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Contribution of Tree Legumes in the Production Dynamics of Yellow Yam (Dioscorea cayenensis Lam) in the South Cameroon Plateau 树豆科植物对南喀麦隆高原黄山药生产动态的贡献
Pub Date : 2023-01-01 DOI: 10.22161/ijeab.82.10
Guy Aurélien Nganso Djakou
The overexploitation of agricultural soils as a result of the increased need for food by local populations is leading to serious dysfunctions in land restoration. These dysfunctions, aggravated by climate change, require the implementation of ecological engineering strategies to rehabilitate the soil. The soils of the South Cameroonian plateau suffer significant degradation in terms of loss of fertilizing elements due to extensive agricultural practices, which limits agricultural production. This work showed how tree legumes contribute to improving the yield of yellow yam (Dioscorea cayenensis) in the South Cameroonian plateau, to ultimately support the production of agroecosystems in this environment. Within the framework of this study, an experimental set-up was designed to measure the performance of tree legumes in the field. The experimental set-up is a randomized complete block trial with three treatments and three replications on 3000 m2. The control plot without shrubs, the plot with Calliandra, and the plot with Senna. This trial was carried out in the district of Mbankomo, Mefou, and Akono Department, Central Region. After monitoring the experiment, it was found that Senna spectabilis produced more nutrients than Calliandra calothyrsus. Thus, each Senna shrub produced 1.54 kg of litter in the first year and 2.04 kg in the second year for a quantity of nitrogen of 192.76 kg/ha and 260 kg/ha respectively in the first and second year. Each Calliandra shrub produced 1.46 kg and 1.87 kg of litter for a nutrient amount of 183 kg/ha and 236.81 kg/ha of nitrogen during the two years of the experiment. The combination of tree legumes and field crops increased yam yields by a factor of 2 or 3 compared to traditional agriculture. The growth rate of the yam is accelerated as well as its vegetative cycle, which is reduced from 7.5 months to 6 months as a result of the increase in soil organic matter through the shrub legume litter. Agroforestry practices could have a positive impact on soil characteristics and microbial communities, resulting in enhanced soil fertility and long-term sustainability of agricultural production. According to the results obtained, the insertion of tree legumes in agrosystems is a more efficient and less costly way to gradually and sustainably increase nitrogen availability and soil fertility.
由于当地人口对粮食的需求增加而对农业土壤的过度开发正在导致土地恢复方面的严重功能失调。这些功能失调,由于气候变化而加剧,需要实施生态工程策略来修复土壤。由于广泛的农业活动,南喀麦隆高原的土壤因肥料流失而严重退化,这限制了农业生产。这项工作显示了豆科树如何有助于提高南喀麦隆高原黄山药(Dioscorea cayenensis)的产量,并最终支持这种环境下的农业生态系统生产。在本研究的框架内,设计了一个实验装置来测量豆科树在田间的表现。实验设置为随机完全区组试验,在3000 m2上进行3个处理和3个重复。对照区无灌木、花莲区和塞纳区。这项试验是在中部地区姆班科莫、梅富和阿科诺省进行的。经实验监测发现,色番泻比花椒产生更多的营养物质。因此,在施氮量分别为192.76 kg/ha和260 kg/ha的条件下,每个塞纳灌木第一年产生1.54 kg凋落物,第二年产生2.04 kg凋落物。2年试验期间,在养分用量为183 kg/ha和氮用量为236.81 kg/ha的条件下,每株花青菜灌木凋落物产量分别为1.46 kg和1.87 kg。与传统农业相比,豆科树和大田作物的结合使山药产量增加了2到3倍。灌丛豆科凋落物增加了土壤有机质,加快了山药的生长速度,使其营养周期从7.5个月缩短到6个月。农林业实践可对土壤特征和微生物群落产生积极影响,从而提高土壤肥力和农业生产的长期可持续性。根据所获得的结果,在农业系统中插入树豆科植物是一种更有效且成本更低的方法,可以逐步和可持续地提高氮有效性和土壤肥力。
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引用次数: 0
Analysis the Potential of Malang Regency as a Center for Oil Palm Plantations in East Java 玛琅县作为东爪哇油棕种植中心的潜力分析
Pub Date : 2023-01-01 DOI: 10.22161/ijeab.84.17
K. Wicaksono, Paramyta Nila Permanasari, Akbar Saitama
East Java region is known for its good land potential for agriculture. One area that has a large enough agricultural land is Malang Regency. This feasibility study in the long run can become the foundation for establishing oil palm plantations and also a palm oil processing industry in the region. Thus, a feasibility study of various important aspects is carried out for the sustainability and sustainability of the development of the oil palm plantation industry in Malang. The purpose of this study is to see how land and environmental conditions suitability and to study and map the suitability of oil palm land in Malang Regency. The study was carried out in October - December 2021 in the southern Malang region and the southern Blitar location as a comparison area and industrial survey to find out data on oil quality in the southern region of Malang Regency and Blitar. Studies use survey methods to describe situations and events with the aim of solving problems in a systematic, fluctuating and accurate manner regarding the facts and characteristics of a particular population or area. Land suitability variables are in the form of geographic data, such as slopes and determination of points; macro climate data and micro climate data, such as radiation intensity, RH, temperature, flood potential and soil data including physical properties, such as soil texture, structure and depth; biological properties, such as c-organic content, litter thickness and presence of earthworms and chemical properties, such as analysis of N, P and K content, pH and base saturation and soil CEC. Based on a comparison of soil analysis data in the areas of Malang, Sumatra and Kalimantan, it can be said that the land suitability of Malang Regency is in accordance with the needs of oil palm plants based on land suitability.
东爪哇地区以其良好的农业用地潜力而闻名。一个拥有足够大的农业用地的地区是玛琅摄政。从长远来看,这项可行性研究可以成为在该地区建立油棕种植园和棕榈油加工业的基础。因此,对玛琅油棕种植业的可持续性和可持续性发展进行了各个重要方面的可行性研究。本研究的目的是了解土地和环境条件的适宜性,并研究和绘制玛琅摄政油棕土地的适宜性。该研究于2021年10月至12月在Malang南部地区和Blitar南部地区进行,作为比较区和工业调查,以找出Malang Regency和Blitar南部地区的石油质量数据。研究使用调查方法来描述情况和事件,目的是以系统、波动和准确的方式解决有关特定人口或地区的事实和特征的问题。土地适宜性变量以地理数据的形式存在,例如坡度和点的确定;宏观气候数据和微观气候数据,如辐射强度、相对湿度、温度、洪势和土壤数据(包括土壤质地、结构和深度等物理性质);生物特性,如碳有机含量、凋落物厚度和蚯蚓的存在;化学特性,如N、P、K含量、pH、碱饱和度和土壤CEC分析。通过对玛琅、苏门答腊岛和加里曼丹地区土壤分析数据的对比,可以说玛琅摄政的土地适宜性是按照油棕植物在土地适宜性基础上的需要进行的。
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引用次数: 0
Alarm: Mankind is Not Ready for Inevitable Global Climate Change 警报:人类还没有为不可避免的全球气候变化做好准备
Pub Date : 2023-01-01 DOI: 10.22161/ijeab.81.11
Dr. Mikhail Krasnyansky
This article is a scientific review, first, of the consequences of global warming and climate change, and second, of the urgent ways for humanity to adapt to these climate changes. It is shown that an increase in the concentration of CO2 in the atmosphere, as well as an increase in its average temperature, correlates well with an increase in the number of natural disasters and, accordingly, an increase in budget costs for the removal of their consequences. On the other hand, the impossibility of completely stopping global climate change, let alone returning to its 1970 parameters, is discussed, for example: removing billions of tons of CO2 from the Earth's atmosphere; restoring trillions of tons of ice at the poles of the Earth and its mountain peaks; reduce the acidity of the waters of the World Ocean and its cooling. Hence the conclusion is made about the inexpediency of spending hundreds of billions of dollars on "the fight against climate change" and the expediency of spending these billions on the urgent adaptation of mankind to an already changed climate. Further, the article examines in detail the ways and options to prevent the destruction and loss caused by hurricanes, flooding, wildfires, massive burning of fossil fuels, traditional agriculture, as well as huge pollution of the World Ocean: replacement of fossil fuels with green energy and green transport, the construction of only hurricane-resistant and fire-resistant buildings in hazardous areas, the construction of dams and powerful drainage systems in areas of probable flooding, the transition to superconducting underground electrical cables, the widespread use of small modular low-enriched uranium nuclear reactors, widespread use of heat pumps, the transition from traditional cattle breeding to artificial meat, the widespread in agriculture use of so-called vertical farms, minimization of food losses at all stages, and more. The main idea of this report was clearly expressed by Charles Darwin in his well-known quote: “It is not the strongest and smartest who survive but the one who adjusts best to the changing environment.” That is, not the strongest nation (with the largest GDP), not the smartest nation (which will learn to extract all the excess CO2 from the atmosphere), but the nation that is best adapted to live in this changed climate (with hurricanes, floods, droughts, wildfires, etc.) - will have more chances to survive.
这篇文章是一篇科学综述,首先是关于全球变暖和气候变化的后果,其次是人类适应这些气候变化的紧迫方法。报告显示,大气中二氧化碳浓度的增加及其平均温度的增加与自然灾害次数的增加密切相关,因此,消除其后果的预算费用也会增加。另一方面,讨论了完全阻止全球气候变化的不可能性,更不用说回到1970年的参数,例如:从地球大气中去除数十亿吨二氧化碳;恢复地球两极和山峰上数万亿吨的冰;减少世界海洋海水的酸度和冷却。因此,人们得出的结论是,把数千亿美元花在“对抗气候变化”上是不明智的,而把这数十亿美元花在人类对已经发生变化的气候的紧急适应上是明智的。此外,文章详细探讨了防止飓风、洪水、野火、大量燃烧化石燃料、传统农业以及世界海洋的巨大污染所造成的破坏和损失的方法和选择:用绿色能源和绿色交通替代化石燃料,在危险地区只建造抗飓风和防火的建筑物,在可能发生洪水的地区建造水坝和强大的排水系统,向超导地下电缆过渡,广泛使用小型模块化低浓缩铀核反应堆,广泛使用热泵,从传统的养牛向人造肉过渡,在农业中广泛使用所谓的垂直农场,最大限度地减少各个阶段的粮食损失,等等。查尔斯·达尔文的名言清楚地表达了这份报告的主要思想:“生存下来的不是最强壮和最聪明的人,而是最能适应不断变化的环境的人。”也就是说,不是最强大的国家(拥有最大的GDP),不是最聪明的国家(将学会从大气中提取所有多余的二氧化碳),而是最适应这种变化的气候(飓风,洪水,干旱,野火等)的国家-将有更多的生存机会。
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引用次数: 0
Transcriptomic analysis of Hevea brasiliensis seedlings under supplemental LED night lighting LED夜间照明下巴西橡胶树幼苗转录组学分析
Pub Date : 2023-01-01 DOI: 10.22161/ijeab.84.1
Xingcheng Yao, Hanqi Tu, Xinlong Wang, Jun Wang
Hevea brasiliensis is an important economic crop which produces natural rubber. Supplemental LED night lighting improves its growth, however the underlying molecular mechanism remains unknown. The study analyzed the transcriptome of H. brasiliensis plants under the treatment of LED night lighting. The light treatment resulted in 1047 and 411 differentially expression genes (DEGs) during the day and night time, respectively. Functional group analysis showed that DEGs in the day time enriched into 185 metabolic pathways and that DEGs in the night time enriched into 116 metabolic pathways. A total of 92 DEGs were identified between night lighting and control plants. These DEGs were involved in regulation of pigment metabolism, photosynthesis, circadian rhythm, and carbohydrate metabolism. The genes associated with circadian rhythm were altered during the day and night time. The gene involved in carbohydrate metabolic process was upregulated and the related KEGG pathways associated carbohydrate metabolism were upregulated. These results concluded that supplemental LED night lighting improve growth of hevea plants by upregulating genes associated with photosynthesis and carbohydrate metabolism, so as to synthesize more carbohydrates.
巴西橡胶树是生产天然橡胶的重要经济作物。补充LED夜间照明可促进其生长,但其潜在的分子机制尚不清楚。本研究分析了LED夜间照明下巴西香薷植株的转录组。光照处理在白天和夜间分别产生1047个和411个差异表达基因(deg)。功能组分析结果显示,白天和夜间分别富集了185条和116条代谢途径。在夜间照明和对照植物之间共鉴定出92度。这些deg参与色素代谢、光合作用、昼夜节律和碳水化合物代谢的调节。与昼夜节律相关的基因在白天和晚上都发生了改变。参与碳水化合物代谢过程的基因上调,与碳水化合物代谢相关的KEGG通路上调。综上所述,LED夜间照明通过上调与光合作用和碳水化合物代谢相关的基因,促进橡胶树的生长,从而合成更多的碳水化合物。
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引用次数: 0
Air pollution in Bamako: Modeling, Pollution-Population Index 巴马科的空气污染:建模,污染-人口指数
Pub Date : 2023-01-01 DOI: 10.22161/ijeab.85.5
Yacouba Maiga, Bankoro Bagayoko, Hady Diallo, Boubacar Kola Touré, Sékou Sako, Ousmane Coulibaly, Ibrahima Yattara, Mohamed Maiga
This study on air pollution in Bamako carried out in 2019, aims to characterize the different sources of emissions, model the health impact of ambient air quality for the different scenarios, the proposal of an action plan to limit emissions, and the simulation of the evolution of emissions and ambient air quality by 2015 and 2020 with and without an action plan. The results of the two air quality measurement campaigns show concentration levels lower or substantially equal between those of July 2019 and those of the 2009 data. The PM10 concentration recorded indicates a significant excess area compared to the values concentration guides set by the WHO. Analysis of the evaluation of emissions and their health impact by 2015 and 2020 shows that pollution by volatile organic compounds and dust will become unacceptable if nothing is done. The number of cancers linked to benzene will increase to 686 cases and the increase in mortality due to dust to an average figure of 38.7%.
这项针对巴马科空气污染的研究于2019年开展,旨在描述不同排放源的特征,模拟不同情景下环境空气质量对健康的影响,提出限制排放的行动计划,并模拟到2015年和2020年在有无行动计划的情况下排放和环境空气质量的演变。两次空气质量测量活动的结果显示,2019年7月的浓度水平与2009年的数据相比更低或基本相等。与世界卫生组织设定的浓度指南值相比,记录的PM10浓度显示出明显超标的区域。到2015年和2020年对排放及其健康影响的评估分析表明,如果不采取任何措施,挥发性有机化合物和粉尘的污染将变得不可接受。与苯有关的癌症病例将增加到686例,因粉尘造成的死亡率平均增加到38.7%。
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引用次数: 0
Yield Modeling of Okra (Abelmoschus esculentus L. moench) in Bituminous Soils of Southern Ondo State, Nigeria 尼日利亚南部翁多州沥青土中秋葵产量模型研究
Pub Date : 2023-01-01 DOI: 10.22161/ijeab.85.13
Imoukhuede Oladunni Bimpe, Fasinmirin Johnson Toyin, Olayanju Gbenga Moses
This study investigated the growth and yield response of okra (Abelmoschus esculentus L. Moench) in bituminous soils of selected communities (Loda, Lofo and Legbogbo) in Irele, southern Ondo State for two consecutive growing seasons (2020 and 2021). The Legbogbo site, which of very low bitumen, serves as the control. The textural class is sandy loam and the soil is acidic. Some samples of soil were collected from three dug soil profile pit at depths 0-100 cm at an interval of 10 cm and moved to the soil laboratory for the analysis of the chemical concentrations of macro and trace elements. The highest value of the heavy metal was recorded at Loda. More so, the least amount of the bitumen concentration was recorded at Legbogbo site (control) and in some cases at Lofo site. Result from the study showed that there is significant difference (P < 0.05) in the values of the heavy metals among the location at all depths. The results from various locations also showed that the soil depth and location were significant (P < 0.05) in their main effects on the soil heavy metals. It was observed that the heavy metal concentration of soil was lower than the permissible limit, an indication that the concentration of heavy metals of the study areas may not have negatively influenced the growth and yield of okra. All agronomic parameters in all locations during the two seasons of experiment were in the way of Legbogbo>Lofo>Loda. Okra had 0 kg.ha-1 pod yield at the Loda site at both 2020 and 2021 growing seasons, while Legbogbo had the greatest fruit yield for both seasons. Okra yield prediction model was developed using the stepwise regression model and it was observed that Okra yield significantly correlated to potassium (r2 = 0.95) at P < 0.05 and phosphorus (r2 = 0.99) (P < 0.01).
本研究连续两个生长季节(2020年和2021年)在Ondo州南部irelle选择群落(Loda、Lofo和Legbogbo)的沥青土中调查了秋葵(Abelmoschus esculentus L. Moench)的生长和产量响应。Legbogbo的沥青含量非常低,作为对照。质地类为砂质壤土,土壤呈酸性。在深度0 ~ 100 cm处挖3个土壤剖面坑,每隔10 cm采集部分土壤样品,移至土壤实验室进行宏量元素和微量元素的化学浓度分析。重金属含量最高的地区是罗达。更重要的是,在Legbogbo站点(对照)和Lofo站点的某些情况下,沥青浓度最低。研究结果表明,两者存在显著差异(P <各深度位置间重金属值差异0.05)。不同地点的结果也表明,土壤深度和位置具有显著性(P <0.05)对土壤重金属的主要影响。土壤重金属浓度低于允许的极限值,表明研究区重金属浓度可能没有对秋葵的生长和产量产生负面影响。试验两季各地点的所有农艺参数均为Legbogbo>Lofo>Loda。秋葵体重0公斤。Loda站点在2020和2021生长季的豆荚产量均为ha-1,而Legbogbo站点在两个生长季的果实产量均最高。利用逐步回归模型建立秋葵产量预测模型,发现在P <下,秋葵产量与钾含量呈显著相关(r2 = 0.95);0.05和磷(r2 = 0.99) (P <0.01)。
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引用次数: 0
Assessment of the Nutritional Content and Hedonic Test on Pameling Avocado from Three Different Altitudes 不同海拔地区Pameling牛油果的营养成分评价及欣赏性试验
Pub Date : 2023-01-01 DOI: 10.22161/ijeab.85.6
Paramyta Nila Permanasari, Anna Satyana Karyawati, Akbar Saitama, Karuniawan Puji Wicaksono
Malang Regency has superior avocado commodity which is so-called Pameling. Superiorities of the fruit are indicated by the large size of the fruit and quality of the fruit flesh which is liked by the consumers. Today, the development of Pameling avocado has expanded from the lowlands to the highlands in diverse regions. In order to obtain optimal harvest yields, both quantity and quality, the research affirmed the quality of Pameling avocado, which is grown at the lowlands and the highlands. The research was conducted by testing quality (nutritional content) and organoleptic. Results of the research showed that the avocados grown at the highlands were more preferred by the panelists based on the organoleptic test from the assessment aspects of appearance, texture, aroma, and taste. The lowlands produced preference of the color flesh which is mostly preferred by the panelists. Results of the nutritional test (quality) on fruits grown at the lowlands showed the highest level of sugar and protein. The middle plains are superior in fat content. Moreover, the highlands produce fruits with the highest coarse fibers. However, the differences in cultivation methods on three different altitudes will be the subject of further studies that need to be considered.
玛琅摄政有优质的牛油果商品,被称为Pameling。其优势在于果实体积大,果肉质量好,深受消费者喜爱。今天,帕美林鳄梨的发展已经从低地扩展到不同地区的高地。为了获得最优的收获产量,在数量和质量上,本研究对种植在低地和高地的Pameling牛油果的品质进行了肯定。通过质量(营养成分)和感官测试进行了研究。研究结果表明,通过感官测试,从外观、质地、香气和口感四个方面评估,高原鳄梨更受小组成员的青睐。低地产生了对肉色的偏好,这是小组成员最喜欢的。对生长在低地的水果进行营养(品质)测试的结果表明,其糖和蛋白质含量最高。中部平原地区的脂肪含量较高。此外,高地出产的水果含有最高的粗纤维。然而,三种不同海拔地区种植方法的差异将是进一步研究的主题,需要考虑。
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引用次数: 0
Analysis of the Potential Horticultural Products in Blitar Regency on the Agribusiness Market in Blitar Regency 英国农业综合经营市场潜力园艺产品分析
Pub Date : 2023-01-01 DOI: 10.22161/ijeab.85.7
Karuniawan Puji Wicaksono, Paramyta Nila Permanasari, Akbar Saitama
Blitar Regency has a wholesale market managed by the Wlingi Market Association, but some horticultural products from the farmers are still absorbed by the wholesale market outside Blitar Regency and reabsorbed by the retailers from Blitar Regency. Objectives of the research were to obtain information about the superior products, supply chain, and the inhibiting factors on the development of the horticultural products. Method of the research used descriptive analysis on each member of the links. Location of the research is the wholesale market in Blitar Regency, Wlingi. Variables of the research include types, volumes, and circulation of the horticultural products. Results of the research showed 3 major horticultural commodities that include chili, red pepper, and watermelon. The potential agricultural products in Blitar Regency have inefficient and complex supply chain because they involve many actors from farmers to consumers. The inhibiting factors are as follow: 1. The farmers do not have a collective system for marketing their crops so that information about the products as well as the prices are not integrated, 2. The supply chain patterns are inefficient and complex, so that the prices are costly, and 3. Inadequate location of the wholesale market. It is expected that the approach of supply chain on horticultural commodities in Blitar Regency will provide an overview of potential commodity supply availability as a consideration for supply chain management.
Blitar Regency有一个由Wlingi市场协会管理的批发市场,但是一些来自农民的园艺产品仍然被Blitar Regency外的批发市场吸收,并被Blitar Regency的零售商重新吸收。本研究旨在了解我国园艺产品发展的优势产品、供应链及制约因素。本研究采用描述性分析的方法对各成员的联系进行分析。研究地点是Wlingi Blitar Regency的批发市场。研究的变量包括园艺产品的类型、数量和流通。研究结果显示,3种主要的园艺商品包括辣椒、红辣椒和西瓜。由于涉及从农民到消费者的众多行为体,英国潜在农产品的供应链效率低下且复杂。抑制因素如下:1.抑制因素;农民没有一个集体的系统来销售他们的作物,所以关于产品和价格的信息没有整合。2 .供应链模式低效复杂,价格昂贵;批发市场位置不合理。预计在英国,园艺商品供应链的方法将提供潜在商品供应可用性的概述,作为供应链管理的考虑因素。
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引用次数: 0
Effect of Extract Euphorbia hirta Linn. Against Leaf and Fruit Pests on Plants Cucumber (Cucumis sativus Linn.) 大戟提取物的药理作用。黄瓜叶、果害虫防治
Pub Date : 2023-01-01 DOI: 10.22161/ijeab.82.18
Desi Pramida Sari, W. Warnita, Muhsanati Muhsanati
Soybean (Glycine max (L) Merril) is one of the agricultural commodities to help the food crop of the Indonesian people. Soybeans are one of the commodities that support the implementation of food crop diversification programs and support national food crop security. Increasing soybean productivity in ultisols can be done through liming using eggshell powder. This experiment has been implemented at Jalan Muhammad Hatta, RT 02, RW 08, Pauh, Padang, West Sumatera from October 2020 to March 2021. The purpose is to get the best eggshell powder dose to increase the growth and yield of the soybean on ultisol. The experiment method uses a complete random design (RAL) with 5 levels of eggshell powder treatment 0; 25; 50; 75; 100 g / plant. Data on the observation are analyzed statistically with the F test at a 5% level. If the F count of treatment is greater than the F table, proceed by the test DNMRT at α level of 5% level. The results showed the use of eggshell powder affected the growth and yield of soybean in ultisol to a dose is 25 g /plant.
大豆(Glycine max (L) merrill)是帮助印尼人民的粮食作物的农产品之一。大豆是支持实施粮食作物多样化计划和支持国家粮食作物安全的商品之一。利用蛋壳粉进行石灰化处理,可以提高多效土壤中大豆的产量。该实验已于2020年10月至2021年3月在西苏门答腊巴东保h的Jalan Muhammad Hatta, r02, rw08实施。目的是为了获得最佳的蛋壳粉用量,以促进大豆在多酚上的生长和产量。试验方法采用完全随机设计(RAL), 5个水平的蛋壳粉处理0;25;50;75;100克/株。观察数据在5%的水平上用F检验进行统计分析。如果处理的F计数大于F表,则继续在5%水平的α水平下进行DNMRT试验。结果表明,用25 g /株的多效土对大豆的生长和产量有影响。
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引用次数: 0
Microbial Assessment of Solid Waste and Bioaerosol Associated with Open Dumping Sites of the Kathmandu City, Nepal 与尼泊尔加德满都市露天倾倒场有关的固体废物和生物气溶胶的微生物评估
Pub Date : 2023-01-01 DOI: 10.22161/ijeab.83.7
Dibyak Kapali, Elisha Shakya, D. Pradhan, Elina Sigdel, Jyotin Shah, S. Thapaliya
The study aims to isolate and identify bacteria and fungi (mold) present in solid waste and its associated bioaerosol in the Kathmandu city. A total of 10 samples; 5 different solid waste samples and 5 different bioaerosol samples, collected from 5 open dumping sites in the Kathmandu city, were transported to the microbiology laboratory of St. Xavier’s College for processing. Standard microbiological procedures were followed for the identification of isolates. The Kirby-Bauer disk diffusion method was used to determine the antibiotic susceptibility of bacterial isolates following CLSI 2020 standards. In the collected solid waste samples, the bacterial colony count ranged from 1.27 × 108 to 2.8 × 108 CFU/ml, whereas the fungi colony count ranged from 1 × 105 to 4 × 105 CFU/ml. Bacterial colony counts from bioaerosol samples ranged from 116 to >300 CFU/90mm/15 minutes, whereas fungi colony counts were between 2 and 6 CFU/90mm/15 minutes. Out of 48 bacteria and 34 molds identified, Bacillus spp. (27%) and Aspergillus niger (29%) were found to be predominant than other isolates. Citrobacter spp., Salmonella spp., and Escherichia coli isolated from solid waste samples of dump site S3 showed maximum resistance to the different antibiotics used. The common microbial isolates from solid waste samples and bioaerosol samples included 7 different bacteria and 4 different molds. The presence of antibiotic-resistant bacteria and pathogenic fungi in waste dump sites pose public health-related risks.
该研究旨在分离和鉴定加德满都城市固体废物及其相关生物气溶胶中存在的细菌和真菌(霉菌)。共10个样本;从加德满都市5个露天垃圾场收集的5个不同固体废物样本和5个不同生物气溶胶样本被运送到圣泽维尔学院微生物实验室进行处理。按照标准微生物学程序鉴定分离物。采用Kirby-Bauer纸片扩散法对符合CLSI 2020标准的分离菌进行药敏试验。在所收集的固体废物样品中,细菌菌落计数范围为1.27 × 108 ~ 2.8 × 108 CFU/ml,真菌菌落计数范围为1 × 105 ~ 4 × 105 CFU/ml。生物气溶胶样品的细菌菌落计数范围为116至300 CFU/90mm/15分钟,而真菌菌落计数范围为2至6 CFU/90mm/15分钟。在鉴定的48种细菌和34种霉菌中,芽孢杆菌(27%)和黑曲霉(29%)占优势。从S3垃圾场固体废物样品中分离出的柠檬酸杆菌、沙门氏菌和大肠杆菌对不同抗生素的耐药性最大。固体废弃物样品和生物气溶胶样品中常见的微生物分离物包括7种不同的细菌和4种不同的霉菌。垃圾场中抗生素耐药细菌和致病真菌的存在构成公共卫生风险。
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International Journal of Environment, Agriculture and Biotechnology
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