R. Xing, J. J. Chen, M. Chen, J. Lian, L. Li, Xiaoping Zhou, R. Liu, Y. Xie, W. Huang, H. Zhao, Y. Zeng
Background: Tumor radioresistance leads to a reduction in the efficiency of radiation therapy. It is very important to explore the cellular mechanisms leading to radioresistance and to find potential therapeutic targets, which might improve the efficacy of radiation therapy. This study was to investigate the role of ataxia-telangiectasia mutated (ATM) and murine double minute X (MDMX) in radioresistance in non-small cell lung cancer A549 cells and their corresponding mechanisms of action. Materials and Methods: Non-small cell lung cancer A549 cells were irradiated with X-rays in the presence or absence of ATM inhibitor. Cell survival, cell apoptosis, cell proliferation, mRNA of ATM and MDMX, and protein expression of ATM, MDMX, γ-H2AX, Caspase3, and Beclin1 were measured. Results: After the inhibitor (KU60019) treatment combined with X irradiation, the A549 cells showed a significant decrease in colony formations compared to the group received irradiation alone. The MDMX knockdown A549 cells showed a significant increase in colony formations compared to the control group. ATM downregulated the expression of MDMX after irradiation treatment in A549 cells. Irradiation led to a significant increase in γ-H2AX expression, but MDMX knockdown decreased the γ-H2AX expression after irradiation. The change of Caspase3 expression was the same as γ-H2AX. Irradiation led to a significant increase of Beclin1 expression and MDMX knockdown increased the Beclin1 expression after irradiation. Conclusion: This study indicated that ATM induced radioresistance through downregulating the expression of MDMX, which was at least partly associated with the activation of autophagy and the decrease of DNA damage in A549 cells.
{"title":"ATM induces radioresistance of non-small cell lung cancer A549 cells by downregulation of MDMX","authors":"R. Xing, J. J. Chen, M. Chen, J. Lian, L. Li, Xiaoping Zhou, R. Liu, Y. Xie, W. Huang, H. Zhao, Y. Zeng","doi":"10.52547/IJRR.18.4.835","DOIUrl":"https://doi.org/10.52547/IJRR.18.4.835","url":null,"abstract":"Background: Tumor radioresistance leads to a reduction in the efficiency of radiation therapy. It is very important to explore the cellular mechanisms leading to radioresistance and to find potential therapeutic targets, which might improve the efficacy of radiation therapy. This study was to investigate the role of ataxia-telangiectasia mutated (ATM) and murine double minute X (MDMX) in radioresistance in non-small cell lung cancer A549 cells and their corresponding mechanisms of action. Materials and Methods: Non-small cell lung cancer A549 cells were irradiated with X-rays in the presence or absence of ATM inhibitor. Cell survival, cell apoptosis, cell proliferation, mRNA of ATM and MDMX, and protein expression of ATM, MDMX, γ-H2AX, Caspase3, and Beclin1 were measured. Results: After the inhibitor (KU60019) treatment combined with X irradiation, the A549 cells showed a significant decrease in colony formations compared to the group received irradiation alone. The MDMX knockdown A549 cells showed a significant increase in colony formations compared to the control group. ATM downregulated the expression of MDMX after irradiation treatment in A549 cells. Irradiation led to a significant increase in γ-H2AX expression, but MDMX knockdown decreased the γ-H2AX expression after irradiation. The change of Caspase3 expression was the same as γ-H2AX. Irradiation led to a significant increase of Beclin1 expression and MDMX knockdown increased the Beclin1 expression after irradiation. Conclusion: This study indicated that ATM induced radioresistance through downregulating the expression of MDMX, which was at least partly associated with the activation of autophagy and the decrease of DNA damage in A549 cells.","PeriodicalId":14498,"journal":{"name":"Iranian Journal of Radiation Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46976131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Z. A. Ganjeh, M. Eslami-Kalantari, M. E. Loushab, A. Mowlavi
Background: The total yields of direct Single-Strand Breaks (SSBs) and Double -Strand Breaks (DSBs) in proton energies varying from 0.1 to 40 MeV were calculated. While other studies in this field have not used protons with energy less than 0.5 MeV, our results show interesting and complicated behavior of these protons. Materials and Methods: The simulation has been done using the Geant4-DNA toolkit. An atomic model of DNA geometry was simulated. Simulations were performed with a source in the Z-axis direction at the cell nucleus entrance with protons at energies of 0.1-1 MeV in 0.1 MeV steps, 5 MeV, and 10-40 MeV in 10 MeV steps. Results: The calculated SSB yields decreased from 60.08 (GbpGy) for 0.1 MeV proton energy to 49.52 (GbpGy) −1 for 0.5 MeV proton energy, and then it increased to 54.35 (GbpGy) in 40 MeV. The DSB yields decreased from 4.32 (GbpGy) for 0.1 MeV proton energy to 1.03 (GbpGy) for 40-MeV protons. The DSB yields for energies less than 0.5 MeV was about 56%, and for the other energy levels, it was 44%. As for SSB yields, 35% of the breaks arose from protons with an energy of fewer than 0.5 MeV and 65% from higher energies. Conclusion: It was found that the proton ranges with an energy less than 0.5 MeV are smaller than the cell size (10 μm), and 100% of the energy is deposited in the cell region. Then protons with these energies are the best choice to increase the number of
{"title":"Investigation of the direct DNA damages irradiated by protons of different energies using geant4-DNA toolkit","authors":"Z. A. Ganjeh, M. Eslami-Kalantari, M. E. Loushab, A. Mowlavi","doi":"10.52547/IJRR.18.4.809","DOIUrl":"https://doi.org/10.52547/IJRR.18.4.809","url":null,"abstract":"Background: The total yields of direct Single-Strand Breaks (SSBs) and Double -Strand Breaks (DSBs) in proton energies varying from 0.1 to 40 MeV were calculated. While other studies in this field have not used protons with energy less than 0.5 MeV, our results show interesting and complicated behavior of these protons. Materials and Methods: The simulation has been done using the Geant4-DNA toolkit. An atomic model of DNA geometry was simulated. Simulations were performed with a source in the Z-axis direction at the cell nucleus entrance with protons at energies of 0.1-1 MeV in 0.1 MeV steps, 5 MeV, and 10-40 MeV in 10 MeV steps. Results: The calculated SSB yields decreased from 60.08 (GbpGy) for 0.1 MeV proton energy to 49.52 (GbpGy) −1 for 0.5 MeV proton energy, and then it increased to 54.35 (GbpGy) in 40 MeV. The DSB yields decreased from 4.32 (GbpGy) for 0.1 MeV proton energy to 1.03 (GbpGy) for 40-MeV protons. The DSB yields for energies less than 0.5 MeV was about 56%, and for the other energy levels, it was 44%. As for SSB yields, 35% of the breaks arose from protons with an energy of fewer than 0.5 MeV and 65% from higher energies. Conclusion: It was found that the proton ranges with an energy less than 0.5 MeV are smaller than the cell size (10 μm), and 100% of the energy is deposited in the cell region. Then protons with these energies are the best choice to increase the number of","PeriodicalId":14498,"journal":{"name":"Iranian Journal of Radiation Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42706769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
U. Şeker, A. Aktaş, Y. Nergiz, S. B. Zincircioglu, Ketani
Background: The aim of this study was to investigate the protective potency of melatonin, amifostine (WR-2721), and N-acetylcysteine (NAC) when administered intraperitoneally (i.p.) 15 min before 10-Gy single-fraction radiotherapy. Materials and Methods: In this study, 35 female Sprague Dawley rats were divided into five groups of seven rats each. The rats in the control group did not receive any treatments. Rats in the radiotherapy, melatonin, amifostine, and NAC groups underwent abdomino-pelvic irradiation with 10-Gy single fraction gamma (γ) irradiation. Melatonin 50 mg/ kg, amifostine 200 mg/kg, and NAC 500 mg/kg were i.p. administered to the rats 15 min before irradiation. Animals were sacrificed 48 h after irradiation. Uterus samples were collected and, routine histopathological tissue processing was performed. Sections from tissue samples were stained with H&E and analyzed with the terminal deoxynucleotidyl transferase dUTP nick end labelling method (TUNEL assay). Results: Severe morphological degenerations and increases in the apoptotic index (AI) were observed in the radiotherapy group. Tissue protection and AI reduction were observed in the amifostine and NAC groups. Melatonin was more effective than amifostine and NAC. Morphological damage was almost completely repaired, and the AI of the melatonin group was quite similar to that of the control group. Conclusion: This experiment failed to determine a more successful administration technique of amifostine. The protective effects of amifostine and NAC were similar. Melatonin was more successful than these two drugs, and might be an alternative to amifostine when time, dose, or adverse effect constraints are encountered.
{"title":"Investigation of the protective effects of melatonin, amifostine (WR-2721), and N-acetylcysteine on radiotherapy-induced uterine tissue injury in rats","authors":"U. Şeker, A. Aktaş, Y. Nergiz, S. B. Zincircioglu, Ketani","doi":"10.52547/IJRR.18.4.791","DOIUrl":"https://doi.org/10.52547/IJRR.18.4.791","url":null,"abstract":"Background: The aim of this study was to investigate the protective potency of melatonin, amifostine (WR-2721), and N-acetylcysteine (NAC) when administered intraperitoneally (i.p.) 15 min before 10-Gy single-fraction radiotherapy. Materials and Methods: In this study, 35 female Sprague Dawley rats were divided into five groups of seven rats each. The rats in the control group did not receive any treatments. Rats in the radiotherapy, melatonin, amifostine, and NAC groups underwent abdomino-pelvic irradiation with 10-Gy single fraction gamma (γ) irradiation. Melatonin 50 mg/ kg, amifostine 200 mg/kg, and NAC 500 mg/kg were i.p. administered to the rats 15 min before irradiation. Animals were sacrificed 48 h after irradiation. Uterus samples were collected and, routine histopathological tissue processing was performed. Sections from tissue samples were stained with H&E and analyzed with the terminal deoxynucleotidyl transferase dUTP nick end labelling method (TUNEL assay). Results: Severe morphological degenerations and increases in the apoptotic index (AI) were observed in the radiotherapy group. Tissue protection and AI reduction were observed in the amifostine and NAC groups. Melatonin was more effective than amifostine and NAC. Morphological damage was almost completely repaired, and the AI of the melatonin group was quite similar to that of the control group. Conclusion: This experiment failed to determine a more successful administration technique of amifostine. The protective effects of amifostine and NAC were similar. Melatonin was more successful than these two drugs, and might be an alternative to amifostine when time, dose, or adverse effect constraints are encountered.","PeriodicalId":14498,"journal":{"name":"Iranian Journal of Radiation Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70687259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-10DOI: 10.18869/ACADPUB.IJRR.18.3.413
S.S.A. Jahanbani, A. Rezaeyan, H. Ghaffari, A. R. Babae, G. Haddadi, M. Moeni, A. Jomehzadeh, S. Moatamedi, S. H. Eftekhar-Vaghefi
Background: Radiation-induced lung injury is a common complication after esophageal, breast, etc. cancer radiotherapy. We aimed to evaluate the radioprotective effect of arbutin on acute radiation-induced lung damage in male rats. Materials and Methods: Fifty-two male Wistar rats were divided into 4 groups: (i) control group (n = 10), (ii) vehicle group (n = 10, received distilled water intraperitoneally (ip)), (iii) X-irradiation only (n=16, chest was irradiated to a single dose of 20 Gy x-rays) and (iv) arbutin + X-irradiation (n=16, 75 mg/kg of arbutin 2 hours before irradiation (ip), and then their chest was exposed to 20 Gy x-rays). For histopathological investigation, 8 animals of each group were sacrificed 8 weeks after treatment and the rest of them were sacrificed 3 months after treatment. Results: The histopathological analysis in 8 weeks after X-irradiation showed that there was a significant increase in inflammatory in X-irradiation only group compared to control group. The administration of arbutin 2 hours prior to X-irradiation significantly reduced inflammation and inflammatory factors such as macrophages, mast cell and neutrophil in arbutin + X-irradiation group compared to X-irradiation only group (P<0.05). The histopathologic investigation performed 3 months after lung irradiation indicated a significant reduction in fibrosis formation in arbutin + X-irradiation group compared to irradiation only group (P<0.05). Localized chest X-irradiation with 20 Gy caused histopathologic damage to the lungs for short -term. Conclusion: Arbutin has a great potential in reducing the histopathologic damage to lung tissue after thoracic irradiation.
{"title":"Investigation of the radioprotective effect of arbutin on radiation-induced lung injury in rats: a histopathological study","authors":"S.S.A. Jahanbani, A. Rezaeyan, H. Ghaffari, A. R. Babae, G. Haddadi, M. Moeni, A. Jomehzadeh, S. Moatamedi, S. H. Eftekhar-Vaghefi","doi":"10.18869/ACADPUB.IJRR.18.3.413","DOIUrl":"https://doi.org/10.18869/ACADPUB.IJRR.18.3.413","url":null,"abstract":"Background: Radiation-induced lung injury is a common complication after esophageal, breast, etc. cancer radiotherapy. We aimed to evaluate the radioprotective effect of arbutin on acute radiation-induced lung damage in male rats. Materials and Methods: Fifty-two male Wistar rats were divided into 4 groups: (i) control group (n = 10), (ii) vehicle group (n = 10, received distilled water intraperitoneally (ip)), (iii) X-irradiation only (n=16, chest was irradiated to a single dose of 20 Gy x-rays) and (iv) arbutin + X-irradiation (n=16, 75 mg/kg of arbutin 2 hours before irradiation (ip), and then their chest was exposed to 20 Gy x-rays). For histopathological investigation, 8 animals of each group were sacrificed 8 weeks after treatment and the rest of them were sacrificed 3 months after treatment. Results: The histopathological analysis in 8 weeks after X-irradiation showed that there was a significant increase in inflammatory in X-irradiation only group compared to control group. The administration of arbutin 2 hours prior to X-irradiation significantly reduced inflammation and inflammatory factors such as macrophages, mast cell and neutrophil in arbutin + X-irradiation group compared to X-irradiation only group (P<0.05). The histopathologic investigation performed 3 months after lung irradiation indicated a significant reduction in fibrosis formation in arbutin + X-irradiation group compared to irradiation only group (P<0.05). Localized chest X-irradiation with 20 Gy caused histopathologic damage to the lungs for short -term. Conclusion: Arbutin has a great potential in reducing the histopathologic damage to lung tissue after thoracic irradiation.","PeriodicalId":14498,"journal":{"name":"Iranian Journal of Radiation Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45363823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-10DOI: 10.18869/ACADPUB.IJRR.18.3.427
B. A. El-Badry, T. I. AL-Naggar, G. Khouqeer
Background: The existence of radioactive isotopes and toxic elements in water poses a potential threat to public health. Due to the high consumption of bottled water, the focus of this study is on measuring the concentration of radioactive isotopes (Rn and Ra) and toxic elements (Cr, Mn, Co, Ni, Cu, Zn, Cd, Pb) in different brands of bottled drinking water. Materials and Methods: Therefore, twenty-four samples of bottled water have been carefully selected from local markets in Saudi Arabia. Nuclear track detector of type CR-39 and Inductive Coupled Plasma-Mass Spectrometer were used to measure radioactivity concentrations and toxic elements, respectively. Results: It is observed that the activity concentration of Rn and Ra in all samples was lower than the recommended values set by different agencies such as WHO, and EPA. The annual effective dose for three age groups (infants, children, and adults) was calculated from the concentration of Rn. These measurements provide basic information for consumers who could be at risk of exposure through bottled water consumption. Conclusion: then all types of bottled drinking water are suitable and safe for daily population ingestion.
{"title":"Monitoring the levels of radon and toxic elements pollutants in bottled drinking water","authors":"B. A. El-Badry, T. I. AL-Naggar, G. Khouqeer","doi":"10.18869/ACADPUB.IJRR.18.3.427","DOIUrl":"https://doi.org/10.18869/ACADPUB.IJRR.18.3.427","url":null,"abstract":"Background: The existence of radioactive isotopes and toxic elements in water poses a potential threat to public health. Due to the high consumption of bottled water, the focus of this study is on measuring the concentration of radioactive isotopes (Rn and Ra) and toxic elements (Cr, Mn, Co, Ni, Cu, Zn, Cd, Pb) in different brands of bottled drinking water. Materials and Methods: Therefore, twenty-four samples of bottled water have been carefully selected from local markets in Saudi Arabia. Nuclear track detector of type CR-39 and Inductive Coupled Plasma-Mass Spectrometer were used to measure radioactivity concentrations and toxic elements, respectively. Results: It is observed that the activity concentration of Rn and Ra in all samples was lower than the recommended values set by different agencies such as WHO, and EPA. The annual effective dose for three age groups (infants, children, and adults) was calculated from the concentration of Rn. These measurements provide basic information for consumers who could be at risk of exposure through bottled water consumption. Conclusion: then all types of bottled drinking water are suitable and safe for daily population ingestion.","PeriodicalId":14498,"journal":{"name":"Iranian Journal of Radiation Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43058881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-10DOI: 10.18869/ACADPUB.IJRR.18.3.549
J. Chen, Yang Xiao, L. Wu, Feng-Shou Xiao, Q. Wang, J. Hong, P. Zhu
Background: To investigate effect of radiosensitization of [Ru(bpy)2(phen)] Cl2 complex on nasopharyngeal carcinoma cell line CNE1 and its mechanism. Materials and Methods: Nasopharyngeal carcinoma cell line CNE1 in vitro culture was divided into control group, light irradiation group (4 Gy, 6 MV photonic line), simple metal ruthenium complex treatment group (Ru group, 100 μmol/L [Ru(bpy)2(phen)]Cl2) and metal ruthenium complex combined with radiotherapy group (Combined radiotherapy group, cells were irradiated with 4 Gy and 6 MV photons at 2 days after 100 μmol/L [Ru(bpy)2(phen)]Cl2). Results: Transcriptional level of P53 gene in combined radiotherapy group was higher than that in the other groups (P<0.001). Inhibition rate of combined radiotherapy group was higher than that of Ru group and irradiation group (P<0.001). Apoptotic rate was the highest (P<0.05) in the combined radiotherapy group, and irradiation group was higher than Ru group and control group (P<0.05). Survival rate of Ru group was lower than that of control group under the same radiation dose (P<0.05), and the radiotherapy sensitization ratio was 1.227 (Dq ratio). Conclusion: [Ru(bpy)2 (phen)]Cl2 increases the sensitivity of nasopharyngeal carcinoma cell line CNE1 to X-ray, which may be related to increase of P53 gene expression.
{"title":"Radiosensitization of [Ru(bpy)2(phen)]Cl2 on nasopharyngeal carcinoma cells","authors":"J. Chen, Yang Xiao, L. Wu, Feng-Shou Xiao, Q. Wang, J. Hong, P. Zhu","doi":"10.18869/ACADPUB.IJRR.18.3.549","DOIUrl":"https://doi.org/10.18869/ACADPUB.IJRR.18.3.549","url":null,"abstract":"Background: To investigate effect of radiosensitization of [Ru(bpy)2(phen)] Cl2 complex on nasopharyngeal carcinoma cell line CNE1 and its mechanism. Materials and Methods: Nasopharyngeal carcinoma cell line CNE1 in vitro culture was divided into control group, light irradiation group (4 Gy, 6 MV photonic line), simple metal ruthenium complex treatment group (Ru group, 100 μmol/L [Ru(bpy)2(phen)]Cl2) and metal ruthenium complex combined with radiotherapy group (Combined radiotherapy group, cells were irradiated with 4 Gy and 6 MV photons at 2 days after 100 μmol/L [Ru(bpy)2(phen)]Cl2). Results: Transcriptional level of P53 gene in combined radiotherapy group was higher than that in the other groups (P<0.001). Inhibition rate of combined radiotherapy group was higher than that of Ru group and irradiation group (P<0.001). Apoptotic rate was the highest (P<0.05) in the combined radiotherapy group, and irradiation group was higher than Ru group and control group (P<0.05). Survival rate of Ru group was lower than that of control group under the same radiation dose (P<0.05), and the radiotherapy sensitization ratio was 1.227 (Dq ratio). Conclusion: [Ru(bpy)2 (phen)]Cl2 increases the sensitivity of nasopharyngeal carcinoma cell line CNE1 to X-ray, which may be related to increase of P53 gene expression.","PeriodicalId":14498,"journal":{"name":"Iranian Journal of Radiation Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49494959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-10DOI: 10.18869/ACADPUB.IJRR.18.3.557
K. Venkatesan, C. Raphael, K. Varghese, Paul G Gopu, S. Sivakumar, M. Boban, N. A. N. Raj, K. Senthilnathan, P. Babu
Background: To study and analyze the variations in delivered doses to rectum and bladder of carcinoma prostate patients by using kilo Voltage (kV) ‘Cone Beam Computed Tomography’ (CBCT) images. Materials and Methods: 2-Dimensional kilo Voltage (2D kV) Imaging and CBCT were done for seven prostate cancer patients. The deviations among their shifts were correlated and the volumetric changes of the rectum and bladder were analyzed. Rectum and bladder contours were redrawn on every boost fractions and dose calculation were performed on CBCT images to study the effect on dose volume histograms. Results: A correlation coefficient for set-up variations was within 0.78 for all directions between CBCT soft tissue matching and kV bone match. The mean deviation of bladder and rectum volume over the boost fractions was -12% to +9% (SD 31cc to 70cc) and -10.2% to+12% (SD 3.1cc to14.9cc), respectively. Bladder mean dose variation was <1.5Gy for all three positions whereas it was <3.65Gy for rectum. D1% dose deviation from reference plan for bladder was 1.1Gy (CBCT matched position), 1.4Gy (kV matched position), and 1.7Gy (no correction), and for rectum, the deviations were 1.2Gy, 2.2Gy, 3.6Gy, respectively. No significant deviation was found statistically significant at the low dose region. Conclusion: It is possible to achieve good dose delivery and conformity in target (prostate) with CBCT image guidance rather than kV bone match, but dose contribution to the rectum is dependent on the patient’s anatomy, bladder filling, and rectum filling, pertaining to the day of examination.
{"title":"Volume and dosimetric analysis of rectum and bladder for prostate carcinoma patients by using kilo voltage cone beam computed tomography (CBCT)","authors":"K. Venkatesan, C. Raphael, K. Varghese, Paul G Gopu, S. Sivakumar, M. Boban, N. A. N. Raj, K. Senthilnathan, P. Babu","doi":"10.18869/ACADPUB.IJRR.18.3.557","DOIUrl":"https://doi.org/10.18869/ACADPUB.IJRR.18.3.557","url":null,"abstract":"Background: To study and analyze the variations in delivered doses to rectum and bladder of carcinoma prostate patients by using kilo Voltage (kV) ‘Cone Beam Computed Tomography’ (CBCT) images. Materials and Methods: 2-Dimensional kilo Voltage (2D kV) Imaging and CBCT were done for seven prostate cancer patients. The deviations among their shifts were correlated and the volumetric changes of the rectum and bladder were analyzed. Rectum and bladder contours were redrawn on every boost fractions and dose calculation were performed on CBCT images to study the effect on dose volume histograms. Results: A correlation coefficient for set-up variations was within 0.78 for all directions between CBCT soft tissue matching and kV bone match. The mean deviation of bladder and rectum volume over the boost fractions was -12% to +9% (SD 31cc to 70cc) and -10.2% to+12% (SD 3.1cc to14.9cc), respectively. Bladder mean dose variation was <1.5Gy for all three positions whereas it was <3.65Gy for rectum. D1% dose deviation from reference plan for bladder was 1.1Gy (CBCT matched position), 1.4Gy (kV matched position), and 1.7Gy (no correction), and for rectum, the deviations were 1.2Gy, 2.2Gy, 3.6Gy, respectively. No significant deviation was found statistically significant at the low dose region. Conclusion: It is possible to achieve good dose delivery and conformity in target (prostate) with CBCT image guidance rather than kV bone match, but dose contribution to the rectum is dependent on the patient’s anatomy, bladder filling, and rectum filling, pertaining to the day of examination.","PeriodicalId":14498,"journal":{"name":"Iranian Journal of Radiation Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46111375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-10DOI: 10.18869/ACADPUB.IJRR.18.3.397
S. Arafin, A. El-Taher, A. Hoque, M. Hoque, J. Ferdous, M. Abedi
Background: The present work was conducted on some soil samples collected from Shamnagar upazila of Satkhira district of south-west part of world largest mangrove forest Sundarban Rezion, Bangladesh which were affected by natural disaster cyclone Aila. Materials and Methods: The soil samples were analyzed to determine terrestrial γ-ray activity using HPGe γ-ray spectrometry. The measurements conducted in the current study showed that primordial radionuclides namely the U and Th and K are contained in all the soil samples. Results and Discussion: The obtained results of the average activity concentrations of U, Th and K were found to be within the range of the world average of 17-60, 11-64, 140-850 Bq.kg reported by UNSCEAR. The radiation dose is below the permissible limit of 1mSvy recommended by IAEA (2007). No peak of Cs from the decay activity was found in the spectrum in the present work. Conclusion: The present work will be useful in providing environmental monitoring data base of those particular areas.
{"title":"Natural gamma radiation level detection in agriculture soil after Aila disaster and comparison with deep soil gamma activity in a specific area of Sundarban region, Satkhira, Banglsdesh","authors":"S. Arafin, A. El-Taher, A. Hoque, M. Hoque, J. Ferdous, M. Abedi","doi":"10.18869/ACADPUB.IJRR.18.3.397","DOIUrl":"https://doi.org/10.18869/ACADPUB.IJRR.18.3.397","url":null,"abstract":"Background: The present work was conducted on some soil samples collected from Shamnagar upazila of Satkhira district of south-west part of world largest mangrove forest Sundarban Rezion, Bangladesh which were affected by natural disaster cyclone Aila. Materials and Methods: The soil samples were analyzed to determine terrestrial γ-ray activity using HPGe γ-ray spectrometry. The measurements conducted in the current study showed that primordial radionuclides namely the U and Th and K are contained in all the soil samples. Results and Discussion: The obtained results of the average activity concentrations of U, Th and K were found to be within the range of the world average of 17-60, 11-64, 140-850 Bq.kg reported by UNSCEAR. The radiation dose is below the permissible limit of 1mSvy recommended by IAEA (2007). No peak of Cs from the decay activity was found in the spectrum in the present work. Conclusion: The present work will be useful in providing environmental monitoring data base of those particular areas.","PeriodicalId":14498,"journal":{"name":"Iranian Journal of Radiation Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49197369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-10DOI: 10.18869/ACADPUB.IJRR.18.3.405
O. Ozmen, O. Kavrik
Background: The aim of this study was to investigate hepatic lesions induced by the 2.45 GHz electromagnetic radiation (EMR) of Wi-Fi and the protective effects of Vitamin C (Vit C) in rats. Materials and Methods: Eighteen SpragueDawley female rats were randomly distributed into three groups, each containing six rats. The groups were Control, EMR (EMR, 1 h/day for 30 days) and EMR + Vit C (EMR, 1 h/day for 30 days and Vit C, 250 mg/kg/daily). At the end of the study, blood and liver tissue samples were collected for laboratory examinations. Results: Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were increased in the EMR group compared with the control group. Liver histology of rats in the EMR group revealed severe hyperemia, slight degeneration, and lipidosis. Caspase-3, hypoxiainducible factor-1 (HIF-1), and HIF-2 immunoexpression was increased, and Sirtuin-1 (SIRT-1) expression was markedly decreased in hepatocytes in the EMR group. Vit C treatment ameliorated both biochemical and pathological findings. Conclusion: The results of this study showed that 2.45 GHz (Wi-Fi) EMR can cause liver damage in rats, but Vit C has an ameliorative effect.
{"title":"Ameliorative effects of vitamin C against hepatic pathology related to Wi-Fi (2.45 GHz electromagnetic radiation) in rats","authors":"O. Ozmen, O. Kavrik","doi":"10.18869/ACADPUB.IJRR.18.3.405","DOIUrl":"https://doi.org/10.18869/ACADPUB.IJRR.18.3.405","url":null,"abstract":"Background: The aim of this study was to investigate hepatic lesions induced by the 2.45 GHz electromagnetic radiation (EMR) of Wi-Fi and the protective effects of Vitamin C (Vit C) in rats. Materials and Methods: Eighteen SpragueDawley female rats were randomly distributed into three groups, each containing six rats. The groups were Control, EMR (EMR, 1 h/day for 30 days) and EMR + Vit C (EMR, 1 h/day for 30 days and Vit C, 250 mg/kg/daily). At the end of the study, blood and liver tissue samples were collected for laboratory examinations. Results: Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were increased in the EMR group compared with the control group. Liver histology of rats in the EMR group revealed severe hyperemia, slight degeneration, and lipidosis. Caspase-3, hypoxiainducible factor-1 (HIF-1), and HIF-2 immunoexpression was increased, and Sirtuin-1 (SIRT-1) expression was markedly decreased in hepatocytes in the EMR group. Vit C treatment ameliorated both biochemical and pathological findings. Conclusion: The results of this study showed that 2.45 GHz (Wi-Fi) EMR can cause liver damage in rats, but Vit C has an ameliorative effect.","PeriodicalId":14498,"journal":{"name":"Iranian Journal of Radiation Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44987850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-10DOI: 10.18869/ACADPUB.IJRR.18.3.587
A. Salehi, K. Ebrahimpour, F. Forouharmajd, M. Zarean
{"title":"The relationship between collective effective doses of radiation and urinary concentration of 8-Dihydroxy-2'-Deoxyguanosine among radiography staff","authors":"A. Salehi, K. Ebrahimpour, F. Forouharmajd, M. Zarean","doi":"10.18869/ACADPUB.IJRR.18.3.587","DOIUrl":"https://doi.org/10.18869/ACADPUB.IJRR.18.3.587","url":null,"abstract":"","PeriodicalId":14498,"journal":{"name":"Iranian Journal of Radiation Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45312307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：