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Comprehensive review of salmonellosis: current status of the disease and future perspectives. 沙门氏菌病综合综述:疾病现状和未来展望。
IF 1.8 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-10-01 eCollection Date: 2024-11-12 DOI: 10.4081/ijfs.2024.12904
Ohoud S Alhumaidan

Salmonellosis is a significant public health concern in Saudi Arabia, with various serovars of Salmonella causing outbreaks and infections. The disease's clinical presentation includes common symptoms such as diarrhea, fever, and abdominal cramps, with potential complications in severe cases. Diagnosing salmonellosis in Saudi Arabia involves a combination of traditional laboratory methods and molecular techniques to ensure accurate identification and treatment. Preventive measures and control strategies, including vaccination campaigns, food safety protocols, and public health education, have been implemented to mitigate the spread of salmonellosis. Challenges such as antimicrobial resistance, limited healthcare resources in rural areas, and underreporting of cases persist and impact the effective management of the disease. Recommendations for improving salmonellosis prevention and management include enhancing surveillance, implementing public health education campaigns, strengthening food safety regulations, promoting antimicrobial stewardship, investing in research, improving healthcare infrastructure, and fostering collaboration and coordination between sectors. Implementing these recommendations can help Saudi Arabia effectively address the challenges posed by salmonellosis and reduce the burden of the disease on public health.

沙门氏菌病是沙特阿拉伯的一个重大公共卫生问题,各种血清型沙门氏菌引起暴发和感染。该病的临床表现包括常见症状,如腹泻、发烧和腹部痉挛,严重者可能出现并发症。在沙特阿拉伯诊断沙门氏菌病需要结合传统实验室方法和分子技术,以确保准确识别和治疗。已实施预防措施和控制战略,包括疫苗接种运动、食品安全规程和公共卫生教育,以减轻沙门氏菌病的传播。诸如抗菌素耐药性、农村地区卫生保健资源有限以及病例少报等挑战持续存在,并影响对该病的有效管理。关于改善沙门氏菌病预防和管理的建议包括加强监测、开展公共卫生教育运动、加强食品安全法规、促进抗微生物药物管理、投资研究、改善卫生保健基础设施以及促进部门之间的合作与协调。实施这些建议可帮助沙特阿拉伯有效应对沙门氏菌病带来的挑战,并减轻该疾病对公共卫生造成的负担。
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引用次数: 0
Speck: a traditional culinary specialty from the Italian Alps. A microbiological, molecular and chemical evaluation. Speck:一种来自意大利阿尔卑斯山的传统美食。微生物、分子和化学评价。
IF 1.8 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-09-25 eCollection Date: 2024-11-12 DOI: 10.4081/ijfs.2024.12630
Stefano Colorio, Francesca Precazzini, Martina Stella, Michela Rabini, Gloria Paolazzi, Evelin Elke Oberkalmsteiner, Mara Borghi, Astrid Bettini, Alexander Tavella

South Tyrol is the northernmost Italian province; its history, geography, and closeness to other European countries, such as Austria and Switzerland, have influenced both culture and food production. Among the South Tyrolean culinary specialties, a type of dry-cured ham called Südtiroler Speck/Speck Alto Adige (Speck) plays a relevant role and has gained increasing significance both on a national and international level over the last 2 decades. Despite it being a common culinary product in Italian and international markets, there is not much published data regarding the microbiological and chemical features of Speck. This study describes the analytical results obtained during a period of 7 years, during which the main pathogens and contaminants were considered.

南蒂罗尔是意大利最北的省份;它的历史、地理位置以及与奥地利和瑞士等其他欧洲国家的密切关系,对文化和食品生产都产生了影响。在南蒂罗尔的烹饪特色菜中,一种被称为 dtiroler Speck/Speck Alto Adige (Speck)的干腌火腿发挥着重要作用,在过去20年里,它在国内和国际上都越来越重要。尽管它在意大利和国际市场上是一种常见的烹饪产品,但关于Speck的微生物和化学特征的公开数据并不多。本研究描述了在7年期间获得的分析结果,在此期间主要病原体和污染物被考虑。
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引用次数: 0
Insect-based products commercialized online: a snapshot of lipid oxidation and mineral content. 网上商业化的昆虫产品:脂质氧化和矿物质含量的快照。
IF 1.8 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-08-06 eCollection Date: 2024-11-12 DOI: 10.4081/ijfs.2024.11600
Stefania Balzan, Luca Fasolato, Federico Fontana, Sarah Currò, Enrico Novelli

This research aims to monitor the conservation status of the lipid and mineral contents of four shelf-stable insect-based products (yellow mealworm, house cricket, mole cricket, and silkworm) marketed online. A total of 32 single-species packs were purchased from various online commercial suppliers. Moisture, lipids, fatty acids, titratable acidity, mineral elements, and primary and secondary lipid oxidation products were determined. Statistical multivariate approaches were applied to investigate the contribution of each chemical variable to the characterization of edible insects. Titratable acidity (up to 37.3 g oleic acid per 100 g of crickets), as well as primary and secondary lipid oxidation products, showed great variability within and between species. The study revealed a significant occurrence of rancidity (45.5% of the samples exceeded the peroxide limit of 10 mEqO2/kg; 100% of the samples exceeded the indication of 1 mg/kg malondialdehyde), whereas the heavy metal content indicated a relatively safe condition, suggesting the absence of potential risks to human health. Both the chemical and the elemental properties could be regarded as potential characteristics suitable for authenticating this food matrix. This study contributes to the description of several chemical features in commercialized processed insect-based products, aiming to highlight possible safety concerns and identify those unfit for human consumption.

本研究旨在监测网上销售的四种货架稳定的昆虫产品(黄粉虫、家蟋蟀、鼹鼠蟋蟀和蚕)的脂质和矿物质含量的保存状况。从不同的网上商业供应商共购买了32个单物种包。测定了水分、脂质、脂肪酸、可滴定酸度、矿物元素和初级和次级脂质氧化产物。采用多元统计方法研究了各化学变量对食用昆虫特性的贡献。可滴定的酸度(每100克蟋蟀高达37.3克油酸),以及初级和次级脂质氧化产物,在物种内部和物种之间表现出很大的差异。研究发现,严重的酸败发生(45.5%的样品超过了10 mEqO2/kg的过氧化限;100%的样品超过了1 mg/kg(丙二醛)的指示值,而重金属含量则显示出相对安全的状况,表明对人体健康不存在潜在风险。化学性质和元素性质均可作为鉴定该食品基质的潜在特征。这项研究有助于描述商业化加工昆虫产品的几种化学特征,旨在强调可能存在的安全问题,并确定哪些不适合人类食用。
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引用次数: 0
Sardinian fermented sausage traditional production process: a preliminary survey in eight establishments. 撒丁岛发酵香肠的传统生产工艺:八家企业的初步调查。
IF 1.8 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-08-02 eCollection Date: 2024-11-12 DOI: 10.4081/ijfs.2024.12203
Giuliana Siddi, Francesca Piras, Maria Pina Meloni, Vincenzo Spanu, Nadia Carta, Mario Cuccu, Carlo Spanu, Riccardo Di Salvo, Carlo Piga, Enrico Pietro Luigi De Santis, Christian Scarano

This study aimed to conduct a preliminary investigation in eight Sardinian fermented sausage (SFS) production plants to acquire knowledge about the differences in the applied technological process and their influence on the safety and sensory characteristics of the finished product. Two audits were conducted in each plant to evaluate structural characteristics and process technologies; 72 samples of SFS at the end of seasoning and 48 environmental samples were analyzed. Listeria monocytogenes, Listeria spp., Salmonella spp., and Yersinia enterocolitica were investigated, and chemical-physical analyses were also performed. A panel of consumers was subjected to the Check All That Apply test and acceptability test to determine the qualities perceived by consumers and assess the product acceptance rating. A water activity value of >0.941, permissive for the growth of L. monocytogenes, was detected in SFS produced by one producing plant; L. monocytogenes, Salmonella spp., and Y. enterocolitica were detected in 2.8% of SFS samples, and Listeria spp. in 20.8% of samples. Environmental samples tested positive for 45.8% of Listeria spp. and 16.7% of L. monocytogenes. Correct drying and ripening steps, applied for at least 20 days, are critical for the development of hurdles required to guarantee the safety of fermented sausages. The application of proper hygiene and cleaning procedures is required to reduce environmental contamination. Sensory analysis results show how the production processes applied determine the marketing of sensorially different products. The ideal profile suggested by consumers confirms that the attributes that allow for improved liking are "moderate spicing", "moderate spiciness", "seasoned product", and "artisanal character".

本研究旨在对8家撒丁岛发酵香肠(SFS)生产厂进行初步调查,以了解应用工艺流程的差异及其对成品安全性和感官特性的影响。在每个工厂进行了两次审核,以评估结构特性和工艺技术;对调味末72份SFS样品和48份环境样品进行了分析。调查了单核增生李斯特菌、李斯特菌、沙门氏菌和小肠结肠炎耶尔森菌,并进行了化学-物理分析。由一组消费者组成的小组接受了“Check All That Apply”测试和可接受性测试,以确定消费者所感知的质量,并评估产品的可接受程度。其中一株生产的SFS的水分活度为>0.941,有利于单核增生乳杆菌的生长;单细胞增生乳杆菌、沙门氏菌和小肠结肠炎耶夫氏菌分别在2.8%和20.8%的样品中检出。环境样品检测李斯特菌45.8%阳性,单核增生李斯特菌16.7%阳性。正确的干燥和成熟步骤至少需要20天,这对于保证发酵香肠安全所需的障碍的发展至关重要。应用适当的卫生和清洁程序是必要的,以减少环境污染。感官分析结果显示了生产过程的应用如何决定了不同感官产品的销售。消费者建议的理想形象证实,允许提高喜欢的属性是“适度的香料”,“适度的辛辣”,“成熟的产品”和“手工特征”。
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引用次数: 0
Behind the scenes of taste: an exploratory study of non-compliance in Italian artisanal bakery and pastry laboratories. 口味的幕后:意大利手工烘焙和糕点实验室不合规的探索性研究。
IF 1.8 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-08-02 eCollection Date: 2024-11-12 DOI: 10.4081/ijfs.2024.12235
Sarah Currò, Stefania Balzan, Valentina Saccarola, Federico Fontana, Enrico Novelli, Luca Fasolato

This study aimed to identify critical issues in artisanal bakery and pastry production in Italy that could improve food safety and quality. Fifteen voluntary Italian companies underwent on-site inspections and interviews from 2018 to 2021. The inspection concerned the production site characteristics, processing flows, materials, and personnel to pinpoint potential product contamination and record objective data collection through a 126-question demerit scoring system. The examined areas encompassed various aspects, such as the point of sale, management of raw materials and packaging, hygiene practices, finished product quality, sanitization procedures, external spaces, personnel, and other factors impacting food safety and hygiene. Additionally, assessments of the microbiological air quality were carried out. Two cohorts (six and nine companies in 2018-2019 and in 2020-2021, respectively) revealed critical issues in warehouse/packaging (70% non-compliance), finished product/cooling (50% non-compliance), and people/products flow (38% non-compliance). Site visits identified pest management and raw material handling challenges. Significant airborne mold and yeast contamination (30-50 colony-forming units/plate/h) were observed in areas near processing sites or with air turbulence. This study facilitated constructive discussions and proposed solutions with the participating companies.

这项研究旨在找出意大利手工烘焙和糕点生产中的关键问题,从而提高食品安全和质量。15 家自愿参与的意大利公司在 2018 年至 2021 年期间接受了现场检查和访谈。检查内容涉及生产现场特征、加工流程、材料和人员,以找出潜在的产品污染,并通过 126 个问题的扣分系统记录客观的数据收集。检查范围包括销售点、原材料和包装管理、卫生规范、成品质量、消毒程序、外部空间、人员及其他影响食品安全卫生的因素等各个方面。此外,还对微生物空气质量进行了评估。两批企业(2018-2019 年和 2020-2021 年分别有 6 家和 9 家企业)发现了仓库/包装(70% 不合规)、成品/冷却(50% 不合规)和人员/产品流动(38% 不合规)方面的关键问题。实地考察发现了虫害管理和原材料处理方面的挑战。在加工现场附近或空气湍流的区域,观察到了严重的空气霉菌和酵母菌污染(30-50 菌落数/盘/小时)。这项研究有助于与参与公司进行建设性讨论并提出解决方案。
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引用次数: 0
Risk analysis of critical control points of Staphylococcus aureus in layer farms and chicken egg distributors. 蛋场及鸡蛋经销商金黄色葡萄球菌关键控制点风险分析。
IF 1.8 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-06-26 eCollection Date: 2024-11-12 DOI: 10.4081/ijfs.2024.12409
Aminah Hajah Thaha, Ratmawati Malaka, Wahniyati Hatta, Fatma Maruddin

Microbiological criteria play a role in verifying the critical control points (CCP), which become part of the hazard analysis, and the CCP system that guarantees quality, considering possible danger points or stages in the food production chain. Studies about Staphylococcus aureus in chicken eggs more extensively discuss the path, source, and level of prevalence of contamination at the final distributor or consumer. Therefore, this study investigates CCP contamination of S. aureus in chicken eggs and their potential consumption, which could endanger human health from the layer farm until the final distributor. This study is critical in health, public health, and veterinary medicine for preventing and controlling consumers' security. This study done for CCP on the chain distribution of chicken eggs starts with preparing production and distribution process flow diagrams for livestock, agents/wholesalers, and retailers. Confirmation of operational production in the field/location study is based on the flow chart that has been arranged, identifying all potential dangers associated with each stage and analyzing potential risks considering every action for controlling identified hazards. The next step involves pinpointing the CCP to counteract the identified threat. An application tree decision defines the CCP, and the logical and final approach is the determination limit critical to the CCP. Analysis results in the determination of CCP contamination of S. aureus, indicating that chicken eggs, personal hands (farm workers, wholesalers, and retailers), shelf eggs, and feces are CCP on farms.

微生物标准在验证关键控制点(CCP)方面发挥作用,关键控制点(CCP)成为危害分析的一部分,CCP系统保证质量,考虑到食品生产链中可能存在的危险点或阶段。关于鸡蛋中金黄色葡萄球菌的研究更广泛地讨论了最终分销商或消费者污染的途径、来源和流行程度。因此,本研究调查了鸡蛋中金黄色葡萄球菌的CCP污染及其潜在消费,从蛋场到最终经销商可能危害人类健康。该研究对卫生、公共卫生和兽医学预防和控制消费者安全具有重要意义。这项为CCP所做的关于鸡蛋连锁分销的研究从准备牲畜、代理商/批发商和零售商的生产和分销流程流程图开始。在现场/位置研究中,作业生产的确认是基于已安排的流程图,识别与每个阶段相关的所有潜在危险,并考虑控制已识别危险的每项行动来分析潜在风险。下一步包括精确定位CCP以抵消已识别的威胁。应用程序树决策定义CCP,逻辑和最终的方法是对CCP至关重要的决定限制。金黄色葡萄球菌CCP污染的分析结果表明,鸡蛋、个人的手(农场工人、批发商和零售商)、货架上的鸡蛋和粪便是农场的CCP。
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引用次数: 0
Wild boar captured in a large corral-style trap or hunted: preliminary comparison of meat quality traits 在大型畜栏式陷阱中捕获或狩猎的野猪:肉质性状的初步比较
Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-11-08 DOI: 10.4081/ijfs.2023.11618
Caterina Altissimi, Eleonora Torregiani, Fausto Cambiotti, Rossana Roila, Raffaella Branciari, Samira Giovannini, David Ranucci
The management and numerical control of wild boars mainly depend on hunting practices, even if other alternative strategies such as the use of traps and cages can be adopted. There is little information available on the quality of captured wild boar meat. The aim of this study was to evaluate the meat quality of wild boars captured with a large corral-style trap compared to still hunting and collective hunting methods. Longissimus dorsi samples were collected from 60 wild boars, 20 of which were obtained by trapping, 20 by still hunting, and 20 by collective hunting. The animals considered were 32 males and 28 females, weighing between 42 and 68 kg. Muscle pH has been recorded after 1, 24, and 48 hours post-mortem. Furthermore, after 24 hours, color, drip loss, cooking loss, and Warner-Bratzler shear force were also evaluated. Trapping with large enclosures such as corral-style traps, if properly managed, does not seem to adversely affect the quality traits of wild boar meat, which were found to be like those obtained by the still hunting method.
野猪的管理和数量控制主要依靠狩猎活动,即使可以采用其他替代策略,如使用陷阱和笼子。关于捕获的野猪肉质量的信息很少。本研究的目的是比较用大型畜栏式陷阱捕获的野猪与静止狩猎和集体狩猎方法的肉质。采集60只野猪背最长肌标本,其中诱捕法20只,静捕法20只,集体狩猎法20只。研究对象为32只雄性和28只雌性,体重在42至68公斤之间。分别在死后1、24和48小时记录肌肉pH值。24小时后,观察颜色、滴漏损失、蒸煮损失和Warner-Bratzler剪切力。如果管理得当,用诸如畜栏式陷阱之类的大型围栏进行诱捕,似乎不会对野猪肉的质量性状产生不利影响,这些品质性状与用静止狩猎方法获得的品质性状相似。
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引用次数: 0
Lactic acid bacteria from artisanal raw goat milk cheeses: technological properties and antimicrobial potential 手工生羊奶奶酪中的乳酸菌:技术特性和抗菌潜力
Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-10-27 DOI: 10.4081/ijfs.2023.11559
Beatriz Nunes Silva, Nathália Fernandes, Laís Carvalho, Ana Sofia Faria, José António Teixeira, Carina Rodrigues, Ursula Gonzales-Barron, Vasco Cadavez
In cheese-making, a starter culture composed of adequately chosen lactic acid bacteria (LAB) may be suitable to ensure the rapid acidification of milk, improve textural and sensorial characteristics, and avoid pathogen proliferation. In this work, 232 LAB isolates collected from artisanal goat’s raw milk cheeses produced in Portugal were evaluated for their antimicrobial capacity (at 10 and 37°C), as well as their acidifying and proteolytic properties. Among the 232 isolates, at least 98% of those isolated in De Man-Rogosa-Sharpe (MRS) agar presented antagonism against Listeria monocytogenes, Salmonella Typhimurium, or Staphylococcus aureus, whereas less than 28.1% of M17-isolated LAB showed antagonism against these pathogens. M17-isolated LAB displayed better results than MRS ones in terms of acidifying capacity. As for the proteolytic assay, only two MRS isolates showed casein hydrolysis capacity. Principal component analyses and molecular characterization of a subset of selected isolates were conducted to identify those with promising capacities and to correlate the identified LAB genera and species with their antimicrobial, acidifying, and/or proteolytic properties. Lactococcus strains were associated with the highest acidifying capacity, whereas Leuconostoc and Lacticaseibacillus strains were more related to antimicrobial capacities. Leuconostoc mesenteroides, Lactococcus lactis, and Lacticaseibacillus paracasei were the predominant organisms found. The results of this work highlight various strains with pathogen inhibition capacity and suitable technological properties to be included in a customized starter culture. As future work, it is necessary to appropriately define the starter culture and implement it in the cheese-making process to evaluate if the in-vitro capacities are observable in a real food system.
在奶酪制作中,由适当选择的乳酸菌(LAB)组成的发酵剂可能适合确保牛奶的快速酸化,改善质地和感官特性,并避免病原体增殖。在这项工作中,从葡萄牙生产的手工山羊原料奶奶酪中收集了232株LAB菌株,对其抗菌能力(在10°C和37°C)以及酸化和蛋白水解特性进行了评估。在232株分离物中,至少98%的分离物对单核增生李斯特菌、鼠伤寒沙门菌或金黄色葡萄球菌有拮抗作用,而m17分离的LAB对这些病原菌的拮抗作用不到28.1%。m17分离LAB的酸化能力优于MRS分离LAB。在蛋白水解实验中,只有两株MRS菌株具有酪蛋白水解能力。通过主成分分析和分子特征分析,对部分分离菌株进行了鉴定,以确定哪些菌株具有良好的抗菌、酸化和/或蛋白水解性能,并将鉴定的LAB属和种与它们的抗菌、酸化和/或蛋白水解性能联系起来。乳球菌菌株具有最高的酸化能力,而Leuconostoc和乳酸菌菌株具有更高的抗菌能力。肠系膜白色球菌、乳酸乳球菌和副干酪乳杆菌是主要的微生物。这项工作的结果突出了各种菌株的病原体抑制能力和合适的技术特性,包括在定制的发酵剂培养。作为未来的工作,有必要适当地定义发酵剂并在奶酪制作过程中实施,以评估在实际食品系统中是否可以观察到体外能力。
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引用次数: 0
Occurrence and distribution of <i>Salmonella</i> serovars associated with human infection isolated from irrigation waters and food-producing animals in southern Italy: eleven-year monitoring (2011-2021) 沙门氏菌的发生和分布从意大利南部灌溉用水和粮食生产动物中分离出的与人类感染相关的血清型:11年监测(2011-2021年)
Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-10-26 DOI: 10.4081/ijfs.2023.11538
Maria Francesca Peruzy, Immacolata La Tela, Maria Rosaria Carullo, Simona Ioele, Yolande Thérèse Rose Proroga, Anna Balestrieri, Nicoletta Murru
Salmonella is one of the main zoonotic agents causing foodborne diseases in Europe. The main reservoirs of the infection are represented by domestic and wild animals, and the infection occurs by direct contact or following the consumption of contaminated food or water. The study aimed to evaluate the presence of Salmonella spp. in food-producing animals and irrigation waters in southern Italy and the serovar distribution. From 2011 to 2021, a total of 473 samples from 6 different animal species (bovine, buffalo, goat, ovine, swine, poultry, and wild boars) and 313 irrigation water samples were collected and analyzed. The overall percentage of positive samples was 56.87% in organs, 50.85% in feces, and 20.45% in irrigation waters. By animal species, the most frequently detected serovar was Salmonella Typhimurium in bovine (17.39%), in buffalo (13.10%) and swine (28.21%), and S. Kentucky (24.78%) in poultry. The subspecies diarizonaeIIIb was frequently detected in goats (40.00%) and ovine (83.33%), while salamaeII (14.12%) and diarizonaeIIIb (11.76%) were frequently isolated in wild boars. In the irrigation water samples, the most frequently detected serovar was S. Napoli (25%). Results revealed that, although in Europe, control strategies aimed at preventing the spread of Salmonella have been implemented, the prevalence of this pathogen in food-producing animals and irrigation waters is high. Considering the risk to public health associated with the contamination of products or foods, more stringent control interventions are needed at primary production and along the food chain.
沙门氏菌是欧洲引起食源性疾病的主要人畜共患病原体之一。感染的主要宿主是家畜和野生动物,感染是通过直接接触或食用受污染的食物或水而发生的。本研究旨在评估意大利南部粮食生产动物和灌溉用水中沙门氏菌的存在及其血清分布。2011 - 2021年共采集了牛、水牛、山羊、绵羊、猪、家禽和野猪6种动物样本473份,灌溉水样本313份。总体阳性检出率为器官56.87%,粪便50.85%,灌溉水20.45%。按动物种类分,检出率最高的是牛鼠伤寒沙门菌(17.39%)、水牛沙门菌(13.10%)和猪沙门菌(28.21%),家禽沙门菌(24.78%)。山羊(40.00%)和绵羊(83.33%)中常检出diarizonaeIIIb亚种,野猪中常检出salamaeII(14.12%)和diarizonaeIIIb(11.76%)亚种。在灌溉水样品中,最常见的血清型是S. Napoli(25%)。结果显示,尽管在欧洲已实施了旨在防止沙门氏菌传播的控制策略,但该病原体在食品生产动物和灌溉水中的流行率很高。考虑到与产品或食品污染有关的公共卫生风险,需要在初级生产和食物链上采取更严格的控制干预措施。
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引用次数: 0
<i>Trichinella</i> spp. detection in hunted wild boar (<i>Sus scrofa</i>) diaphragm biopsies in Central Italy & lt; i&gt Trichinella&lt; / i&gt;意大利中部被猎杀野猪(&lt;i&gt;Sus scrofa&lt;/i&gt;)横膜活检中的spp检测
Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-10-24 DOI: 10.4081/ijfs.2023.11467
Andrea Piccinini, Daniela Ronconi, Assunta De Luca, Valentina D’Ovidio, Gianluigi Ferri, Alberto Vergara
Trichinellosis is a globally diffused food-borne parasitic disease caused by nematodes of the Trichinella complex. During evolution, guided by ecological interactions, natural selection, and biochemical “intelligence”, these parasites developed admirable strategies to infect the host’s organism. One of the most fascinating is represented by the nurse cell formation in muscular tissue (e.g., diaphragm, skeletal muscle, extrinsic ocular muscles, etc.). This strategy allowed the parasite to adapt and conquer the wider host species spectrum, including ungulates and humans. Consumption of undercooked meat from infected wild ungulates constitutes the most important source of infection for the human species. In this study, we show the prevalence of Trichinella spp. in hunted wild boars (Sus scrofa) in Central Italy. During the hunting season 2021/2022 in the Province of Rieti, 554 wild boar diaphragm biopsies were collected for Trichinella spp. screening, in accordance with Regulation EU 1375/2015. An artificial digestion method was used for the detection of Trichinella spp. larval forms. The results revealed a positivity of 0.18% (1/554), and molecular biology identification demonstrated the presence of Trichinella britovi in the positive sample. This species is the most diffused in wild ungulate populations in Central Italy and the most frequently isolated in human patients with trichinellosis from this area, showing a close epidemiological relation between Homo sapiens and Sus scrofa for Trichinella spp. diffusion in an ecosystem. Epidemiological surveillance, in receptive animal species destined for human consumption and at any One Health level, represents the main “winning” strategy in the control of this worldwide, widespread food-borne parasitic disease.
旋毛虫病是由旋毛虫复合体线虫引起的一种全球传播的食源性寄生虫病。在进化过程中,在生态相互作用、自然选择和生化“智能”的指导下,这些寄生虫发展出了令人钦佩的策略来感染宿主的有机体。其中最引人注目的是肌肉组织(如横膈膜、骨骼肌、外源性眼肌等)中护士细胞的形成。这种策略使寄生虫能够适应并征服更广泛的宿主物种,包括有蹄类动物和人类。食用未煮熟的受感染野生有蹄类动物的肉是人类最重要的感染源。在这项研究中,我们展示了旋毛虫在意大利中部被猎杀野猪(Sus scrofa)中的流行情况。在Rieti省2021/2022年狩猎季节,根据欧盟1375/2015条例,收集了554份野猪横膈膜活检标本,用于旋毛虫筛查。采用人工消化法检测旋毛虫幼虫。检测结果为0.18%(1/554),分子生物学鉴定证实阳性样品中存在布氏旋毛虫。该物种在意大利中部野生有蹄类动物种群中分布最广,在该地区人类旋毛虫病患者中最常分离到,表明在生态系统中旋毛虫传播方面,智人与苏氏螺旋体之间存在密切的流行病学关系。在供人类食用的易受感染动物物种中进行流行病学监测,是控制这种世界范围内广泛传播的食源性寄生虫病的主要“制胜”战略。
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Italian Journal of Food Safety
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