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Metabolic engineering of Saccharomyces cerevisiae for ethanol and butanol biofuel production 乙醇和丁醇生物燃料生产中酿酒酵母的代谢工程
Pub Date : 2023-01-01 DOI: 10.22161/ijeab.82.5
Salman Khan, Asif Hussain, M. Hussain, Sahibzada Muhammad Aqeel, Samra Basharat, A. Hussain, Waleed Al-Ansi, Abdulqader M. Abdulqader, Nie Yao
The production of biofuels through biological processes has garnered increasing attention due to their potential benefits over conventional fuels, including lower greenhouse gas emissions, higher energy output, and reduced-price fluctuations. However, the metabolic processes of primitive microorganisms used in biofuel production are not compatible with those of fossil fuels. To address this, scholars have employed metabolic engineering techniques to modify the metabolisms of various microorganisms, including S. cerevisiae, for enhanced biofuel production. Specifically, overexpression of enzymes involved in bioethanol and biobutanol production, knockouts of competing pathways, improvements in carbon flux and tolerance have been applied to maximize the potential of S. cerevisiae for bioethanol and biobutanol production. This review focuses on the current state of metabolic engineering of S. cerevisiae for the production of bioethanol from lignocellulose and biobutanol from all kind of substrates, along with the potential use of cell surface technology in this field.
通过生物过程生产生物燃料已引起越来越多的关注,因为它们比传统燃料有潜在的好处,包括减少温室气体排放、提高能源产出和减少价格波动。然而,用于生物燃料生产的原始微生物的代谢过程与化石燃料的代谢过程不相容。为了解决这个问题,学者们采用代谢工程技术来修改包括酿酒酵母在内的各种微生物的代谢,以提高生物燃料的产量。具体来说,过度表达参与生物乙醇和生物丁醇生产的酶,敲除竞争途径,改善碳通量和耐受性,已经被应用于最大化酿酒酵母生产生物乙醇和生物丁醇的潜力。本文综述了酿酒酵母从木质纤维素和各种底物中生产生物乙醇的代谢工程研究现状,以及细胞表面技术在该领域的潜在应用。
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引用次数: 0
Transcriptomic analysis of Hevea brasiliensis seedlings under supplemental LED night lighting LED夜间照明下巴西橡胶树幼苗转录组学分析
Pub Date : 2023-01-01 DOI: 10.22161/ijeab.84.1
Xingcheng Yao, Hanqi Tu, Xinlong Wang, Jun Wang
Hevea brasiliensis is an important economic crop which produces natural rubber. Supplemental LED night lighting improves its growth, however the underlying molecular mechanism remains unknown. The study analyzed the transcriptome of H. brasiliensis plants under the treatment of LED night lighting. The light treatment resulted in 1047 and 411 differentially expression genes (DEGs) during the day and night time, respectively. Functional group analysis showed that DEGs in the day time enriched into 185 metabolic pathways and that DEGs in the night time enriched into 116 metabolic pathways. A total of 92 DEGs were identified between night lighting and control plants. These DEGs were involved in regulation of pigment metabolism, photosynthesis, circadian rhythm, and carbohydrate metabolism. The genes associated with circadian rhythm were altered during the day and night time. The gene involved in carbohydrate metabolic process was upregulated and the related KEGG pathways associated carbohydrate metabolism were upregulated. These results concluded that supplemental LED night lighting improve growth of hevea plants by upregulating genes associated with photosynthesis and carbohydrate metabolism, so as to synthesize more carbohydrates.
巴西橡胶树是生产天然橡胶的重要经济作物。补充LED夜间照明可促进其生长,但其潜在的分子机制尚不清楚。本研究分析了LED夜间照明下巴西香薷植株的转录组。光照处理在白天和夜间分别产生1047个和411个差异表达基因(deg)。功能组分析结果显示,白天和夜间分别富集了185条和116条代谢途径。在夜间照明和对照植物之间共鉴定出92度。这些deg参与色素代谢、光合作用、昼夜节律和碳水化合物代谢的调节。与昼夜节律相关的基因在白天和晚上都发生了改变。参与碳水化合物代谢过程的基因上调,与碳水化合物代谢相关的KEGG通路上调。综上所述,LED夜间照明通过上调与光合作用和碳水化合物代谢相关的基因,促进橡胶树的生长,从而合成更多的碳水化合物。
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引用次数: 0
Contribution of Tree Legumes in the Production Dynamics of Yellow Yam (Dioscorea cayenensis Lam) in the South Cameroon Plateau 树豆科植物对南喀麦隆高原黄山药生产动态的贡献
Pub Date : 2023-01-01 DOI: 10.22161/ijeab.82.10
Guy Aurélien Nganso Djakou
The overexploitation of agricultural soils as a result of the increased need for food by local populations is leading to serious dysfunctions in land restoration. These dysfunctions, aggravated by climate change, require the implementation of ecological engineering strategies to rehabilitate the soil. The soils of the South Cameroonian plateau suffer significant degradation in terms of loss of fertilizing elements due to extensive agricultural practices, which limits agricultural production. This work showed how tree legumes contribute to improving the yield of yellow yam (Dioscorea cayenensis) in the South Cameroonian plateau, to ultimately support the production of agroecosystems in this environment. Within the framework of this study, an experimental set-up was designed to measure the performance of tree legumes in the field. The experimental set-up is a randomized complete block trial with three treatments and three replications on 3000 m2. The control plot without shrubs, the plot with Calliandra, and the plot with Senna. This trial was carried out in the district of Mbankomo, Mefou, and Akono Department, Central Region. After monitoring the experiment, it was found that Senna spectabilis produced more nutrients than Calliandra calothyrsus. Thus, each Senna shrub produced 1.54 kg of litter in the first year and 2.04 kg in the second year for a quantity of nitrogen of 192.76 kg/ha and 260 kg/ha respectively in the first and second year. Each Calliandra shrub produced 1.46 kg and 1.87 kg of litter for a nutrient amount of 183 kg/ha and 236.81 kg/ha of nitrogen during the two years of the experiment. The combination of tree legumes and field crops increased yam yields by a factor of 2 or 3 compared to traditional agriculture. The growth rate of the yam is accelerated as well as its vegetative cycle, which is reduced from 7.5 months to 6 months as a result of the increase in soil organic matter through the shrub legume litter. Agroforestry practices could have a positive impact on soil characteristics and microbial communities, resulting in enhanced soil fertility and long-term sustainability of agricultural production. According to the results obtained, the insertion of tree legumes in agrosystems is a more efficient and less costly way to gradually and sustainably increase nitrogen availability and soil fertility.
由于当地人口对粮食的需求增加而对农业土壤的过度开发正在导致土地恢复方面的严重功能失调。这些功能失调,由于气候变化而加剧,需要实施生态工程策略来修复土壤。由于广泛的农业活动,南喀麦隆高原的土壤因肥料流失而严重退化,这限制了农业生产。这项工作显示了豆科树如何有助于提高南喀麦隆高原黄山药(Dioscorea cayenensis)的产量,并最终支持这种环境下的农业生态系统生产。在本研究的框架内,设计了一个实验装置来测量豆科树在田间的表现。实验设置为随机完全区组试验,在3000 m2上进行3个处理和3个重复。对照区无灌木、花莲区和塞纳区。这项试验是在中部地区姆班科莫、梅富和阿科诺省进行的。经实验监测发现,色番泻比花椒产生更多的营养物质。因此,在施氮量分别为192.76 kg/ha和260 kg/ha的条件下,每个塞纳灌木第一年产生1.54 kg凋落物,第二年产生2.04 kg凋落物。2年试验期间,在养分用量为183 kg/ha和氮用量为236.81 kg/ha的条件下,每株花青菜灌木凋落物产量分别为1.46 kg和1.87 kg。与传统农业相比,豆科树和大田作物的结合使山药产量增加了2到3倍。灌丛豆科凋落物增加了土壤有机质,加快了山药的生长速度,使其营养周期从7.5个月缩短到6个月。农林业实践可对土壤特征和微生物群落产生积极影响,从而提高土壤肥力和农业生产的长期可持续性。根据所获得的结果,在农业系统中插入树豆科植物是一种更有效且成本更低的方法,可以逐步和可持续地提高氮有效性和土壤肥力。
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引用次数: 0
Analysis the Potential of Malang Regency as a Center for Oil Palm Plantations in East Java 玛琅县作为东爪哇油棕种植中心的潜力分析
Pub Date : 2023-01-01 DOI: 10.22161/ijeab.84.17
K. Wicaksono, Paramyta Nila Permanasari, Akbar Saitama
East Java region is known for its good land potential for agriculture. One area that has a large enough agricultural land is Malang Regency. This feasibility study in the long run can become the foundation for establishing oil palm plantations and also a palm oil processing industry in the region. Thus, a feasibility study of various important aspects is carried out for the sustainability and sustainability of the development of the oil palm plantation industry in Malang. The purpose of this study is to see how land and environmental conditions suitability and to study and map the suitability of oil palm land in Malang Regency. The study was carried out in October - December 2021 in the southern Malang region and the southern Blitar location as a comparison area and industrial survey to find out data on oil quality in the southern region of Malang Regency and Blitar. Studies use survey methods to describe situations and events with the aim of solving problems in a systematic, fluctuating and accurate manner regarding the facts and characteristics of a particular population or area. Land suitability variables are in the form of geographic data, such as slopes and determination of points; macro climate data and micro climate data, such as radiation intensity, RH, temperature, flood potential and soil data including physical properties, such as soil texture, structure and depth; biological properties, such as c-organic content, litter thickness and presence of earthworms and chemical properties, such as analysis of N, P and K content, pH and base saturation and soil CEC. Based on a comparison of soil analysis data in the areas of Malang, Sumatra and Kalimantan, it can be said that the land suitability of Malang Regency is in accordance with the needs of oil palm plants based on land suitability.
东爪哇地区以其良好的农业用地潜力而闻名。一个拥有足够大的农业用地的地区是玛琅摄政。从长远来看,这项可行性研究可以成为在该地区建立油棕种植园和棕榈油加工业的基础。因此,对玛琅油棕种植业的可持续性和可持续性发展进行了各个重要方面的可行性研究。本研究的目的是了解土地和环境条件的适宜性,并研究和绘制玛琅摄政油棕土地的适宜性。该研究于2021年10月至12月在Malang南部地区和Blitar南部地区进行,作为比较区和工业调查,以找出Malang Regency和Blitar南部地区的石油质量数据。研究使用调查方法来描述情况和事件,目的是以系统、波动和准确的方式解决有关特定人口或地区的事实和特征的问题。土地适宜性变量以地理数据的形式存在,例如坡度和点的确定;宏观气候数据和微观气候数据,如辐射强度、相对湿度、温度、洪势和土壤数据(包括土壤质地、结构和深度等物理性质);生物特性,如碳有机含量、凋落物厚度和蚯蚓的存在;化学特性,如N、P、K含量、pH、碱饱和度和土壤CEC分析。通过对玛琅、苏门答腊岛和加里曼丹地区土壤分析数据的对比,可以说玛琅摄政的土地适宜性是按照油棕植物在土地适宜性基础上的需要进行的。
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引用次数: 0
Air pollution in Bamako: Modeling, Pollution-Population Index 巴马科的空气污染:建模,污染-人口指数
Pub Date : 2023-01-01 DOI: 10.22161/ijeab.85.5
Yacouba Maiga, Bankoro Bagayoko, Hady Diallo, Boubacar Kola Touré, Sékou Sako, Ousmane Coulibaly, Ibrahima Yattara, Mohamed Maiga
This study on air pollution in Bamako carried out in 2019, aims to characterize the different sources of emissions, model the health impact of ambient air quality for the different scenarios, the proposal of an action plan to limit emissions, and the simulation of the evolution of emissions and ambient air quality by 2015 and 2020 with and without an action plan. The results of the two air quality measurement campaigns show concentration levels lower or substantially equal between those of July 2019 and those of the 2009 data. The PM10 concentration recorded indicates a significant excess area compared to the values concentration guides set by the WHO. Analysis of the evaluation of emissions and their health impact by 2015 and 2020 shows that pollution by volatile organic compounds and dust will become unacceptable if nothing is done. The number of cancers linked to benzene will increase to 686 cases and the increase in mortality due to dust to an average figure of 38.7%.
这项针对巴马科空气污染的研究于2019年开展,旨在描述不同排放源的特征,模拟不同情景下环境空气质量对健康的影响,提出限制排放的行动计划,并模拟到2015年和2020年在有无行动计划的情况下排放和环境空气质量的演变。两次空气质量测量活动的结果显示,2019年7月的浓度水平与2009年的数据相比更低或基本相等。与世界卫生组织设定的浓度指南值相比,记录的PM10浓度显示出明显超标的区域。到2015年和2020年对排放及其健康影响的评估分析表明,如果不采取任何措施,挥发性有机化合物和粉尘的污染将变得不可接受。与苯有关的癌症病例将增加到686例,因粉尘造成的死亡率平均增加到38.7%。
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引用次数: 0
Assessment of the Nutritional Content and Hedonic Test on Pameling Avocado from Three Different Altitudes 不同海拔地区Pameling牛油果的营养成分评价及欣赏性试验
Pub Date : 2023-01-01 DOI: 10.22161/ijeab.85.6
Paramyta Nila Permanasari, Anna Satyana Karyawati, Akbar Saitama, Karuniawan Puji Wicaksono
Malang Regency has superior avocado commodity which is so-called Pameling. Superiorities of the fruit are indicated by the large size of the fruit and quality of the fruit flesh which is liked by the consumers. Today, the development of Pameling avocado has expanded from the lowlands to the highlands in diverse regions. In order to obtain optimal harvest yields, both quantity and quality, the research affirmed the quality of Pameling avocado, which is grown at the lowlands and the highlands. The research was conducted by testing quality (nutritional content) and organoleptic. Results of the research showed that the avocados grown at the highlands were more preferred by the panelists based on the organoleptic test from the assessment aspects of appearance, texture, aroma, and taste. The lowlands produced preference of the color flesh which is mostly preferred by the panelists. Results of the nutritional test (quality) on fruits grown at the lowlands showed the highest level of sugar and protein. The middle plains are superior in fat content. Moreover, the highlands produce fruits with the highest coarse fibers. However, the differences in cultivation methods on three different altitudes will be the subject of further studies that need to be considered.
玛琅摄政有优质的牛油果商品,被称为Pameling。其优势在于果实体积大,果肉质量好,深受消费者喜爱。今天,帕美林鳄梨的发展已经从低地扩展到不同地区的高地。为了获得最优的收获产量,在数量和质量上,本研究对种植在低地和高地的Pameling牛油果的品质进行了肯定。通过质量(营养成分)和感官测试进行了研究。研究结果表明,通过感官测试,从外观、质地、香气和口感四个方面评估,高原鳄梨更受小组成员的青睐。低地产生了对肉色的偏好,这是小组成员最喜欢的。对生长在低地的水果进行营养(品质)测试的结果表明,其糖和蛋白质含量最高。中部平原地区的脂肪含量较高。此外,高地出产的水果含有最高的粗纤维。然而,三种不同海拔地区种植方法的差异将是进一步研究的主题,需要考虑。
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引用次数: 0
Yield Modeling of Okra (Abelmoschus esculentus L. moench) in Bituminous Soils of Southern Ondo State, Nigeria 尼日利亚南部翁多州沥青土中秋葵产量模型研究
Pub Date : 2023-01-01 DOI: 10.22161/ijeab.85.13
Imoukhuede Oladunni Bimpe, Fasinmirin Johnson Toyin, Olayanju Gbenga Moses
This study investigated the growth and yield response of okra (Abelmoschus esculentus L. Moench) in bituminous soils of selected communities (Loda, Lofo and Legbogbo) in Irele, southern Ondo State for two consecutive growing seasons (2020 and 2021). The Legbogbo site, which of very low bitumen, serves as the control. The textural class is sandy loam and the soil is acidic. Some samples of soil were collected from three dug soil profile pit at depths 0-100 cm at an interval of 10 cm and moved to the soil laboratory for the analysis of the chemical concentrations of macro and trace elements. The highest value of the heavy metal was recorded at Loda. More so, the least amount of the bitumen concentration was recorded at Legbogbo site (control) and in some cases at Lofo site. Result from the study showed that there is significant difference (P < 0.05) in the values of the heavy metals among the location at all depths. The results from various locations also showed that the soil depth and location were significant (P < 0.05) in their main effects on the soil heavy metals. It was observed that the heavy metal concentration of soil was lower than the permissible limit, an indication that the concentration of heavy metals of the study areas may not have negatively influenced the growth and yield of okra. All agronomic parameters in all locations during the two seasons of experiment were in the way of Legbogbo>Lofo>Loda. Okra had 0 kg.ha-1 pod yield at the Loda site at both 2020 and 2021 growing seasons, while Legbogbo had the greatest fruit yield for both seasons. Okra yield prediction model was developed using the stepwise regression model and it was observed that Okra yield significantly correlated to potassium (r2 = 0.95) at P < 0.05 and phosphorus (r2 = 0.99) (P < 0.01).
本研究连续两个生长季节(2020年和2021年)在Ondo州南部irelle选择群落(Loda、Lofo和Legbogbo)的沥青土中调查了秋葵(Abelmoschus esculentus L. Moench)的生长和产量响应。Legbogbo的沥青含量非常低,作为对照。质地类为砂质壤土,土壤呈酸性。在深度0 ~ 100 cm处挖3个土壤剖面坑,每隔10 cm采集部分土壤样品,移至土壤实验室进行宏量元素和微量元素的化学浓度分析。重金属含量最高的地区是罗达。更重要的是,在Legbogbo站点(对照)和Lofo站点的某些情况下,沥青浓度最低。研究结果表明,两者存在显著差异(P <各深度位置间重金属值差异0.05)。不同地点的结果也表明,土壤深度和位置具有显著性(P <0.05)对土壤重金属的主要影响。土壤重金属浓度低于允许的极限值,表明研究区重金属浓度可能没有对秋葵的生长和产量产生负面影响。试验两季各地点的所有农艺参数均为Legbogbo>Lofo>Loda。秋葵体重0公斤。Loda站点在2020和2021生长季的豆荚产量均为ha-1,而Legbogbo站点在两个生长季的果实产量均最高。利用逐步回归模型建立秋葵产量预测模型,发现在P <下,秋葵产量与钾含量呈显著相关(r2 = 0.95);0.05和磷(r2 = 0.99) (P <0.01)。
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引用次数: 0
Analysis of the Potential Horticultural Products in Blitar Regency on the Agribusiness Market in Blitar Regency 英国农业综合经营市场潜力园艺产品分析
Pub Date : 2023-01-01 DOI: 10.22161/ijeab.85.7
Karuniawan Puji Wicaksono, Paramyta Nila Permanasari, Akbar Saitama
Blitar Regency has a wholesale market managed by the Wlingi Market Association, but some horticultural products from the farmers are still absorbed by the wholesale market outside Blitar Regency and reabsorbed by the retailers from Blitar Regency. Objectives of the research were to obtain information about the superior products, supply chain, and the inhibiting factors on the development of the horticultural products. Method of the research used descriptive analysis on each member of the links. Location of the research is the wholesale market in Blitar Regency, Wlingi. Variables of the research include types, volumes, and circulation of the horticultural products. Results of the research showed 3 major horticultural commodities that include chili, red pepper, and watermelon. The potential agricultural products in Blitar Regency have inefficient and complex supply chain because they involve many actors from farmers to consumers. The inhibiting factors are as follow: 1. The farmers do not have a collective system for marketing their crops so that information about the products as well as the prices are not integrated, 2. The supply chain patterns are inefficient and complex, so that the prices are costly, and 3. Inadequate location of the wholesale market. It is expected that the approach of supply chain on horticultural commodities in Blitar Regency will provide an overview of potential commodity supply availability as a consideration for supply chain management.
Blitar Regency有一个由Wlingi市场协会管理的批发市场,但是一些来自农民的园艺产品仍然被Blitar Regency外的批发市场吸收,并被Blitar Regency的零售商重新吸收。本研究旨在了解我国园艺产品发展的优势产品、供应链及制约因素。本研究采用描述性分析的方法对各成员的联系进行分析。研究地点是Wlingi Blitar Regency的批发市场。研究的变量包括园艺产品的类型、数量和流通。研究结果显示,3种主要的园艺商品包括辣椒、红辣椒和西瓜。由于涉及从农民到消费者的众多行为体,英国潜在农产品的供应链效率低下且复杂。抑制因素如下:1.抑制因素;农民没有一个集体的系统来销售他们的作物,所以关于产品和价格的信息没有整合。2 .供应链模式低效复杂,价格昂贵;批发市场位置不合理。预计在英国,园艺商品供应链的方法将提供潜在商品供应可用性的概述,作为供应链管理的考虑因素。
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引用次数: 0
Experimental study of the characterization of household waste in Bamako, Mali 马里巴马科城市生活垃圾特性的实验研究
Pub Date : 2023-01-01 DOI: 10.22161/ijeab.82.17
Mohamadou Adamou, M. Maiga, O. Cissé, J. Sagara, Yaya Ouattara
In Bamako, the flow of household waste and its typology are little known. In order to set up a sustainable management system for the city's garbage, it is nevertheless important to know the quantities, natures and dimensions of the objects that make up the garbage. This study, which began in 2017, consisted of characterizing the garbage collected at the household level in Bamako using the MODECOM method modified to adapt it to the Malian context. The objective of this study was to propose a method intended to obtain solid results on the characteristics of household waste produced in Bamako in order to build adapted collection and recovery strategies. The results, obtained from a sample of 270 households in the 6 communes of Bamako and 3 neighborhoods of different socio-economic levels per commune, showed that the average ratio of household waste produced in Bamako is about 0.28 Kg per inhabitant per day. The category of fines is the most important, with an average daily production of 2.45 kg for a household with an average of 20.5 inhabitants. Putrescibles come in second place. Their production represents 41% of the average amount of garbage generated in a high standard household. It represents the main biodegradable category of garbage. Fines, which are mostly sand, charcoal and ash, accounted for 37 percent of the waste generated by households of the same type. The particle size sorting, which can help to choose suitable industrial sorting tools, shows that the fraction of medium-sized garbage is the most abundant. The data from the characterization of garbage would allow the development of all forms of garbage management strategies, especially for its collection and recovery.
在巴马科,人们对家庭垃圾的流动及其类型知之甚少。然而,为了建立一个可持续的城市垃圾管理系统,了解构成垃圾的物体的数量、性质和尺寸是很重要的。这项研究始于2017年,包括使用经过修改的MODECOM方法对巴马科家庭收集的垃圾进行特征描述,以使其适应马里的环境。这项研究的目的是提出一种方法,旨在就巴马科产生的家庭废物的特征取得可靠的结果,以便制定适当的收集和回收战略。从巴马科6个社区的270户家庭和每个社区不同社会经济水平的3个社区的样本中获得的结果表明,巴马科的家庭垃圾平均产生率约为每人每天0.28公斤。罚款是最重要的,一个平均20.5人的家庭平均每天的产量为2.45公斤。腐尸排在第二位。它们的产量占一个高标准家庭平均垃圾产生量的41%。它代表了主要的可生物降解垃圾类别。颗粒主要是沙子、木炭和灰烬,占同类型家庭产生的废物的37%。粒径分选结果表明,中型垃圾的分选比例最为丰富,有助于选择合适的工业分选工具。对垃圾进行定性分析所得的数据将有助于制定各种形式的垃圾管理战略,特别是垃圾的收集和回收。
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引用次数: 0
Microbial Assessment of Solid Waste and Bioaerosol Associated with Open Dumping Sites of the Kathmandu City, Nepal 与尼泊尔加德满都市露天倾倒场有关的固体废物和生物气溶胶的微生物评估
Pub Date : 2023-01-01 DOI: 10.22161/ijeab.83.7
Dibyak Kapali, Elisha Shakya, D. Pradhan, Elina Sigdel, Jyotin Shah, S. Thapaliya
The study aims to isolate and identify bacteria and fungi (mold) present in solid waste and its associated bioaerosol in the Kathmandu city. A total of 10 samples; 5 different solid waste samples and 5 different bioaerosol samples, collected from 5 open dumping sites in the Kathmandu city, were transported to the microbiology laboratory of St. Xavier’s College for processing. Standard microbiological procedures were followed for the identification of isolates. The Kirby-Bauer disk diffusion method was used to determine the antibiotic susceptibility of bacterial isolates following CLSI 2020 standards. In the collected solid waste samples, the bacterial colony count ranged from 1.27 × 108 to 2.8 × 108 CFU/ml, whereas the fungi colony count ranged from 1 × 105 to 4 × 105 CFU/ml. Bacterial colony counts from bioaerosol samples ranged from 116 to >300 CFU/90mm/15 minutes, whereas fungi colony counts were between 2 and 6 CFU/90mm/15 minutes. Out of 48 bacteria and 34 molds identified, Bacillus spp. (27%) and Aspergillus niger (29%) were found to be predominant than other isolates. Citrobacter spp., Salmonella spp., and Escherichia coli isolated from solid waste samples of dump site S3 showed maximum resistance to the different antibiotics used. The common microbial isolates from solid waste samples and bioaerosol samples included 7 different bacteria and 4 different molds. The presence of antibiotic-resistant bacteria and pathogenic fungi in waste dump sites pose public health-related risks.
该研究旨在分离和鉴定加德满都城市固体废物及其相关生物气溶胶中存在的细菌和真菌(霉菌)。共10个样本;从加德满都市5个露天垃圾场收集的5个不同固体废物样本和5个不同生物气溶胶样本被运送到圣泽维尔学院微生物实验室进行处理。按照标准微生物学程序鉴定分离物。采用Kirby-Bauer纸片扩散法对符合CLSI 2020标准的分离菌进行药敏试验。在所收集的固体废物样品中,细菌菌落计数范围为1.27 × 108 ~ 2.8 × 108 CFU/ml,真菌菌落计数范围为1 × 105 ~ 4 × 105 CFU/ml。生物气溶胶样品的细菌菌落计数范围为116至300 CFU/90mm/15分钟,而真菌菌落计数范围为2至6 CFU/90mm/15分钟。在鉴定的48种细菌和34种霉菌中,芽孢杆菌(27%)和黑曲霉(29%)占优势。从S3垃圾场固体废物样品中分离出的柠檬酸杆菌、沙门氏菌和大肠杆菌对不同抗生素的耐药性最大。固体废弃物样品和生物气溶胶样品中常见的微生物分离物包括7种不同的细菌和4种不同的霉菌。垃圾场中抗生素耐药细菌和致病真菌的存在构成公共卫生风险。
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引用次数: 0
期刊
International Journal of Environment, Agriculture and Biotechnology
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