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Bioorganic investigation of encapsulated Cysteine derivative into polymeric nanocarrier 半胱氨酸衍生物包封聚合物纳米载体的生物有机研究
Pub Date : 2019-10-01 DOI: 10.33945/SAMI/IJABBR.2019.4.6
Ehab M. M. Ali, A. Hamed
In this work, the copolymer-based synthesized Cysteine-loaded nanocarriers prepared by a routine protocol, coprecipitation method. It is the first report to investigate the neuroprotective potential and biocompatibility of Cysteine derivatives loaded into poly(ethylene glycol)-block-poly(e−caprolactone) methyl ether (PEG-b-PCL). The average size of the polymeric/empty NCs was 89 nm and for polymeric/Synthesized derivative of Cysteine was 126 nm. The Drug Loading efficiency was 81%. The concentration of Polymeric NCs was 2.1 x 10 10 particles/ml and the zeta potential of polymeric/empty and polymeric/ Synthesized derivative of Cysteine NCs -5 mV and -11 mV respectively. Biological part of this work were investigated in the SH-SY5Y human neuroblastoma cell line using cell viability and toxicity assays. The concentration of polymeric NCs below 1 x 10 10 particles/ml described as a zero-point damageable for the cell line. Also the Synthesized derivative of Cysteine encapsulated into polymeric NCs have more neuroprotective effect compared to free Cysteine at lower concentration, and therefore, have a significant neuroprotective potential against Z-VAD-fmk and St-evoked SH-SY5Y cell damage.
本论文采用共沉淀法合成了基于共聚物的半胱氨酸负载纳米载体。这是第一个研究半胱氨酸衍生物负载到聚乙二醇-嵌段聚(e -己内酯)甲基醚(PEG-b-PCL)中的神经保护潜力和生物相容性的报告。聚合物/空NCs的平均尺寸为89 nm,聚合物/半胱氨酸合成衍生物的平均尺寸为126 nm。载药效率为81%。聚合物NCs的浓度为2.1 × 10 10粒/ml,聚合物/空半胱氨酸NCs和聚合物/合成半胱氨酸NCs衍生物的ζ电位分别为-5 mV和-11 mV。本研究的生物学部分在SH-SY5Y人神经母细胞瘤细胞系中进行了细胞活力和毒性试验。聚合物nc的浓度低于1 × 1010颗粒/ml,称为细胞系的零点损伤。与低浓度的游离半胱氨酸相比,聚合NCs包封的半胱氨酸衍生物具有更强的神经保护作用,因此对Z-VAD-fmk和st诱发的SH-SY5Y细胞损伤具有显著的神经保护潜力。
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引用次数: 1
Effect of sub-effective dose of GABA agonists on attenuation of morphine tolerance in rats: Behavioral and electrophysiological studies 亚有效剂量GABA激动剂对大鼠吗啡耐受减弱的影响:行为学和电生理研究
Pub Date : 2019-10-01 DOI: 10.33945/sami/ijabbr.2019.4.4
H. Manaheji, S. Mehrabadi
GABAergic drugs can change analgesic effect of morphine. Wide dynamic range (WDR) neurons play an important role in pain transmission and may change behaviors in morphine tolerance. In this study WDR neuron behaviors in morphine tolerant rats and rats treated with GABA agonists, were recorded to elucidate the effect of morphine and GABA agonists on WDR behavioral changes. Rats were divided to 4 groups: 1. Control, 2. Morphine tolerance (MT), 3. MT+ muscimol, 4- MT+ baclofen. To induce morphine tolerance in rats, they received morphine sulfate 10 mg/kg intraperitoneally for 8 days. In treatment group, GABA agonists were injected on days 1, 3, 5 and 8 before injection of morphine. To confirm morphine tolerance induced, formalin test was used. Extracellular single unit recording was used to record spinal WDR neurons. Results showed that chronic administration of morphine failed to attenuate formalin pain but GABA agonists improved analgesic effect of morphine.
gaba能药物能改变吗啡的镇痛作用。宽动态范围(WDR)神经元在疼痛传递中起重要作用,并可能改变吗啡耐受行为。本研究记录吗啡耐受大鼠和GABA激动剂治疗大鼠的WDR神经元行为,以阐明吗啡和GABA激动剂对WDR行为改变的影响。将大鼠分为4组:1;控制,2。吗啡耐受性(MT);MT+ muscimol, 4- MT+巴氯芬。大鼠腹腔注射硫酸吗啡10 mg/kg,连续8天诱导吗啡耐受。治疗组分别于吗啡注射前第1、3、5、8天注射GABA激动剂。采用福尔马林试验证实吗啡耐受。采用细胞外单单元记录法记录脊髓WDR神经元。结果表明,长期给药吗啡不能减轻福尔马林疼痛,但GABA激动剂能改善吗啡的镇痛效果。
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引用次数: 2
Heamatology, Serum Biochemistry, Relative Organ Weight and Bacteria Count of Broiler Chicken Given Different Levels of Luffa Aegyptiaca Leaf Extracts 饲喂不同水平埃及丝瓜叶提取物对肉鸡血液学、血清生化、相对脏器重量和细菌数量的影响
Pub Date : 2019-10-01 DOI: 10.33945/SAMI/IJABBR.2019.4.8
A. John
The objective of this study was to evaluate the heamato-biochemical, relative organ weight and bacteria count of broiler chicken given different levels of Luffa aegyptiaca leaf extract (LUF). A total of 250 day old Ross 308 broiler chicks of mixed sex were divided into five treatments, each group was further divided into five replicates each of ten (10) birds in a completely randomized design. Birds in treatment A were given 1.20g/litre of Neomycin in water, treatment B, C, D and E were given 5, 10, 15 and 20 ml/litre of LUF. Clean feed and water was given ad libitum and the experiment lasted for 3 weeks. The data obtained was used to evaluate the haematological parameters (PCV, RBC, Hb, WBC, MCV, MCH and MCHC), serum biochemical indices (Albumin, globulin, calcium, phosphorus, SGPT and SGOT), organ weight (liver, kidney, spleen, heart, lungs, pancreas and small intestine) and bacteria count (E.coli and Lactobacillus). Result obtained revealed that there was no significant differences (p>0.05) in the haematological, relative organ weight, serum biochemical parameters and E.coli count obtained. However, there was a significant difference (p
本试验旨在研究饲喂不同水平埃及丝瓜叶提取物(LUF)对肉鸡血液生化、相对脏器重量和细菌数量的影响。试验选用250日龄罗斯308混合性肉鸡,随机分为5个处理,每组再分为5个重复,每个重复10只鸡。A组给予1.20g/l的水中新霉素,B、C、D、E组给予5、10、15、20 ml/l的LUF。饲喂适量的清洁饲料和水,试验期3周。测定血液学指标(PCV、RBC、Hb、WBC、MCV、MCH和MCHC),血清生化指标(白蛋白、球蛋白、钙、磷、SGPT和SGOT),脏器重量(肝、肾、脾、心、肺、胰腺和小肠)和细菌数量(大肠杆菌和乳酸菌)。结果显示,两组血液学指标、相对脏器重量、血清生化指标及大肠杆菌计数均无显著差异(p>0.05)。两组间差异有统计学意义(p
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引用次数: 10
The evaluation and comparison of thermo-physical, chemical and biological properties of palladium (II) complexes on binuclear diamine ligands with different anions using the DFT method 用DFT方法评价和比较不同阴离子双核二胺配体上钯(II)配合物的热物理、化学和生物性质
Pub Date : 2019-10-01 DOI: 10.33945/SAMI/IJABBR.2019.4.3
Mohammad Jahidul Islam, Md. Nuruzzaman Sarker, A. Kumer, Sunanda Paul
As cancer is the top killer diseases in the world, the scientists and researchers have been searching the new drugs and remedy methods. Most of the anticancer drugs are organic compounds which were approved by the FDA while metallodrugs are very rare. In the present time, some palladium and rhodium complexes are going to use as anticancer molecules. The palladium (II) complex has higher anticancer activity against different cancer cell that is why the different amine ligands are considered under theoretical study by the method of density functional theory (DFT) to make a new molecule. Some thermo-physical parameter was conducted such as free energy, entropy, dipole moment, binding energy, nuclear energy, electronics energy, the heat of formation. On the other hand, the chemical reactivity properties like occupied Molecular Orbital Highest (HOMO), Lowest Unoccupied Molecular Orbital (LUMO), HOMO-LUMO gap, ionization potential, electronegativity, hardness, softness and electron affinity, and biological properties like charge density, surface area grid, volume, LogP, polarizability, refractivity, molecular mass were calculated using the DFT method. To make comparative biological properties, different anions such as chloride, nitrate, hydroxide, carbonate and sulfate ions were used as homogeneous and heterogeneous adding.
由于癌症是世界头号杀手,科学家和研究人员一直在寻找新的药物和治疗方法。大多数抗癌药物是FDA批准的有机化合物,而金属药物非常罕见。目前,一些钯铑配合物正被用作抗癌分子。钯(II)配合物对不同的癌细胞具有较高的抗癌活性,这就是为什么用密度泛函理论(DFT)的方法对不同胺配体进行理论研究以合成新分子的原因。测量了自由能、熵、偶极矩、结合能、核能、电子能、生成热等热物性参数。另一方面,利用DFT方法计算了聚合物的化学反应性质,如已占据分子轨道最高(HOMO)、最低未占据分子轨道(LUMO)、HOMO-LUMO间隙、电离势、电负性、硬度、柔软度和电子亲和性,以及电荷密度、表面积网格、体积、LogP、极化率、折射率、分子质量等生物学性质。为了比较生物性能,采用氯化物、硝酸盐、氢氧化物、碳酸盐和硫酸盐等阴离子作为均相和非均相添加。
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引用次数: 6
Effect of Feeding Different Levels of Luffa aegyptiaca Extracts on the Growth Performance of Broiler Chicken Fed Corn-Soya Meal Diet 饲喂不同水平埃及丝瓜提取物对玉米-豆粕饲粮肉鸡生长性能的影响
Pub Date : 2019-10-01 DOI: 10.33945/SAMI/IJABBR.2019.4.1
A. John
This experiment was carried out to investigate the effect of feeding different levels of luffa aegyptiaca extracts (LAP) on the growth performance of broiler chicken fed corn-soya meal diet. The study was carried out between January to March, 2019. A total of two hundred day old broiler chicks of mixed sex (Ross 308) were allocated into four treatment groups, each group was further divided into five replicates each of ten (10) birds. The growth performance parameters measured are: initial body weight, final body weight, average weight gain, average feed intake, average water intake and mortality. Clean feed and water were provided ad libitum and the experiment lasted for 42 days. Treatment 1 contained 1.25 g/ litre of Oxyteracycline, treatment 2, 3 and 4 contained LAP at 10, 20 and 30ml/litre. There was a significant (p0.05) influenced by LAP and OXY. It was concluded that LAP could be orally administered up to 30 ml/litre to broiler chickens without any negative effect on the growth and health performance of the animal.
本试验旨在研究饲喂不同水平埃及丝瓜提取物(LAP)对玉米-豆粕型饲粮肉鸡生长性能的影响。该研究于2019年1月至3月进行。试验选用200日龄混合性肉鸡罗斯308,随机分为4个处理组,每组再分为5个重复,每个重复10只鸡。测定的生长性能参数为:初重、末重、平均增重、平均采食量、平均采水量和死亡率。随机提供干净饲料和水,试验期42 d。处理1含有1.25 g/升的氧他环素,处理2、3和4含有10、20和30ml/升的LAP。LAP和OXY对其影响显著(p0.05)。综上所述,肉仔鸡口服LAP可达30 ml/l,对肉鸡生长和健康性能无不良影响。
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引用次数: 1
Evaluation of Spent Engine Oil and other Natural Materials on the Emergence of Harvester Ants (Messor galla Forel) in North Eastern Nigeria 废机油和其他天然物质对尼日利亚东北部收获蚁(Messor galla Forel)出现的影响
Pub Date : 2019-10-01 DOI: 10.33945/SAMI/IJABBR.2019.4.5
E. Okrikata, C. E. Anaso, S. Bukar
Harvester ants (Messor galla Forel) defied various control strategies. Two field experiments were conducted to evaluate the effects of Spent Engine-Oil and other nature-based materials on their emergence in Maiduguri, Borno State of Nigeria. The treatments evaluated are spot application of spent engine-oil and some natural materials in experiment 1 and individual and equal mixture of Pure Neem Seed Kernel oil and Spent Engine-oil in experiment 2. Spot application of permethrin served as control. Experiment 1 results shows no significant difference (p > 0.05) between permethrin and spent engine-oil (which was the most effective treatment). While neem seed oil was significantly (p 0.05) in the population index of ants that emerged from nests treated with aqueous neem seed extract, salt/potash solution and extract of Eugenia aromatic fruit from those of the control. Experiment 2 result showed no significant difference (p 0.05) from the untreated control. While the result shows no significant difference between the treatments, it was observed that equal mixture of Spent Engine-oil and Neem Seed Kernel Oil was most effective, followed by Spent Engine-Oil, Permethrin and Neem Seed Kernel Oil, in that order.
收获蚁(Messor galla Forel)违抗各种控制策略。在尼日利亚博尔诺州迈杜古里进行了两次实地试验,以评估废机油和其他自然材料对其出现的影响。试验1采用废机油和一些天然材料进行现场处理,试验2采用纯印楝籽油和废机油单独均匀混合处理。现场施用氯菊酯作为对照。试验1结果表明,氯菊酯与废机油处理效果无显著差异(p > 0.05)。而印楝籽油对浸提液、盐/钾溶液和金针叶提取物处理蚁巢出巢的蚁群指数影响显著(p 0.05)。实验2结果与未处理对照组无显著差异(p 0.05)。结果表明,废发动机油与印楝仁油等量混合处理效果最佳,其次为废发动机油、氯菊酯和印楝仁油。
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引用次数: 1
Experimental Investigation of Turbidity Flow in 90 Degree Bend with Mobile Bed 90度弯曲流动床浊度流动的实验研究
Pub Date : 2019-10-01 DOI: 10.33945/SAMI/IJABBR.2019.4.7
M. Mohammadi, M. Ghomeshi
Turbidity currents in the ocean and lakes are driven by suspended sediment. The vertical profiles of velocity and excess density are shaped by interaction between the current and bed as well as between the current and the ambient water. This paper presents 48 series of experiments in which saline gravity currents flow through a laboratory sinuous flume. flume contains three successive bends with three different relative curvature radiuses: R/B=2, 4 and 6, 8.5m length, 20cm width and 70cm height. Experiments performed by four discharges (0.7, 1, 1.5, 2 lit/s) and four concentrations (10, 15, 20, 25 gr/lit). ADV was used to record the local velocity. According to the results of experiments on the mobile bed, increasing the concentration of the incoming flow, the flow velocity of the fluid is also increased and, the maximum velocity occurs near the bed instead of the top of the current. The important point in the flow rate profiles is that the rate of increase in velocity depends on changes in the form of the bed due to the increase in concentration. By increasing concentrations of turbidity flow, the shear stress of the bed is also increased. Therefore, the rate of increase in velocity will occur by removing the bed forms and reducing the roughness and shear stress of the bed. Thus, increasing the concentration increases the power of the current, so at the beginning, the roughness and shear stress of the bed increased and then by removal of bed forms shear stress decreased.
海洋和湖泊中的浑浊流是由悬浮沉积物驱动的。流速和超密度的垂直剖面是由水流与河床以及水流与周围水体的相互作用形成的。本文介绍了盐水重力流通过实验室弯曲水槽的48个系列实验。水槽包含三个连续的弯道,具有三种不同的相对曲率半径:R/B= 2,4和6,8.5 m长,20cm宽,70cm高。实验采用四种放电(0.7、1、1.5、2 lit/s)和四种浓度(10、15、20、25 gr/lit)进行。用ADV记录局部速度。在移动床上进行的实验结果表明,随着来流浓度的增加,流体的流速也随之增大,并且最大流速发生在床层附近,而不是流的顶部。在流速剖面中,重要的一点是流速的增加速率取决于由于浓度增加而引起的床层形式的变化。随着浊度流浓度的增加,床层的剪切应力也随之增加。因此,速度的增加速率将通过去除床层形式和降低床层的粗糙度和剪切应力来实现。因此,浓度的增加增加了电流的功率,因此在开始时,床层的粗糙度和剪应力增加,然后通过去除床层形式剪应力减小。
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引用次数: 0
Evaluation of Neem Dust Formulations for the Control of Sorghum Stemborers in the Semi-Arid Zone of Nigeria 尼日利亚半干旱区印楝粉防治高粱螟虫效果评价
Pub Date : 2019-10-01 DOI: 10.33945/SAMI/IJABBR.2019.4.2
E. Okrikata, C. E. Anaso
Sorghum stemborer species remains key yield limiting factor for sorghum production in the Nigerian semi-arid region. When they can afford, farmers rely almost solely on synthetic insecticides for control. Owing to the misuse and environmental and health challenges attributed to the use of synthetic insecticides, a two-year field study was carried out at the University of Maiduguri Research Farm to evaluate the efficacy of various neem dust formulations for the control of Sorghum stemborers. The experimental design was Randomized Complete Block Design and various neem dust formulations were compared with carbaryl dust and the control (untreated). All the insecticide treatments significantly (p < 0.05) reduced stalk and peduncle damage caused by stemborers with a resultant increase in grain yield when compared with the control. Neem Kernel Powder (NKP) + Finesand proved more superior in efficacy than all the other insecticide treatments in checking stemborer damage.
在尼日利亚半干旱区,高粱蒸虫品种仍然是限制高粱产量的关键因素。当农民负担得起时,他们几乎完全依靠合成杀虫剂来控制。由于滥用合成杀虫剂以及使用合成杀虫剂带来的环境和健康挑战,在迈杜古里大学研究农场进行了一项为期两年的实地研究,以评估各种印楝粉配方防治高粱螟虫的功效。实验设计采用随机完全区组设计,将不同印楝粉配方与西威因粉和对照(未处理)进行比较。与对照相比,各杀虫剂处理显著(p < 0.05)降低了茎螟虫对稻秆和稻梗的危害,提高了籽粒产量。印楝仁粉(NKP) + Finesand在防治蒸虫病方面的效果优于其他所有杀虫剂处理。
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引用次数: 1
Risk Factors of Gangrenous Cholecystitis in Patients with Acute Cholecystitis: A Cross-Sectional Study 急性胆囊炎患者坏疽性胆囊炎的危险因素:横断面研究
Pub Date : 2019-09-01 DOI: 10.33945/SAMI/IJABBR.2019.3.7
M. Roozbahani, M. K. Shahmoradi, J. Mehri, Arefe Qolampoor, B. Nasiri, Fatemeh Pakmehr
Introduction: The aim of this study was to determine factors affecting the development of acute cholecystitis toward gangrenous cholecystitis in an Iranian society.Materials and Methods: In this cross-sectional study, all patients who underwent cholecystectomy in the educational hospitals of Khorramabad from August 2013 to August 2016 were included in the study regardless of their age range. The data collected in each patient included demographic data, underlying illnesses, positive history and physical examination prior to surgery, early laboratory findings and preoperative imaging findings. Finally, patients were divided into two groups of acute Cholecystitis and Gangrenous Cholecystitis based on pathological findings after cholecystectomy. The data was finally analyzed using SPSS software.Results: 51 patients were examined during the study. Histological studies indicated the presence of microscopic evidence of acute cholecystitis in all patients, of which 19 had histological evidence of Gangrenous Cholecystitis. In this study, nine variables that could be effective on the prognosis of acute cholecystitis and its progression toward the Gangrenous Cholecystitis were identified. These variables included age, WBC count, diabetes mellitus and coronary artery disease, fluid accumulation around gallbladder, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, and lipase. Finally, multivariate analysis was performed using logistic regression. It was found that the age of ≥51 was effective in the development of diabetes mellitus.Conclusion: In this study, it was observed that high age, diabetes mellitus and leukocytosis could lead to the development of acute cholecystitis toward Gangrenous Cholecystitis, a finding that requires more extensive studies with more sample size.
简介:本研究的目的是确定影响伊朗社会急性胆囊炎向坏疽性胆囊炎发展的因素。材料与方法:本横断面研究纳入2013年8月至2016年8月在霍拉马巴德教育医院行胆囊切除术的所有患者,不论其年龄范围。每位患者收集的数据包括人口统计数据、基础疾病、手术前的阳性病史和体格检查、早期实验室检查结果和术前影像学检查结果。最后根据胆囊切除术后的病理表现将患者分为急性胆囊炎和坏疽性胆囊炎两组。最后用SPSS软件对数据进行分析。结果:51例患者在研究期间接受了检查。组织学检查显示所有患者均有急性胆囊炎的显微证据,其中19例患者有坏疽性胆囊炎的组织学证据。本研究确定了影响急性胆囊炎预后及其向坏疽性胆囊炎发展的9个变量。这些变量包括年龄、白细胞计数、糖尿病和冠状动脉疾病、胆囊周围积液、天冬氨酸转氨酶、丙氨酸转氨酶、碱性磷酸酶和脂肪酶。最后,采用logistic回归进行多变量分析。发现年龄≥51岁对糖尿病的发生有效。结论:本研究发现,高龄、糖尿病和白细胞增多可导致急性胆囊炎向坏疽性胆囊炎发展,这一发现需要更广泛、更大样本量的研究。
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引用次数: 1
Factors Affecting the Use of Sustainable Agriculture in Iran's Potato Cultivars 影响伊朗马铃薯品种可持续农业利用的因素
Pub Date : 2019-07-01 DOI: 10.33945/SAMI/IJABBR.2019.3.3
Tahmineh Kashani, M. O. Najafabadi, F. Lashgarara
Current research examines the economic, social, cultural, policy, educational and promotional factors in using sustainable agriculture operations. Research is an applied type and its method is correlation. The statistical population consisted of 102 members of Iran Potato Cultivars. Data were collected using a census method and a questionnaire was used. The Cronbach's alpha coefficient was obtained among 0.4723.0 ± 0.946 / 0, indicating the reliability of this tool. Statistical analysis of data was performed using SPSS20 software. The results of regression analysis indicated that three variables: economic factors, policy factors, educational and promotional factors were the most important independent variables affecting the dependent variable of sustainable agriculture, that was 42.1% of the variation of dependent variable (implementation of sustainable agricultural activity) that explain the research.Also the results indicated that the economic factor variable with the beta coefficient equal to 0.339 is the most important variable affecting the implementation of sustainable agricultural practices. The variable policy making factor with a regression coefficient of 0.146 is the least important factor affecting the implementation of sustainable agricultural operations
目前的研究考察了利用可持续农业经营的经济、社会、文化、政策、教育和促进因素。研究是一种应用性研究,研究方法是相关性研究。统计群体由102个伊朗马铃薯品种成员组成。采用人口普查法和问卷调查法收集数据。Cronbach’s alpha系数在0.4723.0±0.946 / 0之间,表明该工具的可靠性。采用SPSS20软件对数据进行统计分析。回归分析结果表明,经济因素、政策因素、教育和推广因素是影响可持续农业因变量的最重要的自变量,占可持续农业活动实施因变量变异的42.1%。经济因素是影响可持续农业实践实施的最重要变量,其贝塔系数为0.339。变量政策制定因子的回归系数为0.146,是影响农业可持续经营实施的最不重要因素
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引用次数: 0
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International journal of Advanced Biological and Biomedical Research
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