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Screening of Rice Genotypes against Brown Spot Disease at Rampur, Chitwan, Nepal 尼泊尔奇特旺兰普尔水稻褐斑病基因型筛选
Pub Date : 2023-08-10 DOI: 10.20546/ijcmas.2023.1208.006
Sanjok Timalsina
Brown spot of rice caused by Bipolaris oryzae (Breda de. Haan) Shoemaker is one of the major constraints of rice production in all rice growing regions of Nepal. To identify and select the sources of brown spot resistance in rice genotypes, a field experiment was conducted under the natural epiphytotic condition at National Maize Research Center, Rampur, Chitwan in 2022. A total of 10 genotypes, including susceptible check (Sabha Mansuli SUB-1) and resistant check (Sabitri) were tested. The field experiment was laid out in a randomized complete block design with three replications. Brown spot disease assessment was done based on the percent of leaf area diseased according to the 0-9 scales of the standard evaluation system. The percent disease severity was recorded at 50, 60, 70, and 80 days after transplanting (DAT). It was observed that the disease severity increased with an increase in the age of the crop from 50-80 DAT. Disease severity and the total AUDPC values were highly significant among the rice genotypes. Disease severity and total AUDPC value ranged from 28.52% to 39.42% and 853.33 to 1185.19 respectively. None of these genotypes was found either resistant or highly resistant to disease. The mean severity and total AUDPC values of the compared genotypes were significantly lower than Sabha Mansuli SUB-1. The Genotypes NR2181-465-1-1-1-1, NR2264-4-1-6-5, NR2215-6-4-2-2-2, NR2191-22-1-4-1-1, and NR2191-236-3-1-3-1 demonstrated mean severity and total AUDPC in increasing order but lesser than that of Sabitri. NR2181-465-1-1-1-1 had the lowest disease severity (28.52%) as well as total AUDPC value (853.33). From this experiment, it can be concluded that under similar field conditions, NR2181-465-1-1-1-1 genotype having lower mean disease severity and total AUDPC can be used for further multi location trials for resistance evaluation.
由 Bipolaris oryzae (Breda de. Haan) Shoemaker 引起的水稻褐斑病是尼泊尔所有水稻种植区水稻生产的主要制约因素之一。为了鉴定和选择水稻基因型的褐斑病抗性来源,2022 年,在奇特旺省兰普尔的国家玉米研究中心进行了一次自然附生条件下的田间试验。共测试了 10 个基因型,包括易感对照(Sabha Mansuli SUB-1)和抗性对照(Sabitri)。田间试验采用随机完全区组设计,三次重复。褐斑病的评估是根据标准评估系统 0-9 级的患病叶面积百分比进行的。在移栽后 50、60、70 和 80 天(DAT)记录病害严重程度百分比。据观察,从 50-80 DAT 天,病害严重程度随着作物年龄的增加而增加。不同水稻基因型的病害严重程度和 AUDPC 总值差异很大。病害严重程度和 AUDPC 总值的范围分别为 28.52% 至 39.42% 和 853.33 至 1185.19。这些基因型中没有一个抗病或高抗病。比较基因型的平均严重程度和 AUDPC 总值明显低于 Sabha Mansuli SUB-1。基因型 NR2181-465-1-1-1、NR2264-4-1-6-5、NR2215-6-4-2-2、NR2191-22-1-4-1-1 和 NR2191-236-3-1-3-1 的平均严重程度和总 AUDPC 值依次增加,但低于 Sabitri。NR2181-465-1-1-1 的病害严重程度(28.52%)和 AUDPC 总值(853.33)最低。从该试验中可以得出结论,在类似的田间条件下,NR2181-465-1-1-1 基因型的平均病害严重程度和总 AUDPC 值较低,可用于进一步的多点试验,以进行抗性评估。
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引用次数: 0
Antioxidant Profile of Rats in Mancozeb Induced Toxicity and its Amelioration by Tridax procumbens 代森锰锌诱导毒性大鼠的抗氧化谱及其对代森锰锌毒性的改善作用
Pub Date : 2023-08-10 DOI: 10.20546/ijcmas.2023.1208.003
Debolina Dattaray, Rajdeep Kaur, S. K. Sharma, Sunita Kumawat, M. Lonare
Mancozeb, a di-thiocarbamate fungicide, is widely used in agricultural practices for the control of parasitic fungi and their spores. The present study investigates the blood antioxidant profile of rats following subacute oral mancozeb exposure and the ameliorative effect of aqueous and methanolic extracts of Tridax procumbens on mancozeb induced toxicity in rats. 36 wistar rats were divided into 6 groups of 6 animals, each (Control, Mancozeb (@500mg/kg) treatment group, Tridax aqueous extract (@300mg/kg) group, Tridax methanol extract (@300mg/kg) group, Tridax aq extract + Mancozeb group and Tridax met extract + mancozeb group. The rats received the fungicide and the different extracts of the plant orally for 28 consecutive days, except the control group. Subacute mancozeb exposure significantly elevated the oxidative stress marker LPO. The altered antioxidant status was evident from the depleting blood glutathione levels, significant elevation in enzymic antioxidant parameters (Catalase, GST, G6PD) and simultaneous decrease in enzymes like SOD, GPxand GR. Supplementation of Tridax aq. extract and Tridax met. extract @ 300 mg/kg per day for 28 days in mancozeb intoxicated rats had a beneficial effect on the overall antioxidant profile of the animals as witnessed by no significant alteration in LPO, blood glutathione levels and activities of various antioxidant enzymes viz. GST, GR, SOD, CAT and G6PD. Therefore, the results of the present study suggest that mancozeb administration induces pronounced oxidative stress in the rats and aqueous and methanolic extract of Tridax procumbens have mild to moderate ameliorative effect in the treated rats.
代森锰锌是一种二硫代氨基甲酸酯类杀菌剂,在农业生产中广泛用于控制寄生真菌及其孢子。本研究调查了亚急性口服代森锰锌后大鼠血液中抗氧化剂的情况,以及蒲公英水提取物和甲醇提取物对代森锰锌诱导的大鼠毒性的改善作用。将 36 只大鼠分为 6 组,每组 6 只(对照组、曼可唑(@500 毫克/千克)处理组、踯躅水提取物(@300 毫克/千克)组、踯躅甲醇提取物(@300 毫克/千克)组、踯躅水提取物 + 曼可唑组和踯躅甲醇提取物 + 曼可唑组)。除对照组外,大鼠连续 28 天口服杀真菌剂和不同的植物提取物。亚急性芒硝暴露会显著升高氧化应激标志物 LPO。血液中谷胱甘肽水平的降低、酶抗氧化参数(过氧化氢酶、GST、G6PD)的显著升高以及 SOD、GPx 和 GR 等酶的同时降低都表明抗氧化状态发生了改变。在芒克泽布中毒大鼠中连续 28 天每天补充 300 毫克/千克的踯躅aq.提取物和踯躅met.提取物对动物的整体抗氧化状况有好处,这体现在 LPO、血液谷胱甘肽水平和各种抗氧化酶(即 GST、GR、SOD、CAT 和 G6PD)的活性没有明显改变。因此,本研究的结果表明,给大鼠施用芒硝会诱发明显的氧化应激,而刺五加的水提取物和甲醇提取物对大鼠有轻度至中度的改善作用。
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引用次数: 0
Role of Crisper-Cas Technique in Bioremediation of Pesticides Crisper-Cas 技术在农药生物修复中的作用
Pub Date : 2023-08-10 DOI: 10.20546/ijcmas.2023.1208.010
Manisha Chaudhari, Krunal Solanki
Pesticides, insecticides, certain pharmaceuticals, chemically dangerous chemicals which can pollute the environment. Pesticides are primarily used to manage plants, flies, parasitic fungus, and parasites in crop fields. The desire to limit pesticide effects on soil and offsite environments has encouraged research interest in pesticide and related chemical biodegradation. As a result, quick and safe agents for environmental bioremediation, individual decontamination, and therapeutic detoxication are urgently needed. Bioremediation is environment friendly so if we use such kind of technique then more helpful. The ability of microorganisms to degrade complex chemical substances in the environment is referred to as bioremediation. The discovery of CRISPR, mechanism of the CRISPR-based nuclear adaptive immune system "CRISPR associated system, Cas", and its diversion to powerful tools for gene editing revolutionize the field of molecular biology. Brought about and stimulated new and improved gene therapy. By using CRISPR technique transfer desired gene and decrease toxic elements.
杀虫剂、杀虫剂、某些药品,这些化学危险品会污染环境。杀虫剂主要用于管理农田中的植物、苍蝇、寄生真菌和寄生虫。为了限制杀虫剂对土壤和外部环境的影响,人们对杀虫剂和相关化学品的生物降解研究产生了浓厚的兴趣。因此,迫切需要快速、安全的环境生物修复、个人净化和治疗解毒剂。生物修复技术对环境友好,因此,如果我们使用这种技术,会更有帮助。微生物降解环境中复杂化学物质的能力被称为生物修复。CRISPR的发现、基于CRISPR的核适应性免疫系统 "CRISPR相关系统Cas "的机理以及将其转化为强大的基因编辑工具,彻底改变了分子生物学领域。带来并刺激了新的改良基因疗法。利用 CRISPR 技术转移所需的基因,减少有毒元素。
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引用次数: 0
Seed Mycoflora of Sesame (Sesamum indicum L.) and their Phytopathogenic Effect 芝麻(Sesamum indicum L.)种子霉菌群及其植物病原效应
Pub Date : 2023-08-10 DOI: 10.20546/ijcmas.2023.1208.007
A. T. Arun, Mahabeer Singh
Sesame (Sesamum indicum L.) which belongs to the pedaliaceae family, is one of the oldest oilseed crops cultivated in tropical and subtropical regions of Asia, Africa and South America. India ranks first in both acreage and production (about 8 lakh MT) of sesame in the world. The seed mycoflora which causes deteriorative effects like reduction in seed quality and their germination in sesame. Seed samples of sesame were collected from five tehasils of Jaipur district, revealed variation in germination, seedling symptoms and incidence of seed mycoflora which finally effect seed quality. Discolouration (dirty black), deformity (Shrivelled), along with damaged seeds and impurities were commonly found in all the seed samples. A total number of eight seed mycoflora viz., Alternaria sesami, Aspergillus flavus, Aspergillus niger, Colletotrichum spp., Fusarium spp., Macrophomina phaseolina, Mucor spp. and Penicillium spp. were obtained in both Blotter and Agar Plate Method. The disease incidence of Alternaria sesami were found predominant in among the inoculation method. Maximum disease incidence of mycoflora was observed in seed sample ‘A’ whereas minimum observed in seed sample ‘C‘. Among these, Alternaria sesami was found to be highly pathogenic as it showed maximum reduction in seed germination and vigour index with enhanced pre and post-emergence mortality. The pathogenicity of Alternaria sesami highest was observed in seed cum foliar inoculation technique.
芝麻(Sesamum indicum L.)属于豆科植物,是亚洲、非洲和南美洲热带和亚热带地区种植的最古老的油籽作物之一。印度的芝麻种植面积和产量(约 80 万公吨)均居世界首位。种子霉菌群会导致芝麻种子质量和发芽率下降。从斋浦尔地区的五个村庄收集的芝麻种子样本显示,发芽率、幼苗症状和种子霉菌的发生率都存在差异,这些最终都会影响种子的质量。所有种子样本中都普遍存在变色(脏黑)、畸形(干瘪)、种子破损和杂质。在印迹法和琼脂平板法中,共获得了 8 种种子霉菌,即芝麻间苯二酚、黄曲霉、黑曲霉、壳针孢属、镰刀菌属、相思豆属、粘孢属和青霉属。在各种接种方法中,芝麻赤霉病的发病率最高。种子样本 "A "中霉菌的发病率最高,而种子样本 "C "中发病率最低。在这些病原菌中,芝麻交链孢属病原菌的致病性很强,因为它最大程度地降低了种子的发芽率和活力指数,并增加了萌发前和萌发后的死亡率。在种子和叶面接种技术中观察到的芝麻赤霉病致病性最高。
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引用次数: 0
Quantification of Biofilm Forming Ability of Salmonella species at Different Time Interval 不同时间间隔下沙门氏菌生物膜形成能力的定量分析
Pub Date : 2023-08-10 DOI: 10.20546/ijcmas.2023.1208.016
N. S. Rakkith, C. B. Madhavaprasad, Prashanth S. Bagalakote, S. J. Arun, K. G. Udupa, L. R. Ananthakrishna, P. Hemanth, B. Jithendra
The ability of 12 Salmonella isolates originating from animal, birds and environmental sources to form biofilms was investigated using 96-well flat microtitre plate assay. At 24-hours, none of the isolates formed biofilm, while most isolates were weak biofilm producers at 48 and 72 hours. As the biofilms has the ability to resist disinfectants, antimicrobials and contribute to virulence, has gained importance in food industries.
使用 96 孔平板微滴定板测定法研究了 12 种来自动物、鸟类和环境的沙门氏菌分离物形成生物膜的能力。在 24 小时内,没有一个分离菌株形成生物膜,而在 48 小时和 72 小时内,大多数分离菌株的生物膜生成能力较弱。由于生物膜具有抵抗消毒剂和抗菌剂的能力,并能增强毒性,因此在食品工业中越来越重要。
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引用次数: 0
Lithophytic Cyanobacteria on Indian Stone Temples and Monuments 印度石庙和石碑上的岩生蓝藻
Pub Date : 2023-08-10 DOI: 10.20546/ijcmas.2023.1208.019
Rashmi Kala, V. D. Pandey
India is rich in lithic (stone-built) temples and monuments which epitomize our rich cultural heritage and contribute significantly to the tourism and economy of the country. The light-exposed surfaces of temples and monuments are readily colonized and inhabited by various species, belonging to different genera, of lithophytic (lithobiontic) cyanobacteria (blue-green algae). Lithophytic cyanobacteria possess remarkable adaptability and tolerance to various abiotic stresses, such as desiccation, high light intensity, high levels of solar UV radiation and high temperature which they often encounter on exposed rock surfaces and external walls of lithic temples, monuments and buildings. They are primary colonizers of nutrient-poor lithic substrata. Lithophytic cyanobacteria can grow both as epiliths and endoliths. They comprise major component of sub-aerial biofilms or crusts on exposed surfaces.The colonization and growth of lithophytic cyanobacteria can affect stone-built temples, monuments and buildings directly or indirectly in various ways, ultimately resulting in their biodeterioration that is manifested as both aesthetic and structural damage. Biodeterioration of stone-built monuments and buildings is a serious problem globally. The article provides an overview of the occurrence and diversity of lithophytic cyanobacteria on Indian stone-built temples and monuments and their potential effects.
印度拥有丰富的石质(石砌)寺庙和古迹,它们是我国丰富文化遗产的缩影,为印度的旅游业和经济做出了巨大贡献。寺庙和古迹暴露在光线下的表面很容易被属于不同属的各种石生蓝藻(蓝绿藻)定殖和栖息。岩生蓝藻对各种非生物压力(如干燥、高光照强度、高水平的太阳紫外线辐射和高温)具有极强的适应性和耐受性,它们经常在裸露的岩石表面和石质寺庙、纪念碑和建筑的外墙上遇到这些压力。它们是营养贫乏的岩石基质的主要定殖者。石生蓝藻既能以附生体的形式生长,也能以内生体的形式生长。石生蓝藻的定殖和生长会以各种方式直接或间接地影响石砌寺庙、纪念碑和建筑物,最终导致其生物劣化,表现为美观和结构破坏。石砌古迹和建筑的生物劣化是一个全球性的严重问题。本文概述了印度石砌寺庙和古迹中石生蓝藻的发生、多样性及其潜在影响。
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引用次数: 0
Environmental Advocacy Groups' Impact and Strategies in Waste Management: Shaping Policies and Raising Awareness, with a Focus on Food Waste 环保倡导团体在废物管理方面的影响和策略:以食物垃圾为重点,制定政策和提高认识
Pub Date : 2023-08-10 DOI: 10.20546/ijcmas.2023.1208.013
Aligina Anvitha Sudheshna, Mamta Sharma, Sunita Agarwal
Waste management is an increasingly critical environmental issue, necessitating concerted efforts to address its impact on ecosystems and human well-being. Environmental advocacy groups play a pivotal role in driving sustainable waste management policies and raising awareness about pressing challenges, such as food waste. This review paper examines the influence and strategies of environmental advocacy groups in waste management, with a specific focus on their impact on policies and initiatives and their role in combatting food waste. The concept of environmental advocacy groups and their role in waste management were discussed. Through case studies and examples, an analysis was drawn on how these groups have successfully influenced waste management policies at various levels, ranging from local communities to international forums. Moreover, it explores the strategies employed by advocacy groups, including social media campaigns, public awareness initiatives, lobbying efforts, grassroots movements, and collaborations with diverse stakeholders. With a special emphasis on food waste, the review paper unveils the role of environmental advocacy groups in tackling critical issues. The paper discusses the far-reaching implications of food waste, encompassing economic losses, social disparities, and environmental degradation. Leveraging innovative strategies will be instrumental in driving positive change.
废物管理是一个日益严峻的环境问题,需要共同努力解决其对生态系统和人类福祉的影响。环保倡导团体在推动可持续废物管理政策和提高人们对食物浪费等紧迫挑战的认识方面发挥着关键作用。本综述文件探讨了环保倡导团体在废物管理方面的影响和策略,重点关注其对政策和倡议的影响,以及在打击食物浪费方面的作用。文中讨论了环保倡导团体的概念及其在废物管理中的作用。通过案例研究和举例,分析了这些团体如何成功地影响从地方社区到国际论坛等各个层面的废物管理政策。此外,报告还探讨了倡导团体所采用的策略,包括社交媒体活动、提高公众意识倡议、游说工作、基层运动以及与不同利益相关者的合作。本文特别强调了食物浪费问题,揭示了环保倡导团体在解决关键问题中的作用。文件讨论了食物浪费的深远影响,包括经济损失、社会差距和环境退化。利用创新战略将有助于推动积极的变革。
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引用次数: 0
Application of Cold Plasma in Food Science: A Novel Technique 冷等离子体在食品科学中的应用:一种新技术
Pub Date : 2023-08-10 DOI: 10.20546/ijcmas.2023.1208.017
Kajal Srivastava, Namrata Habiba Begum
Fresh and safe food is the demand of customers in past decade, to fulfill this demand various novel technologies are being employed on food, one such technique is being discussed in this review i.e., Cold Plasma. Recently cold plasma has proven to be a budding technique primarily in food preservation sector. The main highlight of this technique is minimum heat damage, also the processing time to kill pathogens is considerably low as compared to other techniques. It is being utilized in killing pathogenic micro-organisms in various sectors of food. In this review we will see the mechanism behind generation of cold plasma as well as its types. The paper also summarizes its effect on different food sectors such as milk, meat, packaging, fruits, vegetables etc. Cold plasma is also being utilized in altering the food components as well as packaging materials. Despite being a promising option, the technique still is under scrutiny due to challenges faced by researchers which will be discussed briefly.
在过去的十年中,新鲜和安全的食品一直是客户的需求,为了满足这一需求,各种新型技术被应用到食品中,本综述将讨论其中的一种技术,即冷等离子体。最近,冷等离子体已被证明是食品保鲜领域的一项新兴技术。这种技术的主要亮点是热损伤最小,与其他技术相比,杀死病原体的处理时间也大大缩短。在食品的各个领域,它都被用来杀灭病原微生物。在这篇综述中,我们将了解冷等离子体产生的机理及其类型。本文还总结了冷等离子体对牛奶、肉类、包装、水果、蔬菜等不同食品行业的影响。冷等离子体还被用于改变食品成分和包装材料。尽管冷等离子技术前景广阔,但由于研究人员面临的挑战,该技术仍受到严格审查,本文将对此进行简要讨论。
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引用次数: 0
Production and Problems in Large Cardamom Cultivation in the Sub- Himalayan Region of West Bengal 西孟加拉邦次喜马拉雅地区大面积种植小豆蔻的产量和问题
Pub Date : 2023-08-10 DOI: 10.20546/ijcmas.2023.1208.004
Deparna Pradhan, Gunja Kumari, Kalyan Kanti Das
Large cardamom (Amomum subulatum), locally known as ‘alaichi’, is commonly referred to as the queen of spices. India happens to be the second largest producer of this age old spice and shares about 37% of its global production. The present study attempts to analyze the financial & economic performance of Large Cardamom cultivation with the help of primary data collected from 60 randomly selected sample farmers from Kalimpong district of West Bengal during 2019-2020. It is cultivated as one of the most important cash crops in the study area. Garrett’s ranking technique is used to identify the major constraints faced during production of large cardamom. Discounted method of financial feasibility analysis explores Benefit-Cost Ratio (BCR) to be 3.01 and Net Present Value (NPV) to be ₹1, 99,929.92 (per ha per annum) at 7 percent discount rate confirming the financial viability and profitability of large cardamom cultivation. It is found that the crop exhibits upward trend in yield with its age and attains the ‘maxima’ at 6th year, in general. The net return is found to be highest between the age group of 4 to 6 years, 6thyear age garden giving the optimum yield and return. Attack of pest and disease is the major problem expressed by majority of the farmers along with other problems. Proper and timely technical guidance & suitable government initiative for development of ‘fore-warning system’, as well as establishment of newer plantation need to be encouraged with feasible assistance from the concerned line department(s).
大豆蔻(Amomum subulatum),当地人称其为 "alaichi",通常被称为香料之王。印度是这种古老香料的第二大生产国,占全球产量的 37%。本研究试图借助 2019-2020 年期间从西孟加拉邦卡林邦地区随机抽取的 60 位样本农民处收集的原始数据,分析大豆蔻种植的财务和经济表现。大豆蔻是研究地区最重要的经济作物之一。加勒特排序法用于确定大豆蔻生产过程中面临的主要制约因素。采用贴现法进行财务可行性分析后发现,在 7% 的贴现率下,效益成本比 (BCR) 为 3.01,净现值 (NPV) 为 ₹1,99,929.92(每公顷每年),这证实了大豆蔻种植的财务可行性和盈利能力。研究发现,该作物的产量随种植年限呈上升趋势,一般在第 6 年达到 "最大值"。净收益率在 4-6 年树龄组之间最高,第 6 年树龄园的产量和收益率最佳。除其他问题外,病虫害也是大多数农户面临的主要问题。需要在相关职能部门的可行协助下,鼓励适当、及时的技术指导和适当的政府举措,以开发 "预警系统 "和建立新的种植园。
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引用次数: 0
Fruits Variability of Some Nigerian Date-Palm (Phoenix dactylifera L.) Accessions as Revealed by Yield Parameters 产量参数揭示的尼日利亚枣椰树(Phoenix dactylifera L.)一些品种的果实变异性
Pub Date : 2023-08-10 DOI: 10.20546/ijcmas.2023.1208.026
S. Yahaya, O. Falusi, O. A. Daudu, A. Abubakar, N. I. Mariam, M. Abdulkarim, R. H. Aliyu, C. I. Okoye, M. Yakubu
The aim of this study was to investigate the fruit physical traits of some date palm accessions conserved in the experimental gene pool of NIFOR Date palm research substation in dutse, Nigeria. A survey and exploration were undertaken to collect the fruits from the population of the Female date palm (Phoenix dactylifera L.) trees germplasm across the gene pools. The results showed that the accessions R5P24 and R9P2 had the highest number of fruit length (4.73cm) and (4.87 cm). The accession R7P1 recorded the highest and significantly superior values (11.85 g) in fruit weight, while accession R24P9 recorded the lowest fruit diameter. The accessions R6P20, R14P21, and R5P20 showed no significant differences. Significant differences were recorded among the studied accessions in seed weight, seed length and seed diameter respectively.
本研究的目的是调查尼日利亚杜特塞 NIFOR 枣椰研究分站实验基因库中保存的一些枣椰品种的果实物理性状。通过调查和探索,从基因库中的雌性枣椰树(Phoenix dactylifera L.)种质群中收集了果实。结果表明,R5P24 和 R9P2 的果实长度(4.73 厘米)和(4.87 厘米)最高。R7P1 的果实重量(11.85 克)最高且明显优于 R24P9,而 R24P9 的果实直径最低。R6P20、R14P21 和 R5P20 之间没有显著差异。所研究的品种在种子重量、种子长度和种子直径方面分别存在显著差异。
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引用次数: 0
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International Journal of Current Microbiology and Applied Sciences
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