Pea(Pisum sativum L.)is the second most important legume crop worldwide after chickpea (Cicer arietinum L.) and valuable resources for their genetic improvement. This study aimed to analyze genetic diversity of pea cultivars through morphological and molecular markers. The present investigation was carried out with 12 pea cultivars using 28 simple sequence repeat markers. A total of 60 polymorphic bands with an average of 2.31 bands per primer were obtained. The polymorphic information content, diversity index and resolving power were ranged from 0.50 to 0.33, 0.61 to 0.86 and 0.44 to 1.0 with an average of 0.46, 0.73 and 0.76, respectively. The 12 pea cultivars were grouped into 3 clusters obtained from cluster analysis with a Jaccardd’s similarity coefficient range of 0.47-0.78, indicating the sufficient genetic divergence among these cultivars of pea. The principal component analysis showed that first three principal components explained 86.97% of the total variation, suggesting the contribution of quantitative traits in genetic variability. The contribution of 32.59% for number of seeds per plant, stem circumference, number of pods per plant and number of seeds per pod in the PC1 leads to the conclusion that these traits contribute more to the total variation observed in the 12 pea cultivars and would make a good parental stock material. Overall, this SSR analysis complements morphological characters of initial selection of these pea germplasms for future breeding program.
Expansion of new paddy land for rice in the tidal area is still experiencing problems, especially their toxic concentrations of Fe which can affect soil productivity. Efforts to improve the productivity of the newly opened paddy fields of tidal swampland are with drainage and specific nutrient management. The purpose of this research is an effort to increase the productivity of land through drainage management and application of ameliorant against iron toxicity in rice at the newly opened tidal swampland. The experimental design used was a Randomized Block Design is repeated 5 times. The results showed that technology of 1 week drying and 1 week inundation, limed 2 t ha-1 and fertilized N 112.5 kg ha-1, P2O5 22.5 kg ha-1, K2O 67.5 kg ha-1 and organic materials by 5 t ha-1, can increase rice yields by 237% from 1.40 t ha-1 to 4.72 t ha-1 of dry milled grain and can reduce 50% of Fe in the soil content of 384 ppm to 192 ppm in the newly opened paddy fields of tidal swampland in dry season. While the rainy season, technology of 1 week drying and 2 week inundation, limed 2 t ha-1 and fertilized of N 86.25 kg ha-1, P2O5 30 kg ha-1, K2O 15 kg ha-1, and organic materials by 5 t ha-1, can increase rice yield 272 % from 1.21 t ha-1 to 4.50 t ha-1 of dry milled grain and can reduce 51.88% of Fe in the soil content of 1,168 ppm to 769 ppm.
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：