Indian Journal of Medical Microbiology最新文献

英文中文

Evaluation of BD Phoenix and VITEK 2 for direct and routine antimicrobial susceptibility from positive blood culture bottles BD Phoenix和VITEK-2对阳性血培养瓶直接和常规抗菌药物敏感性的评价。

IF 1.4 4区医学 Q4 IMMUNOLOGY

Indian Journal of Medical Microbiology

Pub Date : 2025-07-01 DOI: 10.1016/j.ijmmb.2025.100906

Naima Sirad , Mustafa Altay Atalay , Pınar Sağıroğlu

Objectives

This study aims to comparatively evaluate the reliability of BD Phoenix and VITEK 2 systems for direct, routine and standard antimicrobial susceptibility testing (AST) of ESKAPE isolates from positive blood culture bottles, with a primary focus on the interpretation of results into susceptible, intermediate, or resistant categories.

Methods

A total of 128 ESKAPE isolates from positive blood culture bottles were subjected to direct, routine and standard AST. Direct AST (DAST) and routine AST (RAST) were performed using BD Phoenix and VITEK 2 automated systems. DAST was conducted using a bacterial pellet obtained directly from positive blood cultures, while RAST used a colony after subculture. Results of both were compared based on categorical agreement (CA) and discrepancies. RAST results were further evaluated against standard AST (SAST), performed via the Kirby-Bauer disc diffusion assay using 24 h-grown colonies.

Findings and results

The AST categorical agreements (CA) of DAST with the RAST using BD Phoenix automated system for Enterobacterales, Non-fermenting, and Gram-positive cocci (S. aureus and Enterococci spp.) were 95.3 %, 100 %, and 100 % respectively, while 94.8 %, 94.7 %, 80 % and 100 % respectively in VITEK 2.

CA between RAST and SAST is 86.9 %, 95.3 %, 100 %, and 82.3 % respectively with BD Phoenix, while 91.8 %, 91.9 %, 85.7 %, and 84.6 % respectively with VITEK 2, on the same group of bacteria. The VITEK 2 automated system showed consistency of results with >90 % CA suggesting high reliability for both direct and routine AST in Gram-negative bacteria.

Conclusion

VITEK 2 demonstrated consistently high reliability for both direct and routine AST in Gram-negative bacteria. The BD phoenix showed high reliability for direct AST only, across all organisms tested. Direct AST protocol established in this study is simple, cost-effective, reliable, and rapid techniques reducing turnaround time (TAT) by 24 h and potentially improving patient's outcome.

目的：本研究旨在比较评估BD Phoenix和VITEK 2系统对阳性血培养瓶中ESKAPE分离株进行直接、常规和标准药敏试验（AST）的可靠性，主要关注将结果解释为敏感、中间或耐药类别。方法：对128株ESKAPE阳性血培养瓶进行直接AST、常规AST和标准AST检测，直接AST （DAST）和常规AST （RAST）分别采用BD Phoenix和VITEK 2自动化系统进行检测。DAST使用直接从阳性血液培养中获得的细菌颗粒进行，而RAST使用传代培养后的菌落进行。根据分类一致（CA）和差异对两者的结果进行比较。RAST结果进一步与标准AST （SAST）进行评估，通过Kirby-Bauer圆盘扩散试验，使用生长24小时的菌落。结果：采用BD Phoenix自动检测系统对肠杆菌、非发酵菌和革兰氏阳性球菌（金黄色葡萄球菌和肠球菌）的AST分类一致性（CA）分别为95.3%、100%和100%，而VITEK 2的DAST分类一致性分别为94.8%、94.7%、80%和100%。在同一组细菌上，BD Phoenix与SAST的CA分别为86.9%、95.3%、100%和82.3%，VITEK 2的CA分别为91.8%、91.9%、85.7%和84.6%。VITEK 2自动化系统显示结果的一致性为>90% CA，表明在革兰氏阴性菌中直接和常规AST的高可靠性。结论：VITEK 2在革兰氏阴性菌的直接和常规AST检测中均表现出一贯的高可靠性。在所有测试的生物中，BD凤凰仅对直接AST显示出高可靠性。本研究建立的直接AST协议是一种简单、经济、可靠和快速的技术，可将周转时间（TAT）缩短24小时。并且有可能改善病人的治疗效果。

{"title":"Evaluation of BD Phoenix and VITEK 2 for direct and routine antimicrobial susceptibility from positive blood culture bottles","authors":"Naima Sirad , Mustafa Altay Atalay , Pınar Sağıroğlu","doi":"10.1016/j.ijmmb.2025.100906","DOIUrl":"10.1016/j.ijmmb.2025.100906","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aims to comparatively evaluate the reliability of BD Phoenix and VITEK 2 systems for direct, routine and standard antimicrobial susceptibility testing (AST) of ESKAPE isolates from positive blood culture bottles, with a primary focus on the interpretation of results into susceptible, intermediate, or resistant categories.</div></div><div><h3>Methods</h3><div>A total of 128 ESKAPE isolates from positive blood culture bottles were subjected to direct, routine and standard AST. Direct AST (DAST) and routine AST (RAST) were performed using BD Phoenix and VITEK 2 automated systems. DAST was conducted using a bacterial pellet obtained directly from positive blood cultures, while RAST used a colony after subculture. Results of both were compared based on categorical agreement (CA) and discrepancies. RAST results were further evaluated against standard AST (SAST), performed via the Kirby-Bauer disc diffusion assay using 24 h-grown colonies.</div></div><div><h3>Findings and results</h3><div>The AST categorical agreements (CA) of DAST with the RAST using BD Phoenix automated system for Enterobacterales, Non-fermenting, and Gram-positive cocci (<em>S. aureus</em> and <em>Enterococci</em> spp.) were 95.3 %, 100 %, and 100 % respectively, while 94.8 %, 94.7 %, 80 % and 100 % respectively in VITEK 2.</div><div>CA between RAST and SAST is 86.9 %, 95.3 %, 100 %, and 82.3 % respectively with BD Phoenix, while 91.8 %, 91.9 %, 85.7 %, and 84.6 % respectively with VITEK 2, on the same group of bacteria. The VITEK 2 automated system showed consistency of results with >90 % CA suggesting high reliability for both direct and routine AST in Gram-negative bacteria.</div></div><div><h3>Conclusion</h3><div>VITEK 2 demonstrated consistently high reliability for both direct and routine AST in Gram-negative bacteria. The BD phoenix showed high reliability for direct AST only, across all organisms tested. Direct AST protocol established in this study is simple, cost-effective, reliable, and rapid techniques reducing turnaround time (TAT) by 24 h and potentially improving patient's outcome.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"56 ","pages":"Article 100906"},"PeriodicalIF":1.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular and genomic characterization of Leptospira isolates in humans and its relation to disease severity 人类钩端螺旋体分离株的分子和基因组特征及其与疾病严重程度的关系。

IF 1.4 4区医学 Q4 IMMUNOLOGY

Indian Journal of Medical Microbiology

Pub Date : 2025-07-01 DOI: 10.1016/j.ijmmb.2025.100910

Anitha Thattamparambil Ravindranathan , Aiswarya Mukundan , Sithara Nasar Thottathil Puthanpurayil , Bijesh Kavuthodi , Sunitha Karunakaran , Reena John

Objective

The study was conducted with the aim to find out the genomo species and serovars of Leptospira by gene sequencing among blood samples tested positive for Leptospira PCR and to identify the species associated with highest mortality.

Methods

All samples with Ct value less than 35 were included in the study. After lipL32 PCR amplification and agarose gel electrophoresis, the amplified products were subjected to gene sequencing. Demographic details and mortality data were obtained from records.

Results

66 out of the 70 samples showed maximum similarity to L.interrogans in sequencing. Other species identified include L.kirschneri and L. noguchi (5 samples). Among those tested positive, 14 patients died. L.interrogans was frequently isolated (13/14)from those who died.

The different serovars identified include serovar canicola/hardjo/copenhageni, manilae/bataviaea/lai/icterohaemorrhagiae. Of these, serovars identified in death include Serovar canicola/hardjo/copenhageni/ranaram/manila.

Conclusion

Following sequencing, we identified the predominant species as L. interrogans (91 %) with case fatality rate of 19 %. They are found in animal population of our area, pointing the zoonotic potential of Leptospira. This highlights the need for sensitization of those with animal exposure regarding preventive strategies.

目的：通过钩端螺旋体PCR检测阳性血液样本的基因测序，了解钩端螺旋体的基因组种类和血清型。我们还想知道死亡率最高的物种。方法：所有Ct值小于35的样本均纳入研究。琼脂糖凝胶电泳扩增后，对扩增产物进行基因测序。从记录中获得人口统计细节和死亡率数据。结果：70份样品中有66份在序列上与l .审问菌最相似。其他鉴定种包括L.kirschneri和L. noguchi（5个样本）。在检测呈阳性的患者中，有14人死亡。从死亡患者中分离出l .询问者较多（13/14）。已确定的不同血清型包括canicola/hardjo/copenhageni血清型、manilae/bataviaea /lai/黄疸出血热血清型。在死亡病例中发现的这些血清型包括canicola血清型/hardjo血清型/哥本哈根血清型/拉纳拉姆血清型/马尼拉血清型。结论：经测序鉴定，优势种为疑问乳杆菌（91%），病死率为19%。在我区动物种群中均有发现，提示钩端螺旋体有人畜共患的可能。这突出表明需要使所有接触动物的人对预防策略敏感。

{"title":"Molecular and genomic characterization of Leptospira isolates in humans and its relation to disease severity","authors":"Anitha Thattamparambil Ravindranathan , Aiswarya Mukundan , Sithara Nasar Thottathil Puthanpurayil , Bijesh Kavuthodi , Sunitha Karunakaran , Reena John","doi":"10.1016/j.ijmmb.2025.100910","DOIUrl":"10.1016/j.ijmmb.2025.100910","url":null,"abstract":"<div><h3>Objective</h3><div>The study was conducted with the aim to find out the genomo species and serovars of <em>Leptospira</em> by gene sequencing among blood samples tested positive for <em>Leptospira</em> PCR and to identify the species associated with highest mortality.</div></div><div><h3>Methods</h3><div>All samples with Ct value less than 35 were included in the study. After <em>lipL32 PCR</em> amplification and agarose gel electrophoresis, the amplified products were subjected to gene sequencing. Demographic details and mortality data were obtained from records.</div></div><div><h3>Results</h3><div>66 out of the 70 samples showed maximum similarity to <em>L.interrogans</em> in sequencing. Other species identified include <em>L</em>.<em>kirschneri</em> and <em>L. noguchi</em> (5 samples). Among those tested positive, 14 patients died. <em>L.interrogans</em> was frequently isolated (13/14)from those who died.</div><div>The different serovars identified include serovar <em>canicola</em>/<em>hardjo</em>/<em>copenhageni</em>, <em>manilae/bataviaea/lai/icterohaemorrhagiae</em>. Of these, serovars identified in death include Serovar <em>canicola/hardjo/copenhageni/ranaram/manila</em>.</div></div><div><h3>Conclusion</h3><div>Following sequencing, we identified the predominant species as <em>L. interrogans</em> (91 %) with case fatality rate of 19 %. They are found in animal population of our area, pointing the zoonotic potential of <em>Leptospira</em>. This highlights the need for sensitization of those with animal exposure regarding preventive strategies.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"56 ","pages":"Article 100910"},"PeriodicalIF":1.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144553416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic sexually acquired reactive arthritis, associated with extragenital Mycoplasma genitalium infection in an MSM – a case report 慢性性获得性反应性关节炎，与生殖器外支原体感染在男男性接触者-一个病例报告。

IF 1.4 4区医学 Q4 IMMUNOLOGY

Indian Journal of Medical Microbiology

Pub Date : 2025-07-01 DOI: 10.1016/j.ijmmb.2025.100899

Arkendu Basu , Jaya Biswas , Rajat Choudhary , Benu Dhawan

Reactive arthritis is included in the spectrum of seronegative spondyloarthritides, occurring secondary to triggers of genitourinary and gastrointestinal tract infections. We describe a case of sexually acquired reactive arthritis in an MSM, associated with extragenital Mycoplasma genitalium infection. Mycoplasma genitalium was detected from rectal swab by Real-Time PCR assays.

反应性关节炎是血清阴性脊柱关节炎的一种，继发于泌尿生殖系统和胃肠道感染。我们描述了一例性获得性反应性关节炎在男男性接触者，与生殖器支原体感染有关。采用实时荧光定量PCR法检测直肠拭子中生殖道支原体。

引用次数: 0

Aims and Scope 目标及范围

IF 1.3 4区医学 Q4 IMMUNOLOGY

Indian Journal of Medical Microbiology

Pub Date : 2025-07-01 DOI: 10.1016/S0255-0857(25)00142-2

引用次数: 0

Unveiling mumps prevalence: A cluster sampling approach in Dibrugarh, Assam 揭示流行性腮腺炎流行：阿萨姆邦迪布鲁加尔的整群抽样方法。

IF 1.4 4区医学 Q4 IMMUNOLOGY

Indian Journal of Medical Microbiology

Pub Date : 2025-07-01 DOI: 10.1016/j.ijmmb.2025.100911

Kimmi Sarmah , Kishore Sarma , Krishnarjun Bora , Nabajyoti Gogoi , Prasanta Kumar Borah , Ajanta Sharma , Jagadish Mahanta , Biswajyoti Borkakoty

Purpose

A serological survey was carried out for assessing mumps immunity in Dibrugarh, Assam, India using previously collected blood serum samples.

Methods

The study examined the prevalence of mumps-specific IgG in blood serum antibodies across various demographic groups, including different age groups, genders, and urban versus rural populations.

Results

The findings indicated a generally high level of immunity within the population. However, a significant proportion of children under 15 lacked these protective antibodies, highlighting their vulnerability to mumps infection.

Conclusion

This underscores the need for further studies to understand the factors influencing immunity patterns in the region. Additionally, it raises concerns about whether integrating the mumps vaccine into India's national immunization schedule could enhance protection, particularly for children. These insights contribute to a better understanding of mumps epidemiology in Dibrugarh and can guide future vaccination strategies to prevent outbreaks and protect public health.

目的：利用以前收集的血清样本，在印度阿萨姆邦迪布鲁加尔进行了血清学调查，以评估腮腺炎免疫力。方法：该研究检查了不同人口统计群体血清抗体中流行性腮腺炎特异性IgG的流行情况，包括不同年龄组、性别和城市与农村人口。结果：调查结果表明，在人群中普遍具有较高的免疫水平。然而，很大一部分15岁以下儿童缺乏这些保护性抗体，这突出了他们对腮腺炎感染的脆弱性。结论：这表明需要进一步研究了解影响该地区免疫模式的因素。此外，它引起了人们的关注，即将腮腺炎疫苗纳入印度的国家免疫计划是否可以加强保护，特别是对儿童的保护。这些见解有助于更好地了解迪布鲁加尔省的流行性腮腺炎流行病学，并可指导未来预防疫情和保护公众健康的疫苗接种战略。

{"title":"Unveiling mumps prevalence: A cluster sampling approach in Dibrugarh, Assam","authors":"Kimmi Sarmah , Kishore Sarma , Krishnarjun Bora , Nabajyoti Gogoi , Prasanta Kumar Borah , Ajanta Sharma , Jagadish Mahanta , Biswajyoti Borkakoty","doi":"10.1016/j.ijmmb.2025.100911","DOIUrl":"10.1016/j.ijmmb.2025.100911","url":null,"abstract":"<div><h3>Purpose</h3><div>A serological survey was carried out for assessing mumps immunity in Dibrugarh, Assam, India using previously collected blood serum samples.</div></div><div><h3>Methods</h3><div>The study examined the prevalence of mumps-specific IgG in blood serum antibodies across various demographic groups, including different age groups, genders, and urban versus rural populations.</div></div><div><h3>Results</h3><div>The findings indicated a generally high level of immunity within the population. However, a significant proportion of children under 15 lacked these protective antibodies, highlighting their vulnerability to mumps infection.</div></div><div><h3>Conclusion</h3><div>This underscores the need for further studies to understand the factors influencing immunity patterns in the region. Additionally, it raises concerns about whether integrating the mumps vaccine into India's national immunization schedule could enhance protection, particularly for children. These insights contribute to a better understanding of mumps epidemiology in Dibrugarh and can guide future vaccination strategies to prevent outbreaks and protect public health.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"57 ","pages":"Article 100911"},"PeriodicalIF":1.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occult hepatitis B virus infection among chronic liver disease patients: Insights from a tertiary care hospital 慢性肝病患者隐匿性乙型肝炎病毒感染：来自三级医院的见解。

IF 1.4 4区医学 Q4 IMMUNOLOGY

Indian Journal of Medical Microbiology

Pub Date : 2025-07-01 DOI: 10.1016/j.ijmmb.2025.100903

S.R. Ramya , Shashikala Nair , S. Veeraraghavan , Reba Kanungo

Purpose

Occult hepatitis B virus (HBV) infection (OBI) is a often overlooked contributor to chronic liver disease (CLD). This study aimed to determine the occurrence of OBI among CLD patients, assessing the presence of HBV DNA in HBsAg-negative individuals and exploring associated risk factors and clinical implications.

Methods

A cross-sectional study was conducted at the Pondicherry Institute of Medical Sciences, enrolling 169 CLD patients who tested negative for HBsAg. Blood samples were collected and analyzed for anti-HBc antibodies using the VIDAS anti-HBc total ELFA kit (BIOMERIEUX). HBV DNA was detected using real-time PCR with the Qiagen Artus HBV PCR kit. Demographic, clinical, and laboratory data were recorded to identify risk factors and disease patterns.

Results

A total of 169 HBsAg negative chronic liver disease (CLD) patients were evaluated. Anti-HBc positivity was observed in 32 % (54/169), OBI was identified in 5.3 % (9 out of 169) of patients, characterized by concurrent presence of anti-HBc antibodies and detectable HBV DNA. OBI cases were exclusively male and primarily in 41–60-year age group. Clinical features and biochemical parameters did not differ significantly between OBI and non-OBI groups. Blood transfusion history was the only risk factor significantly associated with OBI (p = 0.04). HBV DNA-positive patients showed elevated liver enzymes, indicating ongoing hepatic injury.

Conclusions

Occult hepatitis B infection (OBI) was detected in 5.3 % of HBsAg-negative chronic liver disease (CLD) patients, primarily among middle-aged males. The findings emphasize the limitations of routine serological testing and advocate for the inclusion of anti HBc screening and HBV DNA testing, particularly in patients with prior blood transfusions. Early identification of OBI is crucial for appropriate clinical management and may help prevent further progression of liver disease in this high-risk group.

目的：隐匿性乙型肝炎病毒（HBV）感染（OBI）是慢性肝病（CLD）的一个经常被忽视的因素。本研究旨在确定CLD患者中OBI的发生，评估hbsag阴性个体中HBV DNA的存在，并探讨相关的危险因素和临床意义。方法：在本地治里医学科学研究所进行了一项横断面研究，招募了169名HBsAg阴性的CLD患者。采集血样并使用VIDAS抗hbc总ELFA试剂盒（BIOMERIEUX）分析抗hbc抗体。采用Qiagen Artus HBV PCR试剂盒实时PCR检测HBV DNA。记录了人口统计学、临床和实验室数据，以确定危险因素和疾病模式。结果：共对169例HBsAg阴性的慢性肝病（CLD）患者进行评估。抗hbc阳性患者占32% (54/169)，OBI患者占5.3%(169 / 9)，其特征是同时存在抗hbc抗体和可检测的HBV DNA。OBI病例全部为男性，主要发生在41-60岁年龄组。临床特征和生化指标在OBI组和非OBI组之间无显著差异。输血史是唯一与OBI显著相关的危险因素（p = 0.04）。HBV dna阳性患者显示肝酶升高，表明肝损伤正在进行。结论：隐匿性乙型肝炎感染（OBI）在5.3%的hbsag阴性慢性肝病（CLD）患者中检测到，主要发生在中年男性中。研究结果强调了常规血清学检测的局限性，并提倡纳入抗HBc筛查和HBV DNA检测，特别是在有输血史的患者中。早期识别OBI对于适当的临床管理至关重要，并可能有助于预防这一高危人群肝脏疾病的进一步发展。

{"title":"Occult hepatitis B virus infection among chronic liver disease patients: Insights from a tertiary care hospital","authors":"S.R. Ramya , Shashikala Nair , S. Veeraraghavan , Reba Kanungo","doi":"10.1016/j.ijmmb.2025.100903","DOIUrl":"10.1016/j.ijmmb.2025.100903","url":null,"abstract":"<div><h3>Purpose</h3><div>Occult hepatitis B virus (HBV) infection (OBI) is a often overlooked contributor to chronic liver disease (CLD). This study aimed to determine the occurrence of OBI among CLD patients, assessing the presence of HBV DNA in HBsAg-negative individuals and exploring associated risk factors and clinical implications.</div></div><div><h3>Methods</h3><div>A cross-sectional study was conducted at the Pondicherry Institute of Medical Sciences, enrolling 169 CLD patients who tested negative for HBsAg. Blood samples were collected and analyzed for anti-HBc antibodies using the VIDAS anti-HBc total ELFA kit (BIOMERIEUX). HBV DNA was detected using real-time PCR with the Qiagen Artus HBV PCR kit. Demographic, clinical, and laboratory data were recorded to identify risk factors and disease patterns.</div></div><div><h3>Results</h3><div>A total of 169 HBsAg negative chronic liver disease (CLD) patients were evaluated. Anti-HBc positivity was observed in 32 % (54/169), OBI was identified in 5.3 % (9 out of 169) of patients, characterized by concurrent presence of anti-HBc antibodies and detectable HBV DNA. OBI cases were exclusively male and primarily in 41–60-year age group. Clinical features and biochemical parameters did not differ significantly between OBI and non-OBI groups. Blood transfusion history was the only risk factor significantly associated with OBI (p = 0.04). HBV DNA-positive patients showed elevated liver enzymes, indicating ongoing hepatic injury.</div></div><div><h3>Conclusions</h3><div>Occult hepatitis B infection (OBI) was detected in 5.3 % of HBsAg-negative chronic liver disease (CLD) patients, primarily among middle-aged males. The findings emphasize the limitations of routine serological testing and advocate for the inclusion of anti HBc screening and HBV DNA testing, particularly in patients with prior blood transfusions. Early identification of OBI is crucial for appropriate clinical management and may help prevent further progression of liver disease in this high-risk group.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"56 ","pages":"Article 100903"},"PeriodicalIF":1.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144475000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seroprevalence of spotted fever group rickettsiosis in Kashmir: A community-based study using indirect immunofluorescence assay 克什米尔斑疹热组立克次体病的血清患病率：一项使用间接免疫荧光法的社区研究

IF 1.4 4区医学 Q4 IMMUNOLOGY

Indian Journal of Medical Microbiology

Pub Date : 2025-06-22 DOI: 10.1016/j.ijmmb.2025.100902

Bashir Fomda , Uksim Qadri , Rauf Ur Rashid , Sehrish Baqal , Qounsar Nisar

Background and objectives

Rickettsial infections, caused by obligate intracellular Gram-negative bacteria, are an emerging public health concern globally, particularly in regions like India, where the epidemiology remains largely underexplored. The study aimed to determine the seroprevalence of spotted fever rickettsiosis (SFR) in the Kashmir Valley, using the indirect immunofluorescence assay (IFA), the gold standard for rickettsial diagnosis.

Materials and methods

This prospective, population-based study was conducted over three years at the Sher-i-Kashmir Institute of Medical Sciences, Srinagar. A multistage random sampling technique was used and a total of 571 individuals across 10 districts were included in the study. Blood samples were analysed for SFR IgG antibodies using IFA. Data was collected through a house-to-house survey, including demographic and environmental risk factors. Statistical analysis was performed using SPSS version 23, with significance set at P < 0.05.

Results

Of the 571 participants, 143 (25 %) tested positive by IgG immunofluorescence assay for SFR. The majority of seropositive cases were males, 52.4 % (n = 75). Seropositivity was highest in the >50 year age group and among participants having risk factors like contact with uncut vegetations, collection of Firewood And living near Forests. The seroprevalence varied significantly across districts, with the highest prevalence observed in Pulwama. Risk factors associated with higher seropositivity included contact with shrubs, milking and animal slaughtering. No significant associations were found between gender, urban/rural residence, or age group and seropositivity.

Conclusion

This study highlights a high seroprevalence of spotted fever in the Kashmir Valley, emphasizing the significant public health burden in the region. Early detection and targeted prevention strategies, particularly in high-risk areas, are crucial for effective control and management of spotted fever in this region.

背景和目的由专性细胞内革兰氏阴性菌引起的立克次体感染是全球新兴的公共卫生问题，特别是在印度等流行病学尚未充分研究的地区。该研究旨在利用间接免疫荧光法（IFA）确定克什米尔山谷斑点热立克次体病（SFR）的血清患病率，这是立克次体诊断的金标准。材料和方法这项前瞻性的、以人群为基础的研究是在斯利那加的Sher-i-Kashmir医学科学研究所进行的。采用多阶段随机抽样方法，共对10个地区的571名个体进行了调查。用IFA分析血样中SFR IgG抗体。数据是通过挨家挨户的调查收集的，包括人口和环境风险因素。采用SPSS version 23进行统计学分析，显著性设置为P <；0.05.结果571例参与者中，143例（25%）SFR IgG免疫荧光检测阳性。血清阳性病例以男性为主，占52.4% （n = 75）。血清阳性反应在50岁年龄组和有接触未砍伐植被、收集柴火和居住在森林附近等危险因素的参与者中最高。不同地区的血清患病率差异很大，普尔瓦马的患病率最高。与较高血清阳性相关的危险因素包括与灌木接触、挤奶和动物屠宰。性别、城市/农村居住地或年龄组与血清阳性之间未发现显著关联。结论本研究强调了克什米尔谷地斑点热的高血清流行率，强调了该地区重大的公共卫生负担。早期发现和有针对性的预防战略，特别是在高风险地区，对于有效控制和管理本地区的斑点热至关重要。

{"title":"Seroprevalence of spotted fever group rickettsiosis in Kashmir: A community-based study using indirect immunofluorescence assay","authors":"Bashir Fomda , Uksim Qadri , Rauf Ur Rashid , Sehrish Baqal , Qounsar Nisar","doi":"10.1016/j.ijmmb.2025.100902","DOIUrl":"10.1016/j.ijmmb.2025.100902","url":null,"abstract":"<div><h3>Background and objectives</h3><div>Rickettsial infections, caused by obligate intracellular Gram-negative bacteria, are an emerging public health concern globally, particularly in regions like India, where the epidemiology remains largely underexplored. The study aimed to determine the seroprevalence of spotted fever rickettsiosis (SFR) in the Kashmir Valley, using the indirect immunofluorescence assay (IFA), the gold standard for rickettsial diagnosis.</div></div><div><h3>Materials and methods</h3><div>This prospective, population-based study was conducted over three years at the Sher-i-Kashmir Institute of Medical Sciences, Srinagar. A multistage random sampling technique was used and a total of 571 individuals across 10 districts were included in the study. Blood samples were analysed for SFR IgG antibodies using IFA. Data was collected through a house-to-house survey, including demographic and environmental risk factors. Statistical analysis was performed using SPSS version 23, with significance set at P < 0.05.</div></div><div><h3>Results</h3><div>Of the 571 participants, 143 (25 %) tested positive by IgG immunofluorescence assay for SFR. The majority of seropositive cases were males, 52.4 % (n = 75). Seropositivity was highest in the >50 year age group and among participants having risk factors like contact with uncut vegetations, collection of Firewood And living near Forests. The seroprevalence varied significantly across districts, with the highest prevalence observed in Pulwama. Risk factors associated with higher seropositivity included contact with shrubs, milking and animal slaughtering. No significant associations were found between gender, urban/rural residence, or age group and seropositivity.</div></div><div><h3>Conclusion</h3><div>This study highlights a high seroprevalence of spotted fever in the Kashmir Valley, emphasizing the significant public health burden in the region. Early detection and targeted prevention strategies, particularly in high-risk areas, are crucial for effective control and management of spotted fever in this region.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"56 ","pages":"Article 100902"},"PeriodicalIF":1.4,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144481457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microsporidial keratoconjunctivitis in an immunocompetent adolescent 免疫功能正常青少年的微孢子性角膜结膜炎

IF 1.4 4区医学 Q4 IMMUNOLOGY

Indian Journal of Medical Microbiology

Pub Date : 2025-06-21 DOI: 10.1016/j.ijmmb.2025.100898

Debasish Biswal , Rashmi Mittal , Ananya Arora , Sadia Khan , Anubhav Pandey , Sharmila Sengupta

引用次数: 0

Neurological involvement in two phases of Kyasanur Forest Disease 恰萨努尔森林病两个阶段的神经系统参与。

IF 1.4 4区医学 Q4 IMMUNOLOGY

Indian Journal of Medical Microbiology

Pub Date : 2025-06-20 DOI: 10.1016/j.ijmmb.2025.100901

Pothumarthy Venkata Swathikiran , Nitin Gupta , Muralidhar Varma , Chiranjay Mukhopdhyay

Kyasanur Forest Disease (KFD) is a tick-borne viral illness endemic to Southern India, often categorized as viral haemorrhagic fever, though its biphasic neurological involvement is less frequently discussed. We present the case of an elderly woman from Karnataka, India, who initially presented with fever and altered sensorium, diagnosed with the first phase of KFD and treated with supportive care for ten days. Four weeks later, she returned with second-phase manifestations of fever, headache, neck stiffness and lymphocytic pleocytosis on cerebrospinal fluid analysis. We report this case to emphasize the need for high suspicion of KFD in patients presenting with neurological symptoms in the endemic regions.

Kyasanur森林病（KFD）是一种在印度南部流行的蜱传病毒性疾病，通常被归类为病毒性出血热，尽管其双期神经系统累及较少被讨论。我们报告一位来自印度卡纳塔克邦的老年妇女的病例，她最初表现为发烧和感觉改变，被诊断为KFD的第一阶段，并接受了十天的支持性护理。4周后，患者以发热、头痛、颈部僵硬和脑脊液淋巴细胞增多症为第二期症状返回。我们报告这个病例是为了强调在流行地区出现神经系统症状的患者需要高度怀疑KFD。

引用次数: 0

Advancing STI diagnostics – Potential of aptasensors as POCTs STI诊断技术的进步——适体传感器作为poct的潜力

IF 1.4 4区医学 Q4 IMMUNOLOGY

Indian Journal of Medical Microbiology

Pub Date : 2025-06-20 DOI: 10.1016/j.ijmmb.2025.100900

Shilpi Malhotra , Rachna Verma, Seema Sood

引用次数: 0

首页上一页

类型

全部化学•材料生命科学医学物理工程技术环境•农林材料科学地球科学法学管理学化学环境科学与生态学计算机科学教育学经济学农林科学人文科学生物学数学物理与天体物理心理学综合性期刊其他工业工程理学历史学农学文学信息工程

数据库

全部 ACS Publications Elsevier ieeexplore Springer The Royal Society of Chemistry Wiley

期刊

Indian Journal of Medical Microbiology

全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.

﹀