Pub Date : 2024-02-15DOI: 10.18231/j.ijcbr.2023.047
Uma Maheshwari K
{"title":"Artificial intelligence in clinical chemistry – Boon or a bane","authors":"Uma Maheshwari K","doi":"10.18231/j.ijcbr.2023.047","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.047","url":null,"abstract":"","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"301 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139835625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Inborn errors of metabolism are rare inherited disorders which leads to significant morbidity and mortality in patients. Very few studies have been conducted in India to assess prevalence of Inborn Errors of Metabolism (IEM) in newborns. We proposed testing by TMS/TR-FIA followed by NGS. This pilot study would be one of the first expanded NBS studies in India.The aim of this study was to determine the prevalence of IEM in newborns based on the samples received at Metropolis Global Reference Lab, India. Next-generation sequencing (NGS) was done as a confirmational analysis for patients tested presumptive positive on Newborn screening using Tandem Mass spectrometry (TMS) and Time-resolved fluoroimmunoassay (TR-FIA). Two years retrospective study was conducted based on incidences of IEM using TMS and TR-FIA. NGS testing was performed on presumptive positive newborns for cystic fibrosis (CF), galactosemia and urea cycle disorder/ organic academia (UCD /OA) who had undergone NBS by TMS and TR-FIA. Highestprevalence of 1.98% & 1.58% was detected for G6PD and TSH respectively by TR-FIA. Prevalence of AA disorders (3.20%), OA (1.60%) and UCD (1.43%) was observed to be the highest amongst the diseases detected by TMS. Presumptive positive case of Argininemia and Cystic Fibrosis were found to be concordant with NGS. Out of three presumptive positive cases, one presumptive positive case of CF and two of galactose were found discordant. Our prevalence study showed similarities to the prevalence reports published by other Asian countries. Expanded NBS program can be improved by including NGS as a first follow-up test after detection of abnormal metabolites in DBS. This approach will help in reducing the encumbrance of false-positive as well as false-negative cases. Our study will be influential in conducting more prospective studies and routine implementation of NGS-based analysis in NBS in India.
{"title":"Expanding the screening of newborns for detecting inborn errors in metabolism using next generation sequencing following mass spectrometry/immunoassay","authors":"A. Christy, Eatu Das, Jasmin Surana, Pradnya Padhye, Kedar Shirodkar, Rakhi Bajpai Dixit, Kirti Chadha","doi":"10.18231/j.ijcbr.2023.059","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.059","url":null,"abstract":"Inborn errors of metabolism are rare inherited disorders which leads to significant morbidity and mortality in patients. Very few studies have been conducted in India to assess prevalence of Inborn Errors of Metabolism (IEM) in newborns. We proposed testing by TMS/TR-FIA followed by NGS. This pilot study would be one of the first expanded NBS studies in India.The aim of this study was to determine the prevalence of IEM in newborns based on the samples received at Metropolis Global Reference Lab, India. Next-generation sequencing (NGS) was done as a confirmational analysis for patients tested presumptive positive on Newborn screening using Tandem Mass spectrometry (TMS) and Time-resolved fluoroimmunoassay (TR-FIA). Two years retrospective study was conducted based on incidences of IEM using TMS and TR-FIA. NGS testing was performed on presumptive positive newborns for cystic fibrosis (CF), galactosemia and urea cycle disorder/ organic academia (UCD /OA) who had undergone NBS by TMS and TR-FIA. Highestprevalence of 1.98% & 1.58% was detected for G6PD and TSH respectively by TR-FIA. Prevalence of AA disorders (3.20%), OA (1.60%) and UCD (1.43%) was observed to be the highest amongst the diseases detected by TMS. Presumptive positive case of Argininemia and Cystic Fibrosis were found to be concordant with NGS. Out of three presumptive positive cases, one presumptive positive case of CF and two of galactose were found discordant. Our prevalence study showed similarities to the prevalence reports published by other Asian countries. Expanded NBS program can be improved by including NGS as a first follow-up test after detection of abnormal metabolites in DBS. This approach will help in reducing the encumbrance of false-positive as well as false-negative cases. Our study will be influential in conducting more prospective studies and routine implementation of NGS-based analysis in NBS in India.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"43 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139775676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-15DOI: 10.18231/j.ijcbr.2023.050
D. Trivedi, Priyali Purandare
Sickle cell anaemia is a molecular disease. WHO recognises it as a global public health problem. In India, it is common among tribal communities. Increased HbS is a culprit. Therapeutic research is focused on maintaining high levels of HbF and decreasing 2,3, BPG to target disease. To assess the role of naturally compensated haemoglobin variants in tribal Sickle cell anaemic patients of North East Gujarat. Prospective, analytical, case control study conducted on randomly selected fifty tribal Sickle cell anaemic patients having disease for more than 5 years. Fifty age and sex matched, healthy control subjects. Each fifty Tribal sickle cell anaemic patients and healthy control were included in the study. Total Haemoglobin level, Sickling test by NESTROFT method and Haemoglobin variants were analysed by alkaline haemoglobin electrophoresis. Frequencies of clinical crises were recorded by oral questioning. The results were analysed using SPSS version 20. Student unpaired t- test was employed to assess the significance of the differences. P-values < 0.05 considered statistically significant. We observed decreased levels of total haemoglobin, high levels of HbF and HbA2 along with reduced HbA0. Compensatory increase of HbF in tribal SCA patients have shown lower frequency of clinical crises. Reduced HbA0 in sickle cell anaemia is compensated by naturally elevated HbF and HbA2 in tribal patients which has a beneficial influence on their general health.
{"title":"Elevated foetal haemoglobin in tribal sickle cell anaemic patients: Blessing in disgust?","authors":"D. Trivedi, Priyali Purandare","doi":"10.18231/j.ijcbr.2023.050","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.050","url":null,"abstract":"Sickle cell anaemia is a molecular disease. WHO recognises it as a global public health problem. In India, it is common among tribal communities. Increased HbS is a culprit. Therapeutic research is focused on maintaining high levels of HbF and decreasing 2,3, BPG to target disease. To assess the role of naturally compensated haemoglobin variants in tribal Sickle cell anaemic patients of North East Gujarat. Prospective, analytical, case control study conducted on randomly selected fifty tribal Sickle cell anaemic patients having disease for more than 5 years. Fifty age and sex matched, healthy control subjects. Each fifty Tribal sickle cell anaemic patients and healthy control were included in the study. Total Haemoglobin level, Sickling test by NESTROFT method and Haemoglobin variants were analysed by alkaline haemoglobin electrophoresis. Frequencies of clinical crises were recorded by oral questioning. The results were analysed using SPSS version 20. Student unpaired t- test was employed to assess the significance of the differences. P-values < 0.05 considered statistically significant. We observed decreased levels of total haemoglobin, high levels of HbF and HbA2 along with reduced HbA0. Compensatory increase of HbF in tribal SCA patients have shown lower frequency of clinical crises. Reduced HbA0 in sickle cell anaemia is compensated by naturally elevated HbF and HbA2 in tribal patients which has a beneficial influence on their general health.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"208 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139833673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-15DOI: 10.18231/j.ijcbr.2023.058
Dr. Abhinav Manish, Amit Badola
: Gallbladder cancer is a rare malignancy but represents almost 50% of all biliary tract cancer. Biliary cancers are highly fatal malignancies with a 5-year survival rate of approximately 20%. The prognosis of gallbladder cancer is poor due to the aggressive tumor biology, late presentation, complicated anatomic position, and advanced stage at diagnosis. Locally advanced and metastatic disease treatment is with palliative chemotherapy. Alarming sign of gallbladder cancer is overall decreased in incidence in older patients but increased in the younger population. So many mutations have been reported for the gall bladder cancer till date. : A prospective observational study was conducted over a period of 1 year at Asian Institute of Medical Sciences Faridabad which includes hepatobiliary carcinoma patients who are at stage III and stage IV of cancer. After getting the consent formalin fixed paraffin embedded biopsy samples, and 5 ml serum sample was collected in serum separator tube (SST). A whole genome sequencing was performed using Illumina HiSEQ, Illumina (NGS) technology, allows for high-throughput sequencing of DNA and RNA. Illumina's NGS is based on "sequencing by Synthesis" to detect the mutations.: Most common mutation found was in the P53 gene. TP53 (p.Arg175His), TP53 (p.Arg306Ter), TP53 (p.Cys238Tyr), TP53 (p.Leu43Ter), TP53 (p.Glu339Ter), TP53 (p.Pro190Leu). Mutations in the TP53 gene are a common feature of carcinoma of the gallbladder, and are associated with a more aggressive tumor phenotype, resistance to chemotherapy, and poorer overall survival.
:胆囊癌是一种罕见的恶性肿瘤，但几乎占所有胆道癌症的 50%。胆囊癌是高度致命的恶性肿瘤，5 年生存率约为 20%。由于胆囊癌具有侵袭性肿瘤生物学特性、发病较晚、解剖位置复杂以及诊断时已是晚期，因此预后较差。 局部晚期和转移性疾病的治疗以姑息化疗为主。令人担忧的是，胆囊癌在老年患者中的发病率总体下降，但在年轻人群中却有所上升。迄今为止，已有许多关于胆囊癌突变的报道。 : 在法里达巴德亚洲医学科学研究所进行了一项为期一年的前瞻性观察研究，研究对象包括处于癌症 III 期和 IV 期的肝胆癌患者。在征得同意后，在血清分离管（SST）中收集正式固定石蜡包埋活检样本和 5 毫升血清样本。全基因组测序使用的是 Illumina HiSEQ，Illumina（NGS）技术可对 DNA 和 RNA 进行高通量测序。Illumina 的 NGS 基于 "合成测序 "来检测突变：最常见的突变发生在 P53 基因中。TP53（p.Arg175His）、TP53（p.Arg306Ter）、TP53（p.Cys238Tyr）、TP53（p.Leu43Ter）、TP53（p.Glu339Ter）、TP53（p.Pro190Leu）。TP53 基因突变是胆囊癌的常见特征，与更具侵袭性的肿瘤表型、对化疗的耐药性和较差的总生存率有关。
{"title":"Emerging need of molecular profiling in hepatobiliary cancer","authors":"Dr. Abhinav Manish, Amit Badola","doi":"10.18231/j.ijcbr.2023.058","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.058","url":null,"abstract":": Gallbladder cancer is a rare malignancy but represents almost 50% of all biliary tract cancer. Biliary cancers are highly fatal malignancies with a 5-year survival rate of approximately 20%. The prognosis of gallbladder cancer is poor due to the aggressive tumor biology, late presentation, complicated anatomic position, and advanced stage at diagnosis. Locally advanced and metastatic disease treatment is with palliative chemotherapy. Alarming sign of gallbladder cancer is overall decreased in incidence in older patients but increased in the younger population. So many mutations have been reported for the gall bladder cancer till date. : A prospective observational study was conducted over a period of 1 year at Asian Institute of Medical Sciences Faridabad which includes hepatobiliary carcinoma patients who are at stage III and stage IV of cancer. After getting the consent formalin fixed paraffin embedded biopsy samples, and 5 ml serum sample was collected in serum separator tube (SST). A whole genome sequencing was performed using Illumina HiSEQ, Illumina (NGS) technology, allows for high-throughput sequencing of DNA and RNA. Illumina's NGS is based on \"sequencing by Synthesis\" to detect the mutations.: Most common mutation found was in the P53 gene. TP53 (p.Arg175His), TP53 (p.Arg306Ter), TP53 (p.Cys238Tyr), TP53 (p.Leu43Ter), TP53 (p.Glu339Ter), TP53 (p.Pro190Leu). Mutations in the TP53 gene are a common feature of carcinoma of the gallbladder, and are associated with a more aggressive tumor phenotype, resistance to chemotherapy, and poorer overall survival.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"417 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139834268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-15DOI: 10.18231/j.ijcbr.2023.048
Abdullah Salim Al-Karawi, A. Kadhim, Maha M Kadum
It is an in-depth analysis of a globally significant infectious disease, tuberculosis (TB). It covers the epidemiology, pathogenesis, diagnosis, treatment and prevention of TB with attention to its successes and shortcomings. The review examines this complicated relationship between Mycobacterium tuberculosis and the human host. It describes how outcomes move from latent infection to active disease. An Overview It introduces the current diagnostic methods, therapeutic regimens and preventive strategies; repeated emphasis is placed on targeted interventions and public health efforts. Also, the review covers future efforts in TB research and control that will further improve prevention and treatment. These include vaccines; new drugs (including a quest for novel agents); improved diagnostics (such as more rapid tests or smear-free methods); public health interventions such as policies on occupational exposures to respiratory rusts; integrated care models including continuity of services between Through these points the review illuminates how much can still be accomplished in terms of controlling--and even eliminating entirely--TB, with ramifications for global public health.
{"title":"Recent advances in tuberculosis: A comprehensive review of emerging trends in pathogenesis, diagnostics, treatment, and prevention","authors":"Abdullah Salim Al-Karawi, A. Kadhim, Maha M Kadum","doi":"10.18231/j.ijcbr.2023.048","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.048","url":null,"abstract":"It is an in-depth analysis of a globally significant infectious disease, tuberculosis (TB). It covers the epidemiology, pathogenesis, diagnosis, treatment and prevention of TB with attention to its successes and shortcomings. The review examines this complicated relationship between Mycobacterium tuberculosis and the human host. It describes how outcomes move from latent infection to active disease. An Overview It introduces the current diagnostic methods, therapeutic regimens and preventive strategies; repeated emphasis is placed on targeted interventions and public health efforts. Also, the review covers future efforts in TB research and control that will further improve prevention and treatment. These include vaccines; new drugs (including a quest for novel agents); improved diagnostics (such as more rapid tests or smear-free methods); public health interventions such as policies on occupational exposures to respiratory rusts; integrated care models including continuity of services between Through these points the review illuminates how much can still be accomplished in terms of controlling--and even eliminating entirely--TB, with ramifications for global public health.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"15 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139774382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-15DOI: 10.18231/j.ijcbr.2023.054
Roshna Vijay
: SARS-CoV2 infection induces inflammatory responses and acute lung injury in human beings. Infection causes certain haematological and biochemical changes in patients. This study aimed analyze the correlation between CBC derivatives and biochemical prognostic marker in COVID-19.: Total 80 individuals selected, 50 COVID-19 positive patients and 30 subjects negative for COVID-19 RT-PCR test. Blood collected was sent to NLR, RDW, assayed in Beckman Coulter DxH 800. Serum CRP estimated by Immuno-turbidimetric method, ferritin by CLIA. Plasma collected estimate for D-DIMER by CLIA. Unpaired T test & Pearson correlation (IBM SPSS 22.0) were used. : Elevated levels of NLR (p value <0.001), RDW (p value <0.001), FERRITIN (p value <0.001), D-D-DIMER (p value <0.001), CRP (p value <0.001) some research parameters like LHD and RSF show significant decrease. In current study the NLR positively correlate to CRP (r value=0.5, p value <0.001), NLR positively correlate to D-DIMER (r value=0.3, p value<0.05), RDW positively correlated to CRP (r value=, 0.3, p value <0.05), RDW negatively correlated to ferritin (r value=-0.439, p value <0.001)LHD negatively correlated ferritin (r value=-0.316, p value=<0.05), MAF negatively correlated to D-dimer (r value=-0.244 <0.05), MSCV positively correlated with CRP (r value=0.336, p value= <0.05).: The cytokine storm produced during covid-19 infection which inturn develop neutrophilia and lymphopenia, CRP production and also cause elevation of procoagulants &D-dimer. RDW and CRP are positively correlated they are elevated during inflammatory conditions.RDW and FERRITIN show negative correlation. Retrospective study have some limitation so future studies will reveal more information.
{"title":"Correlation study of CBC derivatives and biochemical prognostic marker in covid-19 patients","authors":"Roshna Vijay","doi":"10.18231/j.ijcbr.2023.054","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.054","url":null,"abstract":": SARS-CoV2 infection induces inflammatory responses and acute lung injury in human beings. Infection causes certain haematological and biochemical changes in patients. This study aimed analyze the correlation between CBC derivatives and biochemical prognostic marker in COVID-19.: Total 80 individuals selected, 50 COVID-19 positive patients and 30 subjects negative for COVID-19 RT-PCR test. Blood collected was sent to NLR, RDW, assayed in Beckman Coulter DxH 800. Serum CRP estimated by Immuno-turbidimetric method, ferritin by CLIA. Plasma collected estimate for D-DIMER by CLIA. Unpaired T test & Pearson correlation (IBM SPSS 22.0) were used. : Elevated levels of NLR (p value <0.001), RDW (p value <0.001), FERRITIN (p value <0.001), D-D-DIMER (p value <0.001), CRP (p value <0.001) some research parameters like LHD and RSF show significant decrease. In current study the NLR positively correlate to CRP (r value=0.5, p value <0.001), NLR positively correlate to D-DIMER (r value=0.3, p value<0.05), RDW positively correlated to CRP (r value=, 0.3, p value <0.05), RDW negatively correlated to ferritin (r value=-0.439, p value <0.001)LHD negatively correlated ferritin (r value=-0.316, p value=<0.05), MAF negatively correlated to D-dimer (r value=-0.244 <0.05), MSCV positively correlated with CRP (r value=0.336, p value= <0.05).: The cytokine storm produced during covid-19 infection which inturn develop neutrophilia and lymphopenia, CRP production and also cause elevation of procoagulants &D-dimer. RDW and CRP are positively correlated they are elevated during inflammatory conditions.RDW and FERRITIN show negative correlation. Retrospective study have some limitation so future studies will reveal more information.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"93 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139774762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-15DOI: 10.18231/j.ijcbr.2023.051
Dere Kwadjo Anicet Luc, K. Gervais, Manhan Kahissie Noel, Fofana Seguenan, Tuo Wako-Tianwa Alice, T. G. Georges
: Diabetes keto-acidosis is the most serious hyperglycemic emergency in patients with diabetes. DKA has long been considered a key clinical feature of T1D. In this study, we assessed biochemical disorders focused on lipid and renal parameters in KPD.: We carried out a case-controls study for one year, encompassing 200 participants in total. Patients were outpatients newly diagnosed diabetics attending for the first time hospitals, controls were no-ketosis participants. Blood samples were taken, after 10 to 12 hours of fasting from the day before, in different tubes for each intended analyses according to instructor’s recommendation. The increased mean value of fasting blood glucose together with HbA1c are corrolated with ketonuria. As regard to cardio-metabolic risk, ketosis-prone patients were at greater risk than no-ketosis controls The mean AIP value was higher in KPD women than in men, with no significant statistical correlation. Uremia and creatininemia, two kidney parameters commonly prescribed to explore glomerular filtration, were correlated with some social indicators. The mean values of these both parameters increased significantly with age in KPD group. Creatinine increased significantly with age in patients, in line with glomerular filtration rate (GFR). The older the participants, the higher the mean values of these parameters. : These biochemical parameters, high atherogenic index and lower glomerular filtration rate, documented in our study, must be sought out when inaugural ketosis is suspected for improving the clinical prognosis of patients in the initial step of management.
{"title":"Biochemical profile of patients in ketosis-prone diabetes state in Côte d’Ivoire","authors":"Dere Kwadjo Anicet Luc, K. Gervais, Manhan Kahissie Noel, Fofana Seguenan, Tuo Wako-Tianwa Alice, T. G. Georges","doi":"10.18231/j.ijcbr.2023.051","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.051","url":null,"abstract":": Diabetes keto-acidosis is the most serious hyperglycemic emergency in patients with diabetes. DKA has long been considered a key clinical feature of T1D. In this study, we assessed biochemical disorders focused on lipid and renal parameters in KPD.: We carried out a case-controls study for one year, encompassing 200 participants in total. Patients were outpatients newly diagnosed diabetics attending for the first time hospitals, controls were no-ketosis participants. Blood samples were taken, after 10 to 12 hours of fasting from the day before, in different tubes for each intended analyses according to instructor’s recommendation. The increased mean value of fasting blood glucose together with HbA1c are corrolated with ketonuria. As regard to cardio-metabolic risk, ketosis-prone patients were at greater risk than no-ketosis controls The mean AIP value was higher in KPD women than in men, with no significant statistical correlation. Uremia and creatininemia, two kidney parameters commonly prescribed to explore glomerular filtration, were correlated with some social indicators. The mean values of these both parameters increased significantly with age in KPD group. Creatinine increased significantly with age in patients, in line with glomerular filtration rate (GFR). The older the participants, the higher the mean values of these parameters. : These biochemical parameters, high atherogenic index and lower glomerular filtration rate, documented in our study, must be sought out when inaugural ketosis is suspected for improving the clinical prognosis of patients in the initial step of management.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"71 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139774941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-15DOI: 10.18231/j.ijcbr.2023.056
Hülya Çiçek, Abdalrhman Kuba, M. Uğur, Elif Bacak Güllü, N. Tepe
Preeclampsia is a disease characterized by hypertension, proteinuria, and edema that occurs after the 20th gestational week. It is among the most important reasons for maternal/perinatal morbidity and mortality. Although many studies have been carried out, the pathophysiology of the disease is not fully known. Many methods have been proposed for evaluating risk factors leading to preeclampsia. In the past, the methods used to predict preeclampsia have usually been focused on non-biochemical markers, but nowadays there is a shift towards biochemical markers. Recently, many biochemical agents have been started to be used in the prediction of preeclampsia. In this study, the evaluation of some serum biomarkers in the follow-up preeclampsia was aimed. Serum nesfatin, ezrin, placental protein 13, hypoxia-inducible factor 1-α subunit (HIF1A), and neuropilin 1 levels were examined with the ELISA method. In the study, 90 samples taken from subjects, including pre-treatment preeclampsia (n =35), post-treatment preeclampsia (n = 35), and healthy control (n = 20) groups were evaluated. The data obtained from the study was analyzed with SPSS 22.0. As a result of the statistical analysis, pre-treatment nesfatin-1, and ezrin levels were found significantly lower than post-treatment and the healthy control group and HIF-1A levels were found significantly higher. As a result of these analyses, pre-treatment and post-treatment PP13 levels were found to be significantly higher than the healthy control group.Considering the results obtained from the study, we can say that nestin, ezrin, HIF1A, PP13, and NRP1 are important biomarkers for predicting preeclampsia.
{"title":"Assessment of serum markers in preeclampsia: A prospective study","authors":"Hülya Çiçek, Abdalrhman Kuba, M. Uğur, Elif Bacak Güllü, N. Tepe","doi":"10.18231/j.ijcbr.2023.056","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.056","url":null,"abstract":"Preeclampsia is a disease characterized by hypertension, proteinuria, and edema that occurs after the 20th gestational week. It is among the most important reasons for maternal/perinatal morbidity and mortality. Although many studies have been carried out, the pathophysiology of the disease is not fully known. Many methods have been proposed for evaluating risk factors leading to preeclampsia. In the past, the methods used to predict preeclampsia have usually been focused on non-biochemical markers, but nowadays there is a shift towards biochemical markers. Recently, many biochemical agents have been started to be used in the prediction of preeclampsia. In this study, the evaluation of some serum biomarkers in the follow-up preeclampsia was aimed. Serum nesfatin, ezrin, placental protein 13, hypoxia-inducible factor 1-α subunit (HIF1A), and neuropilin 1 levels were examined with the ELISA method. In the study, 90 samples taken from subjects, including pre-treatment preeclampsia (n =35), post-treatment preeclampsia (n = 35), and healthy control (n = 20) groups were evaluated. The data obtained from the study was analyzed with SPSS 22.0. As a result of the statistical analysis, pre-treatment nesfatin-1, and ezrin levels were found significantly lower than post-treatment and the healthy control group and HIF-1A levels were found significantly higher. As a result of these analyses, pre-treatment and post-treatment PP13 levels were found to be significantly higher than the healthy control group.Considering the results obtained from the study, we can say that nestin, ezrin, HIF1A, PP13, and NRP1 are important biomarkers for predicting preeclampsia.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"578 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139834038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-15DOI: 10.18231/j.ijcbr.2023.060
Poonam Agrawal, Mohit Agrawal, Deepti Chopra
Gout is a metabolic disease characterised by acute or chronic arthritis which is due to deposition of Monosodium urate (MSU) crystals in joints and soft tissues. Classically described as a disease associated with hyperuricemia which may be due to metabolic defect in uric acid production or due to impaired renal excretion.We present a case of 45 year old nondiabetic, non-alcoholic, non-smoker male presented to orthopaedic OPD with complain of pain in right hand and left leg with restriction of joint mobility for past 14 hours.On carefully eliciting the dietary history patient revealed that he was a vegetarian and was on high purine rich diet for past 2 weeks due to family functions and various gatherings. Patient gave the history of consumption of cauliflower, mushrooms, beans along with sweetened carbonated drinks on two consecutive days before the onset of these symptoms.Serum uric acid 4.5 mg/dl, C-reactive protein was 9 mg/dl. To best of our knowledge this is the first case being reported in literatures where purine rich vegetarian diet has induced new case of gout.This kind of gout precipitation after consuming vegetarian diet is rarely reported in literature. This case report emphasizes upon the need of special caution to be taken by persons who are genetically predisposed to gout, while consuming purine rich diet like red meat, seafood, cauliflowers, spinach, rajma, Bengal gram, mushrooms, soft drinks sweetened with fructose.
{"title":"Vegetarian diet induced new onset acute gouty arthritis in a middle-aged genetically predisposed male: A rare presentation","authors":"Poonam Agrawal, Mohit Agrawal, Deepti Chopra","doi":"10.18231/j.ijcbr.2023.060","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.060","url":null,"abstract":"Gout is a metabolic disease characterised by acute or chronic arthritis which is due to deposition of Monosodium urate (MSU) crystals in joints and soft tissues. Classically described as a disease associated with hyperuricemia which may be due to metabolic defect in uric acid production or due to impaired renal excretion.We present a case of 45 year old nondiabetic, non-alcoholic, non-smoker male presented to orthopaedic OPD with complain of pain in right hand and left leg with restriction of joint mobility for past 14 hours.On carefully eliciting the dietary history patient revealed that he was a vegetarian and was on high purine rich diet for past 2 weeks due to family functions and various gatherings. Patient gave the history of consumption of cauliflower, mushrooms, beans along with sweetened carbonated drinks on two consecutive days before the onset of these symptoms.Serum uric acid 4.5 mg/dl, C-reactive protein was 9 mg/dl. To best of our knowledge this is the first case being reported in literatures where purine rich vegetarian diet has induced new case of gout.This kind of gout precipitation after consuming vegetarian diet is rarely reported in literature. This case report emphasizes upon the need of special caution to be taken by persons who are genetically predisposed to gout, while consuming purine rich diet like red meat, seafood, cauliflowers, spinach, rajma, Bengal gram, mushrooms, soft drinks sweetened with fructose.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"18 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139775417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-15DOI: 10.18231/j.ijcbr.2023.050
D. Trivedi, Priyali Purandare
Sickle cell anaemia is a molecular disease. WHO recognises it as a global public health problem. In India, it is common among tribal communities. Increased HbS is a culprit. Therapeutic research is focused on maintaining high levels of HbF and decreasing 2,3, BPG to target disease. To assess the role of naturally compensated haemoglobin variants in tribal Sickle cell anaemic patients of North East Gujarat. Prospective, analytical, case control study conducted on randomly selected fifty tribal Sickle cell anaemic patients having disease for more than 5 years. Fifty age and sex matched, healthy control subjects. Each fifty Tribal sickle cell anaemic patients and healthy control were included in the study. Total Haemoglobin level, Sickling test by NESTROFT method and Haemoglobin variants were analysed by alkaline haemoglobin electrophoresis. Frequencies of clinical crises were recorded by oral questioning. The results were analysed using SPSS version 20. Student unpaired t- test was employed to assess the significance of the differences. P-values < 0.05 considered statistically significant. We observed decreased levels of total haemoglobin, high levels of HbF and HbA2 along with reduced HbA0. Compensatory increase of HbF in tribal SCA patients have shown lower frequency of clinical crises. Reduced HbA0 in sickle cell anaemia is compensated by naturally elevated HbF and HbA2 in tribal patients which has a beneficial influence on their general health.
{"title":"Elevated foetal haemoglobin in tribal sickle cell anaemic patients: Blessing in disgust?","authors":"D. Trivedi, Priyali Purandare","doi":"10.18231/j.ijcbr.2023.050","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.050","url":null,"abstract":"Sickle cell anaemia is a molecular disease. WHO recognises it as a global public health problem. In India, it is common among tribal communities. Increased HbS is a culprit. Therapeutic research is focused on maintaining high levels of HbF and decreasing 2,3, BPG to target disease. To assess the role of naturally compensated haemoglobin variants in tribal Sickle cell anaemic patients of North East Gujarat. Prospective, analytical, case control study conducted on randomly selected fifty tribal Sickle cell anaemic patients having disease for more than 5 years. Fifty age and sex matched, healthy control subjects. Each fifty Tribal sickle cell anaemic patients and healthy control were included in the study. Total Haemoglobin level, Sickling test by NESTROFT method and Haemoglobin variants were analysed by alkaline haemoglobin electrophoresis. Frequencies of clinical crises were recorded by oral questioning. The results were analysed using SPSS version 20. Student unpaired t- test was employed to assess the significance of the differences. P-values < 0.05 considered statistically significant. We observed decreased levels of total haemoglobin, high levels of HbF and HbA2 along with reduced HbA0. Compensatory increase of HbF in tribal SCA patients have shown lower frequency of clinical crises. Reduced HbA0 in sickle cell anaemia is compensated by naturally elevated HbF and HbA2 in tribal patients which has a beneficial influence on their general health.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"2 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139774193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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