Diabetes mellitus is becoming a common metabolic disorder which has serious threat to public health in the world. There are chemicals and biochemical agent that helps in controlling diabetes but there is no permanent remedy available which helps to get recovered completely from this disorder. By conducting large number of research work, numerous traditional medicines have been found for diabetes. Substances and extracts isolated from different natural resources especially plants have always been a rich arsenal for controlling and treating diabetes problem and complication arising due to it. So this review helps the reader to understand the importance of various types of herbal and polyherbal formulations present traditionally which can be used to treat diabetes mellitus.
{"title":"Diabetes mellitus treatment using herbal drugs","authors":"S. Verma, Madhu Gupta, H. Popli, G. Aggarwal","doi":"10.5138/09750185.2181","DOIUrl":"https://doi.org/10.5138/09750185.2181","url":null,"abstract":"Diabetes mellitus is becoming a common metabolic disorder which has serious threat to public health in the world. There are chemicals and biochemical agent that helps in controlling diabetes but there is no permanent remedy available which helps to get recovered completely from this disorder. By conducting large number of research work, numerous traditional medicines have been found for diabetes. Substances and extracts isolated from different natural resources especially plants have always been a rich arsenal for controlling and treating diabetes problem and complication arising due to it. So this review helps the reader to understand the importance of various types of herbal and polyherbal formulations present traditionally which can be used to treat diabetes mellitus.","PeriodicalId":14199,"journal":{"name":"International Journal of Phytomedicine","volume":"4 1","pages":"01-10"},"PeriodicalIF":0.0,"publicationDate":"2018-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82051884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Saad Mebrek, H. Djeghim, Y. Mehdi, Asma Meghezzi, Sirajudheen Anwar, N. Awadh, M. Benali
Beta-glucan, such as barley-derived beta-glucan (BBG), are homopolysaccharides that have attracted attention by their nutritional and therapeutic properties. The aim of this study was to evaluate the antioxidant power of BBG extracted from local Algerian variety of barley (SAIDA 183), and its acetylcholinesterase, alpha glucosidase inhibitory activity as well as its prebiotic potential by fermentation with lactic acid bacteria isolated from camel’s milk, namely lactococcuslactisssplactis (Lc.l.l) and leuconostocmesenteroidesspmesenteroides (Ln.m.m). The results revealed that BBG exhibited low activity against DPPH and ferric-reducing power (IC 50 4018.61 ± 656.69 and A 0.5 at 359.88 ±63.64 µg/mL respectively), in contrast to other antioxidant tests (ABTS, Beta-carotene and CUPRAC) where BBG demonstrated a moderate activity (IC 50 529.91 ±26.37, IC 50 161.013±13.322, A 0.5 529.79 ± 48.65 µg/mL). The scavenging ability of hydroxyl radical and superoxide radical by BBG with an IC 50 at 2268.38±101.57 µg/mL and IC 50 345.26± 62.32 µg/mL, respectively, while enzymatic inhibition by BBG exhibited for AChE at IC 50 859.164 ±64.46 μg/mL , BChE at IC 50 at 725.470 ±30.95 , α-Amylase inhibitory activity at IC 50 2986.785 ± 37.046 . The bacterial growth of the two strains used in this study is favorably affected by the use of BBG as the only carbon source, in comparison with glucose as a control. In light of these findings, it can be concluded that BBG have shown moderate antioxidant and enzyme inhibitory activities and can be used as a prebiotic by acting synergistically with probiotics in functional food matrices.
{"title":"Antioxidant, anti-cholinesterase, anti-α-glucosidase and prebiotic properties of beta-glucan extracted from Algerian barley","authors":"Saad Mebrek, H. Djeghim, Y. Mehdi, Asma Meghezzi, Sirajudheen Anwar, N. Awadh, M. Benali","doi":"10.5138/09750185.2229","DOIUrl":"https://doi.org/10.5138/09750185.2229","url":null,"abstract":"Beta-glucan, such as barley-derived beta-glucan (BBG), are homopolysaccharides that have attracted attention by their nutritional and therapeutic properties. The aim of this study was to evaluate the antioxidant power of BBG extracted from local Algerian variety of barley (SAIDA 183), and its acetylcholinesterase, alpha glucosidase inhibitory activity as well as its prebiotic potential by fermentation with lactic acid bacteria isolated from camel’s milk, namely lactococcuslactisssplactis (Lc.l.l) and leuconostocmesenteroidesspmesenteroides (Ln.m.m). The results revealed that BBG exhibited low activity against DPPH and ferric-reducing power (IC 50 4018.61 ± 656.69 and A 0.5 at 359.88 ±63.64 µg/mL respectively), in contrast to other antioxidant tests (ABTS, Beta-carotene and CUPRAC) where BBG demonstrated a moderate activity (IC 50 529.91 ±26.37, IC 50 161.013±13.322, A 0.5 529.79 ± 48.65 µg/mL). The scavenging ability of hydroxyl radical and superoxide radical by BBG with an IC 50 at 2268.38±101.57 µg/mL and IC 50 345.26± 62.32 µg/mL, respectively, while enzymatic inhibition by BBG exhibited for AChE at IC 50 859.164 ±64.46 μg/mL , BChE at IC 50 at 725.470 ±30.95 , α-Amylase inhibitory activity at IC 50 2986.785 ± 37.046 . The bacterial growth of the two strains used in this study is favorably affected by the use of BBG as the only carbon source, in comparison with glucose as a control. In light of these findings, it can be concluded that BBG have shown moderate antioxidant and enzyme inhibitory activities and can be used as a prebiotic by acting synergistically with probiotics in functional food matrices.","PeriodicalId":14199,"journal":{"name":"International Journal of Phytomedicine","volume":"10 1","pages":"58-67"},"PeriodicalIF":0.0,"publicationDate":"2018-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76239703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bryonopsis laciniosa also known as “Shivlingi” annual climber with bright red fruits and is reported to be highly medicinal in India . As a folk medicine, the plant is used in treatment of broad range of diseases and disorders. In the present study, Hexane extract of B. laciniosa fruits were used to evaluate in vitro anti inflammatory, antioxidant and Cytotoxicity (towards MCF-7 cell line) activities. In vitro anti inflammatory activity by inhibition of protein denaturation, antioxidant assays like DPPH, ABTS, H 2 O 2 and FRAP were used to measure the antioxidant capacity of the hexane extracts and cytotoxicity activity using MCF-7 breast cancer cell line. Hexane extract showed the effective antioxidant activity in all assays compared to ascorbic acid and BHT. The results for In vitro anti inflammatory activity of hexane extract and Dichlofenac drug were equivalent, hexane extract showed promising activity for inhibition of protein denaturation assay. The cytotoxicity activity from hexane extract was noticeable against MCF-7 cell line. The overall results show potential application of Bryonopsis laciniosa fruits suggesting their potential application as a health-promoting functional ingredient or natural preservative in foods.
{"title":"In vitro antioxidant Anti-inflammatory and Cytotoxicity Activities from Hexane Extract of Bryonopsis laciniosa Fruits","authors":"S. C.B., R. Londonkar, Umesh Madire Kattegouda","doi":"10.5138/09750185.1835","DOIUrl":"https://doi.org/10.5138/09750185.1835","url":null,"abstract":"Bryonopsis laciniosa also known as “Shivlingi” annual climber with bright red fruits and is reported to be highly medicinal in India . As a folk medicine, the plant is used in treatment of broad range of diseases and disorders. In the present study, Hexane extract of B. laciniosa fruits were used to evaluate in vitro anti inflammatory, antioxidant and Cytotoxicity (towards MCF-7 cell line) activities. In vitro anti inflammatory activity by inhibition of protein denaturation, antioxidant assays like DPPH, ABTS, H 2 O 2 and FRAP were used to measure the antioxidant capacity of the hexane extracts and cytotoxicity activity using MCF-7 breast cancer cell line. Hexane extract showed the effective antioxidant activity in all assays compared to ascorbic acid and BHT. The results for In vitro anti inflammatory activity of hexane extract and Dichlofenac drug were equivalent, hexane extract showed promising activity for inhibition of protein denaturation assay. The cytotoxicity activity from hexane extract was noticeable against MCF-7 cell line. The overall results show potential application of Bryonopsis laciniosa fruits suggesting their potential application as a health-promoting functional ingredient or natural preservative in foods.","PeriodicalId":14199,"journal":{"name":"International Journal of Phytomedicine","volume":"111 1","pages":"23-29"},"PeriodicalIF":0.0,"publicationDate":"2018-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79628284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Polyherbal medicines are widely used for the treatment of various diseases in the developing countries. In order to validate their ability in boosting the immune system of tuberculosis patients, the mineral nutrients and vitamins present were determined. Their nutritive properties were analysed using an inductively coupled plasma optical emission spectrometer, while the vitamins were determined using standardized methods. The polyherbal preparations were found to be rich in mineral nutrients and vitamins. Calcium was the highest mineral nutrient detected, while the lowest nutrient was phosphorus. Quantitatively, calcium and magnesium contents in the remedies ranged from 973.30 to 6503.30 mg/100g and 80.00 to 406.00 mg/100g respectively. The amount of phosphorus and potassium was between 20.00 and 263.30 mg/100g; 160.00 and 2050.00 mg/100g respectively. Micro nutrients such as iron, manganese, zinc, aluminium and copper were also detected. Iron was the highest nutrient in the majority of the polyherbal preparations while the lowest value was recorded for copper. However, vitamin C was absent in the herbal preparations while vitamin A and E were detected. These findings indicate that these polyherbal formulations contain the essential mineral nutrients and vitamins that could probably be boosting the immune system of tuberculosis patients.
{"title":"Evaluation of important mineral nutrients and vitamins in polyherbal medicines used for the treatment of tuberculosis in the Eastern Cape Province, South Africa","authors":"E. B. Famewo, A. Clarke, A. Afolayan","doi":"10.5138/09750185.2139","DOIUrl":"https://doi.org/10.5138/09750185.2139","url":null,"abstract":"Polyherbal medicines are widely used for the treatment of various diseases in the developing countries. In order to validate their ability in boosting the immune system of tuberculosis patients, the mineral nutrients and vitamins present were determined. Their nutritive properties were analysed using an inductively coupled plasma optical emission spectrometer, while the vitamins were determined using standardized methods. The polyherbal preparations were found to be rich in mineral nutrients and vitamins. Calcium was the highest mineral nutrient detected, while the lowest nutrient was phosphorus. Quantitatively, calcium and magnesium contents in the remedies ranged from 973.30 to 6503.30 mg/100g and 80.00 to 406.00 mg/100g respectively. The amount of phosphorus and potassium was between 20.00 and 263.30 mg/100g; 160.00 and 2050.00 mg/100g respectively. Micro nutrients such as iron, manganese, zinc, aluminium and copper were also detected. Iron was the highest nutrient in the majority of the polyherbal preparations while the lowest value was recorded for copper. However, vitamin C was absent in the herbal preparations while vitamin A and E were detected. These findings indicate that these polyherbal formulations contain the essential mineral nutrients and vitamins that could probably be boosting the immune system of tuberculosis patients.","PeriodicalId":14199,"journal":{"name":"International Journal of Phytomedicine","volume":"20 1","pages":"16-22"},"PeriodicalIF":0.0,"publicationDate":"2018-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83356066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The objective of present study was to evaluate preliminary phytoconstitutents and invitro antioxidant potential of Pentatropis nivalis (Asclepiadaceae). During the preliminary phytochemical analysis, methanolic and aqueous extracts of aerial part of Pentatropis nivalis was screened for the presence of phenolic, saponins, flavonoids, alkaloids, tannins and phytosterols. TLC of extracts were performed by using various solvent systems. Phytochemicals screening and TLC spots of MEPN and AEPN showed the presence of glycoside, steroids, terpenoids, phenolic, saponins, Methanolic extract showed better qualitative tests for presence of secondary metabolites than aqueous extract.The in-vitro antioxidant potential of extracts were evaluated by DPPH and FRAP, and both methods showed that the plant possesses good antioxidant activity.
{"title":"Phytochemical composition and in vitro antioxidant activity of methanolic and aqueous extracts of aerial part of Pentatropis nivalis (Asclepiadaceae)","authors":"Nilesh P. Babre, T. Gouda, N. L. Gowrishankar","doi":"10.5138/09750185.2219","DOIUrl":"https://doi.org/10.5138/09750185.2219","url":null,"abstract":"The objective of present study was to evaluate preliminary phytoconstitutents and invitro antioxidant potential of Pentatropis nivalis (Asclepiadaceae). During the preliminary phytochemical analysis, methanolic and aqueous extracts of aerial part of Pentatropis nivalis was screened for the presence of phenolic, saponins, flavonoids, alkaloids, tannins and phytosterols. TLC of extracts were performed by using various solvent systems. Phytochemicals screening and TLC spots of MEPN and AEPN showed the presence of glycoside, steroids, terpenoids, phenolic, saponins, Methanolic extract showed better qualitative tests for presence of secondary metabolites than aqueous extract.The in-vitro antioxidant potential of extracts were evaluated by DPPH and FRAP, and both methods showed that the plant possesses good antioxidant activity.","PeriodicalId":14199,"journal":{"name":"International Journal of Phytomedicine","volume":"74 1","pages":"68-72"},"PeriodicalIF":0.0,"publicationDate":"2018-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80928796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plants are a natural source of secondary metabolites having antioxidant property and these compounds protect cells against adverse effects of reactive oxygen species. In the present study the methanolic extracts of bark and leaf of medicinal plant - Quassia indica (Gaertn.) Nooteb. were evaluated and compared employing ABTS cation decolorization test, DPPH scavenging assay, reducing power and scavenging potency towards hydrogen peroxide, nitric oxide and super oxide. The results suggested that the bark extract has better antioxidant efficacy than leaf for all in vitro models compared. The IC 50 metrics (mg/g) of bark and leaf for the assays were ABTS (bark 552.36; leaf 1917); DPPH (bark 572.30; leaf 1782.31); H 2 O 2 (bark 566.89; leaf 1904.32); Nitric Oxide (bark 956.75; leaf 1711.74) and Superoxide (bark 235; leaf 506.68) respectively. Results of the study concluded that Q. indica is a promosing source of antioxidants and could be exploited as a safe and stable natural agent for pharmaceutical preparations.
{"title":"Evaluation of antioxidant potential of methanolic extracts of bark and leaf of Quassia indica (Gaertn.) Nooteb","authors":"R. SudhaBai, P. Anusha","doi":"10.5138/09750185.2198","DOIUrl":"https://doi.org/10.5138/09750185.2198","url":null,"abstract":"Plants are a natural source of secondary metabolites having antioxidant property and these compounds protect cells against adverse effects of reactive oxygen species. In the present study the methanolic extracts of bark and leaf of medicinal plant - Quassia indica (Gaertn.) Nooteb. were evaluated and compared employing ABTS cation decolorization test, DPPH scavenging assay, reducing power and scavenging potency towards hydrogen peroxide, nitric oxide and super oxide. The results suggested that the bark extract has better antioxidant efficacy than leaf for all in vitro models compared. The IC 50 metrics (mg/g) of bark and leaf for the assays were ABTS (bark 552.36; leaf 1917); DPPH (bark 572.30; leaf 1782.31); H 2 O 2 (bark 566.89; leaf 1904.32); Nitric Oxide (bark 956.75; leaf 1711.74) and Superoxide (bark 235; leaf 506.68) respectively. Results of the study concluded that Q. indica is a promosing source of antioxidants and could be exploited as a safe and stable natural agent for pharmaceutical preparations.","PeriodicalId":14199,"journal":{"name":"International Journal of Phytomedicine","volume":"40 1","pages":"39-44"},"PeriodicalIF":0.0,"publicationDate":"2018-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78666675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present study was executed to evaluate the myocardial protective effect of methanol extract of Bougainvillea glabra against isoproterenol induced myocardial necrosis in rats. Myocardial necrosis was induced by subcutaneous injection of isoproterenol (85mg/kg body weight) on 29 th and 30 th day at an interval of 24 hours. Myocardial necrosis was evident from the changes of marker enzymes in serum, plasma and heart tissue. The activities of serum cardiac marker enzymes such as lactate dehydrogenase (LDH), creatine kinase myoglobin (CK-MB), serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), triglycerides (TG), total cholesterol (TC), high density lipoproteins (HDL), low density lipoproteins (LDL), very low density lipoproteins (VLDL) and total protein (TP) were estimated. In addition, plasma TBARS and plasma LDH levels were also recorded. Antioxidant parameters viz catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), glutathione peroxidase (GPx) and malondialdehyde (MDA) levels were performed in heart tissue homogenate. The outcome of the study indicated that, pretreatment with methanol extract of Bougainvillea glabra to isoproterenol induced rats significantly prevented the altered serum cardiac marker enzymes, plasma levels and antioxidant parameters to near normal status. The cardioprotective effect was compared with propranolol (10 mg/kg, oral) which was used as the standard. Histopathological findings exposed a reduced degree of necrosis and inflammation succeeding pretreatment with Bougainvillea glabra. Based on these results, it was suggested that methanol extract of Bougainvillea glabra prevents myocardial necrosis and oxidative stress induced by isoproterenol.
{"title":"Cardioprotective and antioxidant effects of Bougainvillea glabra against isoproterenol induced myocardial necrosis in albino rats","authors":"R. G. Krishna, R. Rajan","doi":"10.5138/09750185.2200","DOIUrl":"https://doi.org/10.5138/09750185.2200","url":null,"abstract":"The present study was executed to evaluate the myocardial protective effect of methanol extract of Bougainvillea glabra against isoproterenol induced myocardial necrosis in rats. Myocardial necrosis was induced by subcutaneous injection of isoproterenol (85mg/kg body weight) on 29 th and 30 th day at an interval of 24 hours. Myocardial necrosis was evident from the changes of marker enzymes in serum, plasma and heart tissue. The activities of serum cardiac marker enzymes such as lactate dehydrogenase (LDH), creatine kinase myoglobin (CK-MB), serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), triglycerides (TG), total cholesterol (TC), high density lipoproteins (HDL), low density lipoproteins (LDL), very low density lipoproteins (VLDL) and total protein (TP) were estimated. In addition, plasma TBARS and plasma LDH levels were also recorded. Antioxidant parameters viz catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), glutathione peroxidase (GPx) and malondialdehyde (MDA) levels were performed in heart tissue homogenate. The outcome of the study indicated that, pretreatment with methanol extract of Bougainvillea glabra to isoproterenol induced rats significantly prevented the altered serum cardiac marker enzymes, plasma levels and antioxidant parameters to near normal status. The cardioprotective effect was compared with propranolol (10 mg/kg, oral) which was used as the standard. Histopathological findings exposed a reduced degree of necrosis and inflammation succeeding pretreatment with Bougainvillea glabra. Based on these results, it was suggested that methanol extract of Bougainvillea glabra prevents myocardial necrosis and oxidative stress induced by isoproterenol.","PeriodicalId":14199,"journal":{"name":"International Journal of Phytomedicine","volume":"910 1","pages":"45-57"},"PeriodicalIF":0.0,"publicationDate":"2018-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77509479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Human Beings have been using plant products to heal the Wounds and Diseases from the inception of humankind. Even when it was not known that microorganisms exist, People have been using antimicrobial agents prepared from plants. These antimicrobial products were prepared by extracting the plant in a suitable solvent. Antimicrobial property is conferred to plants by the presence of various phytochemicals which are the products of several Secondary metabolic pathways. The aim of this project was to decipher the potential use of Daucus carota ssp. Sativus and Butea monosperma in the pharmaceutical industry. In this research, Qualitative phytochemical screening and antimicrobial potential of Black carrot and Kamarkas has been studied. Black carrot showed good antimicrobial activity against A. brasiliensis, E. coli and S. enterica , arranged in descending order of the Slope obtained in each antimicrobial assay. Phytochemical screening showed the presence of Flavonoids, Soluble Phenolic Compounds, Naphthoquinone and traces of Saponins and Alkaloids. The Kamarkas showed antimicrobial activity against S. aureus and to some extent against A. brasiliensis . Phytochemical analysis of Kamarkas showed positive for all phytochemicals.
{"title":"Study of Daucus carota ssp. Sativus and Butea monosperma to analyse their Applicability in Pharmaceutical Industry as Antimicrobial Agents","authors":"N. Bhalla, Vijay Kumar, S. Shukla","doi":"10.5138/09750185.2178","DOIUrl":"https://doi.org/10.5138/09750185.2178","url":null,"abstract":"Human Beings have been using plant products to heal the Wounds and Diseases from the inception of humankind. Even when it was not known that microorganisms exist, People have been using antimicrobial agents prepared from plants. These antimicrobial products were prepared by extracting the plant in a suitable solvent. Antimicrobial property is conferred to plants by the presence of various phytochemicals which are the products of several Secondary metabolic pathways. The aim of this project was to decipher the potential use of Daucus carota ssp. Sativus and Butea monosperma in the pharmaceutical industry. In this research, Qualitative phytochemical screening and antimicrobial potential of Black carrot and Kamarkas has been studied. Black carrot showed good antimicrobial activity against A. brasiliensis, E. coli and S. enterica , arranged in descending order of the Slope obtained in each antimicrobial assay. Phytochemical screening showed the presence of Flavonoids, Soluble Phenolic Compounds, Naphthoquinone and traces of Saponins and Alkaloids. The Kamarkas showed antimicrobial activity against S. aureus and to some extent against A. brasiliensis . Phytochemical analysis of Kamarkas showed positive for all phytochemicals.","PeriodicalId":14199,"journal":{"name":"International Journal of Phytomedicine","volume":"11 1","pages":"11-15"},"PeriodicalIF":0.0,"publicationDate":"2018-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80841610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fabiana Garbachi de Oliveira Mendes Ouri, P. Caruso, Gabriela Silva, H. B. Dias, J. R. Marques, F. B. Nunes, P. M. Ferreira, Jarbas R. Oliveira
Liver fibrosis is a complex disease that is caused by inappropriate tissue repair due to the deposition of connective tissue. When a chronic lesion affects the liver, regenerative response fails and hepatocytes are replaced with abundant extracellular matrix (ECM). The imbalance between production and degradation of ECM will result in the accumulation of proteins that change normal liver architecture, and thus its functionality. The main source of ECM is the activated hepatic stellate cell (HSC). In order, to clarify possible therapeutic approaches to the disease, this work aimed to evaluate the possible antifibrotic action of Pluchea sagitallis (Lam.) Cabrera on an activated HSC immortalized lineage (GRX). Our results demonstrated that the P. sagittalis aqueous extract at 0.039 and 0.078 mg/mL concentrations was able to reduce cell growth and proliferation. Regarding to oxidative stress evaluation, there was no statistically significant difference between the treated group and the control. Staining with OilRed-O (ORO) showed a statistically significant increase in intracellular lipid content after 5 days of treatment, exerting in vitro effect on the GRX phenotypic change of activated towards the quiescent state. These results were confirmed by colorimetric quantification of lipid content. Regarding the TGF-β1 and collagen production, there were no statistically significant differences observed between the groups. In conclusion, the P. sagittalis aqueous extract reduces the growth and proliferation of GRX cells and induces the reversal of activated towards a quiescent phenotype. There was no decrease in cell proliferation either by necrosis or by apoptosis via activation of the senescence. Thus, our data suggest that the extract showed an antifibrotic effect, possibly by activating phenotype reversal.
{"title":"Antifibrotic effect of Pluchea sagitallis (Lam.) cabrera aqueous extract in grx cell lineage","authors":"Fabiana Garbachi de Oliveira Mendes Ouri, P. Caruso, Gabriela Silva, H. B. Dias, J. R. Marques, F. B. Nunes, P. M. Ferreira, Jarbas R. Oliveira","doi":"10.5138/09750185.1832","DOIUrl":"https://doi.org/10.5138/09750185.1832","url":null,"abstract":"Liver fibrosis is a complex disease that is caused by inappropriate tissue repair due to the deposition of connective tissue. When a chronic lesion affects the liver, regenerative response fails and hepatocytes are replaced with abundant extracellular matrix (ECM). The imbalance between production and degradation of ECM will result in the accumulation of proteins that change normal liver architecture, and thus its functionality. The main source of ECM is the activated hepatic stellate cell (HSC). In order, to clarify possible therapeutic approaches to the disease, this work aimed to evaluate the possible antifibrotic action of Pluchea sagitallis (Lam.) Cabrera on an activated HSC immortalized lineage (GRX). Our results demonstrated that the P. sagittalis aqueous extract at 0.039 and 0.078 mg/mL concentrations was able to reduce cell growth and proliferation. Regarding to oxidative stress evaluation, there was no statistically significant difference between the treated group and the control. Staining with OilRed-O (ORO) showed a statistically significant increase in intracellular lipid content after 5 days of treatment, exerting in vitro effect on the GRX phenotypic change of activated towards the quiescent state. These results were confirmed by colorimetric quantification of lipid content. Regarding the TGF-β1 and collagen production, there were no statistically significant differences observed between the groups. In conclusion, the P. sagittalis aqueous extract reduces the growth and proliferation of GRX cells and induces the reversal of activated towards a quiescent phenotype. There was no decrease in cell proliferation either by necrosis or by apoptosis via activation of the senescence. Thus, our data suggest that the extract showed an antifibrotic effect, possibly by activating phenotype reversal.","PeriodicalId":14199,"journal":{"name":"International Journal of Phytomedicine","volume":"21 1","pages":"30-38"},"PeriodicalIF":0.0,"publicationDate":"2018-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81205265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lantana rhodesiensis Moldenke, Lippia chevalieri Moldenke, Vitex diversifolia Bak and Vitex doniana Sweet are four species of Verbenaceae family widely used in traditional medicines in Burkina. The aim of this investigation was to study the chromatographic profile of phenols acids and that of the flavonoids as well as the antioxidants activities. Then, three types of extraction were made: acetonic (100%), decoction, ethanol-water(80/20 + 20/80); and fractions of ethanol-water which arehexane fraction (HF), ethyl acetate fraction (EAF), butanol fraction (BF) and aqueous fraction (AqF) of each species. These evaluations were supplemented by the quantification of the metal ions and the characterization by atomic absorption and HPLC-DAD methods of totals phenols acids and totals flavonoids.The whole of these proportioning was directed against the antioxidant activity through the anti-DPPH* (free radical scavenging activity), anti-TAC (total antioxidant capacity) and anti-IRP(iron reducing power).36 flavonoids on 41 were highlighted by the HPLC-DAD method. A derivative glycosylate of quercetin was detected in the V. diversifolia extracts. L. chevalieri presented the strongest value in terms of ions content with a 55.88 g/kg of calcium.In terms of antioxidant activity, L. rhodesiensis and L. chevalieri presented the best activities on total antioxidant activity and anti-DPPH, and iron reducing power respectively.It’s arise that decoction extract have the best’s activities and can justify the traditional uses.
{"title":"Phytochemistry investigation and antioxidant activity of four edible Verbenaceae of Burkina Faso","authors":"Bangou Mindiédiba Jean, Reyes-Martínez Alfonso, Coulidiati Tangbadioa Hervé, Megalizzi Véronique, Nacoulma Odile Germaine","doi":"10.5138/09750185.2192","DOIUrl":"https://doi.org/10.5138/09750185.2192","url":null,"abstract":"Lantana rhodesiensis Moldenke, Lippia chevalieri Moldenke, Vitex diversifolia Bak and Vitex doniana Sweet are four species of Verbenaceae family widely used in traditional medicines in Burkina. The aim of this investigation was to study the chromatographic profile of phenols acids and that of the flavonoids as well as the antioxidants activities. Then, three types of extraction were made: acetonic (100%), decoction, ethanol-water(80/20 + 20/80); and fractions of ethanol-water which arehexane fraction (HF), ethyl acetate fraction (EAF), butanol fraction (BF) and aqueous fraction (AqF) of each species. These evaluations were supplemented by the quantification of the metal ions and the characterization by atomic absorption and HPLC-DAD methods of totals phenols acids and totals flavonoids.The whole of these proportioning was directed against the antioxidant activity through the anti-DPPH* (free radical scavenging activity), anti-TAC (total antioxidant capacity) and anti-IRP(iron reducing power).36 flavonoids on 41 were highlighted by the HPLC-DAD method. A derivative glycosylate of quercetin was detected in the V. diversifolia extracts. L. chevalieri presented the strongest value in terms of ions content with a 55.88 g/kg of calcium.In terms of antioxidant activity, L. rhodesiensis and L. chevalieri presented the best activities on total antioxidant activity and anti-DPPH, and iron reducing power respectively.It’s arise that decoction extract have the best’s activities and can justify the traditional uses.","PeriodicalId":14199,"journal":{"name":"International Journal of Phytomedicine","volume":"6 1","pages":"655-672"},"PeriodicalIF":0.0,"publicationDate":"2017-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84988920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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