International Journal of Antimicrobial Agents最新文献

{"title":"International Society of Antimicrobial Chemotherapy (ISAC) News and Information Page","authors":"","doi":"10.1016/j.ijantimicag.2024.107360","DOIUrl":"10.1016/j.ijantimicag.2024.107360","url":null,"abstract":"","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"64 5","pages":"Article 107360"},"PeriodicalIF":4.9,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142526905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of lefamulin 20 µg disk versus broth microdilution when tested against common respiratory pathogens","authors":"Ying Cao ,&nbsp;Jichao Zhu ,&nbsp;Bingshao Liang ,&nbsp;Yan Guo ,&nbsp;Li Ding ,&nbsp;Fupin Hu","doi":"10.1016/j.ijantimicag.2024.107366","DOIUrl":"10.1016/j.ijantimicag.2024.107366","url":null,"abstract":"<div><h3>Objective</h3><div>To evaluate the performance of the disk diffusion test with lefamulin 20 µg compared with the Clinical and Laboratory Standards Institute (CLSI) reference broth microdilution (BMD) method.</div></div><div><h3>Methods</h3><div>A total of 572 clinical stains, including 240 <em>Staphylococcus aureus</em>, 211 <em>Streptococcus pneumoniae</em>, and 121 <em>Haemophilus influenzae</em>, isolated from 71 medical centres from the China Antimicrobial Surveillance Network in 2020. BMD method and disk diffusion methods were performed according to CLSI. Categorical agreement (CA), major error (ME), and very ME (VME) were calculated.</div></div><div><h3>Results</h3><div>Lefamulin showed potent activity against <em>S. aureus, S. pneumoniae,</em> and <em>H. influenzae</em>. Using the BMD method, lefamulin inhibited 97.1% of <em>S. aureus</em> isolates at 0.25 mg/L; seven isolates were not susceptible. For <em>S. pneumoniae</em> and <em>H. influenzae,</em> the percentage of susceptibility to lefamulin was 100% and no non-susceptible strains were found in this study. Compared with the reference BMD method, the CA of the lefamulin 20 µg disk testing was 99.8% (571/572), with 14.3% (1/7) VME and no ME. In our study, VME was determined in <em>S. aureus</em>. For <em>S. pneumoniae</em> and <em>H. influenzae</em>, the VME was not determined due to the lack of lefamulin non-susceptible strains.</div></div><div><h3>Conclusions</h3><div>The lefamulin 20 µg disk diffusion testing showed excellent CA and ME with the reference BMD method for <em>S. aureus, S. pneumoniae,</em> and <em>H. influenzae</em>. The VME exceeding CLSI recommendations may be a bias due to fewer lefamulin non-susceptible isolates. Our results suggest that lefamulin non-susceptible isolates detected by disk diffusion should be confirmed by the reference BMD.</div></div>","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"64 6","pages":"Article 107366"},"PeriodicalIF":4.9,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142499867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Geographic differences in susceptibility profiles of potential non-class B carbapenemase-producing Enterobacterales isolates against ceftazidime-avibactam, meropenem-vaborbactam, colistin, amikacin, gentamicin, and tigecycline: Data from the Antimicrobial Testing Leadership and Surveillance, 2018–2022","authors":"Shio-Shin Jean ,&nbsp;Wen-Chien Ko ,&nbsp;I-Min Liu ,&nbsp;Po-Chuen Hsieh ,&nbsp;Po-Ren Hsueh","doi":"10.1016/j.ijantimicag.2024.107363","DOIUrl":"10.1016/j.ijantimicag.2024.107363","url":null,"abstract":"<div><div>To evaluate the susceptibility profiles of regional meropenem-resistant potential non-class B carbapenemase-producing Enterobacterales (CPE) isolates (without confirmation by phenotypic tests) against important antibiotics, we extracted data from the 2018-2022 Antimicrobial Testing Leadership and Surveillance. This data included susceptibility information of meropenem-resistant potential non-class B CPE isolates against indicated antibiotics – amikacin, gentamicin, ceftazidime-avibactam, colistin, meropenem-vaborbactam, and tigecycline – from sepsis patients hospitalized in intensive care units across six major regions. Carbapenemase-encoding genes of the tested CPE isolates, determined by multiplex PCR and Sanger sequencing, were also analyzed. Susceptibility breakpoints recommended by Clinical and Laboratory Standards Institute 2024 and US FDA criteria (for tigecycline only) against Enterobacterales were employed. A total of 1500 potential non-class B CPE isolates (89% of which were <em>Klebsiella pneumoniae</em>) were tested globally. Resistance rates to amikacin and gentamicin against the evaluated isolates were statistically higher in Africa/the Middle East, Europe, and India compared to other regions. A similar pattern was observed in the susceptibility of these potential CPE isolates to ceftazidime-avibactam and meropenem-vaborbactam. High colistin resistance rates were noted in Asia, Latin America, and Europe (29%–35%). Furthermore, the proportions of potential CPE isolates carrying genes encoding <em>bla</em>_OXA variants were notably higher among the tested CPE isolates in India, Europe, and Africa/the Middle East regions (99.2%, 53.3%, and 96.7%, respectively) compared to other regions. Trends in resistance to important antibiotics among potential non-class B CPE isolates warrant close monitoring.</div></div>","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"64 6","pages":"Article 107363"},"PeriodicalIF":4.9,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142499868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A contribution on the fosfomycin mechanism of resistance in multidrug-resistant organisms","authors":"Stefano Stracquadanio,&nbsp;Stefania Stefani","doi":"10.1016/j.ijantimicag.2024.107364","DOIUrl":"10.1016/j.ijantimicag.2024.107364","url":null,"abstract":"","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"64 6","pages":"Article 107364"},"PeriodicalIF":4.9,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142499866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LuxS/AI-2 system facilitates the dissemination of antibiotic-resistant plasmids in Klebsiella pneumoniae","authors":"Ying Zhou ,&nbsp;Yang Yang ,&nbsp;Chunyang Wu ,&nbsp;Peiyao Zhou ,&nbsp;Haojin Gao ,&nbsp;Bingjie Wang ,&nbsp;Huilin Zhao ,&nbsp;Yanlei Xu ,&nbsp;Fangyou Yu","doi":"10.1016/j.ijantimicag.2024.107361","DOIUrl":"10.1016/j.ijantimicag.2024.107361","url":null,"abstract":"<div><div>Plasmid conjugation is a central mechanism driving the dissemination of antibiotic resistance in <em>Klebsiella pneumoniae</em>. However, the conjugative operon requires specific stimuli for activation. Identifying signals and elucidating the underlying mechanisms is crucial in combating plasmid spread. This study uncovers a key mechanism promoting the dissemination of high-risk plasmids, including IncFII, IncX3, and IncX4 types, in <em>K. pneumoniae</em>. In this study, increased donor density significantly enhanced conjugation, and transcript levels of both conjugation and AI-2 quorum sensing genes were markedly upregulated. Mutating the <em>luxS</em> and <em>lsrR</em> genes in <em>K. pneumoniae</em> 1678 decreased plasmid conjugation efficiency in the <em>1678ΔluxS</em> mutant, and significantly increased plasmid conjugation efficiency in the <em>1678ΔlsrR</em> mutant. RT-qPCR and β-galactosidase assays showed that LsrR represses transcription of relaxosome and T4CP genes, whereas AI-2 (synthesised by LuxS) activates their expression. AlphaFold 3 docking models indicate that LsrR may bind directly to IncX plasmid relaxase promoters, inhibiting their expression. Adding external AI-2 signals revealed no effect on plasmid conjugation when LsrR was absent, confirming the dependence of AI-2 signals on LsrR repression. In conclusion, AI-2-mediated signalling enhances donor density effects on plasmid conjugation by de-repressing LsrR-mediated suppression.</div></div>","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"64 6","pages":"Article 107361"},"PeriodicalIF":4.9,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142499782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Misassignment of patients in meta-analysis of polymyxin B","authors":"Alexandre P. Zavascki","doi":"10.1016/j.ijantimicag.2024.107362","DOIUrl":"10.1016/j.ijantimicag.2024.107362","url":null,"abstract":"","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"64 6","pages":"Article 107362"},"PeriodicalIF":4.9,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142499783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of rifampicin combination therapy for orthopaedic implant-related infections: A systematic review and meta-analysis","authors":"Naomi Kobayashi ,&nbsp;Kazuhiko Matsushita ,&nbsp;Emi Kamono ,&nbsp;Hiroshi Matsumoto ,&nbsp;Natsumi Saka ,&nbsp;Katsufumi Uchiyama ,&nbsp;Kai Suzuki ,&nbsp;Yui Akiyama ,&nbsp;Hiroyuki Onuma ,&nbsp;Koji Yamada","doi":"10.1016/j.ijantimicag.2024.107359","DOIUrl":"10.1016/j.ijantimicag.2024.107359","url":null,"abstract":"<div><h3>Background</h3><div>In vitro, animal, and clinical comparative studies have revealed that combinations of rifampicin with antibacterial agents are effective in the treatment of biofilm-associated infections. This study aimed to assess the effectiveness of rifampicin combination therapies compared with monotherapies without rifampicin in patients with orthopaedic implant-related infections.</div></div><div><h3>Materials and Methods</h3><div>The clinical literature was comprehensively searched for studies assessing the effectiveness of rifampicin combination therapy in patients with orthopaedic implant-related infection. Identified studies were screened based on the Preferred Reporting Items for Systematic Reviews and Meta-analyses flow diagram. The primary outcome was the cure rate and the secondary outcome was the incidence of adverse events. Subgroup analyses were performed based on causative organisms, antibacterial agents, and types of surgical intervention.</div></div><div><h3>Results</h3><div>Twenty-seven studies were identified, including two randomized controlled trials (RCTs) and 25 cohort studies. Rifampicin combinations were associated with significantly higher cure rates than monotherapies in cohort studies, but not in RCTs. Subgroup analyses showed that rifampicin combinations were effective in patients infected with <em>Cutibacterium</em> and <em>Staphylococcus</em> spp.; in patients who underwent debridement, antibiotics, implant retention procedures; and in patients with one-stage revision. Moreover, combinations of rifampicin and fluoroquinolones were more effective than fluoroquinolones alone, while combinating rifampicin with other antibacterial agents showed no significant benefit. Combination treatment did not significantly affect adverse events in either RCTs or observational studies. Risk of bias assessment and summary of findings showed that the certainty of evidence for both RCTs and observational studies was very low.</div></div><div><h3>Conclusions</h3><div>This systematic review and meta-analysis confirmed the potential effectiveness of rifampicin combination therapy for the cure of orthopaedic implant-related infection in some circumstances, although the certainty and quality of evidence were very low.</div></div>","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"64 6","pages":"Article 107359"},"PeriodicalIF":4.9,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142464588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence of extensively-drug-resistant hypervirulent Acinetobacter baumannii isolated from patients with bacteraemia: bacterial phenotype and virulence analysis","authors":"Pek Kee Chen ,&nbsp;Chia-Ying Liu ,&nbsp;Han-Yueh Kuo ,&nbsp;Yi-Tzu Lee ,&nbsp;Yu-Han Liu ,&nbsp;Yen-Zhen Zhang ,&nbsp;Cheng-Yen Kao","doi":"10.1016/j.ijantimicag.2024.107358","DOIUrl":"10.1016/j.ijantimicag.2024.107358","url":null,"abstract":"<div><h3>Objectives</h3><div>Individuals infected with extensively-drug-resistant (XDR) <em>Acinetobacter baumannii</em> are difficult to cure and have a high mortality rate. This study compared the genomic and phenotypic differences between XDR and non-multi-drug-resistant (MDR) <em>A. baumannii</em>, and further characterized hypervirulent XDR <em>A. baumannii</em>.</div></div><div><h3>Methods</h3><div>In total, 1403 acinetobacter isolates were collected from patients with bacteraemia between 1997 and 2015. Antimicrobial susceptibility tests were performed to categorize isolates into non-MDR, MDR and XDR groups. The presence of selected virulence-associated genes was determined by polymerase chain reaction. Bacterial phenotypes, including iron acquisition, biofilm formation, capsule production, and virulence to larvae and mice, were determined.</div></div><div><h3>Results</h3><div>Multi-locus sequence typing revealed a high prevalence of sequence type (ST) 2 (81.6%) and ST129 (18.4%) among 49 XDR isolates, and the STs of 18 non-MDR isolates were more diverse. Virulence-associated phenotypic assays showed that XDR isolates had higher iron acquisition ability, greater capsule production, and virulence to <em>Galleria mellonella</em> larvae. However, their ability to form biofilm was lower compared with that of non-MDR isolates. XDR isolates were more likely to have virulence genes (<em>tonB, hemO, abaI</em> and <em>ptk</em>), while non-MDR isolates were more likely to have <em>pld</em> and <em>ompA</em> genes. Twenty-one XDR isolates that had a &lt;20% larvae survival rate after 7 days post-infection were defined as hypervirulent XDR isolates. Among them, isolates 1677 (ST129) and 929-1 (ST2) caused the death of all infected mice within 2 days.</div></div><div><h3>Conclusion</h3><div>Some subpopulations of highly-drug-resistant ST2 isolates exhibit high virulence. As such, it is of utmost importance to continue monitoring the spread of hypervirulent XDR <em>A. baumannii</em> isolates.</div></div>","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"64 6","pages":"Article 107358"},"PeriodicalIF":4.9,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142464589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thrice is nice: Thrice weekly versus daily ertapenem in patients on haemodialysis","authors":"Surafel G. Mulugeta ,&nbsp;Andrew Mannino ,&nbsp;Nada Aref ,&nbsp;Vince Procopio ,&nbsp;Suzette Gendjar ,&nbsp;Shaina Vincent","doi":"10.1016/j.ijantimicag.2024.107355","DOIUrl":"10.1016/j.ijantimicag.2024.107355","url":null,"abstract":"","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"64 6","pages":"Article 107355"},"PeriodicalIF":4.9,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142400208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacodynamic Target Attainment at Infection Site During Treatment of Post-Neurosurgical Ventriculitis Caused by Carbapenem-Resistant Klebsiella pneumoniae with Ceftazidime–Avibactam–Based Regimens: a case report","authors":"Yinru Chen ,&nbsp;Wanzhen Li ,&nbsp;Xiaofen Liu ,&nbsp;Yan Chen ,&nbsp;Jing Zhang ,&nbsp;Nanyang Li ,&nbsp;Lei Yang","doi":"10.1016/j.ijantimicag.2024.107356","DOIUrl":"10.1016/j.ijantimicag.2024.107356","url":null,"abstract":"<div><div>A patient developed a post-neurosurgical ventriculitis with carbapenem-resistant <em>Klebsiella pneumoniae</em> and mold, initially treated with ceftazidime/avibactam and voriconazole. A <em>Klebsiella pneumoniae</em> carbapenemase mutation led to therapy adjustment to ceftazidime/avibactam and polymyxin B, achieving cure. Pharmacokinetic/pharmacodynamic analysis highlights effective ceftazidime/avibactam brain penetration and bacterial clearance efficacy.</div></div>","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"64 6","pages":"Article 107356"},"PeriodicalIF":4.9,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142400207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}