Endothelial cell-specific molecule-1 (ESM-1), also known as endocan, is a soluble proteoglycan expressed by the vascular endothelium, which also circulates in the bloodstream. Inflammatory cytokines and proangiogenic growth factors increase its expression, and increased serum levels have been reported in several cancer types and immunocompetent patients with sepsis. The aim of this study was to analyze the expression profile of CXC-chemokines and the effects of ESM-1 gene knockdown in proliferation, migration and CXC-chemokine expression in highly metastatic human prostate PC-3 cells. Expression profiles of CXC-chemokines were analyzed in metastatic PC-3 and non-tumorigenic PWR-1E cells. siRNA-mediated knockdown of ESM-1 was performed into PC-3 cells, which were subsequently tested for cell migration and proliferation. Effect of siRNA transfection on CXC-chemokine expression was further quantified at the transcript and protein level. RT-qPCR analysis and sandwich ELISA assay revealed higher levels of ESM-1 and several CXC-chemokines in metastatic PC-3 cells compared to non-tumorigenic PWR-1E. Transfection of PC-3 cells with ESM-1-siRNA decreased cell migration with no effect on proliferation, and it was accompanied by decrease in the transcript and protein levels of the angiogenic chemokine CXCL3. We report here for the first time the ESM-1 targeting in PC-3 cells, which resulted in decreased migration, which may be related, at least in part, to decreased expression of the angiogenic CXCL3 chemokine, whose expression was found to be reduced in ESM-1-siRNA transfected cells. Additional studies are required to ascertain the biological role of ESM-1 in prostate cancer cells and the link with the expression of CXCL3.
{"title":"ESM-1 siRNA Knockdown Decreased Migration and Expression of CXCL3 in Prostate Cancer Cells.","authors":"Juan Rebollo, Jan Geliebter, Niradiz Reyes","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Endothelial cell-specific molecule-1 (ESM-1), also known as endocan, is a soluble proteoglycan expressed by the vascular endothelium, which also circulates in the bloodstream. Inflammatory cytokines and proangiogenic growth factors increase its expression, and increased serum levels have been reported in several cancer types and immunocompetent patients with sepsis. The aim of this study was to analyze the expression profile of CXC-chemokines and the effects of ESM-1 gene knockdown in proliferation, migration and CXC-chemokine expression in highly metastatic human prostate PC-3 cells. Expression profiles of CXC-chemokines were analyzed in metastatic PC-3 and non-tumorigenic PWR-1E cells. siRNA-mediated knockdown of ESM-1 was performed into PC-3 cells, which were subsequently tested for cell migration and proliferation. Effect of siRNA transfection on CXC-chemokine expression was further quantified at the transcript and protein level. RT-qPCR analysis and sandwich ELISA assay revealed higher levels of ESM-1 and several CXC-chemokines in metastatic PC-3 cells compared to non-tumorigenic PWR-1E. Transfection of PC-3 cells with ESM-1-siRNA decreased cell migration with no effect on proliferation, and it was accompanied by decrease in the transcript and protein levels of the angiogenic chemokine CXCL3. We report here for the first time the ESM-1 targeting in PC-3 cells, which resulted in decreased migration, which may be related, at least in part, to decreased expression of the angiogenic CXCL3 chemokine, whose expression was found to be reduced in ESM-1-siRNA transfected cells. Additional studies are required to ascertain the biological role of ESM-1 in prostate cancer cells and the link with the expression of CXCL3.</p>","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":"13 1","pages":"35-42"},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5422643/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35019031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-03-01DOI: 10.59566/ijbs.2017.13043
C. Shruthi, R. Poojya, Swati Ram, Anupama
Patient: This report describes the case of a 68 year old female patient who presented with the chief complaint of difficulty in chewing and poor aesthetics due to missing teeth. The patient was interested in saving the remaining natural teeth and desired minimal tissue coverage from the prosthesis. After consideration of all the factors involved, it was deemed advisable to resort to a palate free maxillary telescopic complete denture and a mandibular removable partial denture. Discussion: Considering the age of the patient and the cost involved, implant supported prosthesis was ruled out as a treatment option for the patient. A telescopic denture was chosen as a favourable treatment option since it overcomes many of the problems posed by conventional complete dentures like progressive bone loss, lower stability and retention, loss of periodontal proprioception and low masticatory efficiency. It also provides minimal tissue coverage and better distribution of forces. Evaluation of occlusion, esthetics, phonetics and comfort after 24 hours, 1 week and 1 month of treatment showed that the patient was happy with the prosthesis and was able to speak and chew well. Conclusion: Telescopic overdentures have better retention and stability as compared to conventional complete dentures. They improve the chewing efficiency, patient comfort and also decrease the alveolar bone resorption. As such they are an excellent alternative to conventional complete denture treatment.
{"title":"Telescopic Overdenture: A Case Report","authors":"C. Shruthi, R. Poojya, Swati Ram, Anupama","doi":"10.59566/ijbs.2017.13043","DOIUrl":"https://doi.org/10.59566/ijbs.2017.13043","url":null,"abstract":"Patient: This report describes the case of a 68 year old female patient who presented with the chief complaint of difficulty in chewing and poor aesthetics due to missing teeth. The patient was interested in saving the remaining natural teeth and desired minimal tissue coverage from the prosthesis. After consideration of all the factors involved, it was deemed advisable to resort to a palate free maxillary telescopic complete denture and a mandibular removable partial denture. Discussion: Considering the age of the patient and the cost involved, implant supported prosthesis was ruled out as a treatment option for the patient. A telescopic denture was chosen as a favourable treatment option since it overcomes many of the problems posed by conventional complete dentures like progressive bone loss, lower stability and retention, loss of periodontal proprioception and low masticatory efficiency. It also provides minimal tissue coverage and better distribution of forces. Evaluation of occlusion, esthetics, phonetics and comfort after 24 hours, 1 week and 1 month of treatment showed that the patient was happy with the prosthesis and was able to speak and chew well. Conclusion: Telescopic overdentures have better retention and stability as compared to conventional complete dentures. They improve the chewing efficiency, patient comfort and also decrease the alveolar bone resorption. As such they are an excellent alternative to conventional complete denture treatment.","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":"116 1","pages":"43 - 47"},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86267813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E-cadherin is a cell surface adhesion molecules that play an important role in tissue differentiation. FGFR1 is expressed in the developing and mature skeleton in patterns suggestive of both unique and redundant function. Expression levels of E-cadherin and FGFR1 during osteoblastogenesis unclear. In this study primary calvarial mouse osteoblasts were differentiated to mature osteoblasts in osteogenic medium. Alkaline phosphatase (ALP) activity, alizarin red staining, gene expression (Runt-related transcription factor 2 (Runx2), collagen 1 (COL1A2), osteocalcin, E-cadherin and FGFR1) and protein expression (E-cadherin and FGFR1) of osteogenic-cultured primary mouse osteoblast were analysed in this study. The osteogenesis capacity of primary osteoblasts was significantly promoted as ALP activity, alizarin red staining, and the relative expression of Runx2 mRNA and COL1A2 mRNA significantly increased during osteoblastogenesis. The results demonstrated that E-cadherin mRNA and protein were expressed in immature osteoblasts (day 7), but not in mature osteoblasts (day 28). In contrast, the expression of FGFR1 mRNA and protein significantly highly expressed in mature osteoblasts (day 28) compared with immature osteoblasts (day 7). In conclusion, this study demonstrated that E-cadherin could be used as a marker for immature osteoblasts, whereas FGFR1 could be used as a marker for mature osteoblasts during in vitro osteoblastogenesis.
e -钙粘蛋白是一种细胞表面粘附分子，在组织分化中起重要作用。FGFR1在发育和成熟的骨骼中以独特和冗余的功能表达。E-cadherin和FGFR1在成骨细胞形成过程中的表达水平尚不清楚。在成骨培养基中，将原代颅骨小鼠成骨细胞分化为成熟成骨细胞。本研究分析了成骨培养原代小鼠成骨细胞碱性磷酸酶(ALP)活性、茜素红染色、基因表达(runt相关转录因子2 (Runx2)、胶原蛋白1 (COL1A2)、骨钙素、E-cadherin和FGFR1)以及蛋白表达(E-cadherin和FGFR1)。成骨过程中，ALP活性、茜素红染色、Runx2 mRNA和COL1A2 mRNA的相对表达量均显著升高，可显著提高原代成骨细胞的成骨能力。结果表明，E-cadherin mRNA和蛋白在未成熟成骨细胞(第7天)中表达，而在成熟成骨细胞(第28天)中不表达。相比之下，FGFR1 mRNA和蛋白在成熟成骨细胞(第28天)中的表达明显高于未成熟成骨细胞(第7天)。总之，本研究表明，E-cadherin可以作为未成熟成骨细胞的标记物，而FGFR1可以作为体外成骨过程中成熟成骨细胞的标记物。
{"title":"E-Cadherin and FGFR1 Expression in Mouse Osteoblastogenesis in Normoxic Cultures.","authors":"Osama Al-Amer","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>E-cadherin is a cell surface adhesion molecules that play an important role in tissue differentiation. FGFR1 is expressed in the developing and mature skeleton in patterns suggestive of both unique and redundant function. Expression levels of E-cadherin and FGFR1 during osteoblastogenesis unclear. In this study primary calvarial mouse osteoblasts were differentiated to mature osteoblasts in osteogenic medium. Alkaline phosphatase (ALP) activity, alizarin red staining, gene expression (Runt-related transcription factor 2 (Runx2), collagen 1 (COL1A2), osteocalcin, E-cadherin and FGFR1) and protein expression (E-cadherin and FGFR1) of osteogenic-cultured primary mouse osteoblast were analysed in this study. The osteogenesis capacity of primary osteoblasts was significantly promoted as ALP activity, alizarin red staining, and the relative expression of Runx2 mRNA and COL1A2 mRNA significantly increased during osteoblastogenesis. The results demonstrated that E-cadherin mRNA and protein were expressed in immature osteoblasts (day 7), but not in mature osteoblasts (day 28). In contrast, the expression of FGFR1 mRNA and protein significantly highly expressed in mature osteoblasts (day 28) compared with immature osteoblasts (day 7). In conclusion, this study demonstrated that E-cadherin could be used as a marker for immature osteoblasts, whereas FGFR1 could be used as a marker for mature osteoblasts during <i>in vitro</i> osteoblastogenesis.</p>","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":"13 1","pages":"13-19"},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5422640/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35018518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dioscorea hispida (D.hispida) is the most well-known starchy tuber in Malaysia and called 'ubi gadong'. Despite concerns over toxicity effects, the tuber is known to possess therapeutic values due to the presence of bioactive compounds such as saponins. This study was performed to identify the changes in gene expression profiles associated with hepatoxicity in pregnant rat treated with D.hispida using RT² Profiler PCR Array. The identification of steroidal saponins from D.hispida was carried out by UHPLC/MS method. Treatment of D.hispida caused mortality when dosage above 2000 mg/kg b.w. was given to pregnant rats. The PCR array showed that several genes were significantly up and down-regulated upon treatment with D.hispida. Treatment of D.hispida at 2000 mg/kg b.w leads to significant upregulation of several genes such as Btg2, Gsr, L2hgdn, S100a8, Slc17a3, Bhmt, Cd68, Cyp1a2 whereas several genes were downregulated such as Abcb1a, Aldoa, Cdc14b, Icam1, Krt18, Hpn and Maob. The consumption of D.hispida extract when taken at lower dosage of 2000 mg/kg may not be harmful to rats. D.hispida extract given at the highest dosage to pregnant rats caused alterations of several genes categorized in different hepatotoxic group functions such as necrosis, cholestasis and phospholipodisis.
{"title":"Gene Expression Profiling associated with Hepatoxicity in Pregnant Rats treated with Ubi Gadong (<i>Dioscorea hispida</i>) Extract.","authors":"Ezarul Faradianna Lokman, Hussin Muhammad, Norizah Awang, Maizatul Hasyima Omar, Fazliana Mansor, Fatin Saparuddin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p><i>Dioscorea hispida</i> (<i>D.hispida</i>) is the most well-known starchy tuber in Malaysia and called 'ubi gadong'. Despite concerns over toxicity effects, the tuber is known to possess therapeutic values due to the presence of bioactive compounds such as saponins. This study was performed to identify the changes in gene expression profiles associated with hepatoxicity in pregnant rat treated with <i>D.hispida</i> using RT² Profiler PCR Array. The identification of steroidal saponins from <i>D.hispida</i> was carried out by UHPLC/MS method. Treatment of <i>D.hispida</i> caused mortality when dosage above 2000 mg/kg b.w. was given to pregnant rats. The PCR array showed that several genes were significantly up and down-regulated upon treatment with <i>D.hispida</i>. Treatment of <i>D.hispida</i> at 2000 mg/kg b.w leads to significant upregulation of several genes such as Btg2, Gsr, L2hgdn, S100a8, Slc17a3, Bhmt, Cd68, Cyp1a2 whereas several genes were downregulated such as Abcb1a, Aldoa, Cdc14b, Icam1, Krt18, Hpn and Maob. The consumption of <i>D.hispida</i> extract when taken at lower dosage of 2000 mg/kg may not be harmful to rats. <i>D.hispida</i> extract given at the highest dosage to pregnant rats caused alterations of several genes categorized in different hepatotoxic group functions such as necrosis, cholestasis and phospholipodisis.</p>","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":"13 1","pages":"26-34"},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5422642/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35018520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-03-01DOI: 10.59566/ijbs.2017.13035
J. Rebollo, J. Geliebter, N. Reyes
Endothelial cell-specific molecule-1 (ESM-1), also known as endocan, is a soluble proteoglycan expressed by the vascular endothelium, which also circulates in the bloodstream. Inflammatory cytokines and proangiogenic growth factors increase its expression, and increased serum levels have been reported in several cancer types and immunocompetent patients with sepsis. The aim of this study was to analyze the expression profile of CXC-chemokines and the effects of ESM-1 gene knockdown in proliferation, migration and CXC-chemokine expression in highly metastatic human prostate PC-3 cells. Expression profiles of CXC-chemokines were analyzed in metastatic PC-3 and non-tumorigenic PWR-1E cells. siRNA-mediated knockdown of ESM-1 was performed into PC-3 cells, which were subsequently tested for cell migration and proliferation. Effect of siRNA transfection on CXC-chemokine expression was further quantified at the transcript and protein level. RT-qPCR analysis and sandwich ELISA assay revealed higher levels of ESM-1 and several CXC-chemokines in metastatic PC-3 cells compared to non-tumorigenic PWR-1E. Transfection of PC-3 cells with ESM-1-siRNA decreased cell migration with no effect on proliferation, and it was accompanied by decrease in the transcript and protein levels of the angiogenic chemokine CXCL3. We report here for the first time the ESM-1 targeting in PC-3 cells, which resulted in decreased migration, which may be related, at least in part, to decreased expression of the angiogenic CXCL3 chemokine, whose expression was found to be reduced in ESM-1-siRNA transfected cells. Additional studies are required to ascertain the biological role of ESM-1 in prostate cancer cells and the link with the expression of CXCL3.
{"title":"ESM-1 siRNA Knockdown Decreased Migration and Expression of CXCL3 in Prostate Cancer Cells","authors":"J. Rebollo, J. Geliebter, N. Reyes","doi":"10.59566/ijbs.2017.13035","DOIUrl":"https://doi.org/10.59566/ijbs.2017.13035","url":null,"abstract":"Endothelial cell-specific molecule-1 (ESM-1), also known as endocan, is a soluble proteoglycan expressed by the vascular endothelium, which also circulates in the bloodstream. Inflammatory cytokines and proangiogenic growth factors increase its expression, and increased serum levels have been reported in several cancer types and immunocompetent patients with sepsis. The aim of this study was to analyze the expression profile of CXC-chemokines and the effects of ESM-1 gene knockdown in proliferation, migration and CXC-chemokine expression in highly metastatic human prostate PC-3 cells. Expression profiles of CXC-chemokines were analyzed in metastatic PC-3 and non-tumorigenic PWR-1E cells. siRNA-mediated knockdown of ESM-1 was performed into PC-3 cells, which were subsequently tested for cell migration and proliferation. Effect of siRNA transfection on CXC-chemokine expression was further quantified at the transcript and protein level. RT-qPCR analysis and sandwich ELISA assay revealed higher levels of ESM-1 and several CXC-chemokines in metastatic PC-3 cells compared to non-tumorigenic PWR-1E. Transfection of PC-3 cells with ESM-1-siRNA decreased cell migration with no effect on proliferation, and it was accompanied by decrease in the transcript and protein levels of the angiogenic chemokine CXCL3. We report here for the first time the ESM-1 targeting in PC-3 cells, which resulted in decreased migration, which may be related, at least in part, to decreased expression of the angiogenic CXCL3 chemokine, whose expression was found to be reduced in ESM-1-siRNA transfected cells. Additional studies are required to ascertain the biological role of ESM-1 in prostate cancer cells and the link with the expression of CXCL3.","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":"17 1","pages":"35 - 42"},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78201687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-02-21DOI: 10.59566/ijbs.2017.13074
C. Alvarado-Esquivel, Edith Maldonado-Soto, L. F. Sánchez-Anguiano, J. Hernández-Tinoco, Agar Ramos-Nevárez, Sandra Margarita Cerrillo-Soto, Ada Agustina Sandoval-Carrilo, J. Salas-Pacheco, E. Antuna-Salcido, S. Estrada-Martínez, Carlos Alberto Guido-Arreola
The association between T. gondii infection and obesity has been scantly studied. Through an age-, and gender-matched case-control study, we determined the association of T. gondii infection and obesity using serological and molecular methods. Cases included 203 persons with obesity, and controls included 203 persons without obesity. Participants were tested for the presence of anti-Toxoplasma IgG antibodies using an enzyme-linked immunoassay (EIA). IgG seropositive individuals were further tested for the presence of anti-T. gondii IgM antibodies using an EIA, and T. gondii DNA by polymerase chain reaction (PCR). Anti-T. gondii IgG antibodies were found in 16 (7.9%) of the 203 cases and in 18 (8.9%) of the 203 controls (OR=0.87; 95% CI: 0.43-1.77; P=0.72). One (6.3%) of the 16 anti-T. gondii IgG seropositive cases and 6 (33.3%) of the 18 anti-T. gondii IgG seropositive controls were positive for IgM (P=0.09). Mean body mass index (35.5 ± 4.5) in T. gondii seropositive cases was similar (P=0.57) to that (36.1 ± 4.5) found in T. gondii seronegative cases. Stratification by obesity classes (I, II, and III) did not reveal differences (P>0.05) in seroprevalences (7.8%, 7.9%, and 8.1%, respectively) or high (>150 IU/ml) IgG antibody levels (3.3%, 3.9%, and 2.7%, respectively). PCR was positive in 5 (31.3%) of 16 cases, and in 5 (27.8%) of 18 controls examined (P=1.0). We found no serological or molecular evidence of an association between T. gondii infection and obesity in people attending a public health center in the northern Mexican city of Durango.
{"title":"Lack of Serological and Molecular Association between Toxoplasma Gondii Exposure and Obesity: A Case-Control Study","authors":"C. Alvarado-Esquivel, Edith Maldonado-Soto, L. F. Sánchez-Anguiano, J. Hernández-Tinoco, Agar Ramos-Nevárez, Sandra Margarita Cerrillo-Soto, Ada Agustina Sandoval-Carrilo, J. Salas-Pacheco, E. Antuna-Salcido, S. Estrada-Martínez, Carlos Alberto Guido-Arreola","doi":"10.59566/ijbs.2017.13074","DOIUrl":"https://doi.org/10.59566/ijbs.2017.13074","url":null,"abstract":"The association between T. gondii infection and obesity has been scantly studied. Through an age-, and gender-matched case-control study, we determined the association of T. gondii infection and obesity using serological and molecular methods. Cases included 203 persons with obesity, and controls included 203 persons without obesity. Participants were tested for the presence of anti-Toxoplasma IgG antibodies using an enzyme-linked immunoassay (EIA). IgG seropositive individuals were further tested for the presence of anti-T. gondii IgM antibodies using an EIA, and T. gondii DNA by polymerase chain reaction (PCR). Anti-T. gondii IgG antibodies were found in 16 (7.9%) of the 203 cases and in 18 (8.9%) of the 203 controls (OR=0.87; 95% CI: 0.43-1.77; P=0.72). One (6.3%) of the 16 anti-T. gondii IgG seropositive cases and 6 (33.3%) of the 18 anti-T. gondii IgG seropositive controls were positive for IgM (P=0.09). Mean body mass index (35.5 ± 4.5) in T. gondii seropositive cases was similar (P=0.57) to that (36.1 ± 4.5) found in T. gondii seronegative cases. Stratification by obesity classes (I, II, and III) did not reveal differences (P>0.05) in seroprevalences (7.8%, 7.9%, and 8.1%, respectively) or high (>150 IU/ml) IgG antibody levels (3.3%, 3.9%, and 2.7%, respectively). PCR was positive in 5 (31.3%) of 16 cases, and in 5 (27.8%) of 18 controls examined (P=1.0). We found no serological or molecular evidence of an association between T. gondii infection and obesity in people attending a public health center in the northern Mexican city of Durango.","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":"19 1","pages":"74 - 78"},"PeriodicalIF":0.0,"publicationDate":"2017-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82537531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-12-01DOI: 10.59566/ijbs.2016.12130
H. S. Mohammed, Sumeet Singh, Prasad A. Hari, G. Amarnath, Vinaya Kundapur, Naveed Pasha, M. Anand
Background and objective: Chemical cleansing by denture cleansers is first choice for denture plaque control. The most common problems while using denture cleansers are hardening, porosity, odor sorption, water sorption, solubility, and colour change, bacterial and fungal growth. Chemical cleansing procedures have been found to have an effect on the physical and mechanical properties of denture liners. Thus, this study was conducted to evaluate the effect of commercially available denture cleansers on surface hardness and roughness of acrylic and silicon based denture liners at various time interval. Method: Two autopolymerising denture liners Kooliner (acrylic) and GC reline soft (silicon) were tested with two commercially available denture cleansers, polident and efferdent plus. Total of 120 specimens were prepared and all the specimens were divided into six groups based on the relining materials and denture cleansers used. Surface hardness and surface roughness was tested using Shore A durometer and profilometer respectively at the end of day 1, day 7, day 30 and day 90. All the specimens were stored in artificial saliva throughout the study. Cleanser solution was prepared daily by adding Polident and Efferdent plus denture cleanser tablet into 250ml of enough very warm (not hot) water. Acrylic and silicon liner groups were cleansed in a solution of denture cleanser and water for 15 minutes daily, rinsed with water and stored in artificial saliva at room temperature. The data was analyzed with one way ANOVA and independent t-test. Result: The acrylic soft lining showed gradual hardening and increase in surface roughness after immersion in denture cleanser and also with time. Acrylic liner material showed maximum hardness and roughness with Polident followed by Efferdent plus and water (control group). Silicone lining material showed a slight difference in hardness and roughness between the test group and control group. There was a slight increase in hardness in all the groups with time. Very slight increase in mean surface roughness of all the silicon liner groups from day 1 to day 90 was observed. A statistically significant change was noted between and within the all silicon liner groups on day 7, day 30 and day 90. Conclusion: The average surface hardness and surface roughness were lower in silicon liner material than acrylic liner material. Maximum surface roughness was noted by Polident followed by Efferdent Plus and Water for both acrylic liner group and silicon liner group.
{"title":"Evaluate the Effect of Commercially Available Denture Cleansers on Surface Hardness and Roughness of Denture Liners at Various Time Intervals","authors":"H. S. Mohammed, Sumeet Singh, Prasad A. Hari, G. Amarnath, Vinaya Kundapur, Naveed Pasha, M. Anand","doi":"10.59566/ijbs.2016.12130","DOIUrl":"https://doi.org/10.59566/ijbs.2016.12130","url":null,"abstract":"Background and objective: Chemical cleansing by denture cleansers is first choice for denture plaque control. The most common problems while using denture cleansers are hardening, porosity, odor sorption, water sorption, solubility, and colour change, bacterial and fungal growth. Chemical cleansing procedures have been found to have an effect on the physical and mechanical properties of denture liners. Thus, this study was conducted to evaluate the effect of commercially available denture cleansers on surface hardness and roughness of acrylic and silicon based denture liners at various time interval. Method: Two autopolymerising denture liners Kooliner (acrylic) and GC reline soft (silicon) were tested with two commercially available denture cleansers, polident and efferdent plus. Total of 120 specimens were prepared and all the specimens were divided into six groups based on the relining materials and denture cleansers used. Surface hardness and surface roughness was tested using Shore A durometer and profilometer respectively at the end of day 1, day 7, day 30 and day 90. All the specimens were stored in artificial saliva throughout the study. Cleanser solution was prepared daily by adding Polident and Efferdent plus denture cleanser tablet into 250ml of enough very warm (not hot) water. Acrylic and silicon liner groups were cleansed in a solution of denture cleanser and water for 15 minutes daily, rinsed with water and stored in artificial saliva at room temperature. The data was analyzed with one way ANOVA and independent t-test. Result: The acrylic soft lining showed gradual hardening and increase in surface roughness after immersion in denture cleanser and also with time. Acrylic liner material showed maximum hardness and roughness with Polident followed by Efferdent plus and water (control group). Silicone lining material showed a slight difference in hardness and roughness between the test group and control group. There was a slight increase in hardness in all the groups with time. Very slight increase in mean surface roughness of all the silicon liner groups from day 1 to day 90 was observed. A statistically significant change was noted between and within the all silicon liner groups on day 7, day 30 and day 90. Conclusion: The average surface hardness and surface roughness were lower in silicon liner material than acrylic liner material. Maximum surface roughness was noted by Polident followed by Efferdent Plus and Water for both acrylic liner group and silicon liner group.","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":"186 1","pages":"130 - 142"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74511831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-12-01DOI: 10.59566/ijbs.2016.12125
Mahmood Akbaryan, F. Darabi, Z. Soltani
Dermatomyositis is an idiopathic inflammatory myopathy that cause skin and muscle complications. The ethiology is not understood well yet. Released cytokines including interferon and interleukins are suggested to make inflammatory responses in the skin or muscle. Muscle weakness and skin lesions including heliotrope rash, shawl sign and Gottron’s papules are the most common symptoms. A biopsy (muscle or skin) is always the most reliable method for diagnosis. Corticosteroids in association with immunosuppressive agents are used as standard treatment. The patient was a 30 years old woman who got involved with dermatomyositis for 10 years. She has been under therapy with Methotrexate, Prednisolon and Azathioprine until she came to us suffering from progressive skin lesions. Experiments and examinations were normal except the lesions and detected lipoatrophy. Because of immune cells infiltration and observations necrotizing vasculitis was diagnosed. After three month of high dose prednisolon and intravenous cyclophosphamide therapy the lesions vanished remarkable. True and immediate diagnosis gives physicians the chance not only to assess the best treatment but have adequate time to apply the procedure. However shortening the therapy and diminishing morbidity of the disease need more investigations and efforts.
{"title":"Dermatomyositis Leading to Necrotizing Vasculitis: A Perfect Response to Applied Therapy","authors":"Mahmood Akbaryan, F. Darabi, Z. Soltani","doi":"10.59566/ijbs.2016.12125","DOIUrl":"https://doi.org/10.59566/ijbs.2016.12125","url":null,"abstract":"Dermatomyositis is an idiopathic inflammatory myopathy that cause skin and muscle complications. The ethiology is not understood well yet. Released cytokines including interferon and interleukins are suggested to make inflammatory responses in the skin or muscle. Muscle weakness and skin lesions including heliotrope rash, shawl sign and Gottron’s papules are the most common symptoms. A biopsy (muscle or skin) is always the most reliable method for diagnosis. Corticosteroids in association with immunosuppressive agents are used as standard treatment. The patient was a 30 years old woman who got involved with dermatomyositis for 10 years. She has been under therapy with Methotrexate, Prednisolon and Azathioprine until she came to us suffering from progressive skin lesions. Experiments and examinations were normal except the lesions and detected lipoatrophy. Because of immune cells infiltration and observations necrotizing vasculitis was diagnosed. After three month of high dose prednisolon and intravenous cyclophosphamide therapy the lesions vanished remarkable. True and immediate diagnosis gives physicians the chance not only to assess the best treatment but have adequate time to apply the procedure. However shortening the therapy and diminishing morbidity of the disease need more investigations and efforts.","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":"25 1","pages":"125 - 129"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86780622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-12-01DOI: 10.59566/ijbs.2016.12149
M. Ahmadinejad, K. Ahmadi, I. Ahmadinejad, Amir Masoud Hashemian, Peyman Khademhoseini
Background and Objectives: Pilonidal sinus is a disease in the sacrococcygeal region diagnosed through the purulent discharges of the above-said region. Although the exact pathology and etiology of those suffering from pilonidal is not clear yet, the presence of hair seemingly plays a major role in the process of infection and the granulation tissue. Several techniques have been identified for pilonidal surgery. These techniques primarily fall within two categories: Primary repair and Lay open. One of the setbacks of the primary repair method is the creation of a dead area under the wound which can result in blood accumulation and seroma. To solve the problem of removing the dead space, there are two solutions. The first method utilizes a close suction drain, when the wound discharges are over, the drain is removed. The second technique is called Tie-over where different layers of the wound are pushed close to one another and the dead region vanishes. The present research seeks to compare Tie-over and Closed Suction Drainage methods through random clinical trial in order to introduce the superior technique for faster recovery and reduction of the economic load on the patient. Methods: Some 64 patients suffering from sacral plonidal sinus aging from 15 to 50 in Shohadaye Ashayer Hospital of Khoram Abad in 2013 were selected for the research based on the inclusion criteria. The demographic information of them was collected through questionnaires. The patients were randomly divided into two groups undergoing (A) Tiover and (B) Closed Suction Drainage surgeries. Other information such as return to normal activity and total recovery time was also completed through the questionnaire. SPSS software was used to conduct statistical analysis. Results: The results of the statistical analysis showed that the two groups were similar to one another in terms of age, gender, marital status, job, and literacy. A significant difference was observed only between the level of satisfaction (P-value = 0.035) in groups A and B concerning the factors studied after the operation. The post-operation recurrence of pilonidal sinus among those who smoked cigarettes and had undergone Closed Suction Drainage was significantly greater than the non-smokers (P-value=0.011). As of the group undergoing Tie-over surgery, the difference between the patients’ satisfaction in terms of their age was statistically significant and the highest level of satisfaction was observed among those aging 25 to 34 (90%) (P-value=0.023). Conclusion: In sacrococcygeal pilonidal cyst surgery, no difference was observed except for the difference in the level of satisfaction. More satisfaction was observed using the Tiover method.
{"title":"A Comparison between the Tie-over and Closed Suction Drainage Therapeutic Strategies in Patients Suffering from Sacral Pilonidal Sinus","authors":"M. Ahmadinejad, K. Ahmadi, I. Ahmadinejad, Amir Masoud Hashemian, Peyman Khademhoseini","doi":"10.59566/ijbs.2016.12149","DOIUrl":"https://doi.org/10.59566/ijbs.2016.12149","url":null,"abstract":"Background and Objectives: Pilonidal sinus is a disease in the sacrococcygeal region diagnosed through the purulent discharges of the above-said region. Although the exact pathology and etiology of those suffering from pilonidal is not clear yet, the presence of hair seemingly plays a major role in the process of infection and the granulation tissue. Several techniques have been identified for pilonidal surgery. These techniques primarily fall within two categories: Primary repair and Lay open. One of the setbacks of the primary repair method is the creation of a dead area under the wound which can result in blood accumulation and seroma. To solve the problem of removing the dead space, there are two solutions. The first method utilizes a close suction drain, when the wound discharges are over, the drain is removed. The second technique is called Tie-over where different layers of the wound are pushed close to one another and the dead region vanishes. The present research seeks to compare Tie-over and Closed Suction Drainage methods through random clinical trial in order to introduce the superior technique for faster recovery and reduction of the economic load on the patient. Methods: Some 64 patients suffering from sacral plonidal sinus aging from 15 to 50 in Shohadaye Ashayer Hospital of Khoram Abad in 2013 were selected for the research based on the inclusion criteria. The demographic information of them was collected through questionnaires. The patients were randomly divided into two groups undergoing (A) Tiover and (B) Closed Suction Drainage surgeries. Other information such as return to normal activity and total recovery time was also completed through the questionnaire. SPSS software was used to conduct statistical analysis. Results: The results of the statistical analysis showed that the two groups were similar to one another in terms of age, gender, marital status, job, and literacy. A significant difference was observed only between the level of satisfaction (P-value = 0.035) in groups A and B concerning the factors studied after the operation. The post-operation recurrence of pilonidal sinus among those who smoked cigarettes and had undergone Closed Suction Drainage was significantly greater than the non-smokers (P-value=0.011). As of the group undergoing Tie-over surgery, the difference between the patients’ satisfaction in terms of their age was statistically significant and the highest level of satisfaction was observed among those aging 25 to 34 (90%) (P-value=0.023). Conclusion: In sacrococcygeal pilonidal cyst surgery, no difference was observed except for the difference in the level of satisfaction. More satisfaction was observed using the Tiover method.","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":"52 1","pages":"149 - 154"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77820813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-12-01DOI: 10.59566/ijbs.2016.12143
Adel Johari Moghadam, Saied Azizinejad
Introduction Although cardiac rehabilitation is known as a tool to reduce the overall risk of cardiovascular complications, its specific role in the reduction of hs-CRP as a marker of inflammation and a proven marker of cardiovascular risk needs further investigation. Aim The present study aims at elucidating the effects of a full course of conventional cardiac rehabilitation program for the period of eight weeks, on the levels of hs-CRP in patients who underwent isolated coronary artery bypass surgery. Material and Methods In this case study, 30 consecutive patients who underwent isolated coronary artery bypass surgery (isolated CABGS), and a full 8-week cardiac rehabilitation program in Tehran Heart Center, were investigated. A group of 30 similar patients, who enrolled in the same period of rehabilitation program but did not participate in practice, was considered as a control group. Serum levels of hs-CRP in both groups were measured retrospectively and in similar days before the start of rehabilitation program and at the end of it (or 8 weeks after initial registration for the control group). Results Levels of hs-CRP in the rehabilitation group and control group were 5.9 7.7 and 6.3 6.9 respectively before start of the program which was not statistically meaningful (P-Value = 0.833). However, after the program, level of hs-CRP in the two tested groups changed to 2.3 5.1 and 5.7 6.1 respectively which showed a meaningful correlation (P-Value = 0.023). These results also showed that decrease in hs-CRP level in the rehabilitated group but not in the control group was statistically meaningful (with P-Value of 0.037 and 0.0723 respectively). Conclusion In patients undergoing coronary bypass surgery, participating in a full course of cardiac rehabilitation for 8 weeks has resulted in a significant reduction in hs-CRP levels as a marker of cardiovascular risk.
{"title":"Study of High Sensitive C-Reactive Protein (HS-CRP) After Cardiac Rehabilitation Program in Patients Undergoing Isolated CABG","authors":"Adel Johari Moghadam, Saied Azizinejad","doi":"10.59566/ijbs.2016.12143","DOIUrl":"https://doi.org/10.59566/ijbs.2016.12143","url":null,"abstract":"Introduction Although cardiac rehabilitation is known as a tool to reduce the overall risk of cardiovascular complications, its specific role in the reduction of hs-CRP as a marker of inflammation and a proven marker of cardiovascular risk needs further investigation. Aim The present study aims at elucidating the effects of a full course of conventional cardiac rehabilitation program for the period of eight weeks, on the levels of hs-CRP in patients who underwent isolated coronary artery bypass surgery. Material and Methods In this case study, 30 consecutive patients who underwent isolated coronary artery bypass surgery (isolated CABGS), and a full 8-week cardiac rehabilitation program in Tehran Heart Center, were investigated. A group of 30 similar patients, who enrolled in the same period of rehabilitation program but did not participate in practice, was considered as a control group. Serum levels of hs-CRP in both groups were measured retrospectively and in similar days before the start of rehabilitation program and at the end of it (or 8 weeks after initial registration for the control group). Results Levels of hs-CRP in the rehabilitation group and control group were 5.9 7.7 and 6.3 6.9 respectively before start of the program which was not statistically meaningful (P-Value = 0.833). However, after the program, level of hs-CRP in the two tested groups changed to 2.3 5.1 and 5.7 6.1 respectively which showed a meaningful correlation (P-Value = 0.023). These results also showed that decrease in hs-CRP level in the rehabilitated group but not in the control group was statistically meaningful (with P-Value of 0.037 and 0.0723 respectively). Conclusion In patients undergoing coronary bypass surgery, participating in a full course of cardiac rehabilitation for 8 weeks has resulted in a significant reduction in hs-CRP levels as a marker of cardiovascular risk.","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":"9 1","pages":"143 - 148"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79544108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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