Pub Date : 2018-07-23DOI: 10.26418/indonesian.v1i1.26038
V. A. Fabiani
Silica gel synthesis from glass waste have been carried out with activation and purification method by using hydrochloride acid. Hydrochloride acid were varied 8 N, 10 N, and 12 N to observe optimum concentration in purification of silica gel and component separation in chromatography column. Gravimetric analysis result showed 12 N hydrochloride acid produce ~100 % silica. XRD and FTIR analysis revealed that synthetic gel silica was amorphous where its IR absorption bands were at 3426 cm-1, 1620 cm-1, 1080 cm-1, 772 cm-1 and 478 cm-1. The absorption bands agreed to standard gel silica and spectrum. The most effective HCl in gel silica synthesis was 12 N, caused by its ability to separate color component in a column. The synthetic silica that can be applied as stationary phase in column chromatography.
{"title":"SINTESIS DAN KARAKTERISASI SILIKA GEL DARI LIMBAH KACA SERTA APLIKASINYA PADA KROMATOGRAFI KOLOM","authors":"V. A. Fabiani","doi":"10.26418/indonesian.v1i1.26038","DOIUrl":"https://doi.org/10.26418/indonesian.v1i1.26038","url":null,"abstract":"Silica gel synthesis from glass waste have been carried out with activation and purification method by using hydrochloride acid. Hydrochloride acid were varied 8 N, 10 N, and 12 N to observe optimum concentration in purification of silica gel and component separation in chromatography column. Gravimetric analysis result showed 12 N hydrochloride acid produce ~100 % silica. XRD and FTIR analysis revealed that synthetic gel silica was amorphous where its IR absorption bands were at 3426 cm-1, 1620 cm-1, 1080 cm-1, 772 cm-1 and 478 cm-1. The absorption bands agreed to standard gel silica and spectrum. The most effective HCl in gel silica synthesis was 12 N, caused by its ability to separate color component in a column. The synthetic silica that can be applied as stationary phase in column chromatography.","PeriodicalId":13554,"journal":{"name":"Indonesian Journal of Pure and Applied Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79929571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-07-23DOI: 10.26418/indonesian.v1i1.26037
H. Hermansyah, Novian Sutami, M. Miksusanti
The purpose of this research was to isolated DNA from the yeast C. tropicalis with freeze thawing method -200 C conducted on 3 colonies of C. tropicalis. Each colony threated variations of cooling, 3x15 minutes, 3x25 minutes and 3x35 minutes, to break the cell walls. Subsequently all the samples amplified with 3 variations of PCR cycles, 15 cycles, 25 cycles and 35 cycles, after all of the samples isolated by freeze thawing method -200 C. Its was known that sample A15 has the smallest concentration of DNA yeast C. tropicalis, ie 50 µg/mL, while sample C35 had the largest concentration of DNA yeast C. tropicalis, ie 225 µg/mL. The result of the research indicated that the best condition can be reached in 3x35 minutes. On 35th cycle has clearer C. tropicalis DNA bands than the 25th and 15th PCR cycle. C. tropicalis DNA bands at 35th cycles there were 7 DNA bands were detected and bright bands on a long 35 minutes cooling. In the 25th and the 15th cycle, there was no DNA bands were detected in all samples. Based on the results obtained, the amplification process must be carried out at least 35 times cycles so that the C. tropicalis DNA bands can be detected.
{"title":"AMPLIFIKASI PCR DOMAIN D1/D2 28S rDNA MENGGUNAKAN PRIMER ITS1 DAN ITS4 SAMPEL DNA DARI Candida tropicalis YANG DIISOLASI DENGAN METODE PENDINGINAN","authors":"H. Hermansyah, Novian Sutami, M. Miksusanti","doi":"10.26418/indonesian.v1i1.26037","DOIUrl":"https://doi.org/10.26418/indonesian.v1i1.26037","url":null,"abstract":"The purpose of this research was to isolated DNA from the yeast C. tropicalis with freeze thawing method -200 C conducted on 3 colonies of C. tropicalis. Each colony threated variations of cooling, 3x15 minutes, 3x25 minutes and 3x35 minutes, to break the cell walls. Subsequently all the samples amplified with 3 variations of PCR cycles, 15 cycles, 25 cycles and 35 cycles, after all of the samples isolated by freeze thawing method -200 C. Its was known that sample A15 has the smallest concentration of DNA yeast C. tropicalis, ie 50 µg/mL, while sample C35 had the largest concentration of DNA yeast C. tropicalis, ie 225 µg/mL. The result of the research indicated that the best condition can be reached in 3x35 minutes. On 35th cycle has clearer C. tropicalis DNA bands than the 25th and 15th PCR cycle. C. tropicalis DNA bands at 35th cycles there were 7 DNA bands were detected and bright bands on a long 35 minutes cooling. In the 25th and the 15th cycle, there was no DNA bands were detected in all samples. Based on the results obtained, the amplification process must be carried out at least 35 times cycles so that the C. tropicalis DNA bands can be detected.","PeriodicalId":13554,"journal":{"name":"Indonesian Journal of Pure and Applied Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88997706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-07-23DOI: 10.26418/indonesian.v1i1.26039
Robby Gus Mahardika, Sito Enggiwanto, Ary Samsiar
Silica Waste cooking oil can be used as a soap or biodiesel. Good soaps or biodiesel should be from oils that have low levels of fatty acids and free radicals. However, waste cooking oil has high free fatty acid and free radical, it is necessary to increase the quality of waste cooking oil. One effort to improve the quality of waste cooking oil can use activated carbon as an adsorbent. Decrease in free radicals in cooking oil can use antioxidants from extract pucuk idat (Cratoxylum glaucum). This study aims to see the effect of extract pucuk idat on the process of improving the quality of waste cooking oil. The process of improvement by adding activated carbon and varying the concentration of ethanol extract pucuk idat. Activated carbon used 10% with variation of extract 0,25%; 0,5% and 0,75%. This process followed by stirring for 15 minutes at 80°C, then soaked for 3 days. Oil quality are identified by the method of determining the levels of free fatty acids and acid numbers. The results of this study indicate that extract pucuk idat in ethanol with 0,75% concentration has the lowest free fatty acid and acid number. Extract pucuk idat can improve the quality of waste cooking oil.
{"title":"PENINGKATAN KUALITAS MINYAK JELANTA MENGGUNAKAN KARBON AKTIF DAN EKSTRAK PUCUK IDAT (Cratoxylum glaucum)","authors":"Robby Gus Mahardika, Sito Enggiwanto, Ary Samsiar","doi":"10.26418/indonesian.v1i1.26039","DOIUrl":"https://doi.org/10.26418/indonesian.v1i1.26039","url":null,"abstract":"Silica Waste cooking oil can be used as a soap or biodiesel. Good soaps or biodiesel should be from oils that have low levels of fatty acids and free radicals. However, waste cooking oil has high free fatty acid and free radical, it is necessary to increase the quality of waste cooking oil. One effort to improve the quality of waste cooking oil can use activated carbon as an adsorbent. Decrease in free radicals in cooking oil can use antioxidants from extract pucuk idat (Cratoxylum glaucum). This study aims to see the effect of extract pucuk idat on the process of improving the quality of waste cooking oil. The process of improvement by adding activated carbon and varying the concentration of ethanol extract pucuk idat. Activated carbon used 10% with variation of extract 0,25%; 0,5% and 0,75%. This process followed by stirring for 15 minutes at 80°C, then soaked for 3 days. Oil quality are identified by the method of determining the levels of free fatty acids and acid numbers. The results of this study indicate that extract pucuk idat in ethanol with 0,75% concentration has the lowest free fatty acid and acid number. Extract pucuk idat can improve the quality of waste cooking oil.","PeriodicalId":13554,"journal":{"name":"Indonesian Journal of Pure and Applied Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73626601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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