.71)ng/dl、287.2(74.41-530.61)ng/del和728.04(118.74-1708.2)。总之,这是首次报道生殖年龄组的亚洲-印度妇女在Acton Longitum后60分钟通过LC-MS/MS测量肾上腺类固醇的反应。然而,有必要进一步进行更大规模的研究,以确定印度女性中ACTH刺激的类固醇水平的参考范围。
{"title":"Acton-Prolongatum Stimulated Blood Steroid Profile in Apparently Healthy Asian Indian Women of Reproductive-Age Group.","authors":"Vijaya Sarathi, Anudeep Reddy, Sunanda Tirupati, Kauser Jumkhawala","doi":"10.1007/s12291-022-01039-7","DOIUrl":"10.1007/s12291-022-01039-7","url":null,"abstract":"<p><p>Blood steroid profile is a recently introduced test in India that is commercially available through a few diagnostic laboratories. In adult women, ACTH-stimulated steroid panel helps to differentiate polycystic ovary syndrome (PCOS) from nonclassical forms of CAH. However, the interpretation of ACTH-stimulated steroid panels is often impeded by the limited availability of reference ranges. Here, we report the adrenal steroid levels after stimulation with Acton Prolongatum in Asian Indian women of reproductive age. This prospective study was conducted at a tertiary health care center in the Southern part of India. Apparently healthy women in the reproductive age group with regular menstrual cycles (21-35 days) at least over the last 6 months were included. All participants received intramuscular Acton Prolongatum® (Pfizer) in the morning hours during the follicular phase and the steroid profile was analyzed by liquid chromatography-tandem mass spectrometry in a blood sample collected 60-min later. The study included 32 apparently healthy women. The mean age of the study population was 22.19 ± 4.36 years. None of the participants experienced any adverse events during the procedure. The median (range) serum cortisol, 17α-hydroxyprogesterone, 11-deoxycortisol, and corticosterone were 22.65 (14.3-37.21) μg/dl, 99.72 (47.21-344.71) ng/dl, 287.2 (74.41-530.61) ng/dl and 728.04 (118.74-1708.2) respectively. In conclusion, this is the first report of the response of adrenal steroids measured by LC-MS/MS at 60 min after Acton Prolongatum in Asian Indian women of the reproductive age group. However, further larger studies are warranted to establish more robust ACTH-stimulated reference ranges for steroid profile in Indian women.</p>","PeriodicalId":13280,"journal":{"name":"Indian Journal of Clinical Biochemistry","volume":"38 4","pages":"541-544"},"PeriodicalIF":1.5,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10516798/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41126383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Indian Journal of Clinical Biochemistry
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