Pub Date : 2025-11-01Epub Date: 2025-09-03DOI: 10.1016/j.ijmmb.2025.100957
Lydia Jennifer Sumanth , Rajiv Karthik , Anuradha Chandramohan , Aparna Irodi , Rani D. Sahni , Joy Sarojini Michael
Lomentospora prolificans (formerly Scedosporium prolificans) is an emerging fungal pathogen, affecting both immunocompromised and immunocompetent individuals. Treatment is difficult due to intrinsic resistance against multiple anti-fungal agents. We describe five patients with L. prolificans infections attending a tertiary care center in South India. Out of the 5 patients, 4 patients presented with deep seated infection, and 1 with disseminated disease. Risk factors included uncontrolled diabetes mellitus, advanced HIV infection, T cell lymphoblastic leukemia, carcinoma breast, and immunosuppressant therapy. L. prolificans as an important causative agent of deep seated and disseminated mycoses among immunocompromised patients.
{"title":"Microbiological and Clinical Epidemiology of Lomentospora prolificans infections","authors":"Lydia Jennifer Sumanth , Rajiv Karthik , Anuradha Chandramohan , Aparna Irodi , Rani D. Sahni , Joy Sarojini Michael","doi":"10.1016/j.ijmmb.2025.100957","DOIUrl":"10.1016/j.ijmmb.2025.100957","url":null,"abstract":"<div><div><em>Lomentospora prolificans</em> (formerly <em>Scedosporium prolificans</em>) is an emerging fungal pathogen, affecting both immunocompromised and immunocompetent individuals. Treatment is difficult due to intrinsic resistance against multiple anti-fungal agents. We describe five patients with <em>L. prolificans</em> infections attending a tertiary care center in South India. Out of the 5 patients, 4 patients presented with deep seated infection, and 1 with disseminated disease. Risk factors included uncontrolled diabetes mellitus, advanced HIV infection, T cell lymphoblastic leukemia, carcinoma breast, and immunosuppressant therapy. <em>L. prolificans</em> as an important causative agent of deep seated and disseminated mycoses among immunocompromised patients.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"58 ","pages":"Article 100957"},"PeriodicalIF":1.3,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145006081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We report a case of phaeohyphomycotic cyst due to Parathyridaria percutanea in a 40-year-old post renal transplant patient from India. He presented with a painless swelling between the 2nd and 3rd toes of his left foot after minor trauma to the foot with a brick. The lesion was treated by complete surgical excision without any recurrence. The fungus was identified by sequencing of internal transcribed spacer (ITS) region of rDNA. Parathyridaria percutanea infections in immunocompromised patients of Indian and African origin warrants determination of host and environmental factors.
{"title":"Parathyridaria percutanea causing phaeohyphomycotic cyst in a renal transplant patient: Is it an emerging pathogen?","authors":"Harsimran Kaur , Haseen Ahmad , Karthick Kumar , Radhika Srinivasan , Ashish Sharma , Tarun Narang , Jasmine Sethi , Sourav Agnihotri , Shivaprakash M. Rudramurthy","doi":"10.1016/j.ijmmb.2025.100985","DOIUrl":"10.1016/j.ijmmb.2025.100985","url":null,"abstract":"<div><div>We report a case of phaeohyphomycotic cyst due to <em>Parathyridaria percutanea</em> in a 40-year-old post renal transplant patient from India. He presented with a painless swelling between the 2nd and 3rd toes of his left foot after minor trauma to the foot with a brick. The lesion was treated by complete surgical excision without any recurrence. The fungus was identified by sequencing of internal transcribed spacer (ITS) region of rDNA. <em>Parathyridaria percutanea</em> infections in immunocompromised patients of Indian and African origin warrants determination of host and environmental factors.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"58 ","pages":"Article 100985"},"PeriodicalIF":1.3,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145174861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The incidence of candidemia due to non-albicans Candida is increasing worldwide in various surveillance studies. Gastrointestinal tract is considered as one of the portal of entry for potential sources of invasive infections. Necrotizing Enterocolitis (NEC) is a serious inflammatory condition of gastrointestinal tract in preterm infants. Here, we describe a case of NEC in a neonate associated with an invasive infection caused by the uncommon non-albicans Candida species, Candida guilliermondii.
{"title":"Candida guilliermondii candidemia: A rare finding in a neonate with necrotizing enterocolitis","authors":"Shweta Singh , Vivek Hada , Anchala Bharadwaj , Gaurav Gupta , Shubhangi Chaturvedi , Atul R. Rukadikar , Aroop Mohanty , Parul Singh","doi":"10.1016/j.ijmmb.2025.100969","DOIUrl":"10.1016/j.ijmmb.2025.100969","url":null,"abstract":"<div><div>The incidence of candidemia due to non-<em>albicans Candida</em> is increasing worldwide in various surveillance studies. Gastrointestinal tract is considered as one of the portal of entry for potential sources of invasive infections. Necrotizing Enterocolitis (NEC) is a serious inflammatory condition of gastrointestinal tract in preterm infants. Here, we describe a case of NEC in a neonate associated with an invasive infection caused by the uncommon non-<em>albicans Candida</em> species, <em>Candida guilliermondii</em>.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"58 ","pages":"Article 100969"},"PeriodicalIF":1.3,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145058390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pulmonary invasive fungal infections (IFIs), particularly among immunocompromised individuals, are associated with substantial morbidity and mortality. This study aimed to evaluate the clinical profile, underlying risk factors, and diagnostic performance of the galactomannan (GM) antigen assay in the diagnosis of Invasive Pulmonary Aspergillosis (IPA).
Material and methods
A cross-sectional observational study was conducted over 12 months at the Himalayan Institute of Medical Sciences, Dehradun, India. A total of 224 patients with suspected pulmonary IFIs were enrolled. Cases were classified as Proven, Probable, Possible, or No IFI based on the EORTC/MSG, Invasive Fungal Diseases in Adult Patients in Intensive Care Unit (FUNDICU) criteria. Respiratory and serum samples were analysed using direct potassium hydroxide (KOH) microscopy, fungal culture, and GM antigen detection via lateral flow assay. Diagnostic accuracy was determined using standard statistical analyses.
Results
Of the 224 patients, 46.4 % were classified as Proven or Probable IFI, 34.8 % as Possible, and 18.8 % as No IFI. dyspnoea (p = 0.002) was the most strongly associated symptom. Significant risk factors included asthma/COPD (52.9 %, p = 0.001), diabetes mellitus (36.5 %, p = 0.028), immunosuppression (37.5 %, p < 0.001). Fungal culture was positive in 30.3 % of cases, with Aspergillus flavus and A. fumigatus as the predominant isolates. A bronchoalveolar lavage fluid (BALF) GM optical density index (ODI) cut-off of ≥1.0 achieved balanced sensitivity (55.56 %) and specificity (75.86 %).
Conclusions
The GM assay in BALF at an ODI threshold of ≥1.0 showsmodest discriminative ability for the early detection of invasive pulmonary aspergillosis (IPA). Incorporating GM testing alongside conventional diagnostics enhances early identification and facilitates prompt antifungal therapy.
{"title":"Invasive pulmonary aspergillosis (IPA)- A study of risk factors, diagnostic modalities and role of galactomannan antigen detection","authors":"Mani Bhushan Pant , Rajender Singh , Garima Mittal , Rakhee Khanduri","doi":"10.1016/j.ijmmb.2025.100982","DOIUrl":"10.1016/j.ijmmb.2025.100982","url":null,"abstract":"<div><h3>Introduction</h3><div>Pulmonary invasive fungal infections (IFIs), particularly among immunocompromised individuals, are associated with substantial morbidity and mortality. This study aimed to evaluate the clinical profile, underlying risk factors, and diagnostic performance of the galactomannan (GM) antigen assay in the diagnosis of Invasive Pulmonary Aspergillosis (IPA).</div></div><div><h3>Material and methods</h3><div>A cross-sectional observational study was conducted over 12 months at the Himalayan Institute of Medical Sciences, Dehradun, India. A total of 224 patients with suspected pulmonary IFIs were enrolled. Cases were classified as Proven, Probable, Possible, or No IFI based on the EORTC/MSG, Invasive Fungal Diseases in Adult Patients in Intensive Care Unit (FUNDICU) criteria. Respiratory and serum samples were analysed using direct potassium hydroxide (KOH) microscopy, fungal culture, and GM antigen detection via lateral flow assay. Diagnostic accuracy was determined using standard statistical analyses.</div></div><div><h3>Results</h3><div>Of the 224 patients, 46.4 % were classified as Proven or Probable IFI, 34.8 % as Possible, and 18.8 % as No IFI. dyspnoea (<em>p</em> = 0.002) was the most strongly associated symptom. Significant risk factors included asthma/COPD (52.9 %, <em>p</em> = 0.001), diabetes mellitus (36.5 %, p = 0.028), immunosuppression (37.5 %, <em>p</em> < 0.001). Fungal culture was positive in 30.3 % of cases, with <em>Aspergillus flavus</em> and <em>A. fumigatus</em> as the predominant isolates. A bronchoalveolar lavage fluid (BALF) GM optical density index (ODI) cut-off of ≥1.0 achieved balanced sensitivity (55.56 %) and specificity (75.86 %).</div></div><div><h3>Conclusions</h3><div>The GM assay in BALF at an ODI threshold of ≥1.0 showsmodest discriminative ability for the early detection of invasive pulmonary aspergillosis (IPA). Incorporating GM testing alongside conventional diagnostics enhances early identification and facilitates prompt antifungal therapy.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"58 ","pages":"Article 100982"},"PeriodicalIF":1.3,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Extended-spectrum beta-lactamases (ESBLs), particularly in Enterobacterales, are major contributors to the growing problem of antibiotic resistance in Gram-negative bacilli. This guidance document provides an overview of the epidemiology, identification, and clinical management of infections caused by Extended-Spectrum Beta-Lactamase-producing Enterobacterales (ESBL-E). This guideline adds on the previously issued Indian Council of Medical Research (ICMR) guidelines on carbapenem-resistant organisms (CROs) and intended to support clinicians in making evidence-based decisions regarding the diagnosis and treatment of ESBL infections, thereby promoting effective patient management and antimicrobial stewardship.
Objectives
The purpose of this guidance document aims to assist clinicians in selecting not only the right antibiotics to diagnose ESBLs, but also right tests to diagnose ESBLs, for effective management of ESBL infections.
Content
This guidance document highlights the importance of early and accurate identification of ESBL -producing Enterobacterales, outlines clinical syndromes that may require empirical antibiotic coverage for ESBLs, and offers guidance on appropriate de-escalation strategies. Additionally, it emphasizes on the optimal use of use of newer beta-lactam/beta-lactamase inhibitor (BLBLI) combinations, such as cefepime-enmetazobactam and ceftolozane-tazobactam, and also emphasizes on avoiding important drugs like ceftazidime-avibactam for ESBL -producing Enterobacterales.
{"title":"Management of infections caused by Extended-spectrum beta-lactamase-producing Enterobacterales in Indian patients","authors":"Veeraraghavan Balaji , Nitin Bansal , Ram Gopalakrishnan , Camilla Rodrigues , V. Ramasubramanian , George M. Varghese , Vasant Nagvekar , Pallab Ray , Sanjay Bhattacharya , Neha Gupta , Priscilla Rupali , Rajalakshmi Ananthanarayanan , Sonam Vijay , Kamini Walia","doi":"10.1016/j.ijmmb.2025.100974","DOIUrl":"10.1016/j.ijmmb.2025.100974","url":null,"abstract":"<div><h3>Background</h3><div>Extended-spectrum beta-lactamases (ESBLs), particularly in Enterobacterales, are major contributors to the growing problem of antibiotic resistance in Gram-negative bacilli. This guidance document provides an overview of the epidemiology, identification, and clinical management of infections caused by Extended-Spectrum Beta-Lactamase-producing Enterobacterales (ESBL-E). This guideline adds on the previously issued Indian Council of Medical Research (ICMR) guidelines on carbapenem-resistant organisms (CROs) and intended to support clinicians in making evidence-based decisions regarding the diagnosis and treatment of ESBL infections, thereby promoting effective patient management and antimicrobial stewardship.</div></div><div><h3>Objectives</h3><div>The purpose of this guidance document aims to assist clinicians in selecting not only the right antibiotics to diagnose ESBLs, but also right tests to diagnose ESBLs, for effective management of ESBL infections.</div></div><div><h3>Content</h3><div>This guidance document highlights the importance of early and accurate identification of ESBL -producing Enterobacterales<em>,</em> outlines clinical syndromes that may require empirical antibiotic coverage for ESBLs, and offers guidance on appropriate de-escalation strategies. Additionally, it emphasizes on the optimal use of use of newer beta-lactam/beta-lactamase inhibitor (BLBLI) combinations, such as cefepime-enmetazobactam and ceftolozane-tazobactam, and also emphasizes on avoiding important drugs like ceftazidime-avibactam for ESBL -producing Enterobacterales.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"58 ","pages":"Article 100974"},"PeriodicalIF":1.3,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145086038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-01Epub Date: 2025-10-25DOI: 10.1016/j.ijmmb.2025.101004
G. Vithiya , D.T. Rajendran , M. Srividya , M. Shagana , Shobana Devi , M. Pavithra , R. Sivaganesa Karthikeyan , R. Haribalaganesh
Environmental surveillance of Burkholderia pseudomallei in India is limited, with sparse data from non-coastal regions. This study aimed to detect B. pseudomallei in soil collected near the residences of culture-confirmed melioidosis patients in southern India using molecular methods. A total of 50 soil samples were collected from a depth of 30 cm around the homes of five melioidosis patients in Sivagangai district, Tamil Nadu. Soil DNA was extracted and analyzed using conventional PCR targeting the TTS1 gene cluster of B. pseudomallei. Two(4 %) of 50 soil samples tested were positive for B. pseudomallei by PCR.
{"title":"Identification of Burkholderia pseudomallei in the environment of patients with melioidosis in Tamil Nadu, India","authors":"G. Vithiya , D.T. Rajendran , M. Srividya , M. Shagana , Shobana Devi , M. Pavithra , R. Sivaganesa Karthikeyan , R. Haribalaganesh","doi":"10.1016/j.ijmmb.2025.101004","DOIUrl":"10.1016/j.ijmmb.2025.101004","url":null,"abstract":"<div><div>Environmental surveillance of <em>Burkholderia pseudomallei</em> in India is limited, with sparse data from non-coastal regions. This study aimed to detect <em>B. pseudomallei</em> in soil collected near the residences of culture-confirmed melioidosis patients in southern India using molecular methods. A total of 50 soil samples were collected from a depth of 30 cm around the homes of five melioidosis patients in Sivagangai district, Tamil Nadu. Soil DNA was extracted and analyzed using conventional PCR targeting the TTS1 gene cluster of <em>B. pseudomallei</em>. Two(4 %) of 50 soil samples tested were positive for <em>B. pseudomallei</em> by PCR.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"58 ","pages":"Article 101004"},"PeriodicalIF":1.3,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145516323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-01Epub Date: 2025-09-13DOI: 10.1016/j.ijmmb.2025.100971
Sridevi Dinakaran , Patricia Anitha K. , Ajit R. Sawant , Sheela Devi Chandrakesan , Reba Kanungo
Purpose
Infections caused by multidrug-resistant Acinetobacter baumannii (MDR-AB) are a major global problem. Increasing resistance to carbapenems among Acinetobacter baumannii (A.baumannii) has led to using colistin as a last resort. Overuse of colistin will lead to the emergence of colistin resistance as evidenced in the past with other antibiotics. In India, reports of colistin-resistant A.baumannii are emerging. The mutations in the pmrA/pmrB genes may induce colistin resistance. However, increasing the minimum inhibitory concentration (MIC) of colistin and detection of mutations in the pmrB gene may provide information on the possibility of emerging resistance.
Methods
A total of 50 clinical isolates MDR-AB were subjected to colistin broth microdilution and colistin challenge test. Amongst them, five isolates that had slightly higher MIC were sequenced to detect mutations.
Results
Out of 50 isolates, 24 % were isolated from patients who sustained trauma due to road traffic accidents and the majority of them (96 %) required ventilator support. Twenty-seven (56 %) isolates were from patients who developed ventilator-associated pneumonia. The mortality rate was highest (71.4 %) among sepsis cases. The colistin MIC was in the susceptible range in all the isolates. Amongst the five isolates that had slightly higher MIC, only one had a mutation in the pmrB gene.
Conclusion
Due to antibiotic pressure, A.baumannii can develop resistance to colistin through genomic mutation. If this trend continues, colistin therapy will become ineffective. This can cause serious implications in the management, especially in critically ill patients. Through a stringent antimicrobial stewardship program (AMSP) the spread of resistance could be controlled.
{"title":"Screening for mutations in pmrB gene to predict potential colistin resistance among the clinical isolates of multidrug-resistant Acinetobacter baumannii","authors":"Sridevi Dinakaran , Patricia Anitha K. , Ajit R. Sawant , Sheela Devi Chandrakesan , Reba Kanungo","doi":"10.1016/j.ijmmb.2025.100971","DOIUrl":"10.1016/j.ijmmb.2025.100971","url":null,"abstract":"<div><h3>Purpose</h3><div>Infections caused by multidrug-resistant <em>Acinetobacter baumannii</em> (MDR-AB) are a major global problem. Increasing resistance to carbapenems among <em>Acinetobacter baumannii</em> (<em>A.baumannii</em>) has led to using colistin as a last resort. Overuse of colistin will lead to the emergence of colistin resistance as evidenced in the past with other antibiotics. In India, reports of colistin-resistant <em>A.baumannii</em> are emerging. The mutations in the <em>pmrA/pmrB</em> genes may induce colistin resistance. However, increasing the minimum inhibitory concentration (MIC) of colistin and detection of mutations in the <em>pmrB</em> gene may provide information on the possibility of emerging resistance.</div></div><div><h3>Methods</h3><div>A total of 50 clinical isolates MDR-AB were subjected to colistin broth microdilution and colistin challenge test. Amongst them, five isolates that had slightly higher MIC were sequenced to detect mutations.</div></div><div><h3>Results</h3><div>Out of 50 isolates, 24 % were isolated from patients who sustained trauma due to road traffic accidents and the majority of them (96 %) required ventilator support. Twenty-seven (56 %) isolates were from patients who developed ventilator-associated pneumonia. The mortality rate was highest (71.4 %) among sepsis cases. The colistin MIC was in the susceptible range in all the isolates. Amongst the five isolates that had slightly higher MIC, only one had a mutation in the <em>pmrB</em> gene.</div></div><div><h3>Conclusion</h3><div>Due to antibiotic pressure, <em>A.baumannii</em> can develop resistance to colistin through genomic mutation. If this trend continues, colistin therapy will become ineffective. This can cause serious implications in the management, especially in critically ill patients. Through a stringent antimicrobial stewardship program (AMSP) the spread of resistance could be controlled.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"58 ","pages":"Article 100971"},"PeriodicalIF":1.3,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145069420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Although Aeromonas dhakensis has recently been reported as an important human pathogen, it is still less frequently identified in comparison to Aeromonas hydrophila or Aeromonas caviae, due to the problem of identification in certain automated systems.
This case report provides an impetus to identify and report this organism as soon as possible. Further studies on this organism's virulence and pathogenic mechanism, especially in healthcare settings, are warranted.
{"title":"Aeromonas dhakensis - A red alert in blood cultures!","authors":"Shobha Prasada , Ethel Suman , Pooja Rao , Swaraj Dutta , Sathish B. Rao , Suchitra Shenoy","doi":"10.1016/j.ijmmb.2025.101002","DOIUrl":"10.1016/j.ijmmb.2025.101002","url":null,"abstract":"<div><div>Although <em>Aeromonas dhakensis</em> has recently been reported as an important human pathogen, it is still less frequently identified in comparison to <em>Aeromonas hydrophila</em> or <em>Aeromonas caviae</em>, due to the problem of identification in certain automated systems.</div><div>This case report provides an impetus to identify and report this organism as soon as possible. Further studies on this organism's virulence and pathogenic mechanism, especially in healthcare settings, are warranted.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"58 ","pages":"Article 101002"},"PeriodicalIF":1.3,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145320494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-01Epub Date: 2025-08-07DOI: 10.1016/j.ijmmb.2025.100942
Vijayan Priya, S. Nagarathna, Kumari HB. Veena
{"title":"Corrigendum to “Molecular characterization of methicillin-resistant Staphylococcus aureus: Dissemination of multidrug-resistant community-associated MRSA and emergence of LA-MRSA, in a healthcare setting” [Indian J Med Microbiol 54 (2025) 100810]","authors":"Vijayan Priya, S. Nagarathna, Kumari HB. Veena","doi":"10.1016/j.ijmmb.2025.100942","DOIUrl":"10.1016/j.ijmmb.2025.100942","url":null,"abstract":"","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"58 ","pages":"Article 100942"},"PeriodicalIF":1.3,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144798942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aims to identify the distribution of different clostridial species in human infections, sample types, and their antimicrobial susceptibility profile using the E-test method.
Materials and methods
The samples were processed as per the standard bacteriological techniques. Automated anaerobic gas evacuation-replacement system was used to create anaerobiosis (85 % N2, 10 % CO2, 5 % H2). Antibiotic susceptibility of isolated Clostridium species was performed using gradient diffusion (E strip) method against metronidazole, clindamycin, and penicillin.
Results
On antibiotic susceptibility testing by E-test, we have found 96.77 % susceptibility to metronidazole, 90.32 % susceptibility to penicillin, and 74.19 % susceptibility to clindamycin.
Conclusion
It can be inferred that the E-test, when performed with the right inoculum, and when given the adequate period of incubation, could be used to reliably perform AST in Clostridium species. It produces a reliable report within 96 h of sample processing.
{"title":"Antibiotic susceptibility profile of clinical isolates of Clostridium species from a tertiary care hospital in North India: A prospective study","authors":"Manharpreet Kaur , Anjali Anil , Mani Bhushan Kumar , Cherring Tandup , Pallab Ray , Archana Angrup","doi":"10.1016/j.ijmmb.2025.100981","DOIUrl":"10.1016/j.ijmmb.2025.100981","url":null,"abstract":"<div><h3>Purpose</h3><div>This study aims to identify the distribution of different clostridial species in human infections, sample types, and their antimicrobial susceptibility profile using the E-test method.</div></div><div><h3>Materials and methods</h3><div>The samples were processed as per the standard bacteriological techniques. Automated anaerobic gas evacuation-replacement system was used to create anaerobiosis (85 % N<sub>2</sub>, 10 % CO<sub>2</sub>, 5 % H<sub>2</sub>). Antibiotic susceptibility of isolated <em>Clostridium</em> species was performed using gradient diffusion (E strip) method against metronidazole, clindamycin, and penicillin.</div></div><div><h3>Results</h3><div>On antibiotic susceptibility testing by E-test, we have found 96.77 % susceptibility to metronidazole, 90.32 % susceptibility to penicillin, and 74.19 % susceptibility to clindamycin.</div></div><div><h3>Conclusion</h3><div>It can be inferred that the E-test, when performed with the right inoculum, and when given the adequate period of incubation, could be used to reliably perform AST in <em>Clostridium</em> species. It produces a reliable report within 96 h of sample processing.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"58 ","pages":"Article 100981"},"PeriodicalIF":1.3,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145148729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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