Pub Date : 2022-04-03DOI: 10.1080/07924259.2022.2073277
D. Sahu, K.P. Singh
ABSTRACT The chemical composition of Syzygium aromaticum essential oil (EO), and its efficacy on the development and metamorphosis of polyphagous pest, Pericallia ricini was assessed. The chemical composition of the S. aromaticum EO was analysed by gas chromatography and mass spectrometry method. The major volatile components present in the EO were eugenol, β-caryophyllene, caryophyllenyl alcohol, α-humulene, clovene, globulol, and eugenyl acetate. For topical bioassay, EO was tested against the sixth (penultimate) and seventh (ultimate) larval instars of P. ricini resulted in the disruption of normal moulting and metamorphosis, leading to various developmental aberrations. The results comprised larval mortality, delayed larval-larval, and larval-pupal ecdysis duration, ecdysial failure, formation of non-viable intermediates, emergence of abnormal adults, reduced pupation, and inhibition of total adult emergence. This study also reports about the juvenoid nature of clove oil for the first time. Our finding demonstrates that S. aromaticum EO has significant negative effects on P. ricini, and suggests that it can be implemented for the successful control of this economically important insect.
{"title":"Developmental inhibitory effect of the Syzygium aromaticum essential oil on the postembryonic stages of a polyphagous pest, Pericallia ricini (Lepidoptera: Arctiidae)","authors":"D. Sahu, K.P. Singh","doi":"10.1080/07924259.2022.2073277","DOIUrl":"https://doi.org/10.1080/07924259.2022.2073277","url":null,"abstract":"ABSTRACT The chemical composition of Syzygium aromaticum essential oil (EO), and its efficacy on the development and metamorphosis of polyphagous pest, Pericallia ricini was assessed. The chemical composition of the S. aromaticum EO was analysed by gas chromatography and mass spectrometry method. The major volatile components present in the EO were eugenol, β-caryophyllene, caryophyllenyl alcohol, α-humulene, clovene, globulol, and eugenyl acetate. For topical bioassay, EO was tested against the sixth (penultimate) and seventh (ultimate) larval instars of P. ricini resulted in the disruption of normal moulting and metamorphosis, leading to various developmental aberrations. The results comprised larval mortality, delayed larval-larval, and larval-pupal ecdysis duration, ecdysial failure, formation of non-viable intermediates, emergence of abnormal adults, reduced pupation, and inhibition of total adult emergence. This study also reports about the juvenoid nature of clove oil for the first time. Our finding demonstrates that S. aromaticum EO has significant negative effects on P. ricini, and suggests that it can be implemented for the successful control of this economically important insect.","PeriodicalId":14482,"journal":{"name":"Invertebrate Reproduction & Development","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43107074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-03DOI: 10.1080/07924259.2022.2075285
Dina Housam Abd El-Monem Ahmed
ABSTRACT The application of nuclear techniques to control one of the most important lepidopteran insect pests: the Egyptian cotton leafworm, Spodoptera littoralis is discussed in this study. All experiments had the objective of finding the dose of gamma radiation capable of causing sterility in the first generations by irradiating the parental generation. For S. littoralis, 6th instar larvae were irradiated with doses of 40, 80, 120 and 160 Gy. The larval survival was 93%, 90%, 88%, 85% and 97% at 40, 80, 120, 160 and 0 Gy, respectively. The pupal survival was 88, 85, 83, 80 and 98% at the same doses. Hence, there was a reduction in survival and emergence to the adult stage after treating S. littoralis larvae with radiation. Also, the latent effects of the tested gamma doses on 6th instar larvae of S. littoralis are recorded. The total number of eggs/female and egg fertility were significantly reduced as compared to control, these effects were increased with increasing the dose. It is concluded that radiation is a promising control method for S. littoralis and other related lepidopteran species.
{"title":"Bioefficacy of gamma radiation on Spodoptera littoralis (Lepidoptera: Noctuidae)","authors":"Dina Housam Abd El-Monem Ahmed","doi":"10.1080/07924259.2022.2075285","DOIUrl":"https://doi.org/10.1080/07924259.2022.2075285","url":null,"abstract":"ABSTRACT The application of nuclear techniques to control one of the most important lepidopteran insect pests: the Egyptian cotton leafworm, Spodoptera littoralis is discussed in this study. All experiments had the objective of finding the dose of gamma radiation capable of causing sterility in the first generations by irradiating the parental generation. For S. littoralis, 6th instar larvae were irradiated with doses of 40, 80, 120 and 160 Gy. The larval survival was 93%, 90%, 88%, 85% and 97% at 40, 80, 120, 160 and 0 Gy, respectively. The pupal survival was 88, 85, 83, 80 and 98% at the same doses. Hence, there was a reduction in survival and emergence to the adult stage after treating S. littoralis larvae with radiation. Also, the latent effects of the tested gamma doses on 6th instar larvae of S. littoralis are recorded. The total number of eggs/female and egg fertility were significantly reduced as compared to control, these effects were increased with increasing the dose. It is concluded that radiation is a promising control method for S. littoralis and other related lepidopteran species.","PeriodicalId":14482,"journal":{"name":"Invertebrate Reproduction & Development","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42449180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-01DOI: 10.1080/07924259.2022.2056092
H. Marco, L. Auerswald, T. G. Matumba, G. Gäde
ABSTRACT Astaxanthin, the dominant carotenoid pigment in the South African spiny lobster, Jasus lalandii, was quantified in haemolymph and tissue extracts by means of reverse phase-high pressure liquid chromatography and analysed with respect to growth and reproduction cycles of adults. Haemolymph, exoskeleton, muscle, gonads and hepatopancreas from both sexes, as well as egg parcels from berried females were collected within days of removing the animals from the ocean. The astaxanthin profile is, therefore, representative of spiny lobsters in the wild. Astaxanthin is significantly more in exoskeleton, ovaries and egg parcels, and is influenced by the ovarian cycle in females: it accumulated in growing oocytes and remained in the extruded eggs ostensibly for protection as antioxidant. Radioactive inulin was used to determine total haemolymph volume of a spiny lobster and the gravimetric contribution of body organs and various tissues to the total weight of these animals were measured: muscle tissue constitutes 33% of the total wet weight of J. lalandii, while haemolymph (22%) and exoskeleton (20%) are other major contributors. For maximal harvesting of astaxanthin from carcases, it would thus, be best to focus only on the exoskeleton with an emphasis on the carapace, which can be easily removed.
{"title":"Distribution of astaxanthin in the spiny lobster Jasus lalandii: trends during biological cycles","authors":"H. Marco, L. Auerswald, T. G. Matumba, G. Gäde","doi":"10.1080/07924259.2022.2056092","DOIUrl":"https://doi.org/10.1080/07924259.2022.2056092","url":null,"abstract":"ABSTRACT Astaxanthin, the dominant carotenoid pigment in the South African spiny lobster, Jasus lalandii, was quantified in haemolymph and tissue extracts by means of reverse phase-high pressure liquid chromatography and analysed with respect to growth and reproduction cycles of adults. Haemolymph, exoskeleton, muscle, gonads and hepatopancreas from both sexes, as well as egg parcels from berried females were collected within days of removing the animals from the ocean. The astaxanthin profile is, therefore, representative of spiny lobsters in the wild. Astaxanthin is significantly more in exoskeleton, ovaries and egg parcels, and is influenced by the ovarian cycle in females: it accumulated in growing oocytes and remained in the extruded eggs ostensibly for protection as antioxidant. Radioactive inulin was used to determine total haemolymph volume of a spiny lobster and the gravimetric contribution of body organs and various tissues to the total weight of these animals were measured: muscle tissue constitutes 33% of the total wet weight of J. lalandii, while haemolymph (22%) and exoskeleton (20%) are other major contributors. For maximal harvesting of astaxanthin from carcases, it would thus, be best to focus only on the exoskeleton with an emphasis on the carapace, which can be easily removed.","PeriodicalId":14482,"journal":{"name":"Invertebrate Reproduction & Development","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45622999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-01DOI: 10.1080/07924259.2022.2043466
Andrea Toso, A. Giangrande
ABSTRACT Reproduction of the terebellid Eupolymnia nesidensis was investigated in a population from the Ionian Sea (Mediterranean, South Italy). This species has a worldwide distribution and is very common on Mediterranean hard substrata covered by algae. Observations revealed a spawning period across springtime with lecithotrophic development occurring inside a jelly mass, with eggs measuring 210 μm in diameter. Juveniles leave the jelly mass after 12–13 days building a transitory tube and exhibiting a vagile benthic behaviour, incorporating food directly through the mouth opening. During the juvenile stage, at day ~100, an ontogenetic shift related to feeding behaviour was observed. Specifically, the vagile feeding behaviour was replaced by the adult feeding strategy with the use of the fully developed head tentacles.
{"title":"Development and ontogenetic changes of feeding behaviour during juvenile early growth of Eupolymnia nesidensis (Annelida, Terebellidae)","authors":"Andrea Toso, A. Giangrande","doi":"10.1080/07924259.2022.2043466","DOIUrl":"https://doi.org/10.1080/07924259.2022.2043466","url":null,"abstract":"ABSTRACT Reproduction of the terebellid Eupolymnia nesidensis was investigated in a population from the Ionian Sea (Mediterranean, South Italy). This species has a worldwide distribution and is very common on Mediterranean hard substrata covered by algae. Observations revealed a spawning period across springtime with lecithotrophic development occurring inside a jelly mass, with eggs measuring 210 μm in diameter. Juveniles leave the jelly mass after 12–13 days building a transitory tube and exhibiting a vagile benthic behaviour, incorporating food directly through the mouth opening. During the juvenile stage, at day ~100, an ontogenetic shift related to feeding behaviour was observed. Specifically, the vagile feeding behaviour was replaced by the adult feeding strategy with the use of the fully developed head tentacles.","PeriodicalId":14482,"journal":{"name":"Invertebrate Reproduction & Development","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41296033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-13DOI: 10.1080/07924259.2022.2039308
E. Sokolova, Julia K. Zograf, V. V. Yushin
ABSTRACT Sperm ultrastructure of the hairworm Gordionus alpestris (Nematomorpha, Chordodidae) was studied. Spermatozoa are elongated aflagellate cells of clavate shape. Their dilated anterior part consists of an acrosomal complex comprising an apical acrosomal vesicle and axial acrosomal tube enveloped by an acrosomal sheath of four membrane-bound compartments. The basal part of the tube contains a cluster of vesicles. The slender posterior part of the spermatozoa includes an axial nucleus without a nuclear envelope surrounded by a halo and multivesicular complex composed of cisternae arranged into three layers. Spermatozoa of G. alpestris are nearly identical in size, proportion and internal structure to those of other gordiid spermatozoa. This uniformity makes spermatozoa useless for taxon descriptions and differentiation of Gordiida. The basic pattern of spermatozoon structure in Gordiida is not fully understood and requires more detailed studies. Controversies include absence or presence of the nuclear envelope and mitochondria, together with interpretation of the halo surrounding nuclei and membranous compartments of the acrosomal and multivesicular complexes. Detailed study of sperm development together with cytochemical analysis of components such as mitochondria, acrosome, perforatorium and halo might be helpful for understanding sperm relationships of Gordiida and Nematoda.
{"title":"Ultrastructure of spermatozoa of a hairworm Gordionus alpestris (Villot, 1885) (Nematomorpha, Chordodidae)","authors":"E. Sokolova, Julia K. Zograf, V. V. Yushin","doi":"10.1080/07924259.2022.2039308","DOIUrl":"https://doi.org/10.1080/07924259.2022.2039308","url":null,"abstract":"ABSTRACT Sperm ultrastructure of the hairworm Gordionus alpestris (Nematomorpha, Chordodidae) was studied. Spermatozoa are elongated aflagellate cells of clavate shape. Their dilated anterior part consists of an acrosomal complex comprising an apical acrosomal vesicle and axial acrosomal tube enveloped by an acrosomal sheath of four membrane-bound compartments. The basal part of the tube contains a cluster of vesicles. The slender posterior part of the spermatozoa includes an axial nucleus without a nuclear envelope surrounded by a halo and multivesicular complex composed of cisternae arranged into three layers. Spermatozoa of G. alpestris are nearly identical in size, proportion and internal structure to those of other gordiid spermatozoa. This uniformity makes spermatozoa useless for taxon descriptions and differentiation of Gordiida. The basic pattern of spermatozoon structure in Gordiida is not fully understood and requires more detailed studies. Controversies include absence or presence of the nuclear envelope and mitochondria, together with interpretation of the halo surrounding nuclei and membranous compartments of the acrosomal and multivesicular complexes. Detailed study of sperm development together with cytochemical analysis of components such as mitochondria, acrosome, perforatorium and halo might be helpful for understanding sperm relationships of Gordiida and Nematoda.","PeriodicalId":14482,"journal":{"name":"Invertebrate Reproduction & Development","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41921091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-27DOI: 10.1080/07924259.2022.2043467
G. Montesanto, F. Riva
ABSTRACT The postmarsupial manca stages of Armadillidium ficalbii Arcangeli, 1911 were studied in detail by morphological analysis. Ovigerous females were reared separately under controlled conditions, allowing us to follow the stages of development from release from the marsupium until the appearance of the first juvenile stage, identified by the full development of the seventh pair of pereopods. Each newborn was followed to record the subsequent moults that identify the three postmarsupial manca stages. Manca stage M I had a mean duration of 10 h, manca stage M II 8–9 days, and manca stage M III 9–10 days. The cephalothorax width was measured to provide a growth measure for each stage. The mean values of the cephalothorax width were: 0.589 mm for M I, 0.718 mm for M II and 1.274 mm for M III. The morphological modifications in the three postmarsupial manca stages were described, the body parts illustrated, and SEM images taken. The distinguishing characteristics among mancas were discussed, and comparisons made with manca stages of other terrestrial isopod species.
{"title":"Description of the postmarsupial manca stages of Armadillidium ficalbii (Crustacea, Isopoda, Oniscidea)","authors":"G. Montesanto, F. Riva","doi":"10.1080/07924259.2022.2043467","DOIUrl":"https://doi.org/10.1080/07924259.2022.2043467","url":null,"abstract":"ABSTRACT The postmarsupial manca stages of Armadillidium ficalbii Arcangeli, 1911 were studied in detail by morphological analysis. Ovigerous females were reared separately under controlled conditions, allowing us to follow the stages of development from release from the marsupium until the appearance of the first juvenile stage, identified by the full development of the seventh pair of pereopods. Each newborn was followed to record the subsequent moults that identify the three postmarsupial manca stages. Manca stage M I had a mean duration of 10 h, manca stage M II 8–9 days, and manca stage M III 9–10 days. The cephalothorax width was measured to provide a growth measure for each stage. The mean values of the cephalothorax width were: 0.589 mm for M I, 0.718 mm for M II and 1.274 mm for M III. The morphological modifications in the three postmarsupial manca stages were described, the body parts illustrated, and SEM images taken. The distinguishing characteristics among mancas were discussed, and comparisons made with manca stages of other terrestrial isopod species.","PeriodicalId":14482,"journal":{"name":"Invertebrate Reproduction & Development","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41932279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-16DOI: 10.1080/07924259.2022.2038289
J. Hamel, A. Mercier
ABSTRACT While larval ecology is central to the population dynamics of marine benthic species with multiphasic life histories, the adaptations of larvae to environmental variability remain incompletely understood in many species. Using the holothuroid echinoderm (sea cucumber) Isostichopus fuscus, we examined how a planktotrophic species can use pre-competency plasticity to withstand suboptimal conditions of temperature over extended periods. Five cohorts of propagules (embryos/larvae) were exposed to temperature treatments (28–29, 24–25 and 20–22°C) in standardized culture settings. Under the coldest temperatures (typical of offshore upwelling areas), development mirrored that obtained under warmer temperatures until the early auricularia stage, where development was arrested for ~5 months in a manner consistent with quiescence. When temperature was increased again, larvae quickly resumed development and settled; no lasting negative impact was noted. The findings suggest that the larvae can use a form of dormancy to withstand suboptimal oceanic conditions for indefinite periods of time until successful delivery to appropriate inshore locations, the habitat were I. fuscus is exclusively found.
{"title":"Experimental evidence suggesting temperature-driven dormancy in the planktotrophic larvae of the sea cucumber Isostichopus fuscus","authors":"J. Hamel, A. Mercier","doi":"10.1080/07924259.2022.2038289","DOIUrl":"https://doi.org/10.1080/07924259.2022.2038289","url":null,"abstract":"ABSTRACT While larval ecology is central to the population dynamics of marine benthic species with multiphasic life histories, the adaptations of larvae to environmental variability remain incompletely understood in many species. Using the holothuroid echinoderm (sea cucumber) Isostichopus fuscus, we examined how a planktotrophic species can use pre-competency plasticity to withstand suboptimal conditions of temperature over extended periods. Five cohorts of propagules (embryos/larvae) were exposed to temperature treatments (28–29, 24–25 and 20–22°C) in standardized culture settings. Under the coldest temperatures (typical of offshore upwelling areas), development mirrored that obtained under warmer temperatures until the early auricularia stage, where development was arrested for ~5 months in a manner consistent with quiescence. When temperature was increased again, larvae quickly resumed development and settled; no lasting negative impact was noted. The findings suggest that the larvae can use a form of dormancy to withstand suboptimal oceanic conditions for indefinite periods of time until successful delivery to appropriate inshore locations, the habitat were I. fuscus is exclusively found.","PeriodicalId":14482,"journal":{"name":"Invertebrate Reproduction & Development","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41506600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-02DOI: 10.1080/07924259.2021.2019843
Tiantian Chen, Yingzhu Diao, Ruihan Xu, Na Sheng, Fan Liu, Qiming Xie, Shiping Su, K. Ma, Xilei Li
ABSTRACT The sesquiterpenoid methyl farnesoate (MF), a crustacean equivalent of juvenile hormone (JH III), plays important roles in regulating many physiological processes in crustaceans, especially ovarian development and reproduction. Previous research indicates that degradation of MF in crustaceans is similar to JH III degradation, and involves specific carboxylesterases. Juvenile hormone esterase hydrolase (JHEH) is another important enzyme responsible for JH inactivation in insects. In this study, the full-length cDNA of EsJHEH-like was identified and characterized in Eriocheir sinensis. Sequence analysis showed that EsJHEH-like belongs to the microsomal epoxide hydrolase (mEH) family containing several typical motifs. Quantitative PCR results showed that EsJHEH-like was expressed primarily in the hepatopancreas. During ovarian development, EsJHEH-like mRNA expression in the hepatopancreas was elevated specifically in the early vitellogenic stage, prior to the remarkable rise in haemolymph MF titre reported in previous studies, but no significant changes in the ovary. In addition, EsJHEH-like expression in the hepatopancreas was notably greater than in the ovary at each stage except for previtellogenic oocytes. Furthermore, EsJHEH-like expression in the hepatopancreas was significantly induced in vitro and in vivo, but not in the ovary. Taken together, these results suggest that EsJHEH-like may potentially serve as an MF hydrolase involved in the degradation of MF.
{"title":"Cloning and expression analysis of juvenile hormone epoxide hydrolase-like (EsJHEH-like) from Eriocheir sinensis, and its potential roles in methyl farnesoate metabolism","authors":"Tiantian Chen, Yingzhu Diao, Ruihan Xu, Na Sheng, Fan Liu, Qiming Xie, Shiping Su, K. Ma, Xilei Li","doi":"10.1080/07924259.2021.2019843","DOIUrl":"https://doi.org/10.1080/07924259.2021.2019843","url":null,"abstract":"ABSTRACT The sesquiterpenoid methyl farnesoate (MF), a crustacean equivalent of juvenile hormone (JH III), plays important roles in regulating many physiological processes in crustaceans, especially ovarian development and reproduction. Previous research indicates that degradation of MF in crustaceans is similar to JH III degradation, and involves specific carboxylesterases. Juvenile hormone esterase hydrolase (JHEH) is another important enzyme responsible for JH inactivation in insects. In this study, the full-length cDNA of EsJHEH-like was identified and characterized in Eriocheir sinensis. Sequence analysis showed that EsJHEH-like belongs to the microsomal epoxide hydrolase (mEH) family containing several typical motifs. Quantitative PCR results showed that EsJHEH-like was expressed primarily in the hepatopancreas. During ovarian development, EsJHEH-like mRNA expression in the hepatopancreas was elevated specifically in the early vitellogenic stage, prior to the remarkable rise in haemolymph MF titre reported in previous studies, but no significant changes in the ovary. In addition, EsJHEH-like expression in the hepatopancreas was notably greater than in the ovary at each stage except for previtellogenic oocytes. Furthermore, EsJHEH-like expression in the hepatopancreas was significantly induced in vitro and in vivo, but not in the ovary. Taken together, these results suggest that EsJHEH-like may potentially serve as an MF hydrolase involved in the degradation of MF.","PeriodicalId":14482,"journal":{"name":"Invertebrate Reproduction & Development","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49149845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-02DOI: 10.1080/07924259.2022.2033331
K. Mezali, Zineb Lebouazda, Farah Slimane-Tamacha, Dina Lila Soualili
ABSTRACT The aim of this work is to study the biometric and reproductive characteristics of a population of Holothuria poli from the west Algerian coast. The study was carried out on 256 individuals (33.25 ± 12.38 g wet weight and 196.44 ± 37.81 mm relaxed length) collected from Oran Bay between October 2016 and September 2017. All individuals showed negative allometric growth (b = 1.586) and the population had a sex ratio of 1: 1 (male: female). Sexual maturity occurred at a relaxed length of 137 mm and at gutted body weight of 18 g. Macroscopic examination of the gonadal tubules revealed four sexual maturity stages (growth, maturity, spawning and post-spawning) and a reproductive pattern characterized by a single summer spawning period and autumnal sexual rest. The obtained data may constitute a database for the management of natural stocks of this sea cucumber species in Algerian waters.
{"title":"Biometry, size structure and reproductive cycle of the sanded sea cucumbers Holothuria poli (Echinodermata, Holothuriidae) from the west Algerian coast","authors":"K. Mezali, Zineb Lebouazda, Farah Slimane-Tamacha, Dina Lila Soualili","doi":"10.1080/07924259.2022.2033331","DOIUrl":"https://doi.org/10.1080/07924259.2022.2033331","url":null,"abstract":"ABSTRACT The aim of this work is to study the biometric and reproductive characteristics of a population of Holothuria poli from the west Algerian coast. The study was carried out on 256 individuals (33.25 ± 12.38 g wet weight and 196.44 ± 37.81 mm relaxed length) collected from Oran Bay between October 2016 and September 2017. All individuals showed negative allometric growth (b = 1.586) and the population had a sex ratio of 1: 1 (male: female). Sexual maturity occurred at a relaxed length of 137 mm and at gutted body weight of 18 g. Macroscopic examination of the gonadal tubules revealed four sexual maturity stages (growth, maturity, spawning and post-spawning) and a reproductive pattern characterized by a single summer spawning period and autumnal sexual rest. The obtained data may constitute a database for the management of natural stocks of this sea cucumber species in Algerian waters.","PeriodicalId":14482,"journal":{"name":"Invertebrate Reproduction & Development","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44986026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACT Hormone-sensitive lipase (HSL) is the rate-limiting enzyme of lipolysis, which has a certain effect on the reproductive development of parthenogens. To study the effect of HSL on gonad development in Whitmania pigra, a synchronous hermaphrodite, the Whitmania pigra HSL gene was cloned. The cloned Wpi-hsl has a total length of 2492 bp, and the ORF encodes a protein of 787 aa. It is predicted that the Wpi-HSL protein has 98 phosphorylation modification sites on serine, threonine and tyrosine residues. Domain prediction and multiple sequence comparison revealed that Wpi-HSL contains 3 HSL conserved domains, which are closely related to Helobdella robusta, and the amino acid sequence similarity and sequence identity are 49% and 39%, respectively. Fluorescence quantitative PCR results showed that in the mature period, the expression of Wpi-hsl in the male gonads was significantly higher than that in the female gonads. Wpi-hsl may play an important role in the development of W. pigra, especially the development of male gonads. The results of our study provide references for not only further research on leech gonadal development but also the study of reproductive development mechanisms.
{"title":"Cloning and expression analysis of the HSL gene in Whitmania pigra (Annelida: Hirudinea)","authors":"Li-Yuan Guo, Q. Guo, Hongzhuan Shi, Feng Yang, Yixiu Miao","doi":"10.1080/07924259.2022.2027289","DOIUrl":"https://doi.org/10.1080/07924259.2022.2027289","url":null,"abstract":"ABSTRACT Hormone-sensitive lipase (HSL) is the rate-limiting enzyme of lipolysis, which has a certain effect on the reproductive development of parthenogens. To study the effect of HSL on gonad development in Whitmania pigra, a synchronous hermaphrodite, the Whitmania pigra HSL gene was cloned. The cloned Wpi-hsl has a total length of 2492 bp, and the ORF encodes a protein of 787 aa. It is predicted that the Wpi-HSL protein has 98 phosphorylation modification sites on serine, threonine and tyrosine residues. Domain prediction and multiple sequence comparison revealed that Wpi-HSL contains 3 HSL conserved domains, which are closely related to Helobdella robusta, and the amino acid sequence similarity and sequence identity are 49% and 39%, respectively. Fluorescence quantitative PCR results showed that in the mature period, the expression of Wpi-hsl in the male gonads was significantly higher than that in the female gonads. Wpi-hsl may play an important role in the development of W. pigra, especially the development of male gonads. The results of our study provide references for not only further research on leech gonadal development but also the study of reproductive development mechanisms.","PeriodicalId":14482,"journal":{"name":"Invertebrate Reproduction & Development","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43327702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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