International Journal of Clinical Biochemistry and Research最新文献

{"title":"Study of fingerprint patterns in relation to gender and blood groups","authors":"S. Pratinidhi, Vaishali Lunawat, Mohak A. Tilokchandani, C. Bhujbal, Rutuja Shejul, Madhumita Sahoo","doi":"10.18231/j.ijcbr.2023.025","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.025","url":null,"abstract":"Dermatoglyphics (fingerprint/dactylography) is derived from the Greek word “Derma=Skin, Glyph=Carve.” Dermatoglyphics is defined as the scientific study of naturally occurring epidermal ridges and their configuration on the digits, palms and soles apart from flexion crease and secondary folds. The objective of the study was to determine correlation if any exits between the fingerprint pattern and blood group and gender.    The general study design selected for this article was cross-sectional study. Here, the primary outcome variable is to determine correlation (if any) between the fingerprint pattern and blood group and gender. A total of 271 students volunteered for the study. Loops were found to the most prevalent type of fingerprint in all blood groups. Blood groups and primary fingerprint patterns were found to be associated with the association being statistically significant. The correlation between the said entities would enable better authentication and prediction of identity of a person and thereby has the potential for great application in the field of forensics.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90160346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immune complexes in Kosali breed of cattle naturally exposed to arsenic contamination: Attenuation by","authors":"S. Dash","doi":"10.18231/j.ijcbr.2023.022","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.022","url":null,"abstract":"The study investigated the effect of bark powder on circulatory immune complexes and total immunoglobulins level in Kosali breed of cattle environmentally exposed to arsenic contamination through water and fodder. Arsenic concentration was significantly (p<0.05) higher in drinking water, fodder and cattle blood samples collected from the arsenic contaminated area as compared to uncontaminated area (control area). Kosali cattle reared in the arsenic contaminated area showed significantly (p<0.05) elevated circulatory immune complexes with decreased level of plasma total immunoglobulins compared to the control area. bark powder was administered orally @ 40 mg/kg. b. w. daily for 30 days to eight Kosali cattle (treatment group) selected from the arsenic contaminated area. Eight Kosali cattle each from arsenic contaminated and uncontaminated area (without any treatment) served as exposure control and healthy control respectively. Kosali cattle of the treatment group exhibited significant (p<0.05) decrease in the level of circulatory immune complexes; and increase in the total immunoglobulin concentration to the level comparable to that of healthy control.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78029734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of extracted DNA from whole blood and buffy coat in healthy individuals","authors":"Umesh Kumar, Khushdeep Singh, Jaspreet Singh","doi":"10.18231/j.ijcbr.2023.031","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.031","url":null,"abstract":"Deoxyribonucleic acids (DNA) are the genetic material in all organisms. Extraction of DNA is the initial gait for the field of molecular biotechnology and biomedical research to advance understanding of human illnesses from various diseases and provide practical solutions for diagnosis and treatment. Routine diagnostic techniques in medicine have been greatly enhanced by molecular biology. Due to research on deoxyribose nucleic acid, it frequently finds use in medicine. Efficient isolation of DNA from a sample is the basis for successful forensic DNA profiling. DNA can be extracted from variety of samples such as a whole blood, buffy coat, hair, skin, tissue, urine, buccal swab, saliva etc. Numerous molecular biologic applications require genomic DNA (gDNA) in high quality and in adequate quantity. We extracted genomic DNA from buffy coat and whole blood samples and compared the results of the product obtained in terms of quantity (concentration of DNA extracted and DNA obtained per ml of blood used) and quality (260nm/280nm ratio) of the obtained yield. The aim of this study was to extract DNA from whole blood and buffy coat and compare quantity or Concentration (μg/ml) and purity (260nm/280nm) of extracted DNA. The present study was a hospital- based comparative and experimental study. The study was conducted over a period of 1 year on 50 samples. The venous blood sample is taken from the healthy individuals who are willing to donate blood in blood bank. The 5 ml of blood sample taken by venipuncture from healthy individuals were equally dividing into two anticoagulant EDTA tubes by equal volume. The first tube was use as it is, as a whole blood sample and second tube were centrifuged to obtained three phase separation and prepare buffy coat. From both of the samples extraction of DNA is carried out by follow the protocols of modified salting out method. The integrity and purity of the extracted DNA are assessed by Thermo Scientific Nano Drop 2000Spectrophotometers. Our results show thatMean±SD of concentration of DNA from whole blood and buffy coat was 37.49±25.31 μg/ml and 101.83±31.22 μg/ml (P < 0.0001). The Mean±SD of purity (260nm/280nm) of DNA from whole blood and buffy coat was 1.66±0.16 and 1.74±0.15 (p≤0.05). The both p value was found to be statistically significant.The present study concluded that DNA extracted from Buffy coat had purity as well as mean concentration significantly higher when compared to DNA extracted from whole blood.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89682530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laboratory characterization of patients with chronic renal failure in resource-limited settings, with special reference to the post-COVID-19 milieu","authors":"S. Patharkar, L. Chougule, Shubhada Girish Vengurlekar, Meeta Piyush Shah, Vandana Parag","doi":"10.18231/j.ijcbr.2023.023","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.023","url":null,"abstract":"Chronic kidney disease (CKD), a known global burden involving costly diagnostic and therapeutic modalities, is rampant in urban Indian localities. This study attempts to characterize a subset of proven CKD patients attending out-patient municipal clinics and dispensaries in Mumbai, with an emphasis on the post-COVID scenario. A cross-sectional and longitudinal study was executed in 60 such subjects along with an appropriate number of normal, healthy controls, who were routinely monitored i) up to March 2020, and ii) post-April 2022, the intervening period overlapping with extremely poor and even negligible patient attendance during the COVID-19 pandemic. CKD was estimated by its two most often utilized surrogate markers, serum creatinine and blood urea nitrogen. Amongst the participants, we observed slightly increased values in the aforesaid biomarkers, the underlying aetiology of which has not, to our knowledge, been studied in detail. We recommend comprehensive additional studies in order to corroborate our findings.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"56 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80470637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum ferritin level in primigravida and multigravida women","authors":"M. C. Chandru, Y. M. Kabadi, Poonam Lalla","doi":"10.18231/j.ijcbr.2023.018","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.018","url":null,"abstract":"Iron Deficiency Anemia in pregnancy is prevalent in developing countries which can be reduced by oral iron supplement. Iron Deficiency Anemia is also the root cause for preterm birth, low birth weight and poor mental and physical development of baby. This may link to development of high blood pressure in the adult life. Hence, it is very important to diagnose the Iron Deficiency Anemia in early stage of pregnancy. Hemoglobin and serum ferritin both are apt markers for Iron Deficiency Anemia diagnosis. However, serum ferritin is considered to be the best marker over hemoglobin as hemoglobin is a delayed marker and may fail to reflect tissue iron status. In current study we have analyzed the primigravida and multigravida pregnant women with a control group to check the serum ferritin level and evaluate the Iron Deficiency Anemia status.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"139 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79318270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of cardio-vascular disease risk in diabetic population of Northern India","authors":"R. Shekhar, R. Surit, J. Keshari, P. Prakash, Sweta Kumari","doi":"10.18231/j.ijcbr.2023.019","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.019","url":null,"abstract":": Type 2 Diabetes mellitus (DM) is a chronic metabolic disorder. India has 72.9 million people living with diabetes (8.8%). The Cardio Vascular Disease (CVD) risk increases in patients with Type 2 diabetes. The Non-HDL-C level includes cholesterol carried in several atherogenic lipoproteins. The estimation of non-HDL-C may provide a better means to follow these patients toward their treatment goals. The study compares the level of non-HDL-C in non diabetic, pre-diabetic and diabetes patients and their occurrence of dyslipidemia. : The study group included 1418 patients and were grouped as – Non diabetic, pre-diabetic and Diabetic, having age group of 20 to 85 years. The Male:Female ratio was 1.9:1. Their blood samples were analysed in Clinical Biochemistry Laboratory on automated chemistry analyzer with quality checks.: The Non Diabetic group (HbA1c<5.7%) had 564 patients having Non-HDL-C 127.85±1.79 mg/dl; the Pre-Diabetic group (HbA1c-5.7-6.4%) had 316 patients with Non-HDL-C 132.37±2.35 mg/dl and Diabetic group (HbA1c >6.4%) had 538 patients and its non HDL-C was 148±4.27 mg/dl. : This study showed the groups, pre-diabetic and diabetic, had Non-HDL-C >130mg/dl. An increase in Non-HDL-C levels is associated with increased risk of CVD according to Adult Treatment Panel (ATP) III guidelines.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"47 1-2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73228000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Verification of Roche reference ranges for serum prolactin in children, adolescents, adults, and the elderly","authors":"C. Ilardo, G. Coulon, Yoann Ehrhard, Joël Barthes","doi":"10.18231/j.ijcbr.2023.024","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.024","url":null,"abstract":"Reference intervals (RIs) for prolactin are of high clinical importance for diagnosis, treatment, and prognosis of hypothalamic-pituitary disorders. The aim of this study was to verify the Roche reference ranges for serum prolactin in children, adolescents, adults, and the elderly. : An indirect method based on currently laboratory data was used on the reference ranges. Nine thousand one hundred and thirteen prolactin results were included. Pregnancy, lactation, exercise and reported pathologies were ruled out and only samples were collected between 8AM and 12PM were used. Reference ranges with a confidence interval of 95% (95% CI) were estimated. : Compared to the manufacturer's reference values and for the corresponding age group, the median values obtained in our study were 10 – 60% higher depending on the age and sex of the patients. Elevated levels of serum prolactin were observed in the neonatal period and values decrease until median values near 200 mUI/L in childhood. During the children's period, no gender differences were observed for prolactin level. The gender difference in prolactin levels became significant from pre-adolescence until the age of 60. Prolactin levels increased significantly (p<0.001) between children and young adults, followed by a gradual and continuous decrease until young senior age.: The prolactin reference values proposed by the manufacturer in the data sheet appeared unsuitable. Laboratories should review reference ranges, and a partitioning with sex and different age groups may be appropriate.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89050870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of c-reactive protein level, antioxidant and acetylcholinesterase enzyme activities among snuff users","authors":"Maryam Saeed Otuh, B. Muhammad, A. Abdullahi, Abdulkadir Hassan Lawal","doi":"10.18231/j.ijcbr.2023.029","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.029","url":null,"abstract":"The use of snuff in Nigeria especially in the North has increased alarmingly. It is no longer considered a health risk nor is it seen as morally questionable. This research was designed to investigate the effect of snuff on antioxidant activities and cognitive function on human participants. A total of 200 volunteers were divided into 4 groups based on their periods of exposure to snuff; the Control. Group 1 (1-3 years), Group 2 (3-5 years) and Group 3 (5 years and above). All these groups consist of 50 participants each. The groups were all tested for BP and Glucose levels using standard procedure before and after they were administered snuff. Blood samples were then collected. Assessments of SOD, GPx, CAT, MDA, AChE and C-reactive protein were carried out using standard methods. The result revealed a significant decrease in SOD, GPx and CAT activities and a decrease in MDA level among snuff users compared to the control. An increase in AChE activity and in C-reactive protein level were also recorded. These findings suggest that nicotine in snuff may induce oxidative stress and inflammation, and rapid degradation of acetylcholine.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87561283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A retrospective study of lipid profile during the various phases of timing in renal failure and hypothyroid patients","authors":"S. Eadala, Latha Rani Muthireddy, Durga Prasad Vangara, Srihita Mahavadi","doi":"10.18231/j.ijcbr.2023.021","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.021","url":null,"abstract":": As there are very few studies in our region about the metabolic changes in hypothyroid and renal failure patients in collection of sample during various phases of time, where these organs were involved in regulating this metabolism.To evaluate the trends of lipid profiles in hypothyroidism and chronic kidney disease throughout various phases of time. This was a retrospective control study conducted on 50 CKD patients, 50 hypothyroidism patients and 50 controls in the tertiary care hospital attending to OPD. Collected samples are analyzed for Total cholesterol, Triglycerides, and HDL cholesterol whereas LDL and VLDL are measured by calculated method taken at various phases of time. The data was entered into the master chart and analyzed statistically.: It was found that random lipid profile values in CKD and hypothyroidism were higher and are statistically significant compared to fasting and postprandial states. Particularly there is an elevation in Total cholesterol, Triglycerides, LDL cholesterol whereas HDL levels are decreased.: This research work is done by analyzing the fasting, postprandial and random states because it includes an explanation of why a random blood sample is preferred to analyze lipid profile values. Thyroid disease and renal failure patients are more prone to development of atherosclerosis which were primary cause for development of cardiovascular and cerebrovascular complications. So collection of the sample also plays key role in management.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83674337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of role of serum prolactin & ki-67 in different stages of breast cancer","authors":"P. Sagar, S Kumar, Neha Mala Krishna, Manish Kumar, J. Keshari","doi":"10.18231/j.ijcbr.2023.008","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.008","url":null,"abstract":"Breast cancer is the most frequent cancer among women. Prolactin (PRL) is a hormone secreted from anterior pituitary gland which stimulates cell proliferation, survival of cancer cells, its migration, invasion and angiogenesis. Breast cancer is strongly associated with raised plasma Prolactin level (PRL). The Prolactin behaves like Growth Hormone and its actions by the growth-promoting JAK/STAT pathway suggests its tumor-promoting effects.Ki-67 a Nuclear Protein is highest in Luminal B, Ki67 are not related to age but is correlated with tumor size. Fraction of Ki-67 positive tumor cells correlates with the clinical course of cancer cells. Hence, Ki- 67 can be an effective target in cancer therapy. Ki-67% and Serum Prolactin level may be considered a valuable biomarker in breast cancer patients and be used in treatment, follow-up and Prognosis.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85721509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}