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Study of MicroRNA (miR-221-3p, miR-133a-3p, and miR-9-5p) Expressions in Oral Submucous Fibrosis and Squamous Cell Carcinoma. 口腔黏膜下纤维化和鳞状细胞癌中的微RNA(miR-221-3p、miR-133a-3p 和 miR-9-5p)表达研究
IF 2.1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-01-01 Epub Date: 2022-04-29 DOI: 10.1007/s12291-022-01035-x
Shweta Ukey, Ankit Jain, Shailendra Dwivedi, Chinmayee Choudhury, Jeewan Ram Vishnoi, Ankita Chugh, Purvi Purohit, Puneet Pareek, Poonam Elhence, Sanjeev Misra, Praveen Sharma

Oral squamous cell carcinoma (OSCC) is one of the common types of cancer. Its progression follows a transition from oral potentially malignant disorders (OPMDs) such as oral submucous fibrosis (OSMF). Epigenetic modifiers, especially microRNAs (miRNAs), have an appreciable role in the regulation of various carcinogenic pathways which are being used as biomarkers. miRNAs may also be helpful in the differentiation of oral submucous fibrosis from oral squamous cell carcinoma. Three miRNAs, miR-221-3p, miR133a-3p, and miR-9-5p, were found differentially expressed in many cancers in the literature search supported by our preliminary database search-based screening. The literature and our functional enrichment analysis in an earlier study have reported these miRNAs to regulate carcinogenesis at various steps. In the present study, the expression of these miRNAs was examined in 34 histopathologically confirmed OSCC, 30 OSMF, and 29 control (healthy volunteers) human samples. There was a significant downregulation of miRNA-133a-3p in OSCC compared to OSMF and controls, whereas there was up-regulation in oral submucous fibrosis compared to controls. There was no significant difference in the expression of miR-221-3p between OSCC and OSMF, but an upregulation in OSCC compared to controls. miR-9-5p was also found upregulated in both OSCC and OSMF. Further, miR-133a-3p expression was negatively correlated with age, smoking, drinking status, and AJCC staging, whereas miR-9-5p expression was only positively associated with tobacco/ areca nut chewing. The ROC plots, logistic regression model generated, and the correlation between the expression of miR-9-5p and miR-133a-3p in blood and tissue suggests that these could be used as risk stratification biomarkers.

口腔鳞状细胞癌(OSCC)是常见的癌症类型之一。它的发病过程是从口腔潜在恶性疾病(OPMD)(如口腔黏膜下纤维化(OSMF))过渡而来。表观遗传修饰因子,尤其是微小核糖核酸(miRNA),在各种致癌途径的调控中发挥着重要作用,被用作生物标志物。在文献检索中发现,miR-221-3p、miR133a-3p 和 miR-9-5p 这三种 miRNA 在许多癌症中都有不同程度的表达。文献和我们在早期研究中进行的功能富集分析表明,这些 miRNA 可在不同阶段调控癌变。本研究检测了 34 例经组织病理学证实的 OSCC、30 例 OSMF 和 29 例对照组(健康志愿者)人体样本中这些 miRNA 的表达。与 OSMF 和对照组相比,miRNA-133a-3p 在 OSCC 中明显下调,而与对照组相比,miRNA-133a-3p 在口腔黏膜下纤维化中上调。在 OSCC 和 OSMF 中,miR-221-3p 的表达没有明显差异,但与对照组相比,OSCC 中的 miR-221-3p 表达上调。此外,miR-133a-3p 的表达与年龄、吸烟、饮酒状况和 AJCC 分期呈负相关,而 miR-9-5p 的表达仅与咀嚼烟草/山苍子呈正相关。ROC图、逻辑回归模型的生成以及血液和组织中miR-9-5p和miR-133a-3p表达量之间的相关性表明,这些指标可用作风险分层生物标志物。
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引用次数: 3
Glutamine-Driven Metabolic Adaptation to COVID-19 Infection. 谷氨酰胺驱动的COVID-19感染代谢适应
IF 2.1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-01-01 DOI: 10.1007/s12291-022-01037-9
Hüseyin Aydın, Yusuf Kenan Tekin, İlhan Korkmaz, Gülaçan Tekin, Sefa Yurtbay, Sami Keleş, Nezih Hekim

Background: COVID-19 is known to be transmitted by direct contact, droplets or feces/orally. There are many factors which determines the clinical progression of the disease. Aminoacid disturbance in viral disease is shown in many studies. İn this study we aimed to evaluate the change of aminoacid metabolism especially the aspartate, glutamine and glycine levels which have been associated with an immune defence effect in viral disease.

Methods: Blood samples from 35 volunteer patients with COVID-19, concretized diagnosis was made by oropharyngeal from nazofaringeal swab specimens and reverse transcriptase-polymerase chain reaction, and 35 control group were analyzed. The amino acid levels were measured with liquid chromatography-mass spectrometry technology. Two groups were compared by Kolmogorov-Smirnov analysis, Kruskal-Wallis and the Mann-Whitney U. The square test was used to evaluate the tests obtained by counting, and the error level was taken as 0.05.

Results: The average age of the patient and control group were 48.5 ± 14.9 and 48.8 ± 14.6 years respectively. The decrease in aspartate (p = 5.5 × 10-9) and glutamine levels (p = 9.0 × 10-17) were significiantly in COVID group, whereas Glycine (p = 0.243) increase was not significiant.

Conclusions: Metabolic pathways, are affected in rapidly dividing cells in viral diseases which are important for immun defence. We determined that aspartate, glutamine and glycine levels in Covid 19 patients were affected by the warburg effect, malate aspartate shuttle, glutaminolysis and pentose phosphate pathway. Enteral or parenteral administration of these plasma amino acid levels will correct the duration and pathophysiology of the patients' stay in hospital and intensive care.

背景:已知COVID-19通过直接接触、飞沫或粪便/口服传播。有许多因素决定疾病的临床进展。许多研究表明病毒性疾病中存在氨基酸紊乱。İn本研究旨在评估在病毒性疾病中与免疫防御作用相关的氨基酸代谢,特别是天冬氨酸、谷氨酰胺和甘氨酸水平的变化。方法:对35例自愿感染COVID-19的患者进行血液采集,经口咽咽咽拭子标本和逆转录-聚合酶链反应进行具体诊断,并对35例对照组进行分析。采用液相色谱-质谱联用技术测定氨基酸水平。两组比较采用Kolmogorov-Smirnov分析、Kruskal-Wallis分析和Mann-Whitney U.检验,计数所得检验采用平方检验,误差水平取0.05。结果:患者和对照组的平均年龄分别为48.5±14.9岁和48.8±14.6岁。COVID组天冬氨酸水平(p = 5.5 × 10-9)和谷氨酰胺水平(p = 9.0 × 10-17)显著降低,甘氨酸水平(p = 0.243)升高不显著。结论:在病毒性疾病中,代谢途径在细胞快速分裂过程中受到影响,在免疫防御中起重要作用。我们发现,新冠肺炎患者体内天冬氨酸、谷氨酰胺和甘氨酸水平受warburg效应、苹果酸-天冬氨酸穿梭、谷氨酰胺水解和戊糖磷酸途径的影响。这些血浆氨基酸水平的肠内或肠外管理将纠正患者住院和重症监护的时间和病理生理。
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引用次数: 3
Identification of Plasma Proteomic Biomarkers in Patients with Mild Cognitive Impairment. 鉴定轻度认知障碍患者的血浆蛋白质组生物标志物
IF 2.1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-01-01 Epub Date: 2022-02-15 DOI: 10.1007/s12291-022-01023-1
Vineeta Singh, Vijaya Nath Mishra, Mahendra Kumar Thakur

Plasma proteomic profiling may provide novel biomarkers for the identification of mild cognitive impairment (MCI). The early diagnosis of MCI still remains a challenging task due to its diverse origin. Currently, molecular approaches have been used to identify MCI diversified origin as its onset is governed by a variety of molecular changes. Therefore, we aimed to find out molecular alteration in plasma using proteomics in patients with MCI for early detection of prodromal Alzheimer's disease (AD). To achieve this, we performed two-dimensional (2-D) gel electrophoresis coupled with MALDI-TOF/MS, which is used to analyze the differentially expressed proteins. In our study, we found three significantly altered proteins. Out of three differentially expressed proteins, one was downregulated and two were upregulated in MCI individuals as compared to control. Further, In silico analysis showed that identified proteins are involved in pathways such as complement and coagulation cascades, platelet activation and AD. STRING interaction network analysis revealed that the majority of proteins including apolipoprotein E (APO-E) have a common association with Transthyretin (TTR) and fibrinogen chain beta (FGB) protein. This suggests that APO-E, TTR and FGB are the key proteins with which other proteins interact to exert other biological functions. Conclusively, these proteins showing differential expression in the plasma might be used as a potent signature in blood for the diagnosis of MCI individuals.

血浆蛋白质组分析可为轻度认知障碍(MCI)的鉴定提供新的生物标志物。由于MCI的发病原因多种多样,因此早期诊断MCI仍然是一项具有挑战性的任务。目前,由于 MCI 的发病受多种分子变化的影响,分子方法已被用于识别 MCI 的不同起源。因此,我们的目的是利用蛋白质组学发现 MCI 患者血浆中的分子变化,以便早期发现阿尔茨海默病(AD)的前兆。为此,我们进行了二维(2-D)凝胶电泳并结合 MALDI-TOF/MS,用于分析差异表达的蛋白质。在我们的研究中,我们发现了三种明显改变的蛋白质。在三种差异表达的蛋白质中,与对照组相比,MCI 患者的一种蛋白质下调,两种蛋白质上调。此外,硅学分析表明,确定的蛋白质参与了补体和凝血级联、血小板活化和 AD 等通路。STRING相互作用网络分析显示,包括载脂蛋白E(APO-E)在内的大多数蛋白质与转甲状腺素(TTR)和纤维蛋白原链β(FGB)蛋白有共同的关联。这表明,APO-E、TTR 和 FGB 是与其他蛋白质相互作用以发挥其他生物功能的关键蛋白质。最后,这些在血浆中呈现差异表达的蛋白质可作为诊断 MCI 患者的有效血液特征。
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引用次数: 0
Identification of h-TERT Promoter Mutations in Germline DNA from North Indian Lung Carcinoma Patients. 北印度肺癌患者种系 DNA 中 h-TERT 启动子突变的鉴定
IF 2.1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-01-01 Epub Date: 2022-05-19 DOI: 10.1007/s12291-022-01047-7
Rajendra Prasad, Sonia Panchal, Isha Rani, Jai Kishan, Gaurav Parashar

Lung cancer is a severe and the leading cause of cancer related deaths worldwide. The recurrent h-TERT promoter mutations have been implicated in various cancer types. Thus, the present study is extended to analyze h-TERT promoter mutations from the North Indian lung carcinoma patients. Total 20 histopathologically and clinically confirmed cases of lung cancer were enrolled in this study. The genomic DNA was extracted from venous blood and subjected to amplification using appropriate h-TERT promoter primers. Amplified PCR products were subjected for DNA Sanger sequencing for the identification of novel h-TERT mutations. Further, these identified h-TERT promoter mutations were analysed for the prediction of pathophysiological consequences using bioinformatics tools such as Tfsitescan and CIIDER. The average age of patients was 45 ± 8 years which was categorized in early onset of lung cancer with predominance of male patients by 5.6 fold. Interestingly, h-TERT promoter mutations were observed highly frequent in lung cancer. Identified mutations include c. G272A, c. T122A, c. C150A, c. 123 del C, c. C123T, c. G105A, c. 107 Ins A, c. 276 del C corresponding to -168 G>A, -18 T>A, -46 C>A, -19 del C, -19 C>T, -1 G>A, -3 Ins A, -172 del C respectively from the translation start site in the promoter of the telomerase reverse transcriptase gene which are the first time reported in germline genome from lung cancer. Strikingly, c. -18 T>A [C.T122A] was found the most prevalent variant with 75% frequency. Notwithstanding, other mutations viz c. -G168A [c. G272A] and c. -1 G>A [c. G105A] were found to be at 35% and 15% frequency respectively whilst the rest of the mutations were present at 10% and 5% frequency. Additionally, bioinformatics analysis revealed that these mutations can lead to either loss or gain of various transcription factor binding sites in the h-TERT promoter region. Henceforth, these mutations may play a pivotal role in h-TERT gene expression. Taken together, these identified novel promoter mutations may alter the epigenetics and subsequently various transcription factor binding sites which are of great functional significance. Thereby, it is plausible that these germline mutations may involve either as predisposing factor or direct participation in the pathophysiology of lung cancer through entangled molecular mechanisms.

肺癌是一种严重的癌症,也是全球癌症相关死亡的主要原因。反复出现的 h-TERT 启动子突变与多种癌症类型都有关联。因此,本研究扩展分析了北印度肺癌患者的 h-TERT 启动子突变。共有 20 例经组织病理学和临床确诊的肺癌患者参与了本研究。从静脉血中提取基因组 DNA,并使用适当的 h-TERT 启动子引物进行扩增。扩增的 PCR 产物经 DNA Sanger 测序,以鉴定新型 h-TERT 基因突变。此外,还使用 Tfsitescan 和 CIIDER 等生物信息学工具对这些已确定的 h-TERT 启动子突变进行分析,以预测其病理生理后果。患者的平均年龄为 45 ± 8 岁,属于早发性肺癌,男性患者占多数,比例为 5.6 倍。有趣的是,h-TERT 启动子突变在肺癌中非常常见。已发现的突变包括 c. G272A、c. T122A、c. C150A、c. 123 del C、c. C123T、c. G105A、c. 107 Ins A、c.276 del C,分别对应于端粒酶反转录酶基因启动子翻译起始位点的-168 G>A、-18 T>A、-46 C>A、-19 del C、-19 C>T、-1 G>A、-3 Ins A、-172 del C,这在肺癌种系基因组中是首次报道。引人注目的是,c. -18 T>A [C.T122A]是最常见的变异,频率为75%。G168A [c. G272A]和 c. -1 G>A [c. G105A]的频率分别为35%和15%,而其他变异的频率分别为10%和5%。此外,生物信息学分析表明,这些突变可导致 h-TERT 启动子区域中各种转录因子结合位点的缺失或增益。因此,这些突变可能在h-TERT基因表达中起着关键作用。综上所述,这些已发现的新型启动子突变可能会改变表观遗传学,进而改变具有重要功能意义的各种转录因子结合位点。因此,这些种系突变可能是肺癌的易感因素,也可能通过纠缠不清的分子机制直接参与肺癌的病理生理学。
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引用次数: 0
Association of Angiotensin II Type 1 Receptor (AT1R) Gene Polymorphism with Angiotensin II Serum Levels in Patients with Essential Hypertension. 本质性高血压患者血管紧张素 II 1 型受体 (AT1R) 基因多态性与血管紧张素 II 血清水平的关系。
IF 2.1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-01-01 Epub Date: 2022-04-30 DOI: 10.1007/s12291-022-01041-z
M Prasad, D Rajarajeswari, K Ramlingam, R Viswakumar, B Suneel, Jyothi Conjeevaram, P Aruna, Nusrath Fathima, Sandeep Kumar Vishwakarma, Aleem Ahmed Khan

Essential hypertension (EH) is a multifactorial, polygenic condition, and is one of the most important comorbidities that contributes to stroke, myocardial infarction, cardiac failure, and renal failure. The continuous increasing rate of morbidity and mortality associated with EH presents an unmet need of population-based studies to explore pathophysiology as well as newer strategies for better diagnosis, prognosis and treatment. This study aimed to determine genotype and allele frequencies of A1166C polymorphism of AT1R gene in Indian patients with EH and correlated with serum levels of Angiotensin II. A total of 200 patients with EH and 200 age- and gender-matched control individuals were included in this study from the General Medicine Department Outpatient at Narayana Medical College and Hospital, Nellore, Andhra Pradesh, India. Patients with systolic blood pressure (SBP) ≥ 140 mmHg and/or diastolic blood pressure (DBP) ≥ 90 mmHg were considered as hypertensive. The findings of this study revealed significantly increased risk of C/A heterozygote and allele C in both men and women. Moreover, both men and women patients with EH showed higher serum levels of Angiotensin II with C/A as well as AA genotypes. These findings indicate a significant association of 1166 C/A polymorphism of the AT1R gene with increased risk of hypertension in Indian population.

继发性高血压(EH)是一种多因素、多基因疾病,是导致中风、心肌梗塞、心力衰竭和肾功能衰竭的最重要合并症之一。与 EH 相关的发病率和死亡率持续上升,这就需要开展以人群为基础的研究,以探索病理生理学以及更新的诊断、预后和治疗策略。本研究旨在确定印度 EH 患者 AT1R 基因 A1166C 多态性的基因型和等位基因频率,以及与血清血管紧张素 II 水平的相关性。本研究共纳入了 200 名 EH 患者和 200 名年龄与性别匹配的对照组患者,这些患者均来自印度安得拉邦内洛尔市纳拉亚那医学院和医院的全科门诊部。收缩压 (SBP) ≥ 140 mmHg 和/或舒张压 (DBP) ≥ 90 mmHg 的患者被视为高血压患者。研究结果显示,C/A 杂合子和等位基因 C 在男性和女性中的风险都明显增加。此外,男性和女性 EH 患者血清中血管紧张素 II 水平较高的基因型均为 C/A 和 AA。这些研究结果表明,在印度人群中,AT1R 基因的 1166 C/A 多态性与高血压风险的增加有重要关联。
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引用次数: 0
Mosaic Recombination Inflicted Various SARS-CoV-2 Lineages to Emerge into Novel Virus Variants: a Review Update. Mosaic重组导致各种严重急性呼吸系统综合征冠状病毒2型谱系出现新的病毒变体:综述更新。
IF 2.1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2022-12-17 DOI: 10.1007/s12291-022-01109-w
Pushpendra Singh, Kuldeep Sharma, Dipika Shaw, Anudita Bhargava, Sanjay Singh Negi

Human Coronaviruses (hCoVs) belongs to the enormous and dissimilar family of positive-sense, non-segmented, single-stranded RNA viruses. The RNA viruses are prone to high rates of mutational recombination resulting in emergence of evolutionary variant to alter various features including transmissibility and severity. The evolutionary changes affect the immune escape and reduce effectiveness of diagnostic and therapeutic measures by becoming undetectable by the currently available diagnostics and refractory to therapeutics and vaccines. Whole genome sequencing studies from various countries have adequately reported mosaic recombination between different lineage strain of SARS-CoV-2 whereby RNA dependent RNA polymerase (RdRp) gene reconnects with a homologous RNA strand at diverse position. This all lead to evolutionary emergence of new variant/ lineage as evident with the emergence of XBB in India at the time of writing this review. The continuous periodical genomic surveillance is utmost required for understanding the various lineages involved in recombination to emerge into hybrid variant. This may further help in assessing virus transmission dynamics, virulence and severity factor to help health authorities take appropriate timely action for prevention and control of any future COVID-19 outbreak.

人类冠状病毒(hCoV)属于一个巨大而不同的阳性、非分段、单链RNA病毒家族。RNA病毒容易发生高比率的突变重组,导致进化变体的出现,从而改变各种特征,包括传播性和严重性。进化的变化会影响免疫逃逸,并降低诊断和治疗措施的有效性,因为目前可用的诊断方法无法检测到这些变化,并且对治疗方法和疫苗不敏感。来自不同国家的全基因组测序研究充分报道了严重急性呼吸系统综合征冠状病毒2型不同谱系株之间的镶嵌重组,其中RNA依赖性RNA聚合酶(RdRp)基因在不同位置与同源RNA链重新连接。这一切都导致了新变种/谱系的进化出现,在撰写本综述时,印度出现了XBB。持续的定期基因组监测对于理解参与重组以形成杂交变体的各种谱系是至关重要的。这可能进一步有助于评估病毒传播动态、毒力和严重性因素,以帮助卫生当局及时采取适当行动,预防和控制任何未来的新冠肺炎疫情。
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引用次数: 0
Proceedings of ACBICON 2022 ACBICON 2022会议录
IF 2.1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2022-12-01 DOI: 10.1007/s12291-022-01106-z
{"title":"Proceedings of ACBICON 2022","authors":"","doi":"10.1007/s12291-022-01106-z","DOIUrl":"https://doi.org/10.1007/s12291-022-01106-z","url":null,"abstract":"","PeriodicalId":13280,"journal":{"name":"Indian Journal of Clinical Biochemistry","volume":"97 1","pages":"1 - 152"},"PeriodicalIF":2.1,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80529826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Cross Sectional Study of Vitamin D Levels in Western Rajasthan and Meta-Analysis for Estimation of Vitamin D LevelsIn the PDF, in Header of all pages, Journal title should be abbreviated as "Ind J Clin Biochem" 西拉贾斯坦邦维生素D水平的横断面研究和维生素D水平估计的荟萃分析在PDF中,在所有页面的标题中,期刊标题应缩写为“Ind J临床生物化学”。
IF 2.1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2022-10-06 DOI: 10.1007/s12291-022-01074-4
Surjit Singh, Divesh Jalan, P. Bhardwaj, Praveen Sharma, A. Elhence
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引用次数: 0
SARS-CoV-2 IgG Antibody and its Clinical Correlates in Convalescent Plasma Donors: An Indian Experience. 恢复期血浆捐献者的 SARS-CoV-2 IgG 抗体及其临床相关性:印度的经验
IF 1.5 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2022-10-01 Epub Date: 2021-11-18 DOI: 10.1007/s12291-021-01012-w
Pvsn Kiran Kumar, Mithu Banerjee, Archana Bajpayee, Saptarishi Mandal, Prasenjit Mitra, Praveen Sharma, Sanjeev Misra, Pankaj Bhardwaj

SARS-CoV-2, a novel coronavirus, emerged a year ago in Wuhan, China causing a new pandemic. Convalescent plasma therapy has been applied previously to many infectious diseases and has shown a successful result. This study was planned to assess the Anti-SARS-CoV-2 IgG antibody levels in convalescent COVID-19 patients. In this study, serum samples from 210 persons infected by SARS-CoV-2, treated and discharged from the hospital were collected. Anti-SARS-CoV-2 IgG antibody levels were detected using a chemiluminescence assay. A directory of convalescent plasma donors was created. Anti-SARS-CoV-2 IgG antibody levels vary substantially in the study population with a mean of 51.2 AU/ml. On comparing the serum anti-SARS-CoV-2 IgG antibody levels, a significant difference was observed between the subjects who had cough and those who did not (p = 0.0004). Similar significant findings were found with total protein and globulin levels on comparing the individuals with different antibody status (positive, negative and equivocal). The middle-aged and old age people had high Ab titres compared to younger individuals and the duration of the hospital stay was found to be positively correlated with the anti-SARS-CoV-2 IgG antibody. Cough, age and duration of the hospital stay was found to play a significant role in the development of Anti-SARS-CoV-2 IgG levels. Further, the data suggests that blood groups have a lesser impact on the severity of disease and the development of antibodies. Patients who present with the cough are more likely to develop antibodies.

SARS-CoV-2 是一种新型冠状病毒,一年前在中国武汉出现,引发了一场新的大流行。康复血浆疗法曾被应用于多种传染病的治疗,并取得了良好的效果。本研究计划评估 COVID-19 康复期患者的抗 SARS-CoV-2 IgG 抗体水平。本研究收集了 210 名感染 SARS-CoV-2 并接受治疗后出院的患者的血清样本。采用化学发光法检测抗 SARS-CoV-2 IgG 抗体水平。建立了康复血浆捐献者名录。研究人群的抗 SARS-CoV-2 IgG 抗体水平差异很大,平均值为 51.2 AU/ml。在比较血清抗 SARS-CoV-2 IgG 抗体水平时,发现有咳嗽的受试者与没有咳嗽的受试者之间存在显著差异(p = 0.0004)。在比较不同抗体状态(阳性、阴性和不确定)的人的总蛋白和球蛋白水平时,也发现了类似的重大发现。与年轻人相比,中老年人的抗体滴度较高,而且住院时间与抗 SARS-CoV-2 IgG 抗体呈正相关。研究发现,咳嗽、年龄和住院时间在抗 SARS-CoV-2 IgG 水平的发展中起着重要作用。此外,数据还表明,血型对疾病严重程度和抗体产生的影响较小。出现咳嗽的患者更容易产生抗体。
{"title":"SARS-CoV-2 IgG Antibody and its Clinical Correlates in Convalescent Plasma Donors: An Indian Experience.","authors":"Pvsn Kiran Kumar, Mithu Banerjee, Archana Bajpayee, Saptarishi Mandal, Prasenjit Mitra, Praveen Sharma, Sanjeev Misra, Pankaj Bhardwaj","doi":"10.1007/s12291-021-01012-w","DOIUrl":"10.1007/s12291-021-01012-w","url":null,"abstract":"<p><p>SARS-CoV-2, a novel coronavirus, emerged a year ago in Wuhan, China causing a new pandemic. Convalescent plasma therapy has been applied previously to many infectious diseases and has shown a successful result. This study was planned to assess the Anti-SARS-CoV-2 IgG antibody levels in convalescent COVID-19 patients. In this study, serum samples from 210 persons infected by SARS-CoV-2, treated and discharged from the hospital were collected. Anti-SARS-CoV-2 IgG antibody levels were detected using a chemiluminescence assay. A directory of convalescent plasma donors was created. Anti-SARS-CoV-2 IgG antibody levels vary substantially in the study population with a mean of 51.2 AU/ml. On comparing the serum anti-SARS-CoV-2 IgG antibody levels, a significant difference was observed between the subjects who had cough and those who did not (p = 0.0004). Similar significant findings were found with total protein and globulin levels on comparing the individuals with different antibody status (positive, negative and equivocal). The middle-aged and old age people had high Ab titres compared to younger individuals and the duration of the hospital stay was found to be positively correlated with the anti-SARS-CoV-2 IgG antibody. Cough, age and duration of the hospital stay was found to play a significant role in the development of Anti-SARS-CoV-2 IgG levels. Further, the data suggests that blood groups have a lesser impact on the severity of disease and the development of antibodies. Patients who present with the cough are more likely to develop antibodies.</p>","PeriodicalId":13280,"journal":{"name":"Indian Journal of Clinical Biochemistry","volume":"37 4","pages":"423-431"},"PeriodicalIF":1.5,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599420/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39761882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Breathomics - the Way Forward Towards a Mask Free World. 呼吸组学——通往无面具世界的道路。
IF 2.1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2022-10-01 Epub Date: 2022-10-05 DOI: 10.1007/s12291-022-01092-2
Mithu Banerjee, Jyoti Shekhawat, Praveen Sharma
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引用次数: 1
期刊
Indian Journal of Clinical Biochemistry
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