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Effect of packaging and storage conditions on some quality traits of bovine meat. 包装和贮存条件对牛肉某些品质性状的影响。
IF 1.3 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-06-21 DOI: 10.4081/ijfs.2022.10038
Luca Grispoldi, Athanasios Chalias, Eleonora Barzi, Laura Pecorari, Marco Tassinari, Cristina Saraiva, Juan García-Díez, Musafiri Karama, Saeed El-Ashram, Giovanna Traina, Beniamino T Cenci-Goga

Packaging is considered one of the most interesting technological aspects of food production and is a constantly evolving subject in food production. The type of packaging is important for the quality and safety of the product and for the visual appearance of the product to be immediately evaluated by consumers. The purpose of this study was to investigate the effect of four different types of modified atmosphere packaging (ATM) and vacuum packaging (VP) currently used by a company in central Italy, on the main qualitative characteristics of beef. For these two traditional and two new solutions with reduced environmental impact and compostable were evaluated. For each type of packaging, two different products were analyzed: steaks and hamburgers. The samples, immediately after production, were transported to the laboratory in refrigerated containers. Several parameters (color, pH, water holding capacity, drip loss, and microbiological characteristics) were evaluated at time 0 and after 7 (T7), 14 (T14) and 21 days (T21) of storage in the dark and at refrigeration temperature (+4°C ± 2°C). The results showed that the two types of packaging have very similar effects on the water-retaining capacity of the steaks. More noticeable differences were recorded by the colorimetric analysis: for both steaks and hamburgers, the products packaged in the traditional packaging appeared brighter and redder than those packaged in the new alternatives. The microbiological analysis of the steaks showed higher values in the "new" packaging. The formation of abundant ropy slime was observed in one of the samples in the "new" modified atmosphere package at T21. The results of this study showed that the technological characteristics (in particular, the color) and the microbiological characteristics of the steaks and hamburgers were better in "old" packaging, with a better appearance and a longer shelf life. The results obtained show how the research for eco-sustainable products for packaging must be addressed, taking into account the effect of the materials on the qualitative and hygienic- sanitary characteristics of the meat.

包装被认为是食品生产中最有趣的技术方面之一,是食品生产中不断发展的主题。包装的类型对产品的质量和安全以及消费者立即评价产品的视觉外观都很重要。本研究的目的是调查意大利中部一家公司目前使用的四种不同类型的改性气氛包装(ATM)和真空包装(VP)对牛肉主要质量特征的影响。对这两种传统解决方案和两种新的解决方案进行了评估,以减少对环境的影响和可堆肥。对于每种包装,分析了两种不同的产品:牛排和汉堡。样品在生产后立即用冷藏容器运送到实验室。在0、7 (T7)、14 (T14)和21天(T21)的黑暗和冷藏温度(+4°C±2°C)下,评估几个参数(颜色、pH、持水量、滴漏损失和微生物特性)。结果表明,两种包装方式对牛排保水性能的影响非常相似。比色分析记录了更明显的差异:对于牛排和汉堡,用传统包装包装的产品看起来比用新替代品包装的产品更亮、更红。对牛排的微生物分析显示,“新”包装中的微生物含量更高。在T21的“新”改性气氛包中的一个样品中观察到大量粘稠黏液的形成。本研究结果表明,“旧”包装的牛排和汉堡包的工艺特性(特别是颜色)和微生物特性更好,外观更好,保质期更长。所获得的结果表明,如何研究生态可持续产品的包装必须解决,考虑到材料对肉的质量和卫生-卫生特性的影响。
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引用次数: 0
Evolution of ß-lactams, fluroquinolones and colistin resistance and genetic profiles in Salmonella isolates from pork in northern Italy. 意大利北部猪肉沙门氏菌分离株中ß-内酰胺类、氟喹诺酮类和粘菌素耐药性的进化和基因谱。
IF 1.3 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-06-21 DOI: 10.4081/ijfs.2022.9972
Ilaria Carmosino, Silvia Bonardi, Martina Rega, Andrea Luppi, Luca Lamperti, Maria Cristina Ossiprandi, Cristina Bacci

The European Food Safety Authority and European Centre of Disease Prevention and Control antimicrobial resistance report published in 2021 shows increasing levels of antimicrobial resistance in Salmonella against antibiotics of choice for human salmonellosis (s-lactams and fluoroquinolones). The aim of the study was to follow the evolution of resistance against some Critical Important Antimicrobials in Salmonella isolates from fresh pork collected in Emilia-Romagna region, northern Italy, over two decades. Emilia-Romagna region is characterized by production of well-known pork derived products, as Parma Ham. The samples were collected in three different periods, ranging from 2000 to 2003, 2012 to 2016 and 2018 to 2021. After serotyping, the isolates were phenotypically tested for resistance to three classes of antibiotics: s-lactams, fluoroquinolones and polymyxins. End-point polymerase chain reaction (PCR) and PCRReal Time were used for genotypical analyses. The phenotypical resistance to s-lactams and fluoroquinolones were clearly increasing when comparing the results obtained from isolates collected in the first period (16.7% and 16.7%, respectively) with those of the third period (29.7% and 32.4%, respectively). On the contrary, the resistance to colistin decreased from 33.3% to 5.4%. Genotypically, the 71.4% and 83.3% of the strains harboured s-lactams and fluoroquinolones genes, respectively, while colistin resistance genes were not detected in the phenotypically resistant strains.

欧洲食品安全局和欧洲疾病预防和控制中心于2021年发表的抗微生物药物耐药性报告显示,沙门氏菌对人类沙门氏菌病所选抗生素(s-内酰胺类和氟喹诺酮类)的耐药性水平不断上升。这项研究的目的是跟踪20多年来从意大利北部艾米利亚-罗马涅地区收集的新鲜猪肉中分离出的沙门氏菌对一些重要抗菌素的耐药性演变。艾米利亚-罗马涅地区的特点是生产著名的猪肉衍生产品,如帕尔马火腿。样本是在2000年至2003年、2012年至2016年和2018年至2021年三个不同时期收集的。经血清分型后,对三种抗生素(s-内酰胺类、氟喹诺酮类和多粘菌素)的耐药性进行表型检测。采用终点聚合酶链反应(PCR)和PCR real Time进行基因型分析。与第一期(16.7%和16.7%)和第三期(29.7%和32.4%)比较,对s-内酰胺类和氟喹诺酮类药物的表型耐药明显增加。相反,对粘菌素的耐药率从33.3%下降到5.4%。表型耐药菌株中未检出粘菌素耐药基因,71.4%和83.3%的菌株分别携带s-内酰胺类和氟喹诺酮类基因。
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引用次数: 0
Evaluation of beef in purified sea water: microbiological and chemical-physical aspects 净化海水中牛肉的评价:微生物学和化学物理方面
IF 1.3 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-02-22 DOI: 10.4081/ijfs.2022.10034
S. Stella, C. Bernardi, Davide Timaco, E. Tirloni
In this study the influence of the maintenance of beef (Bavarian heifer meat submitted to dry aging for 1 month) in purified sea water was evaluated considering microbiological and physical-chemical parameters. The treated samples were perforated on their upper and lower surfaces, subsequently placed in sterile plastic containers, and covered with purified sea water while the control samples were wrapped in a plastic film; all the samples were stored at 2°C and sample analyses were performed on the day of receipt (t0) and after five (t5), seven (t7) and ten (t10) days of storage. The initial bacterial population was close to 6 log CFU/g, as expected in meat subjected to a long ageing. After 5 and 10 days of storage the treated samples showed significantly lower counts for most parameters (Total Bacterial Count, Enterobacteriaceae, Lactic Acid Bacteria and Pseudomonas spp.) if compared to the control samples. Halotolerant bacteria, yeasts and moulds showed no differences between the two series. During the test, the exposed surface of treated meat samples underwent a partial protein denaturation. Analyses show a moderate acidification of the meat during the first part of storage (from t0 to t5), followed by a substantial stabilization in the second part of the trial. The meat maintained in purified sea water acquired a significantly and constantly higher water retention due to the absorption of salt by the meat. No significant difference was detected in terms of tenderness between the two series.
在本研究中,考虑到微生物和物理化学参数,评估了牛肉(巴伐利亚小母牛肉经过1个月的干陈处理)在纯净海水中保持的影响。将处理过的样品在其上下表面穿孔,随后放置在无菌塑料容器中,并用纯化的海水覆盖,同时将对照样品包裹在塑料膜中;所有样品在2°C下储存,并在收到当天(t0)以及储存五(t5)、七(t7)和十(t10)天后进行样品分析。最初的细菌种群接近6 log CFU/g,正如在经过长时间老化的肉类中预期的那样。储存5天和10天后,与对照样品相比,处理过的样品在大多数参数(细菌总数、肠杆菌科、乳酸菌和假单胞菌属)方面显示出显著较低的计数。耐盐细菌、酵母和霉菌在两个系列之间没有差异。在试验过程中,处理过的肉类样品的暴露表面发生了部分蛋白质变性。分析表明,在储存的第一部分(从t0到t5),肉发生了适度的酸化,随后在试验的第二部分基本稳定。由于肉对盐的吸收,保持在纯净海水中的肉获得了显著且持续较高的保水性。两个系列在压痛方面没有发现显著差异。
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引用次数: 0
The effect of food safety training program on food safety knowledge and practices in hotels’ and hospitals’ food services 食品安全培训计划对酒店和医院食品服务中食品安全知识和实践的影响
IF 1.3 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-02-22 DOI: 10.4081/ijfs.2022.9914
H. Al-Akash, Abdelhakeem Abu Arrah, Feyza Bhatti, Roqia Maabreh, Riham Abu Arrah
Food Safety Knowledge (FSK) among Food Handlers (FHS) in the food services in both hotels and hospitals has a crucial role in food protection and combating against foodborne diseases. The aim is to check the food safety (FS) training program upon FSK in the Jordanian hotels’ and hospitals’ food services. A pre-test-post-test design was used. A convenience sample of 412 food handlers from randomly selected hotels and hospitals were recruited. Data were collected using 118-questions Food Safety Knowledge Questionnaire (FSKQ); 40- questions tool for FS Practices. A significant improvement in FSK mean scores in the post-test (85.48±17.07/118 points corresponded to 72.44%) compared with the pretest (78.50±13.69/118 points, corresponding to 66.66%). The overall mean FSK score was considered to be “moderate” in pre and post-tests. FS Practice level mean was 26.86±4.38/40 points (67.14%), regarded as “moderate” in the pre-test and continued to be “moderate” after the training despite a significant increase in the post-test scores (27.92±4.45/40 point, corresponded to 69.80%, P=0.000). FHS worked in organizations where HACCP principles are implemented, possessed higher FSK and better FS practices. Although continued to be “moderate”, FSK and FS Practices among FHS in the Jordanian hotels and hospitals have been significantly improved after the FS Training Program.
酒店和医院食品服务部门的食品处理人员的食品安全知识在食品保护和抗击食源性疾病方面发挥着至关重要的作用。目的是检查约旦酒店和医院食品服务部门的食品安全(FS)培训计划。采用前测后测设计。从随机选择的酒店和医院招募了412名食品处理人员作为方便样本。采用118题食品安全知识问卷(FSKQ)收集数据;40个问题的FS实践工具。测试后FSK平均得分(85.48±17.07/118分,对应72.44%)较测试前(78.50±13.69/118分,对应66.66%)有显著提高。在测试前和测试后,总体平均FSK分数被认为是“中等”。FS练习水平平均值为26.86±4.38/40分(67.14%),前测为“中等”,训练后得分明显提高(27.92±4.45/40分,对应69.80%,P=0.000),训练后仍为“中等”。FHS在实施HACCP原则的组织中工作,拥有更高的FSK和更好的FS实践。虽然在约旦酒店和医院的家庭卫生服务人员中,家庭卫生服务人员的家庭卫生服务和家庭卫生服务的做法仍然是“适度的”,但在家庭卫生服务培训方案之后,已经有了显著改善。
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引用次数: 7
Collection and analysis of post mortem inspection outcomes (liver lesions) from different cattle slaughtering plants located in Northern and Southern Italy 收集和分析意大利北部和南部不同牛屠宰场的死后检查结果(肝脏损伤)
IF 1.3 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-02-22 DOI: 10.4081/ijfs.2022.10035
S. Stella, E. Tirloni, C. Bernardi, Greta Acerbis, Teresa Manginelli
The study evaluated the outcomes of cattle liver postmortem examination (64,766 animals) obtained from three slaughterhouses (two located in Lombardy, Northern Italy, and one in Puglia, Southern Italy) through the period 2016-2020. The frequency of specific lesions determining liver condemnation was calculated and the influence of several factors (animal age/category, geographical area, season, plant) was considered. A mean prevalence of 8% was observed, with a significant difference among the plants (range 6.4-12.8%). A significant difference was observed among the animal categories and age classes, with higher condemnation rates in animals aged more than 30 months (mainly cows). Steatosis was the most frequent lesion observed in cows (about half of the total), whereas liver abscesses were the most diffused in younger animals (half of the total in young bulls). Other frequently observed lesions were distomatosis and perihepatitis. A different pattern was observed between the two geographical areas, with a higher prevalence of steatosis in Lombardy (mainly intensive dairy farming), and of distomatosis in Puglia (animals mainly grazed on pasture). The season influenced the prevalence of lesions, and especially of steatosis (higher in summer). A different lesion prevalence was also observed between the two plants on similar animal populations, suggesting a difference in the notification and classification procedure among the plants. This study highlights the importance of a proper sharing procedure of the information obtained from the post mortem inspection in order to facilitate an optimal use of Food Chain Information and a useful feedback for farmers.
该研究评估了2016-2020年期间从三个屠宰场(两个位于意大利北部伦巴第,一个位于意大利南部普利亚)获得的牛肝脏尸检结果(64,766头动物)。计算特定病变决定肝脏谴责的频率,并考虑几个因素(动物年龄/类别、地理区域、季节、植物)的影响。平均感染率为8%,不同植物间差异显著(6.4 ~ 12.8%)。在动物类别和年龄层之间存在显著差异,30月龄以上的动物(主要是奶牛)的谴责率较高。脂肪变性是奶牛中最常见的病变(约占总数的一半),而肝脓肿在年轻动物中最普遍(占年轻公牛总数的一半)。其他常见的病变是口臭和周围肝炎。在两个地理区域之间观察到不同的模式,伦巴第(主要是集约化奶牛养殖)和普利亚(主要在牧场上放牧的动物)的脂肪变性患病率较高。季节影响病变的发生率,尤其是脂肪变性(夏季较高)。在相似的动物种群中,两种植物的病变发生率也不同,这表明植物之间的通报和分类程序存在差异。本研究强调了从死后检验中获得的信息的适当共享程序的重要性,以促进食物链信息的最佳利用,并为农民提供有用的反馈。
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引用次数: 0
Presence of enteric bacterial pathogens in meat samples of wild boar hunted in Campania region, southern Italy 意大利南部坎帕尼亚地区狩猎的野猪肉样本中存在肠道细菌病原体
IF 1.3 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-02-22 DOI: 10.4081/ijfs.2022.9967
M. F. Peruzy, D. Cristiano, E. Delibato, N. D’Alessio, Y. Proroga, Rosaria Luana Capozza, Antonio Rippa, N. Murru
Wild boars can be infected with several foodborne pathogens which may be transmitted to humans through the consumption of their meat, but currently, data of their prevalence are still limited. The present study aimed to evaluate the presence of enteric pathogens in wild boar meat samples killed in the Campania region. Twentyeight wild boar meat samples were analyzed for the detection of Salmonella spp, Y. enterocolitica, Campylobacter spp., and Shiga- Toxigenic E. coli. Salmonella spp. was detected and isolated in ten samples and after serotyping S. Veneziana, S. Kasenyi, S. Coeln, S. Manhattan, S. Thompson, and S. Stanleyville were identified. Twenty-one meat samples were found to be contaminated with Y. enterocolitica; in 6 samples the ystA and ystB genes were detected simultaneously, while in 15 only the ystB gene, which characterizes the bacteria belonging to the biotype 1A, was present. Shiga-Toxin producing E. coli was detected in 12 while Campylobacter spp was never detected. In conclusion, due to the high occurrence of pathogenic bacteria detected, the present research shows that wild boars are important reservoirs for foodborne zoonoses which may be transmitted to livestock and humans. This confirms the importance of controls throughout the wild boar supply chain. In the Campania region, checks are guaranteed by the Veterinarians who work within the “management and control plan for wild boar in the Campania region” which has the twofold objective of containing the increasingly invasive presence of this animal and guaranteeing greater safety, traceability, and transparency in the consumption of meat.
野猪可能感染几种食源性病原体,这些病原体可能通过食用其肉类传播给人类,但目前,关于其流行率的数据仍然有限。本研究旨在评估坎帕尼亚地区杀死的野猪肉样本中是否存在肠道病原体。对28份野猪肉样本进行了沙门氏菌、小肠结肠炎杆菌、弯曲杆菌和志贺毒素大肠杆菌的检测分析。在10个样本中检测并分离出沙门氏菌,并在对S.Veneziana、S.Kasenyi、S.Coeln、S.Manhattan、S.Thompson和S.Stanleyville进行血清分型后进行鉴定。21份肉类样本被发现被小肠结肠炎杆菌污染;在6个样品中同时检测到ystA和ystB基因,而在15个样品中仅存在ystB基因(其特征是属于生物型1A的细菌)。产志贺毒素的大肠杆菌在12株中被检测到,而弯曲杆菌从未被检测到。总之,由于检测到的致病菌发生率很高,目前的研究表明,野猪是食源性人畜共患疾病的重要宿主,这些疾病可能会传播给牲畜和人类。这证实了控制整个野猪供应链的重要性。在坎帕尼亚地区,兽医在“坎帕尼亚区域野猪管理和控制计划”范围内进行检查,该计划的双重目标是遏制这种动物日益入侵的存在,并确保肉类消费的更大安全性、可追溯性和透明度。
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引用次数: 4
Development of a predictive model for the shelf-life of Atlantic mackerel (Scomber scombrus) 大西洋鲭鱼(scober scorbrus)货架期预测模型的建立
IF 1.3 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-02-22 DOI: 10.4081/ijfs.2022.10019
F. Giarratana, F. Panebianco, Luca Nalbone, G. Ziino, David A. Valenti, A. Giuffrida
Despite its commercial value, the shelflife of the Atlantic mackerel (Scomber scombrus) during refrigerated storage was poorly investigated. In this regard, the Quality Index Method (QIM) was proposed as a suitable scoring system for freshness and quality sensorial estimation of fishery products. This study aims to develop a deterministic mathematical model based on dynamic temperatures conditions and a successive statistical analysis of the results obtained. This model will be exploited to predict the shelf-life of the Atlantic mackerel based on specific storage temperatures. A total of 60 fresh fishes were subdivided into two groups and respectively stored in ice for 12 days at a constant temperature of 1±0.5°C (Group A) and a fluctuating temperature ranging between 1 and 7°C (Group B). Microbiological analysis and sensory evaluation through the QIM were performed on each fish at regular time intervals. A critical value of 6 Log cfu/g of spoilage bacteria (mainly psychotropic) associated with a significant decay of the sensorial characteristics was exceeded after 9 days of storage for Group A and 3 days for Group B. A reliable prediction of fish freshness was obtained by modelling the QIM as a function of the spoilage bacteria behaviour. A coefficient β of correlation was determined to convert the spoilage bacteria load into a Quality Index score. The adoption of mathematical predictive models to assess microbial behaviour under different environmental conditions is an interesting tool for food industries to maximize production and reduce waste.
尽管其商业价值,大西洋鲭鱼(Scomber scorbrus)在冷藏储存期间的货架期调查甚少。在此基础上,提出了质量指数法(QIM)作为水产品新鲜度和质量感官评价的评分体系。本研究旨在建立一个基于动态温度条件的确定性数学模型,并对所得结果进行连续统计分析。这个模型将被用来预测大西洋鲭鱼的保质期基于特定的储存温度。将60条鲜鱼分成两组,分别在1±0.5°C的恒温条件下(A组)和1 ~ 7°C的波动温度下(B组)冷藏12天。每隔一段时间对每条鱼进行微生物学分析和QIM感官评价。A组和b组分别在储存9天和3天后超过了与感官特征显著衰减相关的6 Log cfu/g腐败细菌(主要是精神药物)的临界值。通过将QIM建模为腐败细菌行为的函数,获得了对鱼类新鲜度的可靠预测。确定了相关系数β,将腐败菌负荷转化为质量指数得分。采用数学预测模型来评估微生物在不同环境条件下的行为是食品工业最大化生产和减少浪费的一个有趣的工具。
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引用次数: 1
Antimicrobial and anti-biofilm activity of manuka essential oil against Listeria monocytogenes and Staphylococcus aureus of food origin 麦卢卡精油对食品源性李斯特菌和金黄色葡萄球菌的抑菌和抗生物膜活性研究
IF 1.3 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-02-22 DOI: 10.4081/ijfs.2022.10039
F. Pedonese, Eleonora Longo, B. Torracca, B. Najar, F. Fratini, R. Nuvoloni
The activity of manuka (Leptospermum scoparium) essential oil (EO) on biofilms of foodborne Listeria monocytogenes and Staphylococcus aureus has been studied. Seven strains of L. monocytogenes and 7 of S. aureus (5 methicillin-resistant) were tested. EO minimal inhibitory concentration (MIC), EO minimal bactericidal concentration (MBC) and biofilm production quantification were determined for each strain by microtiter methods. Moreover, EO Minimum Biofilm Inhibitory Concentration (MBIC) and Minimum Biofilm Eradicating Concentration (MBEC) were determined on 2 L. monocytogenes and 3 S. aureus that showed the best biofilm production. Finally, on 4 strains out of 5 (2 L. monocytogenes and 2 S. aureus) EO Biofilm Reduction Percentage (BRP) vs. untreated controls was assessed after a treatment with EO subinhibitory concentrations. The chemical composition of manuka essential oil was determined by Gas Chromatography- Electron Impact Mass Spectrometry (GCEIMS). The manuka EO demonstrated good antimicrobial activity: L. monocytogenes MIC and MBC were 0.466 mg/ml and 0.933 mg/ml, respectively, whereas S. aureus MIC and MBC were 0.233 mg/ml and 0.466 mg/ml, respectively. Furthermore, L. monocytogenes showed a MBIC of 0.933 mg/ml and a MBEC in the range of 0.933–1.865 mg/ml, whereas S. aureus had a MBIC in the range of 7.461–14.922 mg/ml and a MBEC of 14.922 mg/ml. L. monocytogenes revealed no significant BRP after the treatment with manuka EO, whereas S. aureus showed a BRP higher than 50% with MIC/2 and MIC/4 EO concentrations. These results provide information for feasible manuka EO applications in food production systems.
研究了麦卢卡精油对食源性单核细胞增多性李斯特菌和金黄色葡萄球菌生物膜的活性。检测了7株单核细胞增多性李斯特菌和7株金黄色葡萄球菌(耐5甲氧西林)。通过微量滴定法测定每个菌株的EO最小抑制浓度(MIC)、EO最小杀菌浓度(MBC)和生物膜产生量。此外,在2株单核细胞增多性李斯特菌和3株金黄色葡萄球菌上测定了EO最小生物膜抑制浓度(MBIC)和最小生物膜清除浓度(MBEC),这两株菌株表现出最佳的生物膜产量。最后,在用EO亚抑制浓度处理后,对5个菌株中的4个菌株(2个单核细胞增多性李斯特菌和2个金黄色葡萄球菌)的EO生物膜减少百分比(BRP)与未处理的对照进行了评估。采用气相色谱-电子碰撞质谱法(GCEIMS)测定了麦卢卡精油的化学成分。麦卢卡EO表现出良好的抗菌活性:单核细胞增多性李斯特菌的MIC和MBC分别为0.466mg/ml和0.933mg/ml,而金黄色葡萄球菌的MIC或MBC分别是0.233mg/ml和0.466mg/ml。此外,单核细胞增多性李斯特菌的MBIC为0.933 mg/ml,MBEC在0.933–1.865 mg/ml范围内,而金黄色葡萄球菌的MBIC在7.461–14.922 mg/ml范围外,MBEC为14.922 mg/ml。单核细胞增多性李斯特菌在用麦卢卡EO处理后没有显示出显著的BRP,而金黄色葡萄球菌在MIC/2和MIC/4 EO浓度下显示出高于50%的BRP。这些结果为麦卢卡EO在食品生产系统中的可行应用提供了信息。
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引用次数: 1
European legislation and live bivalve molluscs: Are the criteria for microbiological safety matching with the criteria for sanitary classification of harvesting areas? 欧洲立法和活的双壳类软体动物:微生物安全标准是否与采集区卫生分类标准相匹配?
IF 1.3 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-02-22 DOI: 10.4081/ijfs.2022.9956
C. Ciccarelli, M. Leinoudi, A. M. Semeraro, Vittoria Di Trani, Elena Ciccarelli, Gaia Consorti
The European Union (EU) established the criteria for the classification of shellfish harvesting areas, based on the results of monitoring E. coli in shellfish. The EU also defined E. coli as a microbiological criterion for end product safety, based on a three-class sampling plan. Both criteria are based on the MPN method, a test with a statistical approach and in which different factors contribute to the variability of the outcomes. This theoretical study, based on combinatorics and Bayes’ theorem for conditional probability, investigates the consistency between these two criteria and aims to determine the probability of obtaining false compliant and non-compliant results when applying the safety criterion test to shellfish placed on the market. The results show that in the second case, the probability of non-compliant outcomes does not appear negligible within a range between 10% and 50% in the different hypothesized scenarios, with a probability of false noncompliant outcomes over 10%. In addition, the Bayes’ Theorem shows that Class A, or Class B areas (as allowed), could be the origin of non-compliant shellfish, with a not negligible frequency. Therefore, within the limits of the assumed working hypotheses, the safety criterion for E. coli, as described in Regulation EC/2073/2005, does not appear to be consistent and coordinated with the classification criteria stated in the Regulation EU/2019/627 and it is not closely related to the sanitary status of shellfish harvesting area.
欧洲联盟(欧盟)根据贝类中大肠杆菌的监测结果,制定了贝类捕捞区的分类标准。欧盟还根据三级抽样计划,将大肠杆菌定义为最终产品安全的微生物标准。这两个标准都基于MPN方法,这是一种采用统计方法的测试,其中不同的因素会导致结果的可变性。这项理论研究基于组合数学和条件概率的贝叶斯定理,研究了这两个标准之间的一致性,旨在确定在将安全标准测试应用于市场上的贝类时,获得虚假符合和不符合结果的概率。结果表明,在第二种情况下,在不同的假设场景中,不符合结果的概率在10%至50%之间的范围内似乎不可忽略,虚假不符合结果概率超过10%。此外,贝叶斯定理表明,A类或B类区域(如允许)可能是不合规贝类的起源地,其频率不可忽略。因此,在假设的工作假设范围内,条例EC/2073/2005中描述的大肠杆菌安全标准似乎与条例EU/2019/627中描述的分类标准不一致和不协调,也与贝类捕捞区的卫生状况没有密切关系。
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引用次数: 0
Temperature fluctuations along food supply chain: A dynamic and stochastic predictive approach to establish the best temperature value in challenge tests for Listeria monocytogenes 食品供应链中的温度波动:在单核增生李斯特菌攻毒试验中建立最佳温量值的动态和随机预测方法
IF 1.3 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-02-22 DOI: 10.4081/ijfs.2022.9981
F. Giarratana, Luca Nalbone, G. Ziino, Giorgio Donato, S. Marotta, Filippa Lamberta, A. Giuffrida
This study aims to evaluate the behaviour of Listeria monocytogenes under fluctuating temperature comparing the efficacy of deterministic and stochastic methods for its prediction. In the first part of the study, a strain of L. monocytogenes was maintained at two different fluctuating temperature regimes both from 2 to 8°C and regularly sampled for the quantitative determination. The first temperature regime lasted 204 hours with a fluctuation length of 12 hours whereas the second lasted 167 hours with a fluctuation length of 24 hours. A dynamic predictive model was implemented for the reproduction of the observed data. Model resolution has been carried out by using values of the recorded temperature as well as the value of the mean temperature, the kinetic mean temperature, the 75th and 95th percentile of the temperature. A stochastic resolution was also performed considering the mean temperature and Standard Deviation as stochastic variable. In the second part of the study, a temperature mean curve was constructed by monitoring temperature of 8 refrigerated conveyances, 10 display cabinet and 15 domestic refrigerators. This curve was used to obtain predictive scenarios for L. monocytogenes based on the above and also considering temperature regime suggested by the EURL Lm TECHNICAL GUIDANCE DOCUMENT on challenge tests and durability studies for assessing shelf-life of ready-to-eat foods related to Listeria monocytogenes (Version 4 of 1 July 2021). All predicted behaviours were compared to the observed ones through the Root Mean Squared Error. Firstly, dynamic predictive model as well as the stochastic one, provided the best level of reproducibility of the observed data. The kinetic mean temperature reproduced the observed data better than the mean temperature for the 12 hoursregime while for the 24 hours-regime was the opposite. The 75th and 95th percentile overestimated the observed growths. Secondary, predictions obtained with the mean temperature, kinetic temperature and stochastic approach well fitted the observed data. The 75th and 95th percentile of Temperature and the “Eurl LM” temperature regimes overestimated the observed prediction. Dynamic approach as well as the stochastic one allowed to obtain the lowest values of Root Mean Squared Error. The mean temperature and kinetic mean temperature appeared the most representative values in a deterministic “single-point” approach.
本研究旨在评估单核细胞增多性李斯特菌在波动温度下的行为,比较确定性和随机性方法对其预测的有效性。在研究的第一部分中,将一株单核细胞增多李斯特菌维持在2至8°C的两种不同的波动温度条件下,并定期取样进行定量测定。第一种温度持续204小时,波动长度为12小时,而第二种温度持续167小时,波动时间为24小时。为了再现观测数据,实现了一个动态预测模型。模型分辨率是通过使用记录的温度值以及平均温度、动力学平均温度、温度的第75和第95百分位数的值来实现的。将平均温度和标准偏差作为随机变量进行了随机求解。在研究的第二部分中,通过监测8台冷藏运输工具、10台展示柜和15台家用冰箱的温度,构建了温度平均曲线。该曲线用于获得基于上述情况的单核细胞增多性李斯特菌的预测情景,并考虑了EURL Lm技术指导文件中关于评估与单核细胞增生性李斯特菌相关的即食食品保质期的挑战试验和耐久性研究(2021年7月1日第4版)所建议的温度条件。通过均方根误差将所有预测的行为与观察到的行为进行比较。首先,动态预测模型和随机预测模型提供了观测数据的最佳再现性。动力学平均温度比12小时制度的平均温度更好地再现了观察到的数据,而24小时制度则相反。第75和第95百分位高估了观察到的增长。其次,用平均温度、动力学温度和随机方法获得的预测与观测数据非常吻合。温度的第75和第95百分位以及“Eurl LM”温度状态高估了观测到的预测。动态方法和随机方法都允许获得最小的均方根误差值。在确定性“单点”方法中,平均温度和动力学平均温度出现了最具代表性的值。
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引用次数: 3
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Italian Journal of Food Safety
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