Pub Date : 2020-03-30DOI: 10.11620/ijob.2020.45.1.8
Kyung-In Jeong, Su-Gwan Kim, Dae-San Go, Do Kyung Kim
Bilobalide isolated from the leaves of Ginkgo biloba has several pharmacological activities such as neuroprotective, anti-inflammatory, and anticonvulsant. However, the effect of bilobalide on cancer has not been clearly established. The main purpose of this study was to investigate the effect of bilobalide on cell growth and apoptosis induction in FaDu human pharyngeal squamous cell carcinoma. This was examined by 3-[4,5-dimethylthiazol-2-yl]-2,5- diphenyltetrazolium bromide assay, nuclear 4′,6-diamidino-2-phenylindole dihydrochloride staining, DNA fragmentation analysis, and immunoblotting. Bilobalide inhibited the growth of FaDu cells in dose- and time-dependent manners. Treatment with bilobalide resulted in nuclear condensation and DNA fragmentation in FaDu cells. Furthermore, it promoted the proteolytic cleavage of procaspase-3/-7/-8/-9 with increase in the amount of cleaved caspase-3/-7/-8/-9. Bilobalide-induced apoptosis in FaDu cells was mediated by the expression of Fas and the activation of caspase-8, caspase-3, and poly (ADP-ribose) polymerase. Immunoblotting revealed that the antiapoptotic mitochondrial protein Bcl-2 was downregulated, but the proapoptotic protein Bax was upregulated by bilobalide in FaDu cells. Bilobalide significantly increased Bax/Bcl-2 ratio. These results suggest that bilobalide inhibits cell proliferation and induces apoptosis in FaDu human pharyngeal squamous cell carcinoma via both the death receptor-mediated extrinsic apoptotic pathway and the mitochondrial-mediated intrinsic apoptotic pathway.
{"title":"Inhibition of cell growth and induction of apoptosis by bilobalide in FaDu human pharyngeal squamous cell carcinoma","authors":"Kyung-In Jeong, Su-Gwan Kim, Dae-San Go, Do Kyung Kim","doi":"10.11620/ijob.2020.45.1.8","DOIUrl":"https://doi.org/10.11620/ijob.2020.45.1.8","url":null,"abstract":"Bilobalide isolated from the leaves of Ginkgo biloba has several pharmacological activities such as neuroprotective, anti-inflammatory, and anticonvulsant. However, the effect of bilobalide on cancer has not been clearly established. The main purpose of this study was to investigate the effect of bilobalide on cell growth and apoptosis induction in FaDu human pharyngeal squamous cell carcinoma. This was examined by 3-[4,5-dimethylthiazol-2-yl]-2,5- diphenyltetrazolium bromide assay, nuclear 4′,6-diamidino-2-phenylindole dihydrochloride staining, DNA fragmentation analysis, and immunoblotting. Bilobalide inhibited the growth of FaDu cells in dose- and time-dependent manners. Treatment with bilobalide resulted in nuclear condensation and DNA fragmentation in FaDu cells. Furthermore, it promoted the proteolytic cleavage of procaspase-3/-7/-8/-9 with increase in the amount of cleaved caspase-3/-7/-8/-9. Bilobalide-induced apoptosis in FaDu cells was mediated by the expression of Fas and the activation of caspase-8, caspase-3, and poly (ADP-ribose) polymerase. Immunoblotting revealed that the antiapoptotic mitochondrial protein Bcl-2 was downregulated, but the proapoptotic protein Bax was upregulated by bilobalide in FaDu cells. Bilobalide significantly increased Bax/Bcl-2 ratio. These results suggest that bilobalide inhibits cell proliferation and induces apoptosis in FaDu human pharyngeal squamous cell carcinoma via both the death receptor-mediated extrinsic apoptotic pathway and the mitochondrial-mediated intrinsic apoptotic pathway.","PeriodicalId":14180,"journal":{"name":"International Journal of Oral Biology","volume":"10 1","pages":"8-14"},"PeriodicalIF":0.0,"publicationDate":"2020-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86542455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-31DOI: 10.11620/ijob.2019.44.4.173
Eun‐Ji Oh, Y. Zang, Jung-Woo Kim, M. N. Lee, J. Song, Sin‐Hye Oh, Seung-Hee Kwon, Jin-Woo Yang, J. Koh
{"title":"CCR5 deficiency in aged mice causes a decrease in bone mass","authors":"Eun‐Ji Oh, Y. Zang, Jung-Woo Kim, M. N. Lee, J. Song, Sin‐Hye Oh, Seung-Hee Kwon, Jin-Woo Yang, J. Koh","doi":"10.11620/ijob.2019.44.4.173","DOIUrl":"https://doi.org/10.11620/ijob.2019.44.4.173","url":null,"abstract":"","PeriodicalId":14180,"journal":{"name":"International Journal of Oral Biology","volume":"50 1","pages":"173-181"},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75940495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-31DOI: 10.11620/ijob.2019.44.4.160
So‐Hee Kim, I. Kang
{"title":"Comparison of inflammatory cytokine-inducing activity of lipopolysaccharides from major periodontal bacteria","authors":"So‐Hee Kim, I. Kang","doi":"10.11620/ijob.2019.44.4.160","DOIUrl":"https://doi.org/10.11620/ijob.2019.44.4.160","url":null,"abstract":"","PeriodicalId":14180,"journal":{"name":"International Journal of Oral Biology","volume":"104 1","pages":"160-164"},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89839877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-31DOI: 10.11620/ijob.2019.44.4.191
Lim Yun-Kyong, Yoon, So-Young, Lee Dae-Sung, Kook Joong-Ki
{"title":"Antimicrobial effect of Australia propolis on cariogenic and periodontopathic bacteria","authors":"Lim Yun-Kyong, Yoon, So-Young, Lee Dae-Sung, Kook Joong-Ki","doi":"10.11620/ijob.2019.44.4.191","DOIUrl":"https://doi.org/10.11620/ijob.2019.44.4.191","url":null,"abstract":"","PeriodicalId":14180,"journal":{"name":"International Journal of Oral Biology","volume":"343 1","pages":"191-194"},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79579484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-31DOI: 10.11620/ijob.2019.44.4.125
K. Baek
{"title":"An overview of the endocrine functions of osteocalcin","authors":"K. Baek","doi":"10.11620/ijob.2019.44.4.125","DOIUrl":"https://doi.org/10.11620/ijob.2019.44.4.125","url":null,"abstract":"","PeriodicalId":14180,"journal":{"name":"International Journal of Oral Biology","volume":"15 1","pages":"125-129"},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85807818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-31DOI: 10.11620/ijob.2019.44.4.182
Gyuseok Lee, Won-Hyun Song, Su-jin Kim, Young-Gwon Kim, J. Ryu
{"title":"Apelin-APJ axis inhibits TNF-alpha-mediated expression of genes involved in the inflammatory response in periodontal ligament cells","authors":"Gyuseok Lee, Won-Hyun Song, Su-jin Kim, Young-Gwon Kim, J. Ryu","doi":"10.11620/ijob.2019.44.4.182","DOIUrl":"https://doi.org/10.11620/ijob.2019.44.4.182","url":null,"abstract":"","PeriodicalId":14180,"journal":{"name":"International Journal of Oral Biology","volume":"39 1","pages":"182-190"},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84536143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-31DOI: 10.11620/ijob.2019.44.4.130
S. Ko
{"title":"Effects of plant-derived natural products on inflammatory bone destructive disease","authors":"S. Ko","doi":"10.11620/ijob.2019.44.4.130","DOIUrl":"https://doi.org/10.11620/ijob.2019.44.4.130","url":null,"abstract":"","PeriodicalId":14180,"journal":{"name":"International Journal of Oral Biology","volume":"84 1","pages":"130-143"},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76176418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-31DOI: 10.11620/ijob.2019.44.4.165
Younho Han, W. Park
{"title":"Discovery and characterization of berberine derivatives as stimulators of osteoblast differentiation","authors":"Younho Han, W. Park","doi":"10.11620/ijob.2019.44.4.165","DOIUrl":"https://doi.org/10.11620/ijob.2019.44.4.165","url":null,"abstract":"","PeriodicalId":14180,"journal":{"name":"International Journal of Oral Biology","volume":"5 1","pages":"165-172"},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89745176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-31DOI: 10.11620/ijob.2019.44.4.144
Jaehyun Park, Cellular Reprogramming, Sangkyu Park, Jeongmin Seo, S. Roh
{"title":"Effect of the hedgehog signaling pathway on hair formation-related cells","authors":"Jaehyun Park, Cellular Reprogramming, Sangkyu Park, Jeongmin Seo, S. Roh","doi":"10.11620/ijob.2019.44.4.144","DOIUrl":"https://doi.org/10.11620/ijob.2019.44.4.144","url":null,"abstract":"","PeriodicalId":14180,"journal":{"name":"International Journal of Oral Biology","volume":"19 1","pages":"144-151"},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80087765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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