Pub Date : 2011-12-01DOI: 10.5145/KJCM.2011.14.4.153
Jayoung Kim, Y. Park, Soyoung Shin, Yonggoo Kim, S. Park
Elizabethkingia meningoseptica (Chryseobacterium meningoseptica) is a ubiquitous Gram-negative bacillus in the natural and hospital environments. This microorganism causes neonatal meningitis but rarely causes infections in adults, with most adult cases occurring in severely immunocompromised patients. Since E. meningoseptica is inherently resistant to the usual empiric therapy aimed at Gram-negative bacilli and MIC breakpoints for resistance and susceptibility of E. meningoseptica have not been established by the Clinical and Laboratory Standards Institute, it is very difficult to select effective antibiotics for the treatment of E. meningoseptica infection. We report here three cases of E. meningoseptica isolates (two from blood and one from CSF) from adult patients admitted to Seoul St. Mary’s hospital over a 3-year period. To the best of our knowledge, this is the first report of adult meningitis due to E. meningoseptica in Korea (Korean J Clin Microbiol 2011;14:153-157)
elizabeth ethkingia meningoseptica(脑膜炎败血症黄杆菌)是一种在自然和医院环境中普遍存在的革兰氏阴性杆菌。这种微生物引起新生儿脑膜炎，但很少引起成人感染，大多数成人病例发生在严重免疫功能低下的患者中。由于脑膜炎脓毒杆菌对通常针对革兰氏阴性杆菌的经验治疗具有固有的耐药性，而临床与实验室标准研究所尚未建立脑膜炎脓毒杆菌耐药和敏感的MIC断点，因此选择有效的抗生素治疗脑膜炎脓毒杆菌感染非常困难。我们在此报告三例脑膜炎脓毒杆菌分离病例(两例来自血液，一例来自脑脊液)，来自首尔圣玛丽医院住院的成年患者，时间超过3年。据我们所知，这是韩国首例由脑膜炎脓毒杆菌引起的成人脑膜炎报告(Korean J clinclinmicrobiol 2011;14:153-157)。
{"title":"Three Adult Cases of Elizabethkingia meningoseptica Infection in a Korean Hospital","authors":"Jayoung Kim, Y. Park, Soyoung Shin, Yonggoo Kim, S. Park","doi":"10.5145/KJCM.2011.14.4.153","DOIUrl":"https://doi.org/10.5145/KJCM.2011.14.4.153","url":null,"abstract":"Elizabethkingia meningoseptica (Chryseobacterium meningoseptica) is a ubiquitous Gram-negative bacillus in the natural and hospital environments. This microorganism causes neonatal meningitis but rarely causes infections in adults, with most adult cases occurring in severely immunocompromised patients. Since E. meningoseptica is inherently resistant to the usual empiric therapy aimed at Gram-negative bacilli and MIC breakpoints for resistance and susceptibility of E. meningoseptica have not been established by the Clinical and Laboratory Standards Institute, it is very difficult to select effective antibiotics for the treatment of E. meningoseptica infection. We report here three cases of E. meningoseptica isolates (two from blood and one from CSF) from adult patients admitted to Seoul St. Mary’s hospital over a 3-year period. To the best of our knowledge, this is the first report of adult meningitis due to E. meningoseptica in Korea (Korean J Clin Microbiol 2011;14:153-157)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128861327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-12-01DOI: 10.5145/KJCM.2011.14.4.138
M. Jung, W. Lee, Myungsun Park
Background: Asymptomatic vancomycin-resistant enterococci (VRE) colonization precedes infection. VREcolonized patients serve as silent reservoirs of enterococci that go on to colonize other patients. Rapidly identifying colonized patients is crucial to prevent the spread of VRE. The culture-based method of VRE screening is time-consuming. We evaluated the diagnostic performance of a recently developed multiplex real-time PCR for the detection of VRE. Methods: We obtained 105 rectal swabs from patients who were being monitored for carriage of VRE. After 24 hour incubation of swabs in enterococcosel broth (EB) supplemented with 6 μg/mL vancomycin, multiplex real-time PCR was performed using the Anyplex VanR Real-time Detection (VanR) kit (Seegene, Inc., Seoul, Korea). The results of multiplex real-time PCR were compared to those of culture. We evaluated the specificity and detection limits of multiplex real-time PCR using VanR for VRE. Results: A total of 96/105 (91.4%) samples were VRE positive according to multiplex real-time PCR with EB while 85/105 (80.9%) samples were positive in culture. Eleven discordant results (10.4%) (multiplex real-time PCR positive, culture negative) were noted. All non-enterococcal bacteria and vancomycin-susceptible enterococci were negative. The DNA detection limits of VanR were 0.035 pg per reaction (3 μL) for Enterococcus faecium and 0.35 pg for Enterococcus faecalis. Conclusion: The application of multiplex real-time PCR after EB incubation allows rapid and sensitive detection in 26-28 hours for VRE screening from rectal swabs. This method could facilitate the timely implementation of contact isolation to prevent the spread of VRE. (Korean J Clin Microbiol 2011;14: 138-143)
{"title":"Evaluation of a Newly Developed Multiplex Real-time PCR Assay for the Detection of Vancomycin-Resistant Enterococci from Rectal Swabs","authors":"M. Jung, W. Lee, Myungsun Park","doi":"10.5145/KJCM.2011.14.4.138","DOIUrl":"https://doi.org/10.5145/KJCM.2011.14.4.138","url":null,"abstract":"Background: Asymptomatic vancomycin-resistant enterococci (VRE) colonization precedes infection. VREcolonized patients serve as silent reservoirs of enterococci that go on to colonize other patients. Rapidly identifying colonized patients is crucial to prevent the spread of VRE. The culture-based method of VRE screening is time-consuming. We evaluated the diagnostic performance of a recently developed multiplex real-time PCR for the detection of VRE. Methods: We obtained 105 rectal swabs from patients who were being monitored for carriage of VRE. After 24 hour incubation of swabs in enterococcosel broth (EB) supplemented with 6 μg/mL vancomycin, multiplex real-time PCR was performed using the Anyplex VanR Real-time Detection (VanR) kit (Seegene, Inc., Seoul, Korea). The results of multiplex real-time PCR were compared to those of culture. We evaluated the specificity and detection limits of multiplex real-time PCR using VanR for VRE. Results: A total of 96/105 (91.4%) samples were VRE positive according to multiplex real-time PCR with EB while 85/105 (80.9%) samples were positive in culture. Eleven discordant results (10.4%) (multiplex real-time PCR positive, culture negative) were noted. All non-enterococcal bacteria and vancomycin-susceptible enterococci were negative. The DNA detection limits of VanR were 0.035 pg per reaction (3 μL) for Enterococcus faecium and 0.35 pg for Enterococcus faecalis. Conclusion: The application of multiplex real-time PCR after EB incubation allows rapid and sensitive detection in 26-28 hours for VRE screening from rectal swabs. This method could facilitate the timely implementation of contact isolation to prevent the spread of VRE. (Korean J Clin Microbiol 2011;14: 138-143)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"34 2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129028541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-12-01DOI: 10.5145/KJCM.2011.14.4.148
M. Bae, S. Namgoong, Dongheui An, M. N. Kim, Sung‐Han Kim, K. Park, Sung Gyu Lee
Cryptococcus is an opportunistic pathogen that mainly affects immunocompromised hosts and, less frequently, immunocompetent hosts. It causes serious morbidity and mortality due to systemic infections such as meningoencephalitis and pulmonary infection. Urinary involvement of Cryptococcus is sometimes reported among cases of disseminated cryptococcosis in AIDS patients, but no such reports have been published in Korea. We report two cases of cryptococcuria that developed in a 71-year old female with diabetes and liver cirrhosis and in a 50-year old male who received a liver transplant due to HBV-associated hepatic failure. The female patient had received prednisolone for 12 days before we detected C. neoformans in urine culture. Even though no antifungal therapy was indicated for cryptococcuria, following urine culture became negative, but still positive for cryptococcal antigen on hospital day 25. Her blood, CSF culture, and antigen tests were negative, and therefore she was diagnosed with isolated cryptococcuria. The male patient had received prednisolone and tacrolimus for 10 days before sputum and urine cultures became positive for C. neoformans. He had ill defined nodules and pleural effusion in both lungs on chest CT. His cryptococcuria was sustained for over 2 months, despite receiving amphotericin B treatment. His cryptococcuria seemed to be a symptom of disseminated cryptococcosis. (Korean J Clin Microbiol 2011;14:148-152)
{"title":"Two Cases of Cryptococcuria Developed as Isolated Cryptococcuria and Disseminated Cryptococcosis.","authors":"M. Bae, S. Namgoong, Dongheui An, M. N. Kim, Sung‐Han Kim, K. Park, Sung Gyu Lee","doi":"10.5145/KJCM.2011.14.4.148","DOIUrl":"https://doi.org/10.5145/KJCM.2011.14.4.148","url":null,"abstract":"Cryptococcus is an opportunistic pathogen that mainly affects immunocompromised hosts and, less frequently, immunocompetent hosts. It causes serious morbidity and mortality due to systemic infections such as meningoencephalitis and pulmonary infection. Urinary involvement of Cryptococcus is sometimes reported among cases of disseminated cryptococcosis in AIDS patients, but no such reports have been published in Korea. We report two cases of cryptococcuria that developed in a 71-year old female with diabetes and liver cirrhosis and in a 50-year old male who received a liver transplant due to HBV-associated hepatic failure. The female patient had received prednisolone for 12 days before we detected C. neoformans in urine culture. Even though no antifungal therapy was indicated for cryptococcuria, following urine culture became negative, but still positive for cryptococcal antigen on hospital day 25. Her blood, CSF culture, and antigen tests were negative, and therefore she was diagnosed with isolated cryptococcuria. The male patient had received prednisolone and tacrolimus for 10 days before sputum and urine cultures became positive for C. neoformans. He had ill defined nodules and pleural effusion in both lungs on chest CT. His cryptococcuria was sustained for over 2 months, despite receiving amphotericin B treatment. His cryptococcuria seemed to be a symptom of disseminated cryptococcosis. (Korean J Clin Microbiol 2011;14:148-152)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132081949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-12-01DOI: 10.5145/KJCM.2011.14.4.119
Sunjoo Kim
Serological methods for the epidemiological study of Streptococcus pyogenes, such as T-, or M-typing, were replaced by emm typing in the 2000s. The frequency of emm types may differ according to geographical area and study period. Erythromycin resistance rates and the prevalence of erythromycin-resistant phenotypes in several countries are surveyed, and common emm genotypes associated with erythromycin resistance are described for each country. There is no correlation between erythromycin resistance and macrolide use in Korea. S. pyogenes is thought to cause severe illness, such as streptococcal toxic shock syndrome (STSS) and necrotizing fasciitis. The mortality rate of STSS is about 50%, and there have been several hundred victims of STSS in Japan in the last 2 decades. The resistance rate to macrolides peaked at 50% in 2002, and currently, ＜10% of strains exhibit macrolide resistance in Korea. However, the erythromycin resistance rate recently exceeded 90% in China. Considering increases of travel between neighboring countries, a vigilant survey to monitor these highly virulent and antibiotic-resistant strains is necessary. (Korean J Clin Microbiol 2011;14:119-125)
{"title":"Epidemiology and Erythromycin Resistance of Streptococcus pyogenes in the Last 20 Years","authors":"Sunjoo Kim","doi":"10.5145/KJCM.2011.14.4.119","DOIUrl":"https://doi.org/10.5145/KJCM.2011.14.4.119","url":null,"abstract":"Serological methods for the epidemiological study of Streptococcus pyogenes, such as T-, or M-typing, were replaced by emm typing in the 2000s. The frequency of emm types may differ according to geographical area and study period. Erythromycin resistance rates and the prevalence of erythromycin-resistant phenotypes in several countries are surveyed, and common emm genotypes associated with erythromycin resistance are described for each country. There is no correlation between erythromycin resistance and macrolide use in Korea. S. pyogenes is thought to cause severe illness, such as streptococcal toxic shock syndrome (STSS) and necrotizing fasciitis. The mortality rate of STSS is about 50%, and there have been several hundred victims of STSS in Japan in the last 2 decades. The resistance rate to macrolides peaked at 50% in 2002, and currently, ＜10% of strains exhibit macrolide resistance in Korea. However, the erythromycin resistance rate recently exceeded 90% in China. Considering increases of travel between neighboring countries, a vigilant survey to monitor these highly virulent and antibiotic-resistant strains is necessary. (Korean J Clin Microbiol 2011;14:119-125)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129291042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-09-01DOI: 10.5145/KJCM.2011.14.3.103
Hye-young Wang, Hyunwoo Jin, H. Bang, Yeon Im Choi, E. Park, W. Koh, Hyeyoung Lee
Evaluation of MolecuTech Real MTB-ID for MTB/NTM Detection Using Direct Specimens Hye-young Wang*, Hyunwoo Jin*, Hyeeun Bang, Yeon-Im Choi, Eun-mi Park, Won-jung Koh, Hyeyoung Lee M&D, Inc. Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, YD Diagnostics, Yongin, Division of Pulmonary and Critical Care Medicine, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea
molecular tech Real MTB- id在MTB/NTM直接检测中的应用研究王惠英*，陈贤宇*，方贤恩，崔延仁，朴恩美，高元中，李惠英M&D, Inc。韩国首尔成均馆大学医学院三星医疗中心医学部肺与重症医学科，元州延世大学健康科学学院生物医学实验学系，龙仁YD诊断学
{"title":"Evaluation of MolecuTech Real MTB-ID for MTB/NTM Detection Using Direct Specimens","authors":"Hye-young Wang, Hyunwoo Jin, H. Bang, Yeon Im Choi, E. Park, W. Koh, Hyeyoung Lee","doi":"10.5145/KJCM.2011.14.3.103","DOIUrl":"https://doi.org/10.5145/KJCM.2011.14.3.103","url":null,"abstract":"Evaluation of MolecuTech Real MTB-ID for MTB/NTM Detection Using Direct Specimens Hye-young Wang*, Hyunwoo Jin*, Hyeeun Bang, Yeon-Im Choi, Eun-mi Park, Won-jung Koh, Hyeyoung Lee M&D, Inc. Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, YD Diagnostics, Yongin, Division of Pulmonary and Critical Care Medicine, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121961998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-09-01DOI: 10.5145/KJCM.2011.14.3.97
H. Yun, H. Kim, Young Kyung Lee, H. Kang, Jae Seok Kim, W. Song, K. Lee
Background: The inhibition rates for nucleic acid tests of Mycobacterium tuberculosis have been reported to range from less than 1% to more than 10%. Specimen dilution, boiling, addition of bovine serum albumin (BSA), and a silica membrane can be used to override amplification inhibitors in nucleic acid tests of M. tuberculosis. The inhibition rate for real-time PCR of M. tuberculosis (COBAS TaqMan MTB test; Roche Diagnostics, Manheim, Germany) and effective strategies to override PCR inhibitors were investigated in this study. Methods: The inhibition rate for COBAS TaqMan MTB test was investigated in 980 clinical specimens. The effectiveness of PCR inhibitor removal by repeated run, dilution, boiling, addition of BSA, and use of silica membrane were evaluated in the inhibited specimens. Results: Inhibitory substances were present in 4.1% of specimens (40/980). Among 40 inhibited specimens, inhibitory substances were removed in 12 (30%), 30 (75%), 27 (67.5%), 25 (62.5%) and 12 (30%) specimens with repeated run, dilution, addition of RBS, boiling and use of silica membrane, respectively. Conclusion: The overall inhibition rate for the COBAS TaqMan MTB test was 4.1%. Dilution, boiling and addition of BSA were shown to be more effective than repeated run and use of silica membrane for removal of PCR inhibitors. A combination of two methods might be useful and should be studied in the future. (Korean J Clin Microbiol 2011;14:97-102)
{"title":"Removal of PCR Inhibitors in Real-time PCR for Mycobacterium tuberculosis","authors":"H. Yun, H. Kim, Young Kyung Lee, H. Kang, Jae Seok Kim, W. Song, K. Lee","doi":"10.5145/KJCM.2011.14.3.97","DOIUrl":"https://doi.org/10.5145/KJCM.2011.14.3.97","url":null,"abstract":"Background: The inhibition rates for nucleic acid tests of Mycobacterium tuberculosis have been reported to range from less than 1% to more than 10%. Specimen dilution, boiling, addition of bovine serum albumin (BSA), and a silica membrane can be used to override amplification inhibitors in nucleic acid tests of M. tuberculosis. The inhibition rate for real-time PCR of M. tuberculosis (COBAS TaqMan MTB test; Roche Diagnostics, Manheim, Germany) and effective strategies to override PCR inhibitors were investigated in this study. Methods: The inhibition rate for COBAS TaqMan MTB test was investigated in 980 clinical specimens. The effectiveness of PCR inhibitor removal by repeated run, dilution, boiling, addition of BSA, and use of silica membrane were evaluated in the inhibited specimens. Results: Inhibitory substances were present in 4.1% of specimens (40/980). Among 40 inhibited specimens, inhibitory substances were removed in 12 (30%), 30 (75%), 27 (67.5%), 25 (62.5%) and 12 (30%) specimens with repeated run, dilution, addition of RBS, boiling and use of silica membrane, respectively. Conclusion: The overall inhibition rate for the COBAS TaqMan MTB test was 4.1%. Dilution, boiling and addition of BSA were shown to be more effective than repeated run and use of silica membrane for removal of PCR inhibitors. A combination of two methods might be useful and should be studied in the future. (Korean J Clin Microbiol 2011;14:97-102)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128545754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-09-01DOI: 10.5145/KJCM.2011.14.3.115
Y. Kim, E. Koh, Yong Wha Lee, You Kyoung Lee, H. Shin, Kyungwon Lee
Brevundimonas diminuta is a lactose non-fermenting Gram-negative rod associated with infection in immunocompromised patients. In three patients from two general wards, B. diminuta was isolated in blood culture sample. The clinical features of the patients did not coincide with the blood culture result, and pseudo-outbreak was suspected. These isolated were biochemically identified as Brevundimonas diminuta, and 16S rRNA sequencing confirmed their identification. The PFGE result showed a single pattern, and their clonality was assumed. (Korean J Clin Microbiol 2011;14:115-117)
{"title":"Pseudo-outbreak of Brevundimonas diminuta","authors":"Y. Kim, E. Koh, Yong Wha Lee, You Kyoung Lee, H. Shin, Kyungwon Lee","doi":"10.5145/KJCM.2011.14.3.115","DOIUrl":"https://doi.org/10.5145/KJCM.2011.14.3.115","url":null,"abstract":"Brevundimonas diminuta is a lactose non-fermenting Gram-negative rod associated with infection in immunocompromised patients. In three patients from two general wards, B. diminuta was isolated in blood culture sample. The clinical features of the patients did not coincide with the blood culture result, and pseudo-outbreak was suspected. These isolated were biochemically identified as Brevundimonas diminuta, and 16S rRNA sequencing confirmed their identification. The PFGE result showed a single pattern, and their clonality was assumed. (Korean J Clin Microbiol 2011;14:115-117)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"96 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133653386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-09-01DOI: 10.5145/KJCM.2011.14.3.110
Min Jin Kim, S. Y. Kim, Y. Park, H. Yoon, J. Suh, Hee-Joo Lee
Campylobacter jejuni is one of the important bacterial pathogens causing entero-invasive diarrhea; however, C. jejuni infection is rarely complicated by bacteremia or extra-intestinal localization. In the domestic literature, the majority of the relevant reports have focused on Campylobacter fetus, which causes bacteremia more frequently than enteritis, but there are no reports of C. jejuni bacteremia in Korea. We present the case of a 13-year-old girl who presented with abdominal pain. Blood cultures revealed curved Gram-negative bacilli and small, mucoid, gray colonies on blood agar plates at 37C. Biochemical tests showed oxidase-positive colonies. To confirm the species, 16S rRNA sequence analysis was performed. The isolate exhibited 99.7% homology to C. jejuni subsp. jejuni. The patient was treated with third-generation cephalosporin and aminoglycoside and had negative blood cultures after three days of treatment. She fully recovered within four days with no complications. (Korean J Clin Microbiol 2011;14:110-114)
{"title":"Campylobacter jejuni Bacteremia in a Healthy Child","authors":"Min Jin Kim, S. Y. Kim, Y. Park, H. Yoon, J. Suh, Hee-Joo Lee","doi":"10.5145/KJCM.2011.14.3.110","DOIUrl":"https://doi.org/10.5145/KJCM.2011.14.3.110","url":null,"abstract":"Campylobacter jejuni is one of the important bacterial pathogens causing entero-invasive diarrhea; however, C. jejuni infection is rarely complicated by bacteremia or extra-intestinal localization. In the domestic literature, the majority of the relevant reports have focused on Campylobacter fetus, which causes bacteremia more frequently than enteritis, but there are no reports of C. jejuni bacteremia in Korea. We present the case of a 13-year-old girl who presented with abdominal pain. Blood cultures revealed curved Gram-negative bacilli and small, mucoid, gray colonies on blood agar plates at 37C. Biochemical tests showed oxidase-positive colonies. To confirm the species, 16S rRNA sequence analysis was performed. The isolate exhibited 99.7% homology to C. jejuni subsp. jejuni. The patient was treated with third-generation cephalosporin and aminoglycoside and had negative blood cultures after three days of treatment. She fully recovered within four days with no complications. (Korean J Clin Microbiol 2011;14:110-114)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134298189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-09-01DOI: 10.5145/KJCM.2011.14.3.91
J. Shin, S. Song, M. N. Kim, Sunjoo Kim
Background: Although many laboratories use automated blood culture systems, adequate skin disinfection and optimal blood volume are still critical for successful culture. The authors undertook a nationwide survey to understand the current situation and problems of blood culture in Korea. Methods: A survey of blood culture was performed in March and April 2010, including disinfectants, blood collection intervals, and recommended blood volumes. The laboratory physicians described the storage condition of culture bottles before delivery to the equipment. For quality control, the positive rate and skin contamination rate were studied. Results: Replies to the survey were collected from 74 Korean hospitals. Povidone iodine after either isopropyl alcohol or ethanol application was the most common means of skin disinfection. Sampling of a second set of cultures was performed simultaneously in 38% of hospitals and after a 30-min interval in 50%. The recommended blood volume was 10 mL in most cases (69%), but was 20 mL in 24% of cases. The bottles were stored at 37C before installation in 23% of cases and at room temperature in 16%, whereas 57% were placed directly in the equipment during the night shift. Positive rates ranged 8-10% in 32% of hospitals, 5-8% in 23%, and 3% in 13%. Conclusion: Skin disinfection methods were rather variable. Sampling interval, blood volume, and storage of bottles should be standardized. More than 10% of the hospitals require quality improvement in terms of positive rate and skin contamination rates. (Korean J Clin Microbiol 2011;14:91-96)
{"title":"Nationwide Survey of Blood Culture Performance Regarding Skin Disinfection, Blood Collection and Laboratory Procedures","authors":"J. Shin, S. Song, M. N. Kim, Sunjoo Kim","doi":"10.5145/KJCM.2011.14.3.91","DOIUrl":"https://doi.org/10.5145/KJCM.2011.14.3.91","url":null,"abstract":"Background: Although many laboratories use automated blood culture systems, adequate skin disinfection and optimal blood volume are still critical for successful culture. The authors undertook a nationwide survey to understand the current situation and problems of blood culture in Korea. Methods: A survey of blood culture was performed in March and April 2010, including disinfectants, blood collection intervals, and recommended blood volumes. The laboratory physicians described the storage condition of culture bottles before delivery to the equipment. For quality control, the positive rate and skin contamination rate were studied. Results: Replies to the survey were collected from 74 Korean hospitals. Povidone iodine after either isopropyl alcohol or ethanol application was the most common means of skin disinfection. Sampling of a second set of cultures was performed simultaneously in 38% of hospitals and after a 30-min interval in 50%. The recommended blood volume was 10 mL in most cases (69%), but was 20 mL in 24% of cases. The bottles were stored at 37C before installation in 23% of cases and at room temperature in 16%, whereas 57% were placed directly in the equipment during the night shift. Positive rates ranged 8-10% in 32% of hospitals, 5-8% in 23%, and 3% in 13%. Conclusion: Skin disinfection methods were rather variable. Sampling interval, blood volume, and storage of bottles should be standardized. More than 10% of the hospitals require quality improvement in terms of positive rate and skin contamination rates. (Korean J Clin Microbiol 2011;14:91-96)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130510216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-09-01DOI: 10.5145/KJCM.2011.14.3.85
E. Kim, Eun-Ha Koh, Sunjoo Kim, J. Kang, Jae Seok Kim, J. Shin, N. Lee, Joseph Jeong, J. Cho, Chulhun L. Chang, Young Ree Kim
Department of Laboratory Madicine, Seoul National University College of Medicine, Seoul, Gyeongsang National University School of Medicine, Jinju, Hanyang University College of Medicine, Seoul, Hallym University College of Medicine, Chuncheon, Inje University College of Medicine, Busan, Sungkyunkwan University School of Medicine, Seoul, University of Ulsan College of Medicine, Ulsan, Wonkwang University Medical School, Iksan, Pusan National University School of Medicine, Yangsan, Jeju National University School of Medicine, Jeju, Korea
{"title":"Multicenter Study of Molecular Epidemiology and Antibiotic Resistance of Group A Streptococci in 2008-2009 in Korea","authors":"E. Kim, Eun-Ha Koh, Sunjoo Kim, J. Kang, Jae Seok Kim, J. Shin, N. Lee, Joseph Jeong, J. Cho, Chulhun L. Chang, Young Ree Kim","doi":"10.5145/KJCM.2011.14.3.85","DOIUrl":"https://doi.org/10.5145/KJCM.2011.14.3.85","url":null,"abstract":"Department of Laboratory Madicine, Seoul National University College of Medicine, Seoul, Gyeongsang National University School of Medicine, Jinju, Hanyang University College of Medicine, Seoul, Hallym University College of Medicine, Chuncheon, Inje University College of Medicine, Busan, Sungkyunkwan University School of Medicine, Seoul, University of Ulsan College of Medicine, Ulsan, Wonkwang University Medical School, Iksan, Pusan National University School of Medicine, Yangsan, Jeju National University School of Medicine, Jeju, Korea","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115150555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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