Pub Date : 2023-08-10DOI: 10.20546/ijcmas.2023.1208.002
Subhabrata Goswami, Satyabrata Manna, Tapati Adak, Raktim Chakraborty, Gora Chand Ghosh, Ajit Pradhhan, Sumana Dolui, Ranita Maji, Somnath De
Food-borne illnesses are becoming more prevalent worldwide as a result of eating contaminated refrigerated foods. The present study was undertaken to investigate the microbial load of different refrigerated food and their response to antimicrobial agents. A total of five processed food samples were taken aseptically, stored in the refrigerator for three days, and examined for microbial loads every day. Spread plate technique and biochemical characterisation had been used for the microbial screening of diverse food samples. The findings indicated that there were numerous contaminating bacteria present, which can cause a variety of food-borne diseases. Samples were analysed for the presence of bacteria while antibiotic resistance patterns of bacterial isolates were determined using standard methods. According to these studies, consumer refrigerator management and hygiene are of extremely low standards, endangering their health. In order to ensure prompt food safety, the study's findings emphasise the significance of proper temperature management and thorough, regular cleaning of their refrigerators.
{"title":"Assessment of Microbial Isolation from Refrigerated Food and their Response to Antimicrobial Agents","authors":"Subhabrata Goswami, Satyabrata Manna, Tapati Adak, Raktim Chakraborty, Gora Chand Ghosh, Ajit Pradhhan, Sumana Dolui, Ranita Maji, Somnath De","doi":"10.20546/ijcmas.2023.1208.002","DOIUrl":"https://doi.org/10.20546/ijcmas.2023.1208.002","url":null,"abstract":"Food-borne illnesses are becoming more prevalent worldwide as a result of eating contaminated refrigerated foods. The present study was undertaken to investigate the microbial load of different refrigerated food and their response to antimicrobial agents. A total of five processed food samples were taken aseptically, stored in the refrigerator for three days, and examined for microbial loads every day. Spread plate technique and biochemical characterisation had been used for the microbial screening of diverse food samples. The findings indicated that there were numerous contaminating bacteria present, which can cause a variety of food-borne diseases. Samples were analysed for the presence of bacteria while antibiotic resistance patterns of bacterial isolates were determined using standard methods. According to these studies, consumer refrigerator management and hygiene are of extremely low standards, endangering their health. In order to ensure prompt food safety, the study's findings emphasise the significance of proper temperature management and thorough, regular cleaning of their refrigerators.","PeriodicalId":13777,"journal":{"name":"International Journal of Current Microbiology and Applied Sciences","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139351063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-10DOI: 10.20546/ijcmas.2023.1208.001
H. Hamada, Kohji Ishihara, K. Shimoda, A. Kuboki, Y. Kiriake, Ryusuke Hosoda
Memantine (memary) oligosaccharides (gluco-oligosaccharides) were synthesized by enzymatic glycosylation, using cyclodextrin glucanotransferase as a biocatalyst. Although memantine hardly crosses the blood–brain barrier (BBB) in the mouse brain, memantine oligosaccharides crossed the BBB of mouse brain and were incorporated into the mouse brain tissue. Our investigations indicated that memantine modified with oligosaccharides might have gained a BBB-crossing ability. Furthermore, during the Y-maze test using senescence-accelerated mouse prone 8, our study revealed that the time spent in the novel Y-maze arm by the memantine-oligosaccharides-treated mouse was longer than that spent in the novel arm by the memantine-treated-mouse. Therefore, this study established that since memantine oligosaccharides could penetrate the BBB of mouse brain and be incorporated into the mouse’s brain tissue, they could also enhance spatial learning.
{"title":"Enzymatic Synthesis of Memantine (Memary) Oligosaccharides (Gluco-oligosaccharides) and their application as anti-dementia drugs that cross the Blood-Brain Barrier (BBB)","authors":"H. Hamada, Kohji Ishihara, K. Shimoda, A. Kuboki, Y. Kiriake, Ryusuke Hosoda","doi":"10.20546/ijcmas.2023.1208.001","DOIUrl":"https://doi.org/10.20546/ijcmas.2023.1208.001","url":null,"abstract":"Memantine (memary) oligosaccharides (gluco-oligosaccharides) were synthesized by enzymatic glycosylation, using cyclodextrin glucanotransferase as a biocatalyst. Although memantine hardly crosses the blood–brain barrier (BBB) in the mouse brain, memantine oligosaccharides crossed the BBB of mouse brain and were incorporated into the mouse brain tissue. Our investigations indicated that memantine modified with oligosaccharides might have gained a BBB-crossing ability. Furthermore, during the Y-maze test using senescence-accelerated mouse prone 8, our study revealed that the time spent in the novel Y-maze arm by the memantine-oligosaccharides-treated mouse was longer than that spent in the novel arm by the memantine-treated-mouse. Therefore, this study established that since memantine oligosaccharides could penetrate the BBB of mouse brain and be incorporated into the mouse’s brain tissue, they could also enhance spatial learning.","PeriodicalId":13777,"journal":{"name":"International Journal of Current Microbiology and Applied Sciences","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139351125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-10DOI: 10.20546/ijcmas.2023.1208.012
B. O. Mallikarjuna, T. N. Devaraja
Red gram (Cajanus cajan) grown in an area of 2.51 lakh hectares with the production of 1.21 lakh tonnes and productivity was 482 kg/ha in India. The Cluster Frontline Demonstrations C(FLD’s) on BRG-5 variety of pigeon pea were conducted by Krishi Vigyan Kendra of Davanagere District, Karnataka State.BRG-5 variety was released by University of Agricultural Sciences, Bengaluru during 2014-15, which is resistant to wilt. Improved cultivation practices were demonstrated in farmer’s fields for three years. The results of the demonstration showed higher yield when compared to the local (traditional variety) where we followed improved production technology for red gram. The average increased per cent seed yield is 30.88 over the years from front line demonstrations compared to farmer practice plots. However, during the year 2018-19, the per cent increase in seed yield was 37.72. Average gross return and net return were ₹ 49695 and ₹ 25916 ha-1 respectively in demonstration plot (BRG-5) as against ₹ 37162 and ₹ 17110 ha-1 in farmer practice (BRG-2). The other parameters which include extension gap, technological gap and technology index were studied for assessment of technology adoption rate. The average extension gap, technology gap and technological index were 2.82 q/ha, 6 q/ha and 33.35 per cent, respectively. The studies clearly indicates positive impact of frontline demonstrations against the farmers practice towards increased productivity in red gram by following the production technology under rain fed farming of Davangere district of Karnataka state, India.
{"title":"Frontline Demonstration A Tool to Study Drought Tolerant and High Yielding Red Gram Variety for Davanagere District, India","authors":"B. O. Mallikarjuna, T. N. Devaraja","doi":"10.20546/ijcmas.2023.1208.012","DOIUrl":"https://doi.org/10.20546/ijcmas.2023.1208.012","url":null,"abstract":"Red gram (Cajanus cajan) grown in an area of 2.51 lakh hectares with the production of 1.21 lakh tonnes and productivity was 482 kg/ha in India. The Cluster Frontline Demonstrations C(FLD’s) on BRG-5 variety of pigeon pea were conducted by Krishi Vigyan Kendra of Davanagere District, Karnataka State.BRG-5 variety was released by University of Agricultural Sciences, Bengaluru during 2014-15, which is resistant to wilt. Improved cultivation practices were demonstrated in farmer’s fields for three years. The results of the demonstration showed higher yield when compared to the local (traditional variety) where we followed improved production technology for red gram. The average increased per cent seed yield is 30.88 over the years from front line demonstrations compared to farmer practice plots. However, during the year 2018-19, the per cent increase in seed yield was 37.72. Average gross return and net return were ₹ 49695 and ₹ 25916 ha-1 respectively in demonstration plot (BRG-5) as against ₹ 37162 and ₹ 17110 ha-1 in farmer practice (BRG-2). The other parameters which include extension gap, technological gap and technology index were studied for assessment of technology adoption rate. The average extension gap, technology gap and technological index were 2.82 q/ha, 6 q/ha and 33.35 per cent, respectively. The studies clearly indicates positive impact of frontline demonstrations against the farmers practice towards increased productivity in red gram by following the production technology under rain fed farming of Davangere district of Karnataka state, India.","PeriodicalId":13777,"journal":{"name":"International Journal of Current Microbiology and Applied Sciences","volume":"160 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139351156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present investigation was conducted to examine the 17 bread rice genotypes including one check to study the genetic parameters, correlation and genetic diversity. The experiment was carried out in Research Farm Genetics & Plant Breeding of AKS University, Satna, M.P., during kharif season, 2021-22 in Randomized Block Design (RBD) with three replications. Analysis of variance showed highly significant differences among 17 bread rice genotypes for 14 characters studied. The statistical analysis of variance showed significant differences among genotypes for all 14 traits, including days to 50% flowering, plant height, effective tillers per plant, flag leaf length, maturity, panicle length, kernel length and width, test weight, harvest index, biological yield per plant and grain yield per plant. The results showed that these genotypes of rice crops have short flowering period, tall plant height, many effective tillers per plant, long flag leaf, long panicle and high grain yield per plant. The coefficients of variation were low, indicating little variation within the genotypes for each trait. The study results suggest significant variations in the characteristics of different genotypes of rice crops and can be useful for crop improvement. The genotypic correlation coefficients were generally higher than the phenotypic ones. Traits related to reproductive growth, such as number of effective tillers per plant, number of seeds per panicle, and harvest index, had positive effects on grain yield per plant, while traits related to vegetative growth, such as days to 50% flowering and plant height, had negative effects. The path coefficient analysis indicated that biological yield per plant and harvest index had the maximum positive effect on grain yield per plant, while spikelet’s per spike, grain per spike, effective tiller per plant, days to maturity, and days to 50% flowering had the maximum negative effect. These findings provide valuable insights for rice crop improvement.
{"title":"Harnessing the Genetic Variability and Trait Association of Yield and Yield Related Traits in Rice (Oryza sativa L.)","authors":"Lavkesh Kumar Lodhi, Bineeta Singh, Shraddha Chowhan","doi":"10.20546/ijcmas.2023.1208.015","DOIUrl":"https://doi.org/10.20546/ijcmas.2023.1208.015","url":null,"abstract":"The present investigation was conducted to examine the 17 bread rice genotypes including one check to study the genetic parameters, correlation and genetic diversity. The experiment was carried out in Research Farm Genetics & Plant Breeding of AKS University, Satna, M.P., during kharif season, 2021-22 in Randomized Block Design (RBD) with three replications. Analysis of variance showed highly significant differences among 17 bread rice genotypes for 14 characters studied. The statistical analysis of variance showed significant differences among genotypes for all 14 traits, including days to 50% flowering, plant height, effective tillers per plant, flag leaf length, maturity, panicle length, kernel length and width, test weight, harvest index, biological yield per plant and grain yield per plant. The results showed that these genotypes of rice crops have short flowering period, tall plant height, many effective tillers per plant, long flag leaf, long panicle and high grain yield per plant. The coefficients of variation were low, indicating little variation within the genotypes for each trait. The study results suggest significant variations in the characteristics of different genotypes of rice crops and can be useful for crop improvement. The genotypic correlation coefficients were generally higher than the phenotypic ones. Traits related to reproductive growth, such as number of effective tillers per plant, number of seeds per panicle, and harvest index, had positive effects on grain yield per plant, while traits related to vegetative growth, such as days to 50% flowering and plant height, had negative effects. The path coefficient analysis indicated that biological yield per plant and harvest index had the maximum positive effect on grain yield per plant, while spikelet’s per spike, grain per spike, effective tiller per plant, days to maturity, and days to 50% flowering had the maximum negative effect. These findings provide valuable insights for rice crop improvement.","PeriodicalId":13777,"journal":{"name":"International Journal of Current Microbiology and Applied Sciences","volume":"61 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139351078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-10DOI: 10.20546/ijcmas.2023.1208.025
Samiksha Bisht, Anju K Dhiman, S. Attri, Deepika Kathuria
Persimmon is also known as Japaniphal (Diospyros kaki L.) used for preparing dehydrated persimmon slices. Among different pre-treatments (blanching, potassium metabisulphite, citric acid, ascorbic acid) used prior to drying, the steam blanching for 3 min + KMS (300 ppm) + citric acid (0.3 %) immersed for 20 minutes in water found to be highest sensory scores therefore considered best. The pretreated slices of persimmon were further dried using different drying techniques sun (T1), solar (T2) and mechanical cabinet (T3). The best drying method was mechanical cabinet (T3) based on highest value of chemical characteristics and greatest scores for sensory. When stored for six months, the decrease in constituents like ascorbic acid and β-carotene (8.96 to 8.66 and 17.92 to 13.47 mg/100 g), fibre content (1.25 to 1.04 %), antioxidant activity (63.19 to 59.59 %) and total phenols (14.05 to 10.30 mg/100 g) was minimum in slices dried in dehydrator (mechanical cabinet) in contrast with dryer (solar tunnel and solar glass). The sensory scores showed acceptable limit but remained decreased during storage. The minimal changes in both (chemical and sensory) attributes generally with the result ensured that dried persimmon slices prepared surely be stored safely for the duration of 6 months.
{"title":"Changes on Nutritional Quality of Ripe Persimmon When Pre-Treated with Different Pre-Treatments and Drying Methods","authors":"Samiksha Bisht, Anju K Dhiman, S. Attri, Deepika Kathuria","doi":"10.20546/ijcmas.2023.1208.025","DOIUrl":"https://doi.org/10.20546/ijcmas.2023.1208.025","url":null,"abstract":"Persimmon is also known as Japaniphal (Diospyros kaki L.) used for preparing dehydrated persimmon slices. Among different pre-treatments (blanching, potassium metabisulphite, citric acid, ascorbic acid) used prior to drying, the steam blanching for 3 min + KMS (300 ppm) + citric acid (0.3 %) immersed for 20 minutes in water found to be highest sensory scores therefore considered best. The pretreated slices of persimmon were further dried using different drying techniques sun (T1), solar (T2) and mechanical cabinet (T3). The best drying method was mechanical cabinet (T3) based on highest value of chemical characteristics and greatest scores for sensory. When stored for six months, the decrease in constituents like ascorbic acid and β-carotene (8.96 to 8.66 and 17.92 to 13.47 mg/100 g), fibre content (1.25 to 1.04 %), antioxidant activity (63.19 to 59.59 %) and total phenols (14.05 to 10.30 mg/100 g) was minimum in slices dried in dehydrator (mechanical cabinet) in contrast with dryer (solar tunnel and solar glass). The sensory scores showed acceptable limit but remained decreased during storage. The minimal changes in both (chemical and sensory) attributes generally with the result ensured that dried persimmon slices prepared surely be stored safely for the duration of 6 months.","PeriodicalId":13777,"journal":{"name":"International Journal of Current Microbiology and Applied Sciences","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139351200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-10DOI: 10.20546/ijcmas.2023.1208.031
G. B. Srinivasulu, M. Shiragur, A. Shirol, N. Basavaraja, P. Gangadharappa, Pavan kumar
The experiment was conducted at College of Horticulture, Munirabad, Koppal district Karnataka with an object to study the post harvest studies in Crossandra which was carried out under lab condition during the year 2020-21 comprising of nine treatments with three replications and design adopted was CRD. The details of chemical treatments includes T1–Sucrose – 2 %, T2–Sucrose – 4 %, T3- Boric acid – 2 %, T4- Boric acid – 4 %, T5– Aluminium sulphate - 2 %, T6-Aluminium sulphate – 4 %, T7– Ascorbic acid 100 ppm, T8- Ascorbic acid 200 ppm, T9- Control. The results indicated that spraying of ascorbic acid @ 200 ppm directly to flowers enhanced shelf life of Crossandra.
{"title":"Influence of Post-harvest Chemical Treatments on Shelf Life of Crossandra","authors":"G. B. Srinivasulu, M. Shiragur, A. Shirol, N. Basavaraja, P. Gangadharappa, Pavan kumar","doi":"10.20546/ijcmas.2023.1208.031","DOIUrl":"https://doi.org/10.20546/ijcmas.2023.1208.031","url":null,"abstract":"The experiment was conducted at College of Horticulture, Munirabad, Koppal district Karnataka with an object to study the post harvest studies in Crossandra which was carried out under lab condition during the year 2020-21 comprising of nine treatments with three replications and design adopted was CRD. The details of chemical treatments includes T1–Sucrose – 2 %, T2–Sucrose – 4 %, T3- Boric acid – 2 %, T4- Boric acid – 4 %, T5– Aluminium sulphate - 2 %, T6-Aluminium sulphate – 4 %, T7– Ascorbic acid 100 ppm, T8- Ascorbic acid 200 ppm, T9- Control. The results indicated that spraying of ascorbic acid @ 200 ppm directly to flowers enhanced shelf life of Crossandra.","PeriodicalId":13777,"journal":{"name":"International Journal of Current Microbiology and Applied Sciences","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139351140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-10DOI: 10.20546/ijcmas.2023.1208.023
S. Raveesha, P. Pushpa, Rajashekar D. Barker
India is the world’s largest producer of pomegranate followed by Iran, Turkey, Spain, China, Morocco, Afghanistan, Japan, Greece, France, Armenia, Egypt, Italy and Palestine. Karnataka stands second largest pomegranate producing state in the country with an area of 28,090ha, production of about 3,28,920 tonnes and productivity of 11.71 MT per hectare. The major producing districts are Chitradurga, Vijayapura, Tumkuru, Dharwad, Bagalkote and Koppal (Horticulture statistics at a glance, 2017). The present study was undertaken in Chitradurga district, where area under pomegranate was significant. Among the six taluks in Chitradurga district, three taluks Viz., Hiriyur, Hosadurga and Challakere were selected for the study based on the highest acreage under the crop. Primary data was collected from the 90 sample respondents in the study area and the secondary data related to district wise area, production and productivity was collected from District Horticulture office. Tabular analysis was adopted to compile the cropping pattern of the sample farmers, Simple statistical tools like averages and percentages were used to interpret results properly. The data obtained on area, production and productivity of pomegranate. The results indicated that there was positive growth under pomegranate area at state level (29.64%) and in the Chitradurga district (33.20%). With respect to production also a positive trend of 32.95 per cent in the state level and 34.93 per cent in Chitradurga. The positive growth in production observed was due to increase in area under pomegranate cultivation. Productivity growth was also shown positive but at an increased growth rate of 2.55 per cent in Karnataka state and 3.54 per cent in Chitradurga district.
{"title":"Cropping Pattern, Growth Rate in Area, Production and Productivity of Pomegranate Cultivation in Chitradurga District of Karnataka, India","authors":"S. Raveesha, P. Pushpa, Rajashekar D. Barker","doi":"10.20546/ijcmas.2023.1208.023","DOIUrl":"https://doi.org/10.20546/ijcmas.2023.1208.023","url":null,"abstract":"India is the world’s largest producer of pomegranate followed by Iran, Turkey, Spain, China, Morocco, Afghanistan, Japan, Greece, France, Armenia, Egypt, Italy and Palestine. Karnataka stands second largest pomegranate producing state in the country with an area of 28,090ha, production of about 3,28,920 tonnes and productivity of 11.71 MT per hectare. The major producing districts are Chitradurga, Vijayapura, Tumkuru, Dharwad, Bagalkote and Koppal (Horticulture statistics at a glance, 2017). The present study was undertaken in Chitradurga district, where area under pomegranate was significant. Among the six taluks in Chitradurga district, three taluks Viz., Hiriyur, Hosadurga and Challakere were selected for the study based on the highest acreage under the crop. Primary data was collected from the 90 sample respondents in the study area and the secondary data related to district wise area, production and productivity was collected from District Horticulture office. Tabular analysis was adopted to compile the cropping pattern of the sample farmers, Simple statistical tools like averages and percentages were used to interpret results properly. The data obtained on area, production and productivity of pomegranate. The results indicated that there was positive growth under pomegranate area at state level (29.64%) and in the Chitradurga district (33.20%). With respect to production also a positive trend of 32.95 per cent in the state level and 34.93 per cent in Chitradurga. The positive growth in production observed was due to increase in area under pomegranate cultivation. Productivity growth was also shown positive but at an increased growth rate of 2.55 per cent in Karnataka state and 3.54 per cent in Chitradurga district.","PeriodicalId":13777,"journal":{"name":"International Journal of Current Microbiology and Applied Sciences","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139351157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-10DOI: 10.20546/ijcmas.2023.1208.028
M. K. Krishnaveni, A. Bilolikar, Sister Mariyamma Thomas
The transmission of nosocomial pathogens in a hospital is a serious and growing concern worldwide. Environmental reservoirs could be playing a larger role in transmission than was first realized. This study evaluated environmental surveillance for nosocomial pathogens isolated from patients with hospital acquired infections. All the samples collected from the patients and environment were inoculated onto Mac Conkey Agar and 5% Sheep Blood Agar. These culture media were incubated overnight for 37°C. The growths obtained on the culture media were further processed for identification and antimicrobial susceptibility testing by Vitek 2 Compact according to manufacturer’s instructions. A cluster of neurosurgical site infections post craniotomy isolated Serratia marcescens, which was associated with shaving razors contaminated by S. marcescens. In the medical ICU, 2 patients placed next to each other isolated Burkholderia cepacia in their blood cultures. Burkholderia cepacia was isolated from the rim of the sink in the handwash station. Following cardiac surgeries of two patients, both patients were infected with Pseudomonas aeruginosa at the surgical site. The same isolate was isolated from operation theatre overhead light handle and door handle of cardiothoracic ICU. In the paediatric ICU, Acinetobacter baumanii was isolated from the endotracheal secretions of two patients. Culture of water and environmental samples grew Acinetobacter baumanii from the oxygen vacuum jar. The environment of a hospital can contribute to hospital acquired infections. Hence, a diligent housekeeping protocol would reduce the risk of transmission of nosocomial pathogens from the environment.
{"title":"Potential Reservoirs of Nosocomial Pathogens in a Hospital: An Investigational Study","authors":"M. K. Krishnaveni, A. Bilolikar, Sister Mariyamma Thomas","doi":"10.20546/ijcmas.2023.1208.028","DOIUrl":"https://doi.org/10.20546/ijcmas.2023.1208.028","url":null,"abstract":"The transmission of nosocomial pathogens in a hospital is a serious and growing concern worldwide. Environmental reservoirs could be playing a larger role in transmission than was first realized. This study evaluated environmental surveillance for nosocomial pathogens isolated from patients with hospital acquired infections. All the samples collected from the patients and environment were inoculated onto Mac Conkey Agar and 5% Sheep Blood Agar. These culture media were incubated overnight for 37°C. The growths obtained on the culture media were further processed for identification and antimicrobial susceptibility testing by Vitek 2 Compact according to manufacturer’s instructions. A cluster of neurosurgical site infections post craniotomy isolated Serratia marcescens, which was associated with shaving razors contaminated by S. marcescens. In the medical ICU, 2 patients placed next to each other isolated Burkholderia cepacia in their blood cultures. Burkholderia cepacia was isolated from the rim of the sink in the handwash station. Following cardiac surgeries of two patients, both patients were infected with Pseudomonas aeruginosa at the surgical site. The same isolate was isolated from operation theatre overhead light handle and door handle of cardiothoracic ICU. In the paediatric ICU, Acinetobacter baumanii was isolated from the endotracheal secretions of two patients. Culture of water and environmental samples grew Acinetobacter baumanii from the oxygen vacuum jar. The environment of a hospital can contribute to hospital acquired infections. Hence, a diligent housekeeping protocol would reduce the risk of transmission of nosocomial pathogens from the environment.","PeriodicalId":13777,"journal":{"name":"International Journal of Current Microbiology and Applied Sciences","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139351206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-10DOI: 10.20546/ijcmas.2023.1208.014
Mangalagiri Swetcha, S. Sureshkhannan, K. Porteen, S. Ezhilvelan
Pseudomonas aeruginosa is an opportunistic pathogen and is ubiquitous in nature and commonly found in soil, water, plants, and raw meat. It is major predominant spoilage bacteria in meat under refrigerated conditions. The study aimed to characterize the isolation, identification and molecular confirmation of Pseudomonas aeruginosa in Chicken meat, Mutton and Pork. Study area, sampling, isolation, identification and molecular confirmation of Pseudomonas aeruginosa. A total 210 (n = 35 from each of the species) aerobically stored and frozen meat samples were collected from retail markets and slaughter houses in and around Chennai, India and analyzed. Among them, 22 isolates were confirmed as Pseudomonas aeruginosa by conventional, culture, biochemical and PCR assay targeting 16 SrRNA sequencing and amplification of rpoBgene. In this research, we successfully studied the occurrence, isolation, identification and molecular confirmation of Pseudomonas aeruginosa isolated from Chicken meat, Mutton and pork using conventional techniques.
{"title":"Isolation and Molecular Confirmation of Pseudomonas aeruginosa in Chicken meat, Mutton and Pork","authors":"Mangalagiri Swetcha, S. Sureshkhannan, K. Porteen, S. Ezhilvelan","doi":"10.20546/ijcmas.2023.1208.014","DOIUrl":"https://doi.org/10.20546/ijcmas.2023.1208.014","url":null,"abstract":"Pseudomonas aeruginosa is an opportunistic pathogen and is ubiquitous in nature and commonly found in soil, water, plants, and raw meat. It is major predominant spoilage bacteria in meat under refrigerated conditions. The study aimed to characterize the isolation, identification and molecular confirmation of Pseudomonas aeruginosa in Chicken meat, Mutton and Pork. Study area, sampling, isolation, identification and molecular confirmation of Pseudomonas aeruginosa. A total 210 (n = 35 from each of the species) aerobically stored and frozen meat samples were collected from retail markets and slaughter houses in and around Chennai, India and analyzed. Among them, 22 isolates were confirmed as Pseudomonas aeruginosa by conventional, culture, biochemical and PCR assay targeting 16 SrRNA sequencing and amplification of rpoBgene. In this research, we successfully studied the occurrence, isolation, identification and molecular confirmation of Pseudomonas aeruginosa isolated from Chicken meat, Mutton and pork using conventional techniques.","PeriodicalId":13777,"journal":{"name":"International Journal of Current Microbiology and Applied Sciences","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139351110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A total 12 samples of yak dung, compost and soil were collected and processed microbiologically for cellulose decomposer ability and identification of efficient isolates from high altitude from Lahaul-Spiti and Kinnaur districts. Based on the colony morphology, 34 bacterial isolates (08 from yak dung, 19 from soil and 07 from manure samples) recovered on nutrient agar. 24 isolates were of cellulose degrading nature further identified biochemically and 6 isolates were of Bacillus spp. And 01 isolate was of Pseudomonas spp. These isolates were employed for biological pretreatment of paddy straw for two weeks and then run in laboratory biogas digester along with control digester. The digester set was observed from the second day of run upto three weeks and it was observed that the there was high biogas production in test digester as compared to control digester. It was concluded from out study that the utilization of consortium of Bacillus spp and Pseudomonas spp found 10 percent higher biogas production at ambient temperature from rice straw.
{"title":"Effect of Biological Pre-Treatment of Agricultural Waste on Biogas Production","authors":"Rajinder Kumar, Dinesh Kumar Vatsa, Gurpreet Singh, Kanika Baghla","doi":"10.20546/ijcmas.2023.1208.008","DOIUrl":"https://doi.org/10.20546/ijcmas.2023.1208.008","url":null,"abstract":"A total 12 samples of yak dung, compost and soil were collected and processed microbiologically for cellulose decomposer ability and identification of efficient isolates from high altitude from Lahaul-Spiti and Kinnaur districts. Based on the colony morphology, 34 bacterial isolates (08 from yak dung, 19 from soil and 07 from manure samples) recovered on nutrient agar. 24 isolates were of cellulose degrading nature further identified biochemically and 6 isolates were of Bacillus spp. And 01 isolate was of Pseudomonas spp. These isolates were employed for biological pretreatment of paddy straw for two weeks and then run in laboratory biogas digester along with control digester. The digester set was observed from the second day of run upto three weeks and it was observed that the there was high biogas production in test digester as compared to control digester. It was concluded from out study that the utilization of consortium of Bacillus spp and Pseudomonas spp found 10 percent higher biogas production at ambient temperature from rice straw.","PeriodicalId":13777,"journal":{"name":"International Journal of Current Microbiology and Applied Sciences","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139351127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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