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A Review of Fabrication and Applications of Confined Microchannels for Cell Migration Assay 细胞迁移实验中封闭微通道的制作与应用综述
IF 1.9 4区 工程技术 Q2 Engineering Pub Date : 2024-05-27 DOI: 10.1007/s12541-024-01045-0
Mazlee Bin Mazalan, Ryota Toyohara, Toshiro Ohashi

Cell migration is an essential process in a number of physiological and pathological events, and known to be modulated by external microenvironment because cells may sense physical and chemical signals from the microenvironment and collectively respond to these signals. Over the past two decades, a lot of efforts have been made to study how external microenvironment can affect cell migration behaviors. Cells often migrate through confined environments in vivo, such as extracellular matrices in tissues and capillary vessels. Understanding how cells move in these constrained spaces is crucial to clarify various biological processes. For instance, during embryonic development, cells migrate through specific pathways to form tissues and organs. In wound healing, cells migrate to repair damaged tissues. In cancer, tumour cells migrate to invade surrounding tissues and metastasize to distant sites. Recent advances of bio-MEMS technologies have enabled to characterize cell mechanics and to control local cellular environment at micro-scale. In order to study cell migration under confinement, microchannels have been widely fabricated and used due to their directionality and compatibility. Thus, this study reviews recent work on fabrication of microchannels and their applications to investigate cell migration behaviors, ranging from straight channels to tortuous structures. Challenges and limitations associated with studying cell migration in microchannels are also discussed. Reviewing cell migration in confined environments may provide valuable insights into the underlying mechanisms of cell migration and aid in developing strategies for therapeutic interventions.

细胞迁移是许多生理和病理事件中的一个重要过程,而且已知会受到外部微环境的调控,因为细胞会感知来自微环境的物理和化学信号,并对这些信号做出集体反应。过去二十年来,人们一直致力于研究外部微环境如何影响细胞迁移行为。在体内,细胞经常在受限环境中迁移,如组织中的细胞外基质和毛细血管。了解细胞如何在这些受限空间中移动对阐明各种生物过程至关重要。例如,在胚胎发育过程中,细胞通过特定途径迁移以形成组织和器官。在伤口愈合过程中,细胞迁移以修复受损组织。在癌症中,肿瘤细胞迁移到周围组织并转移到远处。生物微机电系统(Bio-MEMS)技术的最新进展使人们能够在微尺度上描述细胞力学特性并控制局部细胞环境。为了研究封闭条件下的细胞迁移,微通道因其方向性和兼容性而被广泛制造和使用。因此,本研究综述了近期有关微通道制造及其在研究细胞迁移行为中的应用的研究成果,包括直通道和迂回结构。本研究还讨论了与研究微通道中细胞迁移相关的挑战和局限性。研究细胞在封闭环境中的迁移可为了解细胞迁移的基本机制提供有价值的见解,并有助于制定治疗干预策略。
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引用次数: 0
A Study on the Development of Driver Behavior Simulation Dummy for the Performance Evaluation of Driver Monitoring System 为驾驶员监控系统性能评估开发驾驶员行为模拟假人的研究
IF 1.9 4区 工程技术 Q2 Engineering Pub Date : 2024-05-24 DOI: 10.1007/s12541-024-01037-0
Jin Hae Yae, Young Dal Oh, Moon Sik Kim, Sun Hong Park

Driver monitoring system (DMS) was mainly developed to prevent accident risks by analyzing facial movements related to drowsiness and carelessness in real time such as driver’s gaze, blink, and head angle through cameras and warning the driver. Recently, the scope has been expanded to monitor passengers, and it has been linked to safety functions such as neglecting children, empty seats, or controlling airbags on seats with people under safety weight. However, evaluation research for algorithm advancement and performance optimization is relatively insufficient. In addition, the verification system is facing limitations such as personal information protection problems caused by the subject’s face data, errors in reproducing the subject’s drowsy and careless behavior, and differences in behavior according to individual differences. Therefore, as the importance of traffic safety is emphasized, an evaluation tool that can more efficiently and systematically evaluate the performance of DMS is needed. In this study, a driver behavior simulation dummy was developed that can quantitatively control the movement of the driver’s face and upper body. The driver behavior simulation dummy was developed in three stages in the order of function and specification definition, design and manufacture according to specifications, and verification through error tests for each function.

驾驶员监控系统(DMS)主要是通过摄像头实时分析驾驶员的凝视、眨眼和头部角度等与瞌睡和粗心有关的面部动作,并向驾驶员发出警告,从而防范事故风险。最近,其范围已扩大到监控乘客,并与忽视儿童、空座位或控制安全重量以下人员座位上的安全气囊等安全功能联系起来。然而,针对算法改进和性能优化的评估研究相对不足。此外,验证系统还面临着受试者面部数据带来的个人信息保护问题、受试者瞌睡和粗心行为的再现误差、个体差异带来的行为差异等局限性。因此,在强调交通安全重要性的同时,需要一种能更有效、更系统地评估 DMS 性能的评估工具。本研究开发了一种驾驶员行为模拟假人,可定量控制驾驶员面部和上半身的运动。驾驶员行为模拟假人的开发分为三个阶段,依次为功能和规格定义、根据规格进行设计和制造,以及通过各项功能的误差测试进行验证。
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引用次数: 0
Advancements in Biodegradable Printed Circuit Boards: Review of Material Properties, Fabrication Methods, Applications and Challenges 可生物降解印刷电路板的进展:材料特性、制造方法、应用和挑战综述
IF 1.9 4区 工程技术 Q2 Engineering Pub Date : 2024-05-14 DOI: 10.1007/s12541-024-01027-2
Chin Fhong Soon, See Khee Yee, Anis Nurashikin Nordin, Rosminazuin Ab Rahim, Nyuk Ling Ma, Intan Sue Liana Abd Hamed, Kian Sek Tee, Nur Hanisah Azmi, Norshuhaila Mohamed Sunar, Chris Heng

As electronic waste poses environmental challenges, exploring eco-friendly alternatives becomes imperative. In this review, the introduction reveals the disposal problem of existing printed circuit boards (PCBs) and the potential impacts of implementing biodegradable PCBs towards the United Nations Sustainable Development Goals. Various biodegradable materials, including polylactic acid, cellulose/cellulose acetate, silk proteins, gelatin, polyvinyl alcohol, mycelium, and wood, were evaluated for their properties and suitability in PCB manufacturing. Each material is scrutinised for its suitability in creating environmentally friendly circuit boards. The study meticulously analyses these biodegradable PCBs' electrical, mechanical, thermal and decomposition properties, providing insights into their performance under various conditions. The article also explores different fabrication methods and their advantages and limitations for manufacturing biodegradable PCBs. Solvent and non-solvent based decomposition of the biodegradable PCBs were revealed. The research outcome on a balance between hygroscopic property and degradability of biodegradable PCBs is revealed. The narrative extends to encompass the challenges and issues associated with the Design-for-Manufacturing processes and life cycle assessment of biodegradable PCBs, shedding light on potential hurdles and areas for improvement. The article concludes with a forward-looking perspective on the future of biodegradable printed circuit boards, environmentally friendly fire-retardants, a proposal for alternative standards for biodegradable PCBs, and their increasing role in sustainable electronics.

由于电子废物给环境带来了挑战,探索生态友好型替代品势在必行。本综述揭示了现有印刷电路板(PCB)的处置问题,以及采用生物可降解印刷电路板对实现联合国可持续发展目标的潜在影响。对各种可生物降解材料,包括聚乳酸、纤维素/醋酸纤维素、蚕丝蛋白、明胶、聚乙烯醇、菌丝体和木材,进行了性能和是否适合用于印刷电路板制造的评估。对每种材料进行了仔细检查,以确定其是否适用于制造环保电路板。研究细致分析了这些可生物降解印刷电路板的电气、机械、热和分解特性,深入探讨了它们在各种条件下的性能。文章还探讨了制造生物可降解印刷电路板的不同制造方法及其优势和局限性。文章揭示了生物可降解多氯联苯的溶剂和非溶剂分解。揭示了生物可降解多氯联苯吸湿性和降解性之间平衡的研究成果。文章还阐述了与可生物降解多氯联苯的制造工艺设计和生命周期评估相关的挑战和问题,揭示了潜在的障碍和有待改进的领域。文章最后对可生物降解印刷电路板的未来、环境友好型阻燃剂、可生物降解印刷电路板替代标准的建议以及可生物降解印刷电路板在可持续电子产品中日益重要的作用进行了前瞻性展望。
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引用次数: 0
Experimental Analysis of Polishing of Hybrid Aluminium Metal Matrix Composite Reinforced with SiC, ZrO2, and NiTi Particles Using a Developed Rotary Abrasive Float Polishing System 使用开发的旋转式磨料浮动抛光系统抛光用 SiC、ZrO2 和 NiTi 颗粒增强的混合铝金属基复合材料的实验分析
IF 1.9 4区 工程技术 Q2 Engineering Pub Date : 2024-05-06 DOI: 10.1007/s12541-024-01024-5
Saurabh Kumar Maurya, Chander Kant Susheel, Alakesh Manna

Finishing is an essential process after manufacturing of miniature products. The conventional finishing processes can be used to produce good surface in micro domain but effectiveness of these processes is very poor for polishing of ductile, hard and brittle materials. Considering aforementioned, rotary abrasive float polishing set-up has been developed and utilized for polishing of aluminium matrix composite specimens. The effect of abrasive particle size, abrasive concentration, lap rotation and polishing time on surface finish were analysed. Taguchi L18 mixed orthogonal array was engaged for the experimental design and optimization. The surface roughness height (Ra, µm) of the polished specimens were enhanced from 0.437 to 0.049 µm i.e. 88.79%, when experiments were performed at optimal parametric setting. Abrasive particle size, lap rotation and polishing time was found significant factors in deciding surface roughness. Scanning electrode microscopic and optical images confirm the absence of any scratch and roughness peaks on polished surface specimens.

精加工是微型产品制造后的一道重要工序。传统的表面处理工艺可以在微观领域产生良好的表面,但对于韧性、硬度和脆性材料的抛光,这些工艺的效果很差。考虑到上述情况,我们开发了旋转磨料浮动抛光装置,并将其用于铝基复合材料试样的抛光。分析了磨料粒度、磨料浓度、搭接旋转和抛光时间对表面光洁度的影响。采用田口 L18 混合正交阵列进行实验设计和优化。在最佳参数设置下进行实验时,抛光试样的表面粗糙度高度(Ra,µm)从 0.437 µm 提高到了 0.049 µm,即提高了 88.79%。磨料粒度、搭接旋转和抛光时间是决定表面粗糙度的重要因素。扫描电极显微镜和光学图像证实,抛光表面试样上没有任何划痕和粗糙度峰值。
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引用次数: 0
Single and Multi-response Optimization of Scroll Machining Parameters by the Taguchi Method 用田口方法对涡旋加工参数进行单响应和多响应优化
IF 1.9 4区 工程技术 Q2 Engineering Pub Date : 2024-05-04 DOI: 10.1007/s12541-024-01026-3
Xu Dang, Maged Al-Rahawi, Tao Liu, Salah Taresh Abdo Mohammed

The precision of scroll machining plays a crucial role in scroll compressor efficiency. However, the improvement in scroll surface quality usually comes at the cost of an increase in energy consumption, so a trade-off between surface quality and energy consumption is required. Here in, we concentrate on optimizing several scroll end milling parameters including depth of cut, feed rate, cutting speed, and radial depth of cut to enhance the efficiency of scroll compressors, which greatly influence manufacturing responses like cutting force, surface roughness, and machining time. First, a set of scroll milling experiments are conducted and analyzed by the Taguchi L25 orthogonal array. Then, a single response optimization is done by the Taguchi method to show the impact of the milling parameters on each single response. Furthermore, the Taguchi method associated with the desirability function is applied to optimize the multi-response outputs. According to the analysis of variance (ANOVA) for the single-response, feed rate is found the most significant factor, while the results of the multi-response analysis prove that depth of cut with 37.53% is the most significant factor for the entire system. Finally, a quadratic regression analysis is performed to verify the validation of optimized results. The results revealed a 44.04% improvement in machining time, accompanied by a 2.46% enhancement in composite desirability. This demonstrated a perfect fit between the measured and expected values, achieving a balance between surface quality and energy consumption. The optimization results provide a guidance for the high-surface quality machining of scrolls and could be directly applied in the manufacturing processes of scroll compressors.

涡旋加工的精度对涡旋压缩机的效率起着至关重要的作用。然而,涡旋表面质量的提高通常以能耗的增加为代价,因此需要在表面质量和能耗之间进行权衡。在此,我们集中优化了几个涡旋端铣参数,包括切削深度、进给速度、切削速度和径向切削深度,以提高涡旋压缩机的效率,这些参数在很大程度上影响着切削力、表面粗糙度和加工时间等制造响应。首先,进行一组涡旋铣削实验,并通过田口 L25 正交阵列进行分析。然后,采用田口方法进行单响应优化,以显示铣削参数对每个单响应的影响。此外,还采用了与可取函数相关的田口方法来优化多响应输出。根据单响应的方差分析(ANOVA),发现进给率是最重要的因素,而多响应分析的结果证明,切削深度(37.53%)是整个系统中最重要的因素。最后,进行了二次回归分析,以验证优化结果的有效性。结果显示,加工时间缩短了 44.04%,同时复合材料的可取性提高了 2.46%。这表明测量值和预期值完全吻合,实现了表面质量和能耗之间的平衡。优化结果为涡旋的高表面质量加工提供了指导,可直接应用于涡旋压缩机的制造过程。
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引用次数: 0
Biomechanical Evaluation of Modified Ti6Al4V Ligament Anchors with Varied Expansion Directions for Anterior Cruciate Ligament Reconstruction 用于前十字韧带重建的具有不同扩张方向的改良 Ti6Al4V 韧带锚的生物力学评估
IF 1.9 4区 工程技术 Q2 Engineering Pub Date : 2024-04-24 DOI: 10.1007/s12541-024-01005-8
Il Won Suh, S. Jang, Sieb Chanchamnan, Chan Hee Park, Cheol-Sang Kim
{"title":"Biomechanical Evaluation of Modified Ti6Al4V Ligament Anchors with Varied Expansion Directions for Anterior Cruciate Ligament Reconstruction","authors":"Il Won Suh, S. Jang, Sieb Chanchamnan, Chan Hee Park, Cheol-Sang Kim","doi":"10.1007/s12541-024-01005-8","DOIUrl":"https://doi.org/10.1007/s12541-024-01005-8","url":null,"abstract":"","PeriodicalId":14359,"journal":{"name":"International Journal of Precision Engineering and Manufacturing","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140659753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Design Optimization for Enhancing Microstrip Antenna Performances Using Polylactic Acid (PLA) Biopolymer Substrate in Sub-6 GHz Band 使用聚乳酸 (PLA) 生物聚合物基底提高 6 GHz 以下频段微带天线性能的设计优化
IF 1.9 4区 工程技术 Q2 Engineering Pub Date : 2024-04-24 DOI: 10.1007/s12541-024-01010-x
Djamila Ziani, M. Belkheir, M. Rouissat, A. Mokaddem, Ibrahim S. Alsukayti, Samir Brahim Belhouari
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引用次数: 0
Recent Applications of Fiber Bragg Grating Sensors in Humidity and Water Content Detection in Agriculture: A Comprehensive Review of Development, Challenges, and Future Trends 光纤布拉格光栅传感器在农业湿度和含水量检测中的最新应用:发展、挑战和未来趋势综述
IF 1.9 4区 工程技术 Q2 Engineering Pub Date : 2024-04-23 DOI: 10.1007/s12541-024-01015-6
Mukhtar Iderawumi Abdulraheem, Yani Xiong, Wei Zhang, Hongjun Chen, Hao Zhang, Jiandong Hu

There has been a growing interest in using Fiber Bragg Grating (FBG) sensors for the detection of humidity and water content due to their high sensitivity, ease of installation, multiplexing capability, reliability, and resistance to electromagnetic interference. Although, several studies and papers have been published on FBG sensors and their applications in various fields such as construction, geophysical, there is a lack of a consolidated review that specifically focuses on recent developments in agriculture with particular reference soil humidity and water content. Therefore, a comprehensive and up-to-date analysis of the advances, detection mechanisms, challenges, and potential future directions in this field is needed. This paper provides an in-depth analysis and summarizes the fundamental principles, advancements, methodologies, and recent research findings, highlighting the potential application in agriculture, development, challenges, and prospects for FBG-based humidity and water content detection. By utilizing the changes in FBG wavelength or amplitude caused by the presence of humidity, they provide an effective means for real-time monitoring and control of water content. However, challenges remain to be addressed, such as the need for accurate calibration and the potential for drift over time. In this review, we further discuss the strategies and techniques proposed to overcome the highlighted challenges, such as sensor packaging, signal processing algorithms, and calibration procedures, and suggest that further studies could be done to investigates the novel materials with enhanced sensitivity and selectivity, development of miniaturized and wireless FBG sensor system, as well as investigating multiplexing techniques for simultaneous measurement of multiple parameters.

由于光纤布拉格光栅 (FBG) 传感器灵敏度高、易于安装、多路复用能力强、可靠性高、抗电磁干扰能力强,人们对使用光纤布拉格光栅传感器检测湿度和含水量的兴趣日益浓厚。虽然已有多篇关于光纤光栅传感器及其在建筑、地球物理等不同领域的应用的研究报告和论文发表,但缺乏专门针对农业领域最新发展(尤其是土壤湿度和含水量)的综合综述。因此,需要对该领域的进展、检测机制、挑战和潜在的未来方向进行全面的最新分析。本文对基于 FBG 的湿度和含水量检测的基本原理、进展、方法和最新研究成果进行了深入分析和总结,重点介绍了其在农业中的潜在应用、发展、挑战和前景。通过利用湿度引起的 FBG 波长或振幅变化,它们为实时监测和控制含水量提供了有效手段。然而,仍有一些挑战有待解决,如需要精确校准和随时间漂移的可能性。在这篇综述中,我们进一步讨论了为克服突出挑战而提出的策略和技术,如传感器封装、信号处理算法和校准程序,并建议进一步研究具有更高灵敏度和选择性的新型材料,开发微型化和无线 FBG 传感器系统,以及研究同时测量多个参数的复用技术。
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引用次数: 0
Bonding-Free Capillary Microfluidics via a 3D-Printed Railed Microchannel 通过三维打印轨道微通道实现无粘接毛细管微流体技术
IF 1.9 4区 工程技术 Q2 Engineering Pub Date : 2024-04-22 DOI: 10.1007/s12541-024-01013-8
Wonhyung Lee, Hye Jin An, Da Bin Kim, Hojin Kim

Microfluidics is a promising research area that is widely used in biochemical applications. Recently, the commercialization of microfluidic devices composed of economical plastics has been highlighted. Plastic microfluidic devices must contain conformal contacts to construct a completely closed channel that prevents leakage during liquid transport. However, the conventional fabrication (i.e., injection molding and bonding) of plastic microfluidic devices requires empirical expertise, high cost, time-consuming, and complex procedures. This limits its extensive use in the research and development (R&D) phase to take the next steps toward final commercialization. In particular, iterative changes in the channel design typically lead to increased time and cost. This study proposes an easy-to-change and cost-effective fabrication method for 3D-printed microfluidic devices that offer bonding- and leakage-free spontaneous capillary flow (SCF). Locking pillar arrays on upper and lower substrates are simply and reliably assembled using friction forces. Incorporating inherent fabrication errors in 3D printing allows the intended and reproducible assembly gaps between the substrates to be used as microchannels. In addition, a novel side-opened (side-railed) channel geometry is applied to provide both SCF and virtual sidewalls (i.e., capillary barriers) along the microchannel. Finally, the proposed device demonstrates a potential fabrication method that can be utilized as a bridge between the R&D and commercialization phases.

微流控技术是一个前景广阔的研究领域,在生化领域应用广泛。最近,由经济型塑料组成的微流体设备的商业化受到关注。塑料微流体设备必须包含保形接触,以构建一个完全封闭的通道,防止液体传输过程中发生泄漏。然而,塑料微流控装置的传统制造方法(即注射成型和粘接)需要经验丰富的专业技术,成本高、耗时长且程序复杂。这就限制了其在研发(R&D)阶段的广泛应用,使其无法向最终商业化迈出下一步。特别是,通道设计的反复更改通常会导致时间和成本的增加。本研究为三维打印微流体设备提出了一种易于更改且经济高效的制造方法,该方法可提供无粘合和无泄漏的自发毛细管流(SCF)。上下基底上的锁定支柱阵列可利用摩擦力简单可靠地组装起来。在三维打印中加入固有的制造误差,可将基底之间预定的、可重复的装配间隙用作微通道。此外,还采用了新颖的侧开(侧轨)通道几何形状,沿微通道提供 SCF 和虚拟侧壁(即毛细管屏障)。最后,所提出的设备展示了一种潜在的制造方法,可用作研发和商业化阶段之间的桥梁。
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引用次数: 0
A Stroke Model for Straightening Partially Heat-Treated Ball Screws with Complex Mechanical Properties 矫直具有复杂机械特性的部分热处理滚珠丝杠的冲程模型
IF 1.9 4区 工程技术 Q2 Engineering Pub Date : 2024-04-20 DOI: 10.1007/s12541-024-01012-9
Ji-Hyeon Shin, Sang Won Kim, Hae-Sung Yoon

Ball screws are surface heat-treated/hardened to improve their fatigue strength. However, the screws inevitably deform during heating. Thus, an additional straightening process is essential. Pressurization through three-point bending is typically adopted; however, determining the appropriate stroke for certain plastic deformations is challenging, mainly due to the complex mechanical properties of surface heat-treated ball screws. In this study, ball screws composed of S55C with various geometric shapes and heat-treated depths were tested with the aim of constructing a stroke model for precise straightening. Three-point bending experiments showed that the plastic deformation of the ball screws after bending was affected by the ratio of the heat-treated area to the total cross-sectional area, as well as the absolute value of the heat-treated area. Response surfaces were constructed according to the given plastic deformation and geometric shape of the ball screws to predict the desired stroke for straightening. Based on the experimental results, a computational model was built by modeling the screws with two materials. By adjusting the mechanical properties of non-heat-treated and heat-treated areas, the computational model showed similar characteristics to the experimental results. It is expected that our approach will contribute to more precise and effective straightening of partially heat-treated ball screws with complex mechanical properties.

滚珠丝杠经过表面热处理/硬化,以提高其疲劳强度。然而,螺钉在加热过程中不可避免地会发生变形。因此,必须进行额外的矫直处理。通常采用三点弯曲加压;然而,确定某些塑性变形的适当冲程具有挑战性,这主要是由于表面热处理滚珠丝杠具有复杂的机械特性。在这项研究中,我们测试了由不同几何形状和热处理深度的 S55C 组成的滚珠丝杠,目的是构建一个用于精确矫直的冲程模型。三点弯曲实验表明,滚珠丝杠弯曲后的塑性变形受热处理面积与总横截面积之比以及热处理面积绝对值的影响。根据给定的塑性变形和滚珠丝杠的几何形状构建响应曲面,以预测矫直所需的行程。根据实验结果,通过对两种材料的螺钉进行建模,建立了计算模型。通过调整未热处理区域和热处理区域的机械性能,计算模型显示出与实验结果相似的特性。预计我们的方法将有助于对具有复杂机械性能的部分热处理滚珠丝杠进行更精确、更有效的矫直。
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引用次数: 0
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International Journal of Precision Engineering and Manufacturing
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