International Journal of Health, Animal science and Food safety最新文献

{"title":"An exploratory study on the effects of rearing system and plumage colour on performance, carcass characteristics and meat quality of local turkeys","authors":"K. K. Safiyu, O. M. Sogunle, L. T. Egbeyale, T. Shittu","doi":"10.13130/2283-3927/10794","DOIUrl":"https://doi.org/10.13130/2283-3927/10794","url":null,"abstract":"In a bid to improve the productive potentials of local turkeys in developing countries, a total of 240 unsexed day-old poults arranged in a 2 × 2 factorial layout into 4 treatments with two rearing systems (indoor and outdoor) and two plumage colours (white and black) were used for this study. Poults were brooded for 4 weeks followed by an acclimatization period of 2 weeks in the two different rearing systems before the commencement of the study which lasted 10 weeks. Each treatment consisting of 60 birds was further sub-divided into six replicates of 10 birds per replicate. Data obtained were subjected to Analysis of Variance in a Completely Randomized Design. Results on performance in the grower phase showed turkeys reared in indoor system recorded significantly (p<0.05) higher weight gain (29.39 vs. 105.19 g/bird/day) and daily feed intake (27.18 vs. 98.11 g/bird/day) compare to turkeys under outdoor system. In addition, weight gain was significantly (p<0.05) higher (29.16 g/bird/day) in turkeys with black plumage than (27.42 g/bird/day) recorded in turkeys with white plumage. However, in the finisher phase turkeys under outdoor system recorded significantly (p<0.05) higher weight gain than turkeys under indoor system. In the finisher phase, interaction effects showed best weight gain and Feed Conversion Ratio (FCR) (39.22 g/bird/day and 4.60) in white-plumaged turkeys reared in outdoor system. Turkeys under outdoor system also had significantly (p<0.05) higher back and spleen percentages. However, proportions of thigh were significantly (p<0.05) higher in turkeys reared indoor. In addition, white-plumaged turkeys recorded significantly (p<0.05) higher (21.07%) cooking loss than 14.58% recorded in turkeys with black plumage. In conclusion, improved weight gain with best FCR at finisher phase as well as highest spleen portion and cooking loss in thigh meat was obtained in white-plumaged turkeys reared in outdoor system.","PeriodicalId":14105,"journal":{"name":"International Journal of Health, Animal science and Food safety","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73792004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Astragalus polysaccharide supplementation on growth performance and plasma parameters of weaned piglets under commercial condition","authors":"Xilong Li, Hui-Yuan Lü, Bangmin Liu, Xianren Jiang","doi":"10.13130/2283-3927/9949","DOIUrl":"https://doi.org/10.13130/2283-3927/9949","url":null,"abstract":"The aim of this study was to evaluate the effect of Astragalus polysaccharide (APS) supplementation on the growth performance, plasma biochemical parameters, and plasma immune and antioxidant indexes of weaned piglets in a commercial swine farm. A total of 120 piglets weaned at 22 days and allocated to 2 groups, and fed a basal diet either without (CTR) or with 200 mg/kg of APS in a local commercial farm for a 42-d experiment. At end of the trial, one piglet from each pen was selected for blood sampling. The results showed that dietary APS decreased the feed conversion ratio (FCR) compared to the CTR group from day 14 to day 28 and day 0 to day 42 (P = 0.08 and 0.02, respectively). In addition, supplementation of APS had the tendency to increase the plasma superoxide dismutase activity and IgG content of piglets compared to the CTR group on day 42 (P = 0.06 and 0.09, respectively). Results in this study suggested that dietary APS might have a beneficial effect on growth performance and health status of weaned piglets under the commercial condition.","PeriodicalId":14105,"journal":{"name":"International Journal of Health, Animal science and Food safety","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88864937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant-based strategies to control the zinc output from swine production","authors":"M. Hejna, A. Moscatelli, Nadia Stroppa, S. Piu, A. Baldi, L. Rossi","doi":"10.13130/2283-3927/10039","DOIUrl":"https://doi.org/10.13130/2283-3927/10039","url":null,"abstract":"Animal production systems produce large quantities of manure, which is recognized as a significant source of heavy metals (HMs) (Hejna et al., 2018). Some HMs are essential nutrients and zinc oxide (ZnO) was often used at high doses to control the enteric disorders mainly in the swine post weaning phase (Rossi et al ., 2013, 2014). The general increase of HMs content was registered in the livestock output with a negative impact on the environment. Preliminary data showed that swine manure was an important source of Zn, Cu, Mn and Se to the environment reflecting the HMs content in feeds (Hejna et al., 2017a). The aim of this study was to evaluate the ability of Typha latifolia (TL) and Thelypteris palustris (TP) to bioaccumulate trace elements, from water as a cost-effective plant-based approach of wastewater remediation in pig livestock. The experimental design included four mesocosms (width: 4.0 m, length: 2.0 m, depth: 0.7 m; 695L of water, 210kg of soil): two controls, planted with TP (TPC) and TL (TLC) respectively and two treated, planted with TP (TPT) and TL (TLT) respectively. The treatment was represented by a mineral feed additive premix dissolved in TPT and TLT with the following final HMs concentration: Zn: 44.02mg/L; Cu: 8.63mg/L; Mn: 10.83mg/L; Se: 0.09mg/L. Such high concentrations, corresponding to polluted wastewater, would be sufficient to reach the potential saturation limit of the substrates in the short experimental period. At day 0 (T0), day 15 (T1) and day 45 (T2) samples of roots, leaves, stems, soil and water were collected, dried and principal chemical component were estimated according to the official method of Analysis of Association of Analytical Communities (AOAC). Samples were also mineralized by an ultravawe single reaction chamber and analyzed using inductively coupled plasma mass spectrometry (ICP-MS). Obtained results showed that TL and TP tolerated high levels of Zn, Cu, Mn and Se with no visual toxicity signs or significant effects on growth during the entire experimental period. TP appeared more effective than TL at translocating elements from water to plant tissues. In particular, TPT showed a significant increase of Zn and Cu content in whole plants from T0 to T2 (p≤0.001). At T2 the mean zinc concentration was 409.26±342.33 mg/kg d.m. and 271.64±64.85 mg/kg d.m. in TPT and TLT plants respectively. In the plants of control mesocosms, differences between T0 and T2 were not observed. Results suggested that both plant species were able to reduce the available amount of metals from the contaminated wastewater, thus TL and TP plants may be candidates for the phytoremediation approach to control HMs output from the livestock wastewater. Acknowledgements: supported by MIPAAF 2015.","PeriodicalId":14105,"journal":{"name":"International Journal of Health, Animal science and Food safety","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73941714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical effects of dexmedetomidine combined with methadone after intranasal and intramuscular administration in dogs","authors":"D. Gioeni, F. D. Cesare, E. D'urso, V. Rabbogliatti, G. Ravasio","doi":"10.13130/2283-3927/10042","DOIUrl":"https://doi.org/10.13130/2283-3927/10042","url":null,"abstract":"The intranasal (IN) route shows promise for chemical restraint given the large area offered for drugs absorption. The nasal turbinates increase nasal mucosa surface, which have a greater blood flow than muscle, brain and liver tissue . Aim of the study is to compare the clinical effects and sedation scores following either IN or intramuscular (IM) administration of dexmedetomidine-methadone in dogs. Twenty mixed-breed, client-owned, healthy dogs, undergoing soft tissue surgery or diagnostic procedures, were randomly allocated in two groups (n = 10) to receive dexmedetomidine (0.01 mg kg-1) together with methadone (0.4 mg kg-1) IN (IN-group) or IM (IM-group). Temperament was evaluated before premedication (1 = calm and friendly, 4 = very excitable or nervous) (Maddern et al. 2010). Heart rate (HR), respiratory frequency (fR), body temperature, and side effects were recorded before (T0) and 10 (T10), 20 (T20) and 30 (T30) minutes after premedication. Sedation was scored 3 times (every 10 minutes) after drugs administration using a descriptive sedation scale (0 = no sedation, 13 = extremely sedated). Induction was performed at T30 with titrate-to-effect propofol and the dosage was recorded. Student T-test was performed. Weight, age, temperament, body temperature and propofol dose were not different between groups. At each time point, excluding T0, IM-group showed a statistically lower HR and fRcompared to IN-group. No undesirable effects were observed in both groups. Sedation score in IM-group was significantlyhigher compared to IN-group at each time point. In conclusion, despite statistical differences, IN administration produces a satisfactory clinical sedation with more gradual hemodynamic effects compared to IM injection; this is probably due to a direct transport of drugs from cranial nerves (I-V) to brain with limited systemic absorption. However, the high variability recorded in sedation score between subjects in IN-group (min 1/13; max 13/13 at T30) probably arises from a variable drugs conveyance from nasal mucosae to target cell in CNS by IN administration.","PeriodicalId":14105,"journal":{"name":"International Journal of Health, Animal science and Food safety","volume":"253 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77531643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Planar Lymphoscintigraphy for sentinel lymph node mapping in dogs with mast cell tumor: a pilot study.","authors":"M. Manfredi, D. Zani, Davide Danilo Zani","doi":"10.13130/2283-3927/10022","DOIUrl":"https://doi.org/10.13130/2283-3927/10022","url":null,"abstract":"The histopathological assessment of the first node receiving lymphatic drainage from a tumor – defined as Sentinel Lymph Node (SLN) – is essential to determine stage, therapy and outcome in oncological patients. Both in human and veterinary medicine, lymphoscintigraphy is a recognized procedure for SLN detection (Mariani et al. , 2004; Tuohy et al ., 2009; Beer et al ., 2017). In this study, we want to determine the most suitable pre-operative planar lymphoscintigraphy protocol for SLN mapping in dogs with mast cell tumor (MCT). We selected 5 dogs diagnosed with cutaneous MTC, with clinically negative lymph nodes and no distant metastasis, undergoing surgical tumor removal, and we obtained owner’s written consent. Planar lymphoscintigraphy was performed in patients under general anesthesia, after subcutaneous peritumoral injection of different doses of technetium-99m (Tc-99m) labelled colloid diluted reaching a 0.5 ml volume (Worley, 2014). The MegaBecquerel value (MBq) of the syringe was measured before and after the injection. Dynamic images (1 frame/second for 60 seconds) were taken at the moment of the injection, 3 and 8 minutes after the injection. Ventrodorsal (VD) and lateral (L) static images (120 seconds/frame) were taken until the identification of SLN had been made. If needed, the injection site was masked with a 2-mm lead foil. Results are showed in Table 1. In patient 1, the SLN was not identify, probably due to a superimposition with the injection site. During the study, we increased the injected MBq dose, in order to better visualize lymphatic path and SLN (Balogh et al. , 2002). In fact, the number of static images needed to identify SLN have been reduced from 8,7 to 6. Masking the injection site proved to be useful for a better visualization of SLN. Dynamic images showed to be unnecessary for the SLN identification. For further studies, we suggest the injection of minimum 23,5 MBq Tc-99m activity and the acquisition of VD and L static images with and without masking the injection site.","PeriodicalId":14105,"journal":{"name":"International Journal of Health, Animal science and Food safety","volume":"138 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86822635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary evaluation results of the extraction methods for Fipronil and its metabolites and Amitraz in chicken eggs","authors":"Shih-kuo Lin, L. Chiesa, E. Pasquale","doi":"10.13130/2283-3927/10015","DOIUrl":"https://doi.org/10.13130/2283-3927/10015","url":null,"abstract":"Fipronil and Amitraz are broadly used insecticides for the treatment or prevention for animal health, indoor pest control, and commercial crop protection. As the use of Fipronil or Amitraz on food-producing animals was not allowed by the EU legislation, the Maximum Residue Limit (MRL) values of Fipronil and Amitraz were set at the detection limit of 5 ng mL-1 and 10 ng mL-1, respectively. According to the database of Rapid alert system for food and feed (RASFF), after the Belgian authority reported Fipronil residues in chicken eggs in 2017, there were 719 follow-up reports from 34 countries. Fipronil and Amitraz are included in the Italian National Residue Program so it is necessary to develop a selective, sensitive, specific and rapid method. Three extraction methods were evaluated on fresh egg blank samples to determine the presence of Fipronil, as well as its metabolites and Amitraz. In the solvent-salt method the sample was added by water, NaCl and formic acetonitrile, followed by hexane to remove potential fat. In the Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) method the sample was extracted by Superl® Que Citrate powder and acetonitrile, followed by Superl® PSA powder. In the water-associated QuEChERS method the sample was mixed with water and acetonitrile, followed by Superl® Que Citrate powder, then the supernatant was collected and mixed with CaCl2. The analyses of the extracts were performed with high performance liquid chromatography coupled to Q-Exactive Orbitrap high-resolution mass spectrometer (LC-HRMS). Furthermore, Thompson (2000) mentioned that the Coefficient of variation (CV) is acceptable if it is lower than 22%. Based on the obtained recovery values (72 to 113%) and CV (1.67 to 14.69%), the water-associated QuEChERS method was selected because the recoveries rates obtained with the other methods were lower than 70%.Calibration curves exhibited correlation values ranging from 0.9653 to 0.9999(Figure 1); the limits of detection ranged from 0.08 to 1.21 ng mL-1, and the limits of quantification were from 0.28 to 4.04 ng mL-1. The preliminary results fulfilled the European criteria for the validation of the analytical methods. Further analyses have been performed to evaluate the repeatability and reproducibility.","PeriodicalId":14105,"journal":{"name":"International Journal of Health, Animal science and Food safety","volume":"161 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83163933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Helical Hydro-Computed Tomography in the diagnosis of pyloric stenosis in two foals","authors":"V. Rabbogliatti, M. Giancamillo, D. Zani, D. Gioeni, F. D. Cesare, E. D'urso, G. Ravasio, Davide Danilo Zani","doi":"10.13130/2283-3927/10048","DOIUrl":"https://doi.org/10.13130/2283-3927/10048","url":null,"abstract":"Pyloric stenosis is rarely reported in horses (Bart et al 1980; Bezdecova et al. 2009). Congenital and acquired conditions have been described (Church et al 1986; Heidmann et al 2004; Laing et al. 1992; McGill et al. 1984) clinical suspicion is based on clinical findings while definitive diagnosis is reached by exploratory laparotomy and gastroscopy. The use of other diagnostic techniques has never been described. Two foals were admitted for recurrent abdominal pain. Clinical and ultrasonographic (US) examinations were performed.  Foals underwent Computed Tomography (CT) of the abdomen, both native and Helical Hydro-CT. US revealed mild stomach distension, mild small bowel wall thickening; small intestine obstruction was suspected in both foals. Case 1, two-month old Thoroughbred filly 130 kg of weight: CT showed segmental concentric pylorus stenosis, at the pyloric duodenal junction level. Mild liquid distension of the pyloric antrum and mixed gas and fluid distension of the cranial duodenum. During necroscopy the pyloric antrum showed stenosis due to an inelastic constricting ring reducing the lumen of the pyloric canal. The glandular part presented mild acute catarrhal gastritis. Case 2, three-month old Italian Saddle colt 142 kg of weigh: CT showed small amount of intraluminal hyperattenuanting material within the gastric fundus and duodenum. Hydro-CT highlighted the presence of mild pylorus narrowing, mild distension and moderate mucosal irregularities of the pyloric antrum. An acquired pyloric stenosis secondary to chronic gastritis of unknown origin was suspected. Explorative laparotomy was performed; the antrum was mildly distended and the pylorus appeared narrowed and hard on palpation; gastrojejunostomy was performed. Ante-mortem diagnosis of pyloric stenosis in horses is challenging because aspecific clinical signs. Native CT allowed to investigate both the stomach and the small intestine and, in one case, outlined the presence of pylorus stenosis. In case 2, only Helical Hydro-CT allowed better evaluation of the pyloric thickness. CT and Helical Hydro-CT can be considered a useful diagnostic tool in foal with clinical suspicion of pyloric stenosis.","PeriodicalId":14105,"journal":{"name":"International Journal of Health, Animal science and Food safety","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85301176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of a novel bacterium of the genus Midichloria (family Midichloriaceae) in avian-borne Hyalomma marginatum ticks and their trans-Saharan migratory hosts","authors":"V. Serra, C. Bazzocchi, Irene Di Lecce","doi":"10.13130/2283-3927/10006","DOIUrl":"https://doi.org/10.13130/2283-3927/10006","url":null,"abstract":"Introduction: Ticks are haematophagous ectoparasites of vertebrates habitually parasitizing avian species, which may contribute to tick dispersal across continents during migrations (Hasle 2013; Altizer et al., 2011). Midichloria bacteria can be transmitted to the vertebrate host during the tick bite (Bazzocchi et al., 2013; Serra et al., 2018). Although many avian species are common hosts of ticks harbouring Midichloria (e.g. Ixodes, Hyalomma ), the circulation of this bacterium in birds has never been investigated. The aims of this study are: 1) evaluate the presence of Midichloria DNA in H. marginatum ticks and blood collected from trans-Saharan migratory birds; 2) quantify Midichloria bacteria in ticks through a novel quantitative PCR (qPCR). Material and methods: A total of 256 H. marginatum ticks and 97 blood samples were collected from three different migratory species ( Phoenicurus phoenicurus, Saxicola rubetra and Sylvia communis ) on Ventotene Island (Central Italy) and DNAs were extracted. A nested-PCR targeting the 16S rRNA gene of Midichloria was used to detect bacterial presence. Subsequently, primers targeting the gyrB gene of Midichloria and the cal gene of H. marginatum were designed and used in a qPCR for Midichloria quantification. Results were expressed as gyrB / cal copy numbers ratio. Results and discussion: 94% of Hyalomma ticks harbored DNA of Midichloria belonging to the monophylum associated with ticks, while the bacterial DNA was detected in 44.3% of blood samples. Furthermore, engorged ticks showed significantly higher bacteria load than unengorged ticks (Table 1; Wilcoxon sum-rank test: z=3.14; p=0.0017), similarly to what has been observed for M. mitochondrii in I. ricinus ticks. Conclusions: This work provides evidence for the presence of circulating Midichloria DNA in long-distance migratory birds, suggesting an enhanced worldwide spread of these bacteria across haematophagous ectoparasite populations. Future studies are necessary to increase the knowledge of Midichloria role in the biology of this tick species.","PeriodicalId":14105,"journal":{"name":"International Journal of Health, Animal science and Food safety","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91000788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Locoregional anaesthesia for perioperative pain management in rabbits undergoing Total Ear Canal Ablation and Lateral Bulla Osteotomy (TECALBO): case series.","authors":"E. D'urso, D. Zani, Massimo Dacerno, D. Gioeni, V. Rabbogliatti, G. Ravasio","doi":"10.13130/2283-3927/10046","DOIUrl":"https://doi.org/10.13130/2283-3927/10046","url":null,"abstract":"TECALBO is an effective yet invasive surgical technique to treat end-stage internal and external otitis in rabbits. The aim of this study is to describe a new locoregional analgesic approach, as until now only systemic analgesia was described for this surgery in rabbits. The first patient, a 7-year-old lop-eared neutered male weighing 2.1 kg, undergoing right TECALBO due to end-stage otitis with bulla empyema, received subcutaneous dexmedetomidine (60 µg/kg) and ketamine (10 mg/kg). Propofol 0.5% (1 mg/kg) was administered for induction while titrate-to-effect isoflurane was administered for maintenance. After palpating the zygomatic arch, the mandibular nerve was blocked at the level of the temporomandibular joint. The auriculopalpebral nerve was blocked in the centre of a triangle identified between the base of the pinna and the occipital crest. Ropivacaine 0.5% (1.5 mg/kg per block) was injected after neurolocation of the nerves. Neither intraoperative (mean heart and respiratory rate 175 ± 22 and 25 ± 5 respectively) nor postoperative nociception were shown as no rescue analgesia was needed. Full recovery and food intake occurred within 1 hour after awakening. The second patient, a 9-year-old Dutch-belted intact female of 1.5 kg undergoing right TECALBO for end-stage otitis, received dexmedetomidine (40 µg/kg), ketamine (7 mg/kg) and midazolam (0.3 mg/kg) subcutaneously. Propofol 0.5% (2 mg/kg) was administered for induction. Titrate-to-effect isoflurane was administered for maintenance. Mandibular and auriculopalpebral nerve blocks were performed as described. No intraoperative (mean heart and respiratory rate 195 ± 15 and 31 ± 7 respectively) nor postoperative nociception were recorded, despite delayed awakening and full recovery were shown (food intake occurred 2.5 hours after awakening) and were probably related to midazolam administration. In both cases, the combination of mandibular and auriculopalpebral blocks appeared to provide effective perioperative analgesia for TECALBO surgery in rabbits.","PeriodicalId":14105,"journal":{"name":"International Journal of Health, Animal science and Food safety","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91010269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Follicle-like environment for domestic cat vitrified oocytes.","authors":"M. Colombo, M. Morselli, G. Luvoni","doi":"10.13130/2283-3927/10005","DOIUrl":"https://doi.org/10.13130/2283-3927/10005","url":null,"abstract":"The in vitro development of vitrified oocytes (VOs) is still suboptimal (Mandawala et al., 2016) and the traditional two-dimensional (2D) culture systems might not be adequate to fully exploit VOs potential. The use of three-dimensional (3D) follicle-like structures, i.e. a combination of granulosa cells (GCs) and semipermeable 3D matrices, could mimic the physiological microenvironment and enhance VOs maturation and embryo development.The aim of this study was to assess the steroidogenic ability (estradiol and progesterone secretion) of GCs encapsulated in 3D barium alginate microcapsules (follicle-like structure) compared to GCs cultured in a 2D monolayer and the maturation outcomes of VOs cultured in these systems.After purification (Simsek & Arikan, 2015), cat GCs retrieved from isolated ovaries were in vitro cultured for 6 days in 3D microcapsules (Vigo et al., 2005) or in 2D monolayers. On days 2 and 6, conditioned medium was collected and hormonal determination by enzyme-linked fluorescent assay was performed. On the same days, 3D and 2D cultured GCs were used as artificial milieu for in vitro maturation of VOs obtained by Cryotop protocol. Nuclear maturation was assessed by bis-benzimide staining.Steroidogenesis was observed in 3D follicle-like structures as well as in 2D monolayers; hormonal concentration increased over time and on day 6 it significantly differed between systems (p=0.02). Vitrified oocytes resumed meiosis in presence of GCs cultured for 2 days (3D: 45.5%; 2D: 56.7%), while GCs cultured for 6 days significantly hindered VOs meiosis progression in monolayers (21.7%, p=0.007), but supported high proportions of full maturation in follicle-like structures (26.7%, p=0.07).Granulosa cells in 3D microcapsules maintained their physiological features and these follicle-like structures were able to restore VOs developmental abilities. However, further advancements in VOs culture methods would optimize the use of these valuable resources.","PeriodicalId":14105,"journal":{"name":"International Journal of Health, Animal science and Food safety","volume":"26 3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86073355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}