International dental journal最新文献

{"title":"The Role of Interferon-γ-Induced Granzyme B in Enhancing Antitumour Immune Responses in Oral Squamous Cell Carcinoma","authors":"Hongrong Zhang ,&nbsp;Xue Zhou ,&nbsp;Lei Feng ,&nbsp;Jingyi Li ,&nbsp;Wanyue Zhang ,&nbsp;Zheyi Sun ,&nbsp;Weihong Wang","doi":"10.1016/j.identj.2025.109377","DOIUrl":"10.1016/j.identj.2025.109377","url":null,"abstract":"<div><h3>Background</h3><div>Oral squamous cell carcinoma (OSCC) is associated with poor clinical outcomes and the immune status of its tumour microenvironment (TME) plays a critical role in shaping prognosis. Transcriptomic profiling has become a powerful tool for dissecting immune heterogeneity and identifying key regulatory molecules in OSCC. As central mediators of antigen presentation and antitumor immune activation, major histocompatibility complex (MHC) molecules are essential components of the immune response; however, the prognostic value and regulatory mechanisms of MHC-related genes in OSCC remain insufficiently defined.</div></div><div><h3>Methods</h3><div>OSCC transcriptomic data from TCGA and GEO were integrated to construct a prognostic RiskScore using differential expression and Cox analysis. Immune infiltration was assessed with ESTIMATE. Immunohistochemistry and immunofluorescence were performed on clinical OSCC tissues to evaluate the relationship between granzyme B (GZMB) and CD8⁺ T cells. A C3H tumour-bearing mouse model with intratumoral IFN-γ injection was used to validate the effects of IFN-γ on tumour growth, immune infiltration and GZMB expression.</div></div><div><h3>Results</h3><div>An eight-gene MHC-related RiskScore was identified as an independent prognostic factor for OSCC. GZMB was highlighted as a key gene, highly expressed in early-stage OSCC and strongly correlated with activated CD8⁺ T cells, but reduced in advanced disease. IFN-γ administration in vivo inhibited tumour growth, increased Cd3⁺ T-cell infiltration and enhanced IFN-γ and GZMB co-expression within necrotic regions.</div></div><div><h3>Conclusion</h3><div>This study established a robust prognostic model for OSCC based on MHC-related genes and demonstrated that IFN-γ enhances antitumor immunity through GZMB-mediated cytotoxic activation. The IFN-γ–GZMB axis appears to represent a critical regulatory pathway within the OSCC immune microenvironment and provide a promising therapeutic target and mechanistic basis for immunotherapy in OSCC</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"76 2","pages":"Article 109377"},"PeriodicalIF":3.7,"publicationDate":"2026-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single vs Triple-Headed Toothbrushes in Children With Special Needs: A Randomised Crossover Trial of Brushing Efficacy","authors":"Thanapon Tanaiadchawoot ,&nbsp;Apiwan Smutkeeree ,&nbsp;Sudaporn Khamduangdao ,&nbsp;Pornpailin Kasemkhun","doi":"10.1016/j.identj.2025.109388","DOIUrl":"10.1016/j.identj.2025.109388","url":null,"abstract":"<div><h3>Introduction and aims</h3><div>Children with special needs (CSN) often struggle with effective toothbrushing, which is attributed to factors such as toothbrush type and visual-motor integration (VMI). This study aimed to compare the brushing efficacy of single-headed toothbrushes (SHT) vs triple-headed toothbrushes (THT) at varying VMI levels.</div></div><div><h3>Methods</h3><div>This single-blind, randomised controlled crossover study involved 46 CSN ranging in age from 6.1 to 12.0 years. Baseline plaque index (PI), gingival index (GI), and VMI levels were measured. Participants were assigned a toothbrush type using block randomisation and received oral hygiene instructions accordingly. After participants completed self-brushing, immediate post-brushing PI, brushing durations, and brushing performance were assessed. Following a 1-month washout period, the process was repeated using the alternate toothbrush type. A t-test statistical analysis of 2 × 2 crossover design was utilised.</div></div><div><h3>Results</h3><div>THT use resulted in significantly lower post-brushing PI and a greater percentage of plaque reduction (<em>P</em> &lt; .001). Within similar VMI levels, THT showed significantly better plaque removal than SHT in both normal (<em>P</em> = .02) and impaired (<em>P</em> = .009) VMI groups. However, there was no statistically significant difference between VMI levels. Additionally, THT required less brushing time (2.3 ± 0.9 vs 3.3 ± 0.9 minutes, <em>P</em> &lt; .001) and provided superior cleaning on lingual surfaces across all tooth areas (<em>P</em> &lt; .001-.041), except for the first permanent molars.</div></div><div><h3>Conclusion</h3><div>THT demonstrated superior plaque removal and reduced brushing time in CSN, regardless of VMI level. It also enhanced cleaning of difficult-to-reach lingual/palatal surfaces, although its efficacy was comparable to SHT in the first permanent molar areas.</div></div><div><h3>Clinical relevance</h3><div>For CSN who brush independently, the use of THT may improve oral hygiene and reduce the burden on caregivers. Dental professionals should consider recommending THT for this cohort.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"76 2","pages":"Article 109388"},"PeriodicalIF":3.7,"publicationDate":"2026-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146035000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Antimicrobial Effect of Tetrahydrocannabinol on Streptococcus mutans and Its Anticariogenic Potential","authors":"Haoyan Zhai ,&nbsp;Yixuan Zhang ,&nbsp;Minki Kim ,&nbsp;Xintian Zhou ,&nbsp;Joseph Ferracciolo ,&nbsp;Eric Krukonis ,&nbsp;Chunyan Liu ,&nbsp;Zheng Zhou","doi":"10.1016/j.identj.2025.109386","DOIUrl":"10.1016/j.identj.2025.109386","url":null,"abstract":"<div><h3>Introduction and aims</h3><div>With the increasing use of marijuana, it is vital to understand the effect of tetrahydrocannabinol (THC) on oral microbiota, especially the primary carious pathogen <em>Streptococcus mutans</em>.</div></div><div><h3>Methods</h3><div>The minimum inhibitory concentration (MIC) of THC against <em>S mutans</em> was determined by antimicrobial susceptibility testing. Bacterial acid production was evaluated. The effect of THC on <em>S mutans</em> biofilm formation and preformed biofilms was determined by crystal violet assay. The metabolic activity and viability of the biofilm were assessed using the methylthiazolyldiphenyl tetrazolium bromide assay and live/dead assay, respectively. Extracellular polysaccharide (EPS) was examined by Cascade Blue Dextran staining. <em>S mutans</em> membrane potential was detected by the Baclight Bacterial Membrane Potential Kit.</div></div><div><h3>Results</h3><div>The MIC of THC against <em>S mutans</em> was 2 µg/mL (<em>P</em> &lt; .0001). A total of ≥2 µg/mL THC reduced bacterial acidogenicity and inhibited over 90% of biofilm formation (<em>P</em> &lt; .0001). Additionally, ≥1 µg/mL THC reduced biofilm viability and EPS production (<em>P</em> &lt; .0001), as assessed by fluorescence measurements and microscopy. While 1 to 64 µg/mL THC did not degrade preformed biofilm, metabolic activity was reduced by 16 to 64 µg/mL THC (<em>P</em> &lt; .01), and 8 to 32 µg/mL THC reduced biofilm viability in a time- and dose-dependent manner (<em>P</em> &lt; .001). Moreover, 2 to 8 µg/mL THC promoted membrane hyperpolarization after a 5-minute treatment (<em>P</em> &lt; .01).</div></div><div><h3>Conclusion</h3><div>THC inhibits <em>S mutans</em> growth and biofilm formation while also reducing bacterial viability, EPS production, and acid production. Although it does not degrade preformed biofilm biomass, THC diminishes its metabolic activity and viability. These effects may be linked to THC-induced membrane hyperpolarization. This in vitro study suggests that THC may reduce the cariogenic capacity of <em>S mutans.</em></div></div><div><h3>Clinical Relevance</h3><div>This study shows that THC inhibits <em>S mutans</em> growth, biofilm formation, properties of preformed biofilms, and acid production. It provides preliminary scientific evidence on the impact of THC on oral health, specifically cannabinoid consumption on cariogenesis, and a potential new avenue for developing a new anticariogenic agent.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"76 2","pages":"Article 109386"},"PeriodicalIF":3.7,"publicationDate":"2026-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146035002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to ‘Quality and Readability Assessment of Patient-Centered Online Information on Apical Surgery’ [International Dental Journal Volume 75, Issue 5, October 2025, 100955]","authors":"Pablo Álvarez-Nóvoa ,&nbsp;Ramón Miguéns-Vila ,&nbsp;Benjamín Martín-Biedma ,&nbsp;Gustavo De-Deus ,&nbsp;María Pilar Pecci-Lloret ,&nbsp;Miriam Teulé-Trull ,&nbsp;Juan Seoane-Romero ,&nbsp;Pablo Castelo-Baz","doi":"10.1016/j.identj.2025.109385","DOIUrl":"10.1016/j.identj.2025.109385","url":null,"abstract":"","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"76 2","pages":"Article 109385"},"PeriodicalIF":3.7,"publicationDate":"2026-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is Online Oriented-Centred Information About Dry Mouth Trustworthy? An Infodemiologic Study","authors":"Muath Alassaf ,&nbsp;Shadan Sharbib ,&nbsp;Reem Alhammad ,&nbsp;Hattan Zaki ,&nbsp;Hamzah Babkair ,&nbsp;Ahmad Othman ,&nbsp;Albraa Alolayan ,&nbsp;Shadia Elsayed","doi":"10.1016/j.identj.2025.109355","DOIUrl":"10.1016/j.identj.2025.109355","url":null,"abstract":"<div><h3>Introduction and aims</h3><div>Xerostomia is a common condition with significant effects on oral health and quality of life. Patients frequently seek health information online; however, the quality and reliability of such information remain questionable. This study aimed to evaluate the quality, reliability, and readability of patient-oriented information about xerostomia available on different websites.</div></div><div><h3>Methods</h3><div>A systematic search was conducted using Google, Bing, and Yahoo with the terms ‘dry mouth’ and ‘xerostomia’. Eligible websites were evaluated for source type and assessed using the Journal of the American Medical Association benchmarks, Health on the Net criteria, and the DISCERN instrument. Readability was measured using the Simple Measure of Gobbledygook and Flesch Reading Ease Score.</div></div><div><h3>Results</h3><div>A total of 203 websites were analysed. Nonprofit organizations were the most frequent source (45.8%), followed by dental/medical centres (36.9%), commercial sites (12.3%), and governmental/university sources (4.9%). The median DISCERN score indicated medium quality (3.5). Readability was high across all sites (median Flesch Reading Ease Score: 94.75; Simple Measure of Gobbledygook: 3.2), with no significant differences except in word and sentence counts. Nonprofit websites scored the highest for reliability and quality according to DISCERN and Journal of the American Medical Association criteria.</div></div><div><h3>Conclusion</h3><div>Online information about xerostomia is generally highly readable but varies in quality and reliability. Nonprofit websites provided the most trustworthy and comprehensive resources, while governmental and university-affiliated websites were underrepresented.</div></div><div><h3>Clinical relevance</h3><div>Given the variety in the quality and readability of online material about xerostomia, enhancing the reliability of web-based health resources necessitates a collaborative effort by healthcare providers. Encouraging website developers and organizations to use standardized evaluation tools to assure the accuracy and accessibility of online content can improve patient education and help informed decision-making and enhance digital health literacy.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"76 2","pages":"Article 109355"},"PeriodicalIF":3.7,"publicationDate":"2026-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146029576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"4632427E13Rik Facilitates Jaw Marrow-Derived Mesenchymal Stem Cells Osteogenesis and Angiogenesis Under Hypoxia Through miR-34a-5p/Aldoa/Hif-1α Pathway","authors":"Zhenning Wang ,&nbsp;Yu Zhang ,&nbsp;Yan Wang ,&nbsp;Rui Xue ,&nbsp;Qiaozhen Qin ,&nbsp;Heyang Zhang ,&nbsp;Zhenhua Xu ,&nbsp;Shuirong Liu ,&nbsp;Liu Liu ,&nbsp;Xiaoxia Jiang ,&nbsp;Lulu Xu","doi":"10.1016/j.identj.2025.109364","DOIUrl":"10.1016/j.identj.2025.109364","url":null,"abstract":"<div><h3>Introduction and aims</h3><div>The regeneration and repair of maxillofacial bone defects remain a significant clinical challenge. Jaw marrow-derived mesenchymal stem cells (JMMSCs) have attracted considerable attention owing to their favourable biological properties; however, the low survival rate of transplanted MSCs in vivo has substantially limited their therapeutic application. In this study, we demonstrate that hypoxic preconditioning enhances both the osteogenic and angiogenic potential of JMMSCs in vitro and in vivo.</div></div><div><h3>Methods</h3><div>In vitro, the expression levels of osteogenic and angiogenic-related genes were assessed via qRT-PCR and Western blot following hypoxic preconditioning. In vivo, the impact of hypoxic preconditioning on the bone defect repair capacity of JMMSCs was evaluated. Micro–CT scanning was employed to compare microstructural changes at the defect sites. Microarray analysis identified differentially expressed long noncoding RNAs (lncRNAs) in JMMSCs subjected to hypoxic preconditioning. Subsequent bioinformatics analysis was conducted to explore potential regulatory interactions and key signalling pathways among lncRNAs, miRNAs, and mRNAs, followed by experimental validation.</div></div><div><h3>Results</h3><div>Microarray analysis revealed a significant upregulation of lnc-4632427E13Rik in JMMSCs following hypoxic preconditioning. Mechanistic studies demonstrated that lnc-4632427E13Rik functions as a molecular ‘sponge’ by sequestering miR-34a-5p, leading to activation of the Aldoa/ERK/Hif-1α signalling axis and subsequent promotion of both angiogenesis and osteogenesis in JMMSCs.</div></div><div><h3>Conclusion</h3><div>Our findings reveal hypoxic preconditioning as a highly effective strategy for enhancing the differentiation potential of JMMSCs, and further highlight the therapeutic potential of targeting the lnc-4632427E13Rik/miR-34a-5p/Aldoa axis to improve the bone repair capacity of JMMSCs.</div></div><div><h3>Clinical relevance</h3><div>The lnc-4632427E13Rik/miR-34a-5p/Aldoa axis significantly enhances the efficiency and reliability of bone regeneration, representing a promising therapeutic target for clinical applications in the repair of maxillofacial bone defects.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"76 2","pages":"Article 109364"},"PeriodicalIF":3.7,"publicationDate":"2026-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146018470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SERPINA1 as a Shared Biomarker in Periodontitis and Oral Squamous Cell Carcinoma","authors":"Jingkun Zhang ,&nbsp;Guangwei Chen ,&nbsp;Qi Wang ,&nbsp;Yali Chi ,&nbsp;Guoxu Han","doi":"10.1016/j.identj.2025.109378","DOIUrl":"10.1016/j.identj.2025.109378","url":null,"abstract":"<div><h3>Objective</h3><div>Periodontitis (PD) and oral squamous cell carcinoma (OSCC) frequently co‑occur in clinical populations. However, shared molecular determinants that could support risk assessment across diseases remain insufficiently defined. This study aimed to identify shared diagnostic biomarkers and potential mechanisms linking PD and OSCC using integrated bioinformatics and machine learning.</div></div><div><h3>Methods</h3><div>PD and OSCC transcriptomic datasets were analysed to identify shared programmed cell death-related biomarkers using differentially expressed genes (DEGs), WGCNA and multiple machine learning algorithms (LASSO, Random Forest, GLM). Diagnostic performance was validated in external cohorts via ROC analysis, while immune landscapes and cellular interactions were characterised using CIBERSORT and single-cell analysis. At the cellular level, we evaluated SERPINA1 expression in an LPS-induced periodontal inflammation model and conducted siRNA-mediated knockdown in CAL27 oral cancer cells to examine its effects on proliferation, migration and invasion.</div></div><div><h3>Results</h3><div>Transcriptomic analyses of PD and OSCC revealed a shared immune-inflammatory signature with enrichment of programmed cell death pathways. SERPINA1 was consistently prioritised by LASSO, random forest and GLM and showed strong diagnostic performance across training and external validation cohorts (AUC &gt; 0.8). CIBERSORT indicated remodelling of the immune microenvironment associated with SERPINA1 expression, with positive correlations to macrophage and neutrophil abundance. Single-cell analyses localised SERPINA1 to myeloid populations and suggested putative crosstalk with the pro-inflammatory mediator interleukin-1β (IL-1β). In the periodontal inflammation model, SERPINA1 expression was significantly upregulated upon LPS stimulation alongside increased inflammatory mediators such as IL-8 and IL-1β. In contrast, SERPINA1 knockdown in oral cancer cells led to reduced proliferative, migratory and invasive capacities.</div></div><div><h3>Conclusions</h3><div>SERPINA1 emerges as a cross‑disease candidate biomarker linking chronic periodontal inflammation and oral cancer biology. The integrative framework provides a transparent roadmap for identifying clinically meaningful, reproducible biomarkers across oral diseases, going forwards.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"76 2","pages":"Article 109378"},"PeriodicalIF":3.7,"publicationDate":"2026-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146010200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy Assessment of a Novel Device for Dental Aerosol Capture and Inactivation: An In Vitro Study","authors":"Yan Sun ,&nbsp;Jia-Chun Li ,&nbsp;Yu-Gang Li ,&nbsp;Xiao-Yan Yu ,&nbsp;Jie Hu ,&nbsp;Zhe Li ,&nbsp;Ji-Chao Cao","doi":"10.1016/j.identj.2025.109331","DOIUrl":"10.1016/j.identj.2025.109331","url":null,"abstract":"<div><h3>Objectives</h3><div>Tooth-grinding procedures generate a significant amount of potentially infectious and pathogenic aerosols, requiring timely and efficient control. The aim of this study was to quantitatively assess the effectiveness of an integrated capture and inactivation approach for controlling aerosol contamination within a dental clinic.</div></div><div><h3>Materials and methods</h3><div>A novel device was developed for the efficient capture of suspended particles and the inactivation of pathogenic microorganisms during tooth-grinding procedures in a controlled clinical dental setting. Six intact molars were pretreated to form uniformly sized cylinders prior to the <em>in vitro</em> tooth grinding experiments. Three replicates of the experiment were conducted, with 3 online real-time particle counters placed along the direction of the grinding particle jet at varying distances from the source to measure particle number concentration. High-energy ion tubes were used as low-temperature plasma generators in inactivation experiments against <em>Staphylococcus aureus</em> and <em>Escherichia coli.</em> Two experimental groups were established based on single- and double-layer generator configurations (7 generators per layer), with a single-generator setup serving as the control group. The ORPC data were analysed using descriptive and differential statistics, and the inactivation rates were derived from pre- and post-treatment colony counts and presented as bar charts.</div></div><div><h3>Results</h3><div>The Kruskal-Wallis test results indicated that under negative pressure capture conditions, aerosol contamination levels differed significantly among the 3 sampling locations (<em>P</em> &lt; .05), but all levels were reduced to baseline within 1 minute. Low-temperature plasma generated by a dual-layer generator configuration achieved nearly 100% inactivation of pathogenic microorganisms within 7 minutes.</div></div><div><h3>Conclusions</h3><div>The integrated capture and inactivation approach can effectively interrupt the indoor transmission of grinding aerosols while achieving efficient inactivation of pathogenic microorganisms.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"76 2","pages":"Article 109331"},"PeriodicalIF":3.7,"publicationDate":"2026-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146018439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to ‘Psychosocial Impact of Dental Aesthetics Among Aboriginal and Torres Strait Islander peoples: Mixed-Methods Study Using Psychosocial Impact of Dental Aesthetics Questionnaire’","authors":"Sonia Nath,&nbsp;Johanna Groundwater,&nbsp;Ria Aiyar,&nbsp;Joanne Hedges,&nbsp;Gina Guzzo,&nbsp;Kostas Kapellas,&nbsp;Alexander Pham,&nbsp;Lisa Jamieson","doi":"10.1016/j.identj.2025.109384","DOIUrl":"10.1016/j.identj.2025.109384","url":null,"abstract":"","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"76 2","pages":"Article 109384"},"PeriodicalIF":3.7,"publicationDate":"2026-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145974235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Fiber Type on Fatigue Performance of Fiber-Reinforced Inlay-Retained Dental Prostheses","authors":"Veronika T. Szabó ,&nbsp;Csongor Mészáros ,&nbsp;Balázs Szabó ,&nbsp;Balázs Szabó P ,&nbsp;Gábor Braunitzer ,&nbsp;Dániel Palkovics ,&nbsp;Zsolt Geretovszky ,&nbsp;Eija Säilynoja ,&nbsp;Lippo Lassila ,&nbsp;Sufyan Garoushi ,&nbsp;Márk Fráter","doi":"10.1016/j.identj.2025.109360","DOIUrl":"10.1016/j.identj.2025.109360","url":null,"abstract":"<div><h3>Objectives</h3><div>To assess the effect of using only long- and short-glass fibers or their combination on the fatigue resistance of composite inlay-retained dental prostheses in dissected mandibular molars with different levels of periodontal support.</div></div><div><h3>Materials and Methods</h3><div>Seventy-two mandibular first molars and second premolars were included in our study. Distal halves of extracted molars were kept, and received endodontic treatment. Standardized occluso-distal and mesio-occlusal cavities were prepared, and premolar-molar units were assembled. The edentulous spans were restored by direct inlay-retained composite prostheses fabricated in 3 different ways (n = 12, 6 groups). In groups 1A and 1B, long fibers and a packable composite, in groups 3A and 3B, short fibers, while in groups 2A and 2B both short and long fibers were used. All units were embedded simulating either physiological periodontal support (1A-3A) or furcation involvement (1B-3B). Specimens were subjected to accelerated fatigue-testing through 40,000 cycles or until fracture. Kaplan–Meyer survival analysis and factorial ANOVA were conducted. Fracture mode was evaluated visually and by scanning electron microscopy.</div></div><div><h3>Results</h3><div>Group 2A (mean 39106.00 N, SD ± 2451, survival frequency 83.3%) and 2B (mean 38454.33 N, SD ± 3110, survival frequency 75%) had the highest load-bearing capacity. Factorial ANOVA revealed that the restorative material significantly affected load-bearing capacity (<em>P</em> &lt; .05) regardless of the periodontal support.</div></div><div><h3>Conclusion</h3><div>The combination of long and short fiber-reinforced systems significantly enhanced the fatigue resistance of direct inlay-retained composite prosthesis.</div></div><div><h3>Clinical relevance</h3><div>This approach may counterbalance the weakening effects of impaired periodontal support, thus improving treatment outcomes for patients with compromised molar support.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"76 2","pages":"Article 109360"},"PeriodicalIF":3.7,"publicationDate":"2026-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145974239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}