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STABILITY OF ANTHOCYANIN DURING PROCESSING, STORAGE AND SIMULATED DIGESTION OF PURPLE SWEET POTATO PASTA 紫甘薯意面加工、贮藏和模拟消化过程中花青素的稳定性
Q4 Agricultural and Biological Sciences Pub Date : 2018-06-01 DOI: 10.21082/ijas.v19n1.2018.p1-8
I. Mulyawanti, S. Budijanto, S. Yasni
Purple sweet potato is rich in anthocyanin giving a potential application in food product development.  However, anthocyanin is relatively unstable and easily degraded during processing and storage. Understanding the stability and bio-accessibility of anthocyanin during processing, storage and simulated digestion is very important. The study aimed to investigate changes in anthocyanin degradation during processing, storage and simulated digestion of purple sweet potato pasta. The pasta was prepared through several processing steps, i.e. steaming the tuber, steaming the dough formula, extrusion, drying and boiling. Anthocyanin was analyzed at every stages of processing and storage of the pasta. The durability of the pasta during storage was analysed using an accelerated shelf-life testing method at 30, 40 and 50ºC for 28 days. The study showed that anthocyanin content decreased during the whole stages of processing and storage, but slightly increased during steaming. The highest loss of the anthocyanin occurred in the boiling process. Based on resistance to stomach and intestinal conditions, the bio-accessibility of anthocyanin was better in the digestive system in the stomach than that in the intestines. The increased anthocyanin appeared again in the colon. This study provides useful information for designing an effective method to minimize an extensive loss of anthocyanin of purple sweet potato for food product development.
紫甘薯富含花青素,在食品开发中具有潜在的应用前景。然而,花青素相对不稳定,在加工和储存过程中容易降解。了解花青素在加工、储存和模拟消化过程中的稳定性和生物可及性非常重要。本研究旨在研究紫甘薯意大利面在加工、储存和模拟消化过程中花青素降解的变化。意大利面是通过几个加工步骤制备的,即蒸块茎、蒸面团配方、挤压、干燥和煮沸。在意大利面加工和储存的各个阶段对花青素进行了分析。使用加速保质期测试方法在30、40和50ºC下储存28天,分析意大利面在储存过程中的耐用性。研究表明,花青素含量在整个加工和贮藏过程中都有所下降,但在蒸煮过程中略有增加。花青素损失最大的发生在煮沸过程中。基于对胃和肠道条件的抵抗力,花青素在胃的消化系统中的生物可及性比在肠道中更好。增加的花青素再次出现在结肠中。本研究为设计一种有效的方法来最大限度地减少紫甘薯花青素在食品开发中的广泛损失提供了有用的信息。
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引用次数: 12
SEED AND POLLEN TTRANSMISSION OF A NEW UNIDENTIFIED MOTTLE DISORDER OF MAIZE IN INDONESIA 印度尼西亚一种新的玉米斑点病的种子和花粉转运
Q4 Agricultural and Biological Sciences Pub Date : 2018-01-31 DOI: 10.21082/IJAS.V1N2.2000.P63-67
W. Wakman, D. Teakle, R. Louie
A new unidentified mottle disorder of maize Indonesia was found at the Research Institute for Maize and Other Cereals (RIMOC), Maros, South Sulawesi in 1995. Attempts to identify the disorder were made by mechanical inoculation, insect vector (Rhopalosiphum maidis and Peregrinus maidis) transmission, seed and pollen transmission, electron microscopy, and serological test. Fifty seeds from each of 22 ears of Arjuna maize plants showing the disorder were planted and symptoms on the seedlings were recorder at 1, 2, and 3 weeks after planting. The percentage of seedlings showing the disorder ranged from 40 to 100. Pollen of affected Arjuna was then used to pollinate four sweet corn female flowers. Hybrid seeds (50 per ear) of the crosses were planted and symptoms were recorded at 1, 2, and 3 weeks after planting. The results showed that percentage of seedlings showing the disorder ranged from 22 to 84. Electron microscopy and ELISA tests on 15 viruses and one phytospiroplasma antiserum however, gave negative results. Therefore, maize disorder at Maros was not identical to any known viral disease of maize. It could be a genetical disorder and has been given the name maize mottle.
1995年,在南苏拉威西省马罗斯市的玉米和其他谷物研究所(RIMOC)发现了一种新的印度尼西亚玉米斑点病。试图通过机械接种、昆虫载体(Rhopalosiphum maidis和Peregrinus maidis)传播、种子和花粉传播、电子显微镜和血清学测试来鉴定这种疾病。从表现出该病症的22穗阿诸那玉米植株中的每穗种植50颗种子,并在种植后1、2和3周记录幼苗上的症状。表现出这种紊乱的幼苗的百分比在40到100之间。受影响的阿诸那的花粉被用来为四朵甜玉米雌花授粉。种植杂交种(每穗50粒),并在种植后1、2和3周记录症状。结果表明,表现出该病症的幼苗的百分比在22到84之间。然而,对15种病毒和一种植物螺旋体抗血清的电子显微镜和ELISA测试结果均为阴性。因此,Maros的玉米疾病与任何已知的玉米病毒性疾病都不相同。它可能是一种遗传性疾病,被命名为玉米斑点病。
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引用次数: 0
PERFORMANCE OF POTATOES GROWN FROM TRUE SEED UNDER DIFFERENT ENVIRONMENTAL CONDITIONS IN WEST JAVA 爪哇西部不同环境条件下马铃薯的生长性能
Q4 Agricultural and Biological Sciences Pub Date : 2018-01-31 DOI: 10.21082/IJAS.V1N2.2000.P29-38
N. Gunadi, P. Harris
True potato seed (TPS) has been considered as an alternative planting material of seed tuber in potato production, especially in developing countries where good quality of seed tuber at reasonable price is difficult to obtain. This study was conducted to investigate the effects of environment and genotype on potatoes grown from TPS in contrasting seasons and sites in West Java, Indonesia over a period of two years (September 1990-October 1992). Different method of establishing the potato crop from TPS were tested and compared with  ev. Granola established from seed tubers. Data were interpreted in terms of relationships between total and tuber dry matter yield and intercepted radiation (IR). The growth and yield of potatoes grown from TPS were generally better at higher rather than lower altitudes, probably related to the lower temperatures which was reflected in an increase in IR and in the efficiency of conversion of IR into total and tuber dry matter. In the highland, seedling transplants and seedling tubers were both suitable for establishing crops from TPS in the wet season, but not in the dry season. All progenies, in general performed well at the highland especially the medium-late maturing progenies such as HPS 7/13. Atlantic x LT-7 initiated tubers and matured more rapidly than the other progenies and performed well at the lower altitudes. Atzimba x DTO-28 had a more gradual decline in ground cover and was more resistant to late blight than other early-medium maturing progenies. The medium-late maturing progeny. Atzimba x R-128.6 matured later and performed poorly at the lower altitudes.
马铃薯真籽(TPS)一直被认为是马铃薯生产中种薯的替代种植材料,尤其是在发展中国家,在这些国家很难以合理的价格获得优质的种薯。本研究旨在调查环境和基因型对印度尼西亚西爪哇不同季节和地点(1990年9月至1992年10月)种植的TPS土豆的影响。试验了从TPS中建立马铃薯作物的不同方法,并与从种子块茎中建立Granola进行了比较。根据总干物质产量和块茎干物质产量与截获辐射(IR)之间的关系对数据进行了解释。用TPS种植的土豆在海拔较高而不是较低的地方生长和产量通常更好,这可能与较低的温度有关,这反映在IR的增加以及IR转化为总干物质和块茎干物质的效率上。在高原,幼苗移植和幼苗块茎都适合在雨季从TPS建立作物,但在旱季不适合。一般来说,所有后代在高地表现良好,尤其是中晚熟后代,如HPS 7/13。Atlantic x LT-7启动块茎,比其他后代成熟得更快,在低海拔地区表现良好。Atzimba x DTO-28的地被覆盖物逐渐减少,与其他中早熟后代相比,对晚疫病的抗性更强。中晚熟的后代。Atzimba x R-128.6成熟较晚,在较低海拔地区表现不佳。
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引用次数: 0
SOIL OF PAMETIKARATA, EAST SUMBA: ITS SUITABILITY AND CONSTRAINTS FOR FOOD CROP DEVELOPMENT 东松巴帕梅提卡拉塔土壤:对粮食作物发展的适宜性和制约因素
Q4 Agricultural and Biological Sciences Pub Date : 2018-01-31 DOI: 10.21082/IJAS.V1N1.2000.P1-9
B. Prasetyo, H. Sosiawan, S. Ritung
Pametikarata, Lewa subdistrict, is the priority area for food crop development in East Sumba. To evaluate its suitability and constraints, chemical properties, suitability and fertility capability classification for rice and secondary crops of some potential soils have been studied both in the field and in the laboratory. Seven soil profiles consisted of forty one soil samples were subjected to chemical and mineralogical analyses in the laboratory. The analyses consisted of clay fraction and organic-carbon contents, pH, potential P and K (25% HCI extraction), available P, phosphate retention. Exchangeable cations and cation exchange capacity (NH4OAc 1N, pH 7), and mineralogical composition of the clay fraction. The results indicate that soil acidity varies from acid to neutral Exchangeable cations are dominated by Ca cation and soil CEC ranges from low to very high. Clay mineral composition also varies, some are dominated by montmorillonite, others show mixed mineralogy between montmorillonite and kaolinite, and the rests are dominated by kaolinite with the exception of wet Vertisols, all soils are grouped as marginally suitable (S3) for rice and secondary crops. Nutrient availability and retention are the common limiting factors. Using fertility capability classification (FCC), all soils are grouped as clayey soils with low infiltration and high water holding capacity. The serious constraint for food crop development in this area is uncontrolled grazing that makes a conflict of interests between farming and cattle herding systems. Optimum success of food crop development in the area could be reached by controlling the herding system and improving the existing agricultural system.
勒瓦区Pametikarata是东萨姆巴粮食作物开发的优先地区。为了评价其适宜性和限制条件,在田间和实验室对一些潜在土壤的化学性质、对水稻和次生作物的适宜性和肥力分类进行了研究。由四十一个土壤样本组成的七个土壤剖面在实验室中进行了化学和矿物学分析。分析包括粘土组分和有机碳含量、pH、电位P和K(25%HCI提取)、有效P和磷酸盐保留率。可交换阳离子和阳离子交换容量(NH4OAc 1N,pH 7),以及粘土部分的矿物学组成。结果表明,土壤酸度从酸性到中性不等。可交换阳离子以Ca阳离子为主,土壤CEC从低到极高。粘土矿物成分也各不相同,一些以蒙脱石为主,另一些表现出蒙脱石和高岭石之间的混合矿物学,其余的以高岭石为主,除了湿的Vertisol,所有土壤都被归类为勉强适合水稻和次生作物的土壤(S3)。养分的可利用性和滞留性是常见的限制因素。使用肥力能力分类(FCC),所有土壤都被归类为低渗透和高持水能力的粘性土。该地区粮食作物发展的严重制约因素是不受控制的放牧,这导致农业和畜牧系统之间的利益冲突。该地区粮食作物发展的最佳成功可以通过控制放牧系统和改善现有农业系统来实现。
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引用次数: 1
QUALITY CHANGES OF SARDINES (Sardinella neopilchardus) AT INDONESIAN AMBIENT TEMPERATURE 印尼环境温度下沙丁鱼(沙丁鱼)的品质变化
Q4 Agricultural and Biological Sciences Pub Date : 2018-01-31 DOI: 10.21082/IJAS.V1N1.2000.P21-28
F. Ariyani
In tropical countries such as Indonesia, high ambient temperature and a lack of icing cause very rapid spoilage of fish. Leaving fish uniced while waiting for processing is a common practice resulting in lower quality of the end products, and consequently lower price of the products. To study the spoilage pattern of sardines ( Sardinella neophilchardus ) in a tropical region, and to investigate a simple and quick method to judge the degree of spoilage, observations on the deterioration of sardines were carried out by incubating fish in a humidity chamber adjusted to 28-30 o C. RH 70-80% for up to 14 h. The changes of sensory attributes based on demerit point score. pH, and total volatile base nitrogen (TVB-N) were monitored to determine the degree of spoilage. Although the appearance of sardines was still bright after 6-h incubation time, fish were not as fresh as those without incubation and the belly and tissue were softened. After incubation time of 10 h, fish showed slight to moderate signs of spoilage with demerit point score of 25 out of the maximum score of 39, and a TVB-N content of 20 mg%N. This is similar to the fish quality commonly used in the commercial production of dried-salted fish in Indonesia. Fish incubated for 12 h or more appeared completely spoiled and were rejected by sensory panel. A high correlation between demerit point score and TVB-N was noted, therefore the demerit point system which was more suitable, quicker, and easier could be used to determine the degree of fish spoilage.
在印度尼西亚等热带国家,高环境温度和缺乏结冰导致鱼类迅速变质。在等待加工时将鱼分开是一种常见的做法,导致最终产品的质量降低,从而降低产品的价格。为研究热带地区沙丁鱼(Sardinella neophilchardus)的腐败规律,探索一种简单快速的腐败程度判断方法,将沙丁鱼放在28-30℃的湿度箱中,RH 70-80%,孵育14 h,观察其变质情况。通过监测pH和总挥发性碱氮(TVB-N)来确定腐败程度。孵育6小时后,虽然沙丁鱼的外观仍然明亮,但鱼的新鲜度不如未孵育的鱼,腹部和组织都软化了。孵育10小时后,鱼表现出轻微到中度的腐败迹象,记过分为25分(满分为39分),TVB-N含量为20 mg%N。这与印度尼西亚商业生产中通常使用的咸鱼干的鱼质量相似。孵育12小时或更长时间的鱼完全变质,被感觉面板拒绝。记过分与TVB-N之间有较高的相关性,因此记过分制度更适合、更快捷、更容易确定鱼类的腐败程度。
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引用次数: 1
IDENTIFICATION AND PATHOGENICITY OF ISOLATE OF BACTERIUM CAUSED LEAF BLIGHT DISEASE ON Maranta arundinacea 马兰叶枯病细菌分离株的鉴定及致病性研究
Q4 Agricultural and Biological Sciences Pub Date : 2018-01-31 DOI: 10.21082/IJAS.V1N1.2000.P10-15
S. Supriadi, N. Ibrahim, N. Karyani
Arrowroot ( Maranta arundinacea L ) is a multi-functional plant used as a source of medicinal, carbohydrate (especially the green leaf type) and also as ornamental plant (the streaked white leaf type). A leaf blight disease is recently found on the streaked white type in Bogor. Preliminary observation indicated that the disease was associated with bacterial infection. The cause of the disease has not been studied. This study was aimed to identify the cause of bacterial leaf blight disease. Experiments were conducted in the laboratory of Research Institute for Spice and Medicinal Crops in Bogor. Suspected bacteria were isolated from diseased leaves. The results showed that the bacterium produced white to brownish colonies on rich agar media containing peptone or cassamino acid. 3-5 mm in diameter, circular, and did not yield fluorescent pigment on King’s B medium. The bacterium formed rod cells, Gram negative, accumulated poly β hydroxybutyrate, utilized glucose under aerobic condition, not hydrolyse arginine and starch, positive catalase, insensitive to tetrazolium salt (0.1%), and grew at 35 o C. The bacterium neither producted xanthomonadin pigment nor reduced nitrate to nitrite. The pathogen was tolerant to penicillin and oxolinic acid, but sensitive to streptomycin and oxytetracycline at high concentration (1.000 ppm). These characteristics met to those of Pseudomonas cepacia . Pathogenicity test on detached leaves showed that the typical symptom of blight was similar to that of natural infection on arrowroot. This is the first report on occurrence of P cepacia on arrowroot plant.
竹芋(Maranta arundinacea L)是一种多功能植物,具有药用、碳水化合物(尤其是绿叶型)和观赏植物(条纹白叶型)的双重用途。最近在茂物发现了一种叶枯病。初步观察表明该病与细菌感染有关。这种疾病的病因尚未得到研究。本研究旨在查明细菌性叶枯病的病因。实验在茂物香料和药用作物研究所实验室进行。从病叶中分离出疑似细菌。结果表明,细菌在富含蛋白胨或木薯氨基酸的琼脂培养基上产生白色至棕色菌落。直径3-5 mm,圆形,在King’s B培养基上不产生荧光色素。细菌形成杆状细胞,革兰氏阴性,积累聚β羟基丁酸,在好氧条件下利用葡萄糖,不水解精氨酸和淀粉,过氧化氢酶阳性,对四氮唑盐不敏感(0.1%),在35℃下生长。细菌既不产生黄花青素色素,也不将硝酸盐还原为亚硝酸盐。病原菌对青霉素和草酸耐受,但对高浓度(1.000 ppm)的链霉素和土霉素敏感。这些特征符合洋葱假单胞菌的特征。离体叶片的致病性试验表明,叶枯病的典型症状与自然侵染的症状相似。本文首次报道了绿豆病在竹属植物上的发生。
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引用次数: 0
Effect of Aluminum on Plant Growth, Phosporus and Calcium Uptake of Tropical Rice (Oryza Sativa), Maize (Zea Mays), and Soybean (Glycine Max) 铝对热带水稻(Oryza Sativa)、玉米(Zea Mays)和大豆(Glycine Max)植株生长、磷和钙吸收的影响
Q4 Agricultural and Biological Sciences Pub Date : 2018-01-31 DOI: 10.21082/IJAS.V1N2.2000.P51-62
D. Nursyamsi, M. Osaki, T. Tadano
Aluminum toxicity is the most limiting factor to plant growth on acid soils. Structural and functional damages in the root system by Al decrease nutrient uptake and lead to reduce plant growth and mineral deficiency in shoot. Greenhouse experiment was conducted to study the effect of Al on plant growth, and P and Ca uptake of rice, maize, and soybean. The plants were grown in hydroponic solution added with 0, 5, 10, and 30 ppm Al, at pH 4.0. The results showed that relative growth of shoots and roots of upland rice, lowland rice, maize, and soybean decreased with an increase of Al level. However, sometimes the low Al level (5 ppm) stimulated shoot and root growth of some varieties in these species. According to total AlRG30 values, which is Al concentration in solution when relative growth decreased to 50%, Al tolerance of species was in order of barley < maize < soybean < lowland rice < upland rice. For maize, Al tolerance was in the order of Arjuna < Kalingga < P 3540 < SA 5 < SA 4 < PM 95 A < SA 3 < Antasena; for soybean was Wilis < INPS < Galunggung < Kerinci < Kitamusume; for lowland rice was RD 23 < Kapuas < Cisadane < KDML 105 < IR 66 < RD 13, and for upland rice was Dodokan < JAC165 < Cirata < Orizyca sabana 6 < Danau Tempe < Laut Tawar. Based on the rank of Al tolerance, rice was the useful crop to be planted in acid soils. Antasena (maize), Kitamusume ( soybean ), RD 13 (lowland rice), and Laut Tawar (upland rice) were also recommended for acid soils. P and Ca concentration in shoots and roots commonly decreased with an increase of Al level. However, the low Al level stimulated absorption of P and Ca concentrations in shoots and roots.
铝毒性是酸性土壤上植物生长的最大限制因素。铝对根系结构和功能的损害降低了植物对养分的吸收,导致植株生长减缓和地上部矿物质缺乏。通过温室试验研究了铝对水稻、玉米和大豆植株生长及磷、钙吸收的影响。在pH为4.0的水培溶液中分别添加0、5、10和30 ppm的铝。结果表明,旱稻、旱稻、玉米和大豆的茎和根的相对生长量随铝水平的升高而降低。然而,有时低铝水平(5 ppm)刺激了这些物种的一些品种的茎和根的生长。根据AlRG30总值(即相对生长量降低至50%时溶液中的Al浓度),各品种对Al的耐受性依次为大麦<玉米<大豆<低地水稻<旱稻。玉米对铝的耐受性依次为Arjuna < Kalingga < P 3540 < sa5 < sa4 < PM 95 A < sa3 < Antasena;大豆品种为Wilis < INPS < Galunggung < Kerinci < kitamusme;低地水稻为RD 23 < Kapuas < Cisadane < KDML 105 < IR 66 < RD 13,旱地水稻为Dodokan < JAC165 < Cirata < Orizyca sabana 6 < Danau Tempe < Laut Tawar。从耐铝性的排序来看,水稻是酸性土壤适宜种植的作物。玉米Antasena、大豆kitamusme、低地水稻RD 13和旱地水稻Laut Tawar也被推荐用于酸性土壤。根系和茎部磷、钙浓度普遍随铝水平的升高而降低。然而,低铝水平刺激了茎和根对磷和钙的吸收。
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引用次数: 1
EMPOWERING WOMEN FARMERS THROUGHT MULTICHANNEL LEARNING: AN APPLICATION OF NEED ASSESSMENT TECHNIQUE 通过多渠道学习增强女农民能力&需求评估技术的应用
Q4 Agricultural and Biological Sciences Pub Date : 2018-01-31 DOI: 10.21082/IJAS.V1N2.2000.P39-50
Fawzia Sulaiman
The suitability and effectiveness of material, method, and media used in nonformal education provided by government and nongovernment institutions (including agricultural extension) has been questioned in recent years, this is especially true for nonformal education catering for poor women farmers. Even though the significant roles and contribution of woman farmers in farming and family economic survival in Indonesia have been widely known, their nonformal educations need are rarely acknowledged. Several socio-cultural norms and values hinder women farmers’ participation in extension activities. The perception that agricultural extension activities fall under public domain has resulted that these activities are under male control (usually head for household). Without special intervention and accurate need assessment for nonformal education, the gap between women farmers and their male counterparts will be wider. This study was designed to identify nonformal educational needs of poor women farmers in Sakra Subdistrict, East Lombok, West Nusa Tenggara. The primary data and information were solicited through the Participatory Rural Appraisal (PRA) method using focus group interviews and gender analysis techniques. The result indicated that women farmers in the study area could not afford to participate in learning activities, unless the learning program offer a direct benefit to generate income. However, in general they put a high expectation in their children’s education and learning opportunity that would lead them to earn a better income. Due to their very limited educational background, demonstration and learning by doing method where perceived to be the most effective extension methods. Furthermore, radio and television were not effective educational channels, since there were only a very small number of respondents who owned radio and TV sets. In the efforts to empower women farmers, field extension workers require training on socio-economics, including gender analysis, and need assessment methodology to better perform their tasks in the field.
近年来,政府和非政府机构提供的非正规教育(包括农业推广)所使用的材料、方法和媒介的适宜性和有效性受到质疑,尤其是针对贫困女农民的非正规教育。尽管妇女农民在印度尼西亚农业和家庭经济生存方面的重要作用和贡献已广为人知,但她们的非正规教育需求却很少得到承认。一些社会文化规范和价值观阻碍了女农民参与推广活动。认为农业推广活动属于公共领域的观念导致这些活动由男性(通常是户主)控制。如果没有对非正规教育的特别干预和准确的需求评估,女性农民与男性农民之间的差距将会扩大。本研究旨在确定东龙目岛、西努沙登加拉岛Sakra街道贫困女农民的非正规教育需求。主要数据和信息是通过参与式农村评估(PRA)方法,利用焦点小组访谈和性别分析技术收集的。结果表明,研究地区的女农民没有能力参加学习活动,除非学习计划能提供直接的收益来创造收入。然而,总的来说,他们对孩子的教育和学习机会寄予很高的期望,这将使他们获得更好的收入。由于他们的教育背景非常有限,示范和边做边学被认为是最有效的扩展方法。此外,广播和电视不是有效的教育渠道,因为只有极少数答复者拥有收音机和电视机。在赋予妇女农民权力的努力中,实地推广工作人员需要接受社会经济学方面的培训,包括性别分析,并需要评估方法,以便更好地在实地执行任务。
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引用次数: 0
PRELIMINARY ECOLOGOCAL STUDY OF Rhizobium : SELECTION OF ANTIBIOTIC RESISTANT MUTANTS OF Rhizobium PMA295 NODULATING Sesbania sesban 根瘤菌的初步生态学研究:田菁根瘤菌PMA295耐药突变体的筛选
Q4 Agricultural and Biological Sciences Pub Date : 2018-01-31 DOI: 10.21082/IJAS.V1N1.2000.P16-20
N. Purwantari
This study was designed to provide strain for studying the ecology of Rhizobium when it was used to inoculate seed sown in the soil or in the field. Naturally occurring antibiotic resistant mutant strains of Rhizobium PMA295 nodulating Sesbania sesban were selected by antibiotic selection method. The isolates should be as effective  in nitrogen fixation as the parental  wild-type strain. Authentication of mutant isolates showed that these rifampicin mutant isolates could nodulate test plant and most nodules formed by the rifampicin 50 ppm and streptomycin 500 ppm mutant isolates were red in colour. These twenty isolates can be regarded as rhizobia. The most effective mutant, isolates PMA295 selected on rifampicin 50 ppm was also resistant to rifampicin 60, 70, 80, and 90 ppm. The effectiveness of most mutant strains was not significantly different from their parent strains. The rifampicin 50 ppm mutant strain of Rhizobium PMA295 could be used as inoculant for ecological study.
本研究旨在为根瘤菌在土壤或田间接种种子时的生态学研究提供菌株。采用抗生素筛选法筛选天然耐药田菁根瘤菌PMA295突变株。分离株的固氮作用应与亲本野生型菌株一样有效。对突变株的鉴定表明,利福平突变株能形成根瘤,利福平50 ppm和链霉素500 ppm突变株形成的根瘤大部分呈红色。这20个分离株可视为根瘤菌。在50 ppm的利福平上选择的最有效的突变株PMA295也对60、70、80和90 ppm的利福平耐药。大多数突变菌株的效力与其亲本菌株没有显著差异。利福平50ppm突变菌株PMA295可作为接种剂进行生态学研究。
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引用次数: 0
DIVERSITY AND CAPABILITY ANALYSES OF FERTILITY RESTORER GENES OF CYTOPLASMIC MALE STERILE RICE LINES USING SSR 利用SSR分析水稻细胞质雄性不育系育性恢复基因的多样性和能力
Q4 Agricultural and Biological Sciences Pub Date : 2017-12-29 DOI: 10.21082/ijas.v18n2.2017.p43-50
Y. Widyastuti, M. Yunus, B. S. Purwoko, Satoto Satoto
Development of hybrid rice depends on the effectivity of cytoplasmic male sterility (CMS) and restorer (R) lines.  The molecular genetic approach is expected to help the breeder in identification of suitable parental lines to hybrid rice improvement. The study aimed to assess genetic relationship among three types of CMS systems (wild  abbortive/WA Kalinga and Gambiaca) as female parents and to identify diversity of genes controlling fertility restoration in rice. The study used nine F 1 hybrids and F 2 populations obtained from the hybridization of three different CMS lines (IR58025A-WA, IR80156A-Kalinga and IR80154A-Gambiaca) with three restorer lines (PK90, PK12 and BP11). Fifteen SSR markers were used to select genomic regions of chromosome 1 and 10 on which Rf3 and Rf4 genes located in the hybrids. The results showed that fertility restoration in CMS-WA and CMS-Gambiaca was governed by two independent and dominant genes ( Rf3 and Rf4 ), while in CMS-Kalinga the fertility restoration was controlled by one single dominant gene. Biological processes occurred in the fertility restoration of the hybrids were the same based on the pollen and spikelet fertilities of F 1 hybrids derived from three CMS and R lines, i.e. 76.1–78.3% and 69.1–76.6%, respectively. A restorer line PK12 had a higher capability in fertility restoration than PK90 and BP11. The SSR primers RM490 and RM258 were capable of identifying the Rf3 and Rf4 genes controlled fertility restoration in CMS-WA. The study supports the use of male sterile WA in rice hybridization.
杂交水稻的发育取决于细胞质雄性不育系和恢复系的有效性。分子遗传学方法有望帮助育种人员确定适合杂交水稻改良的亲本系。本研究旨在评估三种类型的CMS系统(野生abportive/WA Kalinga和Gambiaca)作为雌性亲本之间的遗传关系,并确定控制水稻生育能力恢复的基因多样性。本研究采用三个不同不育系(IR58025A-WA、IR80156A-Kalinga和IR80154A-Gambiaca)与三个恢复系(PK90、PK12和BP11)杂交获得的9个F1杂交种和F2群体。利用15个SSR标记选择杂交种中Rf3和Rf4基因所在的1号和10号染色体的基因组区域。结果表明,CMS-WA和CMS Gambiaca的育性恢复由两个独立的显性基因(Rf3和Rf4)控制,而CMS Kalinga的育力恢复由一个单一的显性基因控制。基于三个不育系和R系的F1杂种的花粉和小穗受精率,杂种在育性恢复中发生的生物学过程是相同的,分别为76.1–78.3%和69.1–76.6%。恢复系PK12的育性恢复能力高于PK90和BP11。SSR引物RM490和RM258能够鉴定控制CMS-WA生育恢复的Rf3和Rf4基因。该研究支持在水稻杂交中使用雄性不育WA。
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引用次数: 2
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Indonesian Journal of Agricultural Science
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