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Evaluation of the Expression of the CK-18 Tumor Marker in Blood Samples of Breast Cancer Patients using Real-Time PCR Real-Time PCR检测乳腺癌患者血液中CK-18肿瘤标志物的表达
Pub Date : 2019-04-01 DOI: 10.33945/SAMI/IJABBR.2019.2.5
A. Gharibi, Roudabeh Behzadi Andouhjerdi
Background: Cytokeratins (CK) are the largest subgroup of interstitial proteins, and their expression changes during the development of cancer. Cytokeratin (CK-18) is one of the major proteins of the epithelial cell skeleton. The expression potential of the CK-18 gene was studied in this research as a molecular biomarker for the diagnosis of breast cancer in the circulatory system using the Real-Time PCR technique. Methods: Blood samples of breast cancer patients and healthy individuals (as the control group) were collected from the Cancer Institute of Imam Khomeini Hospital, Tehran, Iran and their RNA was extracted. In the next step, the cDNA molecule was synthesized using a reverse transcriptase enzyme (RT), and gene-specific primers were designed and synthesized. Then, the expression of CK-18 tumor marker was evaluated by the real-time PCR technique; finally, the data obtained from the cancer samples and the control group was analyzed by the SPSS software. Results: CK-18 expression was observed and measured in the patients, serum .In addition, according to the disease grade, the CK-18 expression was different in the patients, serum. Conclusions: The findings of this research can be used in future studies exploring the mechanism of action of this gene as a suitable target for the treatment of breast cancer.
背景:细胞角蛋白(Cytokeratins, CK)是间质蛋白中最大的亚群,其表达在癌症的发展过程中发生变化。细胞角蛋白(CK-18)是上皮细胞骨架的主要蛋白之一。本研究利用Real-Time PCR技术研究CK-18基因在循环系统中的表达潜力,作为乳腺癌诊断的分子生物标志物。方法:采集伊朗德黑兰伊玛目霍梅尼医院癌症研究所乳腺癌患者和健康人群(对照组)的血液标本,提取RNA。下一步,利用逆转录酶(RT)合成cDNA分子,设计并合成基因特异性引物。采用实时荧光定量PCR技术检测CK-18肿瘤标志物的表达;最后,用SPSS软件对癌症样本和对照组的数据进行分析。结果:观察并测定了CK-18在患者、血清中的表达,并根据病情分级,CK-18在患者、血清中的表达存在差异。结论:本研究结果可为进一步探索该基因作为治疗乳腺癌的合适靶点的作用机制提供参考。
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引用次数: 1
Expression of FGF19 gene as an effective gene for the invasive behavior of cancer cells in patients with colorectal cancer FGF19基因在结直肠癌患者中作为癌细胞侵袭行为有效基因的表达
Pub Date : 2019-04-01 DOI: 10.33945/SAMI/IJABBR.2019.2.6
R. Yavari, S. Bostanabad, A. Gharibi
Introduction Colorectal cancer is the second and third most common cancer in women and men respectively. Early diagnosis of illness will reduce pain and costs for patients. In this study, the expression of fgf19 gene in different individuals with colorectal cancer, and also the relationship between the expression of this gene and the degree and stage of cancer cells, will be evaluated.Methods A total of 60 samples were collected by a surgeon from cancerous tissue and healthy marginal tissue of patients with colorectal cancer. Extraction of RNA was accomplished by a Trizol solution. In the next step, cDNA molecule was synthesized using reverse transcriptase enzyme (RT), and gene-specific primers were designed and synthesized. Then the expression of FGF19 gene was evaluated by Real-time PCR technique. Finally, the data obtained from cancerous tissue and healthy marginal tissue were analyzed by spss software.Result The expression of fgf19 in tumor tissue and healthy marginal tissue was measured quantitatively, which increased by 56.7 percent.Conclusion The results of this study indicate that fgf19 as a molecular biomarker plays a significant role in the progression of cancer, and therefore can be considered as a factor in the screening, early detection, prognosis and predictor of the tumor.
结直肠癌分别是女性和男性中第二和第三常见的癌症。疾病的早期诊断将减少患者的痛苦和费用。本研究将评估fgf19基因在不同结直肠癌个体中的表达情况,以及该基因表达与癌细胞的程度和分期之间的关系。方法由一名外科医生从结直肠癌患者的癌组织和健康边缘组织中抽取60份标本。RNA的提取由Trizol溶液完成。下一步,利用逆转录酶(RT)合成cDNA分子,设计并合成基因特异性引物。采用Real-time PCR技术检测FGF19基因的表达。最后用spss软件对癌组织和健康边缘组织的数据进行分析。结果fgf19在肿瘤组织和健康边缘组织中的表达量均增加了56.7%。结论fgf19作为一种分子生物标志物,在肿瘤的进展过程中发挥着重要作用,可作为肿瘤的筛查、早期发现、预后和预测因素之一。
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引用次数: 2
The Effect of Eating Even or Odd Number of Dates, on Blood Glucose Level 吃偶数或奇数个枣对血糖水平的影响
Pub Date : 2019-04-01 DOI: 10.33945/SAMI/IJABBR.2019.2.9
M. Al-Nuaimi, Yossra K. Al-Robaiaay, T. F. Al-Auqbi
Objectives: There is a common belief among Muslims, to consume dates in odd number rather than even, based on cultural and religious claims; although nobody knows its effect on blood glucose level or other metabolic effects in the body.The aim of our study is to illuminate the changes that affect the 2-hrs postprandial serum glucose level after the consumption of an odd number of dates among healthy young persons and even number. Method: Intervention study was used to compare 2 groups, Fasting glucose levels was measured for all participants; group A (42 participants), who consumed even number of dates all at Tamer-stage, (net weight about 50 grams); and group B, (42 participants), presumed to consume an odd number of dates (net weight about 40 grams). Two-hrs postprandial blood glucose levels were measured altogether. Blood glucose levels were determined and compared as a means. Statistical analyses were performed using SPSS version 22.Result: The fasting serum glucose of group A and B participants were 91.65±13.61 and 93.69±8.79 mg/dl, respectively, with an insignificant statistical difference between groups (P= 0.42327). Subsequently, the 2hrs Postprandial Glucose measurements of group A and B were 99.58±19.56 and 100.33±12.204 respectively; indicated an insignificant statistical difference between groups, (P=0.83553). All the participants were remaining within normal ranges of serum glucose level. Conclusion: There is no difference between ingestion of the odd and even number of dates fleshes from the glycemic point of view on the glucose level in fasting and postprandial states.
目标:在穆斯林中有一种共同的信念,基于文化和宗教主张,以奇数而不是偶数来消费日期;虽然没有人知道它对血糖水平的影响或对体内其他代谢的影响。本研究的目的是阐明健康青年吃奇数和偶数个枣子后餐后2小时血糖水平的变化。方法:采用干预研究方法比较两组患者的空腹血糖水平;A组(42名参与者),他们在tamer阶段吃偶数个枣(净重约50克);而B组(42名参与者)则被认为吃奇数个枣(净重约40克)。同时测量餐后两小时的血糖水平。测定并比较血糖水平作为一种手段。采用SPSS 22进行统计分析。结果:A、B组空腹血糖分别为91.65±13.61、93.69±8.79 mg/dl,组间差异无统计学意义(P= 0.42327)。随后,A组和B组餐后2h血糖值分别为99.58±19.56和100.33±12.204;组间差异无统计学意义(P=0.83553)。所有的参与者都保持在正常的血糖水平范围内。结论:从血糖学的角度来看,奇数和偶数红枣肉片对空腹和餐后血糖水平没有差异。
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引用次数: 0
The Therapeutic Effect of Statins on Survival of Patients with Gastrointestinal Cancer: a Review 他汀类药物对胃肠道肿瘤患者生存的影响综述
Pub Date : 2019-03-15 DOI: 10.33945/SAMI/IJABBR.2019.7.203215
A. Olfati, Nasrollah Moradi-kor
Cancer -can significantly have ruining effects on very high of human beings, social, and economic condition. Gastrointestinal (GI) cancer has been reported as one of the most prevalent cancers and the incidence rates of this disorder is increasing across the world. This review explains practical recommendations for the management and improvement of patients with GI cancer by statins. Statins, a group of lipoproteins drugs, are used in medicine to treatment of lipid disorder, hypercholesterolemia reduction and cardiovascular risk. The exact mechanisms of anticancer activity of statins are still unknown. However, in some preclinical and epidemiological studies have reported them to have anticancer properties and therapeutic consequences for GI cancer based on preclinical evidence of their including antihypoxic processes, anti-invasive, induction of growth arrest and proapoptotic, radiosensitizing, angiogenesis and inhibition metastasis. The main objectives of this review paper are: 1. Explain the importance of sickness understanding and prognostic awareness in patients with cell proliferation, induction cell apoptosis, metastasis, angiogenesis, immunity and inflammatory GI cancers receiving statins groups. 2. Explain the importance of statins therapy in low- and middle- risk patients with GI cancers.
癌症对人类、社会和经济状况都有很大的破坏性影响。据报道,胃肠道(GI)癌症是最常见的癌症之一,其发病率在世界范围内呈上升趋势。这篇综述解释了他汀类药物治疗和改善胃肠道癌患者的实用建议。他汀类药物是一组脂蛋白药物,在医学上用于治疗脂质紊乱、降低高胆固醇血症和心血管风险。他汀类药物抗癌活性的确切机制尚不清楚。然而,在一些临床前和流行病学研究中,根据临床前证据,它们具有抗癌特性和对胃肠道癌症的治疗效果,包括抗缺氧过程、抗侵袭、诱导生长停滞和促凋亡、放射增敏、血管生成和抑制转移。本文的主要目的是:1。解释在接受他汀类药物治疗的细胞增殖、诱导细胞凋亡、转移、血管生成、免疫和炎症性胃肠道癌症患者中疾病理解和预后意识的重要性。2. 解释他汀类药物治疗在中低风险胃肠道肿瘤患者中的重要性。
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引用次数: 4
Investigating the Prevalence of Childhood Asthma Symptoms in Khomein Town, Markazi Province, Iran: A Cross-Sectional Study 调查伊朗马卡齐省霍梅因镇儿童哮喘症状的患病率:一项横断面研究
Pub Date : 2019-03-01 DOI: 10.26655/IJABBR.2019.3.1
B. Abdolkarimi, Javad Javaheri, I. Sedighi, Mahshid Garmsiri, S. Sedighi
Asthma is the most common cause of recurrent wheezing, cough and dyspnea in childhood. Delay in diagnosis and lack of appropriate treatment can cause sustained changes which decrease respiratory capacity of children in future. Therefore, identifying the symptoms relating to asthma is the key for timely diagnosis and treatment of this disease. This study was done to investigate asthma incidence in khomein and compare it to other cities of Iran susceptible to asthma, including Tehran. The present study was a descriptive cross-sectional one based on standard questionnaire of International Study of Asthma and Allergies in Childhood (ISAAC). For conducting this study, 1621 students of first grade in some primary schools and junior high schools of khomein were selected through nonrandom sampling and census and were evaluated during 2015-2016. Both groups were studied and evaluated in terms of being affected by asthma and its severity (current and previous wheezing ,night-time cough, talking limitation due to coughing or wheezing, exercise-induced wheezing, nocturnal awaking due to cough). Results were analyzed through the chi-square test. The asthma diagnosed in Khomein students by the physician during the study was 3.5% while 73.2% of the children under study had at least one asthma symptom. One eighth of the students under study had severe asthma. Comparing with polluted Tehran and northern cities of Iran, Asthma symptoms incidence was higher in khomein where the humidity and industrial pollution is less than in those cities and the asthma cases diagnosed by physicians were much fewer than expected asthma incidence.
哮喘是儿童时期反复发作的喘息、咳嗽和呼吸困难的最常见原因。诊断的延误和缺乏适当的治疗可引起持续的变化,从而降低儿童未来的呼吸能力。因此,识别哮喘相关症状是及时诊断和治疗该病的关键。这项研究是为了调查霍梅因的哮喘发病率,并将其与伊朗其他易患哮喘的城市(包括德黑兰)进行比较。本研究是基于国际儿童哮喘和过敏研究(ISAAC)标准问卷的描述性横断面研究。本研究通过非随机抽样和普查的方法,选取霍梅因部分小学和初中的一年级学生1621名,于2015-2016年进行评估。研究和评估两组受哮喘影响及其严重程度(当前和既往的喘息、夜间咳嗽、因咳嗽或喘息引起的说话限制、运动引起的喘息、因咳嗽引起的夜间清醒)。结果通过卡方检验进行分析。霍梅因学生在研究期间被医生诊断为哮喘的比例为3.5%,而73.2%的被研究儿童至少有一种哮喘症状。接受研究的学生中有八分之一患有严重哮喘。与伊朗污染严重的德黑兰和北部城市相比,霍梅因湿度和工业污染较轻,哮喘症状发生率较高,医生诊断的哮喘病例远低于预期的哮喘发病率。
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引用次数: 0
Compare Modeling the effect of Flow Parameters on the Efficiency of Membrane Clarification of Pomegranate Juice Regression Method with Artificial Intelligence Methods 比较模拟流动参数对石榴汁膜澄清效率的影响回归法与人工智能方法
Pub Date : 2019-03-01 DOI: 10.26655/IJABBR.2019.3.3
M. Poudineh
Objective: Pomegranate juice is a fruit native to Iran because of attractive color, smell and value of mineral water is popular fruits in the world. Pomegranate juice has more nutritional value is due to a combination of anthocyanins that reduce the risk of diseases such as cancer. Processes Feta membrane such as microfiltration 1 and 2 are used to clarify beer industry. Methods: The advantage of this method compared to traditional methods require less labor, higher yields and the process is low. Making a mathematical model or artificial intelligence to predict the juice clarification process in membrane systems is a valuable tool in the field of membrane science and technology. Results: These models play an important role in the simulation and optimization of transparency in membrane systems in order to achieve an economic and efficient design Play. Many of the older models Polar models, the osmotic pressure and boundary layer model have been used to simulate the performance tuning 1 fruit juices. Conclusion: In this study, we tried to take advantage of four regression system, fuzzy inference, neural networks, and fuzzy-neural adaptive method for predicting the flow of water permeate the membrane pomegranate transparency in the system assessed
目的:石榴汁是一种原产于伊朗的水果,因其诱人的色泽、气味和矿泉水的价值而受到世界各国的欢迎。石榴汁之所以有更多的营养价值,是因为它含有花青素,可以降低患癌症等疾病的风险。微滤工艺1和微滤工艺2用于澄清啤酒工业。方法:与传统方法相比,该方法具有人工少、收率高、工艺低的优点。建立数学模型或人工智能来预测膜系统中的果汁澄清过程是膜科学与技术领域的一个有价值的工具。结果:这些模型对膜系统透明度的模拟和优化具有重要作用,可实现经济高效的设计。许多旧的模型,极地模型,渗透压和边界层模型已经被用来模拟果汁的性能调整。结论:在本研究中,我们尝试利用四种回归系统、模糊推理、神经网络和模糊神经自适应方法来预测水通过石榴膜的流量,并对系统的透明度进行评估
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引用次数: 0
Nutritional quality in rice grain and their relations to grain morphological traits 稻米营养品质及其与稻米形态性状的关系
Pub Date : 2019-01-01 DOI: 10.26655/IJABBR.2019.1.1
R. Heydari, N. Bagheri, N. B. Jelodar, H. N. Zarrini
Cereal grains are considered by consumers due to effect on health because of their antioxidant capacity, phenolic content and other phytochemicals. For this purpose, an experiment was conducted to evaluate of morphological and nutritional traits on 10 rice genotypes. Analysis of variance showed a significant different among the genotypes for all traits. Onda, Gharib, Fajr and L2 genotypes had the highest ranks for nutritional quality. Also, the results showed that positive correlation between antioxidant capacity, phenolic content, soluble carbohydrate and Zn content, while there was the negative correlation between grain length and grain length to width ratio with above traits. Thus, the nutritional quality could be indirectly selected based on grain length. PCA analysis resulted five components that determined 89.22% of the total variation, so that scatter plot based on the two first components, the genotypes of Onda, Fajr, Gharib and L2 which have smaller grain length, have better nutritional quality. Therefore, these genotypes can be used in breeding programs.
谷类食品由于其抗氧化能力、酚类物质含量和其他植物化学物质而被消费者认为对健康有影响。为此,对10个水稻基因型的形态和营养性状进行了评价。方差分析表明,各性状基因型间差异显著。Onda、Gharib、Fajr和L2基因型的营养品质排名最高。抗氧化能力、酚类物质含量、可溶性碳水化合物含量和锌含量与上述性状呈显著正相关,而粒长和粒长宽比与上述性状呈显著负相关。因此,可以通过粒长间接选择籽粒的营养品质。主成分分析得到5个分量占总变异的89.22%,因此基于前2个分量的散点图表明,Onda、Fajr、Gharib和L2基因型粒长较小,营养品质较好。因此,这些基因型可用于育种计划。
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引用次数: 0
Analysis of factors affecting the development of an entrepreneurial student 影响创业型学生发展的因素分析
Pub Date : 2019-01-01 DOI: 10.26655/IJABBR.2019.1.5
Feizollah Monavari Fard, Seyed Alireza Dehghan, Amine Lotfian, L. Salehi
The purpose of this paper is to explore the influential factors on entrepreneurial spirit of wood and paper industries students in two universities, namely Agriculture and natural resources faculty of Tehran University and Shahid Rajaiee teacher training University. A sample of 100 students were selected from a target population of 572 students using the Cochran formula. The respondents were asked to provide their answers to a standardized questionnaire. Results indicated that from the students’ perspective such factors as motivation, providence and achievement propensity had the greatest impact on entrepreneurial spirit of the respondents whilst factors like internal control, exception ability and having systematic attitude had the least influence. Moreover concerning the effect of educational and academic factors on entrepreneurial spirit, the most effective items were respectively favorable access to computer and internet and learned and efficient instructors whereas the least effective items considered were degree orientation, students’ apprenticeships in executive environments and education at the university. The findings showed a significant positive relation at the significance level of 0.05 between the level of education and age with the creation of entrepreneurial spirit amongst students. Furthermore a significant positive relation was observed between educational and academic factors, propensity to achievement, risk taking and ambiguity toleration, control source and family with entrepreneurial spirit at the significance level of 0.01. Also the results of multiple regression analysis showed that 79.7%of entrepreneurial spirit (the dependent variable) variance was explained by such independent variables as educational and academic factors, achievement propensity, being innovative, risk taking and ambiguity toleration, control source and family.
本文旨在探讨德黑兰大学农业与自然资源学院和Shahid Rajaiee教师培训大学两所大学木材与造纸行业学生创业精神的影响因素。使用科克伦公式从572名学生的目标人群中选出100名学生。受访者被要求填写一份标准化的问卷。结果表明,从学生的角度来看,动机、远见和成就倾向对被调查者的创业精神影响最大,而内部控制、例外能力和有系统的态度对被调查者的创业精神影响最小。此外,教育和学术因素对创业精神的影响,最有效的因素分别是计算机和互联网的良好接入和博学高效的导师,而最无效的因素是学位取向、学生在行政环境中的学徒和大学教育。研究结果显示,教育程度和年龄与学生创业精神的形成呈显著正相关,显著性水平为0.05。教育与学术因素、成就倾向、风险承担与模糊容忍、控制来源、家庭与创业精神之间存在显著正相关,显著水平为0.01。多元回归分析结果显示,79.7%的创业精神(因变量)方差可以被教育和学术因素、成就倾向、创新、风险承担和模糊容忍、控制来源和家庭等自变量解释。
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引用次数: 4
Effects of using radiation processing in nutrition science and their restriction: a review 辐射加工在营养科学中的应用及其限制
Pub Date : 2019-01-01 DOI: 10.26655/IJABBR.2019.1.3
M. Zarei
Isotopic and nuclear techniques play an important role in food and agriculture, health and industry. In the agricultural sciences and food technology sectors, recent research has elucidated the new potential application of radiation for microbial decontamination, food preservation, improve the nutritional quality of  foods  and effectively utilise its potential as human food or animal feed. The information presented will help researchers to identify techniques of relevance to them. Simplification of some of these techniques must be addressed so that they can be used more widely, especially in developing countries. This review suggest that a key challenge for the future is to use these techniques for improving efficiency of livestock production and decreasing environmental pollution.
同位素和核技术在粮食和农业、卫生和工业方面发挥着重要作用。在农业科学和食品技术领域,最近的研究已经阐明了辐射在微生物净化、食品保鲜、提高食品营养质量以及有效利用其作为人类食品或动物饲料的潜力方面的新的潜在应用。所提供的信息将有助于研究人员确定与他们相关的技术。必须设法简化其中一些技术,以便能够更广泛地使用,特别是在发展中国家。这一综述表明,未来的一个关键挑战是利用这些技术提高畜牧生产效率和减少环境污染。
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引用次数: 3
The Investigation Relation Between Inefficient Attitude and Social Undermining of the Employees of Football Clubs in Kerman 科尔曼足球俱乐部员工工作态度低下与社会破坏关系的调查
Pub Date : 2018-06-01 DOI: 10.18869/IJABBR.2018.76
Maryam Kafinejad
This research has been conducted by the aim of analysis of the relation of inefficient Attitude and social undermining of the employees of football clubs in Kerman. The research method is descriptive-correlative. The statistical population of this research includes all employees of teenage, youth and adult employees of Kerman city in 2017 with the total number of 192 from which 153 replied to the questionnaire. Collecting data from questionnaires, the inefficient Attitude with validity of 0.82 and stability of 0.93 and the social undermining questionnaire with the validity of 0.81 and stability of 0.87 were employed. The analysis of data was done by SPSS20 software, and some descriptive and inferential statics were used such as mean, variance, Pearson and Spearman correlative, and multi-variance linear regression. The result of the research illustrated that there is a direct and significant elation between inefficient Attitude and social undermining of the employees of football clubs in Kerman. Also, there is a direct and significant relation between inefficient Attitude about the performance and inefficient Attitude about social confirmation and social undermining of the employees of football clubs in Kerman. The best predicator of social undermining of employees can be the inefficient Attitude about social confirmation.
本研究旨在分析克尔曼足球俱乐部员工的低效态度与社会破坏的关系。研究方法是描述性相关的。本研究的统计人群包括2017年克尔曼市所有的青少年、青年和成年雇员,共192人,其中153人回答了问卷。从问卷中收集数据,采用效度为0.82、稳定性为0.93的“效率态度”问卷和效度为0.81、稳定性为0.87的“社会破坏”问卷。数据分析采用SPSS20软件,采用均值、方差、Pearson和Spearman相关及多方差线性回归等描述性和推理性统计方法。研究结果表明,克尔曼足球俱乐部员工的效率低下态度与社会破坏之间存在着直接而显著的相关关系。克尔曼足球俱乐部员工的绩效无效率态度与社会确认、社会破坏的无效率态度之间存在着显著的直接关系。员工社会破坏的最佳预测因子是对社会确认的低效态度。
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引用次数: 0
期刊
International journal of Advanced Biological and Biomedical Research
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