Pub Date : 2024-02-15DOI: 10.18231/j.ijcbr.2023.058
Dr. Abhinav Manish, Amit Badola
: Gallbladder cancer is a rare malignancy but represents almost 50% of all biliary tract cancer. Biliary cancers are highly fatal malignancies with a 5-year survival rate of approximately 20%. The prognosis of gallbladder cancer is poor due to the aggressive tumor biology, late presentation, complicated anatomic position, and advanced stage at diagnosis. Locally advanced and metastatic disease treatment is with palliative chemotherapy. Alarming sign of gallbladder cancer is overall decreased in incidence in older patients but increased in the younger population. So many mutations have been reported for the gall bladder cancer till date. : A prospective observational study was conducted over a period of 1 year at Asian Institute of Medical Sciences Faridabad which includes hepatobiliary carcinoma patients who are at stage III and stage IV of cancer. After getting the consent formalin fixed paraffin embedded biopsy samples, and 5 ml serum sample was collected in serum separator tube (SST). A whole genome sequencing was performed using Illumina HiSEQ, Illumina (NGS) technology, allows for high-throughput sequencing of DNA and RNA. Illumina's NGS is based on "sequencing by Synthesis" to detect the mutations.: Most common mutation found was in the P53 gene. TP53 (p.Arg175His), TP53 (p.Arg306Ter), TP53 (p.Cys238Tyr), TP53 (p.Leu43Ter), TP53 (p.Glu339Ter), TP53 (p.Pro190Leu). Mutations in the TP53 gene are a common feature of carcinoma of the gallbladder, and are associated with a more aggressive tumor phenotype, resistance to chemotherapy, and poorer overall survival.
:胆囊癌是一种罕见的恶性肿瘤，但几乎占所有胆道癌症的 50%。胆囊癌是高度致命的恶性肿瘤，5 年生存率约为 20%。由于胆囊癌具有侵袭性肿瘤生物学特性、发病较晚、解剖位置复杂以及诊断时已是晚期，因此预后较差。 局部晚期和转移性疾病的治疗以姑息化疗为主。令人担忧的是，胆囊癌在老年患者中的发病率总体下降，但在年轻人群中却有所上升。迄今为止，已有许多关于胆囊癌突变的报道。 : 在法里达巴德亚洲医学科学研究所进行了一项为期一年的前瞻性观察研究，研究对象包括处于癌症 III 期和 IV 期的肝胆癌患者。在征得同意后，在血清分离管（SST）中收集正式固定石蜡包埋活检样本和 5 毫升血清样本。全基因组测序使用的是 Illumina HiSEQ，Illumina（NGS）技术可对 DNA 和 RNA 进行高通量测序。Illumina 的 NGS 基于 "合成测序 "来检测突变：最常见的突变发生在 P53 基因中。TP53（p.Arg175His）、TP53（p.Arg306Ter）、TP53（p.Cys238Tyr）、TP53（p.Leu43Ter）、TP53（p.Glu339Ter）、TP53（p.Pro190Leu）。TP53 基因突变是胆囊癌的常见特征，与更具侵袭性的肿瘤表型、对化疗的耐药性和较差的总生存率有关。
{"title":"Emerging need of molecular profiling in hepatobiliary cancer","authors":"Dr. Abhinav Manish, Amit Badola","doi":"10.18231/j.ijcbr.2023.058","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.058","url":null,"abstract":": Gallbladder cancer is a rare malignancy but represents almost 50% of all biliary tract cancer. Biliary cancers are highly fatal malignancies with a 5-year survival rate of approximately 20%. The prognosis of gallbladder cancer is poor due to the aggressive tumor biology, late presentation, complicated anatomic position, and advanced stage at diagnosis. Locally advanced and metastatic disease treatment is with palliative chemotherapy. Alarming sign of gallbladder cancer is overall decreased in incidence in older patients but increased in the younger population. So many mutations have been reported for the gall bladder cancer till date. : A prospective observational study was conducted over a period of 1 year at Asian Institute of Medical Sciences Faridabad which includes hepatobiliary carcinoma patients who are at stage III and stage IV of cancer. After getting the consent formalin fixed paraffin embedded biopsy samples, and 5 ml serum sample was collected in serum separator tube (SST). A whole genome sequencing was performed using Illumina HiSEQ, Illumina (NGS) technology, allows for high-throughput sequencing of DNA and RNA. Illumina's NGS is based on \"sequencing by Synthesis\" to detect the mutations.: Most common mutation found was in the P53 gene. TP53 (p.Arg175His), TP53 (p.Arg306Ter), TP53 (p.Cys238Tyr), TP53 (p.Leu43Ter), TP53 (p.Glu339Ter), TP53 (p.Pro190Leu). Mutations in the TP53 gene are a common feature of carcinoma of the gallbladder, and are associated with a more aggressive tumor phenotype, resistance to chemotherapy, and poorer overall survival.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"4 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139774474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
COVID-19 was one of the most contagious diseases spread throughout the world and caused by the Corona virus. Its cellular and molecular profiling was performed on individuals suffering from Covid-19-like sickness in Dehradun. During this investigation, 100 random samples were taken from patients. Real Time PCR amplification of suspected cases of Covid-19 was done where 68% of the cases were positive. The proportions and patterns of positive cases and hospital admissions for covid-19 in the third wave in Dehradun were significantly different when compared to the first and second waves. This disparity was caused by a number of factors, including changes in lifestyle, natural immunological ability to cope with recent waves of natural infections, and the most important factor, which was that more than 83% of people over the age of 18 years had received the first or both doses of the vaccine. The Covid-19 vaccines were so effective due to which there was a very low rate of serious infection, hospitalization, and mortality.
{"title":"The Vax effect: Role of global vaccination initiatives in pandemic control and impact in Dehradun during COVID-19","authors":"Ankita Singh, Nivedita Chetan Gurada, Nidhi Mahendru, Abhijeet Kashyap, Sourabh Bhanu, Narotam Sharma, Ritu Kaushik","doi":"10.18231/j.ijcbr.2023.057","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.057","url":null,"abstract":"COVID-19 was one of the most contagious diseases spread throughout the world and caused by the Corona virus. Its cellular and molecular profiling was performed on individuals suffering from Covid-19-like sickness in Dehradun. During this investigation, 100 random samples were taken from patients. Real Time PCR amplification of suspected cases of Covid-19 was done where 68% of the cases were positive. The proportions and patterns of positive cases and hospital admissions for covid-19 in the third wave in Dehradun were significantly different when compared to the first and second waves. This disparity was caused by a number of factors, including changes in lifestyle, natural immunological ability to cope with recent waves of natural infections, and the most important factor, which was that more than 83% of people over the age of 18 years had received the first or both doses of the vaccine. The Covid-19 vaccines were so effective due to which there was a very low rate of serious infection, hospitalization, and mortality.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"12 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139774140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-15DOI: 10.18231/j.ijcbr.2023.060
Poonam Agrawal, Mohit Agrawal, Deepti Chopra
Gout is a metabolic disease characterised by acute or chronic arthritis which is due to deposition of Monosodium urate (MSU) crystals in joints and soft tissues. Classically described as a disease associated with hyperuricemia which may be due to metabolic defect in uric acid production or due to impaired renal excretion.We present a case of 45 year old nondiabetic, non-alcoholic, non-smoker male presented to orthopaedic OPD with complain of pain in right hand and left leg with restriction of joint mobility for past 14 hours.On carefully eliciting the dietary history patient revealed that he was a vegetarian and was on high purine rich diet for past 2 weeks due to family functions and various gatherings. Patient gave the history of consumption of cauliflower, mushrooms, beans along with sweetened carbonated drinks on two consecutive days before the onset of these symptoms.Serum uric acid 4.5 mg/dl, C-reactive protein was 9 mg/dl. To best of our knowledge this is the first case being reported in literatures where purine rich vegetarian diet has induced new case of gout.This kind of gout precipitation after consuming vegetarian diet is rarely reported in literature. This case report emphasizes upon the need of special caution to be taken by persons who are genetically predisposed to gout, while consuming purine rich diet like red meat, seafood, cauliflowers, spinach, rajma, Bengal gram, mushrooms, soft drinks sweetened with fructose.
{"title":"Vegetarian diet induced new onset acute gouty arthritis in a middle-aged genetically predisposed male: A rare presentation","authors":"Poonam Agrawal, Mohit Agrawal, Deepti Chopra","doi":"10.18231/j.ijcbr.2023.060","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.060","url":null,"abstract":"Gout is a metabolic disease characterised by acute or chronic arthritis which is due to deposition of Monosodium urate (MSU) crystals in joints and soft tissues. Classically described as a disease associated with hyperuricemia which may be due to metabolic defect in uric acid production or due to impaired renal excretion.We present a case of 45 year old nondiabetic, non-alcoholic, non-smoker male presented to orthopaedic OPD with complain of pain in right hand and left leg with restriction of joint mobility for past 14 hours.On carefully eliciting the dietary history patient revealed that he was a vegetarian and was on high purine rich diet for past 2 weeks due to family functions and various gatherings. Patient gave the history of consumption of cauliflower, mushrooms, beans along with sweetened carbonated drinks on two consecutive days before the onset of these symptoms.Serum uric acid 4.5 mg/dl, C-reactive protein was 9 mg/dl. To best of our knowledge this is the first case being reported in literatures where purine rich vegetarian diet has induced new case of gout.This kind of gout precipitation after consuming vegetarian diet is rarely reported in literature. This case report emphasizes upon the need of special caution to be taken by persons who are genetically predisposed to gout, while consuming purine rich diet like red meat, seafood, cauliflowers, spinach, rajma, Bengal gram, mushrooms, soft drinks sweetened with fructose.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"109 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139834822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-15DOI: 10.18231/j.ijcbr.2023.049
Kowsalya R
Among men one of the most common cancers identified globally is prostate cancer. Although the serum prostate specific antigen remains important for prognosis and diagnosis, the PSA assay is not highly accurate. In the search for improved minimally invasive methods as a biomarker, the expression patterns of circulating miRNAs have a potential importance and are emerging as a promising candidate as prognostic markers for prostate cancer.
{"title":"MicroRNAs as biomarkers in prostate cancer: A mini review","authors":"Kowsalya R","doi":"10.18231/j.ijcbr.2023.049","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.049","url":null,"abstract":"Among men one of the most common cancers identified globally is prostate cancer. Although the serum prostate specific antigen remains important for prognosis and diagnosis, the PSA assay is not highly accurate. In the search for improved minimally invasive methods as a biomarker, the expression patterns of circulating miRNAs have a potential importance and are emerging as a promising candidate as prognostic markers for prostate cancer.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"320 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139834731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-15DOI: 10.18231/j.ijcbr.2023.053
S. Shrestha, Pradeep Krishna Shrestha, Binod Kumar Yadav
Nepalese owing to modern lifestyle and processed food are racially at an elevated risk of acquiring central obesity-related insulin resistance (IR) and thus Type 2 diabetes mellitus (T2DM) and Diabetic Kidney Disease (DKD). Low birth weight in utero and later becoming obese risks the development of T2DM. In a total of eighty-four newly diagnosed treatment-naive Nepalese T2DMs, incidence of IR, percentage beta-cell function (%BCF) and percentage insulin sensitivity (%IS) were determined using Homeostatic Model Assessment 2 (HOMA2-IR). Association of HOMA2-IR with albuminuria, kidney function, hs-CRP, fatty liver, fatty pancreas, several anthropometric and biochemical parameters were analyzed. Among the eighty-four T2DMs, fifty-four agreeing regular follow-ups were prescribed a low-carbohydrate diet (<130gm/day). At 6 months, their glycemic controls were monitored. From 84 newly diagnosed T2DMs, 56 (66.7%) were insulin resistant and 28(33.3%) insulin-sensitive on HOMA2-IR. There was a significant association of HOMA2-IR with albuminuria and declining kidney function (p=0.006 and 0.034 respectively) and most of them were at reversible stages. Waist circumference (WC), waist-hip ratio (WHR), lipid profile ratios, fatty liver and fatty pancreas were elucidated as potential markers for IR. The IS group (ISG) had significantly inadequate %BCF (p=0.001) but high %IS (p<0.001) has healthier WHR (p=0.001) and lipid profile ratios which are opposite to IR group (IRG). 13 ISG had raised hs-CRP and 15 normal and 21 IRG had normal hs-CRP and 35 raised. At 6 months, the IRG achieved significantly better postprandial glycemic goals (p=0.04) and significant improvement in WC and WHR (p=0.008 and 0.03 respectively) with a low-carbohydrate diet as compared to ISG. Severe insulin resistance and IR-associated DKD, fatty liver and fatty pancreas are highly prevalent from the time of diagnosis of T2DM in the Nepalese population. Thus inspecting for IR and its consequences mandatorily at diagnosis and applying precision therapies like adjustments in the quality and quantity of staple food carbohydrates significantly improves IR-related parameters and glycemia.
{"title":"A mandatory practice in type 2 diabetes mellitus to maintain quality of life","authors":"S. Shrestha, Pradeep Krishna Shrestha, Binod Kumar Yadav","doi":"10.18231/j.ijcbr.2023.053","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.053","url":null,"abstract":"Nepalese owing to modern lifestyle and processed food are racially at an elevated risk of acquiring central obesity-related insulin resistance (IR) and thus Type 2 diabetes mellitus (T2DM) and Diabetic Kidney Disease (DKD). Low birth weight in utero and later becoming obese risks the development of T2DM. In a total of eighty-four newly diagnosed treatment-naive Nepalese T2DMs, incidence of IR, percentage beta-cell function (%BCF) and percentage insulin sensitivity (%IS) were determined using Homeostatic Model Assessment 2 (HOMA2-IR). Association of HOMA2-IR with albuminuria, kidney function, hs-CRP, fatty liver, fatty pancreas, several anthropometric and biochemical parameters were analyzed. Among the eighty-four T2DMs, fifty-four agreeing regular follow-ups were prescribed a low-carbohydrate diet (<130gm/day). At 6 months, their glycemic controls were monitored. From 84 newly diagnosed T2DMs, 56 (66.7%) were insulin resistant and 28(33.3%) insulin-sensitive on HOMA2-IR. There was a significant association of HOMA2-IR with albuminuria and declining kidney function (p=0.006 and 0.034 respectively) and most of them were at reversible stages. Waist circumference (WC), waist-hip ratio (WHR), lipid profile ratios, fatty liver and fatty pancreas were elucidated as potential markers for IR. The IS group (ISG) had significantly inadequate %BCF (p=0.001) but high %IS (p<0.001) has healthier WHR (p=0.001) and lipid profile ratios which are opposite to IR group (IRG). 13 ISG had raised hs-CRP and 15 normal and 21 IRG had normal hs-CRP and 35 raised. At 6 months, the IRG achieved significantly better postprandial glycemic goals (p=0.04) and significant improvement in WC and WHR (p=0.008 and 0.03 respectively) with a low-carbohydrate diet as compared to ISG. Severe insulin resistance and IR-associated DKD, fatty liver and fatty pancreas are highly prevalent from the time of diagnosis of T2DM in the Nepalese population. Thus inspecting for IR and its consequences mandatorily at diagnosis and applying precision therapies like adjustments in the quality and quantity of staple food carbohydrates significantly improves IR-related parameters and glycemia.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"508 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139834872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-15DOI: 10.18231/j.ijcbr.2023.048
Abdullah Salim Al-Karawi, A. Kadhim, Maha M Kadum
It is an in-depth analysis of a globally significant infectious disease, tuberculosis (TB). It covers the epidemiology, pathogenesis, diagnosis, treatment and prevention of TB with attention to its successes and shortcomings. The review examines this complicated relationship between Mycobacterium tuberculosis and the human host. It describes how outcomes move from latent infection to active disease. An Overview It introduces the current diagnostic methods, therapeutic regimens and preventive strategies; repeated emphasis is placed on targeted interventions and public health efforts. Also, the review covers future efforts in TB research and control that will further improve prevention and treatment. These include vaccines; new drugs (including a quest for novel agents); improved diagnostics (such as more rapid tests or smear-free methods); public health interventions such as policies on occupational exposures to respiratory rusts; integrated care models including continuity of services between Through these points the review illuminates how much can still be accomplished in terms of controlling--and even eliminating entirely--TB, with ramifications for global public health.
{"title":"Recent advances in tuberculosis: A comprehensive review of emerging trends in pathogenesis, diagnostics, treatment, and prevention","authors":"Abdullah Salim Al-Karawi, A. Kadhim, Maha M Kadum","doi":"10.18231/j.ijcbr.2023.048","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.048","url":null,"abstract":"It is an in-depth analysis of a globally significant infectious disease, tuberculosis (TB). It covers the epidemiology, pathogenesis, diagnosis, treatment and prevention of TB with attention to its successes and shortcomings. The review examines this complicated relationship between Mycobacterium tuberculosis and the human host. It describes how outcomes move from latent infection to active disease. An Overview It introduces the current diagnostic methods, therapeutic regimens and preventive strategies; repeated emphasis is placed on targeted interventions and public health efforts. Also, the review covers future efforts in TB research and control that will further improve prevention and treatment. These include vaccines; new drugs (including a quest for novel agents); improved diagnostics (such as more rapid tests or smear-free methods); public health interventions such as policies on occupational exposures to respiratory rusts; integrated care models including continuity of services between Through these points the review illuminates how much can still be accomplished in terms of controlling--and even eliminating entirely--TB, with ramifications for global public health.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"510 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139834109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-15DOI: 10.18231/j.ijcbr.2023.052
Yao Epse Yapo Carine Mireille, Y. Eric, Kouacou Kadio Morel, Konan Konan Jean-Louis, Kone Dakouri Benedicte
Parathyroid hormone (PTH) testing is recommended once or twice a year to prevent bone complications resulting from chronic kidney disease. However, its interpretation is subject to many factors, including the generation of the PTH test used. For this reason, it is recommended that each laboratory establish its own PTH Upper Limit of Normality (ULN). In order to guarantee optimal management of hemodialysis patients in the public sector in Côte d'Ivoire (West Africa), it proved important to compare the diagnosis of hyperparathyroidism made using two platforms used in the laboratory in charge of monitoring these patients: PTH VIDAS® (3rd Generation) and PTH MAGLUMI® (2nd Generation). This was a cross-sectional study of 65 haemodialysis patients in Abidjan. PTH determinations were performed simultaneously on VIDAS® and MAGLUMI® platforms. The results obtained were interpreted on the basis of PTH threshold values pre-established in the laboratory. 86.15% of patients had concordant diagnoses on both platforms, compared with 13.85% with discordant diagnoses. Statistical analysis of these results showed a non-statistically significant difference.: The limitation posed by differences in PTH concentration given by different platforms can be circumvented by using PTH ULN stablished for each platform for a given population. Conclusion: The diagnoses made by the two platforms are thus superimposable using their respective PTH ULN.
{"title":"Comparison of two parathyroid hormone (pth) assay methods in the monitoring of black african hemodialysis patients in the ivory coast (Maglumi® vs Vidas Biomerieux® Kits)","authors":"Yao Epse Yapo Carine Mireille, Y. Eric, Kouacou Kadio Morel, Konan Konan Jean-Louis, Kone Dakouri Benedicte","doi":"10.18231/j.ijcbr.2023.052","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.052","url":null,"abstract":"Parathyroid hormone (PTH) testing is recommended once or twice a year to prevent bone complications resulting from chronic kidney disease. However, its interpretation is subject to many factors, including the generation of the PTH test used. For this reason, it is recommended that each laboratory establish its own PTH Upper Limit of Normality (ULN). In order to guarantee optimal management of hemodialysis patients in the public sector in Côte d'Ivoire (West Africa), it proved important to compare the diagnosis of hyperparathyroidism made using two platforms used in the laboratory in charge of monitoring these patients: PTH VIDAS® (3rd Generation) and PTH MAGLUMI® (2nd Generation). This was a cross-sectional study of 65 haemodialysis patients in Abidjan. PTH determinations were performed simultaneously on VIDAS® and MAGLUMI® platforms. The results obtained were interpreted on the basis of PTH threshold values pre-established in the laboratory. 86.15% of patients had concordant diagnoses on both platforms, compared with 13.85% with discordant diagnoses. Statistical analysis of these results showed a non-statistically significant difference.: The limitation posed by differences in PTH concentration given by different platforms can be circumvented by using PTH ULN stablished for each platform for a given population. Conclusion: The diagnoses made by the two platforms are thus superimposable using their respective PTH ULN.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"136 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139834635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-15DOI: 10.18231/j.ijcbr.2023.047
Uma Maheshwari K
{"title":"Artificial intelligence in clinical chemistry – Boon or a bane","authors":"Uma Maheshwari K","doi":"10.18231/j.ijcbr.2023.047","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.047","url":null,"abstract":"","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"24 14","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139776209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.18231/j.ijcbr.2023.039
Nevin K Govindan, Jayakumar K Kochukunju
The common filarial worm of cattle, , whose larvae are known as , are discovered in the blood and peritoneum and cause a condition known as cerebrospinal nematodiasis. There is an unavoidable need for prompt detection and treatment of these filarial diseases. Infections caused by a variety of filarial species have traditionally been treated with diethylcarbamazine (DEC). The most notable aspect of DEC is that, , it appears to have little effect on filarial parasites. However, DEC may act on the parasite's surface, unmasking antigen. This study was initiated to evaluate the effect of DEC on the antioxidant enzyme activities, Superoxide dismutase (SOD), and Catalase (CAT) of mice infected with (Mf). was collected and the Mf was isolated and injected into Swiss-bred mice for 12 days. The animals were grouped as Control, Control +DEC, Control + Mf, and Control+Mf+DEC. The results showed that CAT activity in the peritoneal fluid of Control+Mf+DEC was found to be elevated at a higher level compared to the control. The activity in the Control+DEC and Control+Mf mice showed similar values. SOD activity in the peritoneal fluid of Control+Mf+DEC mice remained elevated from the 1 day to the 12 day compared to the control. Though DEC alone treated mice did not show any profound increase in the activity of these enzymes. The rise in SOD level during the 3 and 5 day of the DEC-treated infected mice may be due to the production of active oxygen species. Proposed to occur as a result of a tilt in the biochemical balance of the cells towards the oxidative damage, failing which decreased removal of free radicals leading to tissue damage results. The result showed that the DEC treatment may be affecting the survival of the Mf shown by the increased antioxidant enzymes which may be due to the inflammation caused by the excretory products of Mf.
{"title":"Diethylcarbamazine modulates the antioxidant enzyme activity in proxy host mice infected with the Setaria microfilariae","authors":"Nevin K Govindan, Jayakumar K Kochukunju","doi":"10.18231/j.ijcbr.2023.039","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.039","url":null,"abstract":"The common filarial worm of cattle, , whose larvae are known as , are discovered in the blood and peritoneum and cause a condition known as cerebrospinal nematodiasis. There is an unavoidable need for prompt detection and treatment of these filarial diseases. Infections caused by a variety of filarial species have traditionally been treated with diethylcarbamazine (DEC). The most notable aspect of DEC is that, , it appears to have little effect on filarial parasites. However, DEC may act on the parasite's surface, unmasking antigen. This study was initiated to evaluate the effect of DEC on the antioxidant enzyme activities, Superoxide dismutase (SOD), and Catalase (CAT) of mice infected with (Mf). was collected and the Mf was isolated and injected into Swiss-bred mice for 12 days. The animals were grouped as Control, Control +DEC, Control + Mf, and Control+Mf+DEC. The results showed that CAT activity in the peritoneal fluid of Control+Mf+DEC was found to be elevated at a higher level compared to the control. The activity in the Control+DEC and Control+Mf mice showed similar values. SOD activity in the peritoneal fluid of Control+Mf+DEC mice remained elevated from the 1 day to the 12 day compared to the control. Though DEC alone treated mice did not show any profound increase in the activity of these enzymes. The rise in SOD level during the 3 and 5 day of the DEC-treated infected mice may be due to the production of active oxygen species. Proposed to occur as a result of a tilt in the biochemical balance of the cells towards the oxidative damage, failing which decreased removal of free radicals leading to tissue damage results. The result showed that the DEC treatment may be affecting the survival of the Mf shown by the increased antioxidant enzymes which may be due to the inflammation caused by the excretory products of Mf.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"76 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135760101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.18231/j.ijcbr.2023.044
Carlos Fernando Mourão, Adam Lowenstein, Mona Patel
Leukocyte platelet-rich fibrin (L-PRF) is an autologous biomaterial used in regenerative medicine and tissue healing. This review emphasizes the role of growth factors and cytokines in L-PRF, which contribute significantly to the healing process in various clinical scenarios. The analysis focuses on the controlled release of Platelet-derived growth factors (PDGF), Fibroblast growth factors (FGF), and Vascular endothelial growth factors (VEGF), demonstrating a propensity to promote angiogenesis and stimulate the migration and proliferation of cells necessary for tissue regeneration. The review also examines the roles of anti-inflammatory and pro-inflammatory cytokines, including Interleukins (IL-1, IL-4, IL-10, IL-6) and Tumor necrosis factor-alpha (TNF-α), and Interferon-gamma (IFN-γ), in modulating the inflammatory response during tissue healing. The goal of this comprehensive review is to enhance the understanding of L-PRF and its constituent factors, opening avenues for harnessing its potential in regenerative medicine. The bioactive components within L-PRF stimulate tissue healing and repair, creating an optimal environment for tissue regeneration.
{"title":"Harvesting the biochemical potential of L-PRF with their growth factors and cytokines","authors":"Carlos Fernando Mourão, Adam Lowenstein, Mona Patel","doi":"10.18231/j.ijcbr.2023.044","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.044","url":null,"abstract":"Leukocyte platelet-rich fibrin (L-PRF) is an autologous biomaterial used in regenerative medicine and tissue healing. This review emphasizes the role of growth factors and cytokines in L-PRF, which contribute significantly to the healing process in various clinical scenarios. The analysis focuses on the controlled release of Platelet-derived growth factors (PDGF), Fibroblast growth factors (FGF), and Vascular endothelial growth factors (VEGF), demonstrating a propensity to promote angiogenesis and stimulate the migration and proliferation of cells necessary for tissue regeneration. The review also examines the roles of anti-inflammatory and pro-inflammatory cytokines, including Interleukins (IL-1, IL-4, IL-10, IL-6) and Tumor necrosis factor-alpha (TNF-α), and Interferon-gamma (IFN-γ), in modulating the inflammatory response during tissue healing. The goal of this comprehensive review is to enhance the understanding of L-PRF and its constituent factors, opening avenues for harnessing its potential in regenerative medicine. The bioactive components within L-PRF stimulate tissue healing and repair, creating an optimal environment for tissue regeneration.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"143 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135760108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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