Pub Date : 2015-04-01DOI: 10.21082/ijas.v16n1.2015.p21-30
E. Surmaini, T. Hadi, K. Subagyono, N. Puspito
Indonesia consistently experiences dry climatic conditions and droughts during El Nino, with significant consequences for rice production. To mitigate the impacts of such droughts, robust, simple and timely rainfall forecast is critically important for predicting drought prior to planting time over rice growing areas in Indonesia. The main objective of this study was to predict drought in rice growing areas using ensemble seasonal prediction. The skill of National Oceanic and Atmospheric Administration’s (NOAA’s) seasonal prediction model Climate Forecast System version 2 (CFSv2) for predicting rice drought in West Java was investigated in a series of hindcast experiments in 1989-2010. The Constructed Analogue (CA) method was employed to produce downscaled local rainfall prediction with stream function (y) and velocity potential (c) at 850 hPa as predictors and observed rainfall as predictant. We used forty two rain gauges in northern part of West Java in Indramayu, Cirebon, Sumedang and Majalengka Districts. To be able to quantify the uncertainties, a multi-window scheme for predictors was applied to obtain ensemble rainfall prediction. Drought events in dry season planting were predicted by rainfall thresholds. The skill of downscaled rainfall prediction was assessed using Relative Operating Characteristics (ROC) method. Results of the study showed that the skills of the probabilistic seasonal prediction for early detection of rice area drought were found to range from 62% to 82% with an improved lead time of 2-4 months. The lead time of 2-4 months provided sufficient time for practical policy makers, extension workers and farmers to cope with drought by preparing suitable farming practices and equipments.
{"title":"PREDICTION OF DROUGHT IMPACT ON RICE PADDIES IN WEST JAVA USING ANALOGUE DOWNSCALING METHOD","authors":"E. Surmaini, T. Hadi, K. Subagyono, N. Puspito","doi":"10.21082/ijas.v16n1.2015.p21-30","DOIUrl":"https://doi.org/10.21082/ijas.v16n1.2015.p21-30","url":null,"abstract":"Indonesia consistently experiences dry climatic conditions and droughts during El Nino, with significant consequences for rice production. To mitigate the impacts of such droughts, robust, simple and timely rainfall forecast is critically important for predicting drought prior to planting time over rice growing areas in Indonesia. The main objective of this study was to predict drought in rice growing areas using ensemble seasonal prediction. The skill of National Oceanic and Atmospheric Administration’s (NOAA’s) seasonal prediction model Climate Forecast System version 2 (CFSv2) for predicting rice drought in West Java was investigated in a series of hindcast experiments in 1989-2010. The Constructed Analogue (CA) method was employed to produce downscaled local rainfall prediction with stream function (y) and velocity potential (c) at 850 hPa as predictors and observed rainfall as predictant. We used forty two rain gauges in northern part of West Java in Indramayu, Cirebon, Sumedang and Majalengka Districts. To be able to quantify the uncertainties, a multi-window scheme for predictors was applied to obtain ensemble rainfall prediction. Drought events in dry season planting were predicted by rainfall thresholds. The skill of downscaled rainfall prediction was assessed using Relative Operating Characteristics (ROC) method. Results of the study showed that the skills of the probabilistic seasonal prediction for early detection of rice area drought were found to range from 62% to 82% with an improved lead time of 2-4 months. The lead time of 2-4 months provided sufficient time for practical policy makers, extension workers and farmers to cope with drought by preparing suitable farming practices and equipments.","PeriodicalId":13456,"journal":{"name":"Indonesian Journal of Agricultural Science","volume":"16 1","pages":"21-30"},"PeriodicalIF":0.0,"publicationDate":"2015-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67800487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-04-01DOI: 10.21082/ijas.v16n1.2015.p39-50
D. Susilowati, I. Sudiana, N. R. Mubarik, A. Suwanto
Rhizobacteria are important components of soil and directly or indirectly influence the soils quality and plant growth for maintaining adequate plant nutrition and reducing the negative environmental effects of fertilizers. Applying high dose of chemical fertilizers in most of rice fields in the coastal areas could reduce the quality of the soil in the long time. There are few studies addressed to verify the species and functional diversity of cultivable rhizobacteria associated with rice plant in the coastal soils. The objective of the study was to verify the species and functional diversity of rhizobacteria isolated from the coastal soils of two rice production areas of Subang and Indramayu, West Java. Special focus was given to verify phosphate solubilization, nitrogen fixation, IAA and cellulase production of the selected 78 strains of rice rhizobacteria isolated from the coastal paddy field, as well as taxonomical analyses based on 16S rRNA. The results showed that among 78 bacterial isolates from the coastal paddy field, mostly were belonging to the Firmicutes, most of them affiliated with genera Bacillus, 75 strains produced IAA, 32 strains fixed nitrogen, 37 strains solubilized phosphate and 33 strains produced cellulase. Several strains of the rhizobacteria were capable of producing plant growth promoting substances (PGPR), alone or in combination, such as IAA, fixing nitrogen, solubilizing phosphate, and producing cellulase. Taking all of these diverse PGPR characteristics into account, it is clear that the 78 identified isolates have great potential for improving saline soils of the coastal paddy fields in Indonesia.
{"title":"SPECIES AND FUNCTIONAL DIVERSITY OF RHIZOBACTERIA OF RICE PLANT IN THE COASTAL SOILS OF INDONESIA","authors":"D. Susilowati, I. Sudiana, N. R. Mubarik, A. Suwanto","doi":"10.21082/ijas.v16n1.2015.p39-50","DOIUrl":"https://doi.org/10.21082/ijas.v16n1.2015.p39-50","url":null,"abstract":"Rhizobacteria are important components of soil and directly or indirectly influence the soils quality and plant growth for maintaining adequate plant nutrition and reducing the negative environmental effects of fertilizers. Applying high dose of chemical fertilizers in most of rice fields in the coastal areas could reduce the quality of the soil in the long time. There are few studies addressed to verify the species and functional diversity of cultivable rhizobacteria associated with rice plant in the coastal soils. The objective of the study was to verify the species and functional diversity of rhizobacteria isolated from the coastal soils of two rice production areas of Subang and Indramayu, West Java. Special focus was given to verify phosphate solubilization, nitrogen fixation, IAA and cellulase production of the selected 78 strains of rice rhizobacteria isolated from the coastal paddy field, as well as taxonomical analyses based on 16S rRNA. The results showed that among 78 bacterial isolates from the coastal paddy field, mostly were belonging to the Firmicutes, most of them affiliated with genera Bacillus, 75 strains produced IAA, 32 strains fixed nitrogen, 37 strains solubilized phosphate and 33 strains produced cellulase. Several strains of the rhizobacteria were capable of producing plant growth promoting substances (PGPR), alone or in combination, such as IAA, fixing nitrogen, solubilizing phosphate, and producing cellulase. Taking all of these diverse PGPR characteristics into account, it is clear that the 78 identified isolates have great potential for improving saline soils of the coastal paddy fields in Indonesia.","PeriodicalId":13456,"journal":{"name":"Indonesian Journal of Agricultural Science","volume":"16 1","pages":"39-50"},"PeriodicalIF":0.0,"publicationDate":"2015-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67800534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-04-01DOI: 10.21082/ijas.v16n1.2015.p31-38
Kusdianawati Kusdianawati, A. Z. Mustopa, S. Suharsono, B. Budiarto, F. Fatimah, Hasim Danuri
Plantaricin is one of bacteriocins that have the potential to be used as food preservative. Plantaricin is safe for human consumption because it can be easily degraded by proteolytic enzymes. The objective of this study was to express and purify recombinant pre-mature peptide of plantaricin F from Lactobacillus plantarum S34 in Escherichia coli . Plantaricin gene-specific primer was used to obtain pln F structural gene amplicon from L. plantarum S34. This amplicon was cloned in pET32a vector and expressed in E. coli BL21 (DE3) pLysS. Pre-mature plantaricin F peptide was expressed as Histagged-fusion protein and separated by Co2+-chelating affinity chromatography. L. plantarum S34-derived pre-mature plantaricin F peptide fused with thioredoxin-(His)6tag had successfully been expressed in E. coli BL21 (DE3) pLysS using pET32a as an expression vector. The fused recombinant pln F as pre-mature state expressed had a molecular mass of +24 kDa, meanwhile the fused recombinant that contained only the leader peptide of pln F appeared as +20 kDa based on SDS-PAGE separations. The optimal production of fused recombinant pln F as soluble fraction was obtained when culture condition was added with 0.5 mM of IPTG and incubated at 22°C for 5 hours (OD~1). Furthermore, the expression of fused recombinant pln F as its pre-mature peptide pointed out that the pln F’s leader peptide could be proteolytically cleaved by a system in heterologous cells. Overall, heterologous pln F production as pre-mature peptide fused with thioredoxin-(His)6tag had been well established. From this research, we expect plantaricin F can be expressed and purified in E. coli.
{"title":"Construction, Expression and Purification of Recombinant Pre-mature Peptide of Plantaricin F From Lactobacillus Plantarum S34 in Escherichia Coli","authors":"Kusdianawati Kusdianawati, A. Z. Mustopa, S. Suharsono, B. Budiarto, F. Fatimah, Hasim Danuri","doi":"10.21082/ijas.v16n1.2015.p31-38","DOIUrl":"https://doi.org/10.21082/ijas.v16n1.2015.p31-38","url":null,"abstract":"Plantaricin is one of bacteriocins that have the potential to be used as food preservative. Plantaricin is safe for human consumption because it can be easily degraded by proteolytic enzymes. The objective of this study was to express and purify recombinant pre-mature peptide of plantaricin F from Lactobacillus plantarum S34 in Escherichia coli . Plantaricin gene-specific primer was used to obtain pln F structural gene amplicon from L. plantarum S34. This amplicon was cloned in pET32a vector and expressed in E. coli BL21 (DE3) pLysS. Pre-mature plantaricin F peptide was expressed as Histagged-fusion protein and separated by Co2+-chelating affinity chromatography. L. plantarum S34-derived pre-mature plantaricin F peptide fused with thioredoxin-(His)6tag had successfully been expressed in E. coli BL21 (DE3) pLysS using pET32a as an expression vector. The fused recombinant pln F as pre-mature state expressed had a molecular mass of +24 kDa, meanwhile the fused recombinant that contained only the leader peptide of pln F appeared as +20 kDa based on SDS-PAGE separations. The optimal production of fused recombinant pln F as soluble fraction was obtained when culture condition was added with 0.5 mM of IPTG and incubated at 22°C for 5 hours (OD~1). Furthermore, the expression of fused recombinant pln F as its pre-mature peptide pointed out that the pln F’s leader peptide could be proteolytically cleaved by a system in heterologous cells. Overall, heterologous pln F production as pre-mature peptide fused with thioredoxin-(His)6tag had been well established. From this research, we expect plantaricin F can be expressed and purified in E. coli.","PeriodicalId":13456,"journal":{"name":"Indonesian Journal of Agricultural Science","volume":"16 1","pages":"31-38"},"PeriodicalIF":0.0,"publicationDate":"2015-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67800524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-10-13DOI: 10.21082/ijas.v15n2.2014.p71-78
N. F. Devy, Y. Yenni, Hardiyanto Hardiyanto
Somatic embryogenesis (SE) of callus culture in vitro is one of citrus propagation ways for producing free virus and genetically true-to-type plantlets. To induce growing of plantlets derived from this technology, they should be grafted ex vitro onto a citrus rootstock. The research aimed to evaluate the growth performance of citrus plants cv. Siam Kintamani (Citrus nobilis L.) that used both plantlets and scions as their stocks. The research was conducted at Tlekung Research Station, Indonesian Citrus and Subtropical Fruit Research Institute from June 2011 to December 2012. The treatments were done at nursery house by grafting a plantlet and budding a scion onto an eight-month-old Japanese Citroon (JC) rootstock plant. The grafted and budded plants of one-year old were maintained at nursery house then transplanted into the field. In the field, the research was arranged in a randomized block design with three replications and used 15 plants as unit samples. The results showed that the vegetative growth of Siam Kintamani seedling derived from SE or grafted plant was faster than that of budded plant started from 10 to 12 months after treatment in the nersery house. In the field, the growth of SE grafted plant was only significantly different up to 6 months after transplanting. Plantlets produced from SE in vitro propagation can be used as a good alternative stock material for producing healthy citrus plants. Therefore, a further research is required especially on varieties used, reproductive growth and massive planlets production.
柑桔愈伤组织体外培养的体细胞胚发生是柑桔获得游离病毒和遗传真型植株的一种繁殖方式。为了诱导该技术衍生的植株生长，应将其离体嫁接到柑橘砧木上。本研究旨在评价柑橘属植物cv的生长性能。以植株和接穗为砧木的Siam Kintamani (Citrus nobilis L.)。研究于2011年6月至2012年12月在印度尼西亚柑橘和亚热带水果研究所Tlekung研究站进行。这些处理是在苗圃里进行的，通过嫁接一株植物并在一株8个月大的日本雪铁龙(JC)砧木上发芽。一年生嫁接和出芽植株在苗圃养护后移栽到田间。田间研究采用3个重复的随机区组设计，以15株植物为单位样本。结果表明:在苗圃处理10 ~ 12个月后，由SE和嫁接植株衍生的暹罗金塔马尼幼苗的营养生长速度快于出芽植株。在田间，SE嫁接植株的生长仅在移栽后6个月有显著差异。SE离体繁殖植株可作为柑桔健康植株的良好替代砧木材料。因此，尤其在品种利用、生殖生长和大苗生产等方面还需进一步研究。
{"title":"THE GROWTH PERFORMANCE OF CITRUS DERIVED FROM SOMATIC EMBRYOGENESIS PLANTLET AND SCION STOCK","authors":"N. F. Devy, Y. Yenni, Hardiyanto Hardiyanto","doi":"10.21082/ijas.v15n2.2014.p71-78","DOIUrl":"https://doi.org/10.21082/ijas.v15n2.2014.p71-78","url":null,"abstract":"Somatic embryogenesis (SE) of callus culture in vitro is one of citrus propagation ways for producing free virus and genetically true-to-type plantlets. To induce growing of plantlets derived from this technology, they should be grafted ex vitro onto a citrus rootstock. The research aimed to evaluate the growth performance of citrus plants cv. Siam Kintamani (Citrus nobilis L.) that used both plantlets and scions as their stocks. The research was conducted at Tlekung Research Station, Indonesian Citrus and Subtropical Fruit Research Institute from June 2011 to December 2012. The treatments were done at nursery house by grafting a plantlet and budding a scion onto an eight-month-old Japanese Citroon (JC) rootstock plant. The grafted and budded plants of one-year old were maintained at nursery house then transplanted into the field. In the field, the research was arranged in a randomized block design with three replications and used 15 plants as unit samples. The results showed that the vegetative growth of Siam Kintamani seedling derived from SE or grafted plant was faster than that of budded plant started from 10 to 12 months after treatment in the nersery house. In the field, the growth of SE grafted plant was only significantly different up to 6 months after transplanting. Plantlets produced from SE in vitro propagation can be used as a good alternative stock material for producing healthy citrus plants. Therefore, a further research is required especially on varieties used, reproductive growth and massive planlets production.","PeriodicalId":13456,"journal":{"name":"Indonesian Journal of Agricultural Science","volume":"15 1","pages":"71-78"},"PeriodicalIF":0.0,"publicationDate":"2014-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67800327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-10-13DOI: 10.21082/IJAS.V15N2.2014.P47-54
E. I. Riyanti, E. Listanto, A. D. Ambarwati
Late blight caused by Phytophthora infestans is an important disease on potato. Several potato hybrids have been generated by crossing local varieties (Atlantic and Granola) with Katahdin SP951 which contains late blight resistance gene RB. Prior to release, these hybrids need to be evaluated for their environ-mental effects on non-target organisms and natural pests and diseases. The objectives of the study were to investigate the effect of LBR potato hybrids on beneficial soil microbes, pests and diseases. The trial was conducted in the confined field trial (CFT) in Lembang, West Java. The parental non-transgenic (NT) clones (Granola, Atlantic and Katahdin) and LBR hybrids (four clones of Atlantic x Katahdin SP951 hybrids; 10 clones of Granola x Katahdin SP951) were planted at a plant spacing of 30 cm x 70 cm. Fungicide applications were used as treat-ments (no spray, five and twenty times sprays). The experi-ment was arranged in a randomized completely block design with three replications. The parameters determined were popula-tions of N2 fixing and P solubilizing bacteria, soil C/N ratio as well as natural pests and diseases. The results showed that the transgenic LBR potato hybrids did not have negative effect on N fixing bacteria. The bacterial populations were around 1010-11 cells g-1 soil before planting, 1012 cells at 1.5 months after planting (MAP) and 108 cells after harvest. For P- solubilizing bacteria, their populations were 1010 cells before planting, 1012 cells at 1.5 MAP and 1011 cells g-1 soil after harvest. The soil C/N ratio of the transgenic plot was not statistically different compared to non-transgenic plot, i.e. 12-15 before planting, 10-11 at 1.5 MAP, and 10 after harvest in non-spray plot. Pests and diseases such as Alternaria solani, Liriomyza, potato tubber moth, aphid and mites on the transgenic and non-transgenic plots were statistically not different. The resistance score for A. solani was 7.2 (parental tansgenic) and 7.6 (parental non-transgenic); for Liriomyza it was 2.07 (parental transgenic) and 2.32 insect per plant (parental non-transgenic), the PTM was 0.63 (parental transgenic) and 0.73 insect per plant (parental non-transgenic), aphid and mites were 0.75 (parental transgenic) and 1.68 insects per plant (parental non-transgenic). The study indicated that LBR potato hybrids did not have any negative impacts on non-target organisms.
马铃薯晚疫病是马铃薯的一种重要病害。将当地马铃薯品种(大西洋和格兰诺拉)与含有抗晚疫病基因RB的Katahdin SP951杂交，产生了几种马铃薯杂交种。在投放之前，需要评估这些杂交种对非目标生物和自然病虫害的环境影响。本研究旨在探讨LBR马铃薯杂交种对土壤有益微生物和病虫害的影响。该试验是在西爪哇Lembang的封闭田间试验(CFT)中进行的。亲本非转基因(NT)无性系(Granola、Atlantic和Katahdin)和LBR杂交种(Atlantic x Katahdin SP951杂交种4个无性系;以格兰诺拉×卡塔丁SP951无性系10个为试验材料，株距为30 cm × 70 cm。施用杀菌剂作为处理(不喷、喷5次和喷20次)。试验采用完全随机区组设计，设3个重复。测定的参数包括固氮和溶磷细菌的数量、土壤碳氮比和自然病虫害。结果表明，转基因LBR马铃薯杂交种对固氮细菌没有负面影响。种植前g-1土壤细菌数量约为1010-11个细胞，种植后1.5个月(MAP)细菌数量约为1012个细胞，收获后细菌数量约为108个细胞。对溶磷菌而言，种植前为1010个细胞，在1.5 MAP条件下为1012个细胞，收获后为1011个细胞。转基因地块与非转基因地块的土壤C/N比差异无统计学意义，种植前为12-15,1.5 MAP时为10-11，非喷施地块收获后为10。转基因地与非转基因地病虫害如番茄赤霉、绿蝇、马铃薯管蛾、蚜虫、螨虫等在转基因地与非转基因地间无统计学差异。抗性评分为7.2分(亲本转基因)和7.6分(亲本非转基因);蝇蛆的PTM分别为0.63(亲本转基因)和0.73(亲本非转基因)，蚜虫和螨虫的PTM分别为0.75(亲本转基因)和1.68(亲本非转基因)。研究表明，LBR马铃薯杂交种对非目标生物没有负面影响。
{"title":"EFFECTS OF LATE BLIGHT RESISTANT POTATO CONTAINING RB GENE ON THE SOIL MICROBES, PESTS AND PLANT DISEASES","authors":"E. I. Riyanti, E. Listanto, A. D. Ambarwati","doi":"10.21082/IJAS.V15N2.2014.P47-54","DOIUrl":"https://doi.org/10.21082/IJAS.V15N2.2014.P47-54","url":null,"abstract":"Late blight caused by Phytophthora infestans is an important disease on potato. Several potato hybrids have been generated by crossing local varieties (Atlantic and Granola) with Katahdin SP951 which contains late blight resistance gene RB. Prior to release, these hybrids need to be evaluated for their environ-mental effects on non-target organisms and natural pests and diseases. The objectives of the study were to investigate the effect of LBR potato hybrids on beneficial soil microbes, pests and diseases. The trial was conducted in the confined field trial (CFT) in Lembang, West Java. The parental non-transgenic (NT) clones (Granola, Atlantic and Katahdin) and LBR hybrids (four clones of Atlantic x Katahdin SP951 hybrids; 10 clones of Granola x Katahdin SP951) were planted at a plant spacing of 30 cm x 70 cm. Fungicide applications were used as treat-ments (no spray, five and twenty times sprays). The experi-ment was arranged in a randomized completely block design with three replications. The parameters determined were popula-tions of N2 fixing and P solubilizing bacteria, soil C/N ratio as well as natural pests and diseases. The results showed that the transgenic LBR potato hybrids did not have negative effect on N fixing bacteria. The bacterial populations were around 1010-11 cells g-1 soil before planting, 1012 cells at 1.5 months after planting (MAP) and 108 cells after harvest. For P- solubilizing bacteria, their populations were 1010 cells before planting, 1012 cells at 1.5 MAP and 1011 cells g-1 soil after harvest. The soil C/N ratio of the transgenic plot was not statistically different compared to non-transgenic plot, i.e. 12-15 before planting, 10-11 at 1.5 MAP, and 10 after harvest in non-spray plot. Pests and diseases such as Alternaria solani, Liriomyza, potato tubber moth, aphid and mites on the transgenic and non-transgenic plots were statistically not different. The resistance score for A. solani was 7.2 (parental tansgenic) and 7.6 (parental non-transgenic); for Liriomyza it was 2.07 (parental transgenic) and 2.32 insect per plant (parental non-transgenic), the PTM was 0.63 (parental transgenic) and 0.73 insect per plant (parental non-transgenic), aphid and mites were 0.75 (parental transgenic) and 1.68 insects per plant (parental non-transgenic). The study indicated that LBR potato hybrids did not have any negative impacts on non-target organisms.","PeriodicalId":13456,"journal":{"name":"Indonesian Journal of Agricultural Science","volume":"15 1","pages":"47-54"},"PeriodicalIF":0.0,"publicationDate":"2014-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67800797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-04-10DOI: 10.21082/IJAS.V15N1.2014.P1-10
N. Nurindah, D. Sunarto
Cotton production nationally is low due to various constraints, including pests. Two main pests commonly found in cotton plantation in rain fed fields are cotton leafhopper (Amrasca biguttula) and cotton bollworm (Helicoverpa armigera). The study aimed to evaluate four packages of integrated pest management (IPM) techniques to control cotton leafhopper and cotton bollworm in rain fed fields. The experiment was conducted in farmers’ fields at Asembagus, East Java, between January and July 2012. Four packages of IPM evaluated were cotton varieties, i.e. Kanesia 10 or Kanesia 13, and seed treatment with synthetic insecticide (imidacloprid) before sowing or spraying molasses (10 ml L-1 water) as food for natural enemies. The cotton plants were intercropped with groundnut and sprayed with neem seed extract (NSE) at the action threshold level for pest control. These packages were compared among themselves and also with the methods usually used by farmers, i.e. planting cotton variety Kanesia 8 intercropped with groundnut and pest control using synthetic chemical insecticides. Twenty five plants were sampled randomly per plot and measured for their growth, leafhopper and bollworm populations, as well as cotton seed yield per plot. Observations were made weekly, starting at 30 days after planting (DAP) until 120 DAP. The results showed that the use of Kanesia 10 or Kanesia 13 intercropped with groundnut and spraying molasses to conserve natural enemies was the best pest management practice and superior to farmers’ practices. Conserving natural enemies is not only profitable (saving production cost of IDR1,150,000 to IDR1,500,000 ha-1 season-1), but also safe for the environment (no need to spray chemical insecticides).
{"title":"EVALUATION OF FOUR INTEGRATED PEST MANAGEMENT PACKAGES FOR CONTROLLING MAIN PESTS OF COTTON IN RAINFED FIELDS","authors":"N. Nurindah, D. Sunarto","doi":"10.21082/IJAS.V15N1.2014.P1-10","DOIUrl":"https://doi.org/10.21082/IJAS.V15N1.2014.P1-10","url":null,"abstract":"Cotton production nationally is low due to various constraints, including pests. Two main pests commonly found in cotton plantation in rain fed fields are cotton leafhopper (Amrasca biguttula) and cotton bollworm (Helicoverpa armigera). The study aimed to evaluate four packages of integrated pest management (IPM) techniques to control cotton leafhopper and cotton bollworm in rain fed fields. The experiment was conducted in farmers’ fields at Asembagus, East Java, between January and July 2012. Four packages of IPM evaluated were cotton varieties, i.e. Kanesia 10 or Kanesia 13, and seed treatment with synthetic insecticide (imidacloprid) before sowing or spraying molasses (10 ml L-1 water) as food for natural enemies. The cotton plants were intercropped with groundnut and sprayed with neem seed extract (NSE) at the action threshold level for pest control. These packages were compared among themselves and also with the methods usually used by farmers, i.e. planting cotton variety Kanesia 8 intercropped with groundnut and pest control using synthetic chemical insecticides. Twenty five plants were sampled randomly per plot and measured for their growth, leafhopper and bollworm populations, as well as cotton seed yield per plot. Observations were made weekly, starting at 30 days after planting (DAP) until 120 DAP. The results showed that the use of Kanesia 10 or Kanesia 13 intercropped with groundnut and spraying molasses to conserve natural enemies was the best pest management practice and superior to farmers’ practices. Conserving natural enemies is not only profitable (saving production cost of IDR1,150,000 to IDR1,500,000 ha-1 season-1), but also safe for the environment (no need to spray chemical insecticides).","PeriodicalId":13456,"journal":{"name":"Indonesian Journal of Agricultural Science","volume":"15 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2014-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67799831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-04-10DOI: 10.21082/IJAS.V15N1.2014.P29-34
R. Harni, S. Supramana, S. Supriadi
Pratylenchus brachyurus is a major parasitic nematode on patchouli that reduces plant production up to 85%. The use of endophytic bacteria is promising for controlling nematode and promoting plant growth through production of phytohormones and enhancing the availability of soil nutrients. The objective of the study was to evaluate the efficacy of endophytic bacteria to control P. brachyurus on patchouli plant and its influence on plant productions (plant fresh weight and patchouli oil). The study was conducted at Cimanggu Experimental Garden and Laboratory of the Indonesian Spice and Medicinal Crops Research Institute (ISMECRI), Bogor, West Java. The experi-ment was designed in a randomized block with seven treatments and eight replications; each replication consisted of 10 plants. The treatments evaluated were five isolates of endophytic bacteria (Achromobacter xylosoxidans TT2, Alcaligenes faecalis NJ16, Pseudomonas putida EH11, Bacillus cereus MSK and Bacillus subtilis NJ57), synthetic nematicide as a reference, and non-treated plant as a control. Four-week old patchouli plants of cv. Sidikalang were treated by soaking the roots in suspension of endophytic bacteria (109 cfu ml-1) for one hour before trans-planting to the field. At one month after planting, the plants were drenched with the bacterial suspension as much as 100 ml per plant. The results showed that applications of the endophytic bacteria could suppress the nematode populations (52.8-80%) and increased plant weight (23.62-57.48%) compared to the control. The isolate of endophytic bacterium Achromobacter xylosoxidans TT2 was the best and comparable with carbofuran.
{"title":"EFFICACY OF ENDOPHYTIC BACTERIA IN REDUCING PLANT PARASITIC NEMATODE Pratylenchus brachyurus","authors":"R. Harni, S. Supramana, S. Supriadi","doi":"10.21082/IJAS.V15N1.2014.P29-34","DOIUrl":"https://doi.org/10.21082/IJAS.V15N1.2014.P29-34","url":null,"abstract":"Pratylenchus brachyurus is a major parasitic nematode on patchouli that reduces plant production up to 85%. The use of endophytic bacteria is promising for controlling nematode and promoting plant growth through production of phytohormones and enhancing the availability of soil nutrients. The objective of the study was to evaluate the efficacy of endophytic bacteria to control P. brachyurus on patchouli plant and its influence on plant productions (plant fresh weight and patchouli oil). The study was conducted at Cimanggu Experimental Garden and Laboratory of the Indonesian Spice and Medicinal Crops Research Institute (ISMECRI), Bogor, West Java. The experi-ment was designed in a randomized block with seven treatments and eight replications; each replication consisted of 10 plants. The treatments evaluated were five isolates of endophytic bacteria (Achromobacter xylosoxidans TT2, Alcaligenes faecalis NJ16, Pseudomonas putida EH11, Bacillus cereus MSK and Bacillus subtilis NJ57), synthetic nematicide as a reference, and non-treated plant as a control. Four-week old patchouli plants of cv. Sidikalang were treated by soaking the roots in suspension of endophytic bacteria (109 cfu ml-1) for one hour before trans-planting to the field. At one month after planting, the plants were drenched with the bacterial suspension as much as 100 ml per plant. The results showed that applications of the endophytic bacteria could suppress the nematode populations (52.8-80%) and increased plant weight (23.62-57.48%) compared to the control. The isolate of endophytic bacterium Achromobacter xylosoxidans TT2 was the best and comparable with carbofuran.","PeriodicalId":13456,"journal":{"name":"Indonesian Journal of Agricultural Science","volume":"15 1","pages":"29-34"},"PeriodicalIF":0.0,"publicationDate":"2014-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67799855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-04-10DOI: 10.21082/IJAS.V15N1.2014.P11-16
L. Hakim, Suyamto Suyamto, E. Paturohman
The F2 progenies of crosses among three soybean varieties were evaluated with their parents at Ngale Experimental Farm, Ngawi, East Java during the dry season of 2011. The experi-ment was conducted in a randomized block design with three replications. The seeds of each F2 progenies and parents were planted in two rows of 4.5 m long. Plant spacing was 40 cm x 15 cm and each hill contained one plant. Among the characters studied, seed weight per plant, number of pods per plant and days to maturity had the highest coefficient of variability with the means of 52.6%, 48.2% and 41.9%, respectively. Whereas seed size, plant height and number of reproductive nodes had moderate values of 36.9%, 35.5% and 37.4%, respectively. Number of branches and days to flowering had the lowest variability of 11.7% and 15.3%, respectively. Number of pods per plant, days to maturity, plant height and seed size had high mean estimates of heritability coupled with high genetic advance. The genetic progress of these characters that would be obtained for one generation of selection was estimated 47.2%, 45.7%, 40.9% and 40.3%, respectively. Among the agronomic characters, plant height, number of pods per plant, seed size and days to maturity had the highest coefficient of variability associated with high heritability and genetic advance. Therefore, selection for high yielding with early maturity soybean genotypes based on plant height, number of pods per plant and seed size might be possible.
2011年旱季，在东爪哇省恩加维的恩加勒实验农场，对三个大豆品种杂交的F2后代及其亲本进行了评价。试验采用随机区组设计，设3个重复。每个F2子代和亲本的种子排成两行，长4.5 m。株距为40 cm × 15 cm，每山一株。单株种子重、单株荚果数和成熟期变异系数最高，均值分别为52.6%、48.2%和41.9%。种子大小、株高和生殖节数中等，分别为36.9%、35.5%和37.4%。枝数和花期变异率最低，分别为11.7%和15.3%。单株荚果数、成熟天数、株高和种子大小具有较高的平均遗传力和较高的遗传进步。经一代选择，这些性状的遗传进步率分别为47.2%、45.7%、40.9%和40.3%。农艺性状中，株高、单株荚果数、种子大小和成熟期变异系数最高，具有较高的遗传力和遗传先进性。因此，根据株高、单株荚果数和种子大小选择高产早熟大豆基因型是可行的。
{"title":"GENETIC VARIABILITY, HERITABILITY AND EXPECTED GENETIC ADVANCES OF QUANTITATIVE CHARACTERS IN F2 PROGENIES OF SOYBEAN CROSSES","authors":"L. Hakim, Suyamto Suyamto, E. Paturohman","doi":"10.21082/IJAS.V15N1.2014.P11-16","DOIUrl":"https://doi.org/10.21082/IJAS.V15N1.2014.P11-16","url":null,"abstract":"The F2 progenies of crosses among three soybean varieties were evaluated with their parents at Ngale Experimental Farm, Ngawi, East Java during the dry season of 2011. The experi-ment was conducted in a randomized block design with three replications. The seeds of each F2 progenies and parents were planted in two rows of 4.5 m long. Plant spacing was 40 cm x 15 cm and each hill contained one plant. Among the characters studied, seed weight per plant, number of pods per plant and days to maturity had the highest coefficient of variability with the means of 52.6%, 48.2% and 41.9%, respectively. Whereas seed size, plant height and number of reproductive nodes had moderate values of 36.9%, 35.5% and 37.4%, respectively. Number of branches and days to flowering had the lowest variability of 11.7% and 15.3%, respectively. Number of pods per plant, days to maturity, plant height and seed size had high mean estimates of heritability coupled with high genetic advance. The genetic progress of these characters that would be obtained for one generation of selection was estimated 47.2%, 45.7%, 40.9% and 40.3%, respectively. Among the agronomic characters, plant height, number of pods per plant, seed size and days to maturity had the highest coefficient of variability associated with high heritability and genetic advance. Therefore, selection for high yielding with early maturity soybean genotypes based on plant height, number of pods per plant and seed size might be possible.","PeriodicalId":13456,"journal":{"name":"Indonesian Journal of Agricultural Science","volume":"15 1","pages":"11-16"},"PeriodicalIF":0.0,"publicationDate":"2014-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67799841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-04-10DOI: 10.21082/IJAS.V15N1.2014.P35-45
E. Maftu’ah, A. Maas, B. Purwanto
Peat soil is characterized by poor nitrogen (N) availability. Ameliorants are expected to rectify this problem. This research aimed to study the effect of ameliorant on N availability and N uptake by sweet corn plant in degraded peatland. The experiment was conducted in the greenhouse in May-July 2011 and on peatland of Kalampangan Village, Palangkaraya, Central Kalimantan in September-December 2011. Burnt peat soil of Kalampangan was used in the greenhouse experiment and sweet corn was used as an indicator plant. The treatments consisted of two factors, i.e. compositions of ameliorants by weight (A1 = 80% chicken manure + 20% dolomite; A2 = 80% local farm weed + 20% dolomite; A3 = 80% mineral soil + 20% dolomite; A4 = 20% chicken manure + 20% local farm weed + 20% residue of Chinese water chestnut (Eleocharis dulcis) + 20% mineral soil + 20% dolomite; and A5 = 19% chicken manure + 71.5% mineral soil + 9.5% dolomite) and rates of those ameliorants (5, 10, 15, 20 and 25 t ha-1). The experiment was arranged in a completely randomized block design with three replications. Data were collected every two weeks for five times. Observations were made on soil pH, available N (NH4+, NO3-), plant height, and N uptake in root and shoot. The results showed that treatment A1 increased soil pH and availability of NH4+ and NO3- in peat soils at the maximum vegetative stage. Treatment A1 provided the highest N availability and N uptake by the plant. Field experiment showed that N uptake increased with the plant yield. Optimum yield of fresh corn cob was obtained from treatment A1 at the rate of 20 t ha-1. This research reconfirms the effectiveness of chicken manure and dolomite as peat soil ameliorant.
{"title":"EFFECTS OF AMELIORANT COMPOSITIONS ON NITROGEN MINERALIZATION AND UPTAKE BY SWEET CORN IN DEGRADED PEATLAND","authors":"E. Maftu’ah, A. Maas, B. Purwanto","doi":"10.21082/IJAS.V15N1.2014.P35-45","DOIUrl":"https://doi.org/10.21082/IJAS.V15N1.2014.P35-45","url":null,"abstract":"Peat soil is characterized by poor nitrogen (N) availability. Ameliorants are expected to rectify this problem. This research aimed to study the effect of ameliorant on N availability and N uptake by sweet corn plant in degraded peatland. The experiment was conducted in the greenhouse in May-July 2011 and on peatland of Kalampangan Village, Palangkaraya, Central Kalimantan in September-December 2011. Burnt peat soil of Kalampangan was used in the greenhouse experiment and sweet corn was used as an indicator plant. The treatments consisted of two factors, i.e. compositions of ameliorants by weight (A1 = 80% chicken manure + 20% dolomite; A2 = 80% local farm weed + 20% dolomite; A3 = 80% mineral soil + 20% dolomite; A4 = 20% chicken manure + 20% local farm weed + 20% residue of Chinese water chestnut (Eleocharis dulcis) + 20% mineral soil + 20% dolomite; and A5 = 19% chicken manure + 71.5% mineral soil + 9.5% dolomite) and rates of those ameliorants (5, 10, 15, 20 and 25 t ha-1). The experiment was arranged in a completely randomized block design with three replications. Data were collected every two weeks for five times. Observations were made on soil pH, available N (NH4+, NO3-), plant height, and N uptake in root and shoot. The results showed that treatment A1 increased soil pH and availability of NH4+ and NO3- in peat soils at the maximum vegetative stage. Treatment A1 provided the highest N availability and N uptake by the plant. Field experiment showed that N uptake increased with the plant yield. Optimum yield of fresh corn cob was obtained from treatment A1 at the rate of 20 t ha-1. This research reconfirms the effectiveness of chicken manure and dolomite as peat soil ameliorant.","PeriodicalId":13456,"journal":{"name":"Indonesian Journal of Agricultural Science","volume":"15 1","pages":"35-45"},"PeriodicalIF":0.0,"publicationDate":"2014-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67800737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-10-16DOI: 10.21082/ijas.v14n2.2013.p63-70
B. Tangendjaja
Dried distillers grains with solubles (DDGS) is a co-product of ethanol industry made from grains through fermentation process and it can be used for animal feeding especially for dairy cows. A feeding trial on US corn DDGS was conducted at commercial dairy farm in Vietnam during hot condition in 2010. One hundred and fifty six dairy cows in a later stage of milk production were allotted randomly in three groups of 52 cows with similar milk production. Three dietary treatments offered comprised control diet, diet with 7.5% DDGS and diet with 15% DDGS. The diets were formulated to contain similar nutrient profiles and comprised forages (corn, elephant grass and alfalfa hay), brewery waste, soybean curd waste, corn, soybean meal, molasses and commercial dairy supplement. The diet was manufactured locally in total mixed ration system and delivered two times per day. Milk production, feed consump-tion and milk quality were measured for 5 days before the trial and 45 days after the trial. The result demonstrated that feeding DDGS could support higher milk production without affecting feed consumption. Feeding DDGS at 7.5% and 15% resulted in higher milk production of 2 and 4 kg per day respectively compared to cows fed control diet. Feed intake remained unaffected at around 35 kg per day. Milk quality as measured by total solid and fat content was similar in cows fed the control diet and DDGS at 7.5%. Feeding DDGS at 15% tended to have slightly better total solid and fat content. Feeding DDGS was able to reduce cost of the diets; diet cost for control, DDGS 7.5% and DDGS 15% were VND 2537, 2460 and 2399 per kg, respectively. It is concluded that DDGS can be economically used for feeding dairy cows in hot climate condition and improving milk production.
{"title":"EFFECT OF FEEDING CORN DRIED DISTILLERS GRAINS WITH SOLUBLES (DDGS) ON MILK PRODUCTION OF COW UNDER HOT CLIMATE CONDITION","authors":"B. Tangendjaja","doi":"10.21082/ijas.v14n2.2013.p63-70","DOIUrl":"https://doi.org/10.21082/ijas.v14n2.2013.p63-70","url":null,"abstract":"Dried distillers grains with solubles (DDGS) is a co-product of ethanol industry made from grains through fermentation process and it can be used for animal feeding especially for dairy cows. A feeding trial on US corn DDGS was conducted at commercial dairy farm in Vietnam during hot condition in 2010. One hundred and fifty six dairy cows in a later stage of milk production were allotted randomly in three groups of 52 cows with similar milk production. Three dietary treatments offered comprised control diet, diet with 7.5% DDGS and diet with 15% DDGS. The diets were formulated to contain similar nutrient profiles and comprised forages (corn, elephant grass and alfalfa hay), brewery waste, soybean curd waste, corn, soybean meal, molasses and commercial dairy supplement. The diet was manufactured locally in total mixed ration system and delivered two times per day. Milk production, feed consump-tion and milk quality were measured for 5 days before the trial and 45 days after the trial. The result demonstrated that feeding DDGS could support higher milk production without affecting feed consumption. Feeding DDGS at 7.5% and 15% resulted in higher milk production of 2 and 4 kg per day respectively compared to cows fed control diet. Feed intake remained unaffected at around 35 kg per day. Milk quality as measured by total solid and fat content was similar in cows fed the control diet and DDGS at 7.5%. Feeding DDGS at 15% tended to have slightly better total solid and fat content. Feeding DDGS was able to reduce cost of the diets; diet cost for control, DDGS 7.5% and DDGS 15% were VND 2537, 2460 and 2399 per kg, respectively. It is concluded that DDGS can be economically used for feeding dairy cows in hot climate condition and improving milk production.","PeriodicalId":13456,"journal":{"name":"Indonesian Journal of Agricultural Science","volume":"14 1","pages":"63-70"},"PeriodicalIF":0.0,"publicationDate":"2013-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67799784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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