Antioxidant compounds are necessary to block the initiation of oxidation chain reactions and inhibit the formation of ROS which are the cause of various diseases. Natural antioxidants can be isolated from various sources, one of them from Curcuma aeruginosa Roxb Rhizome. This study was aimed to isolate and identify antioxidant compounds from Curcuma aeruginosa Roxb rhizome. To isolate the active compound, the 2,2-diphenyl-1-picrylhidrazyl (DPPH) free radical scavenging activity guide was used. Initially the Curcuma aeruginosa Roxb rhizome powdered material macerated with ethanol 70% to give ethanol extract (EE). The EE was then fractionated gradually with increasing polarity solvents to give n-hexane (HF), ethyl acetate (EAF), ethanol (EF) and insoluble (IF) fractions. The EF was the active fraction, was fractionated into 6 fractions (EE.a-f) based on their TLC picture. Two compounds (EF.a1 and EF.a2) were isolated from active fraction (EF.a) by preparative TLC. The EF.a2 appeared as white crystals demonstrates better activity as anti radical DPPH, having 96.24% purity (HPLC). EF.a2 appeared as a single peak in GC (Rt 12,78), and having molecular weight at m/z 234. There were 2 absorbtion peaks at 226 nm and 260 nm in Uv-Vis spectrophotometer and absorption bands at 3364, 3314, 2905 and 1653 cm-1 on the FT-IR spectrum. Based on spectroscopic data (1H-,13C-NMR) and comparison with reported data, the EF.a2 was identified as sesquiterpene, curcumenotone (C15H22O2). The curcumenotone was shown potent antioxidant in DPPH radical scavenging assay had an IC50 53.24±1.51 µg/mL, ABTS radical scavenging assay had an IC50 41.33±3.15 µg/mL, and FRAP assay had an IC50 37,82±2.02 µg/mL.
{"title":"Isolation and Characterization of Curcumenotone, a Sesquiterpene from Curcuma aeruginosa Roxb as Antioxidant","authors":"Ririn Suharsanti, Subagus Wahyuono, Puji Astuti, Nunung Yuniarti","doi":"10.22146/ijp.7799","DOIUrl":"https://doi.org/10.22146/ijp.7799","url":null,"abstract":"Antioxidant compounds are necessary to block the initiation of oxidation chain reactions and inhibit the formation of ROS which are the cause of various diseases. Natural antioxidants can be isolated from various sources, one of them from Curcuma aeruginosa Roxb Rhizome. This study was aimed to isolate and identify antioxidant compounds from Curcuma aeruginosa Roxb rhizome. To isolate the active compound, the 2,2-diphenyl-1-picrylhidrazyl (DPPH) free radical scavenging activity guide was used. Initially the Curcuma aeruginosa Roxb rhizome powdered material macerated with ethanol 70% to give ethanol extract (EE). The EE was then fractionated gradually with increasing polarity solvents to give n-hexane (HF), ethyl acetate (EAF), ethanol (EF) and insoluble (IF) fractions. The EF was the active fraction, was fractionated into 6 fractions (EE.a-f) based on their TLC picture. Two compounds (EF.a1 and EF.a2) were isolated from active fraction (EF.a) by preparative TLC. The EF.a2 appeared as white crystals demonstrates better activity as anti radical DPPH, having 96.24% purity (HPLC). EF.a2 appeared as a single peak in GC (Rt 12,78), and having molecular weight at m/z 234. There were 2 absorbtion peaks at 226 nm and 260 nm in Uv-Vis spectrophotometer and absorption bands at 3364, 3314, 2905 and 1653 cm-1 on the FT-IR spectrum. Based on spectroscopic data (1H-,13C-NMR) and comparison with reported data, the EF.a2 was identified as sesquiterpene, curcumenotone (C15H22O2). The curcumenotone was shown potent antioxidant in DPPH radical scavenging assay had an IC50 53.24±1.51 µg/mL, ABTS radical scavenging assay had an IC50 41.33±3.15 µg/mL, and FRAP assay had an IC50 37,82±2.02 µg/mL.","PeriodicalId":13520,"journal":{"name":"INDONESIAN JOURNAL OF PHARMACY","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135307240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nimodipine is a dihydropyridine calcium channel blocker that shares the general properties of nifedipine, but acts mainly on cerebral blood vessels. Nimodipine belongs to the Class II Biopharmaceutical Classification System (BCS) drug group, which has low solubility and high permeability. The purpose of this study was to look at the differences in the yield of nanoparticles between the dry grinding and wet grinding methods on the physicochemical properties, solubility, and dissolution rate of nimodipine nanoparticles. The nanoparticles were prepared a nimodipine: HPMC ratio of 1:0.6 using two different methods. Sample characterization was carried out using Particle Size Analyzer (PSA), Scanning Electron Microscopy (SEM), Differential Scanning Calorimetry (DSC), X-ray Diffraction (XRD), and Fourier Transform Infrared (FT-IR). The Solubility test was carried out in CO2-free distilled water and the dissolution rate was carried out in phosphate buffer pH 7.2. The solubility of pure nimodipine in CO2-free distilled water was 0.339 µg/mL, the physical mixture was 1.948 µg/mL, the dry grinding nanoparticles were 3.367 µg/mL, and the wet grinding nanoparticles were 19.952 µg/mL. The dissolution test results obtained with the percentage of pure nimodipine dissolution at 60th minutes was 33.947%, the physical mixture was 39.482%, the dry grinding nanoparticles were 49.798%, and the wet grinding nanoparticles were 56.484%. Based on the results of the study, it can be said that the nimodipine-HPMC nanoparticles significantly increased the solubility and dissolution rate of nimodipine.
{"title":"Effectiveness of Dry Grinding and Wet Grinding Methods on Solution and Dissolution Rate of Nimodipine-HPMC Nanoparticles","authors":"Muthia Fadhila","doi":"10.22146/ijp.7267","DOIUrl":"https://doi.org/10.22146/ijp.7267","url":null,"abstract":"Nimodipine is a dihydropyridine calcium channel blocker that shares the general properties of nifedipine, but acts mainly on cerebral blood vessels. Nimodipine belongs to the Class II Biopharmaceutical Classification System (BCS) drug group, which has low solubility and high permeability. The purpose of this study was to look at the differences in the yield of nanoparticles between the dry grinding and wet grinding methods on the physicochemical properties, solubility, and dissolution rate of nimodipine nanoparticles. The nanoparticles were prepared a nimodipine: HPMC ratio of 1:0.6 using two different methods. Sample characterization was carried out using Particle Size Analyzer (PSA), Scanning Electron Microscopy (SEM), Differential Scanning Calorimetry (DSC), X-ray Diffraction (XRD), and Fourier Transform Infrared (FT-IR). The Solubility test was carried out in CO2-free distilled water and the dissolution rate was carried out in phosphate buffer pH 7.2. The solubility of pure nimodipine in CO2-free distilled water was 0.339 µg/mL, the physical mixture was 1.948 µg/mL, the dry grinding nanoparticles were 3.367 µg/mL, and the wet grinding nanoparticles were 19.952 µg/mL. The dissolution test results obtained with the percentage of pure nimodipine dissolution at 60th minutes was 33.947%, the physical mixture was 39.482%, the dry grinding nanoparticles were 49.798%, and the wet grinding nanoparticles were 56.484%. Based on the results of the study, it can be said that the nimodipine-HPMC nanoparticles significantly increased the solubility and dissolution rate of nimodipine.","PeriodicalId":13520,"journal":{"name":"INDONESIAN JOURNAL OF PHARMACY","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135052451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of the work was to enhance the solubilization of Olmesartan-medoxomil (OM) and formulate stable, rapidly-dissolving-tablet formulations. OM, on the other hand, is classified as Biopharmaceutics Classification System (BCS) Class II drug, which indicates that it is characterized by water-insolubility. Therefore, increasing its solubilization has the potential to boost its bioavailability. For the OM evaluation, a new HPLC technique was invented and then validated in accordance with international standards. The formulation of tablets involved co-milling with certain superdisintegrants, specifically croscarmellose sodium (Ac-Di-Sol) and crospovidone in varying percentages, followed by mixing with pH-adjusting substances such as calcium carbonate to increase solubilization in cases where the drug is soluble in alkaline. Following the evaluation of the created formulations, the optimized formulations were selected for further stability assessment. Co-milling process with crospovidone greatly improved the olmesartan release. The optimized formulations were OD11 and OD12, which exhibited fast disintegration, and the release exceeded 90% within 10 min, while the release for Olmesartan medoxomil pure standard was 9.8% after 10 min. The OD11 and OD12 were chosen for further stability assessment and revealed good stability behavior, as the study on optimized formulations revealed that the degradation was less than 5% after storage for six months at 40 °C and 75% relative humidity. Some formulations exhibited good results in terms of disintegration and release. The results of the formulations (OD11 and OD12) suggested that co-grinding with crospovidone may increase the solubilization of OM to greater than 90% after 10 minutes.
{"title":"Co-Milling: A Successful Approach to Enhance Solubility of a Poorly Soluble Antihypertensive Drug","authors":"Marwa Malik Kamil, Asmaa Abdelaziz Bayoumi","doi":"10.22146/ijp.7072","DOIUrl":"https://doi.org/10.22146/ijp.7072","url":null,"abstract":"The aim of the work was to enhance the solubilization of Olmesartan-medoxomil (OM) and formulate stable, rapidly-dissolving-tablet formulations. OM, on the other hand, is classified as Biopharmaceutics Classification System (BCS) Class II drug, which indicates that it is characterized by water-insolubility. Therefore, increasing its solubilization has the potential to boost its bioavailability. For the OM evaluation, a new HPLC technique was invented and then validated in accordance with international standards. The formulation of tablets involved co-milling with certain superdisintegrants, specifically croscarmellose sodium (Ac-Di-Sol) and crospovidone in varying percentages, followed by mixing with pH-adjusting substances such as calcium carbonate to increase solubilization in cases where the drug is soluble in alkaline. Following the evaluation of the created formulations, the optimized formulations were selected for further stability assessment. Co-milling process with crospovidone greatly improved the olmesartan release. The optimized formulations were OD11 and OD12, which exhibited fast disintegration, and the release exceeded 90% within 10 min, while the release for Olmesartan medoxomil pure standard was 9.8% after 10 min. The OD11 and OD12 were chosen for further stability assessment and revealed good stability behavior, as the study on optimized formulations revealed that the degradation was less than 5% after storage for six months at 40 °C and 75% relative humidity. Some formulations exhibited good results in terms of disintegration and release. The results of the formulations (OD11 and OD12) suggested that co-grinding with crospovidone may increase the solubilization of OM to greater than 90% after 10 minutes.","PeriodicalId":13520,"journal":{"name":"INDONESIAN JOURNAL OF PHARMACY","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135052464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Hertiani, Endra Pujiastuti, Agung Endro Nugroho, Khoirun Nisa
Diabetes mellitus (DM) is a chronic metabolic disorder characterized by increased blood glucose and abnormal carbohydrates, fats and proteins metabolism. Syzygium cumini (L.) Skeels (Myrtaceae) are widely used in anti-diabetic remedies and at the same time, rich in polyphenols. This study aims to provide a comprehensive review on the role of phytochemicals in Syzygium cumini to contribute in its anti-diabetic. The review covers related articles to antidiabetic AND Syzygium cumini AND phytochemicals OR bioactive which were published from 2012 to 2023. A systematic search was conducted by using databases Pubmed, Science Direct, Scopus, and Google The inclusion criteria include articles written in English as experimental research or clinical trials, randomized studies, and should contains phytochemical content profiling The exclusion criteria were other type of reports and or irrelevant to aforementioned topic. This review was written according to the reporting items guidelines for systematic payments and Meta-analysis (PRISMA), and SYRCLE's risk of bias (RoB) tool. By the systematic compensation used, 13 articles that meet the requirements were obtained and were further thoroughly reviewed. Seeds, leaves and bark of plant parts were reported to non-toxic in acute experiments on mice or rat. S. cumini contains flavonol glycosides, especially myricetin, myricitrin, quercetin, and kaempferol; phenolics, such as ellagic acid, tannins, gallic acid; alkaloids and saponins. These compounds contribution to the overall anti-diabetic activity were discussed, i.e increase of insulin sensitivity, secretion and usage of glucose in tissues; reducing insulin resistance, oxidative stress, gluconeogenesisand absorption of carbohydrates and sucrose. The seeds and leaves of S. cumini exhibit high potential to be developed as herbal products for anti-diabetics. This review finds a gap in research to support the development of the herbs as herbal products for anti-diabetics, in form of established preclinical and clinical trials and a reliable analytical methods for the phytochemical profiling.
{"title":"Revealing the contribution of phytochemicals in Syzygium cumini as-diabetics: A Systematic Review","authors":"T. Hertiani, Endra Pujiastuti, Agung Endro Nugroho, Khoirun Nisa","doi":"10.22146/ijp.6812","DOIUrl":"https://doi.org/10.22146/ijp.6812","url":null,"abstract":"Diabetes mellitus (DM) is a chronic metabolic disorder characterized by increased blood glucose and abnormal carbohydrates, fats and proteins metabolism. Syzygium cumini (L.) Skeels (Myrtaceae) are widely used in anti-diabetic remedies and at the same time, rich in polyphenols. This study aims to provide a comprehensive review on the role of phytochemicals in Syzygium cumini to contribute in its anti-diabetic. The review covers related articles to antidiabetic AND Syzygium cumini AND phytochemicals OR bioactive which were published from 2012 to 2023. A systematic search was conducted by using databases Pubmed, Science Direct, Scopus, and Google The inclusion criteria include articles written in English as experimental research or clinical trials, randomized studies, and should contains phytochemical content profiling The exclusion criteria were other type of reports and or irrelevant to aforementioned topic. This review was written according to the reporting items guidelines for systematic payments and Meta-analysis (PRISMA), and SYRCLE's risk of bias (RoB) tool. By the systematic compensation used, 13 articles that meet the requirements were obtained and were further thoroughly reviewed. Seeds, leaves and bark of plant parts were reported to non-toxic in acute experiments on mice or rat. S. cumini contains flavonol glycosides, especially myricetin, myricitrin, quercetin, and kaempferol; phenolics, such as ellagic acid, tannins, gallic acid; alkaloids and saponins. These compounds contribution to the overall anti-diabetic activity were discussed, i.e increase of insulin sensitivity, secretion and usage of glucose in tissues; reducing insulin resistance, oxidative stress, gluconeogenesisand absorption of carbohydrates and sucrose. The seeds and leaves of S. cumini exhibit high potential to be developed as herbal products for anti-diabetics. This review finds a gap in research to support the development of the herbs as herbal products for anti-diabetics, in form of established preclinical and clinical trials and a reliable analytical methods for the phytochemical profiling.","PeriodicalId":13520,"journal":{"name":"INDONESIAN JOURNAL OF PHARMACY","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2023-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139354929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ingrid Faustine, Amarila Malik, Retnosari Andrajati, Septelia Inawati Wanandi
The high activity of Angiotensin Converting Enzyme (ACE) in patients with hypertension is thought to affect the risk and severity of COVID-19. Insertion/deletion (I/D) and SNP polymorphisms of the ACE gene can affect ACE activity in the Renin-Angiotensin System (RAS). This study aims to identify linkage disequilibrium SNPs rs4331 (A/G), rs4341 (G/C), and rs4343 (G/A) with I/D polymorphisms reported in previous studies and their associations with the severity of COVID-19 with hypertension comorbid. A cross-sectional study in 2021 recruited 95 samples of COVID-19 patients from two regions representing the eastern and western parts of Indonesia. Detection of polymorphisms using rhAmp SNP showed the percentage of genotypes GG, AG, and AA at rs4331 according to genotype II, DI, and DD in polymorphism I/D (57%, 32%, and 11%). In contrast, rs4341 only showed one type of genotype (GG), and 37% of the samples were undetermined at rs4343. Based on the linkage disequilibrium, it is known that rs4331, rs1799752, and rs4343 are in strong LD (D'= 0.97 and 0.99), and rs4331 and rs1799752 can be markers of genes to replace one with another (R2= 0.957). The haplotype analysis showed a significant difference between the residents of western and eastern Indonesia (p = 0.005), whereas this was not the case with hypertension comorbidities and the severity of COVID-19. Seventeen percent of the G-I-G-G haplotypes showed moderate-severe severity, while 15% of the A-D-G-G haplotypes showed mild severity. Therefore, it can be concluded that the high I/D, rs4331, and rs4343 polymorphisms are likely co-inherited and that the G-I-G-G haplotype is associated with moderate-to-severe severity in COVID-19.
{"title":"Association Of Single Nucleotide Polymorphism With Insertion/Deletion ACE Gene Detected Using RhAmp Platform In COVID-19 And Hypertension Patients","authors":"Ingrid Faustine, Amarila Malik, Retnosari Andrajati, Septelia Inawati Wanandi","doi":"10.22146/ijp.5647","DOIUrl":"https://doi.org/10.22146/ijp.5647","url":null,"abstract":"The high activity of Angiotensin Converting Enzyme (ACE) in patients with hypertension is thought to affect the risk and severity of COVID-19. Insertion/deletion (I/D) and SNP polymorphisms of the ACE gene can affect ACE activity in the Renin-Angiotensin System (RAS). This study aims to identify linkage disequilibrium SNPs rs4331 (A/G), rs4341 (G/C), and rs4343 (G/A) with I/D polymorphisms reported in previous studies and their associations with the severity of COVID-19 with hypertension comorbid. A cross-sectional study in 2021 recruited 95 samples of COVID-19 patients from two regions representing the eastern and western parts of Indonesia. Detection of polymorphisms using rhAmp SNP showed the percentage of genotypes GG, AG, and AA at rs4331 according to genotype II, DI, and DD in polymorphism I/D (57%, 32%, and 11%). In contrast, rs4341 only showed one type of genotype (GG), and 37% of the samples were undetermined at rs4343. Based on the linkage disequilibrium, it is known that rs4331, rs1799752, and rs4343 are in strong LD (D'= 0.97 and 0.99), and rs4331 and rs1799752 can be markers of genes to replace one with another (R2= 0.957). The haplotype analysis showed a significant difference between the residents of western and eastern Indonesia (p = 0.005), whereas this was not the case with hypertension comorbidities and the severity of COVID-19. Seventeen percent of the G-I-G-G haplotypes showed moderate-severe severity, while 15% of the A-D-G-G haplotypes showed mild severity. Therefore, it can be concluded that the high I/D, rs4331, and rs4343 polymorphisms are likely co-inherited and that the G-I-G-G haplotype is associated with moderate-to-severe severity in COVID-19.","PeriodicalId":13520,"journal":{"name":"INDONESIAN JOURNAL OF PHARMACY","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136221753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Akhirta Atikana, Linda Sukmarini, Mega Ferdina Warsito, Febriana Untari, Tutik Murniasih, Siti Irma Rahmawati, Lailatul Qodria, Olga Rakha Siwi, Shanti Ratnakomala, Anggia Prasetyoputri, Masteria Yunovilsa Putra, Puspita Lisdiyanti
The phylum Actinobacteria recognized as the most promising producers of many bioactive compounds. Among actinobacteria, the genus Streptomyces are widely known as prolific producers of many antibiotics, for example Streptomycin. The present study investigates potential bioactivity of actinobacteria BTA 1-131 isolated from an Indonesian marine sponge Melophlus sarassinorum. Molecular identification using the 16S rRNA gene sequencing showed that the isolate is similar to Streptomyces kunmingensis (98.4%). The isolate was cultivated on to three different fermentation media (ISP2, YS, and SYP) to select the best media for bioactive compounds production. The compounds were extracted from both solid and liquid cultures using methanol as a solvent. The crude methanol extracts were tested for antibacterial and anticancer activity. The bioactivity screening indicates active potential of the extracts against bacterial pathogen Staphylococcus aureus ATCC 13420, invasive breast cancer MDA-MB-231 and human colorectal adenocarcinoma cells CaCo-2. The extracts profiling using LC-MS/MS method indicates probable influence among different fermentation media to selectively induce production of target bioactive compounds. Although further compounds dereplication and fractionation are important for specific identification, the present study demonstrates the potential of Streptomyces BTA 1-131 as producer of tetracycline and/or staurosporine. In addition to that, further genome-mining approach and analysis using a whole genome sequencing data also important to comprehensively explore the bioactive potential of the actinobacteria BTA 1-131.
{"title":"Bioactivity Profiles of Actinobacterium Strain BTA 1-131 (InaCC A1205) Isolated from Indonesian Sponge Melophlus sarassinorum","authors":"Akhirta Atikana, Linda Sukmarini, Mega Ferdina Warsito, Febriana Untari, Tutik Murniasih, Siti Irma Rahmawati, Lailatul Qodria, Olga Rakha Siwi, Shanti Ratnakomala, Anggia Prasetyoputri, Masteria Yunovilsa Putra, Puspita Lisdiyanti","doi":"10.22146/ijp.6455","DOIUrl":"https://doi.org/10.22146/ijp.6455","url":null,"abstract":"The phylum Actinobacteria recognized as the most promising producers of many bioactive compounds. Among actinobacteria, the genus Streptomyces are widely known as prolific producers of many antibiotics, for example Streptomycin. The present study investigates potential bioactivity of actinobacteria BTA 1-131 isolated from an Indonesian marine sponge Melophlus sarassinorum. Molecular identification using the 16S rRNA gene sequencing showed that the isolate is similar to Streptomyces kunmingensis (98.4%). The isolate was cultivated on to three different fermentation media (ISP2, YS, and SYP) to select the best media for bioactive compounds production. The compounds were extracted from both solid and liquid cultures using methanol as a solvent. The crude methanol extracts were tested for antibacterial and anticancer activity. The bioactivity screening indicates active potential of the extracts against bacterial pathogen Staphylococcus aureus ATCC 13420, invasive breast cancer MDA-MB-231 and human colorectal adenocarcinoma cells CaCo-2. The extracts profiling using LC-MS/MS method indicates probable influence among different fermentation media to selectively induce production of target bioactive compounds. Although further compounds dereplication and fractionation are important for specific identification, the present study demonstrates the potential of Streptomyces BTA 1-131 as producer of tetracycline and/or staurosporine. In addition to that, further genome-mining approach and analysis using a whole genome sequencing data also important to comprehensively explore the bioactive potential of the actinobacteria BTA 1-131.
","PeriodicalId":13520,"journal":{"name":"INDONESIAN JOURNAL OF PHARMACY","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135364657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to measure antioxidant activity, to characterize biochemical properties and to measure cytotoxicity activity in BETD5 isolates. The method used in this research was experimental method. Parameters observed were antioxidant activity and cytotoxicity activity consisting of IC50 (Inhibition Concentration) and biochemical properties. Isolate used was extract of endophytic bacteria of Tapak Dara (BETD5). antioxidant activity was conducted using various sample extract concentration, namely 5ppm, 10ppm, 25ppm, 50ppm and 100ppm. The biochemical tests carried out included Triple Sugar Iron Agar (TSIA) test, Simmons Citrate (SC) test, motility test, indole test, catalase test and Methyl Red (MR) test. Cytotoxicity test on cell line T47D was performed by MTT assay using concentration (%) of 3.125; 6.25; 12.5; 25 and 50. The results showed that antioxidant activity of BETD5 was strong because IC50 value obtained was 78.485 ppm. Based on biochemical tests, BETD5 was suspected belong to the genus Staphylococcus. Cytotoxicity activity of BETD5 obtained an IC50 value of 15.58 ppm. This indicates that endophytic bacterial extract of Tapak Dara (BETD5) had moderate cytotoxicity against the T47D breast cancer cell line. GCMS test results showed that the endophytic bacterial isolate BETD5 had the highest secondary metabolite content, namely cis-Ocimene (24.31%).
{"title":"The Potential of Tapak Dara (Catharanthus roseus) Leaves Endophytic Bacteria BETD5 as Antioxidant and Anticancer Against T47D Breast Cancer Cells","authors":"Lenni Fitri, Fauziah Fauziah, Fajar Dini, Sinta Ayu Mauludin, Sasmiati Farach Dita","doi":"10.22146/ijp.5667","DOIUrl":"https://doi.org/10.22146/ijp.5667","url":null,"abstract":"This study aimed to measure antioxidant activity, to characterize biochemical properties and to measure cytotoxicity activity in BETD5 isolates. The method used in this research was experimental method. Parameters observed were antioxidant activity and cytotoxicity activity consisting of IC50 (Inhibition Concentration) and biochemical properties. Isolate used was extract of endophytic bacteria of Tapak Dara (BETD5). antioxidant activity was conducted using various sample extract concentration, namely 5ppm, 10ppm, 25ppm, 50ppm and 100ppm. The biochemical tests carried out included Triple Sugar Iron Agar (TSIA) test, Simmons Citrate (SC) test, motility test, indole test, catalase test and Methyl Red (MR) test. Cytotoxicity test on cell line T47D was performed by MTT assay using concentration (%) of 3.125; 6.25; 12.5; 25 and 50. The results showed that antioxidant activity of BETD5 was strong because IC50 value obtained was 78.485 ppm. Based on biochemical tests, BETD5 was suspected belong to the genus Staphylococcus. Cytotoxicity activity of BETD5 obtained an IC50 value of 15.58 ppm. This indicates that endophytic bacterial extract of Tapak Dara (BETD5) had moderate cytotoxicity against the T47D breast cancer cell line. GCMS test results showed that the endophytic bacterial isolate BETD5 had the highest secondary metabolite content, namely cis-Ocimene (24.31%).","PeriodicalId":13520,"journal":{"name":"INDONESIAN JOURNAL OF PHARMACY","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135286985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Olena Grechana, Anatoly Serbin, Anna Rudnik, Olena Saliy
Alfalfa (Medicago sativa L., Fabaceae) is knowed as perennial herbaceous leguminous plant species that originated in southwestern Asia and used as folk medicine for the treatment of various ailments. The upperground part of Lucerne contain phenolic compound such as flavonoids etc., that contributes to its biological activities. The aim of this study was to determine five widely knowing flavonoids in extracts 20 alfalfa varieties herb at the Ukrainian steppe growing. We selected 50 seeds of the same size from twenty alfalfa cultivars from different countries, were cultivated under controlled areas of southern part of the Ukrainian left-bank at border of forest-steppe and steppe zones (Zaporizhzhya, Ukraine) from April to June, with 15 °C/ 07 °C (day/night), 14 h/10 h (light/dark) and 60–65% relative humidity. The content of flavonoids were found unequable in ethanol extracts. The chemical compositions and their content were assessed by ultrahigh-performance liquid chromatography. The content of five flavonoids were different in the 20 alfalfa varieties raw materials. Umbelliferone were found high in ethanol extract of Mongolian colorful hybrid (Mongolia, 0,23 mg|g). Four sorts have not contained umbelliferone: Кisvardai (Hungary), Nizona (Cuba), Тanhuato (Mexico) and Mesopotamian (Iraq). The leader from cinaroside content was sort Commercial 2-52-75 of UK origin. Routine has been found in the highest quantities in WL 50 from USA. Ferganska 700 from Uzbekistan was leader in luteolin content and Кisvardai, Hungary was leader in average of kaempferol content (0,030 mg|g). The present article comprises the hierarchical cluster analyses from the data flavonoid assay.
{"title":"Five flavonoids from Lucerne (Medicago sativa L.) varieties","authors":"Olena Grechana, Anatoly Serbin, Anna Rudnik, Olena Saliy","doi":"10.22146/ijp.3671","DOIUrl":"https://doi.org/10.22146/ijp.3671","url":null,"abstract":"Alfalfa (Medicago sativa L., Fabaceae) is knowed as perennial herbaceous leguminous plant species that originated in southwestern Asia and used as folk medicine for the treatment of various ailments. The upperground part of Lucerne contain phenolic compound such as flavonoids etc., that contributes to its biological activities. The aim of this study was to determine five widely knowing flavonoids in extracts 20 alfalfa varieties herb at the Ukrainian steppe growing. We selected 50 seeds of the same size from twenty alfalfa cultivars from different countries, were cultivated under controlled areas of southern part of the Ukrainian left-bank at border of forest-steppe and steppe zones (Zaporizhzhya, Ukraine) from April to June, with 15 °C/ 07 °C (day/night), 14 h/10 h (light/dark) and 60–65% relative humidity. The content of flavonoids were found unequable in ethanol extracts. The chemical compositions and their content were assessed by ultrahigh-performance liquid chromatography. The content of five flavonoids were different in the 20 alfalfa varieties raw materials. Umbelliferone were found high in ethanol extract of Mongolian colorful hybrid (Mongolia, 0,23 mg|g). Four sorts have not contained umbelliferone: Кisvardai (Hungary), Nizona (Cuba), Тanhuato (Mexico) and Mesopotamian (Iraq). The leader from cinaroside content was sort Commercial 2-52-75 of UK origin. Routine has been found in the highest quantities in WL 50 from USA. Ferganska 700 from Uzbekistan was leader in luteolin content and Кisvardai, Hungary was leader in average of kaempferol content (0,030 mg|g). The present article comprises the hierarchical cluster analyses from the data flavonoid assay.","PeriodicalId":13520,"journal":{"name":"INDONESIAN JOURNAL OF PHARMACY","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135429134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A scientific method is essential for phytochemists to deliver active ingredients in a consistent manner. This can be accomplished through the development of novel drug delivery systems for active plant compounds. The current study aims to prepare Annona muricata leaves extract (ALE) containing nano zinc formulation into a nano-particulate hydrogel. The active components were extracted by cold maceration of Anona muricata leaves in ethanol and then converted into polymeric nanoparticles by gelation method based on electrostatic interaction between the Hydroxyl Amine on Chitosan and a cluster of negative charge from the polyanion NaTPP. Zinc oxide nano particles (ZNPs) was formulated by precipitating zinc acetate with sodium hydroxide. These were then incorporated with Carbopol-940 to generate the gel matrix. The result demonstrated that polymeric nanoparticles are closely spherical in shape with a z-average 358.45 ± 11.3 nm and PDI in the range of 0.440-0.812. The gels exhibited a high viscosity, a neutral pH, and acceptable spreadability. Furthermore, a stability assay demonstrates that it has chemical stability and does not cause skin irritation, making it appropriate for transdermal delivery.
{"title":"Formulation and Evaluation of Topical Nano-Hydrogel of Zinc and Annona Muricata Extract","authors":"Muhtadi Muhtadi, Eko Mugiyanto, Nuniek Nizmah Fajriyah, Slamet S, Urmatul Waznah, Dwi Bagus Pambudi, None Fridiana, Eka Dian Safitri, Fahrun Nur Rosyidand","doi":"10.22146/ijp.5788","DOIUrl":"https://doi.org/10.22146/ijp.5788","url":null,"abstract":"A scientific method is essential for phytochemists to deliver active ingredients in a consistent manner. This can be accomplished through the development of novel drug delivery systems for active plant compounds. The current study aims to prepare Annona muricata leaves extract (ALE) containing nano zinc formulation into a nano-particulate hydrogel. The active components were extracted by cold maceration of Anona muricata leaves in ethanol and then converted into polymeric nanoparticles by gelation method based on electrostatic interaction between the Hydroxyl Amine on Chitosan and a cluster of negative charge from the polyanion NaTPP. Zinc oxide nano particles (ZNPs) was formulated by precipitating zinc acetate with sodium hydroxide. These were then incorporated with Carbopol-940 to generate the gel matrix. The result demonstrated that polymeric nanoparticles are closely spherical in shape with a z-average 358.45 ± 11.3 nm and PDI in the range of 0.440-0.812. The gels exhibited a high viscosity, a neutral pH, and acceptable spreadability. Furthermore, a stability assay demonstrates that it has chemical stability and does not cause skin irritation, making it appropriate for transdermal delivery.","PeriodicalId":13520,"journal":{"name":"INDONESIAN JOURNAL OF PHARMACY","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135674364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sappan wood is a medicinal plant that contains phenolic compounds such as brazilin. It can be used to treat photoaging due to oxidative stress. Face tonic is one type of facial skin care cosmetic that has this purpose. The research aims to determine the formula, IC50 value, and irritation potency of face tonic from the ethanol fraction of sappan wood. The ethanol fraction of sappan wood was formulated into a face tonic preparation using glycerin and propylene glycol as cosolvents. The evaluation of face tonic involved antioxidant activity using the DPPH method. Meanwhile, the irritation-potential test was conducted using the HET-CAM method. The results showed that face tonic formulas had an antioxidant activity with IC50 values of 639.904 ppm (FI) and 620.365 ppm (FII). The irritation score of each formula was 0 (non-irritant) and 5.44 (moderate irritant). Face tonic with glycerin as a cosolvent has antioxidant activity and is safe to use.
{"title":"Antioxidant Activity and Irritation Potency of Face Tonic Formulation from Ethanol Fraction of Sappan Wood (Caesalpinia sappan L.)","authors":"Dina Yuspita Sari, Ratna Widyasari","doi":"10.22146/ijp.4552","DOIUrl":"https://doi.org/10.22146/ijp.4552","url":null,"abstract":"Sappan wood is a medicinal plant that contains phenolic compounds such as brazilin. It can be used to treat photoaging due to oxidative stress. Face tonic is one type of facial skin care cosmetic that has this purpose. The research aims to determine the formula, IC50 value, and irritation potency of face tonic from the ethanol fraction of sappan wood. The ethanol fraction of sappan wood was formulated into a face tonic preparation using glycerin and propylene glycol as cosolvents. The evaluation of face tonic involved antioxidant activity using the DPPH method. Meanwhile, the irritation-potential test was conducted using the HET-CAM method. The results showed that face tonic formulas had an antioxidant activity with IC50 values of 639.904 ppm (FI) and 620.365 ppm (FII). The irritation score of each formula was 0 (non-irritant) and 5.44 (moderate irritant). Face tonic with glycerin as a cosolvent has antioxidant activity and is safe to use.
","PeriodicalId":13520,"journal":{"name":"INDONESIAN JOURNAL OF PHARMACY","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135674365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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