Pub Date : 2023-10-15DOI: 10.18231/j.ijcbr.2023.034
None Vanitha S, Victoria Job, None Aleyamma T K
Unexplained infertility is a perplexing disorder. 30% of infertile couples are diagnosed with unexplained infertility with all standard fertility investigations normal. Oxidative stress has been shown to have a negative impact on reproductive function in women with endometriosis and (PCOS) polycystic ovarian syndrome. In another study antioxidant supplementation improved OS induced infertility. Many studies have demonstrated an increase in Malondialdehyde in serum of women with infertility and vitamin E as a protective antioxidant in the body with positive effect on the fertility. There is growing evidence linking OS and unexplained infertility. Based on this knowledge the specific group of unexplained infertile women were chosen to assess their levels of serum antioxidant enzymes, Vitamin E and lipid peroxidation marker. To compare the Levels of Malondialdehyde, Antioxidant enzymes and Vitamin E in the serum of women with unexplained infertility and control group. : Case control study. 70 normal ovulatory women who conceived within 12 months of contraceptive free intercourse, and with no history of miscarriage were recruited in the control group. 70 women with unexplained infertility were recruited as study group. All participants included in the study were between 28 and 38 years of age. Serum levels of MDA, Antioxidant enzymes (GST, SOD, Catalase) and Vitamin E concentrations were compared between two groups. Vitamin E concentrations were determined by using High performance liquid chromatography. Antioxidant enzymes and MDA were measured by standard spectrophotometric assay. : Data was analysed using SPSS Software. Continuous parameters were analysed using Mann Whitney U test. There is a significant decrease in the vitamin E levels 3.80 ug/ml) in the unexplained infertile group as compared to controls 6.0 ug/ml). All women in the study group showed significantly (P<0.05) higher levels of MDA and lower levels of antioxidant enzymes such as GST, SOD and Catalase as compared to the fertile women. This decrease was found to be significant with p value of <0.05. The study group have a higher oxidative stress status and low level of antioxidants compared to control group. Serial measurement of oxidative stress biomarkers and their defense system may help to understand the aetiology of unexplained infertility and to enhance their chances of conception.
{"title":"Evaluation of vitamin E levels, antioxidant enzymes and malondialdehyde in women with unexplained infertility","authors":"None Vanitha S, Victoria Job, None Aleyamma T K","doi":"10.18231/j.ijcbr.2023.034","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.034","url":null,"abstract":"Unexplained infertility is a perplexing disorder. 30% of infertile couples are diagnosed with unexplained infertility with all standard fertility investigations normal. Oxidative stress has been shown to have a negative impact on reproductive function in women with endometriosis and (PCOS) polycystic ovarian syndrome. In another study antioxidant supplementation improved OS induced infertility. Many studies have demonstrated an increase in Malondialdehyde in serum of women with infertility and vitamin E as a protective antioxidant in the body with positive effect on the fertility. There is growing evidence linking OS and unexplained infertility. Based on this knowledge the specific group of unexplained infertile women were chosen to assess their levels of serum antioxidant enzymes, Vitamin E and lipid peroxidation marker. To compare the Levels of Malondialdehyde, Antioxidant enzymes and Vitamin E in the serum of women with unexplained infertility and control group. : Case control study. 70 normal ovulatory women who conceived within 12 months of contraceptive free intercourse, and with no history of miscarriage were recruited in the control group. 70 women with unexplained infertility were recruited as study group. All participants included in the study were between 28 and 38 years of age. Serum levels of MDA, Antioxidant enzymes (GST, SOD, Catalase) and Vitamin E concentrations were compared between two groups. Vitamin E concentrations were determined by using High performance liquid chromatography. Antioxidant enzymes and MDA were measured by standard spectrophotometric assay. : Data was analysed using SPSS Software. Continuous parameters were analysed using Mann Whitney U test. There is a significant decrease in the vitamin E levels 3.80 ug/ml) in the unexplained infertile group as compared to controls 6.0 ug/ml). All women in the study group showed significantly (P&#60;0.05) higher levels of MDA and lower levels of antioxidant enzymes such as GST, SOD and Catalase as compared to the fertile women. This decrease was found to be significant with p value of &#60;0.05. The study group have a higher oxidative stress status and low level of antioxidants compared to control group. Serial measurement of oxidative stress biomarkers and their defense system may help to understand the aetiology of unexplained infertility and to enhance their chances of conception.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"72 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135760904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.18231/j.ijcbr.2023.040
Manik Sharma, Manish Soni, Amita Gupta
The present study was conducted toexamine the Hepatoprotective activity against the carbon tetrachloride toxicity in Wister rats. The Petroleum ether, ethyl acetate and methanol extract of was prepared and evaluated for phytochemical screening. The serum level of glutamic oxaloacetate transaminase (SGOT) Glutomicpyruvate transaminase (SGPT) and bilirubin were investigated for the assessment of hepatoprotective activity of ethyl acetate of extract . Additionally, the histological changes in liver were studied. The primary phytochemical investigation of the extract of revealed the presence of Flavonoids, tannins, and carbohydrates. Pretreatment with ethyl acetate extract of showed a significant (P<0.05) decrease in serum SGOT, SGPT and bilirubin when compared to control group rats treated with CCl in dose dependent manner. The outcomes of histology study revealed that there was significant reversal of histological functions of liver. In conclusion, the findings of study validated that the can improve CCl–induced hepatic toxicity.
{"title":"Protective effect of Ageratum conyzoides against carbon tetrachloride –induced hepatic damage in rats","authors":"Manik Sharma, Manish Soni, Amita Gupta","doi":"10.18231/j.ijcbr.2023.040","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.040","url":null,"abstract":"The present study was conducted toexamine the Hepatoprotective activity against the carbon tetrachloride toxicity in Wister rats. The Petroleum ether, ethyl acetate and methanol extract of was prepared and evaluated for phytochemical screening. The serum level of glutamic oxaloacetate transaminase (SGOT) Glutomicpyruvate transaminase (SGPT) and bilirubin were investigated for the assessment of hepatoprotective activity of ethyl acetate of extract . Additionally, the histological changes in liver were studied. The primary phytochemical investigation of the extract of revealed the presence of Flavonoids, tannins, and carbohydrates. Pretreatment with ethyl acetate extract of showed a significant (P&#60;0.05) decrease in serum SGOT, SGPT and bilirubin when compared to control group rats treated with CCl in dose dependent manner. The outcomes of histology study revealed that there was significant reversal of histological functions of liver. In conclusion, the findings of study validated that the can improve CCl–induced hepatic toxicity.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135760383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.18231/j.ijcbr.2023.045
Abhinav Manish
Diabetes mellitus is a chronic metabolic disorder characterized by elevated blood glucose levels, resulting from either insufficient insulin production or the body's inability to effectively utilize insulin. This condition has become a global health concern, with an increasing prevalence worldwide. This review article aims to provide an overview of diabetes mellitus, its diagnostic criteria, and recent updates in management, complications, and a discussion on its implications for public health.
{"title":"Diabetes mellitus: A lifestyle disorder","authors":"Abhinav Manish","doi":"10.18231/j.ijcbr.2023.045","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.045","url":null,"abstract":"Diabetes mellitus is a chronic metabolic disorder characterized by elevated blood glucose levels, resulting from either insufficient insulin production or the body's inability to effectively utilize insulin. This condition has become a global health concern, with an increasing prevalence worldwide. This review article aims to provide an overview of diabetes mellitus, its diagnostic criteria, and recent updates in management, complications, and a discussion on its implications for public health.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"87 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135760377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-15DOI: 10.18231/j.ijcbr.2023.027
T. Saraladevi
When electrolyte imbalances are not recognized, they are known to cause severe morbidity and mortality. Dyselectrolytemia is frequently curable. Hyponatremia is a common symptom seen in the elderly. Laboratory equipment may be lacking in rural healthcare settings. As a result, the purpose of this study was to investigate the feasibility of developing a screening technique that can aid in the detection of hyponatremia in such facilities. Serum sodium levels in 120 samples were determined by direct, indirect, and colorimetric ISE. SPSS version 17, NCSS 11, and MINITAB 18 software were used for statistical analysis. Statistical significance was defined as a p-value of 0.05. While comparing the instruments, a basic linear regression analysis was performed, and a regression equation for sodium levels was established. The Bland-Altman analysis yielded a 95% agreement limit between the instruments, which is well within the CLIA-recommended target value of 4 mmol/L in the hyponatremic and normonatremic ranges. The resulting regression equation computes a predicted value for direct and indirect ISE using the colorimeter readings, making it similar to all three instruments in the hyponatremic and normonatremic ranges. The colorimetric method can be used as a low-cost screening technique to identify hyponatremic elderly people for whom a tertiary hospital may be inaccessible, allowing for early care.
当电解质失衡未被发现时，它们会导致严重的发病率和死亡率。电解质障碍通常是可以治愈的。低钠血症是老年人的常见症状。农村卫生保健机构可能缺乏实验室设备。因此，本研究的目的是研究开发一种筛查技术的可行性，该技术可以帮助在这些设施中检测低钠血症。120个样品的血清钠水平通过直接、间接和比色ISE测定。采用SPSS version 17、NCSS 11、MINITAB 18软件进行统计分析。统计学意义定义为p值为0.05。在比较仪器的同时，进行了基本的线性回归分析，建立了钠含量的回归方程。Bland-Altman分析得出仪器之间95%的一致性限制，这完全在clia推荐的低钠和正常钠范围内4 mmol/L的目标值之内。由此产生的回归方程使用色度计读数计算直接和间接ISE的预测值，使其与低钠和正钠范围内的所有三种仪器相似。比色法可作为一种低成本的筛查技术，用于识别无法进入三级医院的低钠血症老年人，以便进行早期护理。
{"title":"A comparison of serum sodium levels measured using a colorimetric kit approach and those determined using direct and indirect ion selective electrode techniques- in a Hospital Central Lab","authors":"T. Saraladevi","doi":"10.18231/j.ijcbr.2023.027","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.027","url":null,"abstract":"When electrolyte imbalances are not recognized, they are known to cause severe morbidity and mortality. Dyselectrolytemia is frequently curable. Hyponatremia is a common symptom seen in the elderly. Laboratory equipment may be lacking in rural healthcare settings. As a result, the purpose of this study was to investigate the feasibility of developing a screening technique that can aid in the detection of hyponatremia in such facilities. Serum sodium levels in 120 samples were determined by direct, indirect, and colorimetric ISE. SPSS version 17, NCSS 11, and MINITAB 18 software were used for statistical analysis. Statistical significance was defined as a p-value of 0.05. While comparing the instruments, a basic linear regression analysis was performed, and a regression equation for sodium levels was established. The Bland-Altman analysis yielded a 95% agreement limit between the instruments, which is well within the CLIA-recommended target value of 4 mmol/L in the hyponatremic and normonatremic ranges. The resulting regression equation computes a predicted value for direct and indirect ISE using the colorimeter readings, making it similar to all three instruments in the hyponatremic and normonatremic ranges. The colorimetric method can be used as a low-cost screening technique to identify hyponatremic elderly people for whom a tertiary hospital may be inaccessible, allowing for early care.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"76 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76833186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-15DOI: 10.18231/j.ijcbr.2023.028
M. Coulibaly, Adama Kondé, D. Traoré, O. Bah, Valentin Sagara, B. Maiga
Serum AFP as a poor clinical performance values especially when it comes to deal with the early and AFP-negative diagnostic of HCC. The aim of this work was to assess the contribution of AFP in the diagnosis of HCC. : A total of 95 subjects was enrolled a prospective observational study by consecutive enrolment and divided in two groups. The first group was made up with subjects in whom the diagnosis of HCC had been retained while the second was the control group which was free of HCC. AFP levels were performed by electrochemiluminescence immunoassay on the cobas e411. Data were captured in Excel and analyzed by Ri386 version 4.1.2 binary for macOS 10.13. The Log of median of AFP in HCC subjects was significantly greater than in non HCC subjects 6.91 ng/mL versus 1.43 ng/mL, Wilcoxon < 0.001. At the cut-off of 200 ng/mL, the clinical performances showed an acceptable sensitivity 97.1% CI 95% [93.7 – 100] but a poor specificity 73,8% CI 95% [64.9 – 82.6] and out of the 34 cases of HCC, one case (2.9%) was AFP-negative HCC. our data show an acceptable sensitivity but a weak specificity of AFP as a biomarker for HCC at cut-off 200 ng/mL. This suggests that AFP should be used with other biomarkers mainly for the early and AFP-negative HCC diagnosis.
血清AFP作为一项较差的临床表现价值，尤其在处理早期及AFP阴性的HCC诊断时。本研究的目的是评估AFP在HCC诊断中的作用。采用连续入组的方法，将95名受试者分为两组，纳入前瞻性观察性研究。第一组由保留HCC诊断的受试者组成，第二组是没有HCC的对照组。用电化学发光免疫法测定cobas e411的AFP水平。数据在Excel中捕获，并在macOS 10.13上使用Ri386 4.1.2二进制文件进行分析。HCC组甲胎蛋白中位对数显著大于非HCC组，前者为6.91 ng/mL，后者为1.43 ng/mL, Wilcoxon < 0.001。在200 ng/mL的临界值下，临床表现为可接受的敏感性为97.1% CI 95%[93.7 - 100]，但特异性较差73,8% CI 95%[64.9 - 82.6]， 34例HCC中有1例(2.9%)为afp阴性HCC。我们的数据显示AFP作为HCC的生物标志物具有可接受的敏感性，但特异性较弱，临界值为200 ng/mL。这表明AFP应与其他生物标志物联合使用，主要用于早期和AFP阴性的HCC诊断。
{"title":"Assessment of alpha-fetoprotein clinical performances in the diagnosis of the hepatocellular carcinoma at Sominé DOLO hospital of Mopti","authors":"M. Coulibaly, Adama Kondé, D. Traoré, O. Bah, Valentin Sagara, B. Maiga","doi":"10.18231/j.ijcbr.2023.028","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.028","url":null,"abstract":"Serum AFP as a poor clinical performance values especially when it comes to deal with the early and AFP-negative diagnostic of HCC. The aim of this work was to assess the contribution of AFP in the diagnosis of HCC. : A total of 95 subjects was enrolled a prospective observational study by consecutive enrolment and divided in two groups. The first group was made up with subjects in whom the diagnosis of HCC had been retained while the second was the control group which was free of HCC. AFP levels were performed by electrochemiluminescence immunoassay on the cobas e411. Data were captured in Excel and analyzed by Ri386 version 4.1.2 binary for macOS 10.13. The Log of median of AFP in HCC subjects was significantly greater than in non HCC subjects 6.91 ng/mL versus 1.43 ng/mL, Wilcoxon < 0.001. At the cut-off of 200 ng/mL, the clinical performances showed an acceptable sensitivity 97.1% CI 95% [93.7 – 100] but a poor specificity 73,8% CI 95% [64.9 – 82.6] and out of the 34 cases of HCC, one case (2.9%) was AFP-negative HCC. our data show an acceptable sensitivity but a weak specificity of AFP as a biomarker for HCC at cut-off 200 ng/mL. This suggests that AFP should be used with other biomarkers mainly for the early and AFP-negative HCC diagnosis.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72889852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-15DOI: 10.18231/j.ijcbr.2023.030
Poornima R Varma
: Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer occurs in people with liver diseases like chronic hepatitis B and hepatitis C virus. Early diagnosis and monitoring of HCC is possible with the help of tumor marker AFP. The aim of the study was to assess whether AFP level is associated with selected liver function tests in hepatocellular carcinoma patients.: Total 90 individuals are selected, 50 HCC patients with AFP >20ng/ml and 40 subjects (normal LFT and AFP value <10ng/ml) with an age between 40-70. Serum is used for analysis of AFP, SGOT, SGPT, ALP by Maglumi 1000 and Siemen biochemistry analyzer (using principle Chemiluminescence immunoassay) respectively. Unpaired T test (IBM SPSS 22.0) were used. Elevated levels of AFP (p value <0.05), SGOT (p value < 0.001), SGPT (p value < 0.001), ALP (p value < 0.001) shows statistical significance within study population and also indicated the risk of developing HCC with respect to AFP level in liver disease patients with elevated LFT parameters.: From the study, it is concluded that LFT and AFP are comparatively elevated in HCC. Treatment is usually not possible with advanced HCC due to failure of not doing proper screening at appropriate time. Early diagnosis and monitoring of HCC are possible with the help of tumor marker AFP.
{"title":"Association between serum alpha-fetoproteinlevel and liver function parameters in hepatocellular carcinoma patients: A case-control study","authors":"Poornima R Varma","doi":"10.18231/j.ijcbr.2023.030","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.030","url":null,"abstract":": Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer occurs in people with liver diseases like chronic hepatitis B and hepatitis C virus. Early diagnosis and monitoring of HCC is possible with the help of tumor marker AFP. The aim of the study was to assess whether AFP level is associated with selected liver function tests in hepatocellular carcinoma patients.: Total 90 individuals are selected, 50 HCC patients with AFP >20ng/ml and 40 subjects (normal LFT and AFP value <10ng/ml) with an age between 40-70. Serum is used for analysis of AFP, SGOT, SGPT, ALP by Maglumi 1000 and Siemen biochemistry analyzer (using principle Chemiluminescence immunoassay) respectively. Unpaired T test (IBM SPSS 22.0) were used. Elevated levels of AFP (p value <0.05), SGOT (p value < 0.001), SGPT (p value < 0.001), ALP (p value < 0.001) shows statistical significance within study population and also indicated the risk of developing HCC with respect to AFP level in liver disease patients with elevated LFT parameters.: From the study, it is concluded that LFT and AFP are comparatively elevated in HCC. Treatment is usually not possible with advanced HCC due to failure of not doing proper screening at appropriate time. Early diagnosis and monitoring of HCC are possible with the help of tumor marker AFP.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77995154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-15DOI: 10.18231/j.ijcbr.2023.016
Wilma Delphine Silvia CR, Akshay S Atre, Preksha Shivakumar, A. Chakraborty
Mucopolysaccharidosis is a lysosomal storage disorder, caused due to deficiency of enzymes required for the breakdown of Mucopolysaccharides. These undegraded Mucopolysaccharides accumulate in various tissues and cause characteristic features like neurological deficit, impaired motor function, developmental delay, hearing loss, behavioral problems, corneal clouding, glaucoma, respiratory distress, coarse facial features, skeletal deformities, and organomegaly. Based on deficient enzymes they have divided into subtypes Mucopolysaccharidosis I (MPS I) Hunter syndrome (I H / I HS / I S), Mucopolysaccharidosis II(MPS II) Hunter syndrome (severe and mild form), Mucopolysaccharidosis III (MPS III) Sanfilippo syndrome, Mucopolysaccharidosis IV(MPS IV) Morquio syndrome, Mucopolysaccharidosis VI(MPS VI) Maroteaux Lamy syndrome, Mucopolysaccharidosis VI (MPS VII) Sly syndrome. Diagnosis is classically based on clinical examination and urine analysis. Enzyme assay can also aid in diagnosis. Chorionic villi sampling and amniocentesis are also becoming popular. The main objective of treatment is to improve the quality of life. Symptomatic management includes daily exercise, physiotherapy, tonsillectomy, shunting surgery, and corneal transplantation. There are various recent concepts utilized for the treatment of Mucopolysaccharidosis. This review article emphasizes such treatment aspects as Hematopoietic stem cell therapy, Enzyme replacement therapy, Gene therapy, Nano-enabled therapy, and Substrate reduction therapy.
粘多糖病是一种溶酶体储存障碍，由于缺乏分解粘多糖所需的酶而引起。这些未降解的粘多糖在各种组织中积累，导致神经功能障碍、运动功能受损、发育迟缓、听力丧失、行为问题、角膜混浊、青光眼、呼吸窘迫、面部粗糙、骨骼畸形和器官肿大等特征性特征。根据酶缺陷，将其分为粘多糖病I(MPS I) Hunter综合征(I H / I HS / I S)、粘多糖病II(MPS II) Hunter综合征(重度和轻度)、粘多糖病III (MPS III) Sanfilippo综合征、粘多糖病IV(MPS IV) Morquio综合征、粘多糖病VI(MPS VI) Maroteaux Lamy综合征、粘多糖病VI(MPS VII) Sly综合征。诊断通常基于临床检查和尿液分析。酶测定也有助于诊断。绒毛膜绒毛取样和羊膜穿刺术也越来越流行。治疗的主要目的是改善生活质量。症状处理包括日常运动、物理治疗、扁桃体切除术、分流手术和角膜移植。有各种最近的概念用于治疗粘多糖病。本文主要从造血干细胞治疗、酶替代治疗、基因治疗、纳米治疗、底物还原治疗等方面进行综述。
{"title":"Mucopolysaccharidosis: An overview and new treatment modalities","authors":"Wilma Delphine Silvia CR, Akshay S Atre, Preksha Shivakumar, A. Chakraborty","doi":"10.18231/j.ijcbr.2023.016","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.016","url":null,"abstract":"Mucopolysaccharidosis is a lysosomal storage disorder, caused due to deficiency of enzymes required for the breakdown of Mucopolysaccharides. These undegraded Mucopolysaccharides accumulate in various tissues and cause characteristic features like neurological deficit, impaired motor function, developmental delay, hearing loss, behavioral problems, corneal clouding, glaucoma, respiratory distress, coarse facial features, skeletal deformities, and organomegaly. Based on deficient enzymes they have divided into subtypes Mucopolysaccharidosis I (MPS I) Hunter syndrome (I H / I HS / I S), Mucopolysaccharidosis II(MPS II) Hunter syndrome (severe and mild form), Mucopolysaccharidosis III (MPS III) Sanfilippo syndrome, Mucopolysaccharidosis IV(MPS IV) Morquio syndrome, Mucopolysaccharidosis VI(MPS VI) Maroteaux Lamy syndrome, Mucopolysaccharidosis VI (MPS VII) Sly syndrome. Diagnosis is classically based on clinical examination and urine analysis. Enzyme assay can also aid in diagnosis. Chorionic villi sampling and amniocentesis are also becoming popular. The main objective of treatment is to improve the quality of life. Symptomatic management includes daily exercise, physiotherapy, tonsillectomy, shunting surgery, and corneal transplantation. There are various recent concepts utilized for the treatment of Mucopolysaccharidosis. This review article emphasizes such treatment aspects as Hematopoietic stem cell therapy, Enzyme replacement therapy, Gene therapy, Nano-enabled therapy, and Substrate reduction therapy.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86614069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-15DOI: 10.18231/j.ijcbr.2023.020
G. Gulavani, S. Panchbudhe, S. Gawade, Farheen B Mujawar
HbA1c is one of the important laboratory markers of blood glucose levels monitoring over last 2-3 months. Studies have shown that many factors affect HbA1c levels, hence it is not the only investigation to diagnose diabetes mellitus. As both iron deficiency anaemia and diabetes mellitus are highly prevalent disorders, effect of iron deficiency anaemia on HbA1C need to be evaluated. Many factors such as haemolytic anaemia, vitamin deficiencies, pregnancy. Kidney disease, haemoglobin variants can affect HbA1C levels. This study had two groups of participants. Group 1 included diabetic patients with iron deficiency anaemia and group 2 included diabetic patients without iron deficiency anaemia. Significant correlation between HbA1C and iron deficiency anaemia has been found. PCV, MCH, MCHC, MCV are low in diabetics with Iron deficiency anaemia and high in diabetics without iron deficiency anaemia. Need for anaemia screening before treatment planning of diabetes based on HBA1C levels must be considered and for optimal diabetes control, treating anemia is necessary.
{"title":"Impact of anemia on HbAC level in type 2 diabetes mellitus patients","authors":"G. Gulavani, S. Panchbudhe, S. Gawade, Farheen B Mujawar","doi":"10.18231/j.ijcbr.2023.020","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.020","url":null,"abstract":"HbA1c is one of the important laboratory markers of blood glucose levels monitoring over last 2-3 months. Studies have shown that many factors affect HbA1c levels, hence it is not the only investigation to diagnose diabetes mellitus. As both iron deficiency anaemia and diabetes mellitus are highly prevalent disorders, effect of iron deficiency anaemia on HbA1C need to be evaluated. Many factors such as haemolytic anaemia, vitamin deficiencies, pregnancy. Kidney disease, haemoglobin variants can affect HbA1C levels. This study had two groups of participants. Group 1 included diabetic patients with iron deficiency anaemia and group 2 included diabetic patients without iron deficiency anaemia. Significant correlation between HbA1C and iron deficiency anaemia has been found. PCV, MCH, MCHC, MCV are low in diabetics with Iron deficiency anaemia and high in diabetics without iron deficiency anaemia. Need for anaemia screening before treatment planning of diabetes based on HBA1C levels must be considered and for optimal diabetes control, treating anemia is necessary.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86083596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-15DOI: 10.18231/j.ijcbr.2023.017
Ashwini Pandith
Urinary biochemical analytes are very important tools for clinical decision making. Total allowable error (TEa) by integrating internal (IQC) and external (EQC) quality control performances are used to evaluate the performance of urinary biochemical analytes along with quality specifications strategy. : Alternate 6 months Coefficient of Variation (CV%) and External Quality Assurance Scheme (EQAS) bias% data for urinary biochemistry analytes were collected for the year 2022. TEa calculated for each analyte was calculated based on average CV% and bias%. Total TEa calculated values are compared with optimal, minimal and desirable TEa of each analyte. : TEa values of urinary biochemistry analytes were performing good and fulfilled minimal, desirable and optimal quality requirements except urine creatinine which did not fulfill the minimal standards.: TEa is an excellent quality management tool and quantitatively evaluates analytical performance. The accurate results generated are useful for clinicians for decision-making.
{"title":"Evaluation of analytical performance & quality specification of urine biochemical analytes","authors":"Ashwini Pandith","doi":"10.18231/j.ijcbr.2023.017","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.017","url":null,"abstract":"Urinary biochemical analytes are very important tools for clinical decision making. Total allowable error (TEa) by integrating internal (IQC) and external (EQC) quality control performances are used to evaluate the performance of urinary biochemical analytes along with quality specifications strategy. : Alternate 6 months Coefficient of Variation (CV%) and External Quality Assurance Scheme (EQAS) bias% data for urinary biochemistry analytes were collected for the year 2022. TEa calculated for each analyte was calculated based on average CV% and bias%. Total TEa calculated values are compared with optimal, minimal and desirable TEa of each analyte. : TEa values of urinary biochemistry analytes were performing good and fulfilled minimal, desirable and optimal quality requirements except urine creatinine which did not fulfill the minimal standards.: TEa is an excellent quality management tool and quantitatively evaluates analytical performance. The accurate results generated are useful for clinicians for decision-making.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79602386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Medicinal plants have been used for thousands of years by people around the globe. From ancient times to the end of the nineteenth century, herbs have proven to be the major source of remedial therapies and treatments. Evidence has been collected for ages to reveal the good perspective of herbal plants utilized in numerous supportive, traditional, and different systems, with the ever-enlarging scrutiny of the present-day population with regard to legitimate results. is a predominant medicinal herb that is widely utilized for the cure of many ailments. The sample of was selected from the Uttarkashi Garhwal region of Uttarakhand, and then further identification was done by a designated authority from Dehradun, i.e., the Forest Research Institute (FRI), and steam distillation of fresh leaves was performed and oil extract was collected. The yield of essential oil from leaves was observed and a total of fifty constituents were recognized with the help of Gas Chromatography-Mass Spectrometry (GC-MS). The crucial oil also has antifungal and antibacterial properties against some infectious strains. The engrossing characteristics of the oil constitution were the existence of dictamnol in good amounts. In the current research assignment, plant samples were gathered from the hilly regions of Uttarkashi for oil extraction and identification of chemical constituents.
{"title":"A research on the chemical constituent, characterization and remedial benefits of a native medicinal herb","authors":"Srashti Choudhary, Diksha Panwar, Aditi Tyagi, Kaveri Bisht, Neetu Sharma, B. Bisht, Promila Sharma","doi":"10.18231/j.ijcbr.2023.026","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.026","url":null,"abstract":"Medicinal plants have been used for thousands of years by people around the globe. From ancient times to the end of the nineteenth century, herbs have proven to be the major source of remedial therapies and treatments. Evidence has been collected for ages to reveal the good perspective of herbal plants utilized in numerous supportive, traditional, and different systems, with the ever-enlarging scrutiny of the present-day population with regard to legitimate results. is a predominant medicinal herb that is widely utilized for the cure of many ailments. The sample of was selected from the Uttarkashi Garhwal region of Uttarakhand, and then further identification was done by a designated authority from Dehradun, i.e., the Forest Research Institute (FRI), and steam distillation of fresh leaves was performed and oil extract was collected. The yield of essential oil from leaves was observed and a total of fifty constituents were recognized with the help of Gas Chromatography-Mass Spectrometry (GC-MS). The crucial oil also has antifungal and antibacterial properties against some infectious strains. The engrossing characteristics of the oil constitution were the existence of dictamnol in good amounts. In the current research assignment, plant samples were gathered from the hilly regions of Uttarkashi for oil extraction and identification of chemical constituents.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89732625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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